AlfalfaGEDB | Msa0145450

M. sativa cultivar zhongmu-4 / Msa0145450

KEGG: -
GO: GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
PFAM: PF02362.24

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0145450	XP_013466025.1	83.602	372	23	1	1	334	1	372	0.0	627

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0145450	sp\|Q8RYD1\|REM16_ARATH	34.675	323	177	8	8	315	11	314	1.17e-47	166

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0145450	A0A072VDG5	83.602	372	23	1	1	334	1	372	0.0	627

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TFs/TRs:

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Gene ID	Type	Classification
Msa0145450	TF	B3

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0061080	Msa0145450	0.801142	1.035285e-48	-8.615850e-47
Msa0101600	Msa0145450	0.802491	5.471531e-49	-8.615850e-47
Msa0105620	Msa0145450	0.801559	8.505339e-49	-8.615850e-47
Msa0145450	Msa0262050	0.802461	5.548999e-49	-8.615850e-47
Msa0145450	Msa0388700	0.801808	7.560641e-49	-8.615850e-47
Msa0145450	Msa0660490	0.822863	1.886967e-53	-8.615850e-47
Msa0145450	Msa0677290	0.802855	4.602654e-49	-8.615850e-47
Msa0145450	Msa0867210	0.870164	1.794590e-66	-8.615850e-47
Msa0145450	Msa0867220	0.807001	6.255950e-50	-8.615850e-47
Msa0145450	Msa1009820	0.804390	2.210507e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0145450	MtrunA17_Chr1g0154191	83.602	372	23	1	1	334	1	372	0.0	627
Msa0145450	MtrunA17_Chr1g0154171	66.488	373	63	4	5	333	6	360	3.63e-166	467
Msa0145450	MtrunA17_Chr1g0154161	67.273	330	91	3	5	334	13	325	1.20e-158	446
Msa0145450	MtrunA17_Chr1g0154131	58.876	338	95	6	6	326	168	478	6.34e-130	379
Msa0145450	MtrunA17_Chr1g0154131	45.016	311	123	5	4	314	8	270	2.36e-77	244
Msa0145450	MtrunA17_Chr1g0154211	50.739	406	109	7	2	328	3	396	3.09e-129	375
Msa0145450	MtrunA17_Chr1g0154111	54.400	375	103	5	4	325	8	367	3.71e-128	379
Msa0145450	MtrunA17_Chr1g0154111	55.429	175	62	4	37	197	368	540	8.55e-47	167
Msa0145450	MtrunA17_Chr1g0154151	57.658	333	70	4	37	326	19	323	7.93e-124	358
Msa0145450	MtrunA17_Chr1g0154071	50.964	363	112	6	10	322	145	491	2.15e-112	335

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0145450	AT4G33280.1	34.675	323	177	8	8	315	11	314	1.19e-48	166
Msa0145450	AT4G33280.2	53.788	132	58	2	8	138	11	140	1.43e-43	152
Msa0145450	AT4G33280.3	53.788	132	58	2	8	138	11	140	1.26e-42	152
Msa0145450	AT4G34400.2	37.273	110	63	3	15	121	8	114	7.91e-18	84.0
Msa0145450	AT4G34400.1	37.273	110	63	3	15	121	8	114	7.91e-18	84.0
Msa0145450	AT3G18990.2	23.547	344	193	10	22	312	6	332	1.94e-17	82.8
Msa0145450	AT3G18990.1	23.547	344	193	10	22	312	6	332	2.00e-17	82.4
Msa0145450	AT5G66980.1	22.848	302	180	9	34	305	21	299	2.52e-14	73.2
Msa0145450	AT5G60130.5	41.573	89	47	2	36	119	27	115	6.35e-14	70.9
Msa0145450	AT3G06160.1	27.168	173	117	4	22	188	26	195	6.45e-14	72.0

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Find 82 sgRNAs with CRISPR-Local

Find 233 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGAGGAGGTAAATGATT+GGG	0.509472	1_4:-5138419	None:intergenic
AAACCAACACATATGCTAAA+CGG	0.480555	1_4:+5138771	Msa0145450:CDS
AAACCCTTCAACAGTTCACG+AGG	0.670720	1_4:-5136132	None:intergenic
AAAGGTACAAAGCTAAAGTA+GGG	0.563924	1_4:-5136159	None:intergenic
AACAAAATATGGAAAAGAGG+AGG	0.585641	1_4:-5138431	None:intergenic
AACTCCACTAGGACCTTTAA+GGG	0.479312	1_4:-5137361	None:intergenic
AACTCTATCCCATATGCTAA+AGG	0.447966	1_4:+5138737	Msa0145450:CDS
AAGGTCCTAGTGGAGTTGTG+TGG	0.624686	1_4:+5137367	Msa0145450:CDS
AAGGTCTTGACCAGCAAAAT+GGG	0.444958	1_4:+5138927	Msa0145450:CDS
AAGTTGTTGTTGAAAATCGG+TGG	0.662291	1_4:-5136307	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TGAAATGGATGAACTGAAAA+TGG	-	chr1_4:5136269-5136288	None:intergenic	30.0%
TATGAAGAAATTGGGTGAAA+TGG	-	chr1_4:5136284-5136303	None:intergenic	30.0%
CTAATATGAACACTTCAGAA+GGG	-	chr1_4:5136388-5136407	None:intergenic	30.0%
TGGGCAAGTTAAAATATTCA+AGG	-	chr1_4:5136455-5136474	None:intergenic	30.0%
ATTTACACAGATAGTGTTTG+CGG	-	chr1_4:5136809-5136828	None:intergenic	30.0%
CATTAATGACCTATGAAGTA+CGG	+	chr1_4:5136872-5136891	Msa0145450:intron	30.0%
ACTATATTACAACTGTGTGA+GGG	+	chr1_4:5137061-5137080	Msa0145450:intron	30.0%
AATTTGAACTTTGTACCTTG+AGG	+	chr1_4:5137084-5137103	Msa0145450:intron	30.0%
ACATGATTTACATACACTTG+AGG	-	chr1_4:5137143-5137162	None:intergenic	30.0%
GATACTGTTTACTTCACAAA+CGG	+	chr1_4:5137414-5137433	Msa0145450:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_4	gene	5136093	5139188	5136093	ID=Msa0145450;Name=Msa0145450
chr1_4	mRNA	5136093	5139188	5136093	ID=Msa0145450-mRNA-1;Parent=Msa0145450;Name=Msa0145450-mRNA-1;_AED=0.40;_eAED=0.40;_QI=129\|0.66\|0.75\|1\|1\|1\|4\|215\|334
chr1_4	exon	5136093	5136329	5136093	ID=Msa0145450-mRNA-1:exon:2122;Parent=Msa0145450-mRNA-1
chr1_4	exon	5137306	5137750	5137306	ID=Msa0145450-mRNA-1:exon:2123;Parent=Msa0145450-mRNA-1
chr1_4	exon	5138371	5138495	5138371	ID=Msa0145450-mRNA-1:exon:2124;Parent=Msa0145450-mRNA-1
chr1_4	exon	5138647	5139188	5138647	ID=Msa0145450-mRNA-1:exon:2125;Parent=Msa0145450-mRNA-1
chr1_4	five_prime_UTR	5136093	5136221	5136093	ID=Msa0145450-mRNA-1:five_prime_utr;Parent=Msa0145450-mRNA-1
chr1_4	CDS	5136222	5136329	5136222	ID=Msa0145450-mRNA-1:cds;Parent=Msa0145450-mRNA-1
chr1_4	CDS	5137306	5137750	5137306	ID=Msa0145450-mRNA-1:cds;Parent=Msa0145450-mRNA-1
chr1_4	CDS	5138371	5138495	5138371	ID=Msa0145450-mRNA-1:cds;Parent=Msa0145450-mRNA-1
chr1_4	CDS	5138647	5138973	5138647	ID=Msa0145450-mRNA-1:cds;Parent=Msa0145450-mRNA-1
chr1_4	three_prime_UTR	5138974	5139188	5138974	ID=Msa0145450-mRNA-1:three_prime_utr;Parent=Msa0145450-mRNA-1

Gene Sequence

>Msa0145450

ATGGTAGGAAGCACCAAATCTTGGGAGGAAGAGATTTACTGGAACCATTTTCAGTTCATCCATTTCACCCAATTTCTTCATAGCACCACCGATTTTCAACAACAACTTGCACTTCCTAAAACATTTTCAGACAATTTAAAGAAGAAGTTGCCAGAAAATGTGACCCTTAAAGGTCCTAGTGGAGTTGTGTGGAATATAGAGCTGACAACTGAAGATGATACTGTTTACTTCACAAACGGTTGGCAACGATTTGTGAATGATCACTCTTTGAAAGAGAGTGATTTCCTTTTCTTCAAGTACAATGGCGAATCGTTGTTTGAAGTTTTAATCTTTGATGGACTTAGTTTCTGTGAGAAGGCAGCTTCTTATTTTGTTGGAAAATGTGGTCATGCTCAAACCGAACAAGGGGGTATCAAGGCCAAGGACACAAACAAGTCTGTGGAAGAAATCAATACTACTTTTGATGCGGGTGTGGAGTCTGCTTCCCCTGAGAAGTGCATGGCTGATGCAGTCACTAAGACCACCCCTGTTGCAGCTCCCTCCCAATTAACTGAGGCATCCATCGTGCATAGAAGTGGGAAGGAAGATGTGAAAAAGATAGCCCAATCATTTACCTCCTCTTTTCCATATTTTGTTAAAATATTTAAAACCGGCGATATCAATGTTTCACGTACTATGAAAATTCCATATCATTTCTCTGCTGCACATCTCCCTGACTGCAAAACAGAAGTTACGCTTCGCAATTCAAGAGGCGAATGTTGGACTGTGAACTCTATCCCATATGCTAAAGGGAGAACAGTTCAAACCAACACATATGCTAAACGGAGACCAGTTCACACCTTTTGTGGGGGTTGGGTGGCCTTTGTTCGTGATAATAACATTAAATTTGGTGACACGTGCATTTTTGAGCTCGTTTCTGATTATGTGATGCAAGTTCATATATCTGGGGTTGGAAAGGAAGGTCTTGACCAGCAAAATGGGAATGGGAAACTTAATAGCATGTAG

Protein sequence

>Msa0145450

MVGSTKSWEEEIYWNHFQFIHFTQFLHSTTDFQQQLALPKTFSDNLKKKLPENVTLKGPSGVVWNIELTTEDDTVYFTNGWQRFVNDHSLKESDFLFFKYNGESLFEVLIFDGLSFCEKAASYFVGKCGHAQTEQGGIKAKDTNKSVEEINTTFDAGVESASPEKCMADAVTKTTPVAAPSQLTEASIVHRSGKEDVKKIAQSFTSSFPYFVKIFKTGDINVSRTMKIPYHFSAAHLPDCKTEVTLRNSRGECWTVNSIPYAKGRTVQTNTYAKRRPVHTFCGGWVAFVRDNNIKFGDTCIFELVSDYVMQVHISGVGKEGLDQQNGNGKLNSM*