AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0128990

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0128990 MtrunA17_Chr1g0195681 99.412 170 1 0 1 170 1 170 7.63e-123 343
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0128990 AT1G02870.1 56.477 193 60 4 1 169 1 193 6.17e-68 205

Find 60 sgRNAs with CRISPR-Local

Find 227 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATAGCTTTCTAAAGTTAAT+TGG 0.136445 1_3:-58825914 Msa0128990:three_prime_UTR
CGAGTATGAGATTAGGGTTT+AGG 0.234991 1_3:+58827440 None:intergenic
AAAGCGGGTTTCAGAACTTT+AGG 0.241847 1_3:+58827301 None:intergenic
CCCTTGCAGAAGATCCTAAA+TGG 0.241943 1_3:-58827252 Msa0128990:CDS
TTAATAGATGAAAGTAGTTT+TGG 0.244162 1_3:-58825722 Msa0128990:three_prime_UTR
GAGCCTTTATCGTCCCATTT+AGG 0.265744 1_3:+58827238 None:intergenic
TGAAATCGGCGTTGGGTAAA+AGG 0.283106 1_3:-58826986 Msa0128990:CDS
GCAAGGGATTGTAACAGTTT+TGG 0.284108 1_3:+58827268 None:intergenic
CGCCCAGATTGTAAATTTGA+TGG 0.304006 1_3:+58825967 None:intergenic
CGCCGAAAGAGGAATAGTTT+TGG 0.317209 1_3:+58827209 None:intergenic
AGAACTTTAGGGTTCTTCTT+TGG 0.324436 1_3:+58827313 None:intergenic
TTGGGAAAGATGTAGTATTC+TGG 0.325763 1_3:-58825772 Msa0128990:three_prime_UTR
TGGAATGTGATAAGTTGTTT+TGG 0.328418 1_3:-58825698 Msa0128990:three_prime_UTR
GAGTATGAGATTAGGGTTTA+GGG 0.339426 1_3:+58827441 None:intergenic
ACCCAAAACTATTCCTCTTT+CGG 0.353542 1_3:-58827211 Msa0128990:CDS
GGAAGACCAACACGTACCTT+TGG 0.363935 1_3:+58827334 None:intergenic
ATGCCATCAAATTTACAATC+TGG 0.368534 1_3:-58825970 Msa0128990:three_prime_UTR
TGCAACTGCGTAAGCAAGAT+AGG 0.370438 1_3:+58827411 None:intergenic
AGAGAATCAGTAAGGGAGTT+AGG 0.373552 1_3:+58827175 None:intergenic
GACAAGAATCCGCTCATTGT+TGG 0.413347 1_3:-58826025 Msa0128990:CDS
ATAGCTTTCTAAAGTTAATT+GGG 0.414342 1_3:-58825913 Msa0128990:three_prime_UTR
AAGCGGGTTTCAGAACTTTA+GGG 0.416206 1_3:+58827302 None:intergenic
GAAGACCAACACGTACCTTT+GGG 0.427653 1_3:+58827335 None:intergenic
GATCCTAAATGGGACGATAA+AGG 0.428418 1_3:-58827241 Msa0128990:CDS
TTGTGGTGTAGATTTGAAAT+CGG 0.469021 1_3:-58827000 Msa0128990:intron
GACACAGAGAGAAGAAGAAT+GGG 0.476563 1_3:-58827388 Msa0128990:exon
GAATTTGAAGAGAATCAGTA+AGG 0.477015 1_3:+58827167 None:intergenic
TGCCATCAAATTTACAATCT+GGG 0.479882 1_3:-58825969 Msa0128990:three_prime_UTR
GTCTTCTTCAAGATCACTGC+CGG 0.487579 1_3:+58827120 None:intergenic
GTAGATTTGAAATCGGCGTT+GGG 0.505626 1_3:-58826993 Msa0128990:intron
AGACACAGAGAGAAGAAGAA+TGG 0.507644 1_3:-58827389 Msa0128990:exon
TCCATGCAAAATTCAAAAGC+AGG 0.510757 1_3:+58825801 None:intergenic
TCAAATTTACAATCTGGGCG+TGG 0.510966 1_3:-58825964 Msa0128990:three_prime_UTR
ATGGCATTGCACTTGTCCAT+AGG 0.520593 1_3:+58825986 None:intergenic
TGAATTGAAGTCAAAGGTTG+AGG 0.526816 1_3:+58826939 None:intergenic
TGTAGATTTGAAATCGGCGT+TGG 0.527237 1_3:-58826994 Msa0128990:intron
ATCAGAAACAACGCCGAAAG+AGG 0.531608 1_3:+58827198 None:intergenic
GCGTGGGTTGTGAGCTGCAA+CGG 0.531681 1_3:-58825947 Msa0128990:three_prime_UTR
GTTGCAACTGAATGCTGCAT+AGG 0.533068 1_3:+58826085 None:intergenic
TTGCAACTGAATGCTGCATA+GGG 0.539277 1_3:+58826086 None:intergenic
CCTTGCAGAAGATCCTAAAT+GGG 0.546570 1_3:-58827251 Msa0128990:CDS
CCATTTAGGATCTTCTGCAA+GGG 0.547386 1_3:+58827252 None:intergenic
TAACTTTAGAAAGCTATCCA+TGG 0.552988 1_3:+58825919 None:intergenic
CCCATTTAGGATCTTCTGCA+AGG 0.554925 1_3:+58827251 None:intergenic
GTTGCCGATGATAACGATCC+CGG 0.559862 1_3:-58827139 Msa0128990:CDS
CAAATTTACAATCTGGGCGT+GGG 0.560830 1_3:-58825963 Msa0128990:three_prime_UTR
TTTGGAGGAATAGTGAAAGC+GGG 0.577554 1_3:+58827286 None:intergenic
TCTTCTTCAAGATCACTGCC+GGG 0.597126 1_3:+58827121 None:intergenic
TCGTTATCATCGGCAACAGA+AGG 0.598477 1_3:+58827145 None:intergenic
GCAACTGCGTAAGCAAGATA+GGG 0.606004 1_3:+58827412 None:intergenic
AGACGCTGAATTGAAGTCAA+AGG 0.608242 1_3:+58826933 None:intergenic
GAGCTGCAACGGTTGATCCA+TGG 0.610724 1_3:-58825936 Msa0128990:three_prime_UTR
AATTTGAAGAGAATCAGTAA+GGG 0.613175 1_3:+58827168 None:intergenic
TTGGGTAAAAGGAGAAGAGA+TGG 0.616129 1_3:-58826975 Msa0128990:CDS
TTTCATTTACCAACAATGAG+CGG 0.625317 1_3:+58826016 None:intergenic
GGCAGTGATCTTGAAGAAGA+CGG 0.629513 1_3:-58827118 Msa0128990:intron
TCTCGCCCAAAGGTACGTGT+TGG 0.632503 1_3:-58827340 Msa0128990:CDS
GTATAAGCAATCTCGCCCAA+AGG 0.632811 1_3:-58827350 Msa0128990:CDS
ACTGCCGGGATCGTTATCAT+CGG 0.664050 1_3:+58827135 None:intergenic
AGCAAAATGTTGTTACAATG+AGG 0.667627 1_3:-58825883 Msa0128990:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTGTTTTGTTTTTATTT+TGG - chr1_3:58827031-58827050 Msa0128990:intron 10.0%
!!! TTATTGTTTTGTTTTTATTT+TGG - chr1_3:58827031-58827050 Msa0128990:intron 10.0%
!! AACTAATTTATTTGCATAAA+CGG + chr1_3:58826625-58826644 None:intergenic 15.0%
!!! CTTTCATCTATTAATTTTTT+TGG + chr1_3:58827441-58827460 None:intergenic 15.0%
!! AACTAATTTATTTGCATAAA+CGG + chr1_3:58826625-58826644 None:intergenic 15.0%
!!! CTTTCATCTATTAATTTTTT+TGG + chr1_3:58827441-58827460 None:intergenic 15.0%
!! GTTGAAATTAGTATTAATCT+TGG - chr1_3:58826110-58826129 Msa0128990:CDS 20.0%
!! GAATGTAAATAATTTGAGTA+TGG - chr1_3:58826144-58826163 Msa0128990:intron 20.0%
!!! TTTATCTTTTTCTATAGAAG+TGG + chr1_3:58826442-58826461 None:intergenic 20.0%
!! ATAAATTTGTGAGATTGTTT+GGG + chr1_3:58826556-58826575 None:intergenic 20.0%
!!! TGTTTTTTCATAAATAACCT+TGG + chr1_3:58826672-58826691 None:intergenic 20.0%
!! GAGTAAAATGTTTATGATTA+GGG + chr1_3:58826812-58826831 None:intergenic 20.0%
!! TGAGTAAAATGTTTATGATT+AGG + chr1_3:58826813-58826832 None:intergenic 20.0%
!!! CATTTTACTCATATACTATA+TGG - chr1_3:58826822-58826841 Msa0128990:intron 20.0%
!! TATCATAATCTTGAAAACAT+CGG - chr1_3:58826879-58826898 Msa0128990:intron 20.0%
!!! ATAGCTTTCTAAAGTTAATT+GGG - chr1_3:58827256-58827275 Msa0128990:CDS 20.0%
!!! TTAATAGATGAAAGTAGTTT+TGG - chr1_3:58827447-58827466 Msa0128990:five_prime_UTR 20.0%
!! GTTGAAATTAGTATTAATCT+TGG - chr1_3:58826110-58826129 Msa0128990:CDS 20.0%
!! GAATGTAAATAATTTGAGTA+TGG - chr1_3:58826144-58826163 Msa0128990:intron 20.0%
!!! TTTATCTTTTTCTATAGAAG+TGG + chr1_3:58826442-58826461 None:intergenic 20.0%
!! ATAAATTTGTGAGATTGTTT+GGG + chr1_3:58826556-58826575 None:intergenic 20.0%
!!! TGTTTTTTCATAAATAACCT+TGG + chr1_3:58826672-58826691 None:intergenic 20.0%
!! GAGTAAAATGTTTATGATTA+GGG + chr1_3:58826812-58826831 None:intergenic 20.0%
!! TGAGTAAAATGTTTATGATT+AGG + chr1_3:58826813-58826832 None:intergenic 20.0%
!!! CATTTTACTCATATACTATA+TGG - chr1_3:58826822-58826841 Msa0128990:intron 20.0%
!! TATCATAATCTTGAAAACAT+CGG - chr1_3:58826879-58826898 Msa0128990:intron 20.0%
!!! ATAGCTTTCTAAAGTTAATT+GGG - chr1_3:58827256-58827275 Msa0128990:CDS 20.0%
!!! TTAATAGATGAAAGTAGTTT+TGG - chr1_3:58827447-58827466 Msa0128990:five_prime_UTR 20.0%
! AATTTGAAGAGAATCAGTAA+GGG + chr1_3:58826004-58826023 None:intergenic 25.0%
! TAACAATTTACGATGAATTG+AGG + chr1_3:58826083-58826102 None:intergenic 25.0%
!! ATTAATCTTGGTAATTCTGA+AGG - chr1_3:58826122-58826141 Msa0128990:intron 25.0%
!!! ATAATTTGAGTATGGTTTTG+TGG - chr1_3:58826152-58826171 Msa0128990:intron 25.0%
! AGTCGTTTAGTTGAAAAATA+TGG - chr1_3:58826266-58826285 Msa0128990:intron 25.0%
!! AAAATATGGAGATGATTTTC+AGG - chr1_3:58826280-58826299 Msa0128990:intron 25.0%
!!! GATAATTTGTTTATCCAAAC+AGG + chr1_3:58826379-58826398 None:intergenic 25.0%
! CATAAATTTGTGAGATTGTT+TGG + chr1_3:58826557-58826576 None:intergenic 25.0%
! TATGAAAAAACAGCTTATGA+AGG - chr1_3:58826680-58826699 Msa0128990:intron 25.0%
! TACTCATATACTATATGGTT+AGG - chr1_3:58826827-58826846 Msa0128990:intron 25.0%
!! TAAATGAAATGTTTTGCCTA+TGG - chr1_3:58827167-58827186 Msa0128990:CDS 25.0%
!!! GATAGCTTTCTAAAGTTAAT+TGG - chr1_3:58827255-58827274 Msa0128990:CDS 25.0%
!! AATTTTGCATGGATTTTTGT+TGG - chr1_3:58827378-58827397 Msa0128990:exon 25.0%
!! ATTTTGCATGGATTTTTGTT+GGG - chr1_3:58827379-58827398 Msa0128990:exon 25.0%
!!! TTTTTTGGCAACAACAATTT+GGG + chr1_3:58827426-58827445 None:intergenic 25.0%
!!! TTTTTTTGGCAACAACAATT+TGG + chr1_3:58827427-58827446 None:intergenic 25.0%
! AATTTGAAGAGAATCAGTAA+GGG + chr1_3:58826004-58826023 None:intergenic 25.0%
! TAACAATTTACGATGAATTG+AGG + chr1_3:58826083-58826102 None:intergenic 25.0%
!! ATTAATCTTGGTAATTCTGA+AGG - chr1_3:58826122-58826141 Msa0128990:intron 25.0%
!!! ATAATTTGAGTATGGTTTTG+TGG - chr1_3:58826152-58826171 Msa0128990:intron 25.0%
! AGTCGTTTAGTTGAAAAATA+TGG - chr1_3:58826266-58826285 Msa0128990:intron 25.0%
!! AAAATATGGAGATGATTTTC+AGG - chr1_3:58826280-58826299 Msa0128990:intron 25.0%
!!! GATAATTTGTTTATCCAAAC+AGG + chr1_3:58826379-58826398 None:intergenic 25.0%
! CATAAATTTGTGAGATTGTT+TGG + chr1_3:58826557-58826576 None:intergenic 25.0%
! TATGAAAAAACAGCTTATGA+AGG - chr1_3:58826680-58826699 Msa0128990:intron 25.0%
! TACTCATATACTATATGGTT+AGG - chr1_3:58826827-58826846 Msa0128990:intron 25.0%
!! TAAATGAAATGTTTTGCCTA+TGG - chr1_3:58827167-58827186 Msa0128990:CDS 25.0%
!!! GATAGCTTTCTAAAGTTAAT+TGG - chr1_3:58827255-58827274 Msa0128990:CDS 25.0%
!! AATTTTGCATGGATTTTTGT+TGG - chr1_3:58827378-58827397 Msa0128990:exon 25.0%
!! ATTTTGCATGGATTTTTGTT+GGG - chr1_3:58827379-58827398 Msa0128990:exon 25.0%
!!! TTTTTTGGCAACAACAATTT+GGG + chr1_3:58827426-58827445 None:intergenic 25.0%
!!! TTTTTTTGGCAACAACAATT+TGG + chr1_3:58827427-58827446 None:intergenic 25.0%
!!! GTTTTTCGAGTATGAGATTA+GGG + chr1_3:58825738-58825757 None:intergenic 30.0%
GAATTTGAAGAGAATCAGTA+AGG + chr1_3:58826005-58826024 None:intergenic 30.0%
! TTGTGGTGTAGATTTGAAAT+CGG - chr1_3:58826169-58826188 Msa0128990:intron 30.0%
ATCTCACAAATTTATGTCAG+TGG - chr1_3:58826561-58826580 Msa0128990:intron 30.0%
TATTATTCATAAGCTTGCCA+AGG - chr1_3:58826652-58826671 Msa0128990:intron 30.0%
! TCTAAAGTAACACAAATGAG+TGG - chr1_3:58826918-58826937 Msa0128990:CDS 30.0%
! CAAATGTTACTCTGTTTTCT+TGG - chr1_3:58826982-58827001 Msa0128990:CDS 30.0%
!! TACTCTGTTTTCTTGGTTTT+AGG - chr1_3:58826989-58827008 Msa0128990:CDS 30.0%
!!! GTTTTAGGACTTCATTTATG+TGG - chr1_3:58827004-58827023 Msa0128990:intron 30.0%
!! ATTTTGGCAGAAAATGATGA+TGG - chr1_3:58827047-58827066 Msa0128990:intron 30.0%
TTTCATTTACCAACAATGAG+CGG + chr1_3:58827156-58827175 None:intergenic 30.0%
ATGCCATCAAATTTACAATC+TGG - chr1_3:58827199-58827218 Msa0128990:CDS 30.0%
TGCCATCAAATTTACAATCT+GGG - chr1_3:58827200-58827219 Msa0128990:CDS 30.0%
! TAACTTTAGAAAGCTATCCA+TGG + chr1_3:58827253-58827272 None:intergenic 30.0%
AGCAAAATGTTGTTACAATG+AGG - chr1_3:58827286-58827305 Msa0128990:CDS 30.0%
!!! TAGATGAAAGTAGTTTTGGA+TGG - chr1_3:58827451-58827470 Msa0128990:five_prime_UTR 30.0%
! TGGAATGTGATAAGTTGTTT+TGG - chr1_3:58827471-58827490 Msa0128990:five_prime_UTR 30.0%
!! GGAATGTGATAAGTTGTTTT+GGG - chr1_3:58827472-58827491 Msa0128990:five_prime_UTR 30.0%
!!! GTTTTTCGAGTATGAGATTA+GGG + chr1_3:58825738-58825757 None:intergenic 30.0%
GAATTTGAAGAGAATCAGTA+AGG + chr1_3:58826005-58826024 None:intergenic 30.0%
! TTGTGGTGTAGATTTGAAAT+CGG - chr1_3:58826169-58826188 Msa0128990:intron 30.0%
ATCTCACAAATTTATGTCAG+TGG - chr1_3:58826561-58826580 Msa0128990:intron 30.0%
TATTATTCATAAGCTTGCCA+AGG - chr1_3:58826652-58826671 Msa0128990:intron 30.0%
! TCTAAAGTAACACAAATGAG+TGG - chr1_3:58826918-58826937 Msa0128990:CDS 30.0%
! CAAATGTTACTCTGTTTTCT+TGG - chr1_3:58826982-58827001 Msa0128990:CDS 30.0%
!! TACTCTGTTTTCTTGGTTTT+AGG - chr1_3:58826989-58827008 Msa0128990:CDS 30.0%
!!! GTTTTAGGACTTCATTTATG+TGG - chr1_3:58827004-58827023 Msa0128990:intron 30.0%
!! ATTTTGGCAGAAAATGATGA+TGG - chr1_3:58827047-58827066 Msa0128990:intron 30.0%
TTTCATTTACCAACAATGAG+CGG + chr1_3:58827156-58827175 None:intergenic 30.0%
ATGCCATCAAATTTACAATC+TGG - chr1_3:58827199-58827218 Msa0128990:CDS 30.0%
TGCCATCAAATTTACAATCT+GGG - chr1_3:58827200-58827219 Msa0128990:CDS 30.0%
! TAACTTTAGAAAGCTATCCA+TGG + chr1_3:58827253-58827272 None:intergenic 30.0%
AGCAAAATGTTGTTACAATG+AGG - chr1_3:58827286-58827305 Msa0128990:CDS 30.0%
!!! TAGATGAAAGTAGTTTTGGA+TGG - chr1_3:58827451-58827470 Msa0128990:five_prime_UTR 30.0%
! TGGAATGTGATAAGTTGTTT+TGG - chr1_3:58827471-58827490 Msa0128990:five_prime_UTR 30.0%
GAGTATGAGATTAGGGTTTA+GGG + chr1_3:58825731-58825750 None:intergenic 35.0%
!!! GGTTTTTCGAGTATGAGATT+AGG + chr1_3:58825739-58825758 None:intergenic 35.0%
!! AGAACTTTAGGGTTCTTCTT+TGG + chr1_3:58825859-58825878 None:intergenic 35.0%
!! TTTTGGAGGAATAGTGAAAG+CGG + chr1_3:58825887-58825906 None:intergenic 35.0%
ACCCAAAACTATTCCTCTTT+CGG - chr1_3:58825958-58825977 Msa0128990:three_prime_UTR 35.0%
TGAATTGAAGTCAAAGGTTG+AGG + chr1_3:58826233-58826252 None:intergenic 35.0%
!!! CGTTTTTTTCACTGTTGCTT+TGG - chr1_3:58826305-58826324 Msa0128990:intron 35.0%
! AGCTTTGATAGTTCACTTCT+AGG - chr1_3:58826352-58826371 Msa0128990:intron 35.0%
ACTGTCCCAATAAACCTTTA+GGG - chr1_3:58826599-58826618 Msa0128990:intron 35.0%
!! TGAAGGTGTACAATTTTCGT+CGG - chr1_3:58826697-58826716 Msa0128990:intron 35.0%
! ATGATTAGGGCTCTTTTGAT+TGG + chr1_3:58826799-58826818 None:intergenic 35.0%
!! TCCTGCTTTTGAATTTTGCA+TGG - chr1_3:58827367-58827386 Msa0128990:CDS 35.0%
TCCATGCAAAATTCAAAAGC+AGG + chr1_3:58827371-58827390 None:intergenic 35.0%
TTGGGAAAGATGTAGTATTC+TGG - chr1_3:58827397-58827416 Msa0128990:five_prime_UTR 35.0%
GAGTATGAGATTAGGGTTTA+GGG + chr1_3:58825731-58825750 None:intergenic 35.0%
!!! GGTTTTTCGAGTATGAGATT+AGG + chr1_3:58825739-58825758 None:intergenic 35.0%
!! AGAACTTTAGGGTTCTTCTT+TGG + chr1_3:58825859-58825878 None:intergenic 35.0%
!! TTTTGGAGGAATAGTGAAAG+CGG + chr1_3:58825887-58825906 None:intergenic 35.0%
ACCCAAAACTATTCCTCTTT+CGG - chr1_3:58825958-58825977 Msa0128990:three_prime_UTR 35.0%
TGAATTGAAGTCAAAGGTTG+AGG + chr1_3:58826233-58826252 None:intergenic 35.0%
!!! CGTTTTTTTCACTGTTGCTT+TGG - chr1_3:58826305-58826324 Msa0128990:intron 35.0%
! AGCTTTGATAGTTCACTTCT+AGG - chr1_3:58826352-58826371 Msa0128990:intron 35.0%
ACTGTCCCAATAAACCTTTA+GGG - chr1_3:58826599-58826618 Msa0128990:intron 35.0%
!! TGAAGGTGTACAATTTTCGT+CGG - chr1_3:58826697-58826716 Msa0128990:intron 35.0%
! ATGATTAGGGCTCTTTTGAT+TGG + chr1_3:58826799-58826818 None:intergenic 35.0%
!! TCCTGCTTTTGAATTTTGCA+TGG - chr1_3:58827367-58827386 Msa0128990:CDS 35.0%
TCCATGCAAAATTCAAAAGC+AGG + chr1_3:58827371-58827390 None:intergenic 35.0%
TTGGGAAAGATGTAGTATTC+TGG - chr1_3:58827397-58827416 Msa0128990:five_prime_UTR 35.0%
CGAGTATGAGATTAGGGTTT+AGG + chr1_3:58825732-58825751 None:intergenic 40.0%
AGACACAGAGAGAAGAAGAA+TGG - chr1_3:58825780-58825799 Msa0128990:three_prime_UTR 40.0%
GACACAGAGAGAAGAAGAAT+GGG - chr1_3:58825781-58825800 Msa0128990:three_prime_UTR 40.0%
! AAGCGGGTTTCAGAACTTTA+GGG + chr1_3:58825870-58825889 None:intergenic 40.0%
! AAAGCGGGTTTCAGAACTTT+AGG + chr1_3:58825871-58825890 None:intergenic 40.0%
TTTGGAGGAATAGTGAAAGC+GGG + chr1_3:58825886-58825905 None:intergenic 40.0%
!! AGGGATTGTAACAGTTTTGG+AGG + chr1_3:58825901-58825920 None:intergenic 40.0%
! GCAAGGGATTGTAACAGTTT+TGG + chr1_3:58825904-58825923 None:intergenic 40.0%
! CCATTTAGGATCTTCTGCAA+GGG + chr1_3:58825920-58825939 None:intergenic 40.0%
CCTTGCAGAAGATCCTAAAT+GGG - chr1_3:58825918-58825937 Msa0128990:three_prime_UTR 40.0%
GATCCTAAATGGGACGATAA+AGG - chr1_3:58825928-58825947 Msa0128990:three_prime_UTR 40.0%
! GCCGAAAGAGGAATAGTTTT+GGG + chr1_3:58825962-58825981 None:intergenic 40.0%
AGAGAATCAGTAAGGGAGTT+AGG + chr1_3:58825997-58826016 None:intergenic 40.0%
! TGTAGATTTGAAATCGGCGT+TGG - chr1_3:58826175-58826194 Msa0128990:intron 40.0%
! GTAGATTTGAAATCGGCGTT+GGG - chr1_3:58826176-58826195 Msa0128990:intron 40.0%
TTGGGTAAAAGGAGAAGAGA+TGG - chr1_3:58826194-58826213 Msa0128990:intron 40.0%
AGACGCTGAATTGAAGTCAA+AGG + chr1_3:58826239-58826258 None:intergenic 40.0%
GACTGTCCCAATAAACCTTT+AGG - chr1_3:58826598-58826617 Msa0128990:intron 40.0%
AACGGCCCTAAAGGTTTATT+GGG + chr1_3:58826607-58826626 None:intergenic 40.0%
AAACGGCCCTAAAGGTTTAT+TGG + chr1_3:58826608-58826627 None:intergenic 40.0%
ATTTGCATAAACGGCCCTAA+AGG + chr1_3:58826616-58826635 None:intergenic 40.0%
!! GAAGGTGTACAATTTTCGTC+GGG - chr1_3:58826698-58826717 Msa0128990:intron 40.0%
! AAGGTGTACAATTTTCGTCG+GGG - chr1_3:58826699-58826718 Msa0128990:intron 40.0%
!! TGAGTGGTTGTGATTTGTTG+AGG - chr1_3:58826934-58826953 Msa0128990:CDS 40.0%
TTGCAACTGAATGCTGCATA+GGG + chr1_3:58827086-58827105 None:intergenic 40.0%
CGCCCAGATTGTAAATTTGA+TGG + chr1_3:58827205-58827224 None:intergenic 40.0%
TCAAATTTACAATCTGGGCG+TGG - chr1_3:58827205-58827224 Msa0128990:CDS 40.0%
CAAATTTACAATCTGGGCGT+GGG - chr1_3:58827206-58827225 Msa0128990:CDS 40.0%
CGAGTATGAGATTAGGGTTT+AGG + chr1_3:58825732-58825751 None:intergenic 40.0%
AGACACAGAGAGAAGAAGAA+TGG - chr1_3:58825780-58825799 Msa0128990:three_prime_UTR 40.0%
GACACAGAGAGAAGAAGAAT+GGG - chr1_3:58825781-58825800 Msa0128990:three_prime_UTR 40.0%
! AAGCGGGTTTCAGAACTTTA+GGG + chr1_3:58825870-58825889 None:intergenic 40.0%
! AAAGCGGGTTTCAGAACTTT+AGG + chr1_3:58825871-58825890 None:intergenic 40.0%
TTTGGAGGAATAGTGAAAGC+GGG + chr1_3:58825886-58825905 None:intergenic 40.0%
!! AGGGATTGTAACAGTTTTGG+AGG + chr1_3:58825901-58825920 None:intergenic 40.0%
! GCAAGGGATTGTAACAGTTT+TGG + chr1_3:58825904-58825923 None:intergenic 40.0%
! CCATTTAGGATCTTCTGCAA+GGG + chr1_3:58825920-58825939 None:intergenic 40.0%
CCTTGCAGAAGATCCTAAAT+GGG - chr1_3:58825918-58825937 Msa0128990:three_prime_UTR 40.0%
GATCCTAAATGGGACGATAA+AGG - chr1_3:58825928-58825947 Msa0128990:three_prime_UTR 40.0%
! GCCGAAAGAGGAATAGTTTT+GGG + chr1_3:58825962-58825981 None:intergenic 40.0%
AGAGAATCAGTAAGGGAGTT+AGG + chr1_3:58825997-58826016 None:intergenic 40.0%
! TGTAGATTTGAAATCGGCGT+TGG - chr1_3:58826175-58826194 Msa0128990:intron 40.0%
! GTAGATTTGAAATCGGCGTT+GGG - chr1_3:58826176-58826195 Msa0128990:intron 40.0%
TTGGGTAAAAGGAGAAGAGA+TGG - chr1_3:58826194-58826213 Msa0128990:intron 40.0%
AGACGCTGAATTGAAGTCAA+AGG + chr1_3:58826239-58826258 None:intergenic 40.0%
GACTGTCCCAATAAACCTTT+AGG - chr1_3:58826598-58826617 Msa0128990:intron 40.0%
AACGGCCCTAAAGGTTTATT+GGG + chr1_3:58826607-58826626 None:intergenic 40.0%
AAACGGCCCTAAAGGTTTAT+TGG + chr1_3:58826608-58826627 None:intergenic 40.0%
ATTTGCATAAACGGCCCTAA+AGG + chr1_3:58826616-58826635 None:intergenic 40.0%
!! GAAGGTGTACAATTTTCGTC+GGG - chr1_3:58826698-58826717 Msa0128990:intron 40.0%
! AAGGTGTACAATTTTCGTCG+GGG - chr1_3:58826699-58826718 Msa0128990:intron 40.0%
!! TGAGTGGTTGTGATTTGTTG+AGG - chr1_3:58826934-58826953 Msa0128990:CDS 40.0%
TTGCAACTGAATGCTGCATA+GGG + chr1_3:58827086-58827105 None:intergenic 40.0%
CGCCCAGATTGTAAATTTGA+TGG + chr1_3:58827205-58827224 None:intergenic 40.0%
TCAAATTTACAATCTGGGCG+TGG - chr1_3:58827205-58827224 Msa0128990:CDS 40.0%
CAAATTTACAATCTGGGCGT+GGG - chr1_3:58827206-58827225 Msa0128990:CDS 40.0%
GCAACTGCGTAAGCAAGATA+GGG + chr1_3:58825760-58825779 None:intergenic 45.0%
TGCAACTGCGTAAGCAAGAT+AGG + chr1_3:58825761-58825780 None:intergenic 45.0%
GTATAAGCAATCTCGCCCAA+AGG - chr1_3:58825819-58825838 Msa0128990:three_prime_UTR 45.0%
GAAGACCAACACGTACCTTT+GGG + chr1_3:58825837-58825856 None:intergenic 45.0%
CCCTTGCAGAAGATCCTAAA+TGG - chr1_3:58825917-58825936 Msa0128990:three_prime_UTR 45.0%
! CCCATTTAGGATCTTCTGCA+AGG + chr1_3:58825921-58825940 None:intergenic 45.0%
GAGCCTTTATCGTCCCATTT+AGG + chr1_3:58825934-58825953 None:intergenic 45.0%
! CGCCGAAAGAGGAATAGTTT+TGG + chr1_3:58825963-58825982 None:intergenic 45.0%
ATCAGAAACAACGCCGAAAG+AGG + chr1_3:58825974-58825993 None:intergenic 45.0%
TCGTTATCATCGGCAACAGA+AGG + chr1_3:58826027-58826046 None:intergenic 45.0%
TCTTCTTCAAGATCACTGCC+GGG + chr1_3:58826051-58826070 None:intergenic 45.0%
GTCTTCTTCAAGATCACTGC+CGG + chr1_3:58826052-58826071 None:intergenic 45.0%
GGCAGTGATCTTGAAGAAGA+CGG - chr1_3:58826051-58826070 Msa0128990:CDS 45.0%
! TGAAATCGGCGTTGGGTAAA+AGG - chr1_3:58826183-58826202 Msa0128990:intron 45.0%
GTTCACTTCTAGGTCCTGTT+TGG - chr1_3:58826362-58826381 Msa0128990:intron 45.0%
GTTGCAACTGAATGCTGCAT+AGG + chr1_3:58827087-58827106 None:intergenic 45.0%
GACAAGAATCCGCTCATTGT+TGG - chr1_3:58827144-58827163 Msa0128990:CDS 45.0%
ATGGCATTGCACTTGTCCAT+AGG + chr1_3:58827186-58827205 None:intergenic 45.0%
GCAACTGCGTAAGCAAGATA+GGG + chr1_3:58825760-58825779 None:intergenic 45.0%
TGCAACTGCGTAAGCAAGAT+AGG + chr1_3:58825761-58825780 None:intergenic 45.0%
GTATAAGCAATCTCGCCCAA+AGG - chr1_3:58825819-58825838 Msa0128990:three_prime_UTR 45.0%
GAAGACCAACACGTACCTTT+GGG + chr1_3:58825837-58825856 None:intergenic 45.0%
CCCTTGCAGAAGATCCTAAA+TGG - chr1_3:58825917-58825936 Msa0128990:three_prime_UTR 45.0%
! CCCATTTAGGATCTTCTGCA+AGG + chr1_3:58825921-58825940 None:intergenic 45.0%
GAGCCTTTATCGTCCCATTT+AGG + chr1_3:58825934-58825953 None:intergenic 45.0%
! CGCCGAAAGAGGAATAGTTT+TGG + chr1_3:58825963-58825982 None:intergenic 45.0%
ATCAGAAACAACGCCGAAAG+AGG + chr1_3:58825974-58825993 None:intergenic 45.0%
TCGTTATCATCGGCAACAGA+AGG + chr1_3:58826027-58826046 None:intergenic 45.0%
TCTTCTTCAAGATCACTGCC+GGG + chr1_3:58826051-58826070 None:intergenic 45.0%
GTCTTCTTCAAGATCACTGC+CGG + chr1_3:58826052-58826071 None:intergenic 45.0%
GGCAGTGATCTTGAAGAAGA+CGG - chr1_3:58826051-58826070 Msa0128990:CDS 45.0%
! TGAAATCGGCGTTGGGTAAA+AGG - chr1_3:58826183-58826202 Msa0128990:intron 45.0%
GTTCACTTCTAGGTCCTGTT+TGG - chr1_3:58826362-58826381 Msa0128990:intron 45.0%
GTTGCAACTGAATGCTGCAT+AGG + chr1_3:58827087-58827106 None:intergenic 45.0%
GACAAGAATCCGCTCATTGT+TGG - chr1_3:58827144-58827163 Msa0128990:CDS 45.0%
ATGGCATTGCACTTGTCCAT+AGG + chr1_3:58827186-58827205 None:intergenic 45.0%
GGAAGACCAACACGTACCTT+TGG + chr1_3:58825838-58825857 None:intergenic 50.0%
! GTTGCCGATGATAACGATCC+CGG - chr1_3:58826030-58826049 Msa0128990:CDS 50.0%
ACTGCCGGGATCGTTATCAT+CGG + chr1_3:58826037-58826056 None:intergenic 50.0%
GGAAGACCAACACGTACCTT+TGG + chr1_3:58825838-58825857 None:intergenic 50.0%
! GTTGCCGATGATAACGATCC+CGG - chr1_3:58826030-58826049 Msa0128990:CDS 50.0%
ACTGCCGGGATCGTTATCAT+CGG + chr1_3:58826037-58826056 None:intergenic 50.0%
! TCTCGCCCAAAGGTACGTGT+TGG - chr1_3:58825829-58825848 Msa0128990:three_prime_UTR 55.0%
GAGCTGCAACGGTTGATCCA+TGG - chr1_3:58827233-58827252 Msa0128990:CDS 55.0%
! TCTCGCCCAAAGGTACGTGT+TGG - chr1_3:58825829-58825848 Msa0128990:three_prime_UTR 55.0%
GAGCTGCAACGGTTGATCCA+TGG - chr1_3:58827233-58827252 Msa0128990:CDS 55.0%
! GCGTGGGTTGTGAGCTGCAA+CGG - chr1_3:58827222-58827241 Msa0128990:CDS 60.0%
! GCGTGGGTTGTGAGCTGCAA+CGG - chr1_3:58827222-58827241 Msa0128990:CDS 60.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 58825697 58827494 58825697 ID=Msa0128990;Name=Msa0128990
chr1_3 mRNA 58825697 58827494 58825697 ID=Msa0128990-mRNA-1;Parent=Msa0128990;Name=Msa0128990-mRNA-1;_AED=0.03;_eAED=0.03;_QI=102|1|1|1|1|1|3|321|170
chr1_3 exon 58825697 58826134 58825697 ID=Msa0128990-mRNA-1:exon:19037;Parent=Msa0128990-mRNA-1
chr1_3 exon 58826890 58827011 58826890 ID=Msa0128990-mRNA-1:exon:19036;Parent=Msa0128990-mRNA-1
chr1_3 exon 58827119 58827494 58827119 ID=Msa0128990-mRNA-1:exon:19035;Parent=Msa0128990-mRNA-1
chr1_3 five_prime_UTR 58827393 58827494 58827393 ID=Msa0128990-mRNA-1:five_prime_utr;Parent=Msa0128990-mRNA-1
chr1_3 CDS 58827119 58827392 58827119 ID=Msa0128990-mRNA-1:cds;Parent=Msa0128990-mRNA-1
chr1_3 CDS 58826890 58827011 58826890 ID=Msa0128990-mRNA-1:cds;Parent=Msa0128990-mRNA-1
chr1_3 CDS 58826018 58826134 58826018 ID=Msa0128990-mRNA-1:cds;Parent=Msa0128990-mRNA-1
chr1_3 three_prime_UTR 58825697 58826017 58825697 ID=Msa0128990-mRNA-1:three_prime_utr;Parent=Msa0128990-mRNA-1
Gene Sequence

>Msa0128990

ATGGGAAGATCACGAAGAAAGTATAAGCAATCTCGCCCAAAGGTACGTGTTGGTCTTCCAAAGAAGAACCCTAAAGTTCTGAAACCCGCTTTCACTATTCCTCCAAAACTGTTACAATCCCTTGCAGAAGATCCTAAATGGGACGATAAAGGCTCTGTCACCCAAAACTATTCCTCTTTCGGCGTTGTTTCTGATCCTAACTCCCTTACTGATTCTCTTCAAATTCCTTCTGTTGCCGATGATAACGATCCCGGCAGTGATCTTGAAGAAGACGATTTGAAATCGGCGTTGGGTAAAAGGAGAAGAGATGGTAAAAGCTCACTTCCTCAACCTTTGACTTCAATTCAGCGTCTTCATATTAGTCGTTTAGTTGAAAAATATGGAGATGATTTTCAGAAAATGATGATGGATATAAAGCTAAACCCTATGCAGCATTCAGTTGCAACTTTAGAGAAATTGTGCTTCAGCTATTACTTATATAAAGACAAGAATCCGCTCATTGTTGGTAAATGA
Protein sequence

>Msa0128990

MGRSRRKYKQSRPKVRVGLPKKNPKVLKPAFTIPPKLLQSLAEDPKWDDKGSVTQNYSSFGVVSDPNSLTDSLQIPSVADDNDPGSDLEEDDLKSALGKRRRDGKSSLPQPLTSIQRLHISRLVEKYGDDFQKMMMDIKLNPMQHSVATLEKLCFSYYLYKDKNPLIVGK*