AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0121960

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0121960 MtrunA17_Chr1g0181481 89.073 302 32 1 23 323 23 324 0.0 561
Msa0121960 MtrunA17_Chr1g0181431 84.106 302 47 1 23 323 23 324 0.0 533
Msa0121960 MtrunA17_Chr1g0181511 81.481 324 59 1 1 323 1 324 0.0 523
Msa0121960 MtrunA17_Chr1g0181501 96.825 189 5 1 135 323 3 190 4.60e-134 379
Msa0121960 MtrunA17_Chr4g0074851 55.352 327 136 3 8 324 4 330 1.15e-129 373
Msa0121960 MtrunA17_Chr1g0181491 98.214 112 2 0 24 135 9 120 2.32e-77 233
Msa0121960 MtrunA17_Chr4g0075241 36.991 319 194 5 9 322 11 327 7.25e-71 223
Msa0121960 MtrunA17_Chr5g0404721 40.000 300 169 5 31 322 34 330 9.14e-68 215
Msa0121960 MtrunA17_Chr7g0254201 36.808 307 183 5 22 324 15 314 6.70e-65 207
Msa0121960 MtrunA17_Chr3g0106921 35.220 318 200 5 8 322 6 320 2.15e-64 206
Msa0121960 MtrunA17_Chr8g0372601 38.291 316 179 7 17 322 21 330 9.58e-64 204
Msa0121960 MtrunA17_Chr7g0254231 37.667 300 179 4 25 323 20 312 1.49e-62 201
Msa0121960 MtrunA17_Chr3g0129051 35.110 319 187 7 9 319 13 319 5.69e-62 200
Msa0121960 MtrunA17_Chr7g0225221 37.383 321 184 7 12 322 7 320 3.07e-61 198
Msa0121960 MtrunA17_Chr3g0128061 34.557 327 204 5 5 323 3 327 5.44e-61 197
Msa0121960 MtrunA17_Chr7g0258811 36.778 329 194 8 5 323 48 372 6.79e-61 198
Msa0121960 MtrunA17_Chr5g0438021 34.084 311 200 4 14 322 18 325 2.17e-60 196
Msa0121960 MtrunA17_Chr6g0460711 34.921 315 193 5 12 322 8 314 2.82e-60 195
Msa0121960 MtrunA17_Chr6g0451571 36.335 322 192 6 10 322 17 334 4.86e-60 195
Msa0121960 MtrunA17_Chr6g0467121 36.145 332 186 9 9 323 42 364 1.74e-59 195
Msa0121960 MtrunA17_Chr5g0432441 35.873 315 193 5 11 322 18 326 4.22e-59 192
Msa0121960 MtrunA17_Chr4g0024121 34.756 328 202 5 4 322 2 326 1.51e-58 191
Msa0121960 MtrunA17_Chr1g0175561 35.294 323 194 8 12 324 11 328 4.93e-58 190
Msa0121960 MtrunA17_Chr4g0070951 34.524 336 201 9 1 323 1 330 8.08e-58 189
Msa0121960 MtrunA17_Chr3g0114451 35.423 319 195 6 10 322 5 318 1.18e-57 188
Msa0121960 MtrunA17_Chr2g0296621 39.474 304 166 8 27 323 50 342 2.35e-57 188
Msa0121960 MtrunA17_Chr2g0292241 37.942 311 177 6 25 324 27 332 2.49e-57 189
Msa0121960 MtrunA17_Chr5g0400331 35.849 318 188 5 18 322 17 331 4.54e-57 187
Msa0121960 MtrunA17_Chr6g0467251 35.593 295 181 5 27 318 36 324 6.10e-57 187
Msa0121960 MtrunA17_Chr2g0292211 36.111 324 194 6 10 324 24 343 7.67e-57 187
Msa0121960 MtrunA17_Chr2g0320071 34.891 321 193 7 11 322 4 317 8.45e-57 186
Msa0121960 MtrunA17_Chr1g0197411 33.636 330 195 8 8 318 2 326 1.10e-56 186
Msa0121960 MtrunA17_Chr2g0292151 35.599 309 187 5 25 324 27 332 1.23e-56 187
Msa0121960 MtrunA17_Chr6g0467161 34.441 331 195 8 9 324 15 338 1.50e-56 186
Msa0121960 MtrunA17_Chr4g0046131 35.197 304 184 6 26 318 41 342 1.90e-56 186
Msa0121960 MtrunA17_Chr3g0129061 34.505 313 197 5 9 319 13 319 2.36e-56 185
Msa0121960 MtrunA17_Chr8g0364291 36.646 322 194 4 9 322 5 324 3.41e-56 185
Msa0121960 MtrunA17_Chr2g0291751 36.893 309 183 5 25 324 27 332 4.80e-56 185
Msa0121960 MtrunA17_Chr2g0320031 34.551 301 189 5 25 322 24 319 4.85e-56 184
Msa0121960 MtrunA17_Chr2g0292181 36.570 309 184 5 25 324 36 341 9.18e-56 185
Msa0121960 MtrunA17_Chr1g0164891 35.294 306 184 6 28 322 90 392 2.08e-55 185
Msa0121960 MtrunA17_Chr7g0244461 31.847 314 206 5 12 322 4 312 2.20e-55 182
Msa0121960 MtrunA17_Chr4g0013821 33.766 308 189 6 25 324 26 326 3.65e-55 183
Msa0121960 MtrunA17_Chr2g0292251 36.039 308 185 5 25 323 26 330 5.25e-55 182
Msa0121960 MtrunA17_Chr2g0320061 33.439 314 198 6 12 322 13 318 5.91e-55 181
Msa0121960 MtrunA17_Chr1g0204011 33.333 324 204 5 5 322 5 322 9.99e-55 181
Msa0121960 MtrunA17_Chr2g0316791 33.435 329 184 7 25 318 74 402 2.24e-54 182
Msa0121960 MtrunA17_Chr1g0194151 35.065 308 188 6 25 322 28 333 2.80e-54 180
Msa0121960 MtrunA17_Chr4g0038171 33.025 324 204 6 6 322 4 321 4.69e-54 179
Msa0121960 MtrunA17_Chr2g0320051 33.663 303 189 6 25 322 23 318 5.71e-54 179
Msa0121960 MtrunA17_Chr2g0320101 33.968 315 194 6 12 322 13 317 6.39e-54 179
Msa0121960 MtrunA17_Chr4g0013721 33.876 307 190 5 25 324 98 398 1.12e-53 181
Msa0121960 MtrunA17_Chr4g0076211 35.880 301 176 6 29 322 43 333 1.40e-53 178
Msa0121960 MtrunA17_Chr4g0038151 32.166 314 210 3 11 322 9 321 1.82e-53 177
Msa0121960 MtrunA17_Chr2g0292111 34.628 309 190 5 25 324 27 332 2.71e-53 178
Msa0121960 MtrunA17_Chr5g0413051 34.628 309 191 7 24 322 70 377 2.80e-53 179
Msa0121960 MtrunA17_Chr3g0129041 33.974 312 201 4 13 322 14 322 2.90e-53 177
Msa0121960 MtrunA17_Chr2g0316191 32.727 330 186 7 25 318 74 403 3.28e-53 179
Msa0121960 MtrunA17_Chr4g0013201 34.106 302 188 5 25 322 44 338 4.50e-53 177
Msa0121960 MtrunA17_Chr4g0043391 33.439 314 197 5 16 322 16 324 6.06e-53 176
Msa0121960 MtrunA17_Chr2g0316481 32.827 329 186 7 25 318 74 402 7.65e-53 179
Msa0121960 MtrunA17_Chr1g0194271 35.350 314 186 6 25 322 28 340 1.21e-52 176
Msa0121960 MtrunA17_Chr2g0292091 34.194 310 192 5 23 323 20 326 1.51e-52 176
Msa0121960 MtrunA17_Chr1g0148021 35.312 320 194 6 12 322 13 328 2.81e-52 175
Msa0121960 MtrunA17_Chr2g0316831 32.827 329 186 7 25 318 110 438 3.61e-52 177
Msa0121960 MtrunA17_Chr4g0038161 32.166 314 209 4 11 322 9 320 3.73e-52 174
Msa0121960 MtrunA17_Chr2g0292261 34.824 313 186 6 24 324 26 332 4.37e-52 175
Msa0121960 MtrunA17_Chr2g0322861 34.783 322 198 6 9 322 69 386 5.33e-52 176
Msa0121960 MtrunA17_Chr2g0320091 32.278 316 206 5 11 322 7 318 5.97e-52 174
Msa0121960 MtrunA17_Chr4g0063671 33.437 323 196 7 12 322 5 320 9.64e-52 173
Msa0121960 MtrunA17_Chr1g0155611 37.785 307 175 6 27 322 46 347 2.21e-51 173
Msa0121960 MtrunA17_Chr7g0268501 33.841 328 190 9 10 322 5 320 2.31e-51 172
Msa0121960 MtrunA17_Chr7g0268521 33.841 328 190 9 10 322 5 320 2.31e-51 172
Msa0121960 MtrunA17_Chr2g0329641 33.550 307 192 5 24 322 33 335 5.07e-51 172
Msa0121960 MtrunA17_Chr5g0405991 36.364 319 185 9 13 322 7 316 5.44e-51 171
Msa0121960 MtrunA17_Chr4g0068621 34.539 304 190 6 25 322 36 336 5.76e-51 172
Msa0121960 MtrunA17_Chr4g0013171 35.430 302 186 6 25 322 25 321 8.81e-51 171
Msa0121960 MtrunA17_Chr1g0213061 32.399 321 205 8 9 323 8 322 1.74e-50 170
Msa0121960 MtrunA17_Chr2g0311251 31.098 328 192 5 25 318 9 336 1.78e-50 171
Msa0121960 MtrunA17_Chr7g0244451 32.166 314 202 5 13 322 10 316 5.87e-50 168
Msa0121960 MtrunA17_Chr6g0477071 31.457 302 198 4 22 322 49 342 6.39e-50 169
Msa0121960 MtrunA17_Chr2g0279041 31.818 330 203 7 8 322 3 325 7.24e-50 169
Msa0121960 MtrunA17_Chr3g0136751 32.923 325 203 5 7 322 4 322 8.20e-50 168
Msa0121960 MtrunA17_Chr4g0072091 34.437 302 184 6 26 318 23 319 9.37e-50 168
Msa0121960 MtrunA17_Chr4g0013191 34.323 303 189 6 25 322 43 340 1.44e-49 168
Msa0121960 MtrunA17_Chr5g0401431 34.579 321 197 5 11 321 11 328 2.04e-49 167
Msa0121960 MtrunA17_Chr8g0371381 34.768 302 183 6 27 322 26 319 3.31e-49 167
Msa0121960 MtrunA17_Chr2g0299691 34.098 305 184 6 31 323 60 359 3.42e-49 167
Msa0121960 MtrunA17_Chr2g0292101 32.609 322 203 6 12 324 12 328 1.11e-48 166
Msa0121960 MtrunA17_Chr2g0292291 33.766 308 193 5 24 322 27 332 1.87e-48 166
Msa0121960 MtrunA17_Chr5g0432351 34.049 326 198 9 8 322 2 321 2.80e-48 164
Msa0121960 MtrunA17_Chr1g0157051 31.481 324 193 7 12 321 9 317 3.18e-48 164
Msa0121960 MtrunA17_Chr3g0106901 33.229 319 202 5 8 322 12 323 1.74e-47 162
Msa0121960 MtrunA17_Chr1g0188431 31.650 297 194 4 27 322 39 327 1.94e-47 162
Msa0121960 MtrunA17_Chr2g0311241 28.649 370 187 7 25 318 58 426 6.92e-47 163
Msa0121960 MtrunA17_Chr4g0013181 34.680 297 185 6 30 322 62 353 9.34e-47 161
Msa0121960 MtrunA17_Chr2g0292271 32.692 312 194 6 24 324 26 332 1.05e-46 161
Msa0121960 MtrunA17_Chr3g0107881 31.790 324 203 9 10 322 8 324 2.05e-46 159
Msa0121960 MtrunA17_Chr5g0402571 33.229 319 202 6 11 322 12 326 3.33e-46 159
Msa0121960 MtrunA17_Chr7g0224371 31.761 318 204 7 13 323 12 323 5.09e-46 158
Msa0121960 MtrunA17_Chr5g0402581 32.602 319 204 6 11 322 12 326 1.70e-45 157
Msa0121960 MtrunA17_Chr5g0432331 33.007 306 190 8 25 322 54 352 2.94e-45 157
Msa0121960 MtrunA17_Chr5g0432341 33.443 305 190 8 25 322 23 321 6.69e-45 155
Msa0121960 MtrunA17_Chr4g0053871 33.445 299 191 4 25 318 25 320 1.58e-44 154
Msa0121960 MtrunA17_Chr2g0278671 32.278 316 184 8 24 322 21 323 9.94e-42 147
Msa0121960 MtrunA17_Chr5g0421411 32.509 283 167 8 57 323 12 286 1.17e-41 146
Msa0121960 MtrunA17_Chr2g0279061 31.179 263 165 5 69 322 11 266 1.62e-38 137
Msa0121960 MtrunA17_Chr2g0316811 34.211 228 123 5 25 227 48 273 3.02e-34 127
Msa0121960 MtrunA17_Chr4g0004591 25.397 315 173 5 27 318 77 352 5.32e-31 119
Msa0121960 MtrunA17_Chr5g0432401 27.607 326 172 10 10 321 10 285 7.64e-29 112
Msa0121960 MtrunA17_Chr2g0292281 32.864 213 123 4 118 323 18 217 8.92e-29 110
Msa0121960 MtrunA17_Chr2g0316141 27.562 283 161 7 72 323 11 280 6.41e-27 107
Msa0121960 MtrunA17_Chr2g0316181 27.562 283 161 7 72 323 11 280 6.41e-27 107
Msa0121960 MtrunA17_Chr3g0114461 33.161 193 123 3 131 322 5 192 1.48e-25 101
Msa0121960 MtrunA17_Chr3g0114491 42.857 112 62 2 23 132 14 125 1.50e-22 91.7
Msa0121960 MtrunA17_Chr6g0468731 45.205 73 37 1 65 137 22 91 5.85e-16 72.4
Msa0121960 MtrunA17_Chr7g0254181 36.036 111 67 2 97 206 31 138 4.45e-13 66.2
Msa0121960 MtrunA17_Chr5g0423911 40.909 66 39 0 253 318 2 67 2.16e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0121960 AT2G37130.1 69.836 305 90 2 20 322 23 327 1.09e-161 454
Msa0121960 AT2G37130.2 70.946 296 84 2 29 322 1 296 2.38e-158 444
Msa0121960 AT4G21960.1 53.872 297 136 1 27 322 31 327 3.96e-122 354
Msa0121960 AT4G08780.1 37.107 318 180 7 21 324 18 329 1.27e-68 218
Msa0121960 AT4G08770.1 37.072 321 182 7 18 324 15 329 5.93e-68 216
Msa0121960 AT3G50990.1 39.869 306 173 5 25 322 39 341 1.18e-64 207
Msa0121960 AT2G18150.1 36.202 337 192 6 6 322 3 336 2.16e-64 207
Msa0121960 AT5G17820.1 36.392 316 183 6 13 322 10 313 6.34e-64 205
Msa0121960 AT3G03670.1 35.625 320 197 3 9 322 5 321 7.56e-64 205
Msa0121960 AT3G17070.1 35.103 339 199 8 4 322 2 339 1.85e-63 204
Msa0121960 AT5G14130.1 36.562 320 192 4 10 322 15 330 3.62e-63 203
Msa0121960 AT5G14130.2 36.562 320 192 4 10 322 56 371 1.09e-62 203
Msa0121960 AT5G05340.1 35.216 301 190 4 24 322 27 324 1.16e-62 202
Msa0121960 AT5G17820.2 36.392 316 183 6 13 322 63 366 1.19e-62 203
Msa0121960 AT2G22420.1 34.365 323 197 6 10 324 6 321 9.35e-62 200
Msa0121960 AT3G32980.1 35.737 319 191 6 16 323 20 335 1.37e-61 200
Msa0121960 AT4G36430.1 36.877 301 179 6 31 323 34 331 2.36e-61 199
Msa0121960 AT2G18140.1 35.897 312 189 5 19 322 27 335 2.76e-61 199
Msa0121960 AT5G66390.1 37.000 300 178 6 31 322 37 333 2.92e-61 199
Msa0121960 AT3G49110.1 35.423 319 192 6 16 323 22 337 1.39e-60 197
Msa0121960 AT3G49120.1 35.714 322 187 7 16 323 21 336 1.45e-60 197
Msa0121960 AT5G06730.1 33.436 326 203 6 10 324 14 336 2.14e-60 197
Msa0121960 AT5G06720.1 35.030 334 202 8 3 324 5 335 7.12e-60 195
Msa0121960 AT1G30870.1 38.762 307 169 8 27 324 51 347 1.37e-58 192
Msa0121960 AT1G71695.1 34.106 302 191 4 27 324 44 341 5.36e-58 191
Msa0121960 AT4G16270.1 35.276 326 185 8 7 322 39 348 6.81e-58 190
Msa0121960 AT4G31760.1 34.675 323 196 5 9 322 10 326 7.41e-58 189
Msa0121960 AT4G33870.1 31.686 344 197 5 13 318 54 397 9.40e-58 192
Msa0121960 AT5G15180.1 35.276 326 194 8 9 322 9 329 2.64e-57 188
Msa0121960 AT2G39040.1 36.570 309 185 7 24 322 43 350 3.25e-57 189
Msa0121960 AT1G05260.1 34.091 308 191 6 24 323 23 326 4.25e-57 187
Msa0121960 AT5G40150.1 35.974 303 189 3 23 322 28 328 4.25e-57 187
Msa0121960 AT4G26010.1 35.556 315 188 6 10 322 8 309 6.00e-57 187
Msa0121960 AT1G34510.1 35.220 318 185 7 10 322 8 309 7.84e-57 186
Msa0121960 AT4G26010.2 35.556 315 188 6 10 322 36 337 1.31e-56 187
Msa0121960 AT1G14550.1 35.625 320 190 7 11 322 10 321 1.72e-56 186
Msa0121960 AT1G14540.1 35.016 317 196 6 12 322 3 315 2.96e-56 185
Msa0121960 AT5G24070.1 36.688 308 180 6 26 323 34 336 3.60e-55 183
Msa0121960 AT2G24800.1 34.202 307 187 6 25 322 29 329 5.18e-55 182
Msa0121960 AT1G24110.1 35.526 304 187 4 22 318 17 318 8.89e-55 182
Msa0121960 AT5G64120.1 32.343 303 193 5 25 322 33 328 9.08e-55 182
Msa0121960 AT2G35380.1 35.294 306 182 6 30 322 33 335 1.27e-54 181
Msa0121960 AT2G38390.1 33.856 319 198 6 16 324 20 335 7.54e-54 180
Msa0121960 AT2G41480.1 33.333 330 198 9 8 322 6 328 1.17e-53 179
Msa0121960 AT4G17690.1 35.452 299 188 3 27 322 26 322 1.91e-53 178
Msa0121960 AT3G01190.1 34.277 318 192 9 14 322 12 321 5.37e-53 177
Msa0121960 AT2G38380.1 33.856 319 198 6 16 324 20 335 6.57e-53 177
Msa0121960 AT1G05240.1 35.082 305 184 5 27 322 26 325 7.55e-53 177
Msa0121960 AT1G05250.1 35.082 305 184 5 27 322 26 325 7.55e-53 177
Msa0121960 AT5G58390.1 32.797 311 203 5 12 319 6 313 9.12e-53 176
Msa0121960 AT2G18980.1 33.731 335 193 6 4 322 2 323 2.66e-52 175
Msa0121960 AT5G19880.1 32.716 324 202 7 12 322 9 329 2.92e-52 175
Msa0121960 AT5G58400.1 32.099 324 205 8 4 319 6 322 5.33e-52 174
Msa0121960 AT2G34060.1 34.098 305 188 6 26 319 41 343 8.65e-52 174
Msa0121960 AT4G37530.1 33.866 313 192 6 21 322 21 329 2.22e-51 173
Msa0121960 AT4G11290.1 32.822 326 203 7 10 322 4 326 6.49e-51 172
Msa0121960 AT1G44970.1 36.893 309 179 6 25 322 43 346 7.67e-51 172
Msa0121960 AT4G37520.1 33.333 309 191 6 25 322 25 329 1.02e-50 171
Msa0121960 AT1G49570.1 33.550 307 189 6 25 320 46 348 1.12e-50 172
Msa0121960 AT1G68850.1 33.010 309 193 8 27 323 30 336 1.59e-50 171
Msa0121960 AT3G28200.1 33.993 303 194 5 23 322 17 316 3.17e-50 169
Msa0121960 AT3G17070.2 37.165 261 156 6 68 322 18 276 3.48e-50 168
Msa0121960 AT4G33420.1 32.063 315 205 4 9 322 19 325 4.90e-50 169
Msa0121960 AT5G51890.1 33.007 306 192 6 24 322 23 322 7.33e-50 169
Msa0121960 AT4G30170.1 33.987 306 189 5 25 322 25 325 8.34e-50 169
Msa0121960 AT5G42180.1 32.508 323 201 7 8 321 2 316 1.65e-49 167
Msa0121960 AT4G33420.2 32.063 315 205 4 9 322 66 372 1.98e-49 169
Msa0121960 AT3G21770.1 33.119 311 196 6 16 318 18 324 2.10e-49 168
Msa0121960 AT5G39580.1 32.226 301 191 6 28 322 26 319 6.56e-49 166
Msa0121960 AT5G47000.1 34.333 300 191 4 27 322 33 330 1.11e-48 166
Msa0121960 AT4G37520.2 33.333 309 188 7 25 322 25 326 2.08e-48 165
Msa0121960 AT5G22410.1 32.915 319 197 9 11 319 11 322 8.27e-48 164
Msa0121960 AT2G43480.1 32.573 307 192 6 26 322 34 335 3.16e-47 162
Msa0121960 AT5G19890.1 32.903 310 188 8 24 322 27 327 2.84e-46 159
Msa0121960 AT1G77100.1 32.500 320 201 9 12 322 6 319 6.78e-46 158
Msa0121960 AT4G25980.1 31.250 320 201 8 15 322 14 326 3.33e-45 157
Msa0121960 AT5G67400.1 33.657 309 190 6 25 322 25 329 7.47e-44 153
Msa0121960 AT4G37530.2 34.848 264 157 6 21 273 21 280 2.79e-43 150
Msa0121960 AT3G49960.1 31.776 321 206 5 12 322 12 329 6.05e-43 151
Msa0121960 AT2G35380.2 34.177 237 142 4 97 322 14 247 4.45e-40 141
Msa0121960 AT5G64110.1 31.683 303 188 7 30 322 37 330 1.22e-38 139
Msa0121960 AT5G64100.1 29.085 306 194 7 28 322 38 331 1.14e-34 129
Msa0121960 AT4G25980.2 36.111 180 109 4 15 192 59 234 1.48e-29 114
Msa0121960 AT2G24800.2 34.118 170 108 2 25 193 29 195 2.85e-26 104
Msa0121960 AT5G39580.2 32.022 178 116 3 28 204 26 199 1.46e-24 100
Msa0121960 AT3G42570.1 37.143 105 65 1 87 190 43 147 3.71e-16 75.1

Find 78 sgRNAs with CRISPR-Local

Find 211 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAGAGAATAATCCTCTTAT+TGG 0.181658 1_3:+48210282 Msa0121960:CDS
TGTACATAAGGTGATGTTAT+TGG 0.218584 1_3:-48210198 None:intergenic
CAGACATCGGTAAGAAGTTT+TGG 0.224300 1_3:+48207927 Msa0121960:CDS
AGGCTAATTATATATCTATT+TGG 0.226220 1_3:-48210346 None:intergenic
CCACTCTTCATTTCAATGTT+TGG 0.239217 1_3:-48209686 None:intergenic
TTCTAGATATATGTTGTAAA+AGG 0.244000 1_3:+48210508 Msa0121960:three_prime_UTR
TTTGCCTTTGGATTTGGTTT+TGG 0.250284 1_3:-48210057 None:intergenic
TTGATTATCTCTTCGGCTCT+TGG 0.275811 1_3:-48207689 None:intergenic
TTTCTCAACAGCAGCTTTGA+TGG 0.281307 1_3:-48207976 None:intergenic
TATACTGTATTAGTTGGTTT+TGG 0.284893 1_3:+48210541 Msa0121960:three_prime_UTR
CTCACTCGTTTCCAAGCTAT+TGG 0.285653 1_3:+48209788 Msa0121960:CDS
GAATAAGTAAATTATTTCTA+TGG 0.294071 1_3:-48210421 None:intergenic
CCAAGGACATGGAGGCATTA+TGG 0.297639 1_3:-48209763 None:intergenic
AAGCAATAGAAAGAGGAAAT+AGG 0.306631 1_3:-48207126 None:intergenic
GTACATAAGGTGATGTTATT+GGG 0.332380 1_3:-48210197 None:intergenic
ACATTTAAACTTGCTAGATA+TGG 0.336546 1_3:+48207082 Msa0121960:exon
CTCTTCGGCTCTTGGGCAGC+TGG 0.364309 1_3:-48207681 None:intergenic
TATCGAATTGTAGAGATGTT+TGG 0.366067 1_3:+48210454 Msa0121960:three_prime_UTR
TCACCTTGATCTCCAATAAG+AGG 0.374831 1_3:-48210294 None:intergenic
CTACTCTATACTGTATTAGT+TGG 0.385455 1_3:+48210535 Msa0121960:three_prime_UTR
ACAAATACAAACTATATTGT+AGG 0.385763 1_3:-48210366 None:intergenic
TTGTTGCTTGATTATCTCTT+CGG 0.386543 1_3:-48207696 None:intergenic
GTCTAGCGTTGGATCCACTA+TGG 0.388158 1_3:-48210001 None:intergenic
AAGAGTACACTGCATAAATA+TGG 0.396458 1_3:+48209963 Msa0121960:CDS
GATATTTCTATTTAGTAAGA+TGG 0.421253 1_3:-48210576 None:intergenic
GACATGGAGGCATTATGGTT+TGG 0.428570 1_3:-48209758 None:intergenic
TGATTATCTCTTCGGCTCTT+GGG 0.432566 1_3:-48207688 None:intergenic
TTGAAATGAAGAGTGGAAGA+AGG 0.466644 1_3:+48209693 Msa0121960:CDS
TTTAGGTAGGAAACATATGT+AGG 0.471391 1_3:-48210024 None:intergenic
AATCCTCTTATTGGAGATCA+AGG 0.479309 1_3:+48210291 Msa0121960:CDS
CCATAATGCCTCCATGTCCT+TGG 0.488227 1_3:+48209763 Msa0121960:CDS
AACGTGGCGTGGTGTGGTAT+CGG 0.489059 1_3:+48207902 Msa0121960:CDS
TCACATTGACCATGCAATCA+TGG 0.491944 1_3:-48207775 None:intergenic
TTCGGCTCTTGGGCAGCTGG+TGG 0.494213 1_3:-48207678 None:intergenic
GAGATTTCGAAGCCACGAAA+TGG 0.495427 1_3:-48207750 None:intergenic
AGTAGTAGTTCAGCTGGAGC+TGG 0.495513 1_3:-48207655 None:intergenic
TGGTGGAGTAGTAGTTCAGC+TGG 0.496466 1_3:-48207661 None:intergenic
GGAGGCATTATGGTTTGGAA+TGG 0.501790 1_3:-48209753 None:intergenic
AAGGAAGCACAGCCATTTCG+TGG 0.505711 1_3:+48207738 Msa0121960:CDS
TAGTTGTTATCAATTATCAA+TGG 0.505973 1_3:-48210114 None:intergenic
AGATCAAGGTGAAATTAGAA+AGG 0.511339 1_3:+48210305 Msa0121960:CDS
TGAAATGAAGAGTGGAAGAA+GGG 0.514033 1_3:+48209694 Msa0121960:CDS
TTGGATAATCCTCAAAATGA+AGG 0.514386 1_3:+48210473 Msa0121960:three_prime_UTR
GTCACTGAACTATATAATCA+AGG 0.517848 1_3:+48207719 Msa0121960:CDS
GTAGGATAAGCAATAGAAAG+AGG 0.523449 1_3:-48207133 None:intergenic
TCTAGCGTTGGATCCACTAT+GGG 0.531201 1_3:-48210000 None:intergenic
TTTAGCAACGTGGCGTGGTG+TGG 0.538261 1_3:+48207896 Msa0121960:CDS
TGCTAGAGACGCCATTGCAA+TGG 0.547486 1_3:+48208043 Msa0121960:CDS
CAACATCAACACCAATAGCT+TGG 0.551491 1_3:-48209799 None:intergenic
ATATACCCAAGTTAAAATGT+AGG 0.552543 1_3:-48207151 None:intergenic
TAGCAAAGAAAGCTATGTAA+AGG 0.556477 1_3:+48209718 Msa0121960:CDS
AAATCTCTTCCATGATTGCA+TGG 0.571041 1_3:+48207766 Msa0121960:CDS
TTAGGTAGGAAACATATGTA+GGG 0.579127 1_3:-48210023 None:intergenic
AGAAAGCTATGTAAAGGTGG+TGG 0.581012 1_3:+48209724 Msa0121960:CDS
CAAAGAAAGCTATGTAAAGG+TGG 0.592533 1_3:+48209721 Msa0121960:CDS
ATCAGCACATGATACTGTCA+AGG 0.596717 1_3:-48208006 None:intergenic
CCAAACATTGAAATGAAGAG+TGG 0.598378 1_3:+48209686 Msa0121960:CDS
CCACTATGGGGTATAGTCTG+TGG 0.598392 1_3:-48209987 None:intergenic
AGGTGATGTTATTGGGTCAG+TGG 0.598487 1_3:-48210190 None:intergenic
ACGAGTGAGAACCAAGGACA+TGG 0.603572 1_3:-48209774 None:intergenic
CCACAGACTATACCCCATAG+TGG 0.603814 1_3:+48209987 Msa0121960:CDS
TGAATCAGGAGCACATTCAG+TGG 0.611989 1_3:+48209939 Msa0121960:intron
ACTTCAATGAGCAATTCTCA+AGG 0.615505 1_3:+48210244 Msa0121960:CDS
TGGTGTTGATGTTGAAGCCA+CGG 0.619561 1_3:+48209808 Msa0121960:CDS
ATGTCCAAAACCAAATCCAA+AGG 0.620759 1_3:+48210053 Msa0121960:CDS
ACATATGTAGGGTCTAGCGT+TGG 0.622105 1_3:-48210012 None:intergenic
GAATCAGGAGCACATTCAGT+GGG 0.623327 1_3:+48209940 Msa0121960:intron
GCATAGACATACCATTGCAA+TGG 0.626620 1_3:-48208054 None:intergenic
CTTCAATGAGCAATTCTCAA+GGG 0.627350 1_3:+48210245 Msa0121960:CDS
TGTGGTATCGGAGCAGACAT+CGG 0.648826 1_3:+48207914 Msa0121960:CDS
CAAAAGCTGTCGAATATGTG+AGG 0.654580 1_3:+48210076 Msa0121960:CDS
CTAGCGTTGGATCCACTATG+GGG 0.672364 1_3:-48209999 None:intergenic
GATACCTAAAAGAGCAACCG+TGG 0.673209 1_3:-48209825 None:intergenic
TCAGCACATGATACTGTCAA+GGG 0.681224 1_3:-48208005 None:intergenic
AGGAAATAGGAATTAGAACG+AGG 0.699200 1_3:-48207113 None:intergenic
GCATAAGGGTCTTCTCACTG+TGG 0.719016 1_3:+48210155 Msa0121960:CDS
AGTGAGAACCAAGGACATGG+AGG 0.728213 1_3:-48209771 None:intergenic
TTGGAAACGAGTGAGAACCA+AGG 0.778312 1_3:-48209780 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTTTTTTTTTTTAAA+TGG + chr1_3:48207328-48207347 Msa0121960:intron 0.0%
!!! TTTTTTTTTTTTTTTTAAAT+GGG + chr1_3:48207329-48207348 Msa0121960:intron 0.0%
!! AAAAAAAAAATTAAAAAAAT+TGG - chr1_3:48208163-48208182 None:intergenic 0.0%
!! AAAAAAAAAAAAAAAAACCA+AGG - chr1_3:48207325-48207344 None:intergenic 10.0%
!!! AAAAACATTATAAACTTTTT+TGG + chr1_3:48208464-48208483 Msa0121960:intron 10.0%
!!! ATGTATTAACATTTTTTTTT+GGG - chr1_3:48209016-48209035 None:intergenic 10.0%
!!! TATGTATTAACATTTTTTTT+TGG - chr1_3:48209017-48209036 None:intergenic 10.0%
!!! AAAATATTTAATTTGCATTT+TGG + chr1_3:48209265-48209284 Msa0121960:intron 10.0%
!! AATAAAATGTTTATGTAAAT+TGG - chr1_3:48209903-48209922 None:intergenic 10.0%
!!! TACATAAACATTTTATTAAT+TGG + chr1_3:48209906-48209925 Msa0121960:intron 10.0%
!!! GTATATCTTTATAATTTTGT+AGG + chr1_3:48207359-48207378 Msa0121960:intron 15.0%
!! TTAAAACAGAAAAAATGAAA+GGG - chr1_3:48208268-48208287 None:intergenic 15.0%
!!! TTTAAAACAGAAAAAATGAA+AGG - chr1_3:48208269-48208288 None:intergenic 15.0%
!!! TTTTGCAATTTTATATCAAT+AGG - chr1_3:48208404-48208423 None:intergenic 15.0%
!! TACTAATTAAATAGTTAAGA+GGG - chr1_3:48208508-48208527 None:intergenic 15.0%
!! ATACTAATTAAATAGTTAAG+AGG - chr1_3:48208834-48208853 None:intergenic 15.0%
!!! TGATATTATTGATTAGAATA+TGG + chr1_3:48208923-48208942 Msa0121960:intron 15.0%
!! ATATTAATATTAGACAAACA+CGG - chr1_3:48209205-48209224 None:intergenic 15.0%
!! CAAATATATACAGTTTAATT+TGG - chr1_3:48209306-48209325 None:intergenic 15.0%
!! CAAATTAAACTGTATATATT+TGG + chr1_3:48209304-48209323 Msa0121960:intron 15.0%
!!! GAATAAGTAAATTATTTCTA+TGG - chr1_3:48210424-48210443 None:intergenic 15.0%
!! AAATATTGGTCACAAAAAAA+AGG - chr1_3:48207204-48207223 None:intergenic 20.0%
!! AAAAAAAAAAAAAACCAAGG+TGG - chr1_3:48207322-48207341 None:intergenic 20.0%
!! TAAAACAGAAAAAATGAAAG+GGG - chr1_3:48208267-48208286 None:intergenic 20.0%
!!! CGTTTTTGAATTAAAACTAA+CGG - chr1_3:48208736-48208755 None:intergenic 20.0%
!!! AGTTTTAATTCAAAAACGTT+AGG + chr1_3:48208609-48208628 Msa0121960:intron 20.0%
!!! GTTTTAATTCAAAAACGTTA+GGG + chr1_3:48208610-48208629 Msa0121960:intron 20.0%
!!! GTTTTAATTCAAAAACGTTA+GGG - chr1_3:48208610-48208629 None:intergenic 20.0%
!!! AGTTTTAATTCAAAAACGTT+AGG - chr1_3:48208609-48208628 None:intergenic 20.0%
!!! TGAATATTTTATACGAGTAT+GGG - chr1_3:48208888-48208907 None:intergenic 20.0%
!!! ATGAATATTTTATACGAGTA+TGG - chr1_3:48208889-48208908 None:intergenic 20.0%
!! TGTATTAACATATACTTATG+CGG - chr1_3:48208985-48209004 None:intergenic 20.0%
!! TAAACTGTATATATTTGGAT+CGG + chr1_3:48209309-48209328 Msa0121960:intron 20.0%
!! AATAAAAATCACATGCAATT+TGG - chr1_3:48209374-48209393 None:intergenic 20.0%
!!! ATTGCATGTGATTTTTATTT+TGG + chr1_3:48209375-48209394 Msa0121960:intron 20.0%
!! AGACTAATCTATTAATTAGA+AGG - chr1_3:48209525-48209544 None:intergenic 20.0%
!!! TAATAGATTAGTCTTTAGAT+TGG + chr1_3:48209531-48209550 Msa0121960:intron 20.0%
!!! TCTTTATTTTCACATAGTTA+AGG + chr1_3:48209569-48209588 Msa0121960:intron 20.0%
!! TAGTTGTTATCAATTATCAA+TGG - chr1_3:48210117-48210136 None:intergenic 20.0%
!! AGGCTAATTATATATCTATT+TGG - chr1_3:48210349-48210368 None:intergenic 20.0%
!! ACAAATACAAACTATATTGT+AGG - chr1_3:48210369-48210388 None:intergenic 20.0%
!! TTCTAGATATATGTTGTAAA+AGG + chr1_3:48210508-48210527 Msa0121960:three_prime_UTR 20.0%
!!! ACATTTAAACTTGCTAGATA+TGG + chr1_3:48207082-48207101 Msa0121960:exon 25.0%
!!! CTTATCCTACATTTTAACTT+GGG + chr1_3:48207146-48207165 Msa0121960:CDS 25.0%
! ATATACCCAAGTTAAAATGT+AGG - chr1_3:48207154-48207173 None:intergenic 25.0%
!!! AGTCATGTATTTTTATGTAC+AGG + chr1_3:48207626-48207645 Msa0121960:intron 25.0%
!!! GTCATGTATTTTTATGTACA+GGG + chr1_3:48207627-48207646 Msa0121960:intron 25.0%
!!! AATTTTTTTTCCAACATGAG+AGG + chr1_3:48208287-48208306 Msa0121960:intron 25.0%
!!! ATTTTTTTTCCAACATGAGA+GGG + chr1_3:48208288-48208307 Msa0121960:intron 25.0%
! GCCTACTTTATATGTATATA+AGG + chr1_3:48208322-48208341 Msa0121960:intron 25.0%
! GCCTTATATACATATAAAGT+AGG - chr1_3:48208326-48208345 None:intergenic 25.0%
! CTATTTAATTAGTATCGCTT+TGG + chr1_3:48208514-48208533 Msa0121960:intron 25.0%
!!! ATTTTCTTCTGGATTTTTTC+TGG + chr1_3:48208640-48208659 Msa0121960:intron 25.0%
!!! TTTTCTTCTGGATTTTTTCT+GGG + chr1_3:48208641-48208660 Msa0121960:intron 25.0%
! TAAATGACCAAAATGTGTAA+CGG + chr1_3:48208758-48208777 Msa0121960:intron 25.0%
! CTATTTAATTAGTATCGCTT+TGG - chr1_3:48208514-48208533 None:intergenic 25.0%
!!! AGTTTAATTTGGATCGATTT+TGG - chr1_3:48209295-48209314 None:intergenic 25.0%
! GCTTTCATTGTAAAAATTAG+AGG - chr1_3:48209449-48209468 None:intergenic 25.0%
!! CTAATTTTTACAATGAAAGC+CGG + chr1_3:48209449-48209468 Msa0121960:intron 25.0%
!! ATTCATTTGACTTATAAGCA+GGG - chr1_3:48209500-48209519 None:intergenic 25.0%
! TACTATGTTGATGTTAATGT+TGG + chr1_3:48209624-48209643 Msa0121960:intron 25.0%
!! TGCGTTTTGTATATTTAGTT+AGG + chr1_3:48209659-48209678 Msa0121960:intron 25.0%
! ATCACCTTATGTACAAAAAA+TGG + chr1_3:48210206-48210225 Msa0121960:CDS 25.0%
!!! AATTTGTTTCCTTCATTTTG+AGG - chr1_3:48210485-48210504 None:intergenic 25.0%
!!! TATACTGTATTAGTTGGTTT+TGG + chr1_3:48210541-48210560 Msa0121960:three_prime_UTR 25.0%
AAGCAATAGAAAGAGGAAAT+AGG - chr1_3:48207129-48207148 None:intergenic 30.0%
!!! GCTTATCCTACATTTTAACT+TGG + chr1_3:48207145-48207164 Msa0121960:CDS 30.0%
! GTATGTATAAATTTTGCCAC+AGG - chr1_3:48207232-48207251 None:intergenic 30.0%
ATATTATAGAGTTACCACCT+TGG + chr1_3:48207305-48207324 Msa0121960:intron 30.0%
! GACTTGATCATTTCTTTTCA+AGG + chr1_3:48207381-48207400 Msa0121960:intron 30.0%
! TTTTCACTTAAACGCAAAGT+AGG + chr1_3:48207417-48207436 Msa0121960:intron 30.0%
TAATGATAAGAATCTCGTGT+TGG - chr1_3:48207471-48207490 None:intergenic 30.0%
! TTGTTGCTTGATTATCTCTT+CGG - chr1_3:48207699-48207718 None:intergenic 30.0%
GTCACTGAACTATATAATCA+AGG + chr1_3:48207719-48207738 Msa0121960:CDS 30.0%
TTGAAACGATATAACTGTTG+TGG - chr1_3:48207821-48207840 None:intergenic 30.0%
! GATCTATTTGCATCCTTTTA+GGG - chr1_3:48208110-48208129 None:intergenic 30.0%
! TGATCTATTTGCATCCTTTT+AGG - chr1_3:48208111-48208130 None:intergenic 30.0%
ATCAATAGGTTAAAGAGAGT+TGG - chr1_3:48208390-48208409 None:intergenic 30.0%
! TTTCTCTCTGTTACACATTT+TGG + chr1_3:48208575-48208594 Msa0121960:intron 30.0%
ATGATATGAAGATGAAGAAG+AGG + chr1_3:48208690-48208709 Msa0121960:intron 30.0%
! AGTTGGCTAAATTGCATTTT+TGG - chr1_3:48208860-48208879 None:intergenic 30.0%
TGCATTATTATGTTGCTAGT+TGG + chr1_3:48209065-48209084 Msa0121960:intron 30.0%
GACAAACACGGTAAAATAAT+AGG - chr1_3:48209193-48209212 None:intergenic 30.0%
TGTATATATTTGGATCGGAT+CGG + chr1_3:48209314-48209333 Msa0121960:intron 30.0%
AAATCACATGCAATTTGGTT+CGG - chr1_3:48209369-48209388 None:intergenic 30.0%
! GATTCATTTGACTTATAAGC+AGG - chr1_3:48209501-48209520 None:intergenic 30.0%
GTTAATGTTGGATTGTTTGT+TGG + chr1_3:48209636-48209655 Msa0121960:intron 30.0%
TAGCAAAGAAAGCTATGTAA+AGG + chr1_3:48209718-48209737 Msa0121960:CDS 30.0%
TAGGTATCAAACATTCTTCT+TGG + chr1_3:48209840-48209859 Msa0121960:intron 30.0%
AAACATTCTTCTTGGCTAAT+TGG + chr1_3:48209848-48209867 Msa0121960:intron 30.0%
! AAGAGTACACTGCATAAATA+TGG + chr1_3:48209963-48209982 Msa0121960:CDS 30.0%
TTAGGTAGGAAACATATGTA+GGG - chr1_3:48210026-48210045 None:intergenic 30.0%
TTTAGGTAGGAAACATATGT+AGG - chr1_3:48210027-48210046 None:intergenic 30.0%
! AAGAACATTTTGCAGCATAA+GGG + chr1_3:48210141-48210160 Msa0121960:CDS 30.0%
GTACATAAGGTGATGTTATT+GGG - chr1_3:48210200-48210219 None:intergenic 30.0%
TGTACATAAGGTGATGTTAT+TGG - chr1_3:48210201-48210220 None:intergenic 30.0%
!!! TCTGCCATTTTTTGTACATA+AGG - chr1_3:48210213-48210232 None:intergenic 30.0%
CAAAAAATGGCAGATGATAA+TGG + chr1_3:48210219-48210238 Msa0121960:CDS 30.0%
TCAGAGAATAATCCTCTTAT+TGG + chr1_3:48210282-48210301 Msa0121960:CDS 30.0%
AGATCAAGGTGAAATTAGAA+AGG + chr1_3:48210305-48210324 Msa0121960:CDS 30.0%
TATCGAATTGTAGAGATGTT+TGG + chr1_3:48210454-48210473 Msa0121960:three_prime_UTR 30.0%
TTGGATAATCCTCAAAATGA+AGG + chr1_3:48210473-48210492 Msa0121960:three_prime_UTR 30.0%
CTACTCTATACTGTATTAGT+TGG + chr1_3:48210535-48210554 Msa0121960:three_prime_UTR 30.0%
AGGAAATAGGAATTAGAACG+AGG - chr1_3:48207116-48207135 None:intergenic 35.0%
GTAGGATAAGCAATAGAAAG+AGG - chr1_3:48207136-48207155 None:intergenic 35.0%
GACCAATATTTAAAGACCTG+TGG + chr1_3:48207213-48207232 Msa0121960:intron 35.0%
TGCCACAGGTCTTTAAATAT+TGG - chr1_3:48207218-48207237 None:intergenic 35.0%
! TGATAACCTACTTTGCGTTT+TGG - chr1_3:48207446-48207465 None:intergenic 35.0%
AAATCTCTTCCATGATTGCA+TGG + chr1_3:48207766-48207785 Msa0121960:CDS 35.0%
! AGACATCGGTAAGAAGTTTT+GGG + chr1_3:48207928-48207947 Msa0121960:CDS 35.0%
TGCCATCATATACACATGTT+AGG + chr1_3:48208074-48208093 Msa0121960:intron 35.0%
CTCCTAACATGTGTATATGA+TGG - chr1_3:48208079-48208098 None:intergenic 35.0%
!! TATTTGCATCCTTTTAGGGA+CGG - chr1_3:48208106-48208125 None:intergenic 35.0%
! AAAATTGGACCCCCAAATTT+TGG - chr1_3:48208148-48208167 None:intergenic 35.0%
AGAGAGTTGGATTGAATTGA+AGG - chr1_3:48208377-48208396 None:intergenic 35.0%
! AGGGTTTCTTCATTTTCTTC+TGG + chr1_3:48208629-48208648 Msa0121960:intron 35.0%
!!! CTTTTGTTGTTGAAGGTTGA+TGG + chr1_3:48208665-48208684 Msa0121960:intron 35.0%
! TTTCTCTCCGTTACACATTT+TGG - chr1_3:48208768-48208787 None:intergenic 35.0%
TACGAGTATGGGAAAAAAGT+TGG - chr1_3:48208877-48208896 None:intergenic 35.0%
TAACATATACTTATGCGGAG+CGG - chr1_3:48208980-48208999 None:intergenic 35.0%
CCACTCTTCATTTCAATGTT+TGG - chr1_3:48209689-48209708 None:intergenic 35.0%
CCAAACATTGAAATGAAGAG+TGG + chr1_3:48209686-48209705 Msa0121960:CDS 35.0%
TTGAAATGAAGAGTGGAAGA+AGG + chr1_3:48209693-48209712 Msa0121960:CDS 35.0%
TGAAATGAAGAGTGGAAGAA+GGG + chr1_3:48209694-48209713 Msa0121960:CDS 35.0%
CAAAGAAAGCTATGTAAAGG+TGG + chr1_3:48209721-48209740 Msa0121960:CDS 35.0%
! TTGGTCACTTTGATTGAATC+AGG + chr1_3:48209925-48209944 Msa0121960:intron 35.0%
!! GTTTTGGACATCTACGTTTT+AGG - chr1_3:48210044-48210063 None:intergenic 35.0%
ATGTCCAAAACCAAATCCAA+AGG + chr1_3:48210053-48210072 Msa0121960:CDS 35.0%
!! TTTGCCTTTGGATTTGGTTT+TGG - chr1_3:48210060-48210079 None:intergenic 35.0%
! CAAGAACATTTTGCAGCATA+AGG + chr1_3:48210140-48210159 Msa0121960:CDS 35.0%
ACTTCAATGAGCAATTCTCA+AGG + chr1_3:48210244-48210263 Msa0121960:CDS 35.0%
CTTCAATGAGCAATTCTCAA+GGG + chr1_3:48210245-48210264 Msa0121960:CDS 35.0%
AATCCTCTTATTGGAGATCA+AGG + chr1_3:48210291-48210310 Msa0121960:CDS 35.0%
AGGTTACCAAAACGCAAAGT+AGG + chr1_3:48207437-48207456 Msa0121960:intron 40.0%
TGATTATCTCTTCGGCTCTT+GGG - chr1_3:48207691-48207710 None:intergenic 40.0%
TTGATTATCTCTTCGGCTCT+TGG - chr1_3:48207692-48207711 None:intergenic 40.0%
TCACATTGACCATGCAATCA+TGG - chr1_3:48207778-48207797 None:intergenic 40.0%
! CATCGTTTCTTTTAGCAACG+TGG + chr1_3:48207886-48207905 Msa0121960:CDS 40.0%
! CAGACATCGGTAAGAAGTTT+TGG + chr1_3:48207927-48207946 Msa0121960:CDS 40.0%
! TTTCTCAACAGCAGCTTTGA+TGG - chr1_3:48207979-48207998 None:intergenic 40.0%
TCAGCACATGATACTGTCAA+GGG - chr1_3:48208008-48208027 None:intergenic 40.0%
ATCAGCACATGATACTGTCA+AGG - chr1_3:48208009-48208028 None:intergenic 40.0%
GCATAGACATACCATTGCAA+TGG - chr1_3:48208057-48208076 None:intergenic 40.0%
!! ATTTGCATCCTTTTAGGGAC+GGG - chr1_3:48208105-48208124 None:intergenic 40.0%
! CAAATTTTGGATTCCCAGCT+CGG - chr1_3:48208135-48208154 None:intergenic 40.0%
GAGCTGGGAATCCAAAATTT+GGG + chr1_3:48208134-48208153 Msa0121960:intron 40.0%
AGCTGGGAATCCAAAATTTG+GGG + chr1_3:48208135-48208154 Msa0121960:intron 40.0%
! TGCGAGAAGCATATCATATC+AGG + chr1_3:48208225-48208244 Msa0121960:intron 40.0%
!!! TCTGGGACTTTTGTTGTTGA+AGG + chr1_3:48208658-48208677 Msa0121960:intron 40.0%
!! TGTGGTTTAGATCGGTTTTG+AGG - chr1_3:48209417-48209436 None:intergenic 40.0%
GTTTGCGATGTGGTTTAGAT+CGG - chr1_3:48209425-48209444 None:intergenic 40.0%
!! AATTAGAGGTGTTTGCGATG+TGG - chr1_3:48209435-48209454 None:intergenic 40.0%
TTCCTGTGAAATTGGAGATC+CGG - chr1_3:48209471-48209490 None:intergenic 40.0%
! CATTTGACTTATAAGCAGGG+CGG - chr1_3:48209497-48209516 None:intergenic 40.0%
AGAAAGCTATGTAAAGGTGG+TGG + chr1_3:48209724-48209743 Msa0121960:CDS 40.0%
CAACATCAACACCAATAGCT+TGG - chr1_3:48209802-48209821 None:intergenic 40.0%
!! TGGACATCTACGTTTTAGGT+AGG - chr1_3:48210040-48210059 None:intergenic 40.0%
! ACAGCTTTTGCCTTTGGATT+TGG - chr1_3:48210066-48210085 None:intergenic 40.0%
! TATTCGACAGCTTTTGCCTT+TGG - chr1_3:48210072-48210091 None:intergenic 40.0%
CAAAAGCTGTCGAATATGTG+AGG + chr1_3:48210076-48210095 Msa0121960:CDS 40.0%
TCACCTTGATCTCCAATAAG+AGG - chr1_3:48210297-48210316 None:intergenic 40.0%
ATCATGTGATCCGTCTAACC+AGG + chr1_3:48207512-48207531 Msa0121960:intron 45.0%
GAGATTTCGAAGCCACGAAA+TGG - chr1_3:48207753-48207772 None:intergenic 45.0%
! TTTCTTTTAGCAACGTGGCG+TGG + chr1_3:48207891-48207910 Msa0121960:CDS 45.0%
CGAGCTGGGAATCCAAAATT+TGG + chr1_3:48208133-48208152 Msa0121960:intron 45.0%
GCTGGGAATCCAAAATTTGG+GGG + chr1_3:48208136-48208155 Msa0121960:intron 45.0%
TTTCCAACATGAGAGGGACT+TGG + chr1_3:48208294-48208313 Msa0121960:intron 45.0%
AAACCAAGTCCCTCTCATGT+TGG - chr1_3:48208300-48208319 None:intergenic 45.0%
!! ACATGAGAGGGACTTGGTTT+TGG + chr1_3:48208300-48208319 Msa0121960:intron 45.0%
TATACTTATGCGGAGCGGTT+CGG - chr1_3:48208975-48208994 None:intergenic 45.0%
!! GGAGGCATTATGGTTTGGAA+TGG - chr1_3:48209756-48209775 None:intergenic 45.0%
!! GACATGGAGGCATTATGGTT+TGG - chr1_3:48209761-48209780 None:intergenic 45.0%
TTGGAAACGAGTGAGAACCA+AGG - chr1_3:48209783-48209802 None:intergenic 45.0%
CTCACTCGTTTCCAAGCTAT+TGG + chr1_3:48209788-48209807 Msa0121960:CDS 45.0%
!! TGGTGTTGATGTTGAAGCCA+CGG + chr1_3:48209808-48209827 Msa0121960:CDS 45.0%
GATACCTAAAAGAGCAACCG+TGG - chr1_3:48209828-48209847 None:intergenic 45.0%
! TGAATCAGGAGCACATTCAG+TGG + chr1_3:48209939-48209958 Msa0121960:intron 45.0%
! GAATCAGGAGCACATTCAGT+GGG + chr1_3:48209940-48209959 Msa0121960:intron 45.0%
! TCTAGCGTTGGATCCACTAT+GGG - chr1_3:48210003-48210022 None:intergenic 45.0%
ACATATGTAGGGTCTAGCGT+TGG - chr1_3:48210015-48210034 None:intergenic 45.0%
AGGTGATGTTATTGGGTCAG+TGG - chr1_3:48210193-48210212 None:intergenic 45.0%
!!! AATAAATCAAATTTTAATTA+AGG - chr1_3:48208801-48208820 None:intergenic 5.0%
!!! AATTAAAATTTGATTTATTT+TGG - chr1_3:48208543-48208562 None:intergenic 5.0%
!!! AATAAATCAAATTTTAATTA+AGG + chr1_3:48208801-48208820 Msa0121960:intron 5.0%
GTTGTCTGCTCCTGGTTAGA+CGG - chr1_3:48207525-48207544 None:intergenic 50.0%
GAAAGTGAGTTGTCTGCTCC+TGG - chr1_3:48207533-48207552 None:intergenic 50.0%
AGTAGTAGTTCAGCTGGAGC+TGG - chr1_3:48207658-48207677 None:intergenic 50.0%
TGGTGGAGTAGTAGTTCAGC+TGG - chr1_3:48207664-48207683 None:intergenic 50.0%
! AAGGAAGCACAGCCATTTCG+TGG + chr1_3:48207738-48207757 Msa0121960:CDS 50.0%
! TGTGGTATCGGAGCAGACAT+CGG + chr1_3:48207914-48207933 Msa0121960:CDS 50.0%
TGCTAGAGACGCCATTGCAA+TGG + chr1_3:48208043-48208062 Msa0121960:CDS 50.0%
TGCAAATAGATCAGCCGAGC+TGG + chr1_3:48208118-48208137 Msa0121960:intron 50.0%
GCAAATAGATCAGCCGAGCT+GGG + chr1_3:48208119-48208138 Msa0121960:intron 50.0%
AGCCGGATCTCCAATTTCAC+AGG + chr1_3:48209466-48209485 Msa0121960:intron 50.0%
!! CCAAGGACATGGAGGCATTA+TGG - chr1_3:48209766-48209785 None:intergenic 50.0%
CCATAATGCCTCCATGTCCT+TGG + chr1_3:48209763-48209782 Msa0121960:CDS 50.0%
AGTGAGAACCAAGGACATGG+AGG - chr1_3:48209774-48209793 None:intergenic 50.0%
ACGAGTGAGAACCAAGGACA+TGG - chr1_3:48209777-48209796 None:intergenic 50.0%
! GAAGCCACGGTTGCTCTTTT+AGG + chr1_3:48209821-48209840 Msa0121960:CDS 50.0%
CCACTATGGGGTATAGTCTG+TGG - chr1_3:48209990-48210009 None:intergenic 50.0%
CCACAGACTATACCCCATAG+TGG + chr1_3:48209987-48210006 Msa0121960:CDS 50.0%
! CTAGCGTTGGATCCACTATG+GGG - chr1_3:48210002-48210021 None:intergenic 50.0%
! GTCTAGCGTTGGATCCACTA+TGG - chr1_3:48210004-48210023 None:intergenic 50.0%
GCATAAGGGTCTTCTCACTG+TGG + chr1_3:48210155-48210174 Msa0121960:CDS 50.0%
TTTAGCAACGTGGCGTGGTG+TGG + chr1_3:48207896-48207915 Msa0121960:CDS 55.0%
AACGTGGCGTGGTGTGGTAT+CGG + chr1_3:48207902-48207921 Msa0121960:CDS 55.0%
AGGAGTTGCCCGTCCCTAAA+AGG + chr1_3:48208094-48208113 Msa0121960:intron 55.0%
GGCGGCACTTCCTGTGAAAT+TGG - chr1_3:48209479-48209498 None:intergenic 55.0%
! TTATGCGGAGCGGTTCGGCT+CGG - chr1_3:48208970-48208989 None:intergenic 60.0%
TTCGGCTCTTGGGCAGCTGG+TGG - chr1_3:48207681-48207700 None:intergenic 65.0%
CTCTTCGGCTCTTGGGCAGC+TGG - chr1_3:48207684-48207703 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 48207080 48210594 48207080 ID=Msa0121960;Name=Msa0121960
chr1_3 mRNA 48207080 48210594 48207080 ID=Msa0121960-mRNA-1;Parent=Msa0121960;Name=Msa0121960-mRNA-1;_AED=0.11;_eAED=0.11;_QI=21|1|1|1|0.4|0.33|6|220|324
chr1_3 exon 48207080 48207167 48207080 ID=Msa0121960-mRNA-1:exon:14549;Parent=Msa0121960-mRNA-1
chr1_3 exon 48207648 48207793 48207648 ID=Msa0121960-mRNA-1:exon:14550;Parent=Msa0121960-mRNA-1
chr1_3 exon 48207876 48208064 48207876 ID=Msa0121960-mRNA-1:exon:14551;Parent=Msa0121960-mRNA-1
chr1_3 exon 48209677 48209842 48209677 ID=Msa0121960-mRNA-1:exon:14552;Parent=Msa0121960-mRNA-1
chr1_3 exon 48209947 48210388 48209947 ID=Msa0121960-mRNA-1:exon:14553;Parent=Msa0121960-mRNA-1
chr1_3 exon 48210410 48210594 48210410 ID=Msa0121960-mRNA-1:exon:14554;Parent=Msa0121960-mRNA-1
chr1_3 five_prime_UTR 48207080 48207100 48207080 ID=Msa0121960-mRNA-1:five_prime_utr;Parent=Msa0121960-mRNA-1
chr1_3 CDS 48207101 48207167 48207101 ID=Msa0121960-mRNA-1:cds;Parent=Msa0121960-mRNA-1
chr1_3 CDS 48207648 48207793 48207648 ID=Msa0121960-mRNA-1:cds;Parent=Msa0121960-mRNA-1
chr1_3 CDS 48207876 48208064 48207876 ID=Msa0121960-mRNA-1:cds;Parent=Msa0121960-mRNA-1
chr1_3 CDS 48209677 48209842 48209677 ID=Msa0121960-mRNA-1:cds;Parent=Msa0121960-mRNA-1
chr1_3 CDS 48209947 48210353 48209947 ID=Msa0121960-mRNA-1:cds;Parent=Msa0121960-mRNA-1
chr1_3 three_prime_UTR 48210354 48210388 48210354 ID=Msa0121960-mRNA-1:three_prime_utr;Parent=Msa0121960-mRNA-1
chr1_3 three_prime_UTR 48210410 48210594 48210410 ID=Msa0121960-mRNA-1:three_prime_utr;Parent=Msa0121960-mRNA-1
Gene Sequence

>Msa0121960

ATGGATAACAAGCCTCGTTCTAATTCCTATTTCCTCTTTCTATTGCTTATCCTACATTTTAACTTGGGGAAAAGCCAGCTCCAGCTGAACTACTACTCCACCAGCTGCCCAAGAGCCGAAGAGATAATCAAGCAACAAGTCACTGAACTATATAATCAAGGAAGCACAGCCATTTCGTGGCTTCGAAATCTCTTCCATGATTGCATGGTCAATTCATGCGATGCATCGTTTCTTTTAGCAACGTGGCGTGGTGTGGTATCGGAGCAGACATCGGTAAGAAGTTTTGGGATGAGAAATTTCAAGTACGTGAACACCATCAAAGCTGCTGTTGAGAAAGAATGTCCCTTGACAGTATCATGTGCTGATATTGTCGCTCTTTCTGCTAGAGACGCCATTGCAATGTTAGGAATTCCAAACATTGAAATGAAGAGTGGAAGAAGGGATAGCAAAGAAAGCTATGTAAAGGTGGTGGAAGAATCCATTCCAAACCATAATGCCTCCATGTCCTTGGTTCTCACTCGTTTCCAAGCTATTGGTGTTGATGTTGAAGCCACGGTTGCTCTTTTAGGAGCACATTCAGTGGGAAGAGTACACTGCATAAATATGGTCCACAGACTATACCCCATAGTGGATCCAACGCTAGACCCTACATATGTTTCCTACCTAAAACGTAGATGTCCAAAACCAAATCCAAAGGCAAAAGCTGTCGAATATGTGAGGAATGATCTAAAAACTCCATTGATAATTGATAACAACTACTACAAGAACATTTTGCAGCATAAGGGTCTTCTCACTGTGGATGAAGAACTAGCCACTGACCCAATAACATCACCTTATGTACAAAAAATGGCAGATGATAATGGTTACTTCAATGAGCAATTCTCAAGGGCTGTTCTTTTGTTATCAGAGAATAATCCTCTTATTGGAGATCAAGGTGAAATTAGAAAGGATTGTCGCTTCGTTAATGCCAAATAG
Protein sequence

>Msa0121960

MDNKPRSNSYFLFLLLILHFNLGKSQLQLNYYSTSCPRAEEIIKQQVTELYNQGSTAISWLRNLFHDCMVNSCDASFLLATWRGVVSEQTSVRSFGMRNFKYVNTIKAAVEKECPLTVSCADIVALSARDAIAMLGIPNIEMKSGRRDSKESYVKVVEESIPNHNASMSLVLTRFQAIGVDVEATVALLGAHSVGRVHCINMVHRLYPIVDPTLDPTYVSYLKRRCPKPNPKAKAVEYVRNDLKTPLIIDNNYYKNILQHKGLLTVDEELATDPITSPYVQKMADDNGYFNEQFSRAVLLLSENNPLIGDQGEIRKDCRFVNAK*