AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0128440

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0128440 MtrunA17_Chr1g0194891 98.558 208 3 0 1 208 1 208 7.54e-152 419
Msa0128440 MtrunA17_Chr4g0014721 29.268 123 87 0 32 154 228 350 4.45e-15 73.2
Msa0128440 MtrunA17_Chr4g0014741 29.268 123 87 0 32 154 228 350 4.50e-15 73.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0128440 AT3G62580.1 74.648 213 49 1 1 208 1 213 1.20e-115 328
Msa0128440 AT1G72100.1 28.723 188 121 4 25 206 296 476 9.96e-14 69.7
Msa0128440 AT1G22600.1 28.342 187 114 4 41 208 198 383 1.01e-11 63.5

Find 85 sgRNAs with CRISPR-Local

Find 224 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCGCTTTCGGCGCCGCTCTA+TGG 0.164177 1_3:-58166882 Msa0128440:CDS
TTATTCATCTTTGCAAGCTT+TGG 0.242253 1_3:+58164293 None:intergenic
GATTCCAGCGCTCGTGGGTT+TGG 0.328551 1_3:+58167171 None:intergenic
CTTGCCAACATCATGTCATT+TGG 0.339373 1_3:-58164239 Msa0128440:CDS
CGCTTTCGGCGCCGCTCTAT+GGG 0.344664 1_3:-58166881 Msa0128440:CDS
AATAGTATTGGAGAGGAAGT+TGG 0.347347 1_3:-58164350 Msa0128440:CDS
AAGAAGTTTGGTATGATTCA+TGG 0.351606 1_3:-58164272 Msa0128440:CDS
CTCGGCTTCTCCATCGCTTT+CGG 0.353954 1_3:-58166895 Msa0128440:CDS
AATTTGCCTAGGCATCAATT+TGG 0.358606 1_3:-58166725 Msa0128440:CDS
GCTGTCACTGTGGCTTCCTT+TGG 0.364294 1_3:-58166647 Msa0128440:CDS
TTTCTCAGTGTGCTATTCTC+TGG 0.370714 1_3:-58163938 Msa0128440:three_prime_UTR
TGACGGTGGTGGTGGTGTTC+TGG 0.374589 1_3:+58167044 None:intergenic
TAATATGGGGTAGCTGTATT+AGG 0.376418 1_3:-58167237 None:intergenic
AACCCACGAGCGCTGGAATC+TGG 0.377154 1_3:-58167168 Msa0128440:five_prime_UTR
GCAAAGATGAATAAGAAGTT+TGG 0.383264 1_3:-58164284 Msa0128440:CDS
ATTATGCTATAAATTATATA+AGG 0.396138 1_3:-58163974 Msa0128440:three_prime_UTR
TGCAATACACTCTTGGTACT+TGG 0.405780 1_3:-58164210 Msa0128440:CDS
TGGTGTAAAGACGAACAAAT+TGG 0.408776 1_3:+58166541 None:intergenic
TCTAGAAACCGAAGATCATT+TGG 0.420817 1_3:-58167199 Msa0128440:five_prime_UTR
ATAGTATTGGAGAGGAAGTT+GGG 0.421614 1_3:-58164349 Msa0128440:CDS
CGAGGAGATGAGCGAGTTTG+AGG 0.436447 1_3:+58166914 None:intergenic
TGGATTGACCAAATGATCTT+CGG 0.439017 1_3:+58167191 None:intergenic
TACAAGTATCTCTACTAGAA+TGG 0.440265 1_3:+58164152 None:intergenic
GTGGAGAGAGAGAATAGTAT+TGG 0.444526 1_3:-58164362 Msa0128440:CDS
TAATGAGGTAGTTGAATTGA+AGG 0.451077 1_3:+58167081 None:intergenic
GAGATACTTGTATGTGCAAT+TGG 0.451414 1_3:-58164141 Msa0128440:three_prime_UTR
CAATCCAAACCCACGAGCGC+TGG 0.468537 1_3:-58167175 Msa0128440:five_prime_UTR
ACAAGTATCTCTACTAGAAT+GGG 0.473721 1_3:+58164153 None:intergenic
AGAAGTTTGGTATGATTCAT+GGG 0.477765 1_3:-58164271 Msa0128440:CDS
GAAAATACCTCAATTGTCAT+TGG 0.481213 1_3:+58166521 None:intergenic
ATGAGCGAGTTTGAGGTAAG+TGG 0.481460 1_3:+58166921 None:intergenic
GAAGCCTTGCAATACACTCT+TGG 0.483767 1_3:-58164217 Msa0128440:CDS
GCTCTATGGGTCACCTTCAT+CGG 0.492086 1_3:-58166868 Msa0128440:CDS
ACGAGCGCTGGAATCTGGTT+TGG 0.492118 1_3:-58167163 Msa0128440:five_prime_UTR
ATTGATTTACCAAGAGTATC+CGG 0.495468 1_3:-58164089 Msa0128440:three_prime_UTR
ACCACCACCACCGTCATCAA+TGG 0.497424 1_3:-58167037 Msa0128440:exon
AGATTACCAAATTGATGCCT+AGG 0.499514 1_3:+58166719 None:intergenic
TGGTGTTCTGGTTCTGAGAA+AGG 0.511223 1_3:+58167056 None:intergenic
AATGAGGTAGTTGAATTGAA+GGG 0.512893 1_3:+58167082 None:intergenic
CCACCACCACCGTCATCAAT+GGG 0.515355 1_3:-58167036 Msa0128440:exon
CTCAAACTCGCTCATCTCCT+CGG 0.520112 1_3:-58166913 Msa0128440:CDS
GATGAAACAAAGGCATAAAG+TGG 0.520254 1_3:-58164381 Msa0128440:CDS
CCCATTGATGACGGTGGTGG+TGG 0.521011 1_3:+58167036 None:intergenic
CTTTACACCAATGACAATTG+AGG 0.521289 1_3:-58166528 Msa0128440:intron
ATACACTCTTGGTACTTGGC+AGG 0.533564 1_3:-58164206 Msa0128440:CDS
AGCAAGACACCGGATACTCT+TGG 0.534506 1_3:+58164080 None:intergenic
GAGAGAGAATAGTATTGGAG+AGG 0.541841 1_3:-58164357 Msa0128440:CDS
TTGTGTTTAGGAATTTGCCT+AGG 0.542157 1_3:-58166736 Msa0128440:intron
TGGTGAGAAAATTACTCCGA+TGG 0.542306 1_3:+58166975 None:intergenic
TTAGCATGTGGGGAATAAAA+CGG 0.543297 1_3:-58163884 Msa0128440:three_prime_UTR
AAACCAGATTCCAGCGCTCG+TGG 0.544546 1_3:+58167165 None:intergenic
TAGAGCGGCGCCGAAAGCGA+TGG 0.548120 1_3:+58166885 None:intergenic
CAAATGACATGATGTTGGCA+AGG 0.567231 1_3:+58164240 None:intergenic
CATGATGATACCGCCGATGA+AGG 0.570088 1_3:+58166855 None:intergenic
AAGTTGGTATCTTTCACTAG+TGG 0.574244 1_3:+58166598 None:intergenic
CGAAAGCGATGGAGAAGCCG+AGG 0.581248 1_3:+58166896 None:intergenic
ATGAGGTAGTTGAATTGAAG+GGG 0.581274 1_3:+58167083 None:intergenic
TTTGAGGTAAGTGGAAAGAG+TGG 0.582513 1_3:+58166930 None:intergenic
ATGTACCTTTCTGTAGTCAA+AGG 0.582953 1_3:-58164006 Msa0128440:three_prime_UTR
GAGGTAAGTGGAAAGAGTGG+TGG 0.583033 1_3:+58166933 None:intergenic
TTTGACTACAGAAAGGTACA+TGG 0.584102 1_3:+58164008 None:intergenic
ACTAGTGAAAGATACCAACT+TGG 0.589922 1_3:-58166596 Msa0128440:CDS
CCTTTGGTTATCTTCATCCG+TGG 0.593206 1_3:-58166631 Msa0128440:CDS
GCTTCCAAATGACATGATGT+TGG 0.594501 1_3:+58164235 None:intergenic
CAGAGATCAAGAAACCAAGT+TGG 0.596357 1_3:+58166582 None:intergenic
AAATGACATGATGTTGGCAA+GGG 0.609102 1_3:+58164241 None:intergenic
AACCAGATTCCAGCGCTCGT+GGG 0.615154 1_3:+58167166 None:intergenic
TGATATAGATGATGAAACAA+AGG 0.622671 1_3:-58164391 Msa0128440:intron
AGTATCTCTACTAGAATGGG+AGG 0.623701 1_3:+58164156 None:intergenic
CCACGGATGAAGATAACCAA+AGG 0.626168 1_3:+58166631 None:intergenic
CTACCCATTGATGACGGTGG+TGG 0.630995 1_3:+58167033 None:intergenic
ATGTTTGCACAGCAAGACAC+CGG 0.631554 1_3:+58164070 None:intergenic
GCGGCTCTACCCATTGATGA+CGG 0.643113 1_3:+58167027 None:intergenic
CTATGGGTCACCTTCATCGG+CGG 0.646660 1_3:-58166865 Msa0128440:CDS
GTATACCTTTGACTACAGAA+AGG 0.656498 1_3:+58164001 None:intergenic
AACCGCGGTTGTTATTAGAG+CGG 0.674556 1_3:+58167008 None:intergenic
GATGAAGGTGACCCATAGAG+CGG 0.683702 1_3:+58166870 None:intergenic
GTTCTGAGAAAGGAGTAATG+AGG 0.684686 1_3:+58167066 None:intergenic
AGTACCAAGAGTGTATTGCA+AGG 0.685013 1_3:+58164213 None:intergenic
TGTTTGCATTGCTGTCACTG+TGG 0.686509 1_3:-58166657 Msa0128440:CDS
TGTACTATCAAAGCATCACA+CGG 0.689750 1_3:-58164113 Msa0128440:three_prime_UTR
GCTCTACCCATTGATGACGG+TGG 0.693373 1_3:+58167030 None:intergenic
GTATTGGAGAGGAAGTTGGG+TGG 0.710494 1_3:-58164346 Msa0128440:CDS
AGCCGCTCTAATAACAACCG+CGG 0.732310 1_3:-58167010 Msa0128440:CDS
GATGGCTAAGAAAACAACCG+CGG 0.788667 1_3:+58166993 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGATTAAATTAACATAATTA+GGG + chr1_3:58164310-58164329 None:intergenic 10.0%
!! ATTATGCTATAAATTATATA+AGG - chr1_3:58167120-58167139 Msa0128440:five_prime_UTR 10.0%
!! GATTAAATTAACATAATTAG+GGG + chr1_3:58164309-58164328 None:intergenic 15.0%
!! CTGATTAAATTAACATAATT+AGG + chr1_3:58164311-58164330 None:intergenic 15.0%
!!! TATTTTATTCAAGTAAACTA+AGG + chr1_3:58167060-58167079 None:intergenic 15.0%
!!! TTCTTTTCTTTGTCTTTATA+AGG - chr1_3:58164615-58164634 Msa0128440:intron 20.0%
!!! ATGTTTATAGAATTTTGACA+TGG + chr1_3:58165179-58165198 None:intergenic 20.0%
!! AAATGAAAGAATCAAGAAAA+GGG + chr1_3:58165382-58165401 None:intergenic 20.0%
!! AAAATGAAAGAATCAAGAAA+AGG + chr1_3:58165383-58165402 None:intergenic 20.0%
!! ATTACTTTACCAAATATGTA+TGG - chr1_3:58165424-58165443 Msa0128440:intron 20.0%
!! AATATTTCACCATACATATT+TGG + chr1_3:58165436-58165455 None:intergenic 20.0%
!! TGTAAAAGATGGTAAAAAAT+AGG - chr1_3:58166081-58166100 Msa0128440:intron 20.0%
!! AACTATTAGAAAAGATTTAG+AGG - chr1_3:58166223-58166242 Msa0128440:intron 20.0%
!! AATATAGCATAACATGTATA+GGG + chr1_3:58166379-58166398 None:intergenic 20.0%
!! TAATATAGCATAACATGTAT+AGG + chr1_3:58166380-58166399 None:intergenic 20.0%
!! TTGTAACATGATTTATGTTA+GGG + chr1_3:58166494-58166513 None:intergenic 20.0%
!! TCTAATAAAAAATTAGCATG+TGG - chr1_3:58167198-58167217 Msa0128440:five_prime_UTR 20.0%
!! CTAATAAAAAATTAGCATGT+GGG - chr1_3:58167199-58167218 Msa0128440:five_prime_UTR 20.0%
!! TAATAAAAAATTAGCATGTG+GGG - chr1_3:58167200-58167219 Msa0128440:five_prime_UTR 20.0%
! GAAATTGATTAATTGATGGA+TGG + chr1_3:58164287-58164306 None:intergenic 25.0%
! AGAAGAATAATGAGAAAAGA+CGG - chr1_3:58164716-58164735 Msa0128440:intron 25.0%
!!! ACCTATGCATAAGTTTTATT+AGG - chr1_3:58164834-58164853 Msa0128440:intron 25.0%
!!! CCTATGCATAAGTTTTATTA+GGG - chr1_3:58164835-58164854 Msa0128440:intron 25.0%
! GATTATAGATATCTCTACAA+GGG + chr1_3:58165055-58165074 None:intergenic 25.0%
!! CTTGTATTGCATAAGTTTTT+AGG - chr1_3:58165501-58165520 Msa0128440:intron 25.0%
! AAAAAATATACCGATGGTTA+AGG + chr1_3:58165538-58165557 None:intergenic 25.0%
! CAATTCAAAAAATATACCGA+TGG + chr1_3:58165544-58165563 None:intergenic 25.0%
! AACAATAACAACAAATGTTC+AGG - chr1_3:58165687-58165706 Msa0128440:intron 25.0%
! CAAAAAACAAATCGAACAAA+AGG + chr1_3:58165747-58165766 None:intergenic 25.0%
!!! CTTTTGTTCGATTTGTTTTT+TGG - chr1_3:58165745-58165764 Msa0128440:intron 25.0%
! TACTTTCATATTGCATGTAT+AGG + chr1_3:58165852-58165871 None:intergenic 25.0%
! AATTTGCCTTTCAATTTAAG+CGG + chr1_3:58165875-58165894 None:intergenic 25.0%
!! TTTACCATCTTTTACACTAT+AGG + chr1_3:58166077-58166096 None:intergenic 25.0%
! TACAATACTCATAACATTGT+CGG - chr1_3:58166403-58166422 Msa0128440:intron 25.0%
! GTTGTAACATGATTTATGTT+AGG + chr1_3:58166495-58166514 None:intergenic 25.0%
! TAAAGTTTGAAAACTTACGT+TGG + chr1_3:58166563-58166582 None:intergenic 25.0%
! TCTATATCAGACAAACAAAA+CGG + chr1_3:58166692-58166711 None:intergenic 25.0%
! TGATATAGATGATGAAACAA+AGG - chr1_3:58166703-58166722 Msa0128440:CDS 25.0%
AATGAGGTAGTTGAATTGAA+GGG + chr1_3:58164015-58164034 None:intergenic 30.0%
TAATGAGGTAGTTGAATTGA+AGG + chr1_3:58164016-58164035 None:intergenic 30.0%
! ATTTTCTCACCAGAAACTTT+CGG - chr1_3:58164130-58164149 Msa0128440:three_prime_UTR 30.0%
AGGGGAAATTGATTAATTGA+TGG + chr1_3:58164291-58164310 None:intergenic 30.0%
!! GTTTCCAGCTTATTTTTCAA+TGG - chr1_3:58164410-58164429 Msa0128440:intron 30.0%
GAAAATACCTCAATTGTCAT+TGG + chr1_3:58164576-58164595 None:intergenic 30.0%
GAAGAATAATGAGAAAAGAC+GGG - chr1_3:58164717-58164736 Msa0128440:intron 30.0%
GTAAGTTATTCATCCAAACA+TGG + chr1_3:58164808-58164827 None:intergenic 30.0%
CCCTAATAAAACTTATGCAT+AGG + chr1_3:58164838-58164857 None:intergenic 30.0%
CGATTATAGATATCTCTACA+AGG + chr1_3:58165056-58165075 None:intergenic 30.0%
!!! GATAAGCTTTTTCAAACACT+GGG - chr1_3:58165075-58165094 Msa0128440:intron 30.0%
! GTAAGCTCAAATAAGCTTTT+TGG - chr1_3:58165113-58165132 Msa0128440:intron 30.0%
TCTTGCAATTGAATCAACTA+AGG - chr1_3:58165210-58165229 Msa0128440:intron 30.0%
CTAACAGGAATCAACATTTA+AGG + chr1_3:58165241-58165260 None:intergenic 30.0%
TGATTCCTGTTAGTAATTAG+AGG - chr1_3:58165248-58165267 Msa0128440:intron 30.0%
GATTCCTGTTAGTAATTAGA+GGG - chr1_3:58165249-58165268 Msa0128440:intron 30.0%
! CTAAATGTTCTCTTTTGTGT+TGG - chr1_3:58165304-58165323 Msa0128440:intron 30.0%
! GTAATGTTTTCCTTAACCAT+CGG - chr1_3:58165525-58165544 Msa0128440:intron 30.0%
ATTTCCTGTTCTATCTTCTT+TGG - chr1_3:58165615-58165634 Msa0128440:intron 30.0%
AAATCAGTCAACACAAAAGT+AGG - chr1_3:58165777-58165796 Msa0128440:intron 30.0%
AATCAGTCAACACAAAAGTA+GGG - chr1_3:58165778-58165797 Msa0128440:intron 30.0%
TATTGCATGTATAGGCAAAA+TGG + chr1_3:58165844-58165863 None:intergenic 30.0%
!! AAAGTACCGCTTAAATTGAA+AGG - chr1_3:58165866-58165885 Msa0128440:intron 30.0%
TGTTGTATGGTATGAAGTAT+TGG - chr1_3:58165920-58165939 Msa0128440:intron 30.0%
! GTTGTATGGTATGAAGTATT+GGG - chr1_3:58165921-58165940 Msa0128440:intron 30.0%
AGATGGTAAAAAATAGGCTT+AGG - chr1_3:58166087-58166106 Msa0128440:intron 30.0%
GATGAGAGTCAAATTATAAG+AGG - chr1_3:58166170-58166189 Msa0128440:intron 30.0%
AGTCAAATTATAAGAGGTAG+AGG - chr1_3:58166176-58166195 Msa0128440:intron 30.0%
AGATTTAGAGGTTAATGAGT+TGG - chr1_3:58166235-58166254 Msa0128440:intron 30.0%
! TAATGAGTTGGATCCAAATA+TGG - chr1_3:58166247-58166266 Msa0128440:intron 30.0%
ATAACATGTATAGGGCAAAA+TGG + chr1_3:58166371-58166390 None:intergenic 30.0%
TCATCTTGCAAACATAAGTT+TGG + chr1_3:58166532-58166551 None:intergenic 30.0%
TTAACCAGAGATATGATTCA+AGG + chr1_3:58166597-58166616 None:intergenic 30.0%
TTATTCATCTTTGCAAGCTT+TGG + chr1_3:58166804-58166823 None:intergenic 30.0%
GCAAAGATGAATAAGAAGTT+TGG - chr1_3:58166810-58166829 Msa0128440:intron 30.0%
AAGAAGTTTGGTATGATTCA+TGG - chr1_3:58166822-58166841 Msa0128440:intron 30.0%
AGAAGTTTGGTATGATTCAT+GGG - chr1_3:58166823-58166842 Msa0128440:intron 30.0%
ACAAGTATCTCTACTAGAAT+GGG + chr1_3:58166944-58166963 None:intergenic 30.0%
TACAAGTATCTCTACTAGAA+TGG + chr1_3:58166945-58166964 None:intergenic 30.0%
!! ATTGATTTACCAAGAGTATC+CGG - chr1_3:58167005-58167024 Msa0128440:CDS 30.0%
TCTAGAAACCGAAGATCATT+TGG - chr1_3:58163895-58163914 Msa0128440:three_prime_UTR 35.0%
TGGATTGACCAAATGATCTT+CGG + chr1_3:58163906-58163925 None:intergenic 35.0%
ATGAGGTAGTTGAATTGAAG+GGG + chr1_3:58164014-58164033 None:intergenic 35.0%
!! GATTTTGATCCGAAAGTTTC+TGG + chr1_3:58164142-58164161 None:intergenic 35.0%
ACGCTGAATTTCTTGTGTTT+AGG - chr1_3:58164346-58164365 Msa0128440:CDS 35.0%
TTGTGTTTAGGAATTTGCCT+AGG - chr1_3:58164358-58164377 Msa0128440:CDS 35.0%
AATTTGCCTAGGCATCAATT+TGG - chr1_3:58164369-58164388 Msa0128440:CDS 35.0%
AGATTACCAAATTGATGCCT+AGG + chr1_3:58164378-58164397 None:intergenic 35.0%
CCAACCATTGAAAAATAAGC+TGG + chr1_3:58164417-58164436 None:intergenic 35.0%
!! CCAGCTTATTTTTCAATGGT+TGG - chr1_3:58164414-58164433 Msa0128440:intron 35.0%
!! AAGTTGGTATCTTTCACTAG+TGG + chr1_3:58164499-58164518 None:intergenic 35.0%
ACTAGTGAAAGATACCAACT+TGG - chr1_3:58164498-58164517 Msa0128440:intron 35.0%
TGGTGTAAAGACGAACAAAT+TGG + chr1_3:58164556-58164575 None:intergenic 35.0%
CTTTACACCAATGACAATTG+AGG - chr1_3:58164566-58164585 Msa0128440:intron 35.0%
TAGCTTAACACCTTATAGAG+AGG + chr1_3:58164748-58164767 None:intergenic 35.0%
CTTTAAGCTGGAAACTACTT+AGG - chr1_3:58164782-58164801 Msa0128440:intron 35.0%
AAGATATCCTGGAGATCTTA+TGG - chr1_3:58164914-58164933 Msa0128440:intron 35.0%
CTTATTTCCATAAGATCTCC+AGG + chr1_3:58164924-58164943 None:intergenic 35.0%
CTACAAGGGTCTATTTGTAT+TGG + chr1_3:58165041-58165060 None:intergenic 35.0%
!!! CGATAAGCTTTTTCAAACAC+TGG - chr1_3:58165074-58165093 Msa0128440:intron 35.0%
! GGATGGAATTGAGTTTTTGA+AGG - chr1_3:58165270-58165289 Msa0128440:intron 35.0%
AGAAGATAGAACAGGAAATG+AGG + chr1_3:58165614-58165633 None:intergenic 35.0%
TGAACCAAAGAAGATAGAAC+AGG + chr1_3:58165622-58165641 None:intergenic 35.0%
TCCACAATGACAAAGGAAAA+AGG + chr1_3:58165810-58165829 None:intergenic 35.0%
TGAAATGTCCACAATGACAA+AGG + chr1_3:58165817-58165836 None:intergenic 35.0%
AAGTAAGCGTAACAGAGATA+AGG - chr1_3:58165968-58165987 Msa0128440:intron 35.0%
AGTAAGCGTAACAGAGATAA+GGG - chr1_3:58165969-58165988 Msa0128440:intron 35.0%
AGCACCTATAGTGTAAAAGA+TGG - chr1_3:58166070-58166089 Msa0128440:intron 35.0%
TGGTAAAAAATAGGCTTAGG+TGG - chr1_3:58166090-58166109 Msa0128440:intron 35.0%
GGTAAAAAATAGGCTTAGGT+GGG - chr1_3:58166091-58166110 Msa0128440:intron 35.0%
CTTCGTACTACAACATCTAT+CGG + chr1_3:58166137-58166156 None:intergenic 35.0%
GATCCAAATATGGTTTCAGA+TGG - chr1_3:58166257-58166276 Msa0128440:intron 35.0%
! GTTCCATCTGAAACCATATT+TGG + chr1_3:58166263-58166282 None:intergenic 35.0%
TGGTTTCAGATGGAACATTA+TGG - chr1_3:58166267-58166286 Msa0128440:intron 35.0%
CATACCTTGAATCATATCTC+TGG - chr1_3:58166590-58166609 Msa0128440:CDS 35.0%
! GATGAAACAAAGGCATAAAG+TGG - chr1_3:58166713-58166732 Msa0128440:CDS 35.0%
! AATAGTATTGGAGAGGAAGT+TGG - chr1_3:58166744-58166763 Msa0128440:intron 35.0%
! ATAGTATTGGAGAGGAAGTT+GGG - chr1_3:58166745-58166764 Msa0128440:intron 35.0%
AAATGACATGATGTTGGCAA+GGG + chr1_3:58166856-58166875 None:intergenic 35.0%
GAGATACTTGTATGTGCAAT+TGG - chr1_3:58166953-58166972 Msa0128440:CDS 35.0%
! TGTACTATCAAAGCATCACA+CGG - chr1_3:58166981-58167000 Msa0128440:CDS 35.0%
! TTTGACTACAGAAAGGTACA+TGG + chr1_3:58167089-58167108 None:intergenic 35.0%
ATGTACCTTTCTGTAGTCAA+AGG - chr1_3:58167088-58167107 Msa0128440:five_prime_UTR 35.0%
GTATACCTTTGACTACAGAA+AGG + chr1_3:58167096-58167115 None:intergenic 35.0%
TTAGCATGTGGGGAATAAAA+CGG - chr1_3:58167210-58167229 Msa0128440:five_prime_UTR 35.0%
!! GTTCTGAGAAAGGAGTAATG+AGG + chr1_3:58164031-58164050 None:intergenic 40.0%
TGGTGAGAAAATTACTCCGA+TGG + chr1_3:58164122-58164141 None:intergenic 40.0%
! TTTGAGGTAAGTGGAAAGAG+TGG + chr1_3:58164167-58164186 None:intergenic 40.0%
! CTAGTGGAAGAAGTTTTCCA+CGG + chr1_3:58164483-58164502 None:intergenic 40.0%
CAGAGATCAAGAAACCAAGT+TGG + chr1_3:58164515-58164534 None:intergenic 40.0%
ACGGGTATAACCTCTCTATA+AGG - chr1_3:58164735-58164754 Msa0128440:intron 40.0%
GAAACTACTTAGGCCATGTT+TGG - chr1_3:58164792-58164811 Msa0128440:intron 40.0%
AGACGTGTCATAAGATATCC+TGG - chr1_3:58164903-58164922 Msa0128440:intron 40.0%
CAATTGAATCAACTAAGGCC+TGG - chr1_3:58165215-58165234 Msa0128440:intron 40.0%
! AGGAATCAACATTTAAGGCC+AGG + chr1_3:58165236-58165255 None:intergenic 40.0%
CCATCCCTCTAATTACTAAC+AGG + chr1_3:58165256-58165275 None:intergenic 40.0%
CCTGTTAGTAATTAGAGGGA+TGG - chr1_3:58165253-58165272 Msa0128440:intron 40.0%
AGCAAGCATCTTCGTTATTC+TGG + chr1_3:58165483-58165502 None:intergenic 40.0%
! GCCTTTTTCCTTTGTCATTG+TGG - chr1_3:58165806-58165825 Msa0128440:intron 40.0%
CTTCATACCATACAACATCG+TGG + chr1_3:58165917-58165936 None:intergenic 40.0%
TGGTAACTGGTAAGACTAGA+CGG - chr1_3:58166008-58166027 Msa0128440:intron 40.0%
ATGACACCATTAGAGAGAGT+TGG - chr1_3:58166044-58166063 Msa0128440:intron 40.0%
TGACACCATTAGAGAGAGTT+GGG - chr1_3:58166045-58166064 Msa0128440:intron 40.0%
AAAAATAGGCTTAGGTGGGT+TGG - chr1_3:58166095-58166114 Msa0128440:intron 40.0%
! AAAATAGGCTTAGGTGGGTT+GGG - chr1_3:58166096-58166115 Msa0128440:intron 40.0%
AGTACGAAGAGTAGATCAGA+TGG - chr1_3:58166148-58166167 Msa0128440:intron 40.0%
TCGGCTACATGGATCAAATT+AGG + chr1_3:58166293-58166312 None:intergenic 40.0%
TAGAAGTGAGCAGCAACTAT+TGG + chr1_3:58166650-58166669 None:intergenic 40.0%
GTGGAGAGAGAGAATAGTAT+TGG - chr1_3:58166732-58166751 Msa0128440:intron 40.0%
! GAGAGAGAATAGTATTGGAG+AGG - chr1_3:58166737-58166756 Msa0128440:intron 40.0%
CAAATGACATGATGTTGGCA+AGG + chr1_3:58166857-58166876 None:intergenic 40.0%
CTTGCCAACATCATGTCATT+TGG - chr1_3:58166855-58166874 Msa0128440:CDS 40.0%
GCTTCCAAATGACATGATGT+TGG + chr1_3:58166862-58166881 None:intergenic 40.0%
! AGTACCAAGAGTGTATTGCA+AGG + chr1_3:58166884-58166903 None:intergenic 40.0%
TGCAATACACTCTTGGTACT+TGG - chr1_3:58166884-58166903 Msa0128440:CDS 40.0%
AGTATCTCTACTAGAATGGG+AGG + chr1_3:58166941-58166960 None:intergenic 40.0%
! TTTCTCAGTGTGCTATTCTC+TGG - chr1_3:58167156-58167175 Msa0128440:five_prime_UTR 40.0%
!! TGGTGTTCTGGTTCTGAGAA+AGG + chr1_3:58164041-58164060 None:intergenic 45.0%
! AACCGCGGTTGTTATTAGAG+CGG + chr1_3:58164089-58164108 None:intergenic 45.0%
GATGGCTAAGAAAACAACCG+CGG + chr1_3:58164104-58164123 None:intergenic 45.0%
!! GCGGTTGTTTTCTTAGCCAT+CGG - chr1_3:58164103-58164122 Msa0128440:three_prime_UTR 45.0%
! ATGAGCGAGTTTGAGGTAAG+TGG + chr1_3:58164176-58164195 None:intergenic 45.0%
TGTTTGCATTGCTGTCACTG+TGG - chr1_3:58164437-58164456 Msa0128440:intron 45.0%
CCACGGATGAAGATAACCAA+AGG + chr1_3:58164466-58164485 None:intergenic 45.0%
CCTTTGGTTATCTTCATCCG+TGG - chr1_3:58164463-58164482 Msa0128440:intron 45.0%
CTGCGAAGCTTACTTTAAGC+TGG - chr1_3:58164770-58164789 Msa0128440:intron 45.0%
AGACGGTTTGAGAGAGCTTA+CGG + chr1_3:58164983-58165002 None:intergenic 45.0%
CGGTATAAGTGCTCATGAGA+CGG + chr1_3:58165000-58165019 None:intergenic 45.0%
CGTCTCATGAGCACTTATAC+CGG - chr1_3:58164998-58165017 Msa0128440:intron 45.0%
TCATGAGCACTTATACCGGT+CGG - chr1_3:58165002-58165021 Msa0128440:intron 45.0%
ACAGGAAATGAGGACAGAAG+AGG + chr1_3:58165604-58165623 None:intergenic 45.0%
TGTTGCATCAGTGCAAGTTC+GGG + chr1_3:58165649-58165668 None:intergenic 45.0%
CTGTTGCATCAGTGCAAGTT+CGG + chr1_3:58165650-58165669 None:intergenic 45.0%
AGTCAGACCACGATGTTGTA+TGG - chr1_3:58165907-58165926 Msa0128440:intron 45.0%
CGCTTACTTGATTCTCGTGT+TGG + chr1_3:58165957-58165976 None:intergenic 45.0%
GGTAACTGGTAAGACTAGAC+GGG - chr1_3:58166009-58166028 Msa0128440:intron 45.0%
AGACTAGACGGGATAGGATT+AGG - chr1_3:58166020-58166039 Msa0128440:intron 45.0%
GACACCATTAGAGAGAGTTG+GGG - chr1_3:58166046-58166065 Msa0128440:intron 45.0%
AAGCATCATCCCACTAAGTG+GGG + chr1_3:58166316-58166335 None:intergenic 45.0%
CAAGCATCATCCCACTAAGT+GGG + chr1_3:58166317-58166336 None:intergenic 45.0%
GAAGCCTTGCAATACACTCT+TGG - chr1_3:58166877-58166896 Msa0128440:CDS 45.0%
ATACACTCTTGGTACTTGGC+AGG - chr1_3:58166888-58166907 Msa0128440:CDS 45.0%
ATGTTTGCACAGCAAGACAC+CGG + chr1_3:58167027-58167046 None:intergenic 45.0%
AGCCGCTCTAATAACAACCG+CGG - chr1_3:58164084-58164103 Msa0128440:three_prime_UTR 50.0%
GAGGTAAGTGGAAAGAGTGG+TGG + chr1_3:58164164-58164183 None:intergenic 50.0%
CTCAAACTCGCTCATCTCCT+CGG - chr1_3:58164181-58164200 Msa0128440:exon 50.0%
GATGAAGGTGACCCATAGAG+CGG + chr1_3:58164227-58164246 None:intergenic 50.0%
GCTCTATGGGTCACCTTCAT+CGG - chr1_3:58164226-58164245 Msa0128440:CDS 50.0%
CATGATGATACCGCCGATGA+AGG + chr1_3:58164242-58164261 None:intergenic 50.0%
! GGCTTTTTGAACTTGCCGAC+CGG + chr1_3:58165020-58165039 None:intergenic 50.0%
TTGCATCAGTGCAAGTTCGG+GGG + chr1_3:58165647-58165666 None:intergenic 50.0%
GTTGCATCAGTGCAAGTTCG+GGG + chr1_3:58165648-58165667 None:intergenic 50.0%
!! CAGAGATAAGGGTGTTGCGT+TGG - chr1_3:58165980-58165999 Msa0128440:intron 50.0%
TGCGTTGGATGAGTGGTAAC+TGG - chr1_3:58165995-58166014 Msa0128440:intron 50.0%
CTGGTAAGACTAGACGGGAT+AGG - chr1_3:58166014-58166033 Msa0128440:intron 50.0%
GCTACCCCAACTCTCTCTAA+TGG + chr1_3:58166053-58166072 None:intergenic 50.0%
ATCATCCCACTAAGTGGGGT+CGG + chr1_3:58166312-58166331 None:intergenic 50.0%
CCAAGCATCATCCCACTAAG+TGG + chr1_3:58166318-58166337 None:intergenic 50.0%
CCACTTAGTGGGATGATGCT+TGG - chr1_3:58166315-58166334 Msa0128440:intron 50.0%
GTATTGGAGAGGAAGTTGGG+TGG - chr1_3:58166748-58166767 Msa0128440:intron 50.0%
AGCAAGACACCGGATACTCT+TGG + chr1_3:58167017-58167036 None:intergenic 50.0%
AACCAGATTCCAGCGCTCGT+GGG + chr1_3:58163931-58163950 None:intergenic 55.0%
AAACCAGATTCCAGCGCTCG+TGG + chr1_3:58163932-58163951 None:intergenic 55.0%
!! ACGAGCGCTGGAATCTGGTT+TGG - chr1_3:58163931-58163950 Msa0128440:three_prime_UTR 55.0%
ACCACCACCACCGTCATCAA+TGG - chr1_3:58164057-58164076 Msa0128440:three_prime_UTR 55.0%
CCACCACCACCGTCATCAAT+GGG - chr1_3:58164058-58164077 Msa0128440:three_prime_UTR 55.0%
!! CTACCCATTGATGACGGTGG+TGG + chr1_3:58164064-58164083 None:intergenic 55.0%
!! GCTCTACCCATTGATGACGG+TGG + chr1_3:58164067-58164086 None:intergenic 55.0%
GCGGCTCTACCCATTGATGA+CGG + chr1_3:58164070-58164089 None:intergenic 55.0%
CGAGGAGATGAGCGAGTTTG+AGG + chr1_3:58164183-58164202 None:intergenic 55.0%
CTCGGCTTCTCCATCGCTTT+CGG - chr1_3:58164199-58164218 Msa0128440:CDS 55.0%
CTATGGGTCACCTTCATCGG+CGG - chr1_3:58164229-58164248 Msa0128440:CDS 55.0%
GCTGTCACTGTGGCTTCCTT+TGG - chr1_3:58164447-58164466 Msa0128440:intron 55.0%
!! AGGGTGTTGCGTTGGATGAG+TGG - chr1_3:58165988-58166007 Msa0128440:intron 55.0%
ACTAAGTGGGGTCGGCTACA+TGG + chr1_3:58166304-58166323 None:intergenic 55.0%
ATGTAGCCGACCCCACTTAG+TGG - chr1_3:58166303-58166322 Msa0128440:intron 55.0%
TGTAGCCGACCCCACTTAGT+GGG - chr1_3:58166304-58166323 Msa0128440:intron 55.0%
CAATCCAAACCCACGAGCGC+TGG - chr1_3:58163919-58163938 Msa0128440:three_prime_UTR 60.0%
GATTCCAGCGCTCGTGGGTT+TGG + chr1_3:58163926-58163945 None:intergenic 60.0%
!! AACCCACGAGCGCTGGAATC+TGG - chr1_3:58163926-58163945 Msa0128440:three_prime_UTR 60.0%
!! TGACGGTGGTGGTGGTGTTC+TGG + chr1_3:58164053-58164072 None:intergenic 60.0%
!! CCCATTGATGACGGTGGTGG+TGG + chr1_3:58164061-58164080 None:intergenic 60.0%
CGAAAGCGATGGAGAAGCCG+AGG + chr1_3:58164201-58164220 None:intergenic 60.0%
! TAGAGCGGCGCCGAAAGCGA+TGG + chr1_3:58164212-58164231 None:intergenic 65.0%
TCGCTTTCGGCGCCGCTCTA+TGG - chr1_3:58164212-58164231 Msa0128440:CDS 65.0%
CGCTTTCGGCGCCGCTCTAT+GGG - chr1_3:58164213-58164232 Msa0128440:CDS 65.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 58163861 58167255 58163861 ID=Msa0128440;Name=Msa0128440
chr1_3 mRNA 58163861 58167255 58163861 ID=Msa0128440-mRNA-1;Parent=Msa0128440;Name=Msa0128440-mRNA-1;_AED=0.06;_eAED=0.06;_QI=215|1|1|1|1|1|3|329|208
chr1_3 exon 58163861 58164405 58163861 ID=Msa0128440-mRNA-1:exon:18650;Parent=Msa0128440-mRNA-1
chr1_3 exon 58166529 58166748 58166529 ID=Msa0128440-mRNA-1:exon:18649;Parent=Msa0128440-mRNA-1
chr1_3 exon 58166850 58167255 58166850 ID=Msa0128440-mRNA-1:exon:18648;Parent=Msa0128440-mRNA-1
chr1_3 five_prime_UTR 58167041 58167255 58167041 ID=Msa0128440-mRNA-1:five_prime_utr;Parent=Msa0128440-mRNA-1
chr1_3 CDS 58166850 58167040 58166850 ID=Msa0128440-mRNA-1:cds;Parent=Msa0128440-mRNA-1
chr1_3 CDS 58166529 58166748 58166529 ID=Msa0128440-mRNA-1:cds;Parent=Msa0128440-mRNA-1
chr1_3 CDS 58164190 58164405 58164190 ID=Msa0128440-mRNA-1:cds;Parent=Msa0128440-mRNA-1
chr1_3 three_prime_UTR 58163861 58164189 58163861 ID=Msa0128440-mRNA-1:three_prime_utr;Parent=Msa0128440-mRNA-1
Gene Sequence

>Msa0128440

ATGGGTAGAGCCGCTCTAATAACAACCGCGGTTGTTTTCTTAGCCATCGGAGTAATTTTCTCACCAGAAACTTTCGGATCAAAATCCACCACTCTTTCCACTTACCTCAAACTCGCTCATCTCCTCGGCTTCTCCATCGCTTTCGGCGCCGCTCTATGGGTCACCTTCATCGGCGGTATCATCATGTTCAAGAATTTGCCTAGGCATCAATTTGGTAATCTTCAAAGCAAAATGTTTCCAGCTTATTTTTCAATGGTTGGTGTTTGCATTGCTGTCACTGTGGCTTCCTTTGGTTATCTTCATCCGTGGAAAACTTCTTCCACTAGTGAAAGATACCAACTTGGTTTCTTGATCTCTGCTCTTGCTTTCAATCTTACCAATTTGTTCGTCTTTACACCAATGACAATTGAGATGATGAAACAAAGGCATAAAGTGGAGAGAGAGAATAGTATTGGAGAGGAAGTTGGGTGGTCAAAAAATGTCGAAGTTGCAAAGAAAAATCCAAAGCTTGCAAAGATGAATAAGAAGTTTGGTATGATTCATGGGTTATCTTCCCTTGCCAACATCATGTCATTTGGAAGCCTTGCAATACACTCTTGGTACTTGGCAGGTAAACTTGATCTCTGA
Protein sequence

>Msa0128440

MGRAALITTAVVFLAIGVIFSPETFGSKSTTLSTYLKLAHLLGFSIAFGAALWVTFIGGIIMFKNLPRHQFGNLQSKMFPAYFSMVGVCIAVTVASFGYLHPWKTSSTSERYQLGFLISALAFNLTNLFVFTPMTIEMMKQRHKVERENSIGEEVGWSKNVEVAKKNPKLAKMNKKFGMIHGLSSLANIMSFGSLAIHSWYLAGKLDL*