Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0118670 | Msa1398630 | 0.885933 | 5.301028e-72 | -8.615850e-47 |
| Msa1369530 | Msa1398630 | 0.811386 | 7.184065e-51 | -8.615850e-47 |
| Msa1398630 | Msa1461250 | -0.800543 | 1.372229e-48 | -8.615850e-47 |
| Msa0800760 | Msa1398630 | 0.805464 | 1.318538e-49 | -8.615850e-47 |
| Msa0838940 | Msa1398630 | 0.814803 | 1.279364e-51 | -8.615850e-47 |
| Msa0160620 | Msa1398630 | 0.805102 | 1.569973e-49 | -8.615850e-47 |
| Msa0442850 | Msa1398630 | 0.808884 | 2.487281e-50 | -8.615850e-47 |
| Msa0449760 | Msa1398630 | 0.810717 | 1.003188e-50 | -8.615850e-47 |
| Msa0344830 | Msa1398630 | 0.805374 | 1.376452e-49 | -8.615850e-47 |
| Msa1205130 | Msa1398630 | 0.816771 | 4.656735e-52 | -8.615850e-47 |
| Msa0886380 | Msa1398630 | 0.801786 | 7.639033e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 22 sgRNAs with CRISPR-Local
Find 25 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGATAACAAAATACGTTTA+TGG | 0.231001 | tig0022273:+28865 | Msa1398630:CDS |
| TGAGTTAAATCATTTATTCT+TGG | 0.317394 | tig0022273:-29244 | None:intergenic |
| TCAAATAATAATTTATTAGT+AGG | 0.327209 | tig0022273:-28763 | None:intergenic |
| GTAGCGGAAGCTTATCTTGC+CGG | 0.337900 | tig0022273:+28987 | Msa1398630:intron |
| AGAAGATATAAACACAATTA+TGG | 0.357622 | tig0022273:+29051 | Msa1398630:CDS |
| AGGCTGACCATCCTCATTAA+AGG | 0.357925 | tig0022273:-28734 | None:intergenic |
| GTACTCTACTTTACATCAAT+TGG | 0.361418 | tig0022273:+28829 | Msa1398630:CDS |
| AATTTATTAGTAGGCGCAAT+AGG | 0.362229 | tig0022273:-28754 | None:intergenic |
| GCCGGTTGAAGCACGAGATT+GGG | 0.393888 | tig0022273:+29005 | Msa1398630:CDS |
| TGCCGGTTGAAGCACGAGAT+TGG | 0.404804 | tig0022273:+29004 | Msa1398630:CDS |
| AAAGATTCATGGAAGAACTT+TGG | 0.409409 | tig0022273:+28692 | None:intergenic |
| ATTTGAGTTGAGACTCGTCT+TGG | 0.461173 | tig0022273:+28780 | Msa1398630:CDS |
| ACCCAATCTCGTGCTTCAAC+CGG | 0.485424 | tig0022273:-29006 | None:intergenic |
| CTACTTTACATCAATTGGTA+AGG | 0.503866 | tig0022273:+28834 | Msa1398630:CDS |
| TTTGAGTTGAGACTCGTCTT+GGG | 0.506918 | tig0022273:+28781 | Msa1398630:CDS |
| TTCAACACAGATGAATGAGT+TGG | 0.522447 | tig0022273:+29079 | Msa1398630:exon |
| TCATGGAAGAACTTTGGAAG+AGG | 0.542750 | tig0022273:+28698 | None:intergenic |
| GAGACTCGTCTTGGGATCAA+TGG | 0.548154 | tig0022273:+28789 | Msa1398630:CDS |
| GAAGTGACCTTTAATGAGGA+TGG | 0.557015 | tig0022273:+28727 | Msa1398630:CDS |
| TTGAGAATACTTAACATGCA+TGG | 0.569474 | tig0022273:+29028 | Msa1398630:CDS |
| AGAAGAAGTGACCTTTAATG+AGG | 0.599762 | tig0022273:+28723 | Msa1398630:CDS |
| TCGTCTTGGGATCAATGGCT+AGG | 0.605776 | tig0022273:+28794 | Msa1398630:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TCAAATAATAATTTATTAGT+AGG | - | tig0022273:28766-28785 | None:intergenic | 10.0% |
| !!! | AAACGTATTTTGTTATCTTT+AGG | - | tig0022273:28864-28883 | None:intergenic | 20.0% |
| !! | AAGATAACAAAATACGTTTA+TGG | + | tig0022273:28865-28884 | Msa1398630:CDS | 20.0% |
| !! | AGAAGATATAAACACAATTA+TGG | + | tig0022273:29051-29070 | Msa1398630:CDS | 20.0% |
| !!! | AGTTGGAAGATTTTATATTT+AGG | + | tig0022273:29096-29115 | Msa1398630:exon | 20.0% |
| !! | TGAGTTAAATCATTTATTCT+TGG | - | tig0022273:29247-29266 | None:intergenic | 20.0% |
| !!! | ATGATTTAACTCAATTTTTG+CGG | + | tig0022273:29254-29273 | Msa1398630:three_prime_UTR | 20.0% |
| !! | TTGTCTTATAACTTTTGTAG+CGG | + | tig0022273:28971-28990 | Msa1398630:intron | 25.0% |
| !! | ACAGTACTATGTATTTTGTT+AGG | + | tig0022273:29144-29163 | Msa1398630:three_prime_UTR | 25.0% |
| AATTTATTAGTAGGCGCAAT+AGG | - | tig0022273:28757-28776 | None:intergenic | 30.0% | |
| GTACTCTACTTTACATCAAT+TGG | + | tig0022273:28829-28848 | Msa1398630:CDS | 30.0% | |
| CTACTTTACATCAATTGGTA+AGG | + | tig0022273:28834-28853 | Msa1398630:CDS | 30.0% | |
| TTGAGAATACTTAACATGCA+TGG | + | tig0022273:29028-29047 | Msa1398630:CDS | 30.0% | |
| AGAAGAAGTGACCTTTAATG+AGG | + | tig0022273:28723-28742 | Msa1398630:CDS | 35.0% | |
| TTCAACACAGATGAATGAGT+TGG | + | tig0022273:29079-29098 | Msa1398630:exon | 35.0% | |
| GAAGTGACCTTTAATGAGGA+TGG | + | tig0022273:28727-28746 | Msa1398630:CDS | 40.0% | |
| !! | ATTTGAGTTGAGACTCGTCT+TGG | + | tig0022273:28780-28799 | Msa1398630:CDS | 40.0% |
| !! | TTTGAGTTGAGACTCGTCTT+GGG | + | tig0022273:28781-28800 | Msa1398630:CDS | 40.0% |
| AGGCTGACCATCCTCATTAA+AGG | - | tig0022273:28737-28756 | None:intergenic | 45.0% | |
| GAGACTCGTCTTGGGATCAA+TGG | + | tig0022273:28789-28808 | Msa1398630:CDS | 50.0% | |
| TCGTCTTGGGATCAATGGCT+AGG | + | tig0022273:28794-28813 | Msa1398630:CDS | 50.0% | |
| ! | GTAGCGGAAGCTTATCTTGC+CGG | + | tig0022273:28987-29006 | Msa1398630:intron | 50.0% |
| ACCCAATCTCGTGCTTCAAC+CGG | - | tig0022273:29009-29028 | None:intergenic | 50.0% | |
| ! | TGCCGGTTGAAGCACGAGAT+TGG | + | tig0022273:29004-29023 | Msa1398630:CDS | 55.0% |
| GCCGGTTGAAGCACGAGATT+GGG | + | tig0022273:29005-29024 | Msa1398630:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0022273 | gene | 28700 | 29283 | 28700 | ID=Msa1398630;Name=Msa1398630 |
| tig0022273 | mRNA | 28700 | 29283 | 28700 | ID=Msa1398630-mRNA-1;Parent=Msa1398630;Name=Msa1398630-mRNA-1;_AED=0.37;_eAED=0.58;_QI=0|0|0.5|1|1|1|2|187|101 |
| tig0022273 | exon | 28700 | 28899 | 28700 | ID=Msa1398630-mRNA-1:exon:16750;Parent=Msa1398630-mRNA-1 |
| tig0022273 | exon | 28991 | 29283 | 28991 | ID=Msa1398630-mRNA-1:exon:16751;Parent=Msa1398630-mRNA-1 |
| tig0022273 | CDS | 28700 | 28899 | 28700 | ID=Msa1398630-mRNA-1:cds;Parent=Msa1398630-mRNA-1 |
| tig0022273 | CDS | 28991 | 29096 | 28991 | ID=Msa1398630-mRNA-1:cds;Parent=Msa1398630-mRNA-1 |
| tig0022273 | three_prime_UTR | 29097 | 29283 | 29097 | ID=Msa1398630-mRNA-1:three_prime_utr;Parent=Msa1398630-mRNA-1 |
| Gene Sequence |
| Protein sequence |
