AlfalfaGEDB | Msa0114110

M. sativa cultivar zhongmu-4 / Msa0114110

KEGG: -
GO: -
PFAM: PF00612.30

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0114110	XP_013467475.1	96.486	370	13	0	1	370	1	370	0.0	695

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0114110	sp\|A0A1P8BH03\|IQD12_ARATH	44.837	368	187	7	1	357	1	363	6.47e-70	227

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0114110	A0A072VI83	96.486	370	13	0	1	370	1	370	0.0	695

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0114110	Msa1024740	0.804275	2.335845e-49	-8.615850e-47
Msa0114110	Msa1152510	0.811514	6.738410e-51	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0114110	MtrunA17_Chr1g0172611	96.486	370	13	0	1	370	1	370	0.0	695
Msa0114110	MtrunA17_Chr4g0020671	33.420	386	218	11	3	367	4	371	8.27e-47	165
Msa0114110	MtrunA17_Chr7g0273861	32.824	393	210	13	1	354	1	378	5.22e-29	116
Msa0114110	MtrunA17_Chr6g0462191	32.303	356	162	10	50	346	56	391	5.76e-23	99.4
Msa0114110	MtrunA17_Chr1g0200161	34.014	147	87	3	98	241	134	273	7.27e-19	87.8
Msa0114110	MtrunA17_Chr3g0126901	29.008	393	223	13	3	360	4	375	2.52e-16	80.1
Msa0114110	MtrunA17_Chr4g0045641	34.752	141	81	3	106	240	147	282	3.33e-16	80.1
Msa0114110	MtrunA17_Chr1g0203711	30.657	411	223	13	8	368	8	406	5.24e-16	79.0
Msa0114110	MtrunA17_Chr1g0181581	35.766	137	80	1	100	236	111	239	6.33e-16	79.0
Msa0114110	MtrunA17_Chr1g0204901	46.939	147	61	4	16	151	42	182	1.91e-15	77.4

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0114110	AT5G03960.3	44.837	368	187	7	1	357	1	363	6.58e-71	227
Msa0114110	AT5G03960.2	44.837	368	187	7	1	357	1	363	6.58e-71	227
Msa0114110	AT5G03960.1	44.687	367	188	7	1	357	1	362	3.00e-70	226
Msa0114110	AT5G13460.1	32.353	374	208	12	1	352	1	351	1.58e-38	143
Msa0114110	AT5G03040.1	25.172	437	236	13	1	360	1	423	7.10e-25	105
Msa0114110	AT5G03040.3	25.172	437	236	13	1	360	1	423	7.10e-25	105
Msa0114110	AT5G03040.2	25.172	437	236	13	1	360	1	423	7.10e-25	105
Msa0114110	AT3G52290.1	38.800	250	117	8	5	238	3	232	1.41e-21	95.9
Msa0114110	AT4G10640.1	28.713	404	198	18	1	352	1	366	2.02e-21	95.5
Msa0114110	AT3G09710.1	27.817	284	173	9	1	271	1	265	1.10e-16	81.6

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Find 67 sgRNAs with CRISPR-Local

Find 123 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATCATCTCAAGGGTGTA+GGG	0.510041	1_3:-30516229	Msa0114110:three_prime_UTR
AAAATCTTCACATGCATATA+TGG	0.261916	1_3:-30516159	Msa0114110:three_prime_UTR
AAAGCATTAAGAGCATTGAA+GGG	0.611416	1_3:-30517287	Msa0114110:CDS
AACAGCTTCACCGCGAATTA+TGG	0.256063	1_3:+30517247	None:intergenic
AAGAAGAGTATTGCCTCATG+CGG	0.677390	1_3:+30516763	None:intergenic
AATCTAGCAAAGCTAAGGTA+AGG	0.545302	1_3:-30516331	Msa0114110:CDS
AATGTGGGTGGAGATTTGCA+AGG	0.633509	1_3:-30518099	Msa0114110:CDS
AATTCCAAGTTCTTTGACTA+TGG	0.222640	1_3:+30516856	None:intergenic
AATTTCTAAAATCATCTCAA+GGG	0.608844	1_3:-30516236	Msa0114110:exon
ACATAAAGAAACGTGTAACT+GGG	0.593317	1_3:-30516534	Msa0114110:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TGGTATGTGAAAAATCTTGA+TGG	-	chr1_3:30516011-30516030	Msa0114110:three_prime_UTR	30.0%
TCTATTCATTTCTGAGTCAA+AGG	-	chr1_3:30516068-30516087	Msa0114110:three_prime_UTR	30.0%
ATCTAGAAGCAAAAGAAATG+TGG	-	chr1_3:30516210-30516229	Msa0114110:three_prime_UTR	30.0%
TCTAGAAGCAAAAGAAATGT+GGG	-	chr1_3:30516211-30516230	Msa0114110:three_prime_UTR	30.0%
TTCTTATTCGTATCTATCCA+AGG	-	chr1_3:30516431-30516450	Msa0114110:CDS	30.0%
TCTTATTCGTATCTATCCAA+GGG	-	chr1_3:30516432-30516451	Msa0114110:CDS	30.0%
ATTCTCAGAGTGAAATAGAA+AGG	+	chr1_3:30516852-30516871	None:intergenic	30.0%
AAAAACTCAGAAGGAAATTC+AGG	-	chr1_3:30517145-30517164	Msa0114110:CDS	30.0%
GATAGAAGAGAATGAACTTA+AGG	-	chr1_3:30517241-30517260	Msa0114110:CDS	30.0%
AATTCCAAGTTCTTTGACTA+TGG	+	chr1_3:30517472-30517491	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_3	gene	30515945	30518402	30515945	ID=Msa0114110;Name=Msa0114110
chr1_3	mRNA	30515945	30518402	30515945	ID=Msa0114110-mRNA-1;Parent=Msa0114110;Name=Msa0114110-mRNA-1;_AED=0.05;_eAED=0.05;_QI=85\|1\|1\|1\|1\|1\|5\|305\|370
chr1_3	exon	30515945	30516642	30515945	ID=Msa0114110-mRNA-1:exon:10129;Parent=Msa0114110-mRNA-1
chr1_3	exon	30516755	30516886	30516755	ID=Msa0114110-mRNA-1:exon:10128;Parent=Msa0114110-mRNA-1
chr1_3	exon	30517085	30517315	30517085	ID=Msa0114110-mRNA-1:exon:10127;Parent=Msa0114110-mRNA-1
chr1_3	exon	30517841	30518131	30517841	ID=Msa0114110-mRNA-1:exon:10126;Parent=Msa0114110-mRNA-1
chr1_3	exon	30518252	30518402	30518252	ID=Msa0114110-mRNA-1:exon:10125;Parent=Msa0114110-mRNA-1
chr1_3	five_prime_UTR	30518318	30518402	30518318	ID=Msa0114110-mRNA-1:five_prime_utr;Parent=Msa0114110-mRNA-1
chr1_3	CDS	30518252	30518317	30518252	ID=Msa0114110-mRNA-1:cds;Parent=Msa0114110-mRNA-1
chr1_3	CDS	30517841	30518131	30517841	ID=Msa0114110-mRNA-1:cds;Parent=Msa0114110-mRNA-1
chr1_3	CDS	30517085	30517315	30517085	ID=Msa0114110-mRNA-1:cds;Parent=Msa0114110-mRNA-1
chr1_3	CDS	30516755	30516886	30516755	ID=Msa0114110-mRNA-1:cds;Parent=Msa0114110-mRNA-1
chr1_3	CDS	30516250	30516642	30516250	ID=Msa0114110-mRNA-1:cds;Parent=Msa0114110-mRNA-1
chr1_3	three_prime_UTR	30515945	30516249	30515945	ID=Msa0114110-mRNA-1:three_prime_utr;Parent=Msa0114110-mRNA-1

Gene Sequence

>Msa0114110

ATGGCAAAGAGGAAGAGCTGGTTTGGATGGGTGAAAAGTCTATTCATTTCTGAGTCAAAGGACAAAAAGCAAAAGAAATGTGGGTGGAGATTTGCAAGGATTAAACAAAAGCTATTATACCCCAAAATTACAGCTTCAAACAGAACATTGATTGAAGCAAGTGAAGAACAGAGAAAGCATGCGTTGGCTGTGGCTATTGCAACAGCATCTGCTGCAGAGGCTGCCATTGCTGCTGCACATGCTGCTGCTGAGGTTGTCAAGCTCACAGGTGGCGCCTCTCGTTCTTATTCGTATCTATCCAAGGGAGACAGAAGTTTGGCTGCCATCAAGATTCAAAGTGTCTACCGTGCGCATCTCGCTAGGAAAGCATTAAGAGCATTGAAGGGAGTGATAAGACTTCAAGCCATAATTCGCGGTGAAGCTGTTAGGCGTCAAGTGTCCAGAACTTTGAAGAATATTCCCTCAAATGCAAAAACTCAGAAGGAAATTCAGGAAAGAAGCATCCATACGAAGGAAGAAAACTACAAAAATGACCATATAAAACAGTTCCCAAAGCAAAAGAAAAAGATAGAAGAGAATGAACTTAAGCCTGAGCTCCATAGTCAAAGAACTTGGAATTGTAGCTCACACTCAAGAGAAGACATTGAAGCCATATGGTTAAGAAAGCAAGAGGCTATTATCAAAAGAGACCGCATGAGGCAATACTCTTCTTCGCAAAAGGAGAGAATGTTTTCCCAAATGGTGGAAGAATCTTTGCACAACAAAGAATTTGGTGAAGATAATTGTCGTACTCTAGGAGAGTGGCTACATAAAGAAACGTGTAACTGGGATGTTCTTTACAAACCTGCTCATCCTTCAAATATCACAATAAGAAATGAAGTGCAAGAAGGATTGAAATCACAAGTTTCAGTCCAAAGAAAATCATTTTCTCATGTAAAAAGAAGTTCAATCGGAGACGAGAGTTCCATGCCAAATTCTCCGGTTTTTCCTAAGTACATGGCTGTGACTGAATCTAGCAAAGCTAAGGTAAGGTCTATGAGCACACCAAGGCTAAGAACAGGATTTTCGGATGTGTGTTCAAATCAAAATGAGCACCAATGCATGAATTTCTAA

Protein sequence

>Msa0114110

MAKRKSWFGWVKSLFISESKDKKQKKCGWRFARIKQKLLYPKITASNRTLIEASEEQRKHALAVAIATASAAEAAIAAAHAAAEVVKLTGGASRSYSYLSKGDRSLAAIKIQSVYRAHLARKALRALKGVIRLQAIIRGEAVRRQVSRTLKNIPSNAKTQKEIQERSIHTKEENYKNDHIKQFPKQKKKIEENELKPELHSQRTWNCSSHSREDIEAIWLRKQEAIIKRDRMRQYSSSQKERMFSQMVEESLHNKEFGEDNCRTLGEWLHKETCNWDVLYKPAHPSNITIRNEVQEGLKSQVSVQRKSFSHVKRSSIGDESSMPNSPVFPKYMAVTESSKAKVRSMSTPRLRTGFSDVCSNQNEHQCMNF*