AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0128790

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0128790 MtrunA17_Chr1g0195681 98.824 170 2 0 1 170 1 170 2.34e-122 342
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0128790 AT1G02870.1 55.959 193 61 4 1 169 1 193 1.80e-67 204

Find 60 sgRNAs with CRISPR-Local

Find 227 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATAGCTTTCTAAAGTTAAT+TGG 0.136445 1_3:+58637535 Msa0128790:three_prime_UTR
CGAGTATGAGATTAGGGTTT+AGG 0.234991 1_3:-58636007 None:intergenic
AAAGCGGGTTTCAGAACTTT+AGG 0.241847 1_3:-58636146 None:intergenic
CCCTTGCAGAAGATCCTAAA+TGG 0.241943 1_3:+58636195 Msa0128790:CDS
TTAATAGATGAAAGTAGTTT+TGG 0.244162 1_3:+58637729 Msa0128790:three_prime_UTR
GAGCCTTTATCGTCCCATTT+AGG 0.265744 1_3:-58636209 None:intergenic
TGAAATCGGCGTTGGGTAAA+AGG 0.283106 1_3:+58636461 Msa0128790:CDS
GCAAGGGATTGTAACAGTTT+TGG 0.284108 1_3:-58636179 None:intergenic
CGCCCAGATTGTAAATTTGA+TGG 0.304006 1_3:-58637482 None:intergenic
CGCCGAAAGAGGAATAGTTT+TGG 0.317209 1_3:-58636238 None:intergenic
AGAACTTTAGGGTTCTTCTT+TGG 0.324436 1_3:-58636134 None:intergenic
TTGGGAAAGATGTAGTATTC+TGG 0.325763 1_3:+58637677 Msa0128790:three_prime_UTR
TGGAATGTGATAAGTTGTTT+TGG 0.328418 1_3:+58637753 Msa0128790:three_prime_UTR
GAGTATGAGATTAGGGTTTA+GGG 0.339426 1_3:-58636006 None:intergenic
ACCCAAAACTATTCCTCTTT+CGG 0.353542 1_3:+58636236 Msa0128790:CDS
GGAAGACCAACACGTACCTT+TGG 0.363935 1_3:-58636113 None:intergenic
ATGCCATCAAATTTACAATC+TGG 0.368534 1_3:+58637479 Msa0128790:three_prime_UTR
AGAGAATCAGTAAGGGAGTT+AGG 0.373552 1_3:-58636272 None:intergenic
TGCAACTGCGTAAGCAAGAT+AGG 0.378065 1_3:-58636036 None:intergenic
ATAGCTTTCTAAAGTTAATT+GGG 0.414342 1_3:+58637536 Msa0128790:three_prime_UTR
AAGCGGGTTTCAGAACTTTA+GGG 0.416206 1_3:-58636145 None:intergenic
GAAGACCAACACGTACCTTT+GGG 0.427653 1_3:-58636112 None:intergenic
GATCCTAAATGGGACGATAA+AGG 0.428418 1_3:+58636206 Msa0128790:CDS
GACAAGAATCCGCTCATTGT+TGG 0.441943 1_3:+58637424 Msa0128790:CDS
TTGTGGTGTAGATTTGAAAT+CGG 0.469021 1_3:+58636447 Msa0128790:intron
GAATTTGAAGAGAATCAGTA+AGG 0.477015 1_3:-58636280 None:intergenic
TGCCATCAAATTTACAATCT+GGG 0.479882 1_3:+58637480 Msa0128790:three_prime_UTR
GTCTTCTTCAAGATCACTGC+CGG 0.487579 1_3:-58636327 None:intergenic
AGATACAGAGAGAAGAAGAA+TGG 0.487957 1_3:+58636058 Msa0128790:exon
GATACAGAGAGAAGAAGAAT+GGG 0.488189 1_3:+58636059 Msa0128790:exon
GTAGATTTGAAATCGGCGTT+GGG 0.505626 1_3:+58636454 Msa0128790:intron
TCCATGCAAAATTCAAAAGC+AGG 0.510757 1_3:-58637648 None:intergenic
TCAAATTTACAATCTGGGCG+TGG 0.510966 1_3:+58637485 Msa0128790:three_prime_UTR
ATGGCATTGCACTTGTCCAT+AGG 0.520593 1_3:-58637463 None:intergenic
TGAATTGAAGTCAAAGGTTG+AGG 0.526816 1_3:-58636508 None:intergenic
TGTAGATTTGAAATCGGCGT+TGG 0.527237 1_3:+58636453 Msa0128790:intron
ATCAGAAACAACGCCGAAAG+AGG 0.531608 1_3:-58636249 None:intergenic
GCGTGGGTTGTGAGCTGCAA+CGG 0.531681 1_3:+58637502 Msa0128790:three_prime_UTR
GTTGCAACTGAATGCTGCAT+AGG 0.533068 1_3:-58637364 None:intergenic
TTGCAACTGAATGCTGCATA+GGG 0.539277 1_3:-58637363 None:intergenic
CCTTGCAGAAGATCCTAAAT+GGG 0.546570 1_3:+58636196 Msa0128790:CDS
CCATTTAGGATCTTCTGCAA+GGG 0.547386 1_3:-58636195 None:intergenic
TAACTTTAGAAAGCTATCCA+TGG 0.552988 1_3:-58637530 None:intergenic
CCCATTTAGGATCTTCTGCA+AGG 0.554925 1_3:-58636196 None:intergenic
GTTGCCGATGATAACGATCC+CGG 0.559862 1_3:+58636308 Msa0128790:CDS
CAAATTTACAATCTGGGCGT+GGG 0.560830 1_3:+58637486 Msa0128790:three_prime_UTR
TTTGGAGGAATAGTGAAAGC+GGG 0.577554 1_3:-58636161 None:intergenic
GCAACTGCGTAAGCAAGATA+GGG 0.596037 1_3:-58636035 None:intergenic
TCTTCTTCAAGATCACTGCC+GGG 0.597126 1_3:-58636326 None:intergenic
TCGTTATCATCGGCAACAGA+AGG 0.598477 1_3:-58636302 None:intergenic
AGACGCTGAATTGAAGTCAA+AGG 0.608242 1_3:-58636514 None:intergenic
GAGCTGCAACGGTTGATCCA+TGG 0.610724 1_3:+58637513 Msa0128790:three_prime_UTR
AATTTGAAGAGAATCAGTAA+GGG 0.613175 1_3:-58636279 None:intergenic
TTGGGTAAAAGGAGAAGAGA+TGG 0.616129 1_3:+58636472 Msa0128790:CDS
TTTCATTTACCAACAATGAG+CGG 0.625317 1_3:-58637433 None:intergenic
GGCAGTGATCTTGAAGAAGA+CGG 0.629513 1_3:+58636329 Msa0128790:CDS
TCTCGCCCAAAGGTACGTGT+TGG 0.632503 1_3:+58636107 Msa0128790:CDS
GTATAAGCAATCTCGCCCAA+AGG 0.632811 1_3:+58636097 Msa0128790:CDS
ACTGCCGGGATCGTTATCAT+CGG 0.664050 1_3:-58636312 None:intergenic
AGCAAAATGTTGTTACAATG+AGG 0.667627 1_3:+58637566 Msa0128790:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTGTTTTGTTTTTATTT+TGG + chr1_3:58637311-58637330 Msa0128790:intron 10.0%
!!! TTATTGTTTTGTTTTTATTT+TGG + chr1_3:58637311-58637330 Msa0128790:intron 10.0%
!! AACTAATTTATTTGCATAAA+CGG - chr1_3:58636902-58636921 None:intergenic 15.0%
!! AGTAAAATGTTTATGATTAA+GGG - chr1_3:58637091-58637110 None:intergenic 15.0%
!!! CTTTCATCTATTAATTTTTT+TGG - chr1_3:58637723-58637742 None:intergenic 15.0%
!! AACTAATTTATTTGCATAAA+CGG - chr1_3:58636902-58636921 None:intergenic 15.0%
!! AGTAAAATGTTTATGATTAA+GGG - chr1_3:58637091-58637110 None:intergenic 15.0%
!!! CTTTCATCTATTAATTTTTT+TGG - chr1_3:58637723-58637742 None:intergenic 15.0%
!! GTTGAAATTAGTATTAATCT+TGG + chr1_3:58636388-58636407 Msa0128790:intron 20.0%
!! GAATGTAAATAATTTGAGTA+TGG + chr1_3:58636422-58636441 Msa0128790:intron 20.0%
!!! TTTATCTTTTTCTATAGAAG+TGG - chr1_3:58636718-58636737 None:intergenic 20.0%
!!! TAAATTTTGTGAGATTGTTT+GGG - chr1_3:58636832-58636851 None:intergenic 20.0%
!!! ATAAATTTTGTGAGATTGTT+TGG - chr1_3:58636833-58636852 None:intergenic 20.0%
!!! TGTTTTTTCATAAATAACCT+TGG - chr1_3:58636949-58636968 None:intergenic 20.0%
!! GAGTAAAATGTTTATGATTA+AGG - chr1_3:58637092-58637111 None:intergenic 20.0%
!!! CATTTTACTCATATACTATA+TGG + chr1_3:58637102-58637121 Msa0128790:intron 20.0%
!! TATCATAATCTTGAAAACAT+CGG + chr1_3:58637159-58637178 Msa0128790:intron 20.0%
!!! ATAGCTTTCTAAAGTTAATT+GGG + chr1_3:58637536-58637555 Msa0128790:three_prime_UTR 20.0%
!!! TTAATAGATGAAAGTAGTTT+TGG + chr1_3:58637729-58637748 Msa0128790:three_prime_UTR 20.0%
!! GTTGAAATTAGTATTAATCT+TGG + chr1_3:58636388-58636407 Msa0128790:intron 20.0%
!! GAATGTAAATAATTTGAGTA+TGG + chr1_3:58636422-58636441 Msa0128790:intron 20.0%
!!! TTTATCTTTTTCTATAGAAG+TGG - chr1_3:58636718-58636737 None:intergenic 20.0%
!!! TAAATTTTGTGAGATTGTTT+GGG - chr1_3:58636832-58636851 None:intergenic 20.0%
!!! ATAAATTTTGTGAGATTGTT+TGG - chr1_3:58636833-58636852 None:intergenic 20.0%
!!! TGTTTTTTCATAAATAACCT+TGG - chr1_3:58636949-58636968 None:intergenic 20.0%
!! GAGTAAAATGTTTATGATTA+AGG - chr1_3:58637092-58637111 None:intergenic 20.0%
!!! CATTTTACTCATATACTATA+TGG + chr1_3:58637102-58637121 Msa0128790:intron 20.0%
!! TATCATAATCTTGAAAACAT+CGG + chr1_3:58637159-58637178 Msa0128790:intron 20.0%
!!! ATAGCTTTCTAAAGTTAATT+GGG + chr1_3:58637536-58637555 Msa0128790:three_prime_UTR 20.0%
!!! TTAATAGATGAAAGTAGTTT+TGG + chr1_3:58637729-58637748 Msa0128790:three_prime_UTR 20.0%
! AATTTGAAGAGAATCAGTAA+GGG - chr1_3:58636282-58636301 None:intergenic 25.0%
! TAACAATTTACGATGAATTG+AGG - chr1_3:58636361-58636380 None:intergenic 25.0%
!! ATTAATCTTGGTAATTCTGA+AGG + chr1_3:58636400-58636419 Msa0128790:intron 25.0%
!!! ATAATTTGAGTATGGTTTTG+TGG + chr1_3:58636430-58636449 Msa0128790:intron 25.0%
! AGTCGTTTAGTTGAAAAATA+TGG + chr1_3:58636544-58636563 Msa0128790:CDS 25.0%
!! AAAATATGGAGATGATTTTC+AGG + chr1_3:58636558-58636577 Msa0128790:CDS 25.0%
!!! GATAATTTGTTTATCCAAAC+AGG - chr1_3:58636657-58636676 None:intergenic 25.0%
! TATGAAAAAACAGCTTATGA+AGG + chr1_3:58636957-58636976 Msa0128790:intron 25.0%
! TACTCATATACTATATGGTT+AGG + chr1_3:58637107-58637126 Msa0128790:intron 25.0%
!! TAAATGAAATGTTTTGCCTA+TGG + chr1_3:58637447-58637466 Msa0128790:exon 25.0%
!!! GATAGCTTTCTAAAGTTAAT+TGG + chr1_3:58637535-58637554 Msa0128790:three_prime_UTR 25.0%
!! AATTTTGCATGGATTTTTGT+TGG + chr1_3:58637658-58637677 Msa0128790:three_prime_UTR 25.0%
!! ATTTTGCATGGATTTTTGTT+GGG + chr1_3:58637659-58637678 Msa0128790:three_prime_UTR 25.0%
!! TTTTGGCAACAACAATTTTT+GGG - chr1_3:58637706-58637725 None:intergenic 25.0%
!!! TTTTTGGCAACAACAATTTT+TGG - chr1_3:58637707-58637726 None:intergenic 25.0%
! AATTTGAAGAGAATCAGTAA+GGG - chr1_3:58636282-58636301 None:intergenic 25.0%
! TAACAATTTACGATGAATTG+AGG - chr1_3:58636361-58636380 None:intergenic 25.0%
!! ATTAATCTTGGTAATTCTGA+AGG + chr1_3:58636400-58636419 Msa0128790:intron 25.0%
!!! ATAATTTGAGTATGGTTTTG+TGG + chr1_3:58636430-58636449 Msa0128790:intron 25.0%
! AGTCGTTTAGTTGAAAAATA+TGG + chr1_3:58636544-58636563 Msa0128790:CDS 25.0%
!! AAAATATGGAGATGATTTTC+AGG + chr1_3:58636558-58636577 Msa0128790:CDS 25.0%
!!! GATAATTTGTTTATCCAAAC+AGG - chr1_3:58636657-58636676 None:intergenic 25.0%
! TATGAAAAAACAGCTTATGA+AGG + chr1_3:58636957-58636976 Msa0128790:intron 25.0%
! TACTCATATACTATATGGTT+AGG + chr1_3:58637107-58637126 Msa0128790:intron 25.0%
!! TAAATGAAATGTTTTGCCTA+TGG + chr1_3:58637447-58637466 Msa0128790:exon 25.0%
!!! GATAGCTTTCTAAAGTTAAT+TGG + chr1_3:58637535-58637554 Msa0128790:three_prime_UTR 25.0%
!! AATTTTGCATGGATTTTTGT+TGG + chr1_3:58637658-58637677 Msa0128790:three_prime_UTR 25.0%
!! ATTTTGCATGGATTTTTGTT+GGG + chr1_3:58637659-58637678 Msa0128790:three_prime_UTR 25.0%
!! TTTTGGCAACAACAATTTTT+GGG - chr1_3:58637706-58637725 None:intergenic 25.0%
!!! TTTTTGGCAACAACAATTTT+TGG - chr1_3:58637707-58637726 None:intergenic 25.0%
!!! GTTTTTCGAGTATGAGATTA+GGG - chr1_3:58636016-58636035 None:intergenic 30.0%
GAATTTGAAGAGAATCAGTA+AGG - chr1_3:58636283-58636302 None:intergenic 30.0%
! TTGTGGTGTAGATTTGAAAT+CGG + chr1_3:58636447-58636466 Msa0128790:intron 30.0%
TCTCACAAAATTTATGTCAG+TGG + chr1_3:58636838-58636857 Msa0128790:intron 30.0%
TATTATTCATAAGCTTGCCA+AGG + chr1_3:58636929-58636948 Msa0128790:intron 30.0%
! TCTAAAGTAACACAAATGAG+TGG + chr1_3:58637198-58637217 Msa0128790:intron 30.0%
! CAAATGTTACTCTGTTTTCT+TGG + chr1_3:58637262-58637281 Msa0128790:intron 30.0%
!! TACTCTGTTTTCTTGGTTTT+AGG + chr1_3:58637269-58637288 Msa0128790:intron 30.0%
!!! GTTTTAGGACTTCATTTATG+TGG + chr1_3:58637284-58637303 Msa0128790:intron 30.0%
!! ATTTTGGCAGAAAATGATGA+TGG + chr1_3:58637327-58637346 Msa0128790:intron 30.0%
TTTCATTTACCAACAATGAG+CGG - chr1_3:58637436-58637455 None:intergenic 30.0%
ATGCCATCAAATTTACAATC+TGG + chr1_3:58637479-58637498 Msa0128790:three_prime_UTR 30.0%
TGCCATCAAATTTACAATCT+GGG + chr1_3:58637480-58637499 Msa0128790:three_prime_UTR 30.0%
! TAACTTTAGAAAGCTATCCA+TGG - chr1_3:58637533-58637552 None:intergenic 30.0%
AGCAAAATGTTGTTACAATG+AGG + chr1_3:58637566-58637585 Msa0128790:three_prime_UTR 30.0%
!!! TAGATGAAAGTAGTTTTGGA+TGG + chr1_3:58637733-58637752 Msa0128790:three_prime_UTR 30.0%
! TGGAATGTGATAAGTTGTTT+TGG + chr1_3:58637753-58637772 Msa0128790:three_prime_UTR 30.0%
!! GGAATGTGATAAGTTGTTTT+GGG + chr1_3:58637754-58637773 Msa0128790:three_prime_UTR 30.0%
!!! GTTTTTCGAGTATGAGATTA+GGG - chr1_3:58636016-58636035 None:intergenic 30.0%
GAATTTGAAGAGAATCAGTA+AGG - chr1_3:58636283-58636302 None:intergenic 30.0%
! TTGTGGTGTAGATTTGAAAT+CGG + chr1_3:58636447-58636466 Msa0128790:intron 30.0%
TCTCACAAAATTTATGTCAG+TGG + chr1_3:58636838-58636857 Msa0128790:intron 30.0%
TATTATTCATAAGCTTGCCA+AGG + chr1_3:58636929-58636948 Msa0128790:intron 30.0%
! TCTAAAGTAACACAAATGAG+TGG + chr1_3:58637198-58637217 Msa0128790:intron 30.0%
! CAAATGTTACTCTGTTTTCT+TGG + chr1_3:58637262-58637281 Msa0128790:intron 30.0%
!! TACTCTGTTTTCTTGGTTTT+AGG + chr1_3:58637269-58637288 Msa0128790:intron 30.0%
!!! GTTTTAGGACTTCATTTATG+TGG + chr1_3:58637284-58637303 Msa0128790:intron 30.0%
!! ATTTTGGCAGAAAATGATGA+TGG + chr1_3:58637327-58637346 Msa0128790:intron 30.0%
TTTCATTTACCAACAATGAG+CGG - chr1_3:58637436-58637455 None:intergenic 30.0%
ATGCCATCAAATTTACAATC+TGG + chr1_3:58637479-58637498 Msa0128790:three_prime_UTR 30.0%
TGCCATCAAATTTACAATCT+GGG + chr1_3:58637480-58637499 Msa0128790:three_prime_UTR 30.0%
! TAACTTTAGAAAGCTATCCA+TGG - chr1_3:58637533-58637552 None:intergenic 30.0%
AGCAAAATGTTGTTACAATG+AGG + chr1_3:58637566-58637585 Msa0128790:three_prime_UTR 30.0%
!!! TAGATGAAAGTAGTTTTGGA+TGG + chr1_3:58637733-58637752 Msa0128790:three_prime_UTR 30.0%
! TGGAATGTGATAAGTTGTTT+TGG + chr1_3:58637753-58637772 Msa0128790:three_prime_UTR 30.0%
GAGTATGAGATTAGGGTTTA+GGG - chr1_3:58636009-58636028 None:intergenic 35.0%
!!! GGTTTTTCGAGTATGAGATT+AGG - chr1_3:58636017-58636036 None:intergenic 35.0%
AGATACAGAGAGAAGAAGAA+TGG + chr1_3:58636058-58636077 Msa0128790:exon 35.0%
GATACAGAGAGAAGAAGAAT+GGG + chr1_3:58636059-58636078 Msa0128790:exon 35.0%
!! AGAACTTTAGGGTTCTTCTT+TGG - chr1_3:58636137-58636156 None:intergenic 35.0%
!! TTTTGGAGGAATAGTGAAAG+CGG - chr1_3:58636165-58636184 None:intergenic 35.0%
ACCCAAAACTATTCCTCTTT+CGG + chr1_3:58636236-58636255 Msa0128790:CDS 35.0%
TGAATTGAAGTCAAAGGTTG+AGG - chr1_3:58636511-58636530 None:intergenic 35.0%
!!! CGTTTTTTTCACTGTTGCTT+TGG + chr1_3:58636583-58636602 Msa0128790:intron 35.0%
! AGCTTTGATAGTTCTCTTCT+AGG + chr1_3:58636630-58636649 Msa0128790:intron 35.0%
ACTGTCCCAATAAACCTTTA+GGG + chr1_3:58636876-58636895 Msa0128790:intron 35.0%
!! TGAAGGTGTACAATTTTCGT+CGG + chr1_3:58636974-58636993 Msa0128790:intron 35.0%
! GATTAAGGGCTCTTTTTGAT+TGG - chr1_3:58637077-58637096 None:intergenic 35.0%
!! TCCTGCTTTTGAATTTTGCA+TGG + chr1_3:58637647-58637666 Msa0128790:three_prime_UTR 35.0%
TCCATGCAAAATTCAAAAGC+AGG - chr1_3:58637651-58637670 None:intergenic 35.0%
TTGGGAAAGATGTAGTATTC+TGG + chr1_3:58637677-58637696 Msa0128790:three_prime_UTR 35.0%
GAGTATGAGATTAGGGTTTA+GGG - chr1_3:58636009-58636028 None:intergenic 35.0%
!!! GGTTTTTCGAGTATGAGATT+AGG - chr1_3:58636017-58636036 None:intergenic 35.0%
AGATACAGAGAGAAGAAGAA+TGG + chr1_3:58636058-58636077 Msa0128790:exon 35.0%
GATACAGAGAGAAGAAGAAT+GGG + chr1_3:58636059-58636078 Msa0128790:exon 35.0%
!! AGAACTTTAGGGTTCTTCTT+TGG - chr1_3:58636137-58636156 None:intergenic 35.0%
!! TTTTGGAGGAATAGTGAAAG+CGG - chr1_3:58636165-58636184 None:intergenic 35.0%
ACCCAAAACTATTCCTCTTT+CGG + chr1_3:58636236-58636255 Msa0128790:CDS 35.0%
TGAATTGAAGTCAAAGGTTG+AGG - chr1_3:58636511-58636530 None:intergenic 35.0%
!!! CGTTTTTTTCACTGTTGCTT+TGG + chr1_3:58636583-58636602 Msa0128790:intron 35.0%
! AGCTTTGATAGTTCTCTTCT+AGG + chr1_3:58636630-58636649 Msa0128790:intron 35.0%
ACTGTCCCAATAAACCTTTA+GGG + chr1_3:58636876-58636895 Msa0128790:intron 35.0%
!! TGAAGGTGTACAATTTTCGT+CGG + chr1_3:58636974-58636993 Msa0128790:intron 35.0%
! GATTAAGGGCTCTTTTTGAT+TGG - chr1_3:58637077-58637096 None:intergenic 35.0%
!! TCCTGCTTTTGAATTTTGCA+TGG + chr1_3:58637647-58637666 Msa0128790:three_prime_UTR 35.0%
TCCATGCAAAATTCAAAAGC+AGG - chr1_3:58637651-58637670 None:intergenic 35.0%
TTGGGAAAGATGTAGTATTC+TGG + chr1_3:58637677-58637696 Msa0128790:three_prime_UTR 35.0%
CGAGTATGAGATTAGGGTTT+AGG - chr1_3:58636010-58636029 None:intergenic 40.0%
! AAGCGGGTTTCAGAACTTTA+GGG - chr1_3:58636148-58636167 None:intergenic 40.0%
! AAAGCGGGTTTCAGAACTTT+AGG - chr1_3:58636149-58636168 None:intergenic 40.0%
TTTGGAGGAATAGTGAAAGC+GGG - chr1_3:58636164-58636183 None:intergenic 40.0%
!! AGGGATTGTAACAGTTTTGG+AGG - chr1_3:58636179-58636198 None:intergenic 40.0%
! GCAAGGGATTGTAACAGTTT+TGG - chr1_3:58636182-58636201 None:intergenic 40.0%
! CCATTTAGGATCTTCTGCAA+GGG - chr1_3:58636198-58636217 None:intergenic 40.0%
CCTTGCAGAAGATCCTAAAT+GGG + chr1_3:58636196-58636215 Msa0128790:CDS 40.0%
GATCCTAAATGGGACGATAA+AGG + chr1_3:58636206-58636225 Msa0128790:CDS 40.0%
! GCCGAAAGAGGAATAGTTTT+GGG - chr1_3:58636240-58636259 None:intergenic 40.0%
AGAGAATCAGTAAGGGAGTT+AGG - chr1_3:58636275-58636294 None:intergenic 40.0%
! TGTAGATTTGAAATCGGCGT+TGG + chr1_3:58636453-58636472 Msa0128790:intron 40.0%
! GTAGATTTGAAATCGGCGTT+GGG + chr1_3:58636454-58636473 Msa0128790:intron 40.0%
TTGGGTAAAAGGAGAAGAGA+TGG + chr1_3:58636472-58636491 Msa0128790:CDS 40.0%
AGACGCTGAATTGAAGTCAA+AGG - chr1_3:58636517-58636536 None:intergenic 40.0%
GACTGTCCCAATAAACCTTT+AGG + chr1_3:58636875-58636894 Msa0128790:intron 40.0%
AACGGCCCTAAAGGTTTATT+GGG - chr1_3:58636884-58636903 None:intergenic 40.0%
AAACGGCCCTAAAGGTTTAT+TGG - chr1_3:58636885-58636904 None:intergenic 40.0%
ATTTGCATAAACGGCCCTAA+AGG - chr1_3:58636893-58636912 None:intergenic 40.0%
!! GAAGGTGTACAATTTTCGTC+GGG + chr1_3:58636975-58636994 Msa0128790:intron 40.0%
! AAGGTGTACAATTTTCGTCG+GGG + chr1_3:58636976-58636995 Msa0128790:intron 40.0%
!! TGAGTGGTTGTGATTTGTTG+AGG + chr1_3:58637214-58637233 Msa0128790:intron 40.0%
TTGCAACTGAATGCTGCATA+GGG - chr1_3:58637366-58637385 None:intergenic 40.0%
CGCCCAGATTGTAAATTTGA+TGG - chr1_3:58637485-58637504 None:intergenic 40.0%
TCAAATTTACAATCTGGGCG+TGG + chr1_3:58637485-58637504 Msa0128790:three_prime_UTR 40.0%
CAAATTTACAATCTGGGCGT+GGG + chr1_3:58637486-58637505 Msa0128790:three_prime_UTR 40.0%
CGAGTATGAGATTAGGGTTT+AGG - chr1_3:58636010-58636029 None:intergenic 40.0%
! AAGCGGGTTTCAGAACTTTA+GGG - chr1_3:58636148-58636167 None:intergenic 40.0%
! AAAGCGGGTTTCAGAACTTT+AGG - chr1_3:58636149-58636168 None:intergenic 40.0%
TTTGGAGGAATAGTGAAAGC+GGG - chr1_3:58636164-58636183 None:intergenic 40.0%
!! AGGGATTGTAACAGTTTTGG+AGG - chr1_3:58636179-58636198 None:intergenic 40.0%
! GCAAGGGATTGTAACAGTTT+TGG - chr1_3:58636182-58636201 None:intergenic 40.0%
! CCATTTAGGATCTTCTGCAA+GGG - chr1_3:58636198-58636217 None:intergenic 40.0%
CCTTGCAGAAGATCCTAAAT+GGG + chr1_3:58636196-58636215 Msa0128790:CDS 40.0%
GATCCTAAATGGGACGATAA+AGG + chr1_3:58636206-58636225 Msa0128790:CDS 40.0%
! GCCGAAAGAGGAATAGTTTT+GGG - chr1_3:58636240-58636259 None:intergenic 40.0%
AGAGAATCAGTAAGGGAGTT+AGG - chr1_3:58636275-58636294 None:intergenic 40.0%
! TGTAGATTTGAAATCGGCGT+TGG + chr1_3:58636453-58636472 Msa0128790:intron 40.0%
! GTAGATTTGAAATCGGCGTT+GGG + chr1_3:58636454-58636473 Msa0128790:intron 40.0%
TTGGGTAAAAGGAGAAGAGA+TGG + chr1_3:58636472-58636491 Msa0128790:CDS 40.0%
AGACGCTGAATTGAAGTCAA+AGG - chr1_3:58636517-58636536 None:intergenic 40.0%
GACTGTCCCAATAAACCTTT+AGG + chr1_3:58636875-58636894 Msa0128790:intron 40.0%
AACGGCCCTAAAGGTTTATT+GGG - chr1_3:58636884-58636903 None:intergenic 40.0%
AAACGGCCCTAAAGGTTTAT+TGG - chr1_3:58636885-58636904 None:intergenic 40.0%
ATTTGCATAAACGGCCCTAA+AGG - chr1_3:58636893-58636912 None:intergenic 40.0%
!! GAAGGTGTACAATTTTCGTC+GGG + chr1_3:58636975-58636994 Msa0128790:intron 40.0%
! AAGGTGTACAATTTTCGTCG+GGG + chr1_3:58636976-58636995 Msa0128790:intron 40.0%
!! TGAGTGGTTGTGATTTGTTG+AGG + chr1_3:58637214-58637233 Msa0128790:intron 40.0%
TTGCAACTGAATGCTGCATA+GGG - chr1_3:58637366-58637385 None:intergenic 40.0%
CGCCCAGATTGTAAATTTGA+TGG - chr1_3:58637485-58637504 None:intergenic 40.0%
TCAAATTTACAATCTGGGCG+TGG + chr1_3:58637485-58637504 Msa0128790:three_prime_UTR 40.0%
CAAATTTACAATCTGGGCGT+GGG + chr1_3:58637486-58637505 Msa0128790:three_prime_UTR 40.0%
GCAACTGCGTAAGCAAGATA+GGG - chr1_3:58636038-58636057 None:intergenic 45.0%
TGCAACTGCGTAAGCAAGAT+AGG - chr1_3:58636039-58636058 None:intergenic 45.0%
GTATAAGCAATCTCGCCCAA+AGG + chr1_3:58636097-58636116 Msa0128790:CDS 45.0%
GAAGACCAACACGTACCTTT+GGG - chr1_3:58636115-58636134 None:intergenic 45.0%
CCCTTGCAGAAGATCCTAAA+TGG + chr1_3:58636195-58636214 Msa0128790:CDS 45.0%
! CCCATTTAGGATCTTCTGCA+AGG - chr1_3:58636199-58636218 None:intergenic 45.0%
GAGCCTTTATCGTCCCATTT+AGG - chr1_3:58636212-58636231 None:intergenic 45.0%
! CGCCGAAAGAGGAATAGTTT+TGG - chr1_3:58636241-58636260 None:intergenic 45.0%
ATCAGAAACAACGCCGAAAG+AGG - chr1_3:58636252-58636271 None:intergenic 45.0%
TCGTTATCATCGGCAACAGA+AGG - chr1_3:58636305-58636324 None:intergenic 45.0%
TCTTCTTCAAGATCACTGCC+GGG - chr1_3:58636329-58636348 None:intergenic 45.0%
GTCTTCTTCAAGATCACTGC+CGG - chr1_3:58636330-58636349 None:intergenic 45.0%
GGCAGTGATCTTGAAGAAGA+CGG + chr1_3:58636329-58636348 Msa0128790:CDS 45.0%
! TGAAATCGGCGTTGGGTAAA+AGG + chr1_3:58636461-58636480 Msa0128790:CDS 45.0%
GTTCTCTTCTAGGTCCTGTT+TGG + chr1_3:58636640-58636659 Msa0128790:intron 45.0%
GTTGCAACTGAATGCTGCAT+AGG - chr1_3:58637367-58637386 None:intergenic 45.0%
GACAAGAATCCGCTCATTGT+TGG + chr1_3:58637424-58637443 Msa0128790:CDS 45.0%
ATGGCATTGCACTTGTCCAT+AGG - chr1_3:58637466-58637485 None:intergenic 45.0%
GCAACTGCGTAAGCAAGATA+GGG - chr1_3:58636038-58636057 None:intergenic 45.0%
TGCAACTGCGTAAGCAAGAT+AGG - chr1_3:58636039-58636058 None:intergenic 45.0%
GTATAAGCAATCTCGCCCAA+AGG + chr1_3:58636097-58636116 Msa0128790:CDS 45.0%
GAAGACCAACACGTACCTTT+GGG - chr1_3:58636115-58636134 None:intergenic 45.0%
CCCTTGCAGAAGATCCTAAA+TGG + chr1_3:58636195-58636214 Msa0128790:CDS 45.0%
! CCCATTTAGGATCTTCTGCA+AGG - chr1_3:58636199-58636218 None:intergenic 45.0%
GAGCCTTTATCGTCCCATTT+AGG - chr1_3:58636212-58636231 None:intergenic 45.0%
! CGCCGAAAGAGGAATAGTTT+TGG - chr1_3:58636241-58636260 None:intergenic 45.0%
ATCAGAAACAACGCCGAAAG+AGG - chr1_3:58636252-58636271 None:intergenic 45.0%
TCGTTATCATCGGCAACAGA+AGG - chr1_3:58636305-58636324 None:intergenic 45.0%
TCTTCTTCAAGATCACTGCC+GGG - chr1_3:58636329-58636348 None:intergenic 45.0%
GTCTTCTTCAAGATCACTGC+CGG - chr1_3:58636330-58636349 None:intergenic 45.0%
GGCAGTGATCTTGAAGAAGA+CGG + chr1_3:58636329-58636348 Msa0128790:CDS 45.0%
! TGAAATCGGCGTTGGGTAAA+AGG + chr1_3:58636461-58636480 Msa0128790:CDS 45.0%
GTTCTCTTCTAGGTCCTGTT+TGG + chr1_3:58636640-58636659 Msa0128790:intron 45.0%
GTTGCAACTGAATGCTGCAT+AGG - chr1_3:58637367-58637386 None:intergenic 45.0%
GACAAGAATCCGCTCATTGT+TGG + chr1_3:58637424-58637443 Msa0128790:CDS 45.0%
ATGGCATTGCACTTGTCCAT+AGG - chr1_3:58637466-58637485 None:intergenic 45.0%
GGAAGACCAACACGTACCTT+TGG - chr1_3:58636116-58636135 None:intergenic 50.0%
! GTTGCCGATGATAACGATCC+CGG + chr1_3:58636308-58636327 Msa0128790:CDS 50.0%
ACTGCCGGGATCGTTATCAT+CGG - chr1_3:58636315-58636334 None:intergenic 50.0%
GGAAGACCAACACGTACCTT+TGG - chr1_3:58636116-58636135 None:intergenic 50.0%
! GTTGCCGATGATAACGATCC+CGG + chr1_3:58636308-58636327 Msa0128790:CDS 50.0%
ACTGCCGGGATCGTTATCAT+CGG - chr1_3:58636315-58636334 None:intergenic 50.0%
! TCTCGCCCAAAGGTACGTGT+TGG + chr1_3:58636107-58636126 Msa0128790:CDS 55.0%
GAGCTGCAACGGTTGATCCA+TGG + chr1_3:58637513-58637532 Msa0128790:three_prime_UTR 55.0%
! TCTCGCCCAAAGGTACGTGT+TGG + chr1_3:58636107-58636126 Msa0128790:CDS 55.0%
GAGCTGCAACGGTTGATCCA+TGG + chr1_3:58637513-58637532 Msa0128790:three_prime_UTR 55.0%
! GCGTGGGTTGTGAGCTGCAA+CGG + chr1_3:58637502-58637521 Msa0128790:three_prime_UTR 60.0%
! GCGTGGGTTGTGAGCTGCAA+CGG + chr1_3:58637502-58637521 Msa0128790:three_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 58635975 58637776 58635975 ID=Msa0128790;Name=Msa0128790
chr1_3 mRNA 58635975 58637776 58635975 ID=Msa0128790-mRNA-1;Parent=Msa0128790;Name=Msa0128790-mRNA-1;_AED=0.01;_eAED=0.01;_QI=102|1|1|1|1|1|3|323|170
chr1_3 exon 58635975 58636350 58635975 ID=Msa0128790-mRNA-1:exon:18841;Parent=Msa0128790-mRNA-1
chr1_3 exon 58636458 58636579 58636458 ID=Msa0128790-mRNA-1:exon:18842;Parent=Msa0128790-mRNA-1
chr1_3 exon 58637337 58637776 58637337 ID=Msa0128790-mRNA-1:exon:18843;Parent=Msa0128790-mRNA-1
chr1_3 five_prime_UTR 58635975 58636076 58635975 ID=Msa0128790-mRNA-1:five_prime_utr;Parent=Msa0128790-mRNA-1
chr1_3 CDS 58636077 58636350 58636077 ID=Msa0128790-mRNA-1:cds;Parent=Msa0128790-mRNA-1
chr1_3 CDS 58636458 58636579 58636458 ID=Msa0128790-mRNA-1:cds;Parent=Msa0128790-mRNA-1
chr1_3 CDS 58637337 58637453 58637337 ID=Msa0128790-mRNA-1:cds;Parent=Msa0128790-mRNA-1
chr1_3 three_prime_UTR 58637454 58637776 58637454 ID=Msa0128790-mRNA-1:three_prime_utr;Parent=Msa0128790-mRNA-1
Gene Sequence

>Msa0128790

ATGGGAAGATCACGAAGAAAGTATAAGCAATCTCGCCCAAAGGTACGTGTTGGTCTTCCAAAGAAGAACCCTAAAGTTCTGAAACCCGCTTTCACTATTCCTCCAAAACTGTTACAATCCCTTGCAGAAGATCCTAAATGGGACGATAAAGGCTCTGTCACCCAAAACTATTCCTCTTTCGGCGTTGTTTCTGATCCTAACTCCCTTACTGATTCTCTTCAAATTCCTTCTGTTGCCGATGATAACGATCCCGGCAGTGATCTTGAAGAAGACGATTTGAAATCGGCGTTGGGTAAAAGGAGAAGAGATGGTAAAAGCTCACTTCCTCAACCTTTGACTTCAATTCAGCGTCTTCATATTAGTCGTTTAGTTGAAAAATATGGAGATGATTTTCAGAAAATGATGATGGATATAAAGCTAAACCCTATGCAGCATTCAGTTGCAACTTTAGAGAAATTGTGCTTCAGCTATTACTTATATGAAGACAAGAATCCGCTCATTGTTGGTAAATGA
Protein sequence

>Msa0128790

MGRSRRKYKQSRPKVRVGLPKKNPKVLKPAFTIPPKLLQSLAEDPKWDDKGSVTQNYSSFGVVSDPNSLTDSLQIPSVADDNDPGSDLEEDDLKSALGKRRRDGKSSLPQPLTSIQRLHISRLVEKYGDDFQKMMMDIKLNPMQHSVATLEKLCFSYYLYEDKNPLIVGK*