AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0123190

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0123190 MtrunA17_Chr1g0183761 97.706 218 2 1 8 225 1 215 2.82e-158 436
Msa0123190 MtrunA17_Chr7g0261381 76.289 194 34 2 1 182 1 194 1.03e-90 265
Msa0123190 MtrunA17_Chr1g0167411 95.200 125 6 0 52 176 65 189 1.98e-89 262
Msa0123190 MtrunA17_Chr1g0187671 80.925 173 28 2 13 182 13 183 4.29e-87 256
Msa0123190 MtrunA17_Chr4g0051251 83.212 137 23 0 51 187 45 181 6.06e-85 251
Msa0123190 MtrunA17_Chr5g0431211 78.322 143 29 1 41 183 28 168 4.67e-83 246
Msa0123190 MtrunA17_Chr3g0105921 81.818 132 24 0 52 183 42 173 1.07e-72 220
Msa0123190 MtrunA17_Chr2g0325831 75.182 137 32 1 39 175 12 146 1.73e-72 218
Msa0123190 MtrunA17_Chr4g0041141 67.105 152 50 0 24 175 1 152 2.44e-72 218
Msa0123190 MtrunA17_Chr7g0274631 66.879 157 48 3 28 183 5 158 1.98e-71 215
Msa0123190 MtrunA17_Chr2g0283861 72.143 140 39 0 43 182 26 165 6.67e-71 214
Msa0123190 MtrunA17_Chr2g0315791 75.000 132 33 0 51 182 32 163 1.10e-69 211
Msa0123190 MtrunA17_Chr1g0190231 83.200 125 21 0 52 176 40 164 1.05e-68 208
Msa0123190 MtrunA17_Chr8g0379061 72.800 125 34 0 51 175 84 208 8.03e-55 175
Msa0123190 MtrunA17_Chr1g0211641 91.111 45 4 0 46 90 65 109 3.86e-24 92.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0123190 AT2G31160.1 75.000 188 39 3 1 183 1 185 1.88e-86 255
Msa0123190 AT1G07090.1 70.625 160 47 0 39 198 18 177 1.61e-83 247
Msa0123190 AT3G23290.2 93.651 126 8 0 51 176 47 172 1.73e-78 234
Msa0123190 AT5G58500.1 79.104 134 27 1 51 183 18 151 4.59e-78 233
Msa0123190 AT2G42610.1 75.524 143 35 0 41 183 14 156 3.43e-73 220
Msa0123190 AT2G42610.2 75.524 143 35 0 41 183 14 156 3.43e-73 220
Msa0123190 AT5G28490.1 83.459 133 22 0 49 181 22 154 5.10e-71 215
Msa0123190 AT3G04510.1 77.778 135 30 0 52 186 33 167 6.98e-69 210
Msa0123190 AT1G78815.1 67.200 125 41 0 51 175 39 163 1.05e-61 191
Msa0123190 AT4G18610.1 69.048 126 38 1 51 175 36 161 5.86e-61 189
Msa0123190 AT1G16910.1 62.121 132 50 0 49 180 20 151 1.32e-57 180

Find 85 sgRNAs with CRISPR-Local

Find 261 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTTTGATGTACTAATAATT+TGG 0.131133 1_3:-50126703 None:intergenic
GCACCAAATGGATTTGTTTC+TGG 0.131233 1_3:-50127784 None:intergenic
TGTTCCAGTCACGGCGTTTC+TGG 0.158859 1_3:-50127042 None:intergenic
ATGCACTGATAGGTCGTTTA+AGG 0.159661 1_3:+50127734 Msa0123190:CDS
TAATGACAACATTTAATTAA+TGG 0.175719 1_3:+50128263 Msa0123190:three_prime_UTR
CTACCCCAAGCTTGTCTTAA+TGG 0.193812 1_3:-50127706 None:intergenic
CGCCGTGACTGGAACACTTT+TGG 0.268565 1_3:+50127049 Msa0123190:CDS
GTTTGATGTACTAATAATTT+GGG 0.281139 1_3:-50126702 None:intergenic
ACACCGATTTGTCCATTCTA+TGG 0.288093 1_3:+50127652 Msa0123190:CDS
CTAGAAAAGGATTCAATCTA+TGG 0.293528 1_3:+50126859 Msa0123190:exon
GTTGGTAGCTCTTCACCTTC+TGG 0.302723 1_3:+50126989 Msa0123190:CDS
TAGTAGTAGCTAGTAGGTTT+AGG 0.306816 1_3:+50128115 Msa0123190:three_prime_UTR
TGTAGGTACTTGGACCAATT+TGG 0.320730 1_3:+50127613 Msa0123190:intron
CCAGGTGCAACTCAATAAAA+TGG 0.335707 1_3:+50128009 Msa0123190:exon
AAAGGAAACAAACTCTGATT+TGG 0.342923 1_3:-50126584 None:intergenic
TCACTCTGGTCACTGTCTTT+AGG 0.344089 1_3:+50126780 Msa0123190:five_prime_UTR
ATAACTAATACCTCTTGCTT+TGG 0.355096 1_3:-50127851 None:intergenic
GGACATGGTGCTGGAGGGTT+TGG 0.355681 1_3:-50127679 None:intergenic
GAGTGAGGGAGTGAGAATAT+AGG 0.363658 1_3:-50126759 None:intergenic
TTGAAGAGATAGATGGTATA+TGG 0.385717 1_3:-50126514 None:intergenic
ATGTCCATTAAGACAAGCTT+GGG 0.387177 1_3:+50127702 Msa0123190:CDS
GTTGCTGCTGTGGGTAGTTA+TGG 0.388764 1_3:-50126945 None:intergenic
AAGTACTAGTAGTAGCTAGT+AGG 0.399769 1_3:+50128109 Msa0123190:three_prime_UTR
CTCATGTTCTTGAATTTCTC+CGG 0.420657 1_3:+50127125 Msa0123190:CDS
TGCACTGATAGGTCGTTTAA+GGG 0.427076 1_3:+50127735 Msa0123190:CDS
AGGTGAAGAGCTACCAACTA+TGG 0.428010 1_3:-50126984 None:intergenic
AGAGAAATTGAAGAGATAGA+TGG 0.429616 1_3:-50126521 None:intergenic
TTAGTTATGAGAAGAAGAAA+AGG 0.431228 1_3:+50127866 Msa0123190:CDS
AAAACCAGAAACGCCGTGAC+TGG 0.451024 1_3:+50127038 Msa0123190:CDS
GACGGTGATTCTTGAGGTAC+TGG 0.454610 1_3:-50127072 None:intergenic
CTAACAGCTCTAGCACCAAA+TGG 0.473078 1_3:-50127796 None:intergenic
TGCTGAAGCTGAAGCTGTTG+TGG 0.480517 1_3:-50127910 None:intergenic
GAGGGAGTGAGAATATAGGA+TGG 0.481699 1_3:-50126755 None:intergenic
AAGCCAGAAACAAATCCATT+TGG 0.482999 1_3:+50127781 Msa0123190:CDS
AGCTGTTGTGGTTGTTGTTG+TGG 0.487063 1_3:-50127898 None:intergenic
TGATGGTGTTGTTGCTGCTG+TGG 0.491697 1_3:-50126955 None:intergenic
TCTGAAAATTAAGCTAGAAA+AGG 0.492970 1_3:+50126846 Msa0123190:five_prime_UTR
GAGATAGATGGTATATGGTA+TGG 0.493261 1_3:-50126509 None:intergenic
TACCTTGAAAGAAAATGAAA+AGG 0.507170 1_3:-50126602 None:intergenic
GGCTATTCTCTCATATTCCA+TGG 0.510761 1_3:+50128030 Msa0123190:three_prime_UTR
TATTGAGTTGCACCTGGAGG+TGG 0.510921 1_3:-50128003 None:intergenic
GACAGTGACCAGAGTGAGTG+AGG 0.514785 1_3:-50126774 None:intergenic
CAACATCAGCTTCCACCTCC+AGG 0.518219 1_3:+50127991 Msa0123190:CDS
GGGTTTGGATGTCCATAGAA+TGG 0.522857 1_3:-50127664 None:intergenic
AATGGACATGGACATGGTGC+TGG 0.525882 1_3:-50127688 None:intergenic
CATGTCCATTAAGACAAGCT+TGG 0.527150 1_3:+50127701 Msa0123190:CDS
TCTTGCTTCACCACTTGAAG+AGG 0.527445 1_3:+50126485 Msa0123190:five_prime_UTR
GTGGGTAGTTATGGTGCTGT+TGG 0.531338 1_3:-50126936 None:intergenic
TGTCCATAGAATGGACAAAT+CGG 0.533345 1_3:-50127655 None:intergenic
AATATTGTGATGTAGGTACT+TGG 0.534027 1_3:+50127603 Msa0123190:intron
GATGGTGTTGTTGCTGCTGT+GGG 0.540954 1_3:-50126954 None:intergenic
GAGGTGGAAGCTGATGTTGA+TGG 0.546837 1_3:-50127987 None:intergenic
CTGCATCTAGAGAGGGAAAG+AGG 0.549386 1_3:-50127097 None:intergenic
GGTAGTCTTGATGCACTGAT+AGG 0.557304 1_3:+50127724 Msa0123190:CDS
GGCCAAAAGTGTTCCAGTCA+CGG 0.557625 1_3:-50127051 None:intergenic
CAAGCTTGTCTTAATGGACA+TGG 0.568355 1_3:-50127700 None:intergenic
ACCATCAGCAACGCCATAGT+TGG 0.569463 1_3:+50126971 Msa0123190:CDS
TTCATAGCGGCTGCTTGTGG+TGG 0.573016 1_3:-50127017 None:intergenic
AGAGGCAATTGCAATTGCAT+TGG 0.573224 1_3:-50127949 None:intergenic
ACCAACTATGGCGTTGCTGA+TGG 0.573256 1_3:-50126972 None:intergenic
TCTCACTCCCTCACTCACTC+TGG 0.573433 1_3:+50126766 Msa0123190:five_prime_UTR
TGTTAGACTTTACCTTCGTG+AGG 0.586964 1_3:+50127813 Msa0123190:CDS
CTAATTGATGTACATGGAGA+AGG 0.590912 1_3:+50128347 Msa0123190:three_prime_UTR
GAGCACCGCTGCATCTAGAG+AGG 0.594373 1_3:-50127105 None:intergenic
GGACATGGACATGGTGCTGG+AGG 0.596653 1_3:-50127685 None:intergenic
AGTTAACTAATTGATGTACA+TGG 0.599715 1_3:+50128341 Msa0123190:three_prime_UTR
GATCTTCAATCCAAAGCAAG+AGG 0.603866 1_3:+50127841 Msa0123190:CDS
TAGTTATGGTGCTGTTGGTG+AGG 0.606112 1_3:-50126931 None:intergenic
GAGGAGGACGGTGATTCTTG+AGG 0.610671 1_3:-50127078 None:intergenic
TTGAAGTAGCAGAAAAGTGG+TGG 0.618514 1_3:+50128306 Msa0123190:three_prime_UTR
TAGTTGAAGTAGCAGAAAAG+TGG 0.618989 1_3:+50128303 Msa0123190:three_prime_UTR
ACAGTGACCAGAGTGAGTGA+GGG 0.621654 1_3:-50126773 None:intergenic
CTTTCCCTCTCTAGATGCAG+CGG 0.626380 1_3:+50127100 Msa0123190:CDS
TGTCTTAATGGACATGGACA+TGG 0.626839 1_3:-50127694 None:intergenic
TGTTGTGGTTGTTGTTGTGG+TGG 0.627258 1_3:-50127895 None:intergenic
TGGTGATGATGAAGATGAAG+AGG 0.634389 1_3:-50127967 None:intergenic
AAGTAGCAGAAAAGTGGTGG+TGG 0.637663 1_3:+50128309 Msa0123190:three_prime_UTR
AGCACCGCTGCATCTAGAGA+GGG 0.651203 1_3:-50127104 None:intergenic
GACATGGACATGGTGCTGGA+GGG 0.653330 1_3:-50127684 None:intergenic
TAGAGAGGGAAAGAGGAGGA+CGG 0.658514 1_3:-50127090 None:intergenic
ATGGTATGGACCTCTTCAAG+TGG 0.664910 1_3:-50126495 None:intergenic
CTTGTGGTGGTAGAGCCAGA+AGG 0.676492 1_3:-50127004 None:intergenic
CATCTAGAGAGGGAAAGAGG+AGG 0.683732 1_3:-50127094 None:intergenic
GAAGATCACGAACCTCACGA+AGG 0.739048 1_3:-50127825 None:intergenic
TGTCCATTAAGACAAGCTTG+GGG 0.750306 1_3:+50127703 Msa0123190:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAATTATAAAAAAAAAT+TGG - chr1_3:50127392-50127411 None:intergenic 0.0%
!! AATAAATTATAAAAAAAAAT+TGG - chr1_3:50127392-50127411 None:intergenic 0.0%
!!! TTTTTTTTATAAACAAGTTT+TGG + chr1_3:50127197-50127216 Msa0123190:intron 10.0%
!!! TTTTTTTTATAAACAAGTTT+TGG + chr1_3:50127197-50127216 Msa0123190:intron 10.0%
!!! AAGATTTTTTATGTTAAATG+AGG - chr1_3:50126466-50126485 None:intergenic 15.0%
!! TTTCTTTCTAACTAATTATT+AGG + chr1_3:50127329-50127348 Msa0123190:intron 15.0%
!! TTCTTTCTAACTAATTATTA+GGG + chr1_3:50127330-50127349 Msa0123190:intron 15.0%
!! TAATGACAACATTTAATTAA+TGG + chr1_3:50128263-50128282 Msa0123190:three_prime_UTR 15.0%
!!! AAGATTTTTTATGTTAAATG+AGG - chr1_3:50126466-50126485 None:intergenic 15.0%
!! TTTCTTTCTAACTAATTATT+AGG + chr1_3:50127329-50127348 Msa0123190:intron 15.0%
!! TTCTTTCTAACTAATTATTA+GGG + chr1_3:50127330-50127349 Msa0123190:intron 15.0%
!! TAATGACAACATTTAATTAA+TGG + chr1_3:50128263-50128282 Msa0123190:three_prime_UTR 15.0%
!! GTTTGATGTACTAATAATTT+GGG - chr1_3:50126705-50126724 None:intergenic 20.0%
!! AGTTTGATGTACTAATAATT+TGG - chr1_3:50126706-50126725 None:intergenic 20.0%
!!! AGAGAAAAAAATTTGTTCTA+GGG - chr1_3:50126808-50126827 None:intergenic 20.0%
!!! AAGAGAAAAAAATTTGTTCT+AGG - chr1_3:50126809-50126828 None:intergenic 20.0%
!!! CTCATTTCATTTTTTTTTGT+TGG - chr1_3:50127157-50127176 None:intergenic 20.0%
!!! TCATTATTTTCTCTTGAATA+TGG - chr1_3:50127540-50127559 None:intergenic 20.0%
!! CAAAAAATTCTTACAAGATT+AGG - chr1_3:50127572-50127591 None:intergenic 20.0%
!!! CTAATCTTGTAAGAATTTTT+TGG + chr1_3:50127570-50127589 Msa0123190:intron 20.0%
!! AATTGTTAATATTGTGATGT+AGG + chr1_3:50127596-50127615 Msa0123190:intron 20.0%
!!! AATAAAGATAGTTTTTTCCA+TGG - chr1_3:50128050-50128069 None:intergenic 20.0%
!!! TTAAATTTTGTGAAGATTTC+TGG + chr1_3:50128155-50128174 Msa0123190:three_prime_UTR 20.0%
!! GTTTGATGTACTAATAATTT+GGG - chr1_3:50126705-50126724 None:intergenic 20.0%
!! AGTTTGATGTACTAATAATT+TGG - chr1_3:50126706-50126725 None:intergenic 20.0%
!!! AGAGAAAAAAATTTGTTCTA+GGG - chr1_3:50126808-50126827 None:intergenic 20.0%
!!! AAGAGAAAAAAATTTGTTCT+AGG - chr1_3:50126809-50126828 None:intergenic 20.0%
!!! CTCATTTCATTTTTTTTTGT+TGG - chr1_3:50127157-50127176 None:intergenic 20.0%
!!! TCATTATTTTCTCTTGAATA+TGG - chr1_3:50127540-50127559 None:intergenic 20.0%
!! CAAAAAATTCTTACAAGATT+AGG - chr1_3:50127572-50127591 None:intergenic 20.0%
!!! CTAATCTTGTAAGAATTTTT+TGG + chr1_3:50127570-50127589 Msa0123190:intron 20.0%
!! AATTGTTAATATTGTGATGT+AGG + chr1_3:50127596-50127615 Msa0123190:intron 20.0%
!!! AATAAAGATAGTTTTTTCCA+TGG - chr1_3:50128050-50128069 None:intergenic 20.0%
!!! TTAAATTTTGTGAAGATTTC+TGG + chr1_3:50128155-50128174 Msa0123190:three_prime_UTR 20.0%
!! TTCCTTTTCATTTTCTTTCA+AGG + chr1_3:50126600-50126619 Msa0123190:five_prime_UTR 25.0%
! TACCTTGAAAGAAAATGAAA+AGG - chr1_3:50126605-50126624 None:intergenic 25.0%
! TCTGAAAATTAAGCTAGAAA+AGG + chr1_3:50126846-50126865 Msa0123190:five_prime_UTR 25.0%
!! GGATTCAATTCAAGATTTTA+TGG + chr1_3:50126880-50126899 Msa0123190:CDS 25.0%
!! TTTTATGGATTCATGTAACT+CGG + chr1_3:50126895-50126914 Msa0123190:CDS 25.0%
!!! TTTTTTTTGTTGGTATGTAC+CGG - chr1_3:50127147-50127166 None:intergenic 25.0%
!!! GTTTTGGTGAATCAATATTT+TGG + chr1_3:50127213-50127232 Msa0123190:intron 25.0%
! TTAGTTATGAGAAGAAGAAA+AGG + chr1_3:50127866-50127885 Msa0123190:CDS 25.0%
! AGTTAACTAATTGATGTACA+TGG + chr1_3:50128341-50128360 Msa0123190:three_prime_UTR 25.0%
!! TTCCTTTTCATTTTCTTTCA+AGG + chr1_3:50126600-50126619 Msa0123190:five_prime_UTR 25.0%
! TACCTTGAAAGAAAATGAAA+AGG - chr1_3:50126605-50126624 None:intergenic 25.0%
! TCTGAAAATTAAGCTAGAAA+AGG + chr1_3:50126846-50126865 Msa0123190:five_prime_UTR 25.0%
!! GGATTCAATTCAAGATTTTA+TGG + chr1_3:50126880-50126899 Msa0123190:CDS 25.0%
!! TTTTATGGATTCATGTAACT+CGG + chr1_3:50126895-50126914 Msa0123190:CDS 25.0%
!!! TTTTTTTTGTTGGTATGTAC+CGG - chr1_3:50127147-50127166 None:intergenic 25.0%
!!! GTTTTGGTGAATCAATATTT+TGG + chr1_3:50127213-50127232 Msa0123190:intron 25.0%
! TTAGTTATGAGAAGAAGAAA+AGG + chr1_3:50127866-50127885 Msa0123190:CDS 25.0%
! AGTTAACTAATTGATGTACA+TGG + chr1_3:50128341-50128360 Msa0123190:three_prime_UTR 25.0%
TTGAAGAGATAGATGGTATA+TGG - chr1_3:50126517-50126536 None:intergenic 30.0%
AGAGAAATTGAAGAGATAGA+TGG - chr1_3:50126524-50126543 None:intergenic 30.0%
AAAGGAAACAAACTCTGATT+TGG - chr1_3:50126587-50126606 None:intergenic 30.0%
! TTCTCTTTTCAAAACAACAC+TGG + chr1_3:50126822-50126841 Msa0123190:five_prime_UTR 30.0%
CTAGAAAAGGATTCAATCTA+TGG + chr1_3:50126859-50126878 Msa0123190:exon 30.0%
!! TTGGTGAATCAATATTTTGG+TGG + chr1_3:50127216-50127235 Msa0123190:intron 30.0%
! ATGTACTTGTTAGAAGTTCA+AGG - chr1_3:50127430-50127449 None:intergenic 30.0%
AAGTACATATTCATTCAACC+TGG + chr1_3:50127442-50127461 Msa0123190:intron 30.0%
! ATCTTTTGTATGTCCCATTT+GGG - chr1_3:50127482-50127501 None:intergenic 30.0%
ACATACAAAAGATCGATCTA+GGG + chr1_3:50127489-50127508 Msa0123190:intron 30.0%
AATATTGTGATGTAGGTACT+TGG + chr1_3:50127603-50127622 Msa0123190:intron 30.0%
ATAACTAATACCTCTTGCTT+TGG - chr1_3:50127854-50127873 None:intergenic 30.0%
TTAAATGAGGATATGTGATG+GGG - chr1_3:50126453-50126472 None:intergenic 30.0%
GTTAAATGAGGATATGTGAT+GGG - chr1_3:50126454-50126473 None:intergenic 30.0%
TGTTAAATGAGGATATGTGA+TGG - chr1_3:50126455-50126474 None:intergenic 30.0%
TTGAAGAGATAGATGGTATA+TGG - chr1_3:50126517-50126536 None:intergenic 30.0%
AGAGAAATTGAAGAGATAGA+TGG - chr1_3:50126524-50126543 None:intergenic 30.0%
AAAGGAAACAAACTCTGATT+TGG - chr1_3:50126587-50126606 None:intergenic 30.0%
! TTCTCTTTTCAAAACAACAC+TGG + chr1_3:50126822-50126841 Msa0123190:five_prime_UTR 30.0%
CTAGAAAAGGATTCAATCTA+TGG + chr1_3:50126859-50126878 Msa0123190:exon 30.0%
!! TTGGTGAATCAATATTTTGG+TGG + chr1_3:50127216-50127235 Msa0123190:intron 30.0%
! ATGTACTTGTTAGAAGTTCA+AGG - chr1_3:50127430-50127449 None:intergenic 30.0%
AAGTACATATTCATTCAACC+TGG + chr1_3:50127442-50127461 Msa0123190:intron 30.0%
! ATCTTTTGTATGTCCCATTT+GGG - chr1_3:50127482-50127501 None:intergenic 30.0%
ACATACAAAAGATCGATCTA+GGG + chr1_3:50127489-50127508 Msa0123190:intron 30.0%
AATATTGTGATGTAGGTACT+TGG + chr1_3:50127603-50127622 Msa0123190:intron 30.0%
ATAACTAATACCTCTTGCTT+TGG - chr1_3:50127854-50127873 None:intergenic 30.0%
GAGATAGATGGTATATGGTA+TGG - chr1_3:50126512-50126531 None:intergenic 35.0%
!! GGTTGATTGATTTTACAGAC+TGG - chr1_3:50126566-50126585 None:intergenic 35.0%
CTCATGTTCTTGAATTTCTC+CGG + chr1_3:50127125-50127144 Msa0123190:CDS 35.0%
! GATCTTTTGTATGTCCCATT+TGG - chr1_3:50127483-50127502 None:intergenic 35.0%
GACATACAAAAGATCGATCT+AGG + chr1_3:50127488-50127507 Msa0123190:intron 35.0%
! GAACTTTTGTCTTGCCAAAT+TGG - chr1_3:50127630-50127649 None:intergenic 35.0%
TGTCCATAGAATGGACAAAT+CGG - chr1_3:50127658-50127677 None:intergenic 35.0%
ATGTCCATTAAGACAAGCTT+GGG + chr1_3:50127702-50127721 Msa0123190:CDS 35.0%
AAGCCAGAAACAAATCCATT+TGG + chr1_3:50127781-50127800 Msa0123190:CDS 35.0%
AAGTACTAGTAGTAGCTAGT+AGG + chr1_3:50128109-50128128 Msa0123190:three_prime_UTR 35.0%
TAGTAGTAGCTAGTAGGTTT+AGG + chr1_3:50128115-50128134 Msa0123190:three_prime_UTR 35.0%
TAGTTGAAGTAGCAGAAAAG+TGG + chr1_3:50128303-50128322 Msa0123190:three_prime_UTR 35.0%
CTAATTGATGTACATGGAGA+AGG + chr1_3:50128347-50128366 Msa0123190:three_prime_UTR 35.0%
GAGATAGATGGTATATGGTA+TGG - chr1_3:50126512-50126531 None:intergenic 35.0%
!! GGTTGATTGATTTTACAGAC+TGG - chr1_3:50126566-50126585 None:intergenic 35.0%
CTCATGTTCTTGAATTTCTC+CGG + chr1_3:50127125-50127144 Msa0123190:CDS 35.0%
! GATCTTTTGTATGTCCCATT+TGG - chr1_3:50127483-50127502 None:intergenic 35.0%
GACATACAAAAGATCGATCT+AGG + chr1_3:50127488-50127507 Msa0123190:intron 35.0%
! GAACTTTTGTCTTGCCAAAT+TGG - chr1_3:50127630-50127649 None:intergenic 35.0%
TGTCCATAGAATGGACAAAT+CGG - chr1_3:50127658-50127677 None:intergenic 35.0%
ATGTCCATTAAGACAAGCTT+GGG + chr1_3:50127702-50127721 Msa0123190:CDS 35.0%
AAGCCAGAAACAAATCCATT+TGG + chr1_3:50127781-50127800 Msa0123190:CDS 35.0%
AAGTACTAGTAGTAGCTAGT+AGG + chr1_3:50128109-50128128 Msa0123190:three_prime_UTR 35.0%
TAGTAGTAGCTAGTAGGTTT+AGG + chr1_3:50128115-50128134 Msa0123190:three_prime_UTR 35.0%
TAGTTGAAGTAGCAGAAAAG+TGG + chr1_3:50128303-50128322 Msa0123190:three_prime_UTR 35.0%
CTAATTGATGTACATGGAGA+AGG + chr1_3:50128347-50128366 Msa0123190:three_prime_UTR 35.0%
AGAGATTGAATCCTGAAACC+TGG + chr1_3:50127258-50127277 Msa0123190:intron 40.0%
CAAAGAACAAGAGATGTTCC+AGG - chr1_3:50127279-50127298 None:intergenic 40.0%
TTCATTCAACCTGGACTCAA+AGG + chr1_3:50127451-50127470 Msa0123190:intron 40.0%
!! ACTCAAAGGATTAGCCCAAA+TGG + chr1_3:50127465-50127484 Msa0123190:intron 40.0%
!! CTCAAAGGATTAGCCCAAAT+GGG + chr1_3:50127466-50127485 Msa0123190:intron 40.0%
TGTAGGTACTTGGACCAATT+TGG + chr1_3:50127613-50127632 Msa0123190:intron 40.0%
!! ACACCGATTTGTCCATTCTA+TGG + chr1_3:50127652-50127671 Msa0123190:CDS 40.0%
! TGTCTTAATGGACATGGACA+TGG - chr1_3:50127697-50127716 None:intergenic 40.0%
! CAAGCTTGTCTTAATGGACA+TGG - chr1_3:50127703-50127722 None:intergenic 40.0%
CATGTCCATTAAGACAAGCT+TGG + chr1_3:50127701-50127720 Msa0123190:CDS 40.0%
TGTCCATTAAGACAAGCTTG+GGG + chr1_3:50127703-50127722 Msa0123190:CDS 40.0%
! ATGCACTGATAGGTCGTTTA+AGG + chr1_3:50127734-50127753 Msa0123190:CDS 40.0%
! TGCACTGATAGGTCGTTTAA+GGG + chr1_3:50127735-50127754 Msa0123190:CDS 40.0%
!! CAGCTTTTGAGGAAAATGGA+GGG + chr1_3:50127758-50127777 Msa0123190:CDS 40.0%
! GCACCAAATGGATTTGTTTC+TGG - chr1_3:50127787-50127806 None:intergenic 40.0%
TGTTAGACTTTACCTTCGTG+AGG + chr1_3:50127813-50127832 Msa0123190:CDS 40.0%
GATCTTCAATCCAAAGCAAG+AGG + chr1_3:50127841-50127860 Msa0123190:CDS 40.0%
AGAGGCAATTGCAATTGCAT+TGG - chr1_3:50127952-50127971 None:intergenic 40.0%
! TGGTGATGATGAAGATGAAG+AGG - chr1_3:50127970-50127989 None:intergenic 40.0%
!! TTTTATTGAGTTGCACCTGG+AGG - chr1_3:50128009-50128028 None:intergenic 40.0%
!! CCATTTTATTGAGTTGCACC+TGG - chr1_3:50128012-50128031 None:intergenic 40.0%
CCAGGTGCAACTCAATAAAA+TGG + chr1_3:50128009-50128028 Msa0123190:exon 40.0%
GGCTATTCTCTCATATTCCA+TGG + chr1_3:50128030-50128049 Msa0123190:three_prime_UTR 40.0%
TTGAAGTAGCAGAAAAGTGG+TGG + chr1_3:50128306-50128325 Msa0123190:three_prime_UTR 40.0%
AGAGATTGAATCCTGAAACC+TGG + chr1_3:50127258-50127277 Msa0123190:intron 40.0%
CAAAGAACAAGAGATGTTCC+AGG - chr1_3:50127279-50127298 None:intergenic 40.0%
TTCATTCAACCTGGACTCAA+AGG + chr1_3:50127451-50127470 Msa0123190:intron 40.0%
!! ACTCAAAGGATTAGCCCAAA+TGG + chr1_3:50127465-50127484 Msa0123190:intron 40.0%
!! CTCAAAGGATTAGCCCAAAT+GGG + chr1_3:50127466-50127485 Msa0123190:intron 40.0%
TGTAGGTACTTGGACCAATT+TGG + chr1_3:50127613-50127632 Msa0123190:intron 40.0%
!! ACACCGATTTGTCCATTCTA+TGG + chr1_3:50127652-50127671 Msa0123190:CDS 40.0%
! TGTCTTAATGGACATGGACA+TGG - chr1_3:50127697-50127716 None:intergenic 40.0%
! CAAGCTTGTCTTAATGGACA+TGG - chr1_3:50127703-50127722 None:intergenic 40.0%
CATGTCCATTAAGACAAGCT+TGG + chr1_3:50127701-50127720 Msa0123190:CDS 40.0%
TGTCCATTAAGACAAGCTTG+GGG + chr1_3:50127703-50127722 Msa0123190:CDS 40.0%
! ATGCACTGATAGGTCGTTTA+AGG + chr1_3:50127734-50127753 Msa0123190:CDS 40.0%
! TGCACTGATAGGTCGTTTAA+GGG + chr1_3:50127735-50127754 Msa0123190:CDS 40.0%
!! CAGCTTTTGAGGAAAATGGA+GGG + chr1_3:50127758-50127777 Msa0123190:CDS 40.0%
! GCACCAAATGGATTTGTTTC+TGG - chr1_3:50127787-50127806 None:intergenic 40.0%
TGTTAGACTTTACCTTCGTG+AGG + chr1_3:50127813-50127832 Msa0123190:CDS 40.0%
GATCTTCAATCCAAAGCAAG+AGG + chr1_3:50127841-50127860 Msa0123190:CDS 40.0%
AGAGGCAATTGCAATTGCAT+TGG - chr1_3:50127952-50127971 None:intergenic 40.0%
! TGGTGATGATGAAGATGAAG+AGG - chr1_3:50127970-50127989 None:intergenic 40.0%
!! TTTTATTGAGTTGCACCTGG+AGG - chr1_3:50128009-50128028 None:intergenic 40.0%
!! CCATTTTATTGAGTTGCACC+TGG - chr1_3:50128012-50128031 None:intergenic 40.0%
CCAGGTGCAACTCAATAAAA+TGG + chr1_3:50128009-50128028 Msa0123190:exon 40.0%
GGCTATTCTCTCATATTCCA+TGG + chr1_3:50128030-50128049 Msa0123190:three_prime_UTR 40.0%
TTGAAGTAGCAGAAAAGTGG+TGG + chr1_3:50128306-50128325 Msa0123190:three_prime_UTR 40.0%
TCTTGCTTCACCACTTGAAG+AGG + chr1_3:50126485-50126504 Msa0123190:five_prime_UTR 45.0%
ATGGTATGGACCTCTTCAAG+TGG - chr1_3:50126498-50126517 None:intergenic 45.0%
GAGGGAGTGAGAATATAGGA+TGG - chr1_3:50126758-50126777 None:intergenic 45.0%
GAGTGAGGGAGTGAGAATAT+AGG - chr1_3:50126762-50126781 None:intergenic 45.0%
TCACTCTGGTCACTGTCTTT+AGG + chr1_3:50126780-50126799 Msa0123190:five_prime_UTR 45.0%
!! TAGTTATGGTGCTGTTGGTG+AGG - chr1_3:50126934-50126953 None:intergenic 45.0%
AGGTGAAGAGCTACCAACTA+TGG - chr1_3:50126987-50127006 None:intergenic 45.0%
!!! GGCGTTTCTGGTTTTCATAG+CGG - chr1_3:50127033-50127052 None:intergenic 45.0%
CAAGAGATGTTCCAGGTTTC+AGG - chr1_3:50127272-50127291 None:intergenic 45.0%
GGGTTTGGATGTCCATAGAA+TGG - chr1_3:50127667-50127686 None:intergenic 45.0%
CTACCCCAAGCTTGTCTTAA+TGG - chr1_3:50127709-50127728 None:intergenic 45.0%
! GGTAGTCTTGATGCACTGAT+AGG + chr1_3:50127724-50127743 Msa0123190:CDS 45.0%
! TCGTTTAAGGGCAGCTTTTG+AGG + chr1_3:50127747-50127766 Msa0123190:CDS 45.0%
!! AGGGCAGCTTTTGAGGAAAA+TGG + chr1_3:50127754-50127773 Msa0123190:CDS 45.0%
!! GCAGCTTTTGAGGAAAATGG+AGG + chr1_3:50127757-50127776 Msa0123190:CDS 45.0%
! CTAACAGCTCTAGCACCAAA+TGG - chr1_3:50127799-50127818 None:intergenic 45.0%
! TGTTGTGGTTGTTGTTGTGG+TGG - chr1_3:50127898-50127917 None:intergenic 45.0%
! AGCTGTTGTGGTTGTTGTTG+TGG - chr1_3:50127901-50127920 None:intergenic 45.0%
AAGTAGCAGAAAAGTGGTGG+TGG + chr1_3:50128309-50128328 Msa0123190:three_prime_UTR 45.0%
TCTTGCTTCACCACTTGAAG+AGG + chr1_3:50126485-50126504 Msa0123190:five_prime_UTR 45.0%
ATGGTATGGACCTCTTCAAG+TGG - chr1_3:50126498-50126517 None:intergenic 45.0%
GAGGGAGTGAGAATATAGGA+TGG - chr1_3:50126758-50126777 None:intergenic 45.0%
GAGTGAGGGAGTGAGAATAT+AGG - chr1_3:50126762-50126781 None:intergenic 45.0%
TCACTCTGGTCACTGTCTTT+AGG + chr1_3:50126780-50126799 Msa0123190:five_prime_UTR 45.0%
!! TAGTTATGGTGCTGTTGGTG+AGG - chr1_3:50126934-50126953 None:intergenic 45.0%
AGGTGAAGAGCTACCAACTA+TGG - chr1_3:50126987-50127006 None:intergenic 45.0%
!!! GGCGTTTCTGGTTTTCATAG+CGG - chr1_3:50127033-50127052 None:intergenic 45.0%
CAAGAGATGTTCCAGGTTTC+AGG - chr1_3:50127272-50127291 None:intergenic 45.0%
GGGTTTGGATGTCCATAGAA+TGG - chr1_3:50127667-50127686 None:intergenic 45.0%
CTACCCCAAGCTTGTCTTAA+TGG - chr1_3:50127709-50127728 None:intergenic 45.0%
! GGTAGTCTTGATGCACTGAT+AGG + chr1_3:50127724-50127743 Msa0123190:CDS 45.0%
! TCGTTTAAGGGCAGCTTTTG+AGG + chr1_3:50127747-50127766 Msa0123190:CDS 45.0%
!! AGGGCAGCTTTTGAGGAAAA+TGG + chr1_3:50127754-50127773 Msa0123190:CDS 45.0%
!! GCAGCTTTTGAGGAAAATGG+AGG + chr1_3:50127757-50127776 Msa0123190:CDS 45.0%
! CTAACAGCTCTAGCACCAAA+TGG - chr1_3:50127799-50127818 None:intergenic 45.0%
! TGTTGTGGTTGTTGTTGTGG+TGG - chr1_3:50127898-50127917 None:intergenic 45.0%
! AGCTGTTGTGGTTGTTGTTG+TGG - chr1_3:50127901-50127920 None:intergenic 45.0%
AAGTAGCAGAAAAGTGGTGG+TGG + chr1_3:50128309-50128328 Msa0123190:three_prime_UTR 45.0%
ACAGTGACCAGAGTGAGTGA+GGG - chr1_3:50126776-50126795 None:intergenic 50.0%
!! GTGGGTAGTTATGGTGCTGT+TGG - chr1_3:50126939-50126958 None:intergenic 50.0%
GTTGCTGCTGTGGGTAGTTA+TGG - chr1_3:50126948-50126967 None:intergenic 50.0%
!! GATGGTGTTGTTGCTGCTGT+GGG - chr1_3:50126957-50126976 None:intergenic 50.0%
!! TGATGGTGTTGTTGCTGCTG+TGG - chr1_3:50126958-50126977 None:intergenic 50.0%
ACCATCAGCAACGCCATAGT+TGG + chr1_3:50126971-50126990 Msa0123190:CDS 50.0%
ACCAACTATGGCGTTGCTGA+TGG - chr1_3:50126975-50126994 None:intergenic 50.0%
!! GTTGGTAGCTCTTCACCTTC+TGG + chr1_3:50126989-50127008 Msa0123190:CDS 50.0%
! GTTTTCATAGCGGCTGCTTG+TGG - chr1_3:50127023-50127042 None:intergenic 50.0%
AAAACCAGAAACGCCGTGAC+TGG + chr1_3:50127038-50127057 Msa0123190:CDS 50.0%
!! GGCCAAAAGTGTTCCAGTCA+CGG - chr1_3:50127054-50127073 None:intergenic 50.0%
GACGGTGATTCTTGAGGTAC+TGG - chr1_3:50127075-50127094 None:intergenic 50.0%
TAGAGAGGGAAAGAGGAGGA+CGG - chr1_3:50127093-50127112 None:intergenic 50.0%
CATCTAGAGAGGGAAAGAGG+AGG - chr1_3:50127097-50127116 None:intergenic 50.0%
CTGCATCTAGAGAGGGAAAG+AGG - chr1_3:50127100-50127119 None:intergenic 50.0%
CTTTCCCTCTCTAGATGCAG+CGG + chr1_3:50127100-50127119 Msa0123190:CDS 50.0%
TGGGCTAATCCTTTGAGTCC+AGG - chr1_3:50127463-50127482 None:intergenic 50.0%
AATGGACATGGACATGGTGC+TGG - chr1_3:50127691-50127710 None:intergenic 50.0%
GAAGATCACGAACCTCACGA+AGG - chr1_3:50127828-50127847 None:intergenic 50.0%
TGCTGAAGCTGAAGCTGTTG+TGG - chr1_3:50127913-50127932 None:intergenic 50.0%
GAGGTGGAAGCTGATGTTGA+TGG - chr1_3:50127990-50128009 None:intergenic 50.0%
! TATTGAGTTGCACCTGGAGG+TGG - chr1_3:50128006-50128025 None:intergenic 50.0%
ACAGTGACCAGAGTGAGTGA+GGG - chr1_3:50126776-50126795 None:intergenic 50.0%
!! GTGGGTAGTTATGGTGCTGT+TGG - chr1_3:50126939-50126958 None:intergenic 50.0%
GTTGCTGCTGTGGGTAGTTA+TGG - chr1_3:50126948-50126967 None:intergenic 50.0%
!! GATGGTGTTGTTGCTGCTGT+GGG - chr1_3:50126957-50126976 None:intergenic 50.0%
!! TGATGGTGTTGTTGCTGCTG+TGG - chr1_3:50126958-50126977 None:intergenic 50.0%
ACCATCAGCAACGCCATAGT+TGG + chr1_3:50126971-50126990 Msa0123190:CDS 50.0%
ACCAACTATGGCGTTGCTGA+TGG - chr1_3:50126975-50126994 None:intergenic 50.0%
!! GTTGGTAGCTCTTCACCTTC+TGG + chr1_3:50126989-50127008 Msa0123190:CDS 50.0%
! GTTTTCATAGCGGCTGCTTG+TGG - chr1_3:50127023-50127042 None:intergenic 50.0%
AAAACCAGAAACGCCGTGAC+TGG + chr1_3:50127038-50127057 Msa0123190:CDS 50.0%
!! GGCCAAAAGTGTTCCAGTCA+CGG - chr1_3:50127054-50127073 None:intergenic 50.0%
GACGGTGATTCTTGAGGTAC+TGG - chr1_3:50127075-50127094 None:intergenic 50.0%
TAGAGAGGGAAAGAGGAGGA+CGG - chr1_3:50127093-50127112 None:intergenic 50.0%
CATCTAGAGAGGGAAAGAGG+AGG - chr1_3:50127097-50127116 None:intergenic 50.0%
CTGCATCTAGAGAGGGAAAG+AGG - chr1_3:50127100-50127119 None:intergenic 50.0%
CTTTCCCTCTCTAGATGCAG+CGG + chr1_3:50127100-50127119 Msa0123190:CDS 50.0%
TGGGCTAATCCTTTGAGTCC+AGG - chr1_3:50127463-50127482 None:intergenic 50.0%
AATGGACATGGACATGGTGC+TGG - chr1_3:50127691-50127710 None:intergenic 50.0%
GAAGATCACGAACCTCACGA+AGG - chr1_3:50127828-50127847 None:intergenic 50.0%
TGCTGAAGCTGAAGCTGTTG+TGG - chr1_3:50127913-50127932 None:intergenic 50.0%
GAGGTGGAAGCTGATGTTGA+TGG - chr1_3:50127990-50128009 None:intergenic 50.0%
! TATTGAGTTGCACCTGGAGG+TGG - chr1_3:50128006-50128025 None:intergenic 50.0%
TCTCACTCCCTCACTCACTC+TGG + chr1_3:50126766-50126785 Msa0123190:five_prime_UTR 55.0%
GACAGTGACCAGAGTGAGTG+AGG - chr1_3:50126777-50126796 None:intergenic 55.0%
CTTGTGGTGGTAGAGCCAGA+AGG - chr1_3:50127007-50127026 None:intergenic 55.0%
TTCATAGCGGCTGCTTGTGG+TGG - chr1_3:50127020-50127039 None:intergenic 55.0%
TGTTCCAGTCACGGCGTTTC+TGG - chr1_3:50127045-50127064 None:intergenic 55.0%
! CGCCGTGACTGGAACACTTT+TGG + chr1_3:50127049-50127068 Msa0123190:CDS 55.0%
GAGGAGGACGGTGATTCTTG+AGG - chr1_3:50127081-50127100 None:intergenic 55.0%
AGCACCGCTGCATCTAGAGA+GGG - chr1_3:50127107-50127126 None:intergenic 55.0%
! GACATGGACATGGTGCTGGA+GGG - chr1_3:50127687-50127706 None:intergenic 55.0%
CAACATCAGCTTCCACCTCC+AGG + chr1_3:50127991-50128010 Msa0123190:CDS 55.0%
TCTCACTCCCTCACTCACTC+TGG + chr1_3:50126766-50126785 Msa0123190:five_prime_UTR 55.0%
GACAGTGACCAGAGTGAGTG+AGG - chr1_3:50126777-50126796 None:intergenic 55.0%
CTTGTGGTGGTAGAGCCAGA+AGG - chr1_3:50127007-50127026 None:intergenic 55.0%
TTCATAGCGGCTGCTTGTGG+TGG - chr1_3:50127020-50127039 None:intergenic 55.0%
TGTTCCAGTCACGGCGTTTC+TGG - chr1_3:50127045-50127064 None:intergenic 55.0%
! CGCCGTGACTGGAACACTTT+TGG + chr1_3:50127049-50127068 Msa0123190:CDS 55.0%
GAGGAGGACGGTGATTCTTG+AGG - chr1_3:50127081-50127100 None:intergenic 55.0%
AGCACCGCTGCATCTAGAGA+GGG - chr1_3:50127107-50127126 None:intergenic 55.0%
! GACATGGACATGGTGCTGGA+GGG - chr1_3:50127687-50127706 None:intergenic 55.0%
CAACATCAGCTTCCACCTCC+AGG + chr1_3:50127991-50128010 Msa0123190:CDS 55.0%
! GAGCACCGCTGCATCTAGAG+AGG - chr1_3:50127108-50127127 None:intergenic 60.0%
! GGACATGGTGCTGGAGGGTT+TGG - chr1_3:50127682-50127701 None:intergenic 60.0%
! GGACATGGACATGGTGCTGG+AGG - chr1_3:50127688-50127707 None:intergenic 60.0%
! GAGCACCGCTGCATCTAGAG+AGG - chr1_3:50127108-50127127 None:intergenic 60.0%
! GGACATGGTGCTGGAGGGTT+TGG - chr1_3:50127682-50127701 None:intergenic 60.0%
! GGACATGGACATGGTGCTGG+AGG - chr1_3:50127688-50127707 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 50126453 50128381 50126453 ID=Msa0123190;Name=Msa0123190
chr1_3 mRNA 50126453 50128381 50126453 ID=Msa0123190-mRNA-1;Parent=Msa0123190;Name=Msa0123190-mRNA-1;_AED=0.03;_eAED=0.03;_QI=425|1|1|1|1|1|2|355|225
chr1_3 exon 50126453 50127146 50126453 ID=Msa0123190-mRNA-1:exon:15293;Parent=Msa0123190-mRNA-1
chr1_3 exon 50127618 50128381 50127618 ID=Msa0123190-mRNA-1:exon:15294;Parent=Msa0123190-mRNA-1
chr1_3 five_prime_UTR 50126453 50126877 50126453 ID=Msa0123190-mRNA-1:five_prime_utr;Parent=Msa0123190-mRNA-1
chr1_3 CDS 50126878 50127146 50126878 ID=Msa0123190-mRNA-1:cds;Parent=Msa0123190-mRNA-1
chr1_3 CDS 50127618 50128026 50127618 ID=Msa0123190-mRNA-1:cds;Parent=Msa0123190-mRNA-1
chr1_3 three_prime_UTR 50128027 50128381 50128027 ID=Msa0123190-mRNA-1:three_prime_utr;Parent=Msa0123190-mRNA-1
Gene Sequence

>Msa0123190

ATGGATTCAATTCAAGATTTTATGGATTCATGTAACTCGGACAATAGTTGCAGCCTCACCAACAGCACCATAACTACCCACAGCAGCAACAACACCATCAGCAACGCCATAGTTGGTAGCTCTTCACCTTCTGGCTCTACCACCACAAGCAGCCGCTATGAAAACCAGAAACGCCGTGACTGGAACACTTTTGGCCAGTACCTCAAGAATCACCGTCCTCCTCTTTCCCTCTCTAGATGCAGCGGTGCTCATGTTCTTGAATTTCTCCGGTACTTGGACCAATTTGGCAAGACAAAAGTTCACACACCGATTTGTCCATTCTATGGACATCCAAACCCTCCAGCACCATGTCCATGTCCATTAAGACAAGCTTGGGGTAGTCTTGATGCACTGATAGGTCGTTTAAGGGCAGCTTTTGAGGAAAATGGAGGGAAGCCAGAAACAAATCCATTTGGTGCTAGAGCTGTTAGACTTTACCTTCGTGAGGTTCGTGATCTTCAATCCAAAGCAAGAGGTATTAGTTATGAGAAGAAGAAAAGGAAGCGTCCACCACAACAACAACCACAACAGCTTCAGCTTCAGCAGCAACAACAGCAACAACCAATGCAATTGCAATTGCCTCTTCATCTTCATCATCACCATCAACATCAGCTTCCACCTCCAGGTGCAACTCAATAA
Protein sequence

>Msa0123190

MDSIQDFMDSCNSDNSCSLTNSTITTHSSNNTISNAIVGSSSPSGSTTTSSRYENQKRRDWNTFGQYLKNHRPPLSLSRCSGAHVLEFLRYLDQFGKTKVHTPICPFYGHPNPPAPCPCPLRQAWGSLDALIGRLRAAFEENGGKPETNPFGARAVRLYLREVRDLQSKARGISYEKKKRKRPPQQQPQQLQLQQQQQQQPMQLQLPLHLHHHHQHQLPPPGATQ*