AlfalfaGEDB | Msa0114930

M. sativa cultivar zhongmu-4 / Msa0114930

KEGG: K11699
GO: GO:0001101 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009605 GO:0009607 GO:0009615 GO:0009719 GO:0009725 GO:0009751 GO:0009892 GO:0009987 GO:0010025 GO:0010033 GO:0010166 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0019222 GO:0030422 GO:0031047 GO:0031050 GO:0034641 GO:0035194 GO:0040029 GO:0042221 GO:0042493 GO:0043170 GO:0043207 GO:0043331 GO:0044237 GO:0044238 GO:0046483 GO:0046677 GO:0048518 GO:0048519 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0060147 GO:0060148 GO:0060255 GO:0060968 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:1901360 GO:1901568 GO:1901570 GO:1901576 GO:1901698 GO:1901699 GO:1901700
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0114930	sp\|Q9LQV2\|RDR1_ARATH	40.426	188	97	3	4	191	2	174	1.50e-40	149

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0114930	A0A072VF31	67.539	191	47	2	1	191	1	176	1.54e-74	249

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0114910	Msa0114930	0.945724	1.786259e-104	-8.615850e-47
Msa0114920	Msa0114930	0.951505	1.772437e-109	-8.615850e-47
Msa0114930	Msa0114940	0.949928	4.701505e-108	-8.615850e-47
Msa0114930	Msa0114950	0.912265	2.373543e-83	-8.615850e-47
Msa0114930	Msa0114970	0.922401	1.032226e-88	-8.615850e-47
Msa0114930	Msa0114980	0.876595	1.232365e-68	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0114930	MtrunA17_Chr1g0161491	67.539	191	47	2	1	191	15	190	1.43e-76	248
Msa0114930	MtrunA17_Chr1g0161471	65.285	193	51	2	1	191	1	179	2.62e-76	248
Msa0114930	MtrunA17_Chr1g0161511	62.887	194	54	4	1	191	1	179	6.84e-69	227
Msa0114930	MtrunA17_Chr1g0161521	62.887	194	54	4	1	191	1	179	1.20e-68	226
Msa0114930	MtrunA17_Chr1g0161451	33.871	186	108	3	5	190	3	173	3.86e-27	107

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0114930	AT1G14790.1	40.426	188	97	3	4	191	2	174	1.52e-41	149

Find 26 sgRNAs with CRISPR-Local

Find 34 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GACGATGTTAAATTGTATTT+TGG	0.150856	1_3:+33314064	Msa0114930:CDS
AGCTTTCATATGAGAATATT+TGG	0.191630	1_3:+33314212	Msa0114930:CDS
ATGGATGCTAGTGCAGATTT+TGG	0.192772	1_3:+33314130	Msa0114930:CDS
CTATTTAAAAGTTACGAAAA+TGG	0.261211	1_3:+33313997	Msa0114930:CDS
TGCAAAGTATCTATTGATTC+AGG	0.268018	1_3:+33314267	Msa0114930:CDS
TAGAAGAATGCCGTTGGCTT+TGG	0.337561	1_3:-33314034	None:intergenic
CTCTGTATTGTGGGGAAAGA+TGG	0.454381	1_3:+33314111	Msa0114930:CDS
TTGGGATTGGATTGAGAAAG+TGG	0.455481	1_3:+33314149	Msa0114930:CDS
ACATTTGTAGAGCAGTATAC+TGG	0.461229	1_3:+33313788	Msa0114930:CDS
GGAAAGATTCTCTGTATTGT+GGG	0.467936	1_3:+33314102	Msa0114930:CDS
TCTACAAATGTCTTTACATC+AGG	0.480962	1_3:-33313776	None:intergenic
AGTAAAACAATTAAGTTGCA+TGG	0.485965	1_3:+33313731	Msa0114930:CDS
AGGAAAGATTCTCTGTATTG+TGG	0.507749	1_3:+33314101	Msa0114930:CDS
AGATCATGCTACATATATGA+TGG	0.530126	1_3:+33313937	Msa0114930:CDS
GTTTGAACTTCTTATCGTCA+TGG	0.543349	1_3:-33314186	None:intergenic
GAGATTTGTTCAAATTCGAA+CGG	0.545671	1_3:+33313886	Msa0114930:CDS
TCAGGCACGGTGACATGATG+AGG	0.567959	1_3:-33313758	None:intergenic
AGATTGAACTGCATCAACCA+TGG	0.634369	1_3:+33314236	Msa0114930:CDS
CAGGCACGGTGACATGATGA+GGG	0.635211	1_3:-33313757	None:intergenic
AATCTGTAGAAGAATGCCGT+TGG	0.636903	1_3:-33314040	None:intergenic
AGATATTGATCCAAAGCCAA+CGG	0.640968	1_3:+33314024	Msa0114930:CDS
GAAAGATTCTCTGTATTGTG+GGG	0.645886	1_3:+33314103	Msa0114930:CDS
AAATGTCTTTACATCAGGCA+CGG	0.659012	1_3:-33313771	None:intergenic
TATATGATGGCCTTATGTAG+CGG	0.659739	1_3:+33313950	Msa0114930:CDS
ATATGTAGCATGATCTGTAG+TGG	0.696738	1_3:-33313930	None:intergenic
GTATACTGGTGAAGAAAGTG+TGG	0.711055	1_3:+33313802	Msa0114930:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	CATTTTCGTAACTTTTAAAT+AGG	-	chr1_3:33313999-33314018	None:intergenic	20.0%
!!	CTATTTAAAAGTTACGAAAA+TGG	+	chr1_3:33313997-33314016	Msa0114930:CDS	20.0%
!!	AGTAAAACAATTAAGTTGCA+TGG	+	chr1_3:33313731-33313750	Msa0114930:CDS	25.0%
!!	GACGATGTTAAATTGTATTT+TGG	+	chr1_3:33314064-33314083	Msa0114930:CDS	25.0%
!	AGCTTTCATATGAGAATATT+TGG	+	chr1_3:33314212-33314231	Msa0114930:CDS	25.0%
	TCTACAAATGTCTTTACATC+AGG	-	chr1_3:33313779-33313798	None:intergenic	30.0%
	GTGTGGTTGCTATAAAAATA+AGG	+	chr1_3:33313819-33313838	Msa0114930:CDS	30.0%
!	GAGATTTGTTCAAATTCGAA+CGG	+	chr1_3:33313886-33313905	Msa0114930:CDS	30.0%
	AGATCATGCTACATATATGA+TGG	+	chr1_3:33313937-33313956	Msa0114930:CDS	30.0%
!!!	CAATTTTTTTCCGCTACATA+AGG	-	chr1_3:33313963-33313982	None:intergenic	30.0%
!!!	TACTTTGCAGTTTTAAACCA+TGG	-	chr1_3:33314256-33314275	None:intergenic	30.0%
!!	TGCAAAGTATCTATTGATTC+AGG	+	chr1_3:33314267-33314286	Msa0114930:CDS	30.0%
	AAATGTCTTTACATCAGGCA+CGG	-	chr1_3:33313774-33313793	None:intergenic	35.0%
	ACATTTGTAGAGCAGTATAC+TGG	+	chr1_3:33313788-33313807	Msa0114930:CDS	35.0%
	ATATGTAGCATGATCTGTAG+TGG	-	chr1_3:33313933-33313952	None:intergenic	35.0%
!!	TATATGATGGCCTTATGTAG+CGG	+	chr1_3:33313950-33313969	Msa0114930:CDS	35.0%
	AGATATTGATCCAAAGCCAA+CGG	+	chr1_3:33314024-33314043	Msa0114930:CDS	35.0%
	AGGAAAGATTCTCTGTATTG+TGG	+	chr1_3:33314101-33314120	Msa0114930:CDS	35.0%
	GGAAAGATTCTCTGTATTGT+GGG	+	chr1_3:33314102-33314121	Msa0114930:CDS	35.0%
	GAAAGATTCTCTGTATTGTG+GGG	+	chr1_3:33314103-33314122	Msa0114930:CDS	35.0%
	GTTTGAACTTCTTATCGTCA+TGG	-	chr1_3:33314189-33314208	None:intergenic	35.0%
	GTATACTGGTGAAGAAAGTG+TGG	+	chr1_3:33313802-33313821	Msa0114930:CDS	40.0%
	GCGGAAAAAAATTGCAGTTC+TGG	+	chr1_3:33313969-33313988	Msa0114930:CDS	40.0%
	AATCTGTAGAAGAATGCCGT+TGG	-	chr1_3:33314043-33314062	None:intergenic	40.0%
!!	TTTTGGCTGTCAGATCTCAA+AGG	+	chr1_3:33314081-33314100	Msa0114930:CDS	40.0%
!	ATGGATGCTAGTGCAGATTT+TGG	+	chr1_3:33314130-33314149	Msa0114930:CDS	40.0%
!	TGGATGCTAGTGCAGATTTT+GGG	+	chr1_3:33314131-33314150	Msa0114930:CDS	40.0%
	TTGGGATTGGATTGAGAAAG+TGG	+	chr1_3:33314149-33314168	Msa0114930:CDS	40.0%
	AGATTGAACTGCATCAACCA+TGG	+	chr1_3:33314236-33314255	Msa0114930:CDS	40.0%
!!	TAGAAGAATGCCGTTGGCTT+TGG	-	chr1_3:33314037-33314056	None:intergenic	45.0%
	CTCTGTATTGTGGGGAAAGA+TGG	+	chr1_3:33314111-33314130	Msa0114930:CDS	45.0%
!!!	GCTAGTGCAGATTTTGGGAT+TGG	+	chr1_3:33314136-33314155	Msa0114930:CDS	45.0%
	CAGGCACGGTGACATGATGA+GGG	-	chr1_3:33313760-33313779	None:intergenic	55.0%
	TCAGGCACGGTGACATGATG+AGG	-	chr1_3:33313761-33313780	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr1_3	gene	33313722	33314318	33313722	ID=Msa0114930;Name=Msa0114930
chr1_3	mRNA	33313722	33314318	33313722	ID=Msa0114930-mRNA-1;Parent=Msa0114930;Name=Msa0114930-mRNA-1;_AED=0.13;_eAED=0.13;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|198
chr1_3	exon	33313722	33314318	33313722	ID=Msa0114930-mRNA-1:exon:10472;Parent=Msa0114930-mRNA-1
chr1_3	CDS	33313722	33314318	33313722	ID=Msa0114930-mRNA-1:cds;Parent=Msa0114930-mRNA-1

Gene Sequence

>Msa0114930

ATGGTTAAAAGTAAAACAATTAAGTTGCATGGATTCCCTCATCATGTCACCGTGCCTGATGTAAAGACATTTGTAGAGCAGTATACTGGTGAAGAAAGTGTGGTTGCTATAAAAATAAGGAAGTGTAGTTGCTATAAAAATAAGTGTTGTTGCTATAAAAAGAAGAGATTTGTTCAAATTCGAACGGCATTTGCAATTATTCAATTCACCACTACAGATCATGCTACATATATGATGGCCTTATGTAGCGGAAAAAAATTGCAGTTCTGGAAATCCTATTTAAAAGTTACGAAAATGGAAAGAGATATTGATCCAAAGCCAACGGCATTCTTCTACAGATTAGACGATGTTAAATTGTATTTTGGCTGTCAGATCTCAAAGGAAAGATTCTCTGTATTGTGGGGAAAGATGGATGCTAGTGCAGATTTTGGGATTGGATTGAGAAAGTGGCGATTCTCTATGTGCCATGACGATAAGAAGTTCAAACTTGAGCTTTCATATGAGAATATTTGGAAGATTGAACTGCATCAACCATGGTTTAAAACTGCAAAGTATCTATTGATTCAGGTTGTTAAATTCAACTTTTCATTATTTTAA

Protein sequence

>Msa0114930

MVKSKTIKLHGFPHHVTVPDVKTFVEQYTGEESVVAIKIRKCSCYKNKCCCYKKKRFVQIRTAFAIIQFTTTDHATYMMALCSGKKLQFWKSYLKVTKMERDIDPKPTAFFYRLDDVKLYFGCQISKERFSVLWGKMDASADFGIGLRKWRFSMCHDDKKFKLELSYENIWKIELHQPWFKTAKYLLIQVVKFNFSLF*