AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0125310

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0125310 MtrunA17_Chr1g0187241 79.330 358 38 4 4 325 116 473 0.0 545
Msa0125310 MtrunA17_Chr1g0166711 63.866 357 94 5 4 325 115 471 1.33e-150 436
Msa0125310 MtrunA17_Chr1g0166731 64.426 357 92 5 4 325 12 368 1.98e-149 428
Msa0125310 MtrunA17_Chr1g0166791 56.863 357 118 5 4 324 114 470 3.89e-135 396
Msa0125310 MtrunA17_Chr1g0166741 56.863 357 111 6 4 324 117 466 6.36e-131 385
Msa0125310 MtrunA17_Chr1g0166771 55.182 357 117 6 4 324 81 430 6.14e-127 374
Msa0125310 MtrunA17_Chr7g0261661 52.778 360 130 6 4 325 3 360 2.15e-123 363
Msa0125310 MtrunA17_Chr7g0261811 51.667 360 134 6 4 325 117 474 2.72e-116 348
Msa0125310 MtrunA17_Chr7g0261881 50.000 360 140 6 4 325 117 474 5.70e-116 347
Msa0125310 MtrunA17_Chr7g0261771 50.696 359 137 6 5 325 122 478 6.07e-114 342
Msa0125310 MtrunA17_Chr7g0261791 48.747 359 147 6 4 325 119 477 9.67e-108 326
Msa0125310 MtrunA17_Chr7g0261851 49.722 360 141 6 4 325 118 475 2.09e-102 312
Msa0125310 MtrunA17_Chr7g0261751 42.821 390 147 6 4 319 121 508 1.06e-98 300
Msa0125310 MtrunA17_Chr7g0261731 53.275 229 88 3 4 213 121 349 2.82e-79 245
Msa0125310 MtrunA17_Chr3g0114501 40.443 361 172 7 4 325 112 468 6.90e-71 230
Msa0125310 MtrunA17_Chr5g0422881 39.227 362 177 10 5 325 111 470 3.99e-68 223
Msa0125310 MtrunA17_Chr8g0375311 37.017 362 184 7 5 325 131 489 2.05e-65 216
Msa0125310 MtrunA17_Chr3g0104011 37.088 364 182 10 4 325 112 470 9.64e-65 214
Msa0125310 MtrunA17_Chr8g0375331 34.711 363 193 6 4 325 114 473 3.56e-64 212
Msa0125310 MtrunA17_Chr8g0375351 35.342 365 188 7 4 325 130 489 3.62e-64 213
Msa0125310 MtrunA17_Chr8g0375341 35.262 363 191 6 4 325 130 489 4.51e-63 209
Msa0125310 MtrunA17_Chr7g0255611 33.967 368 191 12 5 325 120 482 2.37e-62 208
Msa0125310 MtrunA17_Chr8g0375291 35.912 362 188 6 5 325 131 489 2.86e-62 207
Msa0125310 MtrunA17_Chr8g0375321 33.333 363 198 6 4 325 127 486 7.17e-62 206
Msa0125310 MtrunA17_Chr3g0142091 37.500 360 180 10 3 325 115 466 3.81e-60 202
Msa0125310 MtrunA17_Chr4g0056431 35.598 368 191 7 4 325 115 482 1.87e-59 200
Msa0125310 MtrunA17_Chr5g0399071 35.246 366 192 8 4 325 115 479 2.66e-59 200
Msa0125310 MtrunA17_Chr8g0375361 36.612 366 187 7 1 325 127 488 4.65e-59 199
Msa0125310 MtrunA17_Chr5g0399061 36.043 369 185 10 4 325 117 481 1.50e-58 197
Msa0125310 MtrunA17_Chr7g0240381 34.254 362 186 9 3 325 118 466 3.84e-58 196
Msa0125310 MtrunA17_Chr7g0241741 35.165 364 189 9 3 325 111 468 1.50e-57 194
Msa0125310 MtrunA17_Chr7g0255601 34.959 369 186 13 5 325 118 480 2.45e-57 195
Msa0125310 MtrunA17_Chr7g0255621 33.699 365 196 8 5 325 111 473 3.89e-57 194
Msa0125310 MtrunA17_Chr3g0133341 33.520 358 202 6 4 325 104 461 1.06e-56 192
Msa0125310 MtrunA17_Chr4g0005901 35.537 363 187 9 4 325 122 478 2.57e-56 191
Msa0125310 MtrunA17_Chr4g0008651 32.964 361 196 9 3 324 123 476 8.99e-56 190
Msa0125310 MtrunA17_Chr3g0112611 34.807 362 190 11 4 325 122 477 8.77e-55 188
Msa0125310 MtrunA17_Chr3g0142101 33.889 360 192 9 3 325 124 474 2.18e-54 186
Msa0125310 MtrunA17_Chr5g0400371 32.865 356 193 9 14 325 212 565 7.11e-54 187
Msa0125310 MtrunA17_Chr3g0082631 33.824 340 172 8 37 325 30 367 4.92e-52 178
Msa0125310 MtrunA17_Chr3g0112671 31.579 361 195 8 3 325 122 468 4.97e-52 180
Msa0125310 MtrunA17_Chr7g0217461 32.418 364 202 8 5 325 115 477 5.72e-52 180
Msa0125310 MtrunA17_Chr8g0353031 35.799 338 168 6 37 325 121 458 6.60e-52 180
Msa0125310 MtrunA17_Chr3g0077151 32.602 319 188 9 34 325 132 450 2.71e-50 175
Msa0125310 MtrunA17_Chr5g0415791 33.967 368 193 10 5 325 120 484 7.06e-50 175
Msa0125310 MtrunA17_Chr7g0214461 33.149 362 194 10 4 325 168 521 1.21e-49 174
Msa0125310 MtrunA17_Chr2g0284701 32.533 375 198 9 4 325 122 494 1.32e-49 174
Msa0125310 MtrunA17_Chr1g0213711 33.531 337 178 9 29 325 134 464 4.47e-49 172
Msa0125310 MtrunA17_Chr4g0014791 34.758 351 183 11 6 325 121 456 8.30e-49 171
Msa0125310 MtrunA17_Chr5g0439381 32.222 360 201 8 1 325 116 467 8.59e-49 172
Msa0125310 MtrunA17_Chr4g0014861 33.742 326 179 9 34 325 13 335 3.17e-48 167
Msa0125310 MtrunA17_Chr4g0054891 30.685 365 202 8 5 325 201 558 3.52e-48 171
Msa0125310 MtrunA17_Chr4g0014781 32.861 353 189 11 6 325 121 458 2.28e-47 167
Msa0125310 MtrunA17_Chr7g0218851 32.303 356 187 10 14 325 146 491 4.48e-47 167
Msa0125310 MtrunA17_Chr4g0014841 32.733 333 190 9 23 325 128 456 9.25e-47 166
Msa0125310 MtrunA17_Chr4g0063591 33.705 359 195 8 5 325 113 466 9.90e-46 163
Msa0125310 MtrunA17_Chr4g0014871 32.584 356 191 11 4 325 120 460 2.08e-45 162
Msa0125310 MtrunA17_Chr4g0014801 32.733 333 192 9 23 325 128 458 3.11e-45 161
Msa0125310 MtrunA17_Chr2g0276751 32.479 351 200 9 3 325 127 468 1.19e-44 160
Msa0125310 MtrunA17_Chr8g0362711 30.108 372 204 9 3 325 113 477 9.69e-44 157
Msa0125310 MtrunA17_Chr4g0060001 31.978 369 197 11 3 325 118 478 3.89e-41 151
Msa0125310 MtrunA17_Chr8g0362801 28.841 371 212 10 3 325 2 368 4.68e-41 149
Msa0125310 MtrunA17_Chr4g0059981 30.352 369 211 7 3 325 120 488 2.16e-40 149
Msa0125310 MtrunA17_Chr7g0214731 30.978 368 205 13 5 325 152 517 3.00e-40 149
Msa0125310 MtrunA17_Chr5g0415841 29.700 367 212 12 3 325 2 366 8.03e-40 145
Msa0125310 MtrunA17_Chr8g0379971 30.334 389 202 11 4 325 110 496 9.43e-40 147
Msa0125310 MtrunA17_Chr4g0011471 29.700 367 209 8 3 325 105 466 2.36e-39 145
Msa0125310 MtrunA17_Chr1g0147631 30.137 365 207 12 4 325 115 474 3.79e-39 145
Msa0125310 MtrunA17_Chr1g0157601 29.315 365 211 9 3 325 104 463 5.09e-38 142
Msa0125310 MtrunA17_Chr7g0271221 29.396 364 210 10 2 325 94 450 1.85e-37 140
Msa0125310 MtrunA17_Chr8g0390331 27.624 362 221 8 3 325 104 463 6.05e-37 139
Msa0125310 MtrunA17_Chr4g0059971 29.759 373 208 12 3 325 123 491 1.44e-36 138
Msa0125310 MtrunA17_Chr1g0193391 27.809 356 213 10 3 325 106 450 4.26e-36 137
Msa0125310 MtrunA17_Chr7g0261831 42.077 183 54 4 142 290 1 165 5.46e-36 129
Msa0125310 MtrunA17_Chr8g0362781 28.191 376 211 13 3 325 119 488 6.34e-36 136
Msa0125310 MtrunA17_Chr7g0240151 28.919 370 208 9 4 325 3 365 1.75e-35 134
Msa0125310 MtrunA17_Chr1g0157621 29.722 360 207 11 7 325 73 427 1.81e-35 135
Msa0125310 MtrunA17_Chr5g0415961 26.613 372 218 8 3 325 113 478 2.20e-35 135
Msa0125310 MtrunA17_Chr8g0379991 28.250 400 209 12 4 325 70 469 2.52e-35 134
Msa0125310 MtrunA17_Chr7g0235521 28.919 370 208 9 4 325 3 365 2.55e-35 133
Msa0125310 MtrunA17_Chr7g0240131 28.919 370 208 9 4 325 3 365 5.90e-35 132
Msa0125310 MtrunA17_Chr7g0240211 28.919 370 208 9 4 325 3 365 8.66e-35 132
Msa0125310 MtrunA17_Chr7g0240181 28.919 370 208 9 4 325 3 365 9.58e-35 132
Msa0125310 MtrunA17_Chr4g0069581 29.023 348 201 9 18 325 80 421 1.07e-34 132
Msa0125310 MtrunA17_Chr4g0059991 29.255 376 207 12 3 325 111 480 1.21e-34 133
Msa0125310 MtrunA17_Chr7g0240271 28.378 370 210 9 4 325 114 476 2.15e-34 132
Msa0125310 MtrunA17_Chr6g0451841 33.099 284 149 11 78 325 165 443 3.39e-34 131
Msa0125310 MtrunA17_Chr2g0331511 28.954 373 203 13 4 325 111 472 2.10e-33 129
Msa0125310 MtrunA17_Chr4g0011041 27.642 369 211 10 5 325 127 487 2.39e-33 129
Msa0125310 MtrunA17_Chr7g0240281 28.726 369 212 7 4 325 114 478 3.48e-33 129
Msa0125310 MtrunA17_Chr1g0157611 28.056 360 210 11 9 325 111 464 1.04e-32 127
Msa0125310 MtrunA17_Chr2g0331471 29.428 367 209 12 4 325 178 539 6.42e-32 125
Msa0125310 MtrunA17_Chr2g0331521 25.000 360 228 9 7 325 115 473 9.70e-32 124
Msa0125310 MtrunA17_Chr4g0003761 28.065 367 214 10 3 325 119 479 5.65e-31 122
Msa0125310 MtrunA17_Chr2g0326211 26.243 362 205 11 10 325 115 460 7.48e-31 122
Msa0125310 MtrunA17_Chr3g0142111 38.462 169 98 3 3 168 135 300 8.06e-31 119
Msa0125310 MtrunA17_Chr2g0325961 25.833 360 204 10 13 325 118 461 5.34e-29 116
Msa0125310 MtrunA17_Chr2g0331481 27.957 372 208 11 4 325 115 476 6.75e-29 117
Msa0125310 MtrunA17_Chr2g0325951 25.946 370 210 10 3 325 108 460 7.32e-29 116
Msa0125310 MtrunA17_Chr3g0112651 34.132 167 103 2 3 167 23 184 2.55e-28 108
Msa0125310 MtrunA17_Chr1g0177611 26.205 332 200 6 31 324 20 344 4.20e-28 113
Msa0125310 MtrunA17_Chr1g0177591 26.269 335 202 7 28 324 146 473 1.29e-27 112
Msa0125310 MtrunA17_Chr7g0261721 54.455 101 44 1 227 325 8 108 3.38e-26 103
Msa0125310 MtrunA17_Chr3g0112631 36.464 181 90 6 169 325 1 180 3.69e-25 102
Msa0125310 MtrunA17_Chr1g0177631 25.904 332 201 6 31 324 20 344 1.75e-24 103
Msa0125310 MtrunA17_Chr7g0234381 27.966 354 190 13 3 323 5 326 6.81e-24 101
Msa0125310 MtrunA17_Chr7g0234151 27.429 350 197 11 3 323 5 326 1.78e-23 99.8
Msa0125310 MtrunA17_Chr1g0147591 30.688 189 101 5 45 206 10 195 1.86e-22 93.2
Msa0125310 MtrunA17_Chr2g0331461 26.702 382 200 13 4 325 115 476 4.29e-22 97.1
Msa0125310 MtrunA17_Chr7g0234181 27.089 347 200 11 4 323 1 321 5.15e-22 95.9
Msa0125310 MtrunA17_Chr3g0142121 33.721 172 90 5 172 325 11 176 5.39e-19 85.5
Msa0125310 MtrunA17_Chr7g0261841 52.632 76 35 1 4 78 3 78 1.45e-17 76.6
Msa0125310 MtrunA17_Chr4g0060071 31.373 153 89 3 189 325 52 204 2.27e-16 78.2
Msa0125310 MtrunA17_Chr4g0060061 35.537 121 76 1 27 145 8 128 1.33e-15 72.4
Msa0125310 MtrunA17_Chr8g0382601 38.356 73 44 1 95 166 1 73 1.42e-12 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0125310 AT5G46050.2 57.939 359 115 5 3 325 2 360 2.24e-139 403
Msa0125310 AT5G46050.1 57.939 359 115 5 3 325 114 472 1.09e-137 402
Msa0125310 AT5G46040.1 56.425 358 120 6 4 325 115 472 1.90e-127 377
Msa0125310 AT2G40460.1 41.504 359 167 8 3 325 111 462 2.51e-78 250
Msa0125310 AT2G02040.1 37.569 362 182 7 5 325 130 488 3.05e-70 229
Msa0125310 AT1G62200.1 38.567 363 174 7 4 325 140 494 3.28e-70 229
Msa0125310 AT1G62200.2 35.714 392 174 7 4 325 140 523 1.97e-65 217
Msa0125310 AT1G62200.3 35.714 392 174 7 4 325 154 537 2.41e-65 217
Msa0125310 AT2G02020.1 38.235 340 173 7 5 325 131 452 3.58e-64 212
Msa0125310 AT3G54140.1 37.637 364 180 9 4 325 112 470 4.47e-64 212
Msa0125310 AT3G54140.2 37.637 364 180 9 4 325 112 470 4.47e-64 212
Msa0125310 AT5G01180.2 36.685 368 177 9 4 325 113 470 1.07e-60 203
Msa0125310 AT5G01180.3 36.685 368 177 9 4 325 113 470 1.07e-60 203
Msa0125310 AT5G01180.1 36.685 368 177 9 4 325 113 470 1.07e-60 203
Msa0125310 AT3G53960.1 35.616 365 185 9 3 325 124 480 1.19e-59 201
Msa0125310 AT3G54450.1 34.012 344 177 10 30 325 109 450 2.77e-55 189
Msa0125310 AT2G37900.2 32.597 362 192 9 3 325 46 394 1.81e-54 185
Msa0125310 AT1G68570.2 36.066 366 188 7 4 325 81 444 4.30e-54 186
Msa0125310 AT1G68570.1 36.339 366 187 8 4 325 111 474 6.97e-54 186
Msa0125310 AT2G37900.1 32.597 362 192 9 3 325 125 473 8.04e-54 185
Msa0125310 AT2G02020.2 35.435 333 171 7 5 325 131 431 3.56e-53 182
Msa0125310 AT1G12110.1 34.795 365 192 8 4 324 115 477 1.60e-51 179
Msa0125310 AT1G69870.1 32.967 364 199 9 5 325 140 501 5.02e-51 178
Msa0125310 AT2G26690.3 33.884 363 188 9 5 325 68 420 1.19e-49 173
Msa0125310 AT2G26690.2 33.884 363 188 9 5 325 80 432 1.22e-49 173
Msa0125310 AT2G26690.1 33.884 363 188 9 5 325 113 465 1.90e-49 174
Msa0125310 AT3G21670.1 32.687 361 198 8 3 325 116 469 5.73e-48 170
Msa0125310 AT1G32450.1 31.579 361 194 10 14 325 135 491 7.79e-48 170
Msa0125310 AT1G22570.2 32.822 326 179 8 37 325 111 433 4.46e-47 166
Msa0125310 AT1G22570.1 32.822 326 179 8 37 325 148 470 1.28e-46 166
Msa0125310 AT1G59740.1 32.500 360 201 8 3 325 128 482 1.44e-46 166
Msa0125310 AT1G33440.1 33.983 359 191 9 3 325 125 473 3.16e-46 165
Msa0125310 AT1G22540.1 31.805 349 198 11 9 325 119 459 3.50e-44 159
Msa0125310 AT1G22550.1 32.915 319 181 10 37 325 151 466 6.51e-44 158
Msa0125310 AT5G13400.1 32.249 369 199 13 5 325 153 518 1.94e-43 158
Msa0125310 AT3G45650.1 31.653 357 199 10 4 325 110 456 1.97e-43 157
Msa0125310 AT1G72140.1 32.011 353 194 10 4 325 122 459 6.91e-43 155
Msa0125310 AT1G27040.3 29.775 356 216 6 3 325 82 436 1.29e-42 154
Msa0125310 AT1G27040.2 29.775 356 216 6 3 325 110 464 2.17e-42 154
Msa0125310 AT1G27040.1 29.775 356 216 6 3 325 114 468 2.19e-42 154
Msa0125310 AT4G21680.1 31.063 367 208 9 3 325 115 480 2.30e-42 154
Msa0125310 AT1G72125.1 33.129 326 185 9 31 323 136 461 2.57e-42 154
Msa0125310 AT3G45650.2 31.534 352 196 10 4 320 110 451 1.64e-41 152
Msa0125310 AT1G72120.1 32.075 318 187 8 37 325 142 459 3.88e-41 150
Msa0125310 AT3G16180.1 28.962 366 216 9 3 324 110 475 1.82e-40 149
Msa0125310 AT1G69850.1 28.836 378 210 8 3 325 112 485 4.46e-40 148
Msa0125310 AT1G18880.1 30.400 375 206 12 2 324 101 472 1.06e-39 147
Msa0125310 AT5G19640.1 32.764 351 180 12 14 325 157 490 2.24e-39 146
Msa0125310 AT3G45720.1 30.601 366 193 12 3 325 106 453 1.72e-38 143
Msa0125310 AT3G45660.1 27.731 357 215 10 3 325 108 455 2.42e-37 140
Msa0125310 AT3G45660.3 27.731 357 215 10 3 325 108 455 2.42e-37 140
Msa0125310 AT3G45660.2 27.731 357 215 10 3 325 129 476 2.88e-37 140
Msa0125310 AT1G52190.1 28.261 368 218 11 3 324 108 475 3.72e-37 140
Msa0125310 AT3G45690.1 29.641 334 187 11 3 298 105 428 1.24e-36 138
Msa0125310 AT3G45710.1 30.328 366 194 12 4 325 109 457 1.41e-36 138
Msa0125310 AT1G72130.2 29.341 334 185 10 26 325 6 322 2.66e-36 135
Msa0125310 AT1G72130.3 29.341 334 185 10 26 325 6 322 2.66e-36 135
Msa0125310 AT1G72130.1 29.341 334 185 10 26 325 124 440 1.19e-35 135
Msa0125310 AT1G27080.2 29.670 364 202 9 5 325 61 413 6.29e-35 133
Msa0125310 AT1G27080.1 29.670 364 202 9 5 325 103 455 8.32e-35 133
Msa0125310 AT3G45700.1 29.722 360 197 12 4 325 104 445 1.19e-34 133
Msa0125310 AT5G62680.1 29.839 372 208 10 3 325 132 499 1.52e-34 133
Msa0125310 AT3G47960.1 29.947 374 205 14 3 325 148 515 3.62e-33 129
Msa0125310 AT5G28470.2 28.415 366 211 10 4 325 105 463 1.10e-32 127
Msa0125310 AT5G28470.1 28.022 364 213 9 4 325 105 461 9.51e-32 125
Msa0125310 AT3G45680.1 28.493 365 203 12 3 325 107 455 1.02e-31 124
Msa0125310 AT3G25260.1 30.857 350 185 13 3 323 108 429 1.20e-31 124
Msa0125310 AT5G14940.1 28.571 343 191 12 32 325 110 447 2.49e-29 118
Msa0125310 AT1G69860.1 27.424 361 216 9 3 325 105 457 1.39e-26 110
Msa0125310 AT3G01350.1 28.070 342 190 12 39 325 117 457 9.12e-26 107
Msa0125310 AT5G62730.2 27.523 327 195 9 41 325 106 432 5.77e-25 105
Msa0125310 AT5G62730.1 27.523 327 195 9 41 325 165 491 8.94e-25 105
Msa0125310 AT3G25280.1 27.507 349 198 12 3 323 108 429 8.94e-23 99.0
Msa0125310 AT2G38100.2 25.000 256 148 7 104 321 145 394 2.59e-16 79.7
Msa0125310 AT2G38100.1 25.391 256 147 7 104 321 161 410 3.24e-16 79.7
Msa0125310 AT2G38100.3 25.391 256 147 7 104 321 167 416 3.62e-16 79.3

Find 74 sgRNAs with CRISPR-Local

Find 224 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTTCCTCAGTTTATACTTA+TGG 0.141612 1_3:+53810197 Msa0125310:CDS
GGTAATCCCTCTTGGATTCT+TGG 0.231736 1_3:-53810030 None:intergenic
AGGCAATTTGTGTCTATAAA+AGG 0.238778 1_3:-53808991 None:intergenic
TAGTTGCCTCTTTGAGAAAA+TGG 0.247765 1_3:+53809048 Msa0125310:CDS
CTTCCTCAGTTTATACTTAT+GGG 0.256695 1_3:+53810198 Msa0125310:CDS
CATCCGTGACAGAAAATTAT+AGG 0.268828 1_3:+53810106 Msa0125310:CDS
CTAGAACAGTATTAGCAAAA+AGG 0.279901 1_3:-53808875 None:intergenic
TGGATGTTTAGCATCTTCTT+TGG 0.287426 1_3:+53808849 Msa0125310:intron
AATATCAATTATGATATTCT+TGG 0.288500 1_3:+53808959 Msa0125310:CDS
GCTAAGGATGATTCCAATCT+TGG 0.317325 1_3:+53809816 Msa0125310:CDS
TAGGGTGAAAATCATCAAAT+TGG 0.324030 1_3:-53808790 None:intergenic
CTTGTTCTCCACACAAATTA+TGG 0.328394 1_3:+53810077 Msa0125310:CDS
GGATGTTTAGCATCTTCTTT+GGG 0.346262 1_3:+53808850 Msa0125310:intron
AGAACTATATGAACTTGATA+TGG 0.372923 1_3:+53809097 Msa0125310:CDS
TCTATGACCGTTTCTTTGTA+AGG 0.389056 1_3:+53809991 Msa0125310:CDS
ACTTGAGCCACCATTGTACT+TGG 0.390318 1_3:-53809853 None:intergenic
TATAGTTCTTTAGTATCATC+AGG 0.412300 1_3:-53809083 None:intergenic
ACAAATGTAGCAACCAAGAT+TGG 0.425089 1_3:-53809829 None:intergenic
CAATCCCCATTCTTTGAAGA+AGG 0.427035 1_3:-53810052 None:intergenic
ATTACCCTTCTTCAAAGAAT+GGG 0.431335 1_3:+53810047 Msa0125310:CDS
TGAAGAAGGGTAATCCCTCT+TGG 0.431813 1_3:-53810038 None:intergenic
GAACATGGTTTAGTTGAAAG+TGG 0.432350 1_3:+53810144 Msa0125310:CDS
GCACTTTACACTCTAGCAAT+AGG 0.438872 1_3:+53808726 Msa0125310:CDS
ATGGCATTGTGGTGGCTTCA+AGG 0.445113 1_3:-53808643 None:intergenic
AGCACCAATGGTTGATATGT+TGG 0.454694 1_3:-53808765 None:intergenic
GATTACCCTTCTTCAAAGAA+TGG 0.458294 1_3:+53810046 Msa0125310:CDS
ACAAATGCAGCTAAACTTGC+TGG 0.466855 1_3:-53809939 None:intergenic
GATAATGTAGGGTGGACTCT+TGG 0.468721 1_3:+53808909 Msa0125310:CDS
TACACTCTAGCAATAGGAAC+AGG 0.472265 1_3:+53808732 Msa0125310:CDS
TCTTTAGTATCATCAGGAAC+AGG 0.472294 1_3:-53809077 None:intergenic
GGCAATTTGTGTCTATAAAA+GGG 0.473662 1_3:-53808990 None:intergenic
ATCAAATTGGTCAGCACCAA+TGG 0.477753 1_3:-53808777 None:intergenic
GACAAGGCAAGTGTCAAAAC+AGG 0.484313 1_3:+53809736 Msa0125310:CDS
GAGTCCACCCTACATTATCT+TGG 0.488116 1_3:-53808905 None:intergenic
TATAGACACAAATTGCCTGC+AGG 0.499408 1_3:+53808996 Msa0125310:CDS
GCATAATCCTTACAAAGAAA+CGG 0.503299 1_3:-53809998 None:intergenic
GTTTATATCCAAGATAATGT+AGG 0.508581 1_3:+53808897 Msa0125310:CDS
GGATATGCTTTACCAACTCT+AGG 0.512162 1_3:+53808930 Msa0125310:CDS
TCAATTATGATATTCTTGGC+AGG 0.514039 1_3:+53808963 Msa0125310:CDS
TACTTTCACTAGAATGGCTA+GGG 0.514636 1_3:+53809022 Msa0125310:CDS
GATATTGCTAATCCTAGAGT+TGG 0.517407 1_3:-53808942 None:intergenic
AGGGAGTACTTTCACTAGAA+TGG 0.522005 1_3:+53809016 Msa0125310:CDS
GTACTTTCACTAGAATGGCT+AGG 0.525257 1_3:+53809021 Msa0125310:CDS
CTTAGAGTGGCAAAAGAACA+TGG 0.538649 1_3:+53810129 Msa0125310:CDS
CTTCTTCAAAGAATGGGGAT+TGG 0.539956 1_3:+53810053 Msa0125310:CDS
AAAACCAACATATCAACCAT+TGG 0.542463 1_3:+53808761 Msa0125310:CDS
GTGACACTGCTAGTGTAAGC+AGG 0.550795 1_3:-53808614 None:intergenic
TTTATATCCAAGATAATGTA+GGG 0.553143 1_3:+53808898 Msa0125310:CDS
CACAGCTAATTGTAATGTAG+AGG 0.555926 1_3:-53808694 None:intergenic
AAGAGACAAAACAAATGCTA+AGG 0.557028 1_3:+53809800 Msa0125310:CDS
ATAGACACAAATTGCCTGCA+GGG 0.564786 1_3:+53808997 Msa0125310:CDS
CTAGTGAAAGTACTCCCTGC+AGG 0.565881 1_3:-53809011 None:intergenic
CATTGTGGTGGCTTCAAGGT+AGG 0.568165 1_3:-53808639 None:intergenic
ACAACACTTGACAGACACAT+TGG 0.573195 1_3:+53809901 Msa0125310:intron
TACATTTGTTCCAAGTACAA+TGG 0.576794 1_3:+53809843 Msa0125310:CDS
AATCCCCATTCTTTGAAGAA+GGG 0.582506 1_3:-53810051 None:intergenic
TTACCCTTCTTCAAAGAATG+GGG 0.585217 1_3:+53810048 Msa0125310:CDS
AGCAAAATGAAAATACTCAA+AGG 0.587653 1_3:-53810177 None:intergenic
ATTATTTAGGTTCCTAGACA+AGG 0.594422 1_3:+53809720 Msa0125310:intron
AAACAGGCACAACTAGTCCA+TGG 0.601903 1_3:+53809752 Msa0125310:CDS
GTAACTGTGCATAACATCCA+TGG 0.611443 1_3:-53809769 None:intergenic
TGCAACTACCATAATTTGTG+TGG 0.614854 1_3:-53810085 None:intergenic
TCTAATTCATGGCATTGTGG+TGG 0.621164 1_3:-53808651 None:intergenic
AATGCAGCTAAACTTGCTGG+TGG 0.624594 1_3:-53809936 None:intergenic
ACTCTAGCAATAGGAACAGG+TGG 0.624774 1_3:+53808735 Msa0125310:CDS
AGAAAATTATAGGCTTAGAG+TGG 0.632729 1_3:+53810116 Msa0125310:CDS
ACATCTAATTCATGGCATTG+TGG 0.635027 1_3:-53808654 None:intergenic
ATTTGTTCCAAGTACAATGG+TGG 0.636724 1_3:+53809846 Msa0125310:CDS
CATGGTTTAGTTGAAAGTGG+AGG 0.662978 1_3:+53810147 Msa0125310:CDS
GAGCACTACACAAACAAGCA+AGG 0.664656 1_3:-53809970 None:intergenic
GATTAACCAAGAATCCAAGA+GGG 0.678167 1_3:+53810024 Msa0125310:CDS
AGATTAACCAAGAATCCAAG+AGG 0.693948 1_3:+53810023 Msa0125310:CDS
GTTCCCATAAGTATAAACTG+AGG 0.698344 1_3:-53810201 None:intergenic
ATATCCAAGATAATGTAGGG+TGG 0.707851 1_3:+53808901 Msa0125310:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAATTTTCTGAAATATTTA+TGG + chr1_3:53809303-53809322 Msa0125310:intron 10.0%
!!! ATAATTTTCTGAAATATTTA+TGG + chr1_3:53809303-53809322 Msa0125310:intron 10.0%
!!! TAAACTTTCATTTTTTCAAT+TGG + chr1_3:53808826-53808845 Msa0125310:CDS 15.0%
!! AATATCAATTATGATATTCT+TGG + chr1_3:53808959-53808978 Msa0125310:CDS 15.0%
!!! TAAACTTTCATTTTTTCAAT+TGG + chr1_3:53808826-53808845 Msa0125310:CDS 15.0%
!! AATATCAATTATGATATTCT+TGG + chr1_3:53808959-53808978 Msa0125310:CDS 15.0%
!!! ATTTTGTTACATCTAATTCA+TGG - chr1_3:53808665-53808684 None:intergenic 20.0%
!!! GTTTATGTGATTTTTCTTTA+GGG - chr1_3:53808811-53808830 None:intergenic 20.0%
!!! AGTTTATGTGATTTTTCTTT+AGG - chr1_3:53808812-53808831 None:intergenic 20.0%
!! TTTATATCCAAGATAATGTA+GGG + chr1_3:53808898-53808917 Msa0125310:CDS 20.0%
!!! TTTCGCTATAATTTTTTAAG+GGG - chr1_3:53809610-53809629 None:intergenic 20.0%
!!! CTTTCGCTATAATTTTTTAA+GGG - chr1_3:53809611-53809630 None:intergenic 20.0%
!!! ACTTTCGCTATAATTTTTTA+AGG - chr1_3:53809612-53809631 None:intergenic 20.0%
!!! TGTTTTATTTTCTTAACACA+TGG - chr1_3:53809689-53809708 None:intergenic 20.0%
!!! ATTTTGTTACATCTAATTCA+TGG - chr1_3:53808665-53808684 None:intergenic 20.0%
!!! GTTTATGTGATTTTTCTTTA+GGG - chr1_3:53808811-53808830 None:intergenic 20.0%
!!! AGTTTATGTGATTTTTCTTT+AGG - chr1_3:53808812-53808831 None:intergenic 20.0%
!! TTTATATCCAAGATAATGTA+GGG + chr1_3:53808898-53808917 Msa0125310:CDS 20.0%
!!! TTTCGCTATAATTTTTTAAG+GGG - chr1_3:53809610-53809629 None:intergenic 20.0%
!!! CTTTCGCTATAATTTTTTAA+GGG - chr1_3:53809611-53809630 None:intergenic 20.0%
!!! ACTTTCGCTATAATTTTTTA+AGG - chr1_3:53809612-53809631 None:intergenic 20.0%
!!! TGTTTTATTTTCTTAACACA+TGG - chr1_3:53809689-53809708 None:intergenic 20.0%
!! TTACAATTAGCTGTGTTTTA+TGG + chr1_3:53808702-53808721 Msa0125310:CDS 25.0%
!!! ACTTTCATTTTTTCAATTGG+TGG + chr1_3:53808829-53808848 Msa0125310:CDS 25.0%
! TAGAACAGTATTAGCAAAAA+GGG - chr1_3:53808877-53808896 None:intergenic 25.0%
! GTTTATATCCAAGATAATGT+AGG + chr1_3:53808897-53808916 Msa0125310:CDS 25.0%
! TATAGTTCTTTAGTATCATC+AGG - chr1_3:53809086-53809105 None:intergenic 25.0%
! AGAACTATATGAACTTGATA+TGG + chr1_3:53809097-53809116 Msa0125310:CDS 25.0%
! AAATGTATATACCTCAATGT+TGG - chr1_3:53809170-53809189 None:intergenic 25.0%
! GTGTTAAAGTTAATTAAGCT+AGG - chr1_3:53809201-53809220 None:intergenic 25.0%
! GCTTAATTAACTTTAACACA+CGG + chr1_3:53809202-53809221 Msa0125310:intron 25.0%
!!! TGTAGTTTTACTTAAAGAAC+TGG + chr1_3:53809390-53809409 Msa0125310:intron 25.0%
!!! TAATCCTTAAAAGTTTGTTG+CGG - chr1_3:53809431-53809450 None:intergenic 25.0%
! TGATTGATTATGTTCAATAG+TGG + chr1_3:53809470-53809489 Msa0125310:intron 25.0%
! AACTTCATTAACCACAAATT+TGG + chr1_3:53809536-53809555 Msa0125310:intron 25.0%
! AGCAAAATGAAAATACTCAA+AGG - chr1_3:53810180-53810199 None:intergenic 25.0%
!! TTACAATTAGCTGTGTTTTA+TGG + chr1_3:53808702-53808721 Msa0125310:CDS 25.0%
!!! ACTTTCATTTTTTCAATTGG+TGG + chr1_3:53808829-53808848 Msa0125310:CDS 25.0%
! TAGAACAGTATTAGCAAAAA+GGG - chr1_3:53808877-53808896 None:intergenic 25.0%
! GTTTATATCCAAGATAATGT+AGG + chr1_3:53808897-53808916 Msa0125310:CDS 25.0%
! TATAGTTCTTTAGTATCATC+AGG - chr1_3:53809086-53809105 None:intergenic 25.0%
! AGAACTATATGAACTTGATA+TGG + chr1_3:53809097-53809116 Msa0125310:CDS 25.0%
! AAATGTATATACCTCAATGT+TGG - chr1_3:53809170-53809189 None:intergenic 25.0%
! GTGTTAAAGTTAATTAAGCT+AGG - chr1_3:53809201-53809220 None:intergenic 25.0%
! GCTTAATTAACTTTAACACA+CGG + chr1_3:53809202-53809221 Msa0125310:intron 25.0%
!!! TGTAGTTTTACTTAAAGAAC+TGG + chr1_3:53809390-53809409 Msa0125310:intron 25.0%
!!! TAATCCTTAAAAGTTTGTTG+CGG - chr1_3:53809431-53809450 None:intergenic 25.0%
! TGATTGATTATGTTCAATAG+TGG + chr1_3:53809470-53809489 Msa0125310:intron 25.0%
! AACTTCATTAACCACAAATT+TGG + chr1_3:53809536-53809555 Msa0125310:intron 25.0%
! AGCAAAATGAAAATACTCAA+AGG - chr1_3:53810180-53810199 None:intergenic 25.0%
AAAACCAACATATCAACCAT+TGG + chr1_3:53808761-53808780 Msa0125310:CDS 30.0%
TAGGGTGAAAATCATCAAAT+TGG - chr1_3:53808793-53808812 None:intergenic 30.0%
CTAGAACAGTATTAGCAAAA+AGG - chr1_3:53808878-53808897 None:intergenic 30.0%
TCAATTATGATATTCTTGGC+AGG + chr1_3:53808963-53808982 Msa0125310:CDS 30.0%
GGCAATTTGTGTCTATAAAA+GGG - chr1_3:53808993-53809012 None:intergenic 30.0%
AGGCAATTTGTGTCTATAAA+AGG - chr1_3:53808994-53809013 None:intergenic 30.0%
! TACTTTCCATTTTCTCAAAG+AGG - chr1_3:53809057-53809076 None:intergenic 30.0%
! TGAAATATTTATGGTGTCGA+CGG + chr1_3:53809312-53809331 Msa0125310:intron 30.0%
TAAGTACTAACACAAAACAC+CGG - chr1_3:53809354-53809373 None:intergenic 30.0%
! ATTATTTAGGTTCCTAGACA+AGG + chr1_3:53809720-53809739 Msa0125310:intron 30.0%
AAGAGACAAAACAAATGCTA+AGG + chr1_3:53809800-53809819 Msa0125310:CDS 30.0%
TACATTTGTTCCAAGTACAA+TGG + chr1_3:53809843-53809862 Msa0125310:CDS 30.0%
!!! TGTTCCTTGTTTTACAAAGA+GGG - chr1_3:53809884-53809903 None:intergenic 30.0%
!!! TTGTTCCTTGTTTTACAAAG+AGG - chr1_3:53809885-53809904 None:intergenic 30.0%
GCATAATCCTTACAAAGAAA+CGG - chr1_3:53810001-53810020 None:intergenic 30.0%
ATTACCCTTCTTCAAAGAAT+GGG + chr1_3:53810047-53810066 Msa0125310:CDS 30.0%
! AAGCCTATAATTTTCTGTCA+CGG - chr1_3:53810112-53810131 None:intergenic 30.0%
AGAAAATTATAGGCTTAGAG+TGG + chr1_3:53810116-53810135 Msa0125310:CDS 30.0%
CTTCCTCAGTTTATACTTAT+GGG + chr1_3:53810198-53810217 Msa0125310:CDS 30.0%
AAAACCAACATATCAACCAT+TGG + chr1_3:53808761-53808780 Msa0125310:CDS 30.0%
TAGGGTGAAAATCATCAAAT+TGG - chr1_3:53808793-53808812 None:intergenic 30.0%
CTAGAACAGTATTAGCAAAA+AGG - chr1_3:53808878-53808897 None:intergenic 30.0%
TCAATTATGATATTCTTGGC+AGG + chr1_3:53808963-53808982 Msa0125310:CDS 30.0%
GGCAATTTGTGTCTATAAAA+GGG - chr1_3:53808993-53809012 None:intergenic 30.0%
AGGCAATTTGTGTCTATAAA+AGG - chr1_3:53808994-53809013 None:intergenic 30.0%
! TACTTTCCATTTTCTCAAAG+AGG - chr1_3:53809057-53809076 None:intergenic 30.0%
! TGAAATATTTATGGTGTCGA+CGG + chr1_3:53809312-53809331 Msa0125310:intron 30.0%
TAAGTACTAACACAAAACAC+CGG - chr1_3:53809354-53809373 None:intergenic 30.0%
! ATTATTTAGGTTCCTAGACA+AGG + chr1_3:53809720-53809739 Msa0125310:intron 30.0%
AAGAGACAAAACAAATGCTA+AGG + chr1_3:53809800-53809819 Msa0125310:CDS 30.0%
TACATTTGTTCCAAGTACAA+TGG + chr1_3:53809843-53809862 Msa0125310:CDS 30.0%
!!! TGTTCCTTGTTTTACAAAGA+GGG - chr1_3:53809884-53809903 None:intergenic 30.0%
!!! TTGTTCCTTGTTTTACAAAG+AGG - chr1_3:53809885-53809904 None:intergenic 30.0%
GCATAATCCTTACAAAGAAA+CGG - chr1_3:53810001-53810020 None:intergenic 30.0%
ATTACCCTTCTTCAAAGAAT+GGG + chr1_3:53810047-53810066 Msa0125310:CDS 30.0%
! AAGCCTATAATTTTCTGTCA+CGG - chr1_3:53810112-53810131 None:intergenic 30.0%
AGAAAATTATAGGCTTAGAG+TGG + chr1_3:53810116-53810135 Msa0125310:CDS 30.0%
CTTCCTCAGTTTATACTTAT+GGG + chr1_3:53810198-53810217 Msa0125310:CDS 30.0%
ACATCTAATTCATGGCATTG+TGG - chr1_3:53808657-53808676 None:intergenic 35.0%
CACAGCTAATTGTAATGTAG+AGG - chr1_3:53808697-53808716 None:intergenic 35.0%
TGGATGTTTAGCATCTTCTT+TGG + chr1_3:53808849-53808868 Msa0125310:intron 35.0%
GGATGTTTAGCATCTTCTTT+GGG + chr1_3:53808850-53808869 Msa0125310:intron 35.0%
ATATCCAAGATAATGTAGGG+TGG + chr1_3:53808901-53808920 Msa0125310:CDS 35.0%
GATATTGCTAATCCTAGAGT+TGG - chr1_3:53808945-53808964 None:intergenic 35.0%
TACTTTCACTAGAATGGCTA+GGG + chr1_3:53809022-53809041 Msa0125310:CDS 35.0%
! TAGTTGCCTCTTTGAGAAAA+TGG + chr1_3:53809048-53809067 Msa0125310:CDS 35.0%
TCTTTAGTATCATCAGGAAC+AGG - chr1_3:53809080-53809099 None:intergenic 35.0%
ATGGTAGAGTATGCTAAGAA+AGG + chr1_3:53809116-53809135 Msa0125310:intron 35.0%
TGGTAGAGTATGCTAAGAAA+GGG + chr1_3:53809117-53809136 Msa0125310:intron 35.0%
TATATACCTCAATGTTGGAG+TGG - chr1_3:53809165-53809184 None:intergenic 35.0%
TGTCTAACACATGTCAATGT+TGG + chr1_3:53809245-53809264 Msa0125310:intron 35.0%
! CTTTCCGCAACAAACTTTTA+AGG + chr1_3:53809424-53809443 Msa0125310:intron 35.0%
GTTAAACACGTCCAAATTTG+TGG - chr1_3:53809550-53809569 None:intergenic 35.0%
!!! AATTTTTTAAGGGGTCCATG+TGG - chr1_3:53809601-53809620 None:intergenic 35.0%
ACAAATGTAGCAACCAAGAT+TGG - chr1_3:53809832-53809851 None:intergenic 35.0%
ATTTGTTCCAAGTACAATGG+TGG + chr1_3:53809846-53809865 Msa0125310:CDS 35.0%
AACACCCTCTTTGTAAAACA+AGG + chr1_3:53809877-53809896 Msa0125310:intron 35.0%
TCTATGACCGTTTCTTTGTA+AGG + chr1_3:53809991-53810010 Msa0125310:CDS 35.0%
AGATTAACCAAGAATCCAAG+AGG + chr1_3:53810023-53810042 Msa0125310:CDS 35.0%
GATTAACCAAGAATCCAAGA+GGG + chr1_3:53810024-53810043 Msa0125310:CDS 35.0%
GATTACCCTTCTTCAAAGAA+TGG + chr1_3:53810046-53810065 Msa0125310:CDS 35.0%
TTACCCTTCTTCAAAGAATG+GGG + chr1_3:53810048-53810067 Msa0125310:CDS 35.0%
! AATCCCCATTCTTTGAAGAA+GGG - chr1_3:53810054-53810073 None:intergenic 35.0%
CTTGTTCTCCACACAAATTA+TGG + chr1_3:53810077-53810096 Msa0125310:CDS 35.0%
! TGCAACTACCATAATTTGTG+TGG - chr1_3:53810088-53810107 None:intergenic 35.0%
CATCCGTGACAGAAAATTAT+AGG + chr1_3:53810106-53810125 Msa0125310:CDS 35.0%
GAACATGGTTTAGTTGAAAG+TGG + chr1_3:53810144-53810163 Msa0125310:CDS 35.0%
GCTTCCTCAGTTTATACTTA+TGG + chr1_3:53810197-53810216 Msa0125310:CDS 35.0%
GTTCCCATAAGTATAAACTG+AGG - chr1_3:53810204-53810223 None:intergenic 35.0%
ACATCTAATTCATGGCATTG+TGG - chr1_3:53808657-53808676 None:intergenic 35.0%
CACAGCTAATTGTAATGTAG+AGG - chr1_3:53808697-53808716 None:intergenic 35.0%
TGGATGTTTAGCATCTTCTT+TGG + chr1_3:53808849-53808868 Msa0125310:intron 35.0%
GGATGTTTAGCATCTTCTTT+GGG + chr1_3:53808850-53808869 Msa0125310:intron 35.0%
ATATCCAAGATAATGTAGGG+TGG + chr1_3:53808901-53808920 Msa0125310:CDS 35.0%
GATATTGCTAATCCTAGAGT+TGG - chr1_3:53808945-53808964 None:intergenic 35.0%
TACTTTCACTAGAATGGCTA+GGG + chr1_3:53809022-53809041 Msa0125310:CDS 35.0%
! TAGTTGCCTCTTTGAGAAAA+TGG + chr1_3:53809048-53809067 Msa0125310:CDS 35.0%
TCTTTAGTATCATCAGGAAC+AGG - chr1_3:53809080-53809099 None:intergenic 35.0%
ATGGTAGAGTATGCTAAGAA+AGG + chr1_3:53809116-53809135 Msa0125310:intron 35.0%
TGGTAGAGTATGCTAAGAAA+GGG + chr1_3:53809117-53809136 Msa0125310:intron 35.0%
TATATACCTCAATGTTGGAG+TGG - chr1_3:53809165-53809184 None:intergenic 35.0%
TGTCTAACACATGTCAATGT+TGG + chr1_3:53809245-53809264 Msa0125310:intron 35.0%
! CTTTCCGCAACAAACTTTTA+AGG + chr1_3:53809424-53809443 Msa0125310:intron 35.0%
GTTAAACACGTCCAAATTTG+TGG - chr1_3:53809550-53809569 None:intergenic 35.0%
!!! AATTTTTTAAGGGGTCCATG+TGG - chr1_3:53809601-53809620 None:intergenic 35.0%
ACAAATGTAGCAACCAAGAT+TGG - chr1_3:53809832-53809851 None:intergenic 35.0%
ATTTGTTCCAAGTACAATGG+TGG + chr1_3:53809846-53809865 Msa0125310:CDS 35.0%
AACACCCTCTTTGTAAAACA+AGG + chr1_3:53809877-53809896 Msa0125310:intron 35.0%
TCTATGACCGTTTCTTTGTA+AGG + chr1_3:53809991-53810010 Msa0125310:CDS 35.0%
AGATTAACCAAGAATCCAAG+AGG + chr1_3:53810023-53810042 Msa0125310:CDS 35.0%
GATTAACCAAGAATCCAAGA+GGG + chr1_3:53810024-53810043 Msa0125310:CDS 35.0%
GATTACCCTTCTTCAAAGAA+TGG + chr1_3:53810046-53810065 Msa0125310:CDS 35.0%
TTACCCTTCTTCAAAGAATG+GGG + chr1_3:53810048-53810067 Msa0125310:CDS 35.0%
! AATCCCCATTCTTTGAAGAA+GGG - chr1_3:53810054-53810073 None:intergenic 35.0%
CTTGTTCTCCACACAAATTA+TGG + chr1_3:53810077-53810096 Msa0125310:CDS 35.0%
! TGCAACTACCATAATTTGTG+TGG - chr1_3:53810088-53810107 None:intergenic 35.0%
CATCCGTGACAGAAAATTAT+AGG + chr1_3:53810106-53810125 Msa0125310:CDS 35.0%
GAACATGGTTTAGTTGAAAG+TGG + chr1_3:53810144-53810163 Msa0125310:CDS 35.0%
GCTTCCTCAGTTTATACTTA+TGG + chr1_3:53810197-53810216 Msa0125310:CDS 35.0%
GTTCCCATAAGTATAAACTG+AGG - chr1_3:53810204-53810223 None:intergenic 35.0%
TCTAATTCATGGCATTGTGG+TGG - chr1_3:53808654-53808673 None:intergenic 40.0%
GCACTTTACACTCTAGCAAT+AGG + chr1_3:53808726-53808745 Msa0125310:CDS 40.0%
TACACTCTAGCAATAGGAAC+AGG + chr1_3:53808732-53808751 Msa0125310:CDS 40.0%
! AGCACCAATGGTTGATATGT+TGG - chr1_3:53808768-53808787 None:intergenic 40.0%
ATCAAATTGGTCAGCACCAA+TGG - chr1_3:53808780-53808799 None:intergenic 40.0%
GGATATGCTTTACCAACTCT+AGG + chr1_3:53808930-53808949 Msa0125310:CDS 40.0%
TATAGACACAAATTGCCTGC+AGG + chr1_3:53808996-53809015 Msa0125310:CDS 40.0%
ATAGACACAAATTGCCTGCA+GGG + chr1_3:53808997-53809016 Msa0125310:CDS 40.0%
!! AGGGAGTACTTTCACTAGAA+TGG + chr1_3:53809016-53809035 Msa0125310:CDS 40.0%
GTACTTTCACTAGAATGGCT+AGG + chr1_3:53809021-53809040 Msa0125310:CDS 40.0%
TTGATTCCACTCCAACATTG+AGG + chr1_3:53809156-53809175 Msa0125310:intron 40.0%
CGTTCAAATCTACAACCACA+TGG + chr1_3:53809583-53809602 Msa0125310:intron 40.0%
! TTTTGACACTTGCCTTGTCT+AGG - chr1_3:53809735-53809754 None:intergenic 40.0%
GTAACTGTGCATAACATCCA+TGG - chr1_3:53809772-53809791 None:intergenic 40.0%
GCTAAGGATGATTCCAATCT+TGG + chr1_3:53809816-53809835 Msa0125310:CDS 40.0%
ACAACACTTGACAGACACAT+TGG + chr1_3:53809901-53809920 Msa0125310:intron 40.0%
ACAAATGCAGCTAAACTTGC+TGG - chr1_3:53809942-53809961 None:intergenic 40.0%
! CAATCCCCATTCTTTGAAGA+AGG - chr1_3:53810055-53810074 None:intergenic 40.0%
CTTCTTCAAAGAATGGGGAT+TGG + chr1_3:53810053-53810072 Msa0125310:CDS 40.0%
CTTAGAGTGGCAAAAGAACA+TGG + chr1_3:53810129-53810148 Msa0125310:CDS 40.0%
CATGGTTTAGTTGAAAGTGG+AGG + chr1_3:53810147-53810166 Msa0125310:CDS 40.0%
TCTAATTCATGGCATTGTGG+TGG - chr1_3:53808654-53808673 None:intergenic 40.0%
GCACTTTACACTCTAGCAAT+AGG + chr1_3:53808726-53808745 Msa0125310:CDS 40.0%
TACACTCTAGCAATAGGAAC+AGG + chr1_3:53808732-53808751 Msa0125310:CDS 40.0%
! AGCACCAATGGTTGATATGT+TGG - chr1_3:53808768-53808787 None:intergenic 40.0%
ATCAAATTGGTCAGCACCAA+TGG - chr1_3:53808780-53808799 None:intergenic 40.0%
GGATATGCTTTACCAACTCT+AGG + chr1_3:53808930-53808949 Msa0125310:CDS 40.0%
TATAGACACAAATTGCCTGC+AGG + chr1_3:53808996-53809015 Msa0125310:CDS 40.0%
ATAGACACAAATTGCCTGCA+GGG + chr1_3:53808997-53809016 Msa0125310:CDS 40.0%
!! AGGGAGTACTTTCACTAGAA+TGG + chr1_3:53809016-53809035 Msa0125310:CDS 40.0%
GTACTTTCACTAGAATGGCT+AGG + chr1_3:53809021-53809040 Msa0125310:CDS 40.0%
TTGATTCCACTCCAACATTG+AGG + chr1_3:53809156-53809175 Msa0125310:intron 40.0%
CGTTCAAATCTACAACCACA+TGG + chr1_3:53809583-53809602 Msa0125310:intron 40.0%
! TTTTGACACTTGCCTTGTCT+AGG - chr1_3:53809735-53809754 None:intergenic 40.0%
GTAACTGTGCATAACATCCA+TGG - chr1_3:53809772-53809791 None:intergenic 40.0%
GCTAAGGATGATTCCAATCT+TGG + chr1_3:53809816-53809835 Msa0125310:CDS 40.0%
ACAACACTTGACAGACACAT+TGG + chr1_3:53809901-53809920 Msa0125310:intron 40.0%
ACAAATGCAGCTAAACTTGC+TGG - chr1_3:53809942-53809961 None:intergenic 40.0%
! CAATCCCCATTCTTTGAAGA+AGG - chr1_3:53810055-53810074 None:intergenic 40.0%
CTTCTTCAAAGAATGGGGAT+TGG + chr1_3:53810053-53810072 Msa0125310:CDS 40.0%
CTTAGAGTGGCAAAAGAACA+TGG + chr1_3:53810129-53810148 Msa0125310:CDS 40.0%
CATGGTTTAGTTGAAAGTGG+AGG + chr1_3:53810147-53810166 Msa0125310:CDS 40.0%
ACTCTAGCAATAGGAACAGG+TGG + chr1_3:53808735-53808754 Msa0125310:CDS 45.0%
GAGTCCACCCTACATTATCT+TGG - chr1_3:53808908-53808927 None:intergenic 45.0%
! GATAATGTAGGGTGGACTCT+TGG + chr1_3:53808909-53808928 Msa0125310:CDS 45.0%
!! CGGCATTTTAGTGTAGTGTC+CGG + chr1_3:53809332-53809351 Msa0125310:intron 45.0%
GACAAGGCAAGTGTCAAAAC+AGG + chr1_3:53809736-53809755 Msa0125310:CDS 45.0%
AAACAGGCACAACTAGTCCA+TGG + chr1_3:53809752-53809771 Msa0125310:CDS 45.0%
ACTTGAGCCACCATTGTACT+TGG - chr1_3:53809856-53809875 None:intergenic 45.0%
!! AATGCAGCTAAACTTGCTGG+TGG - chr1_3:53809939-53809958 None:intergenic 45.0%
GAGCACTACACAAACAAGCA+AGG - chr1_3:53809973-53809992 None:intergenic 45.0%
GGTAATCCCTCTTGGATTCT+TGG - chr1_3:53810033-53810052 None:intergenic 45.0%
TGAAGAAGGGTAATCCCTCT+TGG - chr1_3:53810041-53810060 None:intergenic 45.0%
ACTCTAGCAATAGGAACAGG+TGG + chr1_3:53808735-53808754 Msa0125310:CDS 45.0%
GAGTCCACCCTACATTATCT+TGG - chr1_3:53808908-53808927 None:intergenic 45.0%
! GATAATGTAGGGTGGACTCT+TGG + chr1_3:53808909-53808928 Msa0125310:CDS 45.0%
!! CGGCATTTTAGTGTAGTGTC+CGG + chr1_3:53809332-53809351 Msa0125310:intron 45.0%
GACAAGGCAAGTGTCAAAAC+AGG + chr1_3:53809736-53809755 Msa0125310:CDS 45.0%
AAACAGGCACAACTAGTCCA+TGG + chr1_3:53809752-53809771 Msa0125310:CDS 45.0%
ACTTGAGCCACCATTGTACT+TGG - chr1_3:53809856-53809875 None:intergenic 45.0%
!! AATGCAGCTAAACTTGCTGG+TGG - chr1_3:53809939-53809958 None:intergenic 45.0%
GAGCACTACACAAACAAGCA+AGG - chr1_3:53809973-53809992 None:intergenic 45.0%
GGTAATCCCTCTTGGATTCT+TGG - chr1_3:53810033-53810052 None:intergenic 45.0%
TGAAGAAGGGTAATCCCTCT+TGG - chr1_3:53810041-53810060 None:intergenic 45.0%
!! CATATATTAATTTATTATTT+AGG + chr1_3:53809707-53809726 Msa0125310:intron 5.0%
!! CATATATTAATTTATTATTT+AGG + chr1_3:53809707-53809726 Msa0125310:intron 5.0%
GTGACACTGCTAGTGTAAGC+AGG - chr1_3:53808617-53808636 None:intergenic 50.0%
CATTGTGGTGGCTTCAAGGT+AGG - chr1_3:53808642-53808661 None:intergenic 50.0%
ATGGCATTGTGGTGGCTTCA+AGG - chr1_3:53808646-53808665 None:intergenic 50.0%
!! CTAGTGAAAGTACTCCCTGC+AGG - chr1_3:53809014-53809033 None:intergenic 50.0%
GTGACACTGCTAGTGTAAGC+AGG - chr1_3:53808617-53808636 None:intergenic 50.0%
CATTGTGGTGGCTTCAAGGT+AGG - chr1_3:53808642-53808661 None:intergenic 50.0%
ATGGCATTGTGGTGGCTTCA+AGG - chr1_3:53808646-53808665 None:intergenic 50.0%
!! CTAGTGAAAGTACTCCCTGC+AGG - chr1_3:53809014-53809033 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 53808606 53810236 53808606 ID=Msa0125310;Name=Msa0125310
chr1_3 mRNA 53808606 53810236 53808606 ID=Msa0125310-mRNA-1;Parent=Msa0125310;Name=Msa0125310-mRNA-1;_AED=0.23;_eAED=0.24;_QI=0|0|0|1|0|0|5|0|325
chr1_3 exon 53808606 53808853 53808606 ID=Msa0125310-mRNA-1:exon:16528;Parent=Msa0125310-mRNA-1
chr1_3 exon 53808860 53809123 53808860 ID=Msa0125310-mRNA-1:exon:16529;Parent=Msa0125310-mRNA-1
chr1_3 exon 53809729 53809873 53809729 ID=Msa0125310-mRNA-1:exon:16530;Parent=Msa0125310-mRNA-1
chr1_3 exon 53809915 53810098 53809915 ID=Msa0125310-mRNA-1:exon:16531;Parent=Msa0125310-mRNA-1
chr1_3 exon 53810103 53810236 53810103 ID=Msa0125310-mRNA-1:exon:16532;Parent=Msa0125310-mRNA-1
chr1_3 CDS 53808606 53808853 53808606 ID=Msa0125310-mRNA-1:cds;Parent=Msa0125310-mRNA-1
chr1_3 CDS 53808860 53809123 53808860 ID=Msa0125310-mRNA-1:cds;Parent=Msa0125310-mRNA-1
chr1_3 CDS 53809729 53809873 53809729 ID=Msa0125310-mRNA-1:cds;Parent=Msa0125310-mRNA-1
chr1_3 CDS 53809915 53810098 53809915 ID=Msa0125310-mRNA-1:cds;Parent=Msa0125310-mRNA-1
chr1_3 CDS 53810103 53810236 53810103 ID=Msa0125310-mRNA-1:cds;Parent=Msa0125310-mRNA-1
Gene Sequence

>Msa0125310

GTATGTCTCCTGCTTACACTAGCAGTGTCACTTCCTACCTTGAAGCCACCACAATGCCATGAATTAGATGTAACAAAATGCAAAAAAGCCTCTACATTACAATTAGCTGTGTTTTATGGTGCACTTTACACTCTAGCAATAGGAACAGGTGGAACAAAACCAACATATCAACCATTGGTGCTGACCAATTTGATGATTTTCACCCTAAAGAAAAATCACATAAACTTTCATTTTTTCAATTGGTGGATCATCTTCTTTGGGACCCTTTTTGCTAATACTGTTCTAGTTTATATCCAAGATAATGTAGGGTGGACTCTTGGATATGCTTTACCAACTCTAGGATTAGCAATATCAATTATGATATTCTTGGCAGGAACACCCTTTTATAGACACAAATTGCCTGCAGGGAGTACTTTCACTAGAATGGCTAGGGTTATAGTTGCCTCTTTGAGAAAATGGAAAGTACCTGTTCCTGATGATACTAAAGAACTATATGAACTTGATATGGTAGAGTTCCTAGACAAGGCAAGTGTCAAAACAGGCACAACTAGTCCATGGATGTTATGCACAGTTACACATGTAGAAGAGACAAAACAAATGCTAAGGATGATTCCAATCTTGGTTGCTACATTTGTTCCAAGTACAATGGTGGCTCAAACACATTGGAAGCTTCAAAATCCACCAGCAAGTTTAGCTGCATTTGTCACATTGTCCTTGCTTGTTTGTGTAGTGCTCTATGACCGTTTCTTTGTAAGGATTATGCAGAGATTAACCAAGAATCCAAGAGGGATTACCCTTCTTCAAAGAATGGGGATTGGTCTTGTTCTCCACACAAATTATGTTGCATCCGTGACAGAAAATTATAGGCTTAGAGTGGCAAAAGAACATGGTTTAGTTGAAAGTGGAGGACAAGTTCCTTTGAGTATTTTCATTTTGCTTCCTCAGTTTATACTTATGGGAACAGCTGATGCATTT
Protein sequence

>Msa0125310

VCLLLTLAVSLPTLKPPQCHELDVTKCKKASTLQLAVFYGALYTLAIGTGGTKPTYQPLVLTNLMIFTLKKNHINFHFFNWWIIFFGTLFANTVLVYIQDNVGWTLGYALPTLGLAISIMIFLAGTPFYRHKLPAGSTFTRMARVIVASLRKWKVPVPDDTKELYELDMVEFLDKASVKTGTTSPWMLCTVTHVEETKQMLRMIPILVATFVPSTMVAQTHWKLQNPPASLAAFVTLSLLVCVVLYDRFFVRIMQRLTKNPRGITLLQRMGIGLVLHTNYVASVTENYRLRVAKEHGLVESGGQVPLSIFILLPQFILMGTADAF