Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0118670 | Msa0886380 | 0.825903 | 3.635803e-54 | -8.615850e-47 |
| Msa0886380 | Msa1398630 | 0.801786 | 7.639033e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 24 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGATAACAAAATACGTTTA+TGG | 0.231001 | 6_1:-44958332 | Msa0886380:CDS |
| TGAGTTAAATCATTTATTCT+TGG | 0.317394 | 6_1:+44957953 | None:intergenic |
| TCAAATAATAATTTATTAGT+AGG | 0.327209 | 6_1:+44958434 | None:intergenic |
| GTAGCGGAAGCTTATCTTGC+CGG | 0.337900 | 6_1:-44958210 | Msa0886380:intron |
| GCCGGTTGAAGCACGGGATT+GGG | 0.352168 | 6_1:-44958192 | Msa0886380:CDS |
| AGAAGATATAAACACAATTA+TGG | 0.357622 | 6_1:-44958146 | Msa0886380:CDS |
| AGGCTGACCATCCTCATTAA+AGG | 0.357925 | 6_1:+44958463 | None:intergenic |
| GTACTCTACTTTACATCAAT+TGG | 0.361418 | 6_1:-44958368 | Msa0886380:CDS |
| AATTTATTAGTAGGCGCAAT+AGG | 0.362229 | 6_1:+44958443 | None:intergenic |
| TGCCGGTTGAAGCACGGGAT+TGG | 0.374842 | 6_1:-44958193 | Msa0886380:CDS |
| ATTTGAGTTGAGACTCGTAT+TGG | 0.392066 | 6_1:-44958417 | Msa0886380:CDS |
| AAAGATTCATGGAAGAACTT+TGG | 0.409409 | 6_1:-44958505 | None:intergenic |
| ACCCAATCCCGTGCTTCAAC+CGG | 0.412209 | 6_1:+44958191 | None:intergenic |
| TTTGAGTTGAGACTCGTATT+GGG | 0.424478 | 6_1:-44958416 | Msa0886380:CDS |
| TATCTTGCCGGTTGAAGCAC+GGG | 0.473256 | 6_1:-44958198 | Msa0886380:CDS |
| CTACTTTACATCAATTGGTA+AGG | 0.503866 | 6_1:-44958363 | Msa0886380:CDS |
| TTCAAGACAGATGAATGAGT+TGG | 0.504006 | 6_1:-44958118 | Msa0886380:exon |
| TCATGGAAGAACTTTGGAAG+AGG | 0.542750 | 6_1:-44958499 | Msa0886380:CDS |
| GAGACTCGTATTGGGATCAA+TGG | 0.546777 | 6_1:-44958408 | Msa0886380:CDS |
| TTATCTTGCCGGTTGAAGCA+CGG | 0.556748 | 6_1:-44958199 | Msa0886380:CDS |
| GAAGTGACCTTTAATGAGGA+TGG | 0.557015 | 6_1:-44958470 | Msa0886380:CDS |
| TTGAGAATACTTAACATGCA+TGG | 0.569474 | 6_1:-44958169 | Msa0886380:CDS |
| AGAAGAAGTGACCTTTAATG+AGG | 0.599762 | 6_1:-44958474 | Msa0886380:CDS |
| TCGTATTGGGATCAATGGCA+AGG | 0.652045 | 6_1:-44958403 | Msa0886380:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TCAAATAATAATTTATTAGT+AGG | + | chr6_1:44958002-44958021 | None:intergenic | 10.0% |
| !!! | AAACGTATTTTGTTATCTTT+AGG | + | chr6_1:44958100-44958119 | None:intergenic | 20.0% |
| !! | AAGATAACAAAATACGTTTA+TGG | - | chr6_1:44958101-44958120 | Msa0886380:three_prime_UTR | 20.0% |
| !!! | TTATCTTATAACTTTTGTAG+CGG | - | chr6_1:44958207-44958226 | Msa0886380:CDS | 20.0% |
| !! | AGAAGATATAAACACAATTA+TGG | - | chr6_1:44958287-44958306 | Msa0886380:intron | 20.0% |
| !!! | AGTTGGAAGATTTTATATTT+AGG | - | chr6_1:44958332-44958351 | Msa0886380:CDS | 20.0% |
| !!! | TGTATTTTCTTAAGTAATGT+TGG | - | chr6_1:44958410-44958429 | Msa0886380:CDS | 20.0% |
| !! | TGAGTTAAATCATTTATTCT+TGG | + | chr6_1:44958483-44958502 | None:intergenic | 20.0% |
| !!! | ATGATTTAACTCAATTTTTG+CGG | - | chr6_1:44958490-44958509 | Msa0886380:CDS | 20.0% |
| !! | ACAGTACTATGTATTTTGTT+AGG | - | chr6_1:44958380-44958399 | Msa0886380:CDS | 25.0% |
| AATTTATTAGTAGGCGCAAT+AGG | + | chr6_1:44957993-44958012 | None:intergenic | 30.0% | |
| GTACTCTACTTTACATCAAT+TGG | - | chr6_1:44958065-44958084 | Msa0886380:three_prime_UTR | 30.0% | |
| CTACTTTACATCAATTGGTA+AGG | - | chr6_1:44958070-44958089 | Msa0886380:three_prime_UTR | 30.0% | |
| TTGAGAATACTTAACATGCA+TGG | - | chr6_1:44958264-44958283 | Msa0886380:intron | 30.0% | |
| AGAAGAAGTGACCTTTAATG+AGG | - | chr6_1:44957959-44957978 | Msa0886380:three_prime_UTR | 35.0% | |
| !! | ATTTGAGTTGAGACTCGTAT+TGG | - | chr6_1:44958016-44958035 | Msa0886380:three_prime_UTR | 35.0% |
| !! | TTTGAGTTGAGACTCGTATT+GGG | - | chr6_1:44958017-44958036 | Msa0886380:three_prime_UTR | 35.0% |
| TTCAAGACAGATGAATGAGT+TGG | - | chr6_1:44958315-44958334 | Msa0886380:intron | 35.0% | |
| GAAGTGACCTTTAATGAGGA+TGG | - | chr6_1:44957963-44957982 | Msa0886380:three_prime_UTR | 40.0% | |
| AGGCTGACCATCCTCATTAA+AGG | + | chr6_1:44957973-44957992 | None:intergenic | 45.0% | |
| GAGACTCGTATTGGGATCAA+TGG | - | chr6_1:44958025-44958044 | Msa0886380:three_prime_UTR | 45.0% | |
| TCGTATTGGGATCAATGGCA+AGG | - | chr6_1:44958030-44958049 | Msa0886380:three_prime_UTR | 45.0% | |
| ! | TTATCTTGCCGGTTGAAGCA+CGG | - | chr6_1:44958234-44958253 | Msa0886380:intron | 45.0% |
| ! | GTAGCGGAAGCTTATCTTGC+CGG | - | chr6_1:44958223-44958242 | Msa0886380:intron | 50.0% |
| ! | TATCTTGCCGGTTGAAGCAC+GGG | - | chr6_1:44958235-44958254 | Msa0886380:intron | 50.0% |
| ACCCAATCCCGTGCTTCAAC+CGG | + | chr6_1:44958245-44958264 | None:intergenic | 55.0% | |
| ! | TGCCGGTTGAAGCACGGGAT+TGG | - | chr6_1:44958240-44958259 | Msa0886380:intron | 60.0% |
| GCCGGTTGAAGCACGGGATT+GGG | - | chr6_1:44958241-44958260 | Msa0886380:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_1 | gene | 44957936 | 44958519 | 44957936 | ID=Msa0886380;Name=Msa0886380 |
| chr6_1 | mRNA | 44957936 | 44958519 | 44957936 | ID=Msa0886380-mRNA-1;Parent=Msa0886380;Name=Msa0886380-mRNA-1;_AED=0.37;_eAED=0.58;_QI=0|0|0.5|1|1|1|2|187|101 |
| chr6_1 | exon | 44957936 | 44958228 | 44957936 | ID=Msa0886380-mRNA-1:exon:9629;Parent=Msa0886380-mRNA-1 |
| chr6_1 | exon | 44958320 | 44958519 | 44958320 | ID=Msa0886380-mRNA-1:exon:9628;Parent=Msa0886380-mRNA-1 |
| chr6_1 | CDS | 44958320 | 44958519 | 44958320 | ID=Msa0886380-mRNA-1:cds;Parent=Msa0886380-mRNA-1 |
| chr6_1 | CDS | 44958123 | 44958228 | 44958123 | ID=Msa0886380-mRNA-1:cds;Parent=Msa0886380-mRNA-1 |
| chr6_1 | three_prime_UTR | 44957936 | 44958122 | 44957936 | ID=Msa0886380-mRNA-1:three_prime_utr;Parent=Msa0886380-mRNA-1 |
| Gene Sequence |
| Protein sequence |
