AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0082430

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0082430 MtrunA17_Chr1g0195681 99.412 170 1 0 1 170 1 170 7.63e-123 343
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0082430 AT1G02870.1 56.477 193 60 4 1 169 1 193 6.17e-68 205

Find 58 sgRNAs with CRISPR-Local

Find 215 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATAGCTTTCTAAAGTTAAT+TGG 0.136445 1_2:-61292786 Msa0082430:three_prime_UTR
CGAGTATGAGATTAGGGTTT+AGG 0.234991 1_2:+61294249 None:intergenic
AAAGCGGGTTTCAGAACTTT+AGG 0.241847 1_2:+61294110 None:intergenic
CCCTTGCAGAAGATCCTAAA+TGG 0.241943 1_2:-61294061 Msa0082430:CDS
GAGCCTTTATCGTCCCATTT+AGG 0.265744 1_2:+61294047 None:intergenic
TGAAATCGGCGTTGGGTAAA+AGG 0.283106 1_2:-61293795 Msa0082430:CDS
GCAAGGGATTGTAACAGTTT+TGG 0.284108 1_2:+61294077 None:intergenic
CGCCCAGATTGTAAATTTGA+TGG 0.304006 1_2:+61292839 None:intergenic
CGCCGAAAGAGGAATAGTTT+TGG 0.317209 1_2:+61294018 None:intergenic
AGAACTTTAGGGTTCTTCTT+TGG 0.324436 1_2:+61294122 None:intergenic
GAGTATGAGATTAGGGTTTA+GGG 0.339426 1_2:+61294250 None:intergenic
TGGAATGTGATAAGTTGTTT+TGG 0.344430 1_2:-61292573 Msa0082430:three_prime_UTR
ACCCAAAACTATTCCTCTTT+CGG 0.353542 1_2:-61294020 Msa0082430:CDS
GGAAGACCAACACGTACCTT+TGG 0.363935 1_2:+61294143 None:intergenic
ATGCCATCAAATTTACAATC+TGG 0.368534 1_2:-61292842 Msa0082430:three_prime_UTR
TGCAACTGCGTAAGCAAGAT+AGG 0.370438 1_2:+61294220 None:intergenic
AGAGAATCAGTAAGGGAGTT+AGG 0.373552 1_2:+61293984 None:intergenic
GACAAGAATCCGCTCATTGT+TGG 0.413347 1_2:-61292897 Msa0082430:CDS
ATAGCTTTCTAAAGTTAATT+GGG 0.414342 1_2:-61292785 Msa0082430:three_prime_UTR
AAGCGGGTTTCAGAACTTTA+GGG 0.416206 1_2:+61294111 None:intergenic
GAAGACCAACACGTACCTTT+GGG 0.427653 1_2:+61294144 None:intergenic
GATCCTAAATGGGACGATAA+AGG 0.428418 1_2:-61294050 Msa0082430:CDS
TTGTGGTGTAGATTTGAAAT+CGG 0.469021 1_2:-61293809 Msa0082430:intron
GACACAGAGAGAAGAAGAAT+GGG 0.476563 1_2:-61294197 Msa0082430:exon
GAATTTGAAGAGAATCAGTA+AGG 0.477015 1_2:+61293976 None:intergenic
TGCCATCAAATTTACAATCT+GGG 0.479882 1_2:-61292841 Msa0082430:three_prime_UTR
GTCTTCTTCAAGATCACTGC+CGG 0.487579 1_2:+61293929 None:intergenic
GTAGATTTGAAATCGGCGTT+GGG 0.505626 1_2:-61293802 Msa0082430:intron
AGACACAGAGAGAAGAAGAA+TGG 0.507644 1_2:-61294198 Msa0082430:exon
TCCATGCAAAATTCAAAAGC+AGG 0.510757 1_2:+61292673 None:intergenic
TCAAATTTACAATCTGGGCG+TGG 0.510966 1_2:-61292836 Msa0082430:three_prime_UTR
ATGGCATTGCACTTGTCCAT+AGG 0.520593 1_2:+61292858 None:intergenic
TGAATTGAAGTCAAAGGTTG+AGG 0.526816 1_2:+61293748 None:intergenic
TGTAGATTTGAAATCGGCGT+TGG 0.527237 1_2:-61293803 Msa0082430:intron
ATCAGAAACAACGCCGAAAG+AGG 0.531608 1_2:+61294007 None:intergenic
GCGTGGGTTGTGAGCTGCAA+CGG 0.531681 1_2:-61292819 Msa0082430:three_prime_UTR
GTTGCAACTGAATGCTGCAT+AGG 0.533068 1_2:+61292957 None:intergenic
TTGCAACTGAATGCTGCATA+GGG 0.539277 1_2:+61292958 None:intergenic
CCTTGCAGAAGATCCTAAAT+GGG 0.546570 1_2:-61294060 Msa0082430:CDS
CCATTTAGGATCTTCTGCAA+GGG 0.547386 1_2:+61294061 None:intergenic
TAACTTTAGAAAGCTATCCA+TGG 0.552988 1_2:+61292791 None:intergenic
CCCATTTAGGATCTTCTGCA+AGG 0.554925 1_2:+61294060 None:intergenic
GTTGCCGATGATAACGATCC+CGG 0.559862 1_2:-61293948 Msa0082430:CDS
CAAATTTACAATCTGGGCGT+GGG 0.560830 1_2:-61292835 Msa0082430:three_prime_UTR
TTTGGAGGAATAGTGAAAGC+GGG 0.577554 1_2:+61294095 None:intergenic
TCTTCTTCAAGATCACTGCC+GGG 0.597126 1_2:+61293930 None:intergenic
TCGTTATCATCGGCAACAGA+AGG 0.598477 1_2:+61293954 None:intergenic
GCAACTGCGTAAGCAAGATA+GGG 0.606004 1_2:+61294221 None:intergenic
AGACGCTGAATTGAAGTCAA+AGG 0.608242 1_2:+61293742 None:intergenic
GAGCTGCAACGGTTGATCCA+TGG 0.610724 1_2:-61292808 Msa0082430:three_prime_UTR
AATTTGAAGAGAATCAGTAA+GGG 0.613175 1_2:+61293977 None:intergenic
TTGGGTAAAAGGAGAAGAGA+TGG 0.616129 1_2:-61293784 Msa0082430:CDS
GGCAGTGATCTTGAAGAAGA+CGG 0.629513 1_2:-61293927 Msa0082430:intron
TCTCGCCCAAAGGTACGTGT+TGG 0.632503 1_2:-61294149 Msa0082430:CDS
GTATAAGCAATCTCGCCCAA+AGG 0.632811 1_2:-61294159 Msa0082430:CDS
TCTCATTTACCAACAATGAG+CGG 0.654335 1_2:+61292888 None:intergenic
AGCAAAATGTTGTTGCAATG+AGG 0.658596 1_2:-61292755 Msa0082430:three_prime_UTR
ACTGCCGGGATCGTTATCAT+CGG 0.664050 1_2:+61293944 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTGTTTTGTTTTTATTT+TGG - chr1_2:61293843-61293862 Msa0082430:intron 10.0%
!!! TTATTGTTTTGTTTTTATTT+TGG - chr1_2:61293843-61293862 Msa0082430:intron 10.0%
!!! TTAAAACTTTTGAAAATGAA+CGG - chr1_2:61293381-61293400 Msa0082430:intron 15.0%
!! AACTAATTTATTTGCATAAA+CGG + chr1_2:61293505-61293524 None:intergenic 15.0%
!!! ACTTTCATCTATTAATTTTT+TGG + chr1_2:61294252-61294271 None:intergenic 15.0%
!!! TTAAAACTTTTGAAAATGAA+CGG - chr1_2:61293381-61293400 Msa0082430:intron 15.0%
!! AACTAATTTATTTGCATAAA+CGG + chr1_2:61293505-61293524 None:intergenic 15.0%
!!! ACTTTCATCTATTAATTTTT+TGG + chr1_2:61294252-61294271 None:intergenic 15.0%
!! GTTGAAATTAGTATTAATCT+TGG - chr1_2:61292985-61293004 Msa0082430:CDS 20.0%
!! GAATGTAAATAATTTGAGTA+TGG - chr1_2:61293019-61293038 Msa0082430:intron 20.0%
!!! TGTTTTTTCATAAATTACCT+TGG + chr1_2:61293551-61293570 None:intergenic 20.0%
!!! ATAGCTTTCTAAAGTTAATT+GGG - chr1_2:61294068-61294087 Msa0082430:CDS 20.0%
!! GTTGAAATTAGTATTAATCT+TGG - chr1_2:61292985-61293004 Msa0082430:CDS 20.0%
!! GAATGTAAATAATTTGAGTA+TGG - chr1_2:61293019-61293038 Msa0082430:intron 20.0%
!!! TGTTTTTTCATAAATTACCT+TGG + chr1_2:61293551-61293570 None:intergenic 20.0%
!!! ATAGCTTTCTAAAGTTAATT+GGG - chr1_2:61294068-61294087 Msa0082430:CDS 20.0%
! AATTTGAAGAGAATCAGTAA+GGG + chr1_2:61292879-61292898 None:intergenic 25.0%
! TAACAATTTACGATGAATTG+AGG + chr1_2:61292958-61292977 None:intergenic 25.0%
!! ATTAATCTTGGTAATTCTGA+AGG - chr1_2:61292997-61293016 Msa0082430:intron 25.0%
!!! ATAATTTGAGTATGGTTTTG+TGG - chr1_2:61293027-61293046 Msa0082430:intron 25.0%
! AGTCGTTTAGTTGAAAAATA+TGG - chr1_2:61293141-61293160 Msa0082430:intron 25.0%
!! AAAATATGGAGATGATTTTC+AGG - chr1_2:61293155-61293174 Msa0082430:intron 25.0%
!!! GATAATTTGTTTATCCAAAC+AGG + chr1_2:61293254-61293273 None:intergenic 25.0%
!!! TTTATCTTTTGCTATAGAAG+TGG + chr1_2:61293321-61293340 None:intergenic 25.0%
!!! ATAAGTTTTGTGAGATTGTT+TGG + chr1_2:61293436-61293455 None:intergenic 25.0%
! TATGAAAAAACAGCTTATGA+AGG - chr1_2:61293559-61293578 Msa0082430:intron 25.0%
!! CATTTTACTCATATACTACA+TGG - chr1_2:61293635-61293654 Msa0082430:intron 25.0%
! GTATCATAATCTTGAAACAT+CGG - chr1_2:61293691-61293710 Msa0082430:intron 25.0%
!!! GATAGCTTTCTAAAGTTAAT+TGG - chr1_2:61294067-61294086 Msa0082430:CDS 25.0%
!! AATTTTGCATGGATTTTTGT+TGG - chr1_2:61294190-61294209 Msa0082430:exon 25.0%
!! ATTTTGCATGGATTTTTGTT+GGG - chr1_2:61294191-61294210 Msa0082430:exon 25.0%
!!! TTTTTTGGCAACAACAATTT+GGG + chr1_2:61294237-61294256 None:intergenic 25.0%
!!! ATTTTTTGGCAACAACAATT+TGG + chr1_2:61294238-61294257 None:intergenic 25.0%
!!! ATAGATGAAAGTAGTTTTGA+TGG - chr1_2:61294260-61294279 Msa0082430:five_prime_UTR 25.0%
! AATTTGAAGAGAATCAGTAA+GGG + chr1_2:61292879-61292898 None:intergenic 25.0%
! TAACAATTTACGATGAATTG+AGG + chr1_2:61292958-61292977 None:intergenic 25.0%
!! ATTAATCTTGGTAATTCTGA+AGG - chr1_2:61292997-61293016 Msa0082430:intron 25.0%
!!! ATAATTTGAGTATGGTTTTG+TGG - chr1_2:61293027-61293046 Msa0082430:intron 25.0%
! AGTCGTTTAGTTGAAAAATA+TGG - chr1_2:61293141-61293160 Msa0082430:intron 25.0%
!! AAAATATGGAGATGATTTTC+AGG - chr1_2:61293155-61293174 Msa0082430:intron 25.0%
!!! GATAATTTGTTTATCCAAAC+AGG + chr1_2:61293254-61293273 None:intergenic 25.0%
!!! TTTATCTTTTGCTATAGAAG+TGG + chr1_2:61293321-61293340 None:intergenic 25.0%
!!! ATAAGTTTTGTGAGATTGTT+TGG + chr1_2:61293436-61293455 None:intergenic 25.0%
! TATGAAAAAACAGCTTATGA+AGG - chr1_2:61293559-61293578 Msa0082430:intron 25.0%
!! CATTTTACTCATATACTACA+TGG - chr1_2:61293635-61293654 Msa0082430:intron 25.0%
! GTATCATAATCTTGAAACAT+CGG - chr1_2:61293691-61293710 Msa0082430:intron 25.0%
!!! GATAGCTTTCTAAAGTTAAT+TGG - chr1_2:61294067-61294086 Msa0082430:CDS 25.0%
!! AATTTTGCATGGATTTTTGT+TGG - chr1_2:61294190-61294209 Msa0082430:exon 25.0%
!! ATTTTGCATGGATTTTTGTT+GGG - chr1_2:61294191-61294210 Msa0082430:exon 25.0%
!!! TTTTTTGGCAACAACAATTT+GGG + chr1_2:61294237-61294256 None:intergenic 25.0%
!!! ATTTTTTGGCAACAACAATT+TGG + chr1_2:61294238-61294257 None:intergenic 25.0%
!!! ATAGATGAAAGTAGTTTTGA+TGG - chr1_2:61294260-61294279 Msa0082430:five_prime_UTR 25.0%
!!! GTTTTTCGAGTATGAGATTA+GGG + chr1_2:61292613-61292632 None:intergenic 30.0%
GAATTTGAAGAGAATCAGTA+AGG + chr1_2:61292880-61292899 None:intergenic 30.0%
! TTGTGGTGTAGATTTGAAAT+CGG - chr1_2:61293044-61293063 Msa0082430:intron 30.0%
TATTATTCATAAGCTTGCCA+AGG - chr1_2:61293531-61293550 Msa0082430:intron 30.0%
!! TATGAAGGTACAATTTTCGT+CGG - chr1_2:61293574-61293593 Msa0082430:intron 30.0%
TACTCATATACTACATGGTT+AGG - chr1_2:61293640-61293659 Msa0082430:intron 30.0%
! TCTAAAGTAACACAAATGAG+TGG - chr1_2:61293730-61293749 Msa0082430:CDS 30.0%
! CAAATGTTACTCTGTTTTCT+TGG - chr1_2:61293794-61293813 Msa0082430:CDS 30.0%
!! TACTCTGTTTTCTTGGTTTT+AGG - chr1_2:61293801-61293820 Msa0082430:CDS 30.0%
!!! GTTTTAGGACTTCATTTATG+TGG - chr1_2:61293816-61293835 Msa0082430:intron 30.0%
!! ATTTTGGCAGAAAATGATGA+TGG - chr1_2:61293859-61293878 Msa0082430:intron 30.0%
! TAAATGAGATGTTTTGCCTA+TGG - chr1_2:61293979-61293998 Msa0082430:CDS 30.0%
ATGCCATCAAATTTACAATC+TGG - chr1_2:61294011-61294030 Msa0082430:CDS 30.0%
TGCCATCAAATTTACAATCT+GGG - chr1_2:61294012-61294031 Msa0082430:CDS 30.0%
! TAACTTTAGAAAGCTATCCA+TGG + chr1_2:61294065-61294084 None:intergenic 30.0%
! TGGAATGTGATAAGTTGTTT+TGG - chr1_2:61294280-61294299 Msa0082430:five_prime_UTR 30.0%
!! GGAATGTGATAAGTTGTTTT+GGG - chr1_2:61294281-61294300 Msa0082430:five_prime_UTR 30.0%
!!! GTTTTTCGAGTATGAGATTA+GGG + chr1_2:61292613-61292632 None:intergenic 30.0%
GAATTTGAAGAGAATCAGTA+AGG + chr1_2:61292880-61292899 None:intergenic 30.0%
! TTGTGGTGTAGATTTGAAAT+CGG - chr1_2:61293044-61293063 Msa0082430:intron 30.0%
TATTATTCATAAGCTTGCCA+AGG - chr1_2:61293531-61293550 Msa0082430:intron 30.0%
!! TATGAAGGTACAATTTTCGT+CGG - chr1_2:61293574-61293593 Msa0082430:intron 30.0%
TACTCATATACTACATGGTT+AGG - chr1_2:61293640-61293659 Msa0082430:intron 30.0%
! TCTAAAGTAACACAAATGAG+TGG - chr1_2:61293730-61293749 Msa0082430:CDS 30.0%
! CAAATGTTACTCTGTTTTCT+TGG - chr1_2:61293794-61293813 Msa0082430:CDS 30.0%
!! TACTCTGTTTTCTTGGTTTT+AGG - chr1_2:61293801-61293820 Msa0082430:CDS 30.0%
!!! GTTTTAGGACTTCATTTATG+TGG - chr1_2:61293816-61293835 Msa0082430:intron 30.0%
!! ATTTTGGCAGAAAATGATGA+TGG - chr1_2:61293859-61293878 Msa0082430:intron 30.0%
! TAAATGAGATGTTTTGCCTA+TGG - chr1_2:61293979-61293998 Msa0082430:CDS 30.0%
ATGCCATCAAATTTACAATC+TGG - chr1_2:61294011-61294030 Msa0082430:CDS 30.0%
TGCCATCAAATTTACAATCT+GGG - chr1_2:61294012-61294031 Msa0082430:CDS 30.0%
! TAACTTTAGAAAGCTATCCA+TGG + chr1_2:61294065-61294084 None:intergenic 30.0%
! TGGAATGTGATAAGTTGTTT+TGG - chr1_2:61294280-61294299 Msa0082430:five_prime_UTR 30.0%
GAGTATGAGATTAGGGTTTA+GGG + chr1_2:61292606-61292625 None:intergenic 35.0%
!!! GGTTTTTCGAGTATGAGATT+AGG + chr1_2:61292614-61292633 None:intergenic 35.0%
!! AGAACTTTAGGGTTCTTCTT+TGG + chr1_2:61292734-61292753 None:intergenic 35.0%
!! TTTTGGAGGAATAGTGAAAG+CGG + chr1_2:61292762-61292781 None:intergenic 35.0%
ACCCAAAACTATTCCTCTTT+CGG - chr1_2:61292833-61292852 Msa0082430:three_prime_UTR 35.0%
TGAATTGAAGTCAAAGGTTG+AGG + chr1_2:61293108-61293127 None:intergenic 35.0%
!!! CGTTTTTTTCACTGTTGCTT+TGG - chr1_2:61293180-61293199 Msa0082430:intron 35.0%
! AGCTTTGATAGTTCACTTCT+AGG - chr1_2:61293227-61293246 Msa0082430:intron 35.0%
TCTCACAAAACTTATGTCAG+TGG - chr1_2:61293441-61293460 Msa0082430:intron 35.0%
TCTCATTTACCAACAATGAG+CGG + chr1_2:61293968-61293987 None:intergenic 35.0%
AGCAAAATGTTGTTGCAATG+AGG - chr1_2:61294098-61294117 Msa0082430:CDS 35.0%
!! TCCTGCTTTTGAATTTTGCA+TGG - chr1_2:61294179-61294198 Msa0082430:CDS 35.0%
TCCATGCAAAATTCAAAAGC+AGG + chr1_2:61294183-61294202 None:intergenic 35.0%
GAGTATGAGATTAGGGTTTA+GGG + chr1_2:61292606-61292625 None:intergenic 35.0%
!!! GGTTTTTCGAGTATGAGATT+AGG + chr1_2:61292614-61292633 None:intergenic 35.0%
!! AGAACTTTAGGGTTCTTCTT+TGG + chr1_2:61292734-61292753 None:intergenic 35.0%
!! TTTTGGAGGAATAGTGAAAG+CGG + chr1_2:61292762-61292781 None:intergenic 35.0%
ACCCAAAACTATTCCTCTTT+CGG - chr1_2:61292833-61292852 Msa0082430:three_prime_UTR 35.0%
TGAATTGAAGTCAAAGGTTG+AGG + chr1_2:61293108-61293127 None:intergenic 35.0%
!!! CGTTTTTTTCACTGTTGCTT+TGG - chr1_2:61293180-61293199 Msa0082430:intron 35.0%
! AGCTTTGATAGTTCACTTCT+AGG - chr1_2:61293227-61293246 Msa0082430:intron 35.0%
TCTCACAAAACTTATGTCAG+TGG - chr1_2:61293441-61293460 Msa0082430:intron 35.0%
TCTCATTTACCAACAATGAG+CGG + chr1_2:61293968-61293987 None:intergenic 35.0%
AGCAAAATGTTGTTGCAATG+AGG - chr1_2:61294098-61294117 Msa0082430:CDS 35.0%
!! TCCTGCTTTTGAATTTTGCA+TGG - chr1_2:61294179-61294198 Msa0082430:CDS 35.0%
TCCATGCAAAATTCAAAAGC+AGG + chr1_2:61294183-61294202 None:intergenic 35.0%
CGAGTATGAGATTAGGGTTT+AGG + chr1_2:61292607-61292626 None:intergenic 40.0%
AGACACAGAGAGAAGAAGAA+TGG - chr1_2:61292655-61292674 Msa0082430:three_prime_UTR 40.0%
GACACAGAGAGAAGAAGAAT+GGG - chr1_2:61292656-61292675 Msa0082430:three_prime_UTR 40.0%
! AAGCGGGTTTCAGAACTTTA+GGG + chr1_2:61292745-61292764 None:intergenic 40.0%
! AAAGCGGGTTTCAGAACTTT+AGG + chr1_2:61292746-61292765 None:intergenic 40.0%
TTTGGAGGAATAGTGAAAGC+GGG + chr1_2:61292761-61292780 None:intergenic 40.0%
!! AGGGATTGTAACAGTTTTGG+AGG + chr1_2:61292776-61292795 None:intergenic 40.0%
! GCAAGGGATTGTAACAGTTT+TGG + chr1_2:61292779-61292798 None:intergenic 40.0%
! CCATTTAGGATCTTCTGCAA+GGG + chr1_2:61292795-61292814 None:intergenic 40.0%
CCTTGCAGAAGATCCTAAAT+GGG - chr1_2:61292793-61292812 Msa0082430:three_prime_UTR 40.0%
GATCCTAAATGGGACGATAA+AGG - chr1_2:61292803-61292822 Msa0082430:three_prime_UTR 40.0%
! GCCGAAAGAGGAATAGTTTT+GGG + chr1_2:61292837-61292856 None:intergenic 40.0%
AGAGAATCAGTAAGGGAGTT+AGG + chr1_2:61292872-61292891 None:intergenic 40.0%
! TGTAGATTTGAAATCGGCGT+TGG - chr1_2:61293050-61293069 Msa0082430:intron 40.0%
! GTAGATTTGAAATCGGCGTT+GGG - chr1_2:61293051-61293070 Msa0082430:intron 40.0%
TTGGGTAAAAGGAGAAGAGA+TGG - chr1_2:61293069-61293088 Msa0082430:intron 40.0%
AGACGCTGAATTGAAGTCAA+AGG + chr1_2:61293114-61293133 None:intergenic 40.0%
CCTGTCCCAATAAACCTTTA+GGG - chr1_2:61293479-61293498 Msa0082430:intron 40.0%
AACGGCCCTAAAGGTTTATT+GGG + chr1_2:61293487-61293506 None:intergenic 40.0%
AAACGGCCCTAAAGGTTTAT+TGG + chr1_2:61293488-61293507 None:intergenic 40.0%
ATTTGCATAAACGGCCCTAA+AGG + chr1_2:61293496-61293515 None:intergenic 40.0%
!! TGAGTGGTTGTGATTTGTTG+AGG - chr1_2:61293746-61293765 Msa0082430:CDS 40.0%
TTGCAACTGAATGCTGCATA+GGG + chr1_2:61293898-61293917 None:intergenic 40.0%
CGCCCAGATTGTAAATTTGA+TGG + chr1_2:61294017-61294036 None:intergenic 40.0%
TCAAATTTACAATCTGGGCG+TGG - chr1_2:61294017-61294036 Msa0082430:CDS 40.0%
CAAATTTACAATCTGGGCGT+GGG - chr1_2:61294018-61294037 Msa0082430:CDS 40.0%
CGAGTATGAGATTAGGGTTT+AGG + chr1_2:61292607-61292626 None:intergenic 40.0%
AGACACAGAGAGAAGAAGAA+TGG - chr1_2:61292655-61292674 Msa0082430:three_prime_UTR 40.0%
GACACAGAGAGAAGAAGAAT+GGG - chr1_2:61292656-61292675 Msa0082430:three_prime_UTR 40.0%
! AAGCGGGTTTCAGAACTTTA+GGG + chr1_2:61292745-61292764 None:intergenic 40.0%
! AAAGCGGGTTTCAGAACTTT+AGG + chr1_2:61292746-61292765 None:intergenic 40.0%
TTTGGAGGAATAGTGAAAGC+GGG + chr1_2:61292761-61292780 None:intergenic 40.0%
!! AGGGATTGTAACAGTTTTGG+AGG + chr1_2:61292776-61292795 None:intergenic 40.0%
! GCAAGGGATTGTAACAGTTT+TGG + chr1_2:61292779-61292798 None:intergenic 40.0%
! CCATTTAGGATCTTCTGCAA+GGG + chr1_2:61292795-61292814 None:intergenic 40.0%
CCTTGCAGAAGATCCTAAAT+GGG - chr1_2:61292793-61292812 Msa0082430:three_prime_UTR 40.0%
GATCCTAAATGGGACGATAA+AGG - chr1_2:61292803-61292822 Msa0082430:three_prime_UTR 40.0%
! GCCGAAAGAGGAATAGTTTT+GGG + chr1_2:61292837-61292856 None:intergenic 40.0%
AGAGAATCAGTAAGGGAGTT+AGG + chr1_2:61292872-61292891 None:intergenic 40.0%
! TGTAGATTTGAAATCGGCGT+TGG - chr1_2:61293050-61293069 Msa0082430:intron 40.0%
! GTAGATTTGAAATCGGCGTT+GGG - chr1_2:61293051-61293070 Msa0082430:intron 40.0%
TTGGGTAAAAGGAGAAGAGA+TGG - chr1_2:61293069-61293088 Msa0082430:intron 40.0%
AGACGCTGAATTGAAGTCAA+AGG + chr1_2:61293114-61293133 None:intergenic 40.0%
CCTGTCCCAATAAACCTTTA+GGG - chr1_2:61293479-61293498 Msa0082430:intron 40.0%
AACGGCCCTAAAGGTTTATT+GGG + chr1_2:61293487-61293506 None:intergenic 40.0%
AAACGGCCCTAAAGGTTTAT+TGG + chr1_2:61293488-61293507 None:intergenic 40.0%
ATTTGCATAAACGGCCCTAA+AGG + chr1_2:61293496-61293515 None:intergenic 40.0%
!! TGAGTGGTTGTGATTTGTTG+AGG - chr1_2:61293746-61293765 Msa0082430:CDS 40.0%
TTGCAACTGAATGCTGCATA+GGG + chr1_2:61293898-61293917 None:intergenic 40.0%
CGCCCAGATTGTAAATTTGA+TGG + chr1_2:61294017-61294036 None:intergenic 40.0%
TCAAATTTACAATCTGGGCG+TGG - chr1_2:61294017-61294036 Msa0082430:CDS 40.0%
CAAATTTACAATCTGGGCGT+GGG - chr1_2:61294018-61294037 Msa0082430:CDS 40.0%
GCAACTGCGTAAGCAAGATA+GGG + chr1_2:61292635-61292654 None:intergenic 45.0%
TGCAACTGCGTAAGCAAGAT+AGG + chr1_2:61292636-61292655 None:intergenic 45.0%
GTATAAGCAATCTCGCCCAA+AGG - chr1_2:61292694-61292713 Msa0082430:three_prime_UTR 45.0%
GAAGACCAACACGTACCTTT+GGG + chr1_2:61292712-61292731 None:intergenic 45.0%
CCCTTGCAGAAGATCCTAAA+TGG - chr1_2:61292792-61292811 Msa0082430:three_prime_UTR 45.0%
! CCCATTTAGGATCTTCTGCA+AGG + chr1_2:61292796-61292815 None:intergenic 45.0%
GAGCCTTTATCGTCCCATTT+AGG + chr1_2:61292809-61292828 None:intergenic 45.0%
! CGCCGAAAGAGGAATAGTTT+TGG + chr1_2:61292838-61292857 None:intergenic 45.0%
ATCAGAAACAACGCCGAAAG+AGG + chr1_2:61292849-61292868 None:intergenic 45.0%
TCGTTATCATCGGCAACAGA+AGG + chr1_2:61292902-61292921 None:intergenic 45.0%
TCTTCTTCAAGATCACTGCC+GGG + chr1_2:61292926-61292945 None:intergenic 45.0%
GTCTTCTTCAAGATCACTGC+CGG + chr1_2:61292927-61292946 None:intergenic 45.0%
GGCAGTGATCTTGAAGAAGA+CGG - chr1_2:61292926-61292945 Msa0082430:CDS 45.0%
! TGAAATCGGCGTTGGGTAAA+AGG - chr1_2:61293058-61293077 Msa0082430:intron 45.0%
GTTCACTTCTAGGTCCTGTT+TGG - chr1_2:61293237-61293256 Msa0082430:intron 45.0%
GCCTGTCCCAATAAACCTTT+AGG - chr1_2:61293478-61293497 Msa0082430:intron 45.0%
CCCTAAAGGTTTATTGGGAC+AGG + chr1_2:61293482-61293501 None:intergenic 45.0%
GTTGCAACTGAATGCTGCAT+AGG + chr1_2:61293899-61293918 None:intergenic 45.0%
GACAAGAATCCGCTCATTGT+TGG - chr1_2:61293956-61293975 Msa0082430:CDS 45.0%
ATGGCATTGCACTTGTCCAT+AGG + chr1_2:61293998-61294017 None:intergenic 45.0%
GCAACTGCGTAAGCAAGATA+GGG + chr1_2:61292635-61292654 None:intergenic 45.0%
TGCAACTGCGTAAGCAAGAT+AGG + chr1_2:61292636-61292655 None:intergenic 45.0%
GTATAAGCAATCTCGCCCAA+AGG - chr1_2:61292694-61292713 Msa0082430:three_prime_UTR 45.0%
GAAGACCAACACGTACCTTT+GGG + chr1_2:61292712-61292731 None:intergenic 45.0%
CCCTTGCAGAAGATCCTAAA+TGG - chr1_2:61292792-61292811 Msa0082430:three_prime_UTR 45.0%
! CCCATTTAGGATCTTCTGCA+AGG + chr1_2:61292796-61292815 None:intergenic 45.0%
GAGCCTTTATCGTCCCATTT+AGG + chr1_2:61292809-61292828 None:intergenic 45.0%
! CGCCGAAAGAGGAATAGTTT+TGG + chr1_2:61292838-61292857 None:intergenic 45.0%
ATCAGAAACAACGCCGAAAG+AGG + chr1_2:61292849-61292868 None:intergenic 45.0%
TCGTTATCATCGGCAACAGA+AGG + chr1_2:61292902-61292921 None:intergenic 45.0%
TCTTCTTCAAGATCACTGCC+GGG + chr1_2:61292926-61292945 None:intergenic 45.0%
GTCTTCTTCAAGATCACTGC+CGG + chr1_2:61292927-61292946 None:intergenic 45.0%
GGCAGTGATCTTGAAGAAGA+CGG - chr1_2:61292926-61292945 Msa0082430:CDS 45.0%
! TGAAATCGGCGTTGGGTAAA+AGG - chr1_2:61293058-61293077 Msa0082430:intron 45.0%
GTTCACTTCTAGGTCCTGTT+TGG - chr1_2:61293237-61293256 Msa0082430:intron 45.0%
GCCTGTCCCAATAAACCTTT+AGG - chr1_2:61293478-61293497 Msa0082430:intron 45.0%
CCCTAAAGGTTTATTGGGAC+AGG + chr1_2:61293482-61293501 None:intergenic 45.0%
GTTGCAACTGAATGCTGCAT+AGG + chr1_2:61293899-61293918 None:intergenic 45.0%
GACAAGAATCCGCTCATTGT+TGG - chr1_2:61293956-61293975 Msa0082430:CDS 45.0%
ATGGCATTGCACTTGTCCAT+AGG + chr1_2:61293998-61294017 None:intergenic 45.0%
GGAAGACCAACACGTACCTT+TGG + chr1_2:61292713-61292732 None:intergenic 50.0%
! GTTGCCGATGATAACGATCC+CGG - chr1_2:61292905-61292924 Msa0082430:CDS 50.0%
ACTGCCGGGATCGTTATCAT+CGG + chr1_2:61292912-61292931 None:intergenic 50.0%
GGAAGACCAACACGTACCTT+TGG + chr1_2:61292713-61292732 None:intergenic 50.0%
! GTTGCCGATGATAACGATCC+CGG - chr1_2:61292905-61292924 Msa0082430:CDS 50.0%
ACTGCCGGGATCGTTATCAT+CGG + chr1_2:61292912-61292931 None:intergenic 50.0%
! TCTCGCCCAAAGGTACGTGT+TGG - chr1_2:61292704-61292723 Msa0082430:three_prime_UTR 55.0%
GAGCTGCAACGGTTGATCCA+TGG - chr1_2:61294045-61294064 Msa0082430:CDS 55.0%
! TCTCGCCCAAAGGTACGTGT+TGG - chr1_2:61292704-61292723 Msa0082430:three_prime_UTR 55.0%
GAGCTGCAACGGTTGATCCA+TGG - chr1_2:61294045-61294064 Msa0082430:CDS 55.0%
! GCGTGGGTTGTGAGCTGCAA+CGG - chr1_2:61294034-61294053 Msa0082430:CDS 60.0%
! GCGTGGGTTGTGAGCTGCAA+CGG - chr1_2:61294034-61294053 Msa0082430:CDS 60.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 61292572 61294303 61292572 ID=Msa0082430;Name=Msa0082430
chr1_2 mRNA 61292572 61294303 61292572 ID=Msa0082430-mRNA-1;Parent=Msa0082430;Name=Msa0082430-mRNA-1;_AED=0.03;_eAED=0.03;_QI=102|1|1|1|1|1|3|318|170
chr1_2 exon 61292572 61293006 61292572 ID=Msa0082430-mRNA-1:exon:20302;Parent=Msa0082430-mRNA-1
chr1_2 exon 61293699 61293820 61293699 ID=Msa0082430-mRNA-1:exon:20301;Parent=Msa0082430-mRNA-1
chr1_2 exon 61293928 61294303 61293928 ID=Msa0082430-mRNA-1:exon:20300;Parent=Msa0082430-mRNA-1
chr1_2 five_prime_UTR 61294202 61294303 61294202 ID=Msa0082430-mRNA-1:five_prime_utr;Parent=Msa0082430-mRNA-1
chr1_2 CDS 61293928 61294201 61293928 ID=Msa0082430-mRNA-1:cds;Parent=Msa0082430-mRNA-1
chr1_2 CDS 61293699 61293820 61293699 ID=Msa0082430-mRNA-1:cds;Parent=Msa0082430-mRNA-1
chr1_2 CDS 61292890 61293006 61292890 ID=Msa0082430-mRNA-1:cds;Parent=Msa0082430-mRNA-1
chr1_2 three_prime_UTR 61292572 61292889 61292572 ID=Msa0082430-mRNA-1:three_prime_utr;Parent=Msa0082430-mRNA-1
Gene Sequence

>Msa0082430

ATGGGAAGATCACGAAGAAAGTATAAGCAATCTCGCCCAAAGGTACGTGTTGGTCTTCCAAAGAAGAACCCTAAAGTTCTGAAACCCGCTTTCACTATTCCTCCAAAACTGTTACAATCCCTTGCAGAAGATCCTAAATGGGACGATAAAGGCTCTGTCACCCAAAACTATTCCTCTTTCGGCGTTGTTTCTGATCCTAACTCCCTTACTGATTCTCTTCAAATTCCTTCTGTTGCCGATGATAACGATCCCGGCAGTGATCTTGAAGAAGACGATTTGAAATCGGCGTTGGGTAAAAGGAGAAGAGATGGTAAAAGCTCACTTCCTCAACCTTTGACTTCAATTCAGCGTCTTCATATTAGTCGTTTAGTTGAAAAATATGGAGATGATTTTCAGAAAATGATGATGGATATAAAGCTAAACCCTATGCAGCATTCAGTTGCAACTTTAGAGAAATTGTGCTTCAGCTATTACTTATATAAAGACAAGAATCCGCTCATTGTTGGTAAATGA
Protein sequence

>Msa0082430

MGRSRRKYKQSRPKVRVGLPKKNPKVLKPAFTIPPKLLQSLAEDPKWDDKGSVTQNYSSFGVVSDPNSLTDSLQIPSVADDNDPGSDLEEDDLKSALGKRRRDGKSSLPQPLTSIQRLHISRLVEKYGDDFQKMMMDIKLNPMQHSVATLEKLCFSYYLYKDKNPLIVGK*