AlfalfaGEDB | Msa0124220

M. sativa cultivar zhongmu-4 / Msa0124220

KEGG: K08967|K09419
GO: GO:0003674 GO:0003700 GO:0005488 GO:0005515 GO:0006355 GO:0006950 GO:0008150 GO:0009266 GO:0009314 GO:0009408 GO:0009416 GO:0009628 GO:0009642 GO:0009644 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0032268 GO:0042802 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051246 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00447.20

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0124220	sp\|Q9LUH8\|HFA6B_ARATH	48.358	335	133	8	10	305	41	374	7.73e-92	284

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0124220	G7ICS1	86.234	385	39	5	1	384	1	372	0.0	647

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TFs/TRs:

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Gene ID	Type	Classification
Msa0124220	TF	HSF

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0028510	Msa0124220	0.845872	3.095932e-59	-8.615850e-47
Msa0124220	Msa0168600	0.803330	3.669848e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0124220	MtrunA17_Chr1g0185131	88.439	346	32	4	1	345	4	342	0.0	601
Msa0124220	MtrunA17_Chr7g0260331	55.331	347	114	9	1	320	4	336	2.25e-105	315
Msa0124220	MtrunA17_Chr1g0204741	48.243	313	121	8	17	316	27	311	3.07e-80	249
Msa0124220	MtrunA17_Chr3g0135831	45.000	300	150	6	17	305	33	328	3.91e-75	238
Msa0124220	MtrunA17_Chr4g0039891	50.230	217	94	2	22	226	4	218	9.87e-67	219
Msa0124220	MtrunA17_Chr5g0410881	50.685	219	88	5	28	230	30	244	7.19e-66	217
Msa0124220	MtrunA17_Chr4g0071051	46.875	224	106	4	17	233	88	305	2.40e-62	206
Msa0124220	MtrunA17_Chr6g0467041	44.000	200	104	4	30	226	22	216	2.01e-47	164
Msa0124220	MtrunA17_Chr8g0379701	38.911	257	132	7	30	265	12	264	2.21e-47	166
Msa0124220	MtrunA17_Chr8g0392001	40.164	244	127	5	30	254	22	265	2.37e-47	165

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0124220	AT3G22830.1	48.358	335	133	8	10	305	41	374	7.86e-93	284
Msa0124220	AT3G51910.1	52.962	287	90	9	21	305	20	263	4.06e-86	263
Msa0124220	AT3G63350.1	44.136	324	125	7	15	336	13	282	2.32e-79	246
Msa0124220	AT2G26150.1	50.230	217	102	2	17	227	31	247	9.51e-72	228
Msa0124220	AT2G26150.4	50.230	217	102	2	17	227	31	247	9.51e-72	228
Msa0124220	AT1G32330.2	50.472	212	78	3	15	205	22	227	2.10e-64	213
Msa0124220	AT1G32330.1	50.472	212	78	3	15	205	22	227	2.26e-64	213
Msa0124220	AT5G43840.1	41.522	289	142	7	17	294	6	278	7.58e-64	206
Msa0124220	AT4G17750.1	43.561	264	109	5	12	244	31	285	1.03e-62	209
Msa0124220	AT5G16820.1	49.123	228	95	4	11	226	16	234	1.73e-61	206

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Find 83 sgRNAs with CRISPR-Local

Find 232 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACAACCTTGTCCTTGTT+GGG	0.399773	1_3:-51882106	None:intergenic
AAACTCATATATGCAATCCT+TGG	0.512900	1_3:-51883182	None:intergenic
AAAGAGTGCTTGATGATGAA+GGG	0.670867	1_3:+51882592	Msa0124220:CDS
AACAAGTCCTTCATTTGGTT+GGG	0.266336	1_3:-51881608	None:intergenic
AACCACTGAGTCAAGAATTT+AGG	0.293001	1_3:+51882709	Msa0124220:CDS
AAGAGAAAGCTTGTTGAACA+AGG	0.580615	1_3:+51882384	Msa0124220:CDS
AATGTTAGCTATGGAAATGC+AGG	0.499978	1_3:+51882494	Msa0124220:CDS
AATGTTTAGTATGAAATTTG+AGG	0.507525	1_3:+51882629	Msa0124220:CDS
AATTCTTAGACATATGTACT+CGG	0.641403	1_3:+51881546	Msa0124220:exon
ACAACAGAAAGAGAAGAAAA+AGG	0.330208	1_3:+51882332	Msa0124220:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATTTCAACAAGTCCTTCATT+TGG	-	chr1_3:51881616-51881635	None:intergenic	30.0%
GTACCAACCATATAATTTCA+TGG	+	chr1_3:51881686-51881705	Msa0124220:CDS	30.0%
GTTCCATGAAATTATATGGT+TGG	-	chr1_3:51881692-51881711	None:intergenic	30.0%
ATAATTTCATGGAACAGAAG+TGG	+	chr1_3:51881697-51881716	Msa0124220:CDS	30.0%
GAAAAATTGATCCAGATAGA+TGG	+	chr1_3:51881992-51882011	Msa0124220:CDS	30.0%
ATGCAGAAATTGATCGTTTA+AGG	+	chr1_3:51882136-51882155	Msa0124220:CDS	30.0%
ACAACAGAAAGAGAAGAAAA+AGG	+	chr1_3:51882332-51882351	Msa0124220:CDS	30.0%
TCATAATCACGTAACTCTAA+CGG	-	chr1_3:51882453-51882472	None:intergenic	30.0%
GAGTTACGTGATTATGAATT+TGG	+	chr1_3:51882456-51882475	Msa0124220:CDS	30.0%
GTTACGTGATTATGAATTTG+GGG	+	chr1_3:51882458-51882477	Msa0124220:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_3	gene	51881456	51883280	51881456	ID=Msa0124220;Name=Msa0124220
chr1_3	mRNA	51881456	51883280	51881456	ID=Msa0124220-mRNA-1;Parent=Msa0124220;Name=Msa0124220-mRNA-1;_AED=0.20;_eAED=0.20;_QI=103\|0.66\|0.5\|1\|1\|1\|4\|195\|387
chr1_3	exon	51881456	51881825	51881456	ID=Msa0124220-mRNA-1:exon:15836;Parent=Msa0124220-mRNA-1
chr1_3	exon	51881985	51882749	51881985	ID=Msa0124220-mRNA-1:exon:15837;Parent=Msa0124220-mRNA-1
chr1_3	exon	51882873	51882941	51882873	ID=Msa0124220-mRNA-1:exon:15838;Parent=Msa0124220-mRNA-1
chr1_3	exon	51883023	51883280	51883023	ID=Msa0124220-mRNA-1:exon:15839;Parent=Msa0124220-mRNA-1
chr1_3	five_prime_UTR	51881456	51881558	51881456	ID=Msa0124220-mRNA-1:five_prime_utr;Parent=Msa0124220-mRNA-1
chr1_3	CDS	51881559	51881825	51881559	ID=Msa0124220-mRNA-1:cds;Parent=Msa0124220-mRNA-1
chr1_3	CDS	51881985	51882749	51881985	ID=Msa0124220-mRNA-1:cds;Parent=Msa0124220-mRNA-1
chr1_3	CDS	51882873	51882941	51882873	ID=Msa0124220-mRNA-1:cds;Parent=Msa0124220-mRNA-1
chr1_3	CDS	51883023	51883085	51883023	ID=Msa0124220-mRNA-1:cds;Parent=Msa0124220-mRNA-1
chr1_3	three_prime_UTR	51883086	51883280	51883086	ID=Msa0124220-mRNA-1:three_prime_utr;Parent=Msa0124220-mRNA-1

Gene Sequence

>Msa0124220

ATGTACTCGGTGAAAGAAGAATACTTGGAACCAACGATTCCACCACCACCCCAACCAAATGAAGGACTTGTTGAAATTAGACCACAACCATTCCTCACAAAAACATTTGATGTAGTTGATGATCAAAGTACCAACCATATAATTTCATGGAACAGAAGTGGCACCAGTTTTGTGGTTTGGGATACACATGCCTTCTCCAATCATCTCCTTCCCAGATACTTCAAACACAATAATTTCTCAAGTTTTGTCAGACAATTAAACACTTATGGATTTAGAAAAATTGATCCAGATAGATGGGAATTTGCAAATGAAAGATTCCTGAGAGGGCAAAGACATCTATTGAGGAACATAAGAAGAAAGAAGGTACCTTCTCAGCCTATTCCTAATTCCCAACAAGGACAAGGTTGTTTTGGACTAGATGCAGAAATTGATCGTTTAAGGCAAGAGAAACACGTGTTGATGACGGAGCTTATGAGTCTTAGAAAGCAGCAATATAATACTACACTTTATATTCTACAGATGGAGCAAAGGTTAGAAGGGATTGAAATCAATCAGAAGAAAATGATGTCTTTCTTAGCTAGAGCTATGAAAAACCCTGTCTTCATTCACCAACTACAACAGAAAGAGAAGAAAAAGGAGCTTGAAGAATCCGCGACCATGACAAAAAAGAGAAAGCTTGTTGAACAAGGAACTAGACTAGGTATTGGACAATCAAGCAGTGTTAAAGTTGAGCCGTTAGAGTTACGTGATTATGAATTTGGGGTGTCGGAGTTGGAAATGTTAGCTATGGAAATGCAGGTTTTTGGAAGGGGGAAAATAATGGATAAAGAGGAAGTACATGAACCTGAGGCACTAGAATCACAAGAGAAGATGGAAAGAGTGCTTGATGATGAAGGGTTCTGGGAAGATCTAATGTTTAGTATGAAATTTGAGGGTAGATTTGACATTCCAACTTCTGAAGATAACAATGAGAATGAAGATGATTCAAGTGAACCACTGAGTCAAGAATTTAGGATTAACTCTTTTCATTCAGTTGCTGCTGGTGCCAGAACAAGATTCTTTGATAGCAAAATCTGTCATTATTTATGTCAAGGACTCAAGATTACTCAGAAGATATATGATGGACAAGAAGTAACTAATCTCCTAGAAAGCATAACTGAGTGA

Protein sequence

>Msa0124220

MYSVKEEYLEPTIPPPPQPNEGLVEIRPQPFLTKTFDVVDDQSTNHIISWNRSGTSFVVWDTHAFSNHLLPRYFKHNNFSSFVRQLNTYGFRKIDPDRWEFANERFLRGQRHLLRNIRRKKVPSQPIPNSQQGQGCFGLDAEIDRLRQEKHVLMTELMSLRKQQYNTTLYILQMEQRLEGIEINQKKMMSFLARAMKNPVFIHQLQQKEKKKELEESATMTKKRKLVEQGTRLGIGQSSSVKVEPLELRDYEFGVSELEMLAMEMQVFGRGKIMDKEEVHEPEALESQEKMERVLDDEGFWEDLMFSMKFEGRFDIPTSEDNNENEDDSSEPLSQEFRINSFHSVAAGARTRFFDSKICHYLCQGLKITQKIYDGQEVTNLLESITE*