Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0118670 | Msa0886380 | 0.825903 | 3.635803e-54 | -8.615850e-47 |
| Msa0118670 | Msa1398630 | 0.885933 | 5.301028e-72 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 22 sgRNAs with CRISPR-Local
Find 25 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGATAACAAAATACGTTTA+TGG | 0.231001 | 1_3:+41765434 | Msa0118670:CDS |
| TGAGTTAAATCATTTATTCT+TGG | 0.317394 | 1_3:-41765816 | None:intergenic |
| TCAAATAATAATTTATTAGT+AGG | 0.327209 | 1_3:-41765332 | None:intergenic |
| GTAGCGGAAGCTTATCTTGC+CGG | 0.337900 | 1_3:+41765558 | Msa0118670:intron |
| AGAAGATATAAACACAATTA+TGG | 0.357622 | 1_3:+41765622 | Msa0118670:CDS |
| AGGCTGACCATCCTCATTAA+AGG | 0.357925 | 1_3:-41765303 | None:intergenic |
| GTACTCTACTTTACATCAAT+TGG | 0.361418 | 1_3:+41765398 | Msa0118670:CDS |
| AATTTATTAGTAGGCGCAAT+AGG | 0.362229 | 1_3:-41765323 | None:intergenic |
| GCCGGTTGAAGCACGAGATT+GGG | 0.393888 | 1_3:+41765576 | Msa0118670:CDS |
| TGCCGGTTGAAGCACGAGAT+TGG | 0.404804 | 1_3:+41765575 | Msa0118670:CDS |
| AAAGATTCATGGAAGAACTT+TGG | 0.409409 | 1_3:+41765261 | None:intergenic |
| ATTTGAGTTGAGACTCGTCT+TGG | 0.461173 | 1_3:+41765349 | Msa0118670:CDS |
| ACCCAATCTCGTGCTTCAAC+CGG | 0.485424 | 1_3:-41765577 | None:intergenic |
| CTACTTTACATCAATTGGTA+AGG | 0.503866 | 1_3:+41765403 | Msa0118670:CDS |
| TTCAAGACAGATGAATGAGT+TGG | 0.504006 | 1_3:+41765650 | Msa0118670:exon |
| TTTGAGTTGAGACTCGTCTT+GGG | 0.506918 | 1_3:+41765350 | Msa0118670:CDS |
| TCATGGAAGAACTTTGGAAG+AGG | 0.542750 | 1_3:+41765267 | None:intergenic |
| GAGACTCGTCTTGGGATCAA+TGG | 0.548154 | 1_3:+41765358 | Msa0118670:CDS |
| GAAGTGACCTTTAATGAGGA+TGG | 0.557015 | 1_3:+41765296 | Msa0118670:CDS |
| TTGAGAATACTTAACATGCA+TGG | 0.569474 | 1_3:+41765599 | Msa0118670:CDS |
| AGAAGAAGTGACCTTTAATG+AGG | 0.599762 | 1_3:+41765292 | Msa0118670:CDS |
| TCGTCTTGGGATCAATGGCT+AGG | 0.605776 | 1_3:+41765363 | Msa0118670:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TCAAATAATAATTTATTAGT+AGG | - | chr1_3:41765335-41765354 | None:intergenic | 10.0% |
| !!! | AAACGTATTTTGTTATCTTT+AGG | - | chr1_3:41765433-41765452 | None:intergenic | 20.0% |
| !! | AAGATAACAAAATACGTTTA+TGG | + | chr1_3:41765434-41765453 | Msa0118670:CDS | 20.0% |
| !! | AGAAGATATAAACACAATTA+TGG | + | chr1_3:41765622-41765641 | Msa0118670:CDS | 20.0% |
| !!! | AGTTGGAAGATTTTATATTT+AGG | + | chr1_3:41765667-41765686 | Msa0118670:exon | 20.0% |
| !! | TGAGTTAAATCATTTATTCT+TGG | - | chr1_3:41765819-41765838 | None:intergenic | 20.0% |
| !!! | ATGATTTAACTCAATTTTTG+CGG | + | chr1_3:41765826-41765845 | Msa0118670:three_prime_UTR | 20.0% |
| !! | TTGTCTTATAACTTTTGTAG+CGG | + | chr1_3:41765542-41765561 | Msa0118670:intron | 25.0% |
| !! | ACAGTACTATGTATTTTGTT+AGG | + | chr1_3:41765715-41765734 | Msa0118670:three_prime_UTR | 25.0% |
| AATTTATTAGTAGGCGCAAT+AGG | - | chr1_3:41765326-41765345 | None:intergenic | 30.0% | |
| GTACTCTACTTTACATCAAT+TGG | + | chr1_3:41765398-41765417 | Msa0118670:CDS | 30.0% | |
| CTACTTTACATCAATTGGTA+AGG | + | chr1_3:41765403-41765422 | Msa0118670:CDS | 30.0% | |
| TTGAGAATACTTAACATGCA+TGG | + | chr1_3:41765599-41765618 | Msa0118670:CDS | 30.0% | |
| AGAAGAAGTGACCTTTAATG+AGG | + | chr1_3:41765292-41765311 | Msa0118670:CDS | 35.0% | |
| TTCAAGACAGATGAATGAGT+TGG | + | chr1_3:41765650-41765669 | Msa0118670:exon | 35.0% | |
| GAAGTGACCTTTAATGAGGA+TGG | + | chr1_3:41765296-41765315 | Msa0118670:CDS | 40.0% | |
| !! | ATTTGAGTTGAGACTCGTCT+TGG | + | chr1_3:41765349-41765368 | Msa0118670:CDS | 40.0% |
| !! | TTTGAGTTGAGACTCGTCTT+GGG | + | chr1_3:41765350-41765369 | Msa0118670:CDS | 40.0% |
| AGGCTGACCATCCTCATTAA+AGG | - | chr1_3:41765306-41765325 | None:intergenic | 45.0% | |
| GAGACTCGTCTTGGGATCAA+TGG | + | chr1_3:41765358-41765377 | Msa0118670:CDS | 50.0% | |
| TCGTCTTGGGATCAATGGCT+AGG | + | chr1_3:41765363-41765382 | Msa0118670:CDS | 50.0% | |
| ! | GTAGCGGAAGCTTATCTTGC+CGG | + | chr1_3:41765558-41765577 | Msa0118670:intron | 50.0% |
| ACCCAATCTCGTGCTTCAAC+CGG | - | chr1_3:41765580-41765599 | None:intergenic | 50.0% | |
| ! | TGCCGGTTGAAGCACGAGAT+TGG | + | chr1_3:41765575-41765594 | Msa0118670:CDS | 55.0% |
| GCCGGTTGAAGCACGAGATT+GGG | + | chr1_3:41765576-41765595 | Msa0118670:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_3 | gene | 41765269 | 41765855 | 41765269 | ID=Msa0118670;Name=Msa0118670 |
| chr1_3 | mRNA | 41765269 | 41765855 | 41765269 | ID=Msa0118670-mRNA-1;Parent=Msa0118670;Name=Msa0118670-mRNA-1;_AED=0.32;_eAED=0.55;_QI=0|0|0.5|1|1|1|2|188|101 |
| chr1_3 | exon | 41765269 | 41765468 | 41765269 | ID=Msa0118670-mRNA-1:exon:12649;Parent=Msa0118670-mRNA-1 |
| chr1_3 | exon | 41765562 | 41765855 | 41765562 | ID=Msa0118670-mRNA-1:exon:12650;Parent=Msa0118670-mRNA-1 |
| chr1_3 | CDS | 41765269 | 41765468 | 41765269 | ID=Msa0118670-mRNA-1:cds;Parent=Msa0118670-mRNA-1 |
| chr1_3 | CDS | 41765562 | 41765667 | 41765562 | ID=Msa0118670-mRNA-1:cds;Parent=Msa0118670-mRNA-1 |
| chr1_3 | three_prime_UTR | 41765668 | 41765855 | 41765668 | ID=Msa0118670-mRNA-1:three_prime_utr;Parent=Msa0118670-mRNA-1 |
| Gene Sequence |
| Protein sequence |
