AlfalfaGEDB | Msa0114940

M. sativa cultivar zhongmu-4 / Msa0114940

KEGG: K11699
GO: GO:0001101 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009605 GO:0009607 GO:0009615 GO:0009719 GO:0009725 GO:0009751 GO:0009892 GO:0009987 GO:0010025 GO:0010033 GO:0010166 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0019222 GO:0030422 GO:0031047 GO:0031050 GO:0034641 GO:0035194 GO:0040029 GO:0042221 GO:0042493 GO:0043170 GO:0043207 GO:0043331 GO:0044237 GO:0044238 GO:0046483 GO:0046677 GO:0048518 GO:0048519 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0060147 GO:0060148 GO:0060255 GO:0060968 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:1901360 GO:1901568 GO:1901570 GO:1901576 GO:1901698 GO:1901699 GO:1901700
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0114940	KEH40644.1	67.539	191	47	2	1	191	1	176	3.22e-74	249

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0114940	sp\|Q9LQV2\|RDR1_ARATH	40.426	188	97	3	4	191	2	174	1.50e-40	149

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0114940	A0A072VF31	67.539	191	47	2	1	191	1	176	1.54e-74	249

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0114910	Msa0114940	0.948762	4.951675e-107	-8.615850e-47
Msa0114920	Msa0114940	0.945381	3.399259e-104	-8.615850e-47
Msa0114930	Msa0114940	0.949928	4.701505e-108	-8.615850e-47
Msa0114940	Msa0114950	0.925169	2.640821e-90	-8.615850e-47
Msa0114940	Msa0114970	0.943851	5.696291e-103	-8.615850e-47
Msa0114940	Msa0114980	0.903007	5.411632e-79	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0114940	MtrunA17_Chr1g0161491	67.539	191	47	2	1	191	15	190	1.43e-76	248
Msa0114940	MtrunA17_Chr1g0161471	65.285	193	51	2	1	191	1	179	2.62e-76	248
Msa0114940	MtrunA17_Chr1g0161511	62.887	194	54	4	1	191	1	179	6.84e-69	227
Msa0114940	MtrunA17_Chr1g0161521	62.887	194	54	4	1	191	1	179	1.20e-68	226
Msa0114940	MtrunA17_Chr1g0161451	33.871	186	108	3	5	190	3	173	3.86e-27	107

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0114940	AT1G14790.1	40.426	188	97	3	4	191	2	174	1.52e-41	149

Find 26 sgRNAs with CRISPR-Local

Find 34 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GACGATGTTAAATTGTATTT+TGG	0.150856	1_3:+33349197	Msa0114940:CDS
AGCTTTCATATGAGAATATT+TGG	0.191630	1_3:+33349345	Msa0114940:CDS
ATGGATGCTAGTGCAGATTT+TGG	0.192772	1_3:+33349263	Msa0114940:CDS
CTATTTAAAAGTTACGAAAA+TGG	0.261211	1_3:+33349130	Msa0114940:CDS
TGCAAAGTATCTATTGATTC+AGG	0.268018	1_3:+33349400	Msa0114940:CDS
TAGAAGAATGCCGTTGGCTT+TGG	0.337561	1_3:-33349167	None:intergenic
CTCTGTATTGTGGGGAAAGA+TGG	0.454381	1_3:+33349244	Msa0114940:CDS
TTGGGATTGGATTGAGAAAG+TGG	0.455481	1_3:+33349282	Msa0114940:CDS
ACATTTGTAGAGCAGTATAC+TGG	0.461229	1_3:+33348921	Msa0114940:CDS
GGAAAGATTCTCTGTATTGT+GGG	0.467936	1_3:+33349235	Msa0114940:CDS
TCTACAAATGTCTTTACATC+AGG	0.480962	1_3:-33348909	None:intergenic
AGTAAAACAATTAAGTTGCA+TGG	0.485965	1_3:+33348864	Msa0114940:CDS
AGGAAAGATTCTCTGTATTG+TGG	0.507749	1_3:+33349234	Msa0114940:CDS
AGATCATGCTACATATATGA+TGG	0.530126	1_3:+33349070	Msa0114940:CDS
GTTTGAACTTCTTATCGTCA+TGG	0.543349	1_3:-33349319	None:intergenic
GAGATTTGTTCAAATTCGAA+CGG	0.545671	1_3:+33349019	Msa0114940:CDS
TCAGGCACGGTGACATGATG+AGG	0.567959	1_3:-33348891	None:intergenic
AGATTGAACTGCATCAACCA+TGG	0.634369	1_3:+33349369	Msa0114940:CDS
CAGGCACGGTGACATGATGA+GGG	0.635211	1_3:-33348890	None:intergenic
AATCTGTAGAAGAATGCCGT+TGG	0.636903	1_3:-33349173	None:intergenic
AGATATTGATCCAAAGCCAA+CGG	0.640968	1_3:+33349157	Msa0114940:CDS
GAAAGATTCTCTGTATTGTG+GGG	0.645886	1_3:+33349236	Msa0114940:CDS
AAATGTCTTTACATCAGGCA+CGG	0.659012	1_3:-33348904	None:intergenic
TATATGATGGCCTTATGTAG+CGG	0.659739	1_3:+33349083	Msa0114940:CDS
ATATGTAGCATGATCTGTAG+TGG	0.696738	1_3:-33349063	None:intergenic
GTATACTGGTGAAGAAAGTG+TGG	0.711055	1_3:+33348935	Msa0114940:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	CATTTTCGTAACTTTTAAAT+AGG	-	chr1_3:33349132-33349151	None:intergenic	20.0%
!!	CTATTTAAAAGTTACGAAAA+TGG	+	chr1_3:33349130-33349149	Msa0114940:CDS	20.0%
!!	AGTAAAACAATTAAGTTGCA+TGG	+	chr1_3:33348864-33348883	Msa0114940:CDS	25.0%
!!	GACGATGTTAAATTGTATTT+TGG	+	chr1_3:33349197-33349216	Msa0114940:CDS	25.0%
!	AGCTTTCATATGAGAATATT+TGG	+	chr1_3:33349345-33349364	Msa0114940:CDS	25.0%
	TCTACAAATGTCTTTACATC+AGG	-	chr1_3:33348912-33348931	None:intergenic	30.0%
	GTGTGGTTGCTATAAAAATA+AGG	+	chr1_3:33348952-33348971	Msa0114940:CDS	30.0%
!	GAGATTTGTTCAAATTCGAA+CGG	+	chr1_3:33349019-33349038	Msa0114940:CDS	30.0%
	AGATCATGCTACATATATGA+TGG	+	chr1_3:33349070-33349089	Msa0114940:CDS	30.0%
!!!	CAATTTTTTTCCGCTACATA+AGG	-	chr1_3:33349096-33349115	None:intergenic	30.0%
!!!	TACTTTGCAGTTTTAAACCA+TGG	-	chr1_3:33349389-33349408	None:intergenic	30.0%
!!	TGCAAAGTATCTATTGATTC+AGG	+	chr1_3:33349400-33349419	Msa0114940:CDS	30.0%
	AAATGTCTTTACATCAGGCA+CGG	-	chr1_3:33348907-33348926	None:intergenic	35.0%
	ACATTTGTAGAGCAGTATAC+TGG	+	chr1_3:33348921-33348940	Msa0114940:CDS	35.0%
	ATATGTAGCATGATCTGTAG+TGG	-	chr1_3:33349066-33349085	None:intergenic	35.0%
!!	TATATGATGGCCTTATGTAG+CGG	+	chr1_3:33349083-33349102	Msa0114940:CDS	35.0%
	AGATATTGATCCAAAGCCAA+CGG	+	chr1_3:33349157-33349176	Msa0114940:CDS	35.0%
	AGGAAAGATTCTCTGTATTG+TGG	+	chr1_3:33349234-33349253	Msa0114940:CDS	35.0%
	GGAAAGATTCTCTGTATTGT+GGG	+	chr1_3:33349235-33349254	Msa0114940:CDS	35.0%
	GAAAGATTCTCTGTATTGTG+GGG	+	chr1_3:33349236-33349255	Msa0114940:CDS	35.0%
	GTTTGAACTTCTTATCGTCA+TGG	-	chr1_3:33349322-33349341	None:intergenic	35.0%
	GTATACTGGTGAAGAAAGTG+TGG	+	chr1_3:33348935-33348954	Msa0114940:CDS	40.0%
	GCGGAAAAAAATTGCAGTTC+TGG	+	chr1_3:33349102-33349121	Msa0114940:CDS	40.0%
	AATCTGTAGAAGAATGCCGT+TGG	-	chr1_3:33349176-33349195	None:intergenic	40.0%
!!	TTTTGGCTGTCAGATCTCAA+AGG	+	chr1_3:33349214-33349233	Msa0114940:CDS	40.0%
!	ATGGATGCTAGTGCAGATTT+TGG	+	chr1_3:33349263-33349282	Msa0114940:CDS	40.0%
!	TGGATGCTAGTGCAGATTTT+GGG	+	chr1_3:33349264-33349283	Msa0114940:CDS	40.0%
	TTGGGATTGGATTGAGAAAG+TGG	+	chr1_3:33349282-33349301	Msa0114940:CDS	40.0%
	AGATTGAACTGCATCAACCA+TGG	+	chr1_3:33349369-33349388	Msa0114940:CDS	40.0%
!!	TAGAAGAATGCCGTTGGCTT+TGG	-	chr1_3:33349170-33349189	None:intergenic	45.0%
	CTCTGTATTGTGGGGAAAGA+TGG	+	chr1_3:33349244-33349263	Msa0114940:CDS	45.0%
!!!	GCTAGTGCAGATTTTGGGAT+TGG	+	chr1_3:33349269-33349288	Msa0114940:CDS	45.0%
	CAGGCACGGTGACATGATGA+GGG	-	chr1_3:33348893-33348912	None:intergenic	55.0%
	TCAGGCACGGTGACATGATG+AGG	-	chr1_3:33348894-33348913	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr1_3	gene	33348855	33349451	33348855	ID=Msa0114940;Name=Msa0114940
chr1_3	mRNA	33348855	33349451	33348855	ID=Msa0114940-mRNA-1;Parent=Msa0114940;Name=Msa0114940-mRNA-1;_AED=0.13;_eAED=0.13;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|198
chr1_3	exon	33348855	33349451	33348855	ID=Msa0114940-mRNA-1:exon:10473;Parent=Msa0114940-mRNA-1
chr1_3	CDS	33348855	33349451	33348855	ID=Msa0114940-mRNA-1:cds;Parent=Msa0114940-mRNA-1

Gene Sequence

>Msa0114940

ATGGTTAAAAGTAAAACAATTAAGTTGCATGGATTCCCTCATCATGTCACCGTGCCTGATGTAAAGACATTTGTAGAGCAGTATACTGGTGAAGAAAGTGTGGTTGCTATAAAAATAAGGAAGTGTAGTTGCTATAAAAATAAGTGTTGTTGCTATAAAAAGAAGAGATTTGTTCAAATTCGAACGGCATTTGCAATTATTCAATTCACCACTACAGATCATGCTACATATATGATGGCCTTATGTAGCGGAAAAAAATTGCAGTTCTGGAAATCCTATTTAAAAGTTACGAAAATGGAAAGAGATATTGATCCAAAGCCAACGGCATTCTTCTACAGATTAGACGATGTTAAATTGTATTTTGGCTGTCAGATCTCAAAGGAAAGATTCTCTGTATTGTGGGGAAAGATGGATGCTAGTGCAGATTTTGGGATTGGATTGAGAAAGTGGCGATTCTCTATGTGCCATGACGATAAGAAGTTCAAACTTGAGCTTTCATATGAGAATATTTGGAAGATTGAACTGCATCAACCATGGTTTAAAACTGCAAAGTATCTATTGATTCAGGTTGTTAAATTCAACTTTTCATTATTTTAA

Protein sequence

>Msa0114940

MVKSKTIKLHGFPHHVTVPDVKTFVEQYTGEESVVAIKIRKCSCYKNKCCCYKKKRFVQIRTAFAIIQFTTTDHATYMMALCSGKKLQFWKSYLKVTKMERDIDPKPTAFFYRLDDVKLYFGCQISKERFSVLWGKMDASADFGIGLRKWRFSMCHDDKKFKLELSYENIWKIELHQPWFKTAKYLLIQVVKFNFSLF*