AlfalfaGEDB | Msa0124650

M. sativa cultivar zhongmu-4 / Msa0124650

KEGG: K17108
GO: GO:0003674 GO:0003824 GO:0004553 GO:0005575 GO:0006082 GO:0006629 GO:0007275 GO:0007399 GO:0007417 GO:0008150 GO:0008152 GO:0008202 GO:0008206 GO:0008422 GO:0009056 GO:0009987 GO:0015926 GO:0016020 GO:0016021 GO:0016137 GO:0016139 GO:0016787 GO:0016798 GO:0019752 GO:0021953 GO:0021954 GO:0022008 GO:0030154 GO:0030182 GO:0031224 GO:0032501 GO:0032502 GO:0032787 GO:0043436 GO:0044237 GO:0044238 GO:0044281 GO:0044425 GO:0048468 GO:0048666 GO:0048699 GO:0048731 GO:0048856 GO:0048869 GO:0071704 GO:1901135 GO:1901136 GO:1901360 GO:1901575 GO:1901615 GO:1901657 GO:1901658
PFAM: PF12215.11

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0124650	XP_003590701.2	94.040	302	10	2	1	302	1	294	0.0	586

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0124650	sp\|Q9HCG7\|GBA2_HUMAN	36.111	252	134	6	62	309	106	334	3.28e-35	138

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0124650	G7I2L4	94.040	302	10	2	1	302	1	294	0.0	586

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0076710	Msa0124650	0.954609	2.010170e-112	-8.615850e-47
Msa0124650	Msa0179840	0.840856	6.756672e-58	-8.615850e-47
Msa0124650	Msa0227190	0.800178	1.627733e-48	-8.615850e-47
Msa0124650	Msa0796300	0.802153	6.421142e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0124650	MtrunA17_Chr1g0185871	94.040	302	10	2	1	302	1	294	0.0	586
Msa0124650	MtrunA17_Chr0c12g0493661	52.985	268	109	5	37	302	31	283	2.44e-90	288
Msa0124650	MtrunA17_Chr4g0076991	48.881	268	101	6	37	302	31	264	1.97e-79	256

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0124650	AT3G24180.1	78.378	296	53	2	7	302	1	285	3.89e-169	494
Msa0124650	AT3G24180.2	78.378	296	53	2	7	302	1	285	3.89e-169	494
Msa0124650	AT5G49900.3	52.060	267	114	3	36	302	23	275	3.07e-93	296
Msa0124650	AT5G49900.1	52.060	267	114	3	36	302	23	275	3.07e-93	296
Msa0124650	AT4G10060.1	46.909	275	132	4	28	302	8	268	1.53e-78	257
Msa0124650	AT4G10060.2	46.909	275	132	4	28	302	8	268	1.53e-78	257
Msa0124650	AT1G33700.4	49.813	267	128	4	36	302	13	273	1.56e-78	256
Msa0124650	AT1G33700.3	49.813	267	128	4	36	302	13	273	5.81e-78	256
Msa0124650	AT1G33700.1	49.813	267	128	4	36	302	13	273	5.81e-78	256
Msa0124650	AT1G33700.2	49.813	267	128	4	36	302	13	273	5.81e-78	256

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Find 266 sgRNAs with CRISPR-Local

Find 756 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGGAATGTGGACTAAAA+TGG	0.348084	1_3:+52497984	Msa0124650:three_prime_UTR
AAAATCGCGAGATATTGGAT+TGG	0.558031	1_3:-52490759	None:intergenic
AAAATGGTGGTCAAGGGCCG+GGG	0.531144	1_3:+52501726	Msa0124650:three_prime_UTR
AAAGTTAAAGGCGGCAGAAT+GGG	0.484438	1_3:+52500769	Msa0124650:three_prime_UTR
AAATCGCGAGATATTGGATT+GGG	0.518761	1_3:-52490758	None:intergenic
AAATGAGACTTACAGAGAAC+TGG	0.459619	1_3:-52492540	None:intergenic
AACAATGATACTTGCAGGAA+TGG	0.546051	1_3:+52500885	Msa0124650:three_prime_UTR
AACACCGAGTCAGCGTGGCG+TGG	0.632482	1_3:-52490691	None:intergenic
AACATAACATACCAACTGTA+AGG	0.499287	1_3:-52491088	None:intergenic
AACCGTCATATATAGCTATA+AGG	0.379301	1_3:-52495975	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTATGAAAATCGCGAGATAT+TGG	-	chr1_3:52490767-52490786	None:intergenic	30.0%
AAGATCGTTGAACAATATCT+AGG	+	chr1_3:52490921-52490940	Msa0124650:five_prime_UTR	30.0%
TATGAGAGATTTGGAAAGAA+TGG	+	chr1_3:52490999-52491018	Msa0124650:exon	30.0%
TTCATTGTAGAAAGAATTCG+TGG	+	chr1_3:52491037-52491056	Msa0124650:CDS	30.0%
AACATAACATACCAACTGTA+AGG	-	chr1_3:52491091-52491110	None:intergenic	30.0%
GAATATATTAGCCACCTATT+AGG	+	chr1_3:52491278-52491297	Msa0124650:intron	30.0%
AATGGTAATTACCTAATAGG+TGG	-	chr1_3:52491292-52491311	None:intergenic	30.0%
ACTAACTTAAGACACATTTG+AGG	+	chr1_3:52491455-52491474	Msa0124650:intron	30.0%
GAAGTAACTAACAAAAGAGA+AGG	+	chr1_3:52491481-52491500	Msa0124650:intron	30.0%
TGGAACAATTTAAATGTGTG+AGG	-	chr1_3:52491551-52491570	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_3	gene	52490575	52501964	52490575	ID=Msa0124650;Name=Msa0124650
chr1_3	mRNA	52490575	52501964	52490575	ID=Msa0124650-mRNA-1;Parent=Msa0124650;Name=Msa0124650-mRNA-1;_AED=0.13;_eAED=0.13;_QI=405\|0.89\|0.9\|0.95\|0.84\|0.75\|20\|2422\|311
chr1_3	exon	52490575	52490824	52490575	ID=Msa0124650-mRNA-1:exon:16125;Parent=Msa0124650-mRNA-1
chr1_3	exon	52490845	52491098	52490845	ID=Msa0124650-mRNA-1:exon:16126;Parent=Msa0124650-mRNA-1
chr1_3	exon	52491677	52491787	52491677	ID=Msa0124650-mRNA-1:exon:16127;Parent=Msa0124650-mRNA-1
chr1_3	exon	52491978	52492034	52491978	ID=Msa0124650-mRNA-1:exon:16128;Parent=Msa0124650-mRNA-1
chr1_3	exon	52492186	52492262	52492186	ID=Msa0124650-mRNA-1:exon:16129;Parent=Msa0124650-mRNA-1
chr1_3	exon	52492450	52492549	52492450	ID=Msa0124650-mRNA-1:exon:16130;Parent=Msa0124650-mRNA-1
chr1_3	exon	52493566	52493620	52493566	ID=Msa0124650-mRNA-1:exon:16131;Parent=Msa0124650-mRNA-1
chr1_3	exon	52493979	52494021	52493979	ID=Msa0124650-mRNA-1:exon:16132;Parent=Msa0124650-mRNA-1
chr1_3	exon	52494506	52494615	52494506	ID=Msa0124650-mRNA-1:exon:16133;Parent=Msa0124650-mRNA-1
chr1_3	exon	52494699	52494802	52494699	ID=Msa0124650-mRNA-1:exon:16134;Parent=Msa0124650-mRNA-1
chr1_3	exon	52495620	52495673	52495620	ID=Msa0124650-mRNA-1:exon:16135;Parent=Msa0124650-mRNA-1
chr1_3	exon	52495767	52495822	52495767	ID=Msa0124650-mRNA-1:exon:16136;Parent=Msa0124650-mRNA-1
chr1_3	exon	52495923	52495994	52495923	ID=Msa0124650-mRNA-1:exon:16137;Parent=Msa0124650-mRNA-1
chr1_3	exon	52497870	52498011	52497870	ID=Msa0124650-mRNA-1:exon:16138;Parent=Msa0124650-mRNA-1
chr1_3	exon	52498143	52498327	52498143	ID=Msa0124650-mRNA-1:exon:16139;Parent=Msa0124650-mRNA-1
chr1_3	exon	52498567	52498634	52498567	ID=Msa0124650-mRNA-1:exon:16140;Parent=Msa0124650-mRNA-1
chr1_3	exon	52498797	52498869	52498797	ID=Msa0124650-mRNA-1:exon:16141;Parent=Msa0124650-mRNA-1
chr1_3	exon	52499476	52499537	52499476	ID=Msa0124650-mRNA-1:exon:16142;Parent=Msa0124650-mRNA-1
chr1_3	exon	52499666	52500971	52499666	ID=Msa0124650-mRNA-1:exon:16143;Parent=Msa0124650-mRNA-1
chr1_3	exon	52501381	52501964	52501381	ID=Msa0124650-mRNA-1:exon:16144;Parent=Msa0124650-mRNA-1
chr1_3	five_prime_UTR	52490575	52490824	52490575	ID=Msa0124650-mRNA-1:five_prime_utr;Parent=Msa0124650-mRNA-1
chr1_3	five_prime_UTR	52490845	52490999	52490845	ID=Msa0124650-mRNA-1:five_prime_utr;Parent=Msa0124650-mRNA-1
chr1_3	CDS	52491000	52491098	52491000	ID=Msa0124650-mRNA-1:cds;Parent=Msa0124650-mRNA-1
chr1_3	CDS	52491677	52491787	52491677	ID=Msa0124650-mRNA-1:cds;Parent=Msa0124650-mRNA-1
chr1_3	CDS	52491978	52492034	52491978	ID=Msa0124650-mRNA-1:cds;Parent=Msa0124650-mRNA-1
chr1_3	CDS	52492186	52492262	52492186	ID=Msa0124650-mRNA-1:cds;Parent=Msa0124650-mRNA-1
chr1_3	CDS	52492450	52492549	52492450	ID=Msa0124650-mRNA-1:cds;Parent=Msa0124650-mRNA-1
chr1_3	CDS	52493566	52493620	52493566	ID=Msa0124650-mRNA-1:cds;Parent=Msa0124650-mRNA-1
chr1_3	CDS	52493979	52494021	52493979	ID=Msa0124650-mRNA-1:cds;Parent=Msa0124650-mRNA-1
chr1_3	CDS	52494506	52494615	52494506	ID=Msa0124650-mRNA-1:cds;Parent=Msa0124650-mRNA-1
chr1_3	CDS	52494699	52494802	52494699	ID=Msa0124650-mRNA-1:cds;Parent=Msa0124650-mRNA-1
chr1_3	CDS	52495620	52495673	52495620	ID=Msa0124650-mRNA-1:cds;Parent=Msa0124650-mRNA-1
chr1_3	CDS	52495767	52495822	52495767	ID=Msa0124650-mRNA-1:cds;Parent=Msa0124650-mRNA-1
chr1_3	CDS	52495923	52495992	52495923	ID=Msa0124650-mRNA-1:cds;Parent=Msa0124650-mRNA-1
chr1_3	three_prime_UTR	52495993	52495994	52495993	ID=Msa0124650-mRNA-1:three_prime_utr;Parent=Msa0124650-mRNA-1
chr1_3	three_prime_UTR	52497870	52498011	52497870	ID=Msa0124650-mRNA-1:three_prime_utr;Parent=Msa0124650-mRNA-1
chr1_3	three_prime_UTR	52498143	52498327	52498143	ID=Msa0124650-mRNA-1:three_prime_utr;Parent=Msa0124650-mRNA-1
chr1_3	three_prime_UTR	52498567	52498634	52498567	ID=Msa0124650-mRNA-1:three_prime_utr;Parent=Msa0124650-mRNA-1
chr1_3	three_prime_UTR	52498797	52498869	52498797	ID=Msa0124650-mRNA-1:three_prime_utr;Parent=Msa0124650-mRNA-1
chr1_3	three_prime_UTR	52499476	52499537	52499476	ID=Msa0124650-mRNA-1:three_prime_utr;Parent=Msa0124650-mRNA-1
chr1_3	three_prime_UTR	52499666	52500971	52499666	ID=Msa0124650-mRNA-1:three_prime_utr;Parent=Msa0124650-mRNA-1
chr1_3	three_prime_UTR	52501381	52501964	52501381	ID=Msa0124650-mRNA-1:three_prime_utr;Parent=Msa0124650-mRNA-1

Gene Sequence

>Msa0124650

ATGAGAGATTTGGAAAGAATGGTTAGCGGCAATTTATTTCATTGTAGAAAGAATTCGTGGCCTACTGAAGAGTATATCAATAAAACCACCTTACAGTTGTTTGATATTGATAATGCTGCTCCTCCTGAACAAGCTTGGAGAAGAAGATTAAACAGCCATGCCAATCTTCTTAAAGAATTTAGAGTAACTTTTATGGAAGCTATAAAGATGGTTCGATTAGGTATACGAATGTGGTCATATGTCAGGGAAGAGGCTTCTCATGGAAGGAAAGCACCTATAGATCCTTTCACTCGAGAAAGTTGCAAGCCATCTGCATCTCAAGGGGTTCCACTTGGAGGGATGGGGAGTGGCAGCATATCAAGAGGATTTAGAGGTGAATTCAGACAATGGCAAATCATTCCTGGTCTATGTGAACCATCACCTGTCATGGCCAACCAGTTCTCTATTTTTGTAAGTAGAGAGGGAGGAAACAAAAGTTTTGCATCAGTTTTGGCTCCCGATTCACCATTTTATGCCACTGTTACTGCTTCATGTCACTGCAAAATTAGCAGGAAAGCTGATGATCAGGGTATATCATCATGGGGATGGAATCTAAATGGCCAGCACTCAACATACCATGCTTTGTTTCCAAGAGCTTGGACTGTTTATGATGGTGAGCCAGACCCAGAACTAAAAATATCATGTCGGCAGATATCCCCTTTCATACCACATAATTACAGAGAAAGCAGTCTACCTGCTGCCGTTTTTGTCTATACGTTGGTAAACACTGGTAAAGAAAGAGCTAAAGTCAGCCTTTTATTTACTTGGGCGAATTCTATTGGTGGGAACTCTCACTTGTCAGGAGATCATGTGAATGAACCTTTCATAGCTGAAGATGGCGTCTCCGGCGTACTTCTATATCACAAGCAAGTTATCAAACCTTATAGCTATATATGA

Protein sequence

>Msa0124650

MRDLERMVSGNLFHCRKNSWPTEEYINKTTLQLFDIDNAAPPEQAWRRRLNSHANLLKEFRVTFMEAIKMVRLGIRMWSYVREEASHGRKAPIDPFTRESCKPSASQGVPLGGMGSGSISRGFRGEFRQWQIIPGLCEPSPVMANQFSIFVSREGGNKSFASVLAPDSPFYATVTASCHCKISRKADDQGISSWGWNLNGQHSTYHALFPRAWTVYDGEPDPELKISCRQISPFIPHNYRESSLPAAVFVYTLVNTGKERAKVSLLFTWANSIGGNSHLSGDHVNEPFIAEDGVSGVLLYHKQVIKPYSYI*