AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0660480

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0660480 MtrunA17_Chr1g0183761 97.706 218 2 1 8 225 1 215 2.82e-158 436
Msa0660480 MtrunA17_Chr7g0261381 76.289 194 34 2 1 182 1 194 1.03e-90 265
Msa0660480 MtrunA17_Chr1g0167411 95.200 125 6 0 52 176 65 189 1.98e-89 262
Msa0660480 MtrunA17_Chr1g0187671 80.925 173 28 2 13 182 13 183 4.29e-87 256
Msa0660480 MtrunA17_Chr4g0051251 83.212 137 23 0 51 187 45 181 6.06e-85 251
Msa0660480 MtrunA17_Chr5g0431211 78.322 143 29 1 41 183 28 168 4.67e-83 246
Msa0660480 MtrunA17_Chr3g0105921 81.818 132 24 0 52 183 42 173 1.07e-72 220
Msa0660480 MtrunA17_Chr2g0325831 75.182 137 32 1 39 175 12 146 1.73e-72 218
Msa0660480 MtrunA17_Chr4g0041141 67.105 152 50 0 24 175 1 152 2.44e-72 218
Msa0660480 MtrunA17_Chr7g0274631 66.879 157 48 3 28 183 5 158 1.98e-71 215
Msa0660480 MtrunA17_Chr2g0283861 72.143 140 39 0 43 182 26 165 6.67e-71 214
Msa0660480 MtrunA17_Chr2g0315791 75.000 132 33 0 51 182 32 163 1.10e-69 211
Msa0660480 MtrunA17_Chr1g0190231 83.200 125 21 0 52 176 40 164 1.05e-68 208
Msa0660480 MtrunA17_Chr8g0379061 72.800 125 34 0 51 175 84 208 8.03e-55 175
Msa0660480 MtrunA17_Chr1g0211641 91.111 45 4 0 46 90 65 109 3.86e-24 92.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0660480 AT2G31160.1 75.000 188 39 3 1 183 1 185 1.88e-86 255
Msa0660480 AT1G07090.1 70.625 160 47 0 39 198 18 177 1.61e-83 247
Msa0660480 AT3G23290.2 93.651 126 8 0 51 176 47 172 1.73e-78 234
Msa0660480 AT5G58500.1 79.104 134 27 1 51 183 18 151 4.59e-78 233
Msa0660480 AT2G42610.1 75.524 143 35 0 41 183 14 156 3.43e-73 220
Msa0660480 AT2G42610.2 75.524 143 35 0 41 183 14 156 3.43e-73 220
Msa0660480 AT5G28490.1 83.459 133 22 0 49 181 22 154 5.10e-71 215
Msa0660480 AT3G04510.1 77.778 135 30 0 52 186 33 167 6.98e-69 210
Msa0660480 AT1G78815.1 67.200 125 41 0 51 175 39 163 1.05e-61 191
Msa0660480 AT4G18610.1 69.048 126 38 1 51 175 36 161 5.86e-61 189
Msa0660480 AT1G16910.1 62.121 132 50 0 49 180 20 151 1.32e-57 180

Find 85 sgRNAs with CRISPR-Local

Find 253 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTTTGATGTACTAATAATT+TGG 0.131133 4_3:-71318994 None:intergenic
GCACCAAATGGATTTGTTTC+TGG 0.131233 4_3:-71320065 None:intergenic
TGTTCCAGTCACGGCGTTTC+TGG 0.158859 4_3:-71319333 None:intergenic
ATGCACTGATAGGTCGTTTA+AGG 0.159661 4_3:+71320015 Msa0660480:CDS
TAATGACAACATTTAATTAA+TGG 0.175719 4_3:+71320541 Msa0660480:three_prime_UTR
CTACCCCAAGCTTGTCTTAA+TGG 0.193812 4_3:-71319987 None:intergenic
CGCCGTGACTGGAACACTTT+TGG 0.268565 4_3:+71319340 Msa0660480:CDS
GTTTGATGTACTAATAATTT+GGG 0.281139 4_3:-71318993 None:intergenic
ACACCGATTTGTCCATTCTA+TGG 0.288093 4_3:+71319933 Msa0660480:CDS
CTAGAAAAGGATTCAATCTA+TGG 0.293528 4_3:+71319150 Msa0660480:exon
GTTGGTAGCTCTTCACCTTC+TGG 0.302723 4_3:+71319280 Msa0660480:CDS
TAGTAGTAGCTAGTAGGTTT+AGG 0.306816 4_3:+71320394 Msa0660480:three_prime_UTR
TGTAGGTACTTGGACCAATT+TGG 0.320730 4_3:+71319894 Msa0660480:intron
CCAGGTGCAACTCAATAAAA+TGG 0.335707 4_3:+71320290 Msa0660480:exon
AAAGGAAACAAACTCTGATT+TGG 0.342923 4_3:-71318868 None:intergenic
ATAACTAATACCTCTTGCTT+TGG 0.355096 4_3:-71320132 None:intergenic
GGACATGGTGCTGGAGGGTT+TGG 0.355681 4_3:-71319960 None:intergenic
TCACTCTGGTCACTGTCTTT+AGG 0.368979 4_3:+71319071 Msa0660480:five_prime_UTR
GAGCGAGGGAGTGAGAATAT+AGG 0.380171 4_3:-71319050 None:intergenic
TTGAAGAGATAGATGGTATA+TGG 0.385717 4_3:-71318798 None:intergenic
ATGTCCATTAAGACAAGCTT+GGG 0.387177 4_3:+71319983 Msa0660480:CDS
GTTGCTGCTGTGGGTAGTTA+TGG 0.388764 4_3:-71319236 None:intergenic
AAGTACTAGTAGTAGCTAGT+AGG 0.399769 4_3:+71320388 Msa0660480:three_prime_UTR
CTCATGTTCTTGAATTTCTC+CGG 0.420657 4_3:+71319416 Msa0660480:CDS
TGCACTGATAGGTCGTTTAA+GGG 0.427076 4_3:+71320016 Msa0660480:CDS
AGGTGAAGAGCTACCAACTA+TGG 0.428010 4_3:-71319275 None:intergenic
AGAGAAATTGAAGAGATAGA+TGG 0.429616 4_3:-71318805 None:intergenic
TTAGTTATGAGAAGAAGAAA+AGG 0.431228 4_3:+71320147 Msa0660480:CDS
AAAACCAGAAACGCCGTGAC+TGG 0.451024 4_3:+71319329 Msa0660480:CDS
GACGGTGATTCTTGAGGTAC+TGG 0.454610 4_3:-71319363 None:intergenic
CTAACAGCTCTAGCACCAAA+TGG 0.473078 4_3:-71320077 None:intergenic
TGCTGAAGCTGAAGCTGTTG+TGG 0.480517 4_3:-71320191 None:intergenic
AAGCCAGAAACAAATCCATT+TGG 0.482999 4_3:+71320062 Msa0660480:CDS
AGCTGTTGTGGTTGTTGTTG+TGG 0.487063 4_3:-71320179 None:intergenic
TGATGGTGTTGTTGCTGCTG+TGG 0.491697 4_3:-71319246 None:intergenic
TCTGAAAATTAAGCTAGAAA+AGG 0.492970 4_3:+71319137 Msa0660480:five_prime_UTR
GAGATAGATGGTATATGGTA+TGG 0.493261 4_3:-71318793 None:intergenic
TATTGAGTTGCACCTGGAGG+TGG 0.510921 4_3:-71320284 None:intergenic
CAACATCAGCTTCCACCTCC+AGG 0.518219 4_3:+71320272 Msa0660480:CDS
TAGCTTGAAAGAAAATGAAA+AGG 0.519967 4_3:-71318886 None:intergenic
GACAGTGACCAGAGTGAGCG+AGG 0.520040 4_3:-71319065 None:intergenic
GGGTTTGGATGTCCATAGAA+TGG 0.522857 4_3:-71319945 None:intergenic
AATGGACATGGACATGGTGC+TGG 0.525882 4_3:-71319969 None:intergenic
CATGTCCATTAAGACAAGCT+TGG 0.527150 4_3:+71319982 Msa0660480:CDS
TCTTGCTTCACCACTTGAAG+AGG 0.527445 4_3:+71318769 Msa0660480:five_prime_UTR
GTGGGTAGTTATGGTGCTGT+TGG 0.531338 4_3:-71319227 None:intergenic
TGTCCATAGAATGGACAAAT+CGG 0.533345 4_3:-71319936 None:intergenic
AATATTGTGATGTAGGTACT+TGG 0.534027 4_3:+71319884 Msa0660480:intron
ATGGCTATTCTCATATTCCA+TGG 0.536637 4_3:+71320309 Msa0660480:three_prime_UTR
GATGGTGTTGTTGCTGCTGT+GGG 0.540954 4_3:-71319245 None:intergenic
GAGGTGGAAGCTGATGTTGA+TGG 0.546837 4_3:-71320268 None:intergenic
CTGCATCTAGAGAGGGAAAG+AGG 0.549386 4_3:-71319388 None:intergenic
GGTAGTCTTGATGCACTGAT+AGG 0.557304 4_3:+71320005 Msa0660480:CDS
GGCCAAAAGTGTTCCAGTCA+CGG 0.557625 4_3:-71319342 None:intergenic
CAAGCTTGTCTTAATGGACA+TGG 0.568355 4_3:-71319981 None:intergenic
ACCATCAGCAACGCCATAGT+TGG 0.569463 4_3:+71319262 Msa0660480:CDS
TCTCACTCCCTCGCTCACTC+TGG 0.571806 4_3:+71319057 Msa0660480:five_prime_UTR
GAGGGAGTGAGAATATAGGA+TGG 0.572011 4_3:-71319046 None:intergenic
TTCATAGCGGCTGCTTGTGG+TGG 0.573016 4_3:-71319308 None:intergenic
AGAGGCAATTGCAATTGCAT+TGG 0.573224 4_3:-71320230 None:intergenic
ACCAACTATGGCGTTGCTGA+TGG 0.573256 4_3:-71319263 None:intergenic
TGTTAGACTTTACCTTCGTG+AGG 0.586964 4_3:+71320094 Msa0660480:CDS
CTAATTGATGTACATGGAGA+AGG 0.590912 4_3:+71320625 Msa0660480:three_prime_UTR
GAGCACCGCTGCATCTAGAG+AGG 0.594373 4_3:-71319396 None:intergenic
GGACATGGACATGGTGCTGG+AGG 0.596653 4_3:-71319966 None:intergenic
AGTTAACTAATTGATGTACA+TGG 0.599715 4_3:+71320619 Msa0660480:three_prime_UTR
GATCTTCAATCCAAAGCAAG+AGG 0.603866 4_3:+71320122 Msa0660480:CDS
TAGTTATGGTGCTGTTGGTG+AGG 0.606112 4_3:-71319222 None:intergenic
GAGGAGGACGGTGATTCTTG+AGG 0.610671 4_3:-71319369 None:intergenic
TTGAAGTAGCAGAAAAGTGG+TGG 0.618514 4_3:+71320584 Msa0660480:three_prime_UTR
TAGTTGAAGTAGCAGAAAAG+TGG 0.618989 4_3:+71320581 Msa0660480:three_prime_UTR
CTTTCCCTCTCTAGATGCAG+CGG 0.626380 4_3:+71319391 Msa0660480:CDS
TGTCTTAATGGACATGGACA+TGG 0.626839 4_3:-71319975 None:intergenic
TGTTGTGGTTGTTGTTGTGG+TGG 0.627258 4_3:-71320176 None:intergenic
ACAGTGACCAGAGTGAGCGA+GGG 0.631192 4_3:-71319064 None:intergenic
TGGTGATGATGAAGATGAAG+AGG 0.634389 4_3:-71320248 None:intergenic
AAGTAGCAGAAAAGTGGTGG+TGG 0.637663 4_3:+71320587 Msa0660480:three_prime_UTR
AGCACCGCTGCATCTAGAGA+GGG 0.651203 4_3:-71319395 None:intergenic
GACATGGACATGGTGCTGGA+GGG 0.653330 4_3:-71319965 None:intergenic
TAGAGAGGGAAAGAGGAGGA+CGG 0.658514 4_3:-71319381 None:intergenic
ATGGTATGGACCTCTTCAAG+TGG 0.664910 4_3:-71318779 None:intergenic
CTTGTGGTGGTAGAGCCAGA+AGG 0.676492 4_3:-71319295 None:intergenic
CATCTAGAGAGGGAAAGAGG+AGG 0.683732 4_3:-71319385 None:intergenic
GAAGATCACGAACCTCACGA+AGG 0.739048 4_3:-71320106 None:intergenic
TGTCCATTAAGACAAGCTTG+GGG 0.750306 4_3:+71319984 Msa0660480:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTTTATAAACAAGTTT+TGG + chr4_3:71319487-71319506 Msa0660480:intron 10.0%
!! AAAAAATATATCGAAAAAAT+TGG - chr4_3:71319693-71319712 None:intergenic 10.0%
!!! ATTTTTTTATAAACAAGTTT+TGG + chr4_3:71319487-71319506 Msa0660480:intron 10.0%
!! AAAAAATATATCGAAAAAAT+TGG - chr4_3:71319693-71319712 None:intergenic 10.0%
!!! AAGATTTTTTATGTTAAATG+AGG - chr4_3:71318750-71318769 None:intergenic 15.0%
!! TAATGACAACATTTAATTAA+TGG + chr4_3:71320541-71320560 Msa0660480:three_prime_UTR 15.0%
!!! AAGATTTTTTATGTTAAATG+AGG - chr4_3:71318750-71318769 None:intergenic 15.0%
!! TAATGACAACATTTAATTAA+TGG + chr4_3:71320541-71320560 Msa0660480:three_prime_UTR 15.0%
!! GTTTGATGTACTAATAATTT+GGG - chr4_3:71318996-71319015 None:intergenic 20.0%
!! AGTTTGATGTACTAATAATT+TGG - chr4_3:71318997-71319016 None:intergenic 20.0%
!!! AGAGAAAAAAATTTGTTCTA+GGG - chr4_3:71319099-71319118 None:intergenic 20.0%
!!! AAGAGAAAAAAATTTGTTCT+AGG - chr4_3:71319100-71319119 None:intergenic 20.0%
!!! TTTTTCCCTAACTAATTATT+AGG + chr4_3:71319617-71319636 Msa0660480:intron 20.0%
!!! TTTTCCCTAACTAATTATTA+GGG + chr4_3:71319618-71319637 Msa0660480:intron 20.0%
!!! ATGTACTTGTTAGAAATTAA+AGG - chr4_3:71319732-71319751 None:intergenic 20.0%
!! TACATATTCATTCAACAAAT+GGG + chr4_3:71319747-71319766 Msa0660480:intron 20.0%
!!! TCATTATTTTCTCTTGAATA+TGG - chr4_3:71319821-71319840 None:intergenic 20.0%
!! AATTGTTAATATTGTGATGT+AGG + chr4_3:71319877-71319896 Msa0660480:intron 20.0%
!!! AATAAAGATAGTTTTTTCCA+TGG - chr4_3:71320329-71320348 None:intergenic 20.0%
!!! TAAATTTTGTGAAAGATTTC+TGG + chr4_3:71320435-71320454 Msa0660480:three_prime_UTR 20.0%
!! GTTTGATGTACTAATAATTT+GGG - chr4_3:71318996-71319015 None:intergenic 20.0%
!! AGTTTGATGTACTAATAATT+TGG - chr4_3:71318997-71319016 None:intergenic 20.0%
!!! AGAGAAAAAAATTTGTTCTA+GGG - chr4_3:71319099-71319118 None:intergenic 20.0%
!!! AAGAGAAAAAAATTTGTTCT+AGG - chr4_3:71319100-71319119 None:intergenic 20.0%
!!! TTTTTCCCTAACTAATTATT+AGG + chr4_3:71319617-71319636 Msa0660480:intron 20.0%
!!! TTTTCCCTAACTAATTATTA+GGG + chr4_3:71319618-71319637 Msa0660480:intron 20.0%
!!! ATGTACTTGTTAGAAATTAA+AGG - chr4_3:71319732-71319751 None:intergenic 20.0%
!! TACATATTCATTCAACAAAT+GGG + chr4_3:71319747-71319766 Msa0660480:intron 20.0%
!!! TCATTATTTTCTCTTGAATA+TGG - chr4_3:71319821-71319840 None:intergenic 20.0%
!! AATTGTTAATATTGTGATGT+AGG + chr4_3:71319877-71319896 Msa0660480:intron 20.0%
!!! AATAAAGATAGTTTTTTCCA+TGG - chr4_3:71320329-71320348 None:intergenic 20.0%
!!! TAAATTTTGTGAAAGATTTC+TGG + chr4_3:71320435-71320454 Msa0660480:three_prime_UTR 20.0%
! TAGCTTGAAAGAAAATGAAA+AGG - chr4_3:71318889-71318908 None:intergenic 25.0%
!! TTTTCATTTTCTTTCAAGCT+AGG + chr4_3:71318888-71318907 Msa0660480:five_prime_UTR 25.0%
! TCTGAAAATTAAGCTAGAAA+AGG + chr4_3:71319137-71319156 Msa0660480:five_prime_UTR 25.0%
!! GGATTCAATTCAAGATTTTA+TGG + chr4_3:71319171-71319190 Msa0660480:CDS 25.0%
!!! CTCATTTCATGTTTTTTTGT+TGG - chr4_3:71319448-71319467 None:intergenic 25.0%
!!! GTTTTGGTGAATCAATATTT+TGG + chr4_3:71319503-71319522 Msa0660480:intron 25.0%
! CATACCCTAATAATTAGTTA+GGG - chr4_3:71319625-71319644 None:intergenic 25.0%
! TCATACCCTAATAATTAGTT+AGG - chr4_3:71319626-71319645 None:intergenic 25.0%
! GTACATATTCATTCAACAAA+TGG + chr4_3:71319746-71319765 Msa0660480:intron 25.0%
! CAAAAAAATGTTGCAAGATT+AGG - chr4_3:71319853-71319872 None:intergenic 25.0%
!!! CTAATCTTGCAACATTTTTT+TGG + chr4_3:71319851-71319870 Msa0660480:intron 25.0%
! TTAGTTATGAGAAGAAGAAA+AGG + chr4_3:71320147-71320166 Msa0660480:CDS 25.0%
! AGTTAACTAATTGATGTACA+TGG + chr4_3:71320619-71320638 Msa0660480:three_prime_UTR 25.0%
! TTAAATGAGGATATGTTATG+GGG - chr4_3:71318737-71318756 None:intergenic 25.0%
! GTTAAATGAGGATATGTTAT+GGG - chr4_3:71318738-71318757 None:intergenic 25.0%
! TGTTAAATGAGGATATGTTA+TGG - chr4_3:71318739-71318758 None:intergenic 25.0%
! TAGCTTGAAAGAAAATGAAA+AGG - chr4_3:71318889-71318908 None:intergenic 25.0%
!! TTTTCATTTTCTTTCAAGCT+AGG + chr4_3:71318888-71318907 Msa0660480:five_prime_UTR 25.0%
! TCTGAAAATTAAGCTAGAAA+AGG + chr4_3:71319137-71319156 Msa0660480:five_prime_UTR 25.0%
!! GGATTCAATTCAAGATTTTA+TGG + chr4_3:71319171-71319190 Msa0660480:CDS 25.0%
!!! CTCATTTCATGTTTTTTTGT+TGG - chr4_3:71319448-71319467 None:intergenic 25.0%
!!! GTTTTGGTGAATCAATATTT+TGG + chr4_3:71319503-71319522 Msa0660480:intron 25.0%
! CATACCCTAATAATTAGTTA+GGG - chr4_3:71319625-71319644 None:intergenic 25.0%
! TCATACCCTAATAATTAGTT+AGG - chr4_3:71319626-71319645 None:intergenic 25.0%
! GTACATATTCATTCAACAAA+TGG + chr4_3:71319746-71319765 Msa0660480:intron 25.0%
! CAAAAAAATGTTGCAAGATT+AGG - chr4_3:71319853-71319872 None:intergenic 25.0%
!!! CTAATCTTGCAACATTTTTT+TGG + chr4_3:71319851-71319870 Msa0660480:intron 25.0%
! TTAGTTATGAGAAGAAGAAA+AGG + chr4_3:71320147-71320166 Msa0660480:CDS 25.0%
! AGTTAACTAATTGATGTACA+TGG + chr4_3:71320619-71320638 Msa0660480:three_prime_UTR 25.0%
TTGAAGAGATAGATGGTATA+TGG - chr4_3:71318801-71318820 None:intergenic 30.0%
AGAGAAATTGAAGAGATAGA+TGG - chr4_3:71318808-71318827 None:intergenic 30.0%
AAAGGAAACAAACTCTGATT+TGG - chr4_3:71318871-71318890 None:intergenic 30.0%
! TTCTCTTTTCAAAACAACAC+TGG + chr4_3:71319113-71319132 Msa0660480:five_prime_UTR 30.0%
CTAGAAAAGGATTCAATCTA+TGG + chr4_3:71319150-71319169 Msa0660480:exon 30.0%
!!! GTTTTTTTGTTGGTATGTAC+CGG - chr4_3:71319438-71319457 None:intergenic 30.0%
!! TTGGTGAATCAATATTTTGG+TGG + chr4_3:71319506-71319525 Msa0660480:intron 30.0%
ACATACAAAAGATCGATCTA+GGG + chr4_3:71319770-71319789 Msa0660480:intron 30.0%
AATATTGTGATGTAGGTACT+TGG + chr4_3:71319884-71319903 Msa0660480:intron 30.0%
ATAACTAATACCTCTTGCTT+TGG - chr4_3:71320135-71320154 None:intergenic 30.0%
TTGAAGAGATAGATGGTATA+TGG - chr4_3:71318801-71318820 None:intergenic 30.0%
AGAGAAATTGAAGAGATAGA+TGG - chr4_3:71318808-71318827 None:intergenic 30.0%
AAAGGAAACAAACTCTGATT+TGG - chr4_3:71318871-71318890 None:intergenic 30.0%
! TTCTCTTTTCAAAACAACAC+TGG + chr4_3:71319113-71319132 Msa0660480:five_prime_UTR 30.0%
CTAGAAAAGGATTCAATCTA+TGG + chr4_3:71319150-71319169 Msa0660480:exon 30.0%
!!! GTTTTTTTGTTGGTATGTAC+CGG - chr4_3:71319438-71319457 None:intergenic 30.0%
!! TTGGTGAATCAATATTTTGG+TGG + chr4_3:71319506-71319525 Msa0660480:intron 30.0%
ACATACAAAAGATCGATCTA+GGG + chr4_3:71319770-71319789 Msa0660480:intron 30.0%
AATATTGTGATGTAGGTACT+TGG + chr4_3:71319884-71319903 Msa0660480:intron 30.0%
ATAACTAATACCTCTTGCTT+TGG - chr4_3:71320135-71320154 None:intergenic 30.0%
GAGATAGATGGTATATGGTA+TGG - chr4_3:71318796-71318815 None:intergenic 35.0%
!! GGTTGATTGATTTTACAGAC+TGG - chr4_3:71318850-71318869 None:intergenic 35.0%
CTCATGTTCTTGAATTTCTC+CGG + chr4_3:71319416-71319435 Msa0660480:CDS 35.0%
AAAGAACAAGAGATGTTTCC+AGG - chr4_3:71319569-71319588 None:intergenic 35.0%
GACATACAAAAGATCGATCT+AGG + chr4_3:71319769-71319788 Msa0660480:intron 35.0%
! GAACTTTTGTCTTGCCAAAT+TGG - chr4_3:71319911-71319930 None:intergenic 35.0%
TGTCCATAGAATGGACAAAT+CGG - chr4_3:71319939-71319958 None:intergenic 35.0%
ATGTCCATTAAGACAAGCTT+GGG + chr4_3:71319983-71320002 Msa0660480:CDS 35.0%
AAGCCAGAAACAAATCCATT+TGG + chr4_3:71320062-71320081 Msa0660480:CDS 35.0%
ATGGCTATTCTCATATTCCA+TGG + chr4_3:71320309-71320328 Msa0660480:three_prime_UTR 35.0%
AAGTACTAGTAGTAGCTAGT+AGG + chr4_3:71320388-71320407 Msa0660480:three_prime_UTR 35.0%
TAGTAGTAGCTAGTAGGTTT+AGG + chr4_3:71320394-71320413 Msa0660480:three_prime_UTR 35.0%
TAGTTGAAGTAGCAGAAAAG+TGG + chr4_3:71320581-71320600 Msa0660480:three_prime_UTR 35.0%
CTAATTGATGTACATGGAGA+AGG + chr4_3:71320625-71320644 Msa0660480:three_prime_UTR 35.0%
GAGATAGATGGTATATGGTA+TGG - chr4_3:71318796-71318815 None:intergenic 35.0%
!! GGTTGATTGATTTTACAGAC+TGG - chr4_3:71318850-71318869 None:intergenic 35.0%
CTCATGTTCTTGAATTTCTC+CGG + chr4_3:71319416-71319435 Msa0660480:CDS 35.0%
AAAGAACAAGAGATGTTTCC+AGG - chr4_3:71319569-71319588 None:intergenic 35.0%
GACATACAAAAGATCGATCT+AGG + chr4_3:71319769-71319788 Msa0660480:intron 35.0%
! GAACTTTTGTCTTGCCAAAT+TGG - chr4_3:71319911-71319930 None:intergenic 35.0%
TGTCCATAGAATGGACAAAT+CGG - chr4_3:71319939-71319958 None:intergenic 35.0%
ATGTCCATTAAGACAAGCTT+GGG + chr4_3:71319983-71320002 Msa0660480:CDS 35.0%
AAGCCAGAAACAAATCCATT+TGG + chr4_3:71320062-71320081 Msa0660480:CDS 35.0%
ATGGCTATTCTCATATTCCA+TGG + chr4_3:71320309-71320328 Msa0660480:three_prime_UTR 35.0%
AAGTACTAGTAGTAGCTAGT+AGG + chr4_3:71320388-71320407 Msa0660480:three_prime_UTR 35.0%
TAGTAGTAGCTAGTAGGTTT+AGG + chr4_3:71320394-71320413 Msa0660480:three_prime_UTR 35.0%
TAGTTGAAGTAGCAGAAAAG+TGG + chr4_3:71320581-71320600 Msa0660480:three_prime_UTR 35.0%
CTAATTGATGTACATGGAGA+AGG + chr4_3:71320625-71320644 Msa0660480:three_prime_UTR 35.0%
AGAGATTGAATCCTGAAACC+TGG + chr4_3:71319548-71319567 Msa0660480:intron 40.0%
AAGAGATGTTTCCAGGTTTC+AGG - chr4_3:71319562-71319581 None:intergenic 40.0%
TGTAGGTACTTGGACCAATT+TGG + chr4_3:71319894-71319913 Msa0660480:intron 40.0%
!! ACACCGATTTGTCCATTCTA+TGG + chr4_3:71319933-71319952 Msa0660480:CDS 40.0%
! TGTCTTAATGGACATGGACA+TGG - chr4_3:71319978-71319997 None:intergenic 40.0%
! CAAGCTTGTCTTAATGGACA+TGG - chr4_3:71319984-71320003 None:intergenic 40.0%
CATGTCCATTAAGACAAGCT+TGG + chr4_3:71319982-71320001 Msa0660480:CDS 40.0%
TGTCCATTAAGACAAGCTTG+GGG + chr4_3:71319984-71320003 Msa0660480:CDS 40.0%
! ATGCACTGATAGGTCGTTTA+AGG + chr4_3:71320015-71320034 Msa0660480:CDS 40.0%
! TGCACTGATAGGTCGTTTAA+GGG + chr4_3:71320016-71320035 Msa0660480:CDS 40.0%
!! CAGCTTTTGAGGAAAATGGA+GGG + chr4_3:71320039-71320058 Msa0660480:CDS 40.0%
! GCACCAAATGGATTTGTTTC+TGG - chr4_3:71320068-71320087 None:intergenic 40.0%
TGTTAGACTTTACCTTCGTG+AGG + chr4_3:71320094-71320113 Msa0660480:CDS 40.0%
GATCTTCAATCCAAAGCAAG+AGG + chr4_3:71320122-71320141 Msa0660480:CDS 40.0%
AGAGGCAATTGCAATTGCAT+TGG - chr4_3:71320233-71320252 None:intergenic 40.0%
! TGGTGATGATGAAGATGAAG+AGG - chr4_3:71320251-71320270 None:intergenic 40.0%
!! TTTTATTGAGTTGCACCTGG+AGG - chr4_3:71320290-71320309 None:intergenic 40.0%
!! CCATTTTATTGAGTTGCACC+TGG - chr4_3:71320293-71320312 None:intergenic 40.0%
CCAGGTGCAACTCAATAAAA+TGG + chr4_3:71320290-71320309 Msa0660480:exon 40.0%
TTGAAGTAGCAGAAAAGTGG+TGG + chr4_3:71320584-71320603 Msa0660480:three_prime_UTR 40.0%
AGAGATTGAATCCTGAAACC+TGG + chr4_3:71319548-71319567 Msa0660480:intron 40.0%
AAGAGATGTTTCCAGGTTTC+AGG - chr4_3:71319562-71319581 None:intergenic 40.0%
TGTAGGTACTTGGACCAATT+TGG + chr4_3:71319894-71319913 Msa0660480:intron 40.0%
!! ACACCGATTTGTCCATTCTA+TGG + chr4_3:71319933-71319952 Msa0660480:CDS 40.0%
! TGTCTTAATGGACATGGACA+TGG - chr4_3:71319978-71319997 None:intergenic 40.0%
! CAAGCTTGTCTTAATGGACA+TGG - chr4_3:71319984-71320003 None:intergenic 40.0%
CATGTCCATTAAGACAAGCT+TGG + chr4_3:71319982-71320001 Msa0660480:CDS 40.0%
TGTCCATTAAGACAAGCTTG+GGG + chr4_3:71319984-71320003 Msa0660480:CDS 40.0%
! ATGCACTGATAGGTCGTTTA+AGG + chr4_3:71320015-71320034 Msa0660480:CDS 40.0%
! TGCACTGATAGGTCGTTTAA+GGG + chr4_3:71320016-71320035 Msa0660480:CDS 40.0%
!! CAGCTTTTGAGGAAAATGGA+GGG + chr4_3:71320039-71320058 Msa0660480:CDS 40.0%
! GCACCAAATGGATTTGTTTC+TGG - chr4_3:71320068-71320087 None:intergenic 40.0%
TGTTAGACTTTACCTTCGTG+AGG + chr4_3:71320094-71320113 Msa0660480:CDS 40.0%
GATCTTCAATCCAAAGCAAG+AGG + chr4_3:71320122-71320141 Msa0660480:CDS 40.0%
AGAGGCAATTGCAATTGCAT+TGG - chr4_3:71320233-71320252 None:intergenic 40.0%
! TGGTGATGATGAAGATGAAG+AGG - chr4_3:71320251-71320270 None:intergenic 40.0%
!! TTTTATTGAGTTGCACCTGG+AGG - chr4_3:71320290-71320309 None:intergenic 40.0%
!! CCATTTTATTGAGTTGCACC+TGG - chr4_3:71320293-71320312 None:intergenic 40.0%
CCAGGTGCAACTCAATAAAA+TGG + chr4_3:71320290-71320309 Msa0660480:exon 40.0%
TTGAAGTAGCAGAAAAGTGG+TGG + chr4_3:71320584-71320603 Msa0660480:three_prime_UTR 40.0%
TCTTGCTTCACCACTTGAAG+AGG + chr4_3:71318769-71318788 Msa0660480:five_prime_UTR 45.0%
ATGGTATGGACCTCTTCAAG+TGG - chr4_3:71318782-71318801 None:intergenic 45.0%
GAGGGAGTGAGAATATAGGA+TGG - chr4_3:71319049-71319068 None:intergenic 45.0%
TCACTCTGGTCACTGTCTTT+AGG + chr4_3:71319071-71319090 Msa0660480:five_prime_UTR 45.0%
!! TAGTTATGGTGCTGTTGGTG+AGG - chr4_3:71319225-71319244 None:intergenic 45.0%
AGGTGAAGAGCTACCAACTA+TGG - chr4_3:71319278-71319297 None:intergenic 45.0%
!!! GGCGTTTCTGGTTTTCATAG+CGG - chr4_3:71319324-71319343 None:intergenic 45.0%
GGGTTTGGATGTCCATAGAA+TGG - chr4_3:71319948-71319967 None:intergenic 45.0%
CTACCCCAAGCTTGTCTTAA+TGG - chr4_3:71319990-71320009 None:intergenic 45.0%
! GGTAGTCTTGATGCACTGAT+AGG + chr4_3:71320005-71320024 Msa0660480:CDS 45.0%
! TCGTTTAAGGGCAGCTTTTG+AGG + chr4_3:71320028-71320047 Msa0660480:CDS 45.0%
!! AGGGCAGCTTTTGAGGAAAA+TGG + chr4_3:71320035-71320054 Msa0660480:CDS 45.0%
!! GCAGCTTTTGAGGAAAATGG+AGG + chr4_3:71320038-71320057 Msa0660480:CDS 45.0%
! CTAACAGCTCTAGCACCAAA+TGG - chr4_3:71320080-71320099 None:intergenic 45.0%
! TGTTGTGGTTGTTGTTGTGG+TGG - chr4_3:71320179-71320198 None:intergenic 45.0%
! AGCTGTTGTGGTTGTTGTTG+TGG - chr4_3:71320182-71320201 None:intergenic 45.0%
AAGTAGCAGAAAAGTGGTGG+TGG + chr4_3:71320587-71320606 Msa0660480:three_prime_UTR 45.0%
TCTTGCTTCACCACTTGAAG+AGG + chr4_3:71318769-71318788 Msa0660480:five_prime_UTR 45.0%
ATGGTATGGACCTCTTCAAG+TGG - chr4_3:71318782-71318801 None:intergenic 45.0%
GAGGGAGTGAGAATATAGGA+TGG - chr4_3:71319049-71319068 None:intergenic 45.0%
TCACTCTGGTCACTGTCTTT+AGG + chr4_3:71319071-71319090 Msa0660480:five_prime_UTR 45.0%
!! TAGTTATGGTGCTGTTGGTG+AGG - chr4_3:71319225-71319244 None:intergenic 45.0%
AGGTGAAGAGCTACCAACTA+TGG - chr4_3:71319278-71319297 None:intergenic 45.0%
!!! GGCGTTTCTGGTTTTCATAG+CGG - chr4_3:71319324-71319343 None:intergenic 45.0%
GGGTTTGGATGTCCATAGAA+TGG - chr4_3:71319948-71319967 None:intergenic 45.0%
CTACCCCAAGCTTGTCTTAA+TGG - chr4_3:71319990-71320009 None:intergenic 45.0%
! GGTAGTCTTGATGCACTGAT+AGG + chr4_3:71320005-71320024 Msa0660480:CDS 45.0%
! TCGTTTAAGGGCAGCTTTTG+AGG + chr4_3:71320028-71320047 Msa0660480:CDS 45.0%
!! AGGGCAGCTTTTGAGGAAAA+TGG + chr4_3:71320035-71320054 Msa0660480:CDS 45.0%
!! GCAGCTTTTGAGGAAAATGG+AGG + chr4_3:71320038-71320057 Msa0660480:CDS 45.0%
! CTAACAGCTCTAGCACCAAA+TGG - chr4_3:71320080-71320099 None:intergenic 45.0%
! TGTTGTGGTTGTTGTTGTGG+TGG - chr4_3:71320179-71320198 None:intergenic 45.0%
! AGCTGTTGTGGTTGTTGTTG+TGG - chr4_3:71320182-71320201 None:intergenic 45.0%
AAGTAGCAGAAAAGTGGTGG+TGG + chr4_3:71320587-71320606 Msa0660480:three_prime_UTR 45.0%
GAGCGAGGGAGTGAGAATAT+AGG - chr4_3:71319053-71319072 None:intergenic 50.0%
!! GTGGGTAGTTATGGTGCTGT+TGG - chr4_3:71319230-71319249 None:intergenic 50.0%
GTTGCTGCTGTGGGTAGTTA+TGG - chr4_3:71319239-71319258 None:intergenic 50.0%
!! GATGGTGTTGTTGCTGCTGT+GGG - chr4_3:71319248-71319267 None:intergenic 50.0%
!! TGATGGTGTTGTTGCTGCTG+TGG - chr4_3:71319249-71319268 None:intergenic 50.0%
ACCATCAGCAACGCCATAGT+TGG + chr4_3:71319262-71319281 Msa0660480:CDS 50.0%
ACCAACTATGGCGTTGCTGA+TGG - chr4_3:71319266-71319285 None:intergenic 50.0%
!! GTTGGTAGCTCTTCACCTTC+TGG + chr4_3:71319280-71319299 Msa0660480:CDS 50.0%
! GTTTTCATAGCGGCTGCTTG+TGG - chr4_3:71319314-71319333 None:intergenic 50.0%
AAAACCAGAAACGCCGTGAC+TGG + chr4_3:71319329-71319348 Msa0660480:CDS 50.0%
!! GGCCAAAAGTGTTCCAGTCA+CGG - chr4_3:71319345-71319364 None:intergenic 50.0%
GACGGTGATTCTTGAGGTAC+TGG - chr4_3:71319366-71319385 None:intergenic 50.0%
TAGAGAGGGAAAGAGGAGGA+CGG - chr4_3:71319384-71319403 None:intergenic 50.0%
CATCTAGAGAGGGAAAGAGG+AGG - chr4_3:71319388-71319407 None:intergenic 50.0%
CTGCATCTAGAGAGGGAAAG+AGG - chr4_3:71319391-71319410 None:intergenic 50.0%
CTTTCCCTCTCTAGATGCAG+CGG + chr4_3:71319391-71319410 Msa0660480:CDS 50.0%
AATGGACATGGACATGGTGC+TGG - chr4_3:71319972-71319991 None:intergenic 50.0%
GAAGATCACGAACCTCACGA+AGG - chr4_3:71320109-71320128 None:intergenic 50.0%
TGCTGAAGCTGAAGCTGTTG+TGG - chr4_3:71320194-71320213 None:intergenic 50.0%
GAGGTGGAAGCTGATGTTGA+TGG - chr4_3:71320271-71320290 None:intergenic 50.0%
! TATTGAGTTGCACCTGGAGG+TGG - chr4_3:71320287-71320306 None:intergenic 50.0%
GAGCGAGGGAGTGAGAATAT+AGG - chr4_3:71319053-71319072 None:intergenic 50.0%
!! GTGGGTAGTTATGGTGCTGT+TGG - chr4_3:71319230-71319249 None:intergenic 50.0%
GTTGCTGCTGTGGGTAGTTA+TGG - chr4_3:71319239-71319258 None:intergenic 50.0%
!! GATGGTGTTGTTGCTGCTGT+GGG - chr4_3:71319248-71319267 None:intergenic 50.0%
!! TGATGGTGTTGTTGCTGCTG+TGG - chr4_3:71319249-71319268 None:intergenic 50.0%
ACCATCAGCAACGCCATAGT+TGG + chr4_3:71319262-71319281 Msa0660480:CDS 50.0%
ACCAACTATGGCGTTGCTGA+TGG - chr4_3:71319266-71319285 None:intergenic 50.0%
!! GTTGGTAGCTCTTCACCTTC+TGG + chr4_3:71319280-71319299 Msa0660480:CDS 50.0%
! GTTTTCATAGCGGCTGCTTG+TGG - chr4_3:71319314-71319333 None:intergenic 50.0%
AAAACCAGAAACGCCGTGAC+TGG + chr4_3:71319329-71319348 Msa0660480:CDS 50.0%
!! GGCCAAAAGTGTTCCAGTCA+CGG - chr4_3:71319345-71319364 None:intergenic 50.0%
GACGGTGATTCTTGAGGTAC+TGG - chr4_3:71319366-71319385 None:intergenic 50.0%
TAGAGAGGGAAAGAGGAGGA+CGG - chr4_3:71319384-71319403 None:intergenic 50.0%
CATCTAGAGAGGGAAAGAGG+AGG - chr4_3:71319388-71319407 None:intergenic 50.0%
CTGCATCTAGAGAGGGAAAG+AGG - chr4_3:71319391-71319410 None:intergenic 50.0%
CTTTCCCTCTCTAGATGCAG+CGG + chr4_3:71319391-71319410 Msa0660480:CDS 50.0%
AATGGACATGGACATGGTGC+TGG - chr4_3:71319972-71319991 None:intergenic 50.0%
GAAGATCACGAACCTCACGA+AGG - chr4_3:71320109-71320128 None:intergenic 50.0%
TGCTGAAGCTGAAGCTGTTG+TGG - chr4_3:71320194-71320213 None:intergenic 50.0%
GAGGTGGAAGCTGATGTTGA+TGG - chr4_3:71320271-71320290 None:intergenic 50.0%
! TATTGAGTTGCACCTGGAGG+TGG - chr4_3:71320287-71320306 None:intergenic 50.0%
ACAGTGACCAGAGTGAGCGA+GGG - chr4_3:71319067-71319086 None:intergenic 55.0%
CTTGTGGTGGTAGAGCCAGA+AGG - chr4_3:71319298-71319317 None:intergenic 55.0%
TTCATAGCGGCTGCTTGTGG+TGG - chr4_3:71319311-71319330 None:intergenic 55.0%
TGTTCCAGTCACGGCGTTTC+TGG - chr4_3:71319336-71319355 None:intergenic 55.0%
! CGCCGTGACTGGAACACTTT+TGG + chr4_3:71319340-71319359 Msa0660480:CDS 55.0%
GAGGAGGACGGTGATTCTTG+AGG - chr4_3:71319372-71319391 None:intergenic 55.0%
AGCACCGCTGCATCTAGAGA+GGG - chr4_3:71319398-71319417 None:intergenic 55.0%
! GACATGGACATGGTGCTGGA+GGG - chr4_3:71319968-71319987 None:intergenic 55.0%
CAACATCAGCTTCCACCTCC+AGG + chr4_3:71320272-71320291 Msa0660480:CDS 55.0%
ACAGTGACCAGAGTGAGCGA+GGG - chr4_3:71319067-71319086 None:intergenic 55.0%
CTTGTGGTGGTAGAGCCAGA+AGG - chr4_3:71319298-71319317 None:intergenic 55.0%
TTCATAGCGGCTGCTTGTGG+TGG - chr4_3:71319311-71319330 None:intergenic 55.0%
TGTTCCAGTCACGGCGTTTC+TGG - chr4_3:71319336-71319355 None:intergenic 55.0%
! CGCCGTGACTGGAACACTTT+TGG + chr4_3:71319340-71319359 Msa0660480:CDS 55.0%
GAGGAGGACGGTGATTCTTG+AGG - chr4_3:71319372-71319391 None:intergenic 55.0%
AGCACCGCTGCATCTAGAGA+GGG - chr4_3:71319398-71319417 None:intergenic 55.0%
! GACATGGACATGGTGCTGGA+GGG - chr4_3:71319968-71319987 None:intergenic 55.0%
CAACATCAGCTTCCACCTCC+AGG + chr4_3:71320272-71320291 Msa0660480:CDS 55.0%
TCTCACTCCCTCGCTCACTC+TGG + chr4_3:71319057-71319076 Msa0660480:five_prime_UTR 60.0%
GACAGTGACCAGAGTGAGCG+AGG - chr4_3:71319068-71319087 None:intergenic 60.0%
! GAGCACCGCTGCATCTAGAG+AGG - chr4_3:71319399-71319418 None:intergenic 60.0%
! GGACATGGTGCTGGAGGGTT+TGG - chr4_3:71319963-71319982 None:intergenic 60.0%
! GGACATGGACATGGTGCTGG+AGG - chr4_3:71319969-71319988 None:intergenic 60.0%
TCTCACTCCCTCGCTCACTC+TGG + chr4_3:71319057-71319076 Msa0660480:five_prime_UTR 60.0%
GACAGTGACCAGAGTGAGCG+AGG - chr4_3:71319068-71319087 None:intergenic 60.0%
! GAGCACCGCTGCATCTAGAG+AGG - chr4_3:71319399-71319418 None:intergenic 60.0%
! GGACATGGTGCTGGAGGGTT+TGG - chr4_3:71319963-71319982 None:intergenic 60.0%
! GGACATGGACATGGTGCTGG+AGG - chr4_3:71319969-71319988 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 71318737 71320659 71318737 ID=Msa0660480;Name=Msa0660480
chr4_3 mRNA 71318737 71320659 71318737 ID=Msa0660480-mRNA-1;Parent=Msa0660480;Name=Msa0660480-mRNA-1;_AED=0.03;_eAED=0.03;_QI=432|1|1|1|1|1|2|352|225
chr4_3 exon 71318737 71319437 71318737 ID=Msa0660480-mRNA-1:exon:21311;Parent=Msa0660480-mRNA-1
chr4_3 exon 71319899 71320659 71319899 ID=Msa0660480-mRNA-1:exon:21312;Parent=Msa0660480-mRNA-1
chr4_3 five_prime_UTR 71318737 71319168 71318737 ID=Msa0660480-mRNA-1:five_prime_utr;Parent=Msa0660480-mRNA-1
chr4_3 CDS 71319169 71319437 71319169 ID=Msa0660480-mRNA-1:cds;Parent=Msa0660480-mRNA-1
chr4_3 CDS 71319899 71320307 71319899 ID=Msa0660480-mRNA-1:cds;Parent=Msa0660480-mRNA-1
chr4_3 three_prime_UTR 71320308 71320659 71320308 ID=Msa0660480-mRNA-1:three_prime_utr;Parent=Msa0660480-mRNA-1
Gene Sequence

>Msa0660480

ATGGATTCAATTCAAGATTTTATGGATTCATGTAACTCTGACAACAGTTGCAGCCTCACCAACAGCACCATAACTACCCACAGCAGCAACAACACCATCAGCAACGCCATAGTTGGTAGCTCTTCACCTTCTGGCTCTACCACCACAAGCAGCCGCTATGAAAACCAGAAACGCCGTGACTGGAACACTTTTGGCCAGTACCTCAAGAATCACCGTCCTCCTCTTTCCCTCTCTAGATGCAGCGGTGCTCATGTTCTTGAATTTCTCCGGTACTTGGACCAATTTGGCAAGACAAAAGTTCACACACCGATTTGTCCATTCTATGGACATCCAAACCCTCCAGCACCATGTCCATGTCCATTAAGACAAGCTTGGGGTAGTCTTGATGCACTGATAGGTCGTTTAAGGGCAGCTTTTGAGGAAAATGGAGGGAAGCCAGAAACAAATCCATTTGGTGCTAGAGCTGTTAGACTTTACCTTCGTGAGGTTCGTGATCTTCAATCCAAAGCAAGAGGTATTAGTTATGAGAAGAAGAAAAGGAAGCGTCCACCACAACAACAACCACAACAGCTTCAGCTTCAGCAGCAACAACAGCAACAACCAATGCAATTGCAATTGCCTCTTCATCTTCATCATCACCATCAACATCAGCTTCCACCTCCAGGTGCAACTCAATAA
Protein sequence

>Msa0660480

MDSIQDFMDSCNSDNSCSLTNSTITTHSSNNTISNAIVGSSSPSGSTTTSSRYENQKRRDWNTFGQYLKNHRPPLSLSRCSGAHVLEFLRYLDQFGKTKVHTPICPFYGHPNPPAPCPCPLRQAWGSLDALIGRLRAAFEENGGKPETNPFGARAVRLYLREVRDLQSKARGISYEKKKRKRPPQQQPQQLQLQQQQQQQPMQLQLPLHLHHHHQHQLPPPGATQ*