Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0130640 | AES62102.2 | 96.721 | 61 | 2 | 0 | 1 | 61 | 1 | 61 | 2.32e-29 | 108 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0130640 | G7I4Y9 | 96.721 | 61 | 2 | 0 | 1 | 61 | 1 | 61 | 1.11e-29 | 108 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0084570 | Msa0130640 | 0.926849 | 2.662639e-91 | -8.615850e-47 |
| Msa0130640 | Msa1081600 | 0.825031 | 5.850836e-54 | -8.615850e-47 |
| Msa0130640 | Msa1166370 | 0.822762 | 1.991804e-53 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0130640 | MtrunA17_Chr1g0198951 | 96.721 | 61 | 2 | 0 | 1 | 61 | 1 | 61 | 2.14e-33 | 108 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 12 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTAGGAGTAGCTTCTTTCT+TGG | 0.263984 | 1_3:-61036822 | None:intergenic |
| AGCTTTCTTTGAAGCTGATT+TGG | 0.275824 | 1_3:-61036867 | None:intergenic |
| TGACCCTTATCACTAGCTTT+AGG | 0.299140 | 1_3:-61036839 | None:intergenic |
| TCAAGATCTTAATAAGAAAA+AGG | 0.310643 | 1_3:+61036901 | Msa0130640:CDS |
| TACTCCTAAAGCTAGTGATA+AGG | 0.421532 | 1_3:+61036835 | Msa0130640:CDS |
| ACTCCTAAAGCTAGTGATAA+GGG | 0.443378 | 1_3:+61036836 | Msa0130640:CDS |
| AACAGGTTTATCAGCCTTCA+AGG | 0.447580 | 1_3:-61036777 | None:intergenic |
| GTGCAAAATGGCATCCTTGA+AGG | 0.478600 | 1_3:+61036763 | Msa0130640:exon |
| CTTGGTTGTCTACAGGGAAA+AGG | 0.487214 | 1_3:+61037047 | Msa0130640:intron |
| TATTGTATTCAATTCAATGT+TGG | 0.499529 | 1_3:-61037151 | None:intergenic |
| TATTCAATTCAATGTTGGTG+AGG | 0.563987 | 1_3:-61037146 | None:intergenic |
| TTGAAGGCTGATAAACCTGT+TGG | 0.591373 | 1_3:+61036779 | Msa0130640:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | CTTTTTTTTTTAGTAAAGTA+AGG | - | chr1_3:61037105-61037124 | None:intergenic | 15.0% |
| !!! | TAAAGATATTTTGTGCAAAA+TGG | + | chr1_3:61036751-61036770 | Msa0130640:exon | 20.0% |
| !!! | TTTTTCTTATTAAGATCTTG+AGG | - | chr1_3:61036902-61036921 | None:intergenic | 20.0% |
| !! | TCAAGATCTTAATAAGAAAA+AGG | + | chr1_3:61036901-61036920 | Msa0130640:CDS | 20.0% |
| !!! | TTTTAATCTTGCTTATATGA+TGG | + | chr1_3:61036954-61036973 | Msa0130640:intron | 20.0% |
| !! | TAAGCACATATATAGTAATA+GGG | - | chr1_3:61037002-61037021 | None:intergenic | 20.0% |
| !!! | TCAACTTTTTTTTTTATCCT+TGG | + | chr1_3:61037029-61037048 | Msa0130640:intron | 20.0% |
| !! | TATTGTATTCAATTCAATGT+TGG | - | chr1_3:61037154-61037173 | None:intergenic | 20.0% |
| ! | GTAAGCACATATATAGTAAT+AGG | - | chr1_3:61037003-61037022 | None:intergenic | 25.0% |
| TAATCTTGCTTATATGATGG+TGG | + | chr1_3:61036957-61036976 | Msa0130640:intron | 30.0% | |
| TATTCAATTCAATGTTGGTG+AGG | - | chr1_3:61037149-61037168 | None:intergenic | 30.0% | |
| TTTAGGAGTAGCTTCTTTCT+TGG | - | chr1_3:61036825-61036844 | None:intergenic | 35.0% | |
| TACTCCTAAAGCTAGTGATA+AGG | + | chr1_3:61036835-61036854 | Msa0130640:CDS | 35.0% | |
| ACTCCTAAAGCTAGTGATAA+GGG | + | chr1_3:61036836-61036855 | Msa0130640:CDS | 35.0% | |
| !! | AGCTTTCTTTGAAGCTGATT+TGG | - | chr1_3:61036870-61036889 | None:intergenic | 35.0% |
| ! | TTTTATCCTTGGTTGTCTAC+AGG | + | chr1_3:61037040-61037059 | Msa0130640:intron | 35.0% |
| TTTATCCTTGGTTGTCTACA+GGG | + | chr1_3:61037041-61037060 | Msa0130640:intron | 35.0% | |
| AACAGGTTTATCAGCCTTCA+AGG | - | chr1_3:61036780-61036799 | None:intergenic | 40.0% | |
| TTGAAGGCTGATAAACCTGT+TGG | + | chr1_3:61036779-61036798 | Msa0130640:CDS | 40.0% | |
| !! | TGACCCTTATCACTAGCTTT+AGG | - | chr1_3:61036842-61036861 | None:intergenic | 40.0% |
| GTGCAAAATGGCATCCTTGA+AGG | + | chr1_3:61036763-61036782 | Msa0130640:exon | 45.0% | |
| ! | CTGTTGGCACCCAACTTTTT+GGG | + | chr1_3:61036795-61036814 | Msa0130640:CDS | 45.0% |
| ! | CTTTTCCCTGTAGACAACCA+AGG | - | chr1_3:61037049-61037068 | None:intergenic | 45.0% |
| CTTGGTTGTCTACAGGGAAA+AGG | + | chr1_3:61037047-61037066 | Msa0130640:intron | 45.0% | |
| CCAAAAAGTTGGGTGCCAAC+AGG | - | chr1_3:61036797-61036816 | None:intergenic | 50.0% | |
| ! | CCTGTTGGCACCCAACTTTT+TGG | + | chr1_3:61036794-61036813 | Msa0130640:CDS | 50.0% |
| CTTGGCCTGCCCAAAAAGTT+GGG | - | chr1_3:61036807-61036826 | None:intergenic | 50.0% | |
| TCTTGGCCTGCCCAAAAAGT+TGG | - | chr1_3:61036808-61036827 | None:intergenic | 50.0% | |
| !! | TGGCACCCAACTTTTTGGGC+AGG | + | chr1_3:61036799-61036818 | Msa0130640:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_3 | gene | 61036734 | 61037185 | 61036734 | ID=Msa0130640;Name=Msa0130640 |
| chr1_3 | mRNA | 61036734 | 61037185 | 61036734 | ID=Msa0130640-mRNA-1;Parent=Msa0130640;Name=Msa0130640-mRNA-1;_AED=0.00;_eAED=0.00;_QI=36|1|1|1|1|1|2|91|61 |
| chr1_3 | exon | 61036734 | 61036922 | 61036734 | ID=Msa0130640-mRNA-1:exon:20131;Parent=Msa0130640-mRNA-1 |
| chr1_3 | exon | 61037062 | 61037185 | 61037062 | ID=Msa0130640-mRNA-1:exon:20132;Parent=Msa0130640-mRNA-1 |
| chr1_3 | five_prime_UTR | 61036734 | 61036769 | 61036734 | ID=Msa0130640-mRNA-1:five_prime_utr;Parent=Msa0130640-mRNA-1 |
| chr1_3 | CDS | 61036770 | 61036922 | 61036770 | ID=Msa0130640-mRNA-1:cds;Parent=Msa0130640-mRNA-1 |
| chr1_3 | CDS | 61037062 | 61037094 | 61037062 | ID=Msa0130640-mRNA-1:cds;Parent=Msa0130640-mRNA-1 |
| chr1_3 | three_prime_UTR | 61037095 | 61037185 | 61037095 | ID=Msa0130640-mRNA-1:three_prime_utr;Parent=Msa0130640-mRNA-1 |
| Gene Sequence |
| Protein sequence |
