AlfalfaGEDB | Msa0114920

M. sativa cultivar zhongmu-4 / Msa0114920

KEGG: K11699
GO: GO:0001101 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009605 GO:0009607 GO:0009615 GO:0009719 GO:0009725 GO:0009751 GO:0009892 GO:0009987 GO:0010025 GO:0010033 GO:0010166 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0019222 GO:0030422 GO:0031047 GO:0031050 GO:0034641 GO:0035194 GO:0040029 GO:0042221 GO:0042493 GO:0043170 GO:0043207 GO:0043331 GO:0044237 GO:0044238 GO:0046483 GO:0046677 GO:0048518 GO:0048519 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0060147 GO:0060148 GO:0060255 GO:0060968 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:1901360 GO:1901568 GO:1901570 GO:1901576 GO:1901698 GO:1901699 GO:1901700
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0114920	KEH40644.1	67.539	191	47	2	1	191	1	176	3.22e-74	249

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0114920	sp\|Q9LQV2\|RDR1_ARATH	40.426	188	97	3	4	191	2	174	1.50e-40	149

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0114920	A0A072VF31	67.539	191	47	2	1	191	1	176	1.54e-74	249

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0094130	Msa0114920	0.806787	6.941939e-50	-8.615850e-47
Msa0114910	Msa0114920	0.965438	1.323526e-124	-8.615850e-47
Msa0114920	Msa0114930	0.951505	1.772437e-109	-8.615850e-47
Msa0114920	Msa0114940	0.945381	3.399259e-104	-8.615850e-47
Msa0114920	Msa0114950	0.911603	5.046575e-83	-8.615850e-47
Msa0114920	Msa0114970	0.934410	4.215912e-96	-8.615850e-47
Msa0114920	Msa0114980	0.882757	7.972062e-71	-8.615850e-47
Msa0114920	Msa0142480	0.817921	2.565376e-52	-8.615850e-47
Msa0114920	Msa0146260	0.804634	1.966558e-49	-8.615850e-47
Msa0114920	Msa0237310	0.801320	9.521182e-49	-8.615850e-47
Msa0114920	Msa0330340	0.804357	2.245896e-49	-8.615850e-47
Msa0114920	Msa0336030	0.801971	6.998088e-49	-8.615850e-47
Msa0114920	Msa0376350	0.809958	1.462572e-50	-8.615850e-47
Msa0114920	Msa0388960	0.800244	1.578637e-48	-8.615850e-47
Msa0114920	Msa0410500	0.802929	4.443134e-49	-8.615850e-47
Msa0114920	Msa0439970	0.806562	7.743649e-50	-8.615850e-47
Msa0114920	Msa0467430	0.810451	1.145367e-50	-8.615850e-47
Msa0114920	Msa0488560	0.800311	1.529927e-48	-8.615850e-47
Msa0114920	Msa0503060	0.813060	3.099078e-51	-8.615850e-47
Msa0114920	Msa0516170	0.804665	1.937128e-49	-8.615850e-47
Msa0114920	Msa0657750	0.812727	3.665969e-51	-8.615850e-47
Msa0114920	Msa0693380	0.800028	1.746217e-48	-8.615850e-47
Msa0114920	Msa0828920	0.806745	7.086153e-50	-8.615850e-47
Msa0114920	Msa0908050	0.822955	1.796027e-53	-8.615850e-47
Msa0114920	Msa0933230	0.801003	1.105431e-48	-8.615850e-47
Msa0114920	Msa1041670	0.800132	1.663217e-48	-8.615850e-47
Msa0114920	Msa1063950	0.801270	9.745069e-49	-8.615850e-47
Msa0114920	Msa1108160	0.810062	1.389237e-50	-8.615850e-47
Msa0114920	Msa1166560	0.801215	1.000128e-48	-8.615850e-47
Msa0114920	Msa1179970	0.801971	6.997965e-49	-8.615850e-47
Msa0114920	Msa1199570	0.802958	4.382625e-49	-8.615850e-47
Msa0114920	Msa1224830	0.807228	5.599219e-50	-8.615850e-47
Msa0114920	Msa1297290	0.812804	3.526677e-51	-8.615850e-47
Msa0114920	Msa1313150	0.801419	9.084715e-49	-8.615850e-47
Msa0114920	Msa1328710	0.801889	7.274658e-49	-8.615850e-47
Msa0114920	Msa1374040	0.801911	7.200125e-49	-8.615850e-47
Msa0114920	Msa1385000	0.811383	7.195572e-51	-8.615850e-47
Msa0114920	Msa1402400	0.801502	8.735846e-49	-8.615850e-47
Msa0114920	Msa1423880	0.804871	1.754265e-49	-8.615850e-47
Msa0114920	Msa1451670	0.801373	9.282734e-49	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0114920	MtrunA17_Chr1g0161491	67.539	191	47	2	1	191	15	190	1.43e-76	248
Msa0114920	MtrunA17_Chr1g0161471	65.285	193	51	2	1	191	1	179	2.62e-76	248
Msa0114920	MtrunA17_Chr1g0161511	62.887	194	54	4	1	191	1	179	6.84e-69	227
Msa0114920	MtrunA17_Chr1g0161521	62.887	194	54	4	1	191	1	179	1.20e-68	226
Msa0114920	MtrunA17_Chr1g0161451	33.871	186	108	3	5	190	3	173	3.86e-27	107

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0114920	AT1G14790.1	40.426	188	97	3	4	191	2	174	1.52e-41	149

Find 26 sgRNAs with CRISPR-Local

Find 34 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GACGATGTTAAATTGTATTT+TGG	0.150856	1_3:+33294133	Msa0114920:CDS
AGCTTTCATATGAGAATATT+TGG	0.191630	1_3:+33294281	Msa0114920:CDS
ATGGATGCTAGTGCAGATTT+TGG	0.192772	1_3:+33294199	Msa0114920:CDS
CTATTTAAAAGTTACGAAAA+TGG	0.261211	1_3:+33294066	Msa0114920:CDS
TGCAAAGTATCTATTGATTC+AGG	0.268018	1_3:+33294336	Msa0114920:CDS
TAGAAGAATGCCGTTGGCTT+TGG	0.337561	1_3:-33294103	None:intergenic
CTCTGTATTGTGGGGAAAGA+TGG	0.454381	1_3:+33294180	Msa0114920:CDS
TTGGGATTGGATTGAGAAAG+TGG	0.455481	1_3:+33294218	Msa0114920:CDS
ACATTTGTAGAGCAGTATAC+TGG	0.461229	1_3:+33293857	Msa0114920:CDS
GGAAAGATTCTCTGTATTGT+GGG	0.467936	1_3:+33294171	Msa0114920:CDS
TCTACAAATGTCTTTACATC+AGG	0.480962	1_3:-33293845	None:intergenic
AGTAAAACAATTAAGTTGCA+TGG	0.485965	1_3:+33293800	Msa0114920:CDS
AGGAAAGATTCTCTGTATTG+TGG	0.507749	1_3:+33294170	Msa0114920:CDS
AGATCATGCTACATATATGA+TGG	0.530126	1_3:+33294006	Msa0114920:CDS
GTTTGAACTTCTTATCGTCA+TGG	0.543349	1_3:-33294255	None:intergenic
GAGATTTGTTCAAATTCGAA+CGG	0.545671	1_3:+33293955	Msa0114920:CDS
TCAGGCACGGTGACATGATG+AGG	0.567959	1_3:-33293827	None:intergenic
AGATTGAACTGCATCAACCA+TGG	0.634369	1_3:+33294305	Msa0114920:CDS
CAGGCACGGTGACATGATGA+GGG	0.635211	1_3:-33293826	None:intergenic
AATCTGTAGAAGAATGCCGT+TGG	0.636903	1_3:-33294109	None:intergenic
AGATATTGATCCAAAGCCAA+CGG	0.640968	1_3:+33294093	Msa0114920:CDS
GAAAGATTCTCTGTATTGTG+GGG	0.645886	1_3:+33294172	Msa0114920:CDS
AAATGTCTTTACATCAGGCA+CGG	0.659012	1_3:-33293840	None:intergenic
TATATGATGGCCTTATGTAG+CGG	0.659739	1_3:+33294019	Msa0114920:CDS
ATATGTAGCATGATCTGTAG+TGG	0.696738	1_3:-33293999	None:intergenic
GTATACTGGTGAAGAAAGTG+TGG	0.711055	1_3:+33293871	Msa0114920:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	CATTTTCGTAACTTTTAAAT+AGG	-	chr1_3:33294068-33294087	None:intergenic	20.0%
!!	CTATTTAAAAGTTACGAAAA+TGG	+	chr1_3:33294066-33294085	Msa0114920:CDS	20.0%
!!	AGTAAAACAATTAAGTTGCA+TGG	+	chr1_3:33293800-33293819	Msa0114920:CDS	25.0%
!!	GACGATGTTAAATTGTATTT+TGG	+	chr1_3:33294133-33294152	Msa0114920:CDS	25.0%
!	AGCTTTCATATGAGAATATT+TGG	+	chr1_3:33294281-33294300	Msa0114920:CDS	25.0%
	TCTACAAATGTCTTTACATC+AGG	-	chr1_3:33293848-33293867	None:intergenic	30.0%
	GTGTGGTTGCTATAAAAATA+AGG	+	chr1_3:33293888-33293907	Msa0114920:CDS	30.0%
!	GAGATTTGTTCAAATTCGAA+CGG	+	chr1_3:33293955-33293974	Msa0114920:CDS	30.0%
	AGATCATGCTACATATATGA+TGG	+	chr1_3:33294006-33294025	Msa0114920:CDS	30.0%
!!!	CAATTTTTTTCCGCTACATA+AGG	-	chr1_3:33294032-33294051	None:intergenic	30.0%
!!!	TACTTTGCAGTTTTAAACCA+TGG	-	chr1_3:33294325-33294344	None:intergenic	30.0%
!!	TGCAAAGTATCTATTGATTC+AGG	+	chr1_3:33294336-33294355	Msa0114920:CDS	30.0%
	AAATGTCTTTACATCAGGCA+CGG	-	chr1_3:33293843-33293862	None:intergenic	35.0%
	ACATTTGTAGAGCAGTATAC+TGG	+	chr1_3:33293857-33293876	Msa0114920:CDS	35.0%
	ATATGTAGCATGATCTGTAG+TGG	-	chr1_3:33294002-33294021	None:intergenic	35.0%
!!	TATATGATGGCCTTATGTAG+CGG	+	chr1_3:33294019-33294038	Msa0114920:CDS	35.0%
	AGATATTGATCCAAAGCCAA+CGG	+	chr1_3:33294093-33294112	Msa0114920:CDS	35.0%
	AGGAAAGATTCTCTGTATTG+TGG	+	chr1_3:33294170-33294189	Msa0114920:CDS	35.0%
	GGAAAGATTCTCTGTATTGT+GGG	+	chr1_3:33294171-33294190	Msa0114920:CDS	35.0%
	GAAAGATTCTCTGTATTGTG+GGG	+	chr1_3:33294172-33294191	Msa0114920:CDS	35.0%
	GTTTGAACTTCTTATCGTCA+TGG	-	chr1_3:33294258-33294277	None:intergenic	35.0%
	GTATACTGGTGAAGAAAGTG+TGG	+	chr1_3:33293871-33293890	Msa0114920:CDS	40.0%
	GCGGAAAAAAATTGCAGTTC+TGG	+	chr1_3:33294038-33294057	Msa0114920:CDS	40.0%
	AATCTGTAGAAGAATGCCGT+TGG	-	chr1_3:33294112-33294131	None:intergenic	40.0%
!!	TTTTGGCTGTCAGATCTCAA+AGG	+	chr1_3:33294150-33294169	Msa0114920:CDS	40.0%
!	ATGGATGCTAGTGCAGATTT+TGG	+	chr1_3:33294199-33294218	Msa0114920:CDS	40.0%
!	TGGATGCTAGTGCAGATTTT+GGG	+	chr1_3:33294200-33294219	Msa0114920:CDS	40.0%
	TTGGGATTGGATTGAGAAAG+TGG	+	chr1_3:33294218-33294237	Msa0114920:CDS	40.0%
	AGATTGAACTGCATCAACCA+TGG	+	chr1_3:33294305-33294324	Msa0114920:CDS	40.0%
!!	TAGAAGAATGCCGTTGGCTT+TGG	-	chr1_3:33294106-33294125	None:intergenic	45.0%
	CTCTGTATTGTGGGGAAAGA+TGG	+	chr1_3:33294180-33294199	Msa0114920:CDS	45.0%
!!!	GCTAGTGCAGATTTTGGGAT+TGG	+	chr1_3:33294205-33294224	Msa0114920:CDS	45.0%
	CAGGCACGGTGACATGATGA+GGG	-	chr1_3:33293829-33293848	None:intergenic	55.0%
	TCAGGCACGGTGACATGATG+AGG	-	chr1_3:33293830-33293849	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr1_3	gene	33293791	33294387	33293791	ID=Msa0114920;Name=Msa0114920
chr1_3	mRNA	33293791	33294387	33293791	ID=Msa0114920-mRNA-1;Parent=Msa0114920;Name=Msa0114920-mRNA-1;_AED=0.13;_eAED=0.13;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|198
chr1_3	exon	33293791	33294387	33293791	ID=Msa0114920-mRNA-1:exon:10471;Parent=Msa0114920-mRNA-1
chr1_3	CDS	33293791	33294387	33293791	ID=Msa0114920-mRNA-1:cds;Parent=Msa0114920-mRNA-1

Gene Sequence

>Msa0114920

ATGGTTAAAAGTAAAACAATTAAGTTGCATGGATTCCCTCATCATGTCACCGTGCCTGATGTAAAGACATTTGTAGAGCAGTATACTGGTGAAGAAAGTGTGGTTGCTATAAAAATAAGGAAGTGTAGTTGCTATAAAAATAAGTGTTGTTGCTATAAAAAGAAGAGATTTGTTCAAATTCGAACGGCATTTGCAATTATTCAATTCACCACTACAGATCATGCTACATATATGATGGCCTTATGTAGCGGAAAAAAATTGCAGTTCTGGAAATCCTATTTAAAAGTTACGAAAATGGAAAGAGATATTGATCCAAAGCCAACGGCATTCTTCTACAGATTAGACGATGTTAAATTGTATTTTGGCTGTCAGATCTCAAAGGAAAGATTCTCTGTATTGTGGGGAAAGATGGATGCTAGTGCAGATTTTGGGATTGGATTGAGAAAGTGGCGATTCTCTATGTGCCATGACGATAAGAAGTTCAAACTTGAGCTTTCATATGAGAATATTTGGAAGATTGAACTGCATCAACCATGGTTTAAAACTGCAAAGTATCTATTGATTCAGGTTGTTAAATTCAACTTTTCATTATTTTAA

Protein sequence

>Msa0114920

MVKSKTIKLHGFPHHVTVPDVKTFVEQYTGEESVVAIKIRKCSCYKNKCCCYKKKRFVQIRTAFAIIQFTTTDHATYMMALCSGKKLQFWKSYLKVTKMERDIDPKPTAFFYRLDDVKLYFGCQISKERFSVLWGKMDASADFGIGLRKWRFSMCHDDKKFKLELSYENIWKIELHQPWFKTAKYLLIQVVKFNFSLF*