AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0133330

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0133330 MtrunA17_Chr4g0033801 93.750 160 10 0 22 181 6 165 2.36e-98 297
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0133330 AT5G40520.2 39.041 146 79 4 17 160 7 144 1.44e-23 97.1

Find 117 sgRNAs with CRISPR-Local

Find 208 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTAAGGAGTATGAAATTT+TGG 0.141746 1_3:-64599038 None:intergenic
ACCAAATACCAAACTTAAAA+AGG 0.178603 1_3:+64596757 Msa0133330:CDS
ATAATAGGACTCAGGCTTTC+AGG 0.199003 1_3:+64598898 Msa0133330:three_prime_UTR
CTTCTTCAATATGCTTCTTC+TGG 0.209658 1_3:-64597622 None:intergenic
TTCTCACTAAAACTGATAAT+TGG 0.229390 1_3:+64597287 Msa0133330:three_prime_UTR
GAACTGTGATACAATAAATT+TGG 0.238520 1_3:+64597868 Msa0133330:three_prime_UTR
TCTTGGCATTGGTTATTATA+TGG 0.244429 1_3:-64597911 None:intergenic
TATTCACTTGTCTGATTAGA+TGG 0.250819 1_3:-64597181 None:intergenic
TATATTGTCTGTTGAGAAAA+AGG 0.250997 1_3:+64597388 Msa0133330:three_prime_UTR
ACTGCTCTGTGATTTCTTCT+TGG 0.263092 1_3:-64597928 None:intergenic
AAATGATGCAGGTGGTTATT+AGG 0.268573 1_3:+64596358 Msa0133330:intron
GCAATGCGTAGAAGACTTAA+AGG 0.269672 1_3:+64597104 Msa0133330:three_prime_UTR
ACAAAGCTTCAATTAATTTC+AGG 0.280363 1_3:+64599085 Msa0133330:three_prime_UTR
TCTCTTTGAGTGCAGTGATA+TGG 0.305905 1_3:+64597520 Msa0133330:three_prime_UTR
CAACTTCTTCCCCTCCTTTG+TGG 0.306669 1_3:-64596392 None:intergenic
CTAAAACTGATAATTGGTTT+TGG 0.323266 1_3:+64597293 Msa0133330:three_prime_UTR
TAAGTTTGATTCGTTTATCT+TGG 0.323820 1_3:-64596260 None:intergenic
ATTGACATGGCTTTGGCTAA+TGG 0.329725 1_3:-64597257 None:intergenic
TTGAAAGAAATTTGCAAAAC+CGG 0.334175 1_3:+64596412 Msa0133330:CDS
ATTGATGGACTTATGATAAT+AGG 0.335665 1_3:+64598883 Msa0133330:three_prime_UTR
CAAAAGTTGGATTCATCTCT+TGG 0.336309 1_3:-64598067 None:intergenic
TCCGCTGTACTTGGTGGGTA+TGG 0.339166 1_3:-64598104 None:intergenic
CTCTGCAGGGATTTCACTTC+AGG 0.340422 1_3:-64597350 None:intergenic
TACGCATTGCAAGCAAGGTT+TGG 0.340862 1_3:-64597091 None:intergenic
CTGTGATTTCTTCTTGGCAT+TGG 0.350204 1_3:-64597922 None:intergenic
TTGTATCCCTCAGGATGATA+TGG 0.350338 1_3:+64596728 Msa0133330:CDS
GGGCTTTACAGATGCGTATA+TGG 0.364505 1_3:+64597724 Msa0133330:three_prime_UTR
TTATTTACTACTGTAGCCTT+TGG 0.367086 1_3:-64597458 None:intergenic
ACGCATTGCAAGCAAGGTTT+GGG 0.377969 1_3:-64597090 None:intergenic
AAGCAAAGTGAAGACATAAA+AGG 0.380673 1_3:+64598274 Msa0133330:three_prime_UTR
GTTTGCGAGTTTCCAGTTGT+TGG 0.386231 1_3:-64596941 None:intergenic
GTTCTTGTGTCCGCTGTACT+TGG 0.390736 1_3:-64598113 None:intergenic
ATCATTGGCGCTAAAATATT+CGG 0.394849 1_3:-64599113 None:intergenic
TACCTTCTTGCTATGATAGT+TGG 0.398012 1_3:+64596822 Msa0133330:CDS
CATCTTGGGCAGCAAGGTAT+TGG 0.398219 1_3:-64598135 None:intergenic
GTCTGCTATTGACATGGCTT+TGG 0.401689 1_3:-64597264 None:intergenic
ATCTTGTCTGAGTAATGATT+CGG 0.404131 1_3:-64596531 None:intergenic
AATTATTCAAGACTTGTTGC+CGG 0.419014 1_3:+64597685 Msa0133330:three_prime_UTR
AACAGAAGAGAATTGCTCTA+AGG 0.420044 1_3:-64599053 None:intergenic
CATTTCAAAATGCTCTTCGC+TGG 0.420868 1_3:-64598926 None:intergenic
AGAATCTGCAATAGAGACTT+TGG 0.423522 1_3:-64597551 None:intergenic
TAAAATTGTGACTCAGATCT+AGG 0.438338 1_3:-64597020 None:intergenic
TCCTCAATTACTCGAACAAT+AGG 0.442380 1_3:-64598522 None:intergenic
TCTTACTCAAGTCTAAGTTT+AGG 0.443346 1_3:-64598231 None:intergenic
TGTCTTATCACAAGCATCTT+GGG 0.446020 1_3:-64598149 None:intergenic
CAAGGTTGCAGCTGAGGCAT+CGG 0.452977 1_3:-64597137 None:intergenic
CCGCTGTACTTGGTGGGTAT+GGG 0.453476 1_3:-64598103 None:intergenic
GGAAAGACTGAAGATATTGA+TGG 0.454052 1_3:+64598868 Msa0133330:three_prime_UTR
CTTTCATAATATCACTCTGC+AGG 0.457564 1_3:-64597364 None:intergenic
ATGCTTGCATATAATCTCAT+AGG 0.457983 1_3:+64598175 Msa0133330:three_prime_UTR
CCCTGGTCAAATTCATAGTC+CGG 0.460102 1_3:-64597704 None:intergenic
ATCACTATTCAAGCATTCAC+TGG 0.466056 1_3:-64597837 None:intergenic
GAATGTAACAAATACCTCTC+TGG 0.466117 1_3:-64598561 None:intergenic
AAGAAACAAAGACCAACAAC+TGG 0.466299 1_3:+64596929 Msa0133330:CDS
ATGTCTTATCACAAGCATCT+TGG 0.467546 1_3:-64598150 None:intergenic
TTCCCCTCCTTTGTGGTGAG+AGG 0.474043 1_3:-64596385 None:intergenic
AAGATGCTTTCAGAACTTGA+TGG 0.474203 1_3:+64597215 Msa0133330:three_prime_UTR
TAAACTTAGACTTGAGTAAG+AGG 0.482839 1_3:+64598233 Msa0133330:three_prime_UTR
ACTCATGTTGAACGCGCCAT+TGG 0.483109 1_3:+64596681 Msa0133330:CDS
TCTTGTCTGAGTAATGATTC+GGG 0.485143 1_3:-64596530 None:intergenic
GCCTATTGTTCGAGTAATTG+AGG 0.495600 1_3:+64598521 Msa0133330:three_prime_UTR
CACTTTGCTTATCACAGTTG+AGG 0.503180 1_3:-64598261 None:intergenic
TCTTCTACGCATTGCAAGCA+AGG 0.507249 1_3:-64597096 None:intergenic
AAGATAAACGAATCAAACTT+AGG 0.508452 1_3:+64596262 Msa0133330:CDS
CTTGTGTCCGCTGTACTTGG+TGG 0.509443 1_3:-64598110 None:intergenic
ATCAGCGTCGTTAACAGCCA+TGG 0.510244 1_3:-64595640 None:intergenic
TAAGTTTAGGTCTTCAGCTA+TGG 0.514869 1_3:-64598218 None:intergenic
CAGTGACAATGAAAGTGATA+AGG 0.519701 1_3:+64597763 Msa0133330:three_prime_UTR
CTTGATAATTTGACTACCAA+GGG 0.531078 1_3:+64596795 Msa0133330:CDS
TTCAATATAGTGTGCTCCAA+TGG 0.533972 1_3:-64596697 None:intergenic
CACCTTTGTCGATAATTCTG+AGG 0.534885 1_3:-64596987 None:intergenic
CACAAGCATCTTGGGCAGCA+AGG 0.535870 1_3:-64598141 None:intergenic
AGCAAGAAGGTATAGACCCT+TGG 0.540496 1_3:-64596811 None:intergenic
AAATACCTCTCTGGAAAGGA+AGG 0.542272 1_3:-64598552 None:intergenic
GCCGGACTATGAATTTGACC+AGG 0.542943 1_3:+64597703 Msa0133330:three_prime_UTR
CCTTGGTAGTCAAATTATCA+AGG 0.544621 1_3:-64596794 None:intergenic
CAGTAGTAAATAAGAGCTTA+CGG 0.549337 1_3:+64597468 Msa0133330:three_prime_UTR
AAGTGAGAATTGTATCCCTC+AGG 0.551342 1_3:+64596719 Msa0133330:CDS
AGCTGTAACTCTCTTAGCAC+TGG 0.557835 1_3:-64597425 None:intergenic
TTTCATAATATCACTCTGCA+GGG 0.559881 1_3:-64597363 None:intergenic
AACGAATCAAACTTAGGAGG+AGG 0.565962 1_3:+64596268 Msa0133330:CDS
TCATCATCTAAGAAGCGTAA+AGG 0.568425 1_3:+64598492 Msa0133330:three_prime_UTR
TTACCTCTCACCACAAAGGA+GGG 0.569474 1_3:+64596382 Msa0133330:CDS
CCTTGATAATTTGACTACCA+AGG 0.570856 1_3:+64596794 Msa0133330:CDS
GACTTATGATAATAGGACTC+AGG 0.574609 1_3:+64598890 Msa0133330:three_prime_UTR
TTGTGTCCGCTGTACTTGGT+GGG 0.576681 1_3:-64598109 None:intergenic
TAATCTCATAGGTCGTATGT+TGG 0.576899 1_3:+64598186 Msa0133330:three_prime_UTR
AGTTTAGTACACCTATGTCT+AGG 0.577720 1_3:+64597948 Msa0133330:three_prime_UTR
TGAAAGAAATTTGCAAAACC+GGG 0.580059 1_3:+64596413 Msa0133330:CDS
CTTCTTGCTATGATAGTTGG+TGG 0.583896 1_3:+64596825 Msa0133330:CDS
TATACGCATCTGTAAAGCCC+TGG 0.591076 1_3:-64597721 None:intergenic
AACTCTCTTAGCACTGGCAA+TGG 0.591095 1_3:-64597419 None:intergenic
GCTGTAGTGAAGGTGTTGTG+TGG 0.596201 1_3:+64597050 Msa0133330:three_prime_UTR
TAACAAATACCTCTCTGGAA+AGG 0.599447 1_3:-64598556 None:intergenic
CAGATGAAAATATTGTCAAG+AGG 0.600107 1_3:+64598011 Msa0133330:three_prime_UTR
ATTGCAAGCAAGGTTTGGGA+TGG 0.612588 1_3:-64597086 None:intergenic
ACAGCTTCTCATAGAACCAA+AGG 0.613174 1_3:+64597442 Msa0133330:three_prime_UTR
ATCCTCAGAATTATCGACAA+AGG 0.615555 1_3:+64596985 Msa0133330:three_prime_UTR
TCACTATTCAAGCATTCACT+GGG 0.615810 1_3:-64597836 None:intergenic
TTATCATGCTGCTGTAGTGA+AGG 0.615817 1_3:+64597040 Msa0133330:three_prime_UTR
CAATGAAAGTGATAAGGATG+AGG 0.617358 1_3:+64597769 Msa0133330:three_prime_UTR
TGAAATCCATATCATCCTGA+GGG 0.617603 1_3:-64596734 None:intergenic
TTTACCTCTCACCACAAAGG+AGG 0.624083 1_3:+64596381 Msa0133330:CDS
TTATTCCTTCCTTTCCAGAG+AGG 0.626159 1_3:+64598547 Msa0133330:three_prime_UTR
ATGCTTTCAGAACTTGATGG+AGG 0.626472 1_3:+64597218 Msa0133330:three_prime_UTR
CACCAACTATCATAGCAAGA+AGG 0.631132 1_3:-64596824 None:intergenic
ATAAACGAATCAAACTTAGG+AGG 0.631274 1_3:+64596265 Msa0133330:CDS
AAGAAATTTGCAAAACCGGG+TGG 0.633677 1_3:+64596416 Msa0133330:CDS
CCGGACTATGAATTTGACCA+GGG 0.634286 1_3:+64597704 Msa0133330:three_prime_UTR
TACCTCTCACCACAAAGGAG+GGG 0.641692 1_3:+64596383 Msa0133330:CDS
TGAGAAGTCTGCTATTGACA+TGG 0.642126 1_3:-64597270 None:intergenic
TCAATTACTCGAACAATAGG+CGG 0.645112 1_3:-64598519 None:intergenic
TCATCTAAGAAGCGTAAAGG+AGG 0.648277 1_3:+64598495 Msa0133330:three_prime_UTR
CTTTACAGATGCGTATATGG+AGG 0.653691 1_3:+64597727 Msa0133330:three_prime_UTR
TTGAAATCCATATCATCCTG+AGG 0.654948 1_3:-64596735 None:intergenic
AGGTTTACCTCTCACCACAA+AGG 0.659376 1_3:+64596378 Msa0133330:CDS
CCCATACCCACCAAGTACAG+CGG 0.670328 1_3:+64598103 Msa0133330:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTCAAAAAAAAAAAAAAAAA+CGG - chr1_3:64595902-64595921 None:intergenic 10.0%
!! CAATATTTAATTGTATAAAT+TGG - chr1_3:64598338-64598357 None:intergenic 10.0%
!! TATTTAGAAAAATAAAATCA+TGG - chr1_3:64598650-64598669 None:intergenic 10.0%
!! GTTATTATTATGTAATTCAA+TGG + chr1_3:64595693-64595712 Msa0133330:intron 15.0%
!! GGAATATAATAATACAATAA+AGG - chr1_3:64596568-64596587 None:intergenic 15.0%
!! TATTTAATTGTATAAATTGG+TGG - chr1_3:64598335-64598354 None:intergenic 15.0%
!! TACAATTAAATATTGTTGTA+TGG + chr1_3:64598343-64598362 Msa0133330:intron 15.0%
!! AATTAAACTATATTTCCCTT+AGG + chr1_3:64596166-64596185 Msa0133330:intron 20.0%
!! TACATTAATTAAAATGATGC+AGG + chr1_3:64596347-64596366 Msa0133330:intron 20.0%
!! AAGGAAAAAAGAATTCATTA+GGG - chr1_3:64596483-64596502 None:intergenic 20.0%
!! AAAGGAAAAAAGAATTCATT+AGG - chr1_3:64596484-64596503 None:intergenic 20.0%
!!! AATATGAACCTTTTTAAGTT+TGG - chr1_3:64596768-64596787 None:intergenic 20.0%
!!! GATATTAGGTAGTTTTTTTT+AGG + chr1_3:64598766-64598785 Msa0133330:intron 20.0%
!!! GTATGAAATTTTGGAATTAA+TGG - chr1_3:64599032-64599051 None:intergenic 20.0%
! AGGAGTAGTTATAATTAAGA+GGG + chr1_3:64595746-64595765 Msa0133330:intron 25.0%
! ACTTCTTGTGTAAAAAAACA+TGG + chr1_3:64595825-64595844 Msa0133330:intron 25.0%
! AAAAAAACATGGCAAAATTG+TGG + chr1_3:64595836-64595855 Msa0133330:intron 25.0%
! AAAAAACATGGCAAAATTGT+GGG + chr1_3:64595837-64595856 Msa0133330:intron 25.0%
! TTAAATCATTTGTTCCAGAA+TGG - chr1_3:64596067-64596086 None:intergenic 25.0%
!! TAAGTTTGATTCGTTTATCT+TGG - chr1_3:64596263-64596282 None:intergenic 25.0%
! AAGATAAACGAATCAAACTT+AGG + chr1_3:64596262-64596281 Msa0133330:CDS 25.0%
!!! AATCGTCATTTTTGTCTTTT+TGG + chr1_3:64596292-64596311 Msa0133330:intron 25.0%
!!! ATCGTCATTTTTGTCTTTTT+GGG + chr1_3:64596293-64596312 Msa0133330:intron 25.0%
! ATTAATTAAAATGATGCAGG+TGG + chr1_3:64596350-64596369 Msa0133330:intron 25.0%
! TTGAAAGAAATTTGCAAAAC+CGG + chr1_3:64596412-64596431 Msa0133330:CDS 25.0%
!! TGTTAAATGATTTAGTCTTC+AGG + chr1_3:64596452-64596471 Msa0133330:intron 25.0%
! TGTAGACAAGAAAAGAAAAA+AGG - chr1_3:64596502-64596521 None:intergenic 25.0%
!!! TTTTTTCTTTTCTTGTCTAC+AGG + chr1_3:64596501-64596520 Msa0133330:intron 25.0%
! ACCAAATACCAAACTTAAAA+AGG + chr1_3:64596757-64596776 Msa0133330:CDS 25.0%
!!! ACCTTTTTAAGTTTGGTATT+TGG - chr1_3:64596761-64596780 None:intergenic 25.0%
! TTCTCACTAAAACTGATAAT+TGG + chr1_3:64597287-64597306 Msa0133330:three_prime_UTR 25.0%
!!! CTAAAACTGATAATTGGTTT+TGG + chr1_3:64597293-64597312 Msa0133330:three_prime_UTR 25.0%
! TATATTGTCTGTTGAGAAAA+AGG + chr1_3:64597388-64597407 Msa0133330:three_prime_UTR 25.0%
! GAACTGTGATACAATAAATT+TGG + chr1_3:64597868-64597887 Msa0133330:three_prime_UTR 25.0%
! TTTGTTACATTCCTCAATTA+TGG + chr1_3:64598572-64598591 Msa0133330:intron 25.0%
! AAAGATGCAAATGACTTAAA+GGG - chr1_3:64598718-64598737 None:intergenic 25.0%
! CATACTCAGATATAAAAACA+TGG - chr1_3:64598815-64598834 None:intergenic 25.0%
!!! ATTGATGGACTTATGATAAT+AGG + chr1_3:64598883-64598902 Msa0133330:three_prime_UTR 25.0%
!!! TTGAAATGGTTTTGCATTTT+TGG + chr1_3:64598941-64598960 Msa0133330:three_prime_UTR 25.0%
! CAGTGTAAAAACTCATAATT+TGG - chr1_3:64598977-64598996 None:intergenic 25.0%
!! AATTCTCTTCTGTTTTATAG+TGG + chr1_3:64599062-64599081 Msa0133330:three_prime_UTR 25.0%
! ACAAAGCTTCAATTAATTTC+AGG + chr1_3:64599085-64599104 Msa0133330:three_prime_UTR 25.0%
GAGGAGTAGTTATAATTAAG+AGG + chr1_3:64595745-64595764 Msa0133330:intron 30.0%
GGAGTAGTTATAATTAAGAG+GGG + chr1_3:64595747-64595766 Msa0133330:intron 30.0%
! TTTACACAAGAAGTTGTTTC+CGG - chr1_3:64595819-64595838 None:intergenic 30.0%
AAAAACATGGCAAAATTGTG+GGG + chr1_3:64595838-64595857 Msa0133330:intron 30.0%
ACAAAAGCAAAAGCAAAAAC+AGG - chr1_3:64596118-64596137 None:intergenic 30.0%
AAACTATATTTCCCTTAGGT+TGG + chr1_3:64596170-64596189 Msa0133330:intron 30.0%
ATAAACGAATCAAACTTAGG+AGG + chr1_3:64596265-64596284 Msa0133330:CDS 30.0%
!!! ATTTTTGTCTTTTTGGGTGT+TGG + chr1_3:64596299-64596318 Msa0133330:intron 30.0%
TGAAAGAAATTTGCAAAACC+GGG + chr1_3:64596413-64596432 Msa0133330:CDS 30.0%
ATCTTGTCTGAGTAATGATT+CGG - chr1_3:64596534-64596553 None:intergenic 30.0%
! CTTGATAATTTGACTACCAA+GGG + chr1_3:64596795-64596814 Msa0133330:CDS 30.0%
TAAAATTGTGACTCAGATCT+AGG - chr1_3:64597023-64597042 None:intergenic 30.0%
TATTCACTTGTCTGATTAGA+TGG - chr1_3:64597184-64597203 None:intergenic 30.0%
TTTCATAATATCACTCTGCA+GGG - chr1_3:64597366-64597385 None:intergenic 30.0%
TTATTTACTACTGTAGCCTT+TGG - chr1_3:64597461-64597480 None:intergenic 30.0%
CAGTAGTAAATAAGAGCTTA+CGG + chr1_3:64597468-64597487 Msa0133330:three_prime_UTR 30.0%
! TACATCATGCATGCTTTTAT+TGG - chr1_3:64597597-64597616 None:intergenic 30.0%
!! AATTATTCAAGACTTGTTGC+CGG + chr1_3:64597685-64597704 Msa0133330:three_prime_UTR 30.0%
!! TCTTGGCATTGGTTATTATA+TGG - chr1_3:64597914-64597933 None:intergenic 30.0%
! CTCTTGACAATATTTTCATC+TGG - chr1_3:64598013-64598032 None:intergenic 30.0%
CAGATGAAAATATTGTCAAG+AGG + chr1_3:64598011-64598030 Msa0133330:three_prime_UTR 30.0%
ATGCTTGCATATAATCTCAT+AGG + chr1_3:64598175-64598194 Msa0133330:three_prime_UTR 30.0%
TCTTACTCAAGTCTAAGTTT+AGG - chr1_3:64598234-64598253 None:intergenic 30.0%
TAAACTTAGACTTGAGTAAG+AGG + chr1_3:64598233-64598252 Msa0133330:three_prime_UTR 30.0%
AAGCAAAGTGAAGACATAAA+AGG + chr1_3:64598274-64598293 Msa0133330:three_prime_UTR 30.0%
! ATTGTATAAATTGGTGGTAG+CGG - chr1_3:64598329-64598348 None:intergenic 30.0%
ATATTGTTGTATGGTCATAC+TGG + chr1_3:64598352-64598371 Msa0133330:intron 30.0%
! ATGACAGTTAAGTAGTGTTT+TGG + chr1_3:64598396-64598415 Msa0133330:intron 30.0%
CTTTACTCATTTGCATATGT+GGG + chr1_3:64598672-64598691 Msa0133330:intron 30.0%
CAAAGATGCAAATGACTTAA+AGG - chr1_3:64598719-64598738 None:intergenic 30.0%
AGATGCGTAACAAAGATATT+AGG + chr1_3:64598752-64598771 Msa0133330:intron 30.0%
!! CTCTAAGGAGTATGAAATTT+TGG - chr1_3:64599041-64599060 None:intergenic 30.0%
TATAATTAAGAGGGGAGCTT+TGG + chr1_3:64595755-64595774 Msa0133330:intron 35.0%
AAAACATGGCAAAATTGTGG+GGG + chr1_3:64595839-64595858 Msa0133330:intron 35.0%
TGCACAAGTGAATTAGATAC+AGG - chr1_3:64595973-64595992 None:intergenic 35.0%
TCTAATTCACTTGTGCATCA+TGG + chr1_3:64595976-64595995 Msa0133330:intron 35.0%
CTAATTCACTTGTGCATCAT+GGG + chr1_3:64595977-64595996 Msa0133330:intron 35.0%
!!! ACCTCTTTTTTGTTCCATTC+TGG + chr1_3:64596050-64596069 Msa0133330:intron 35.0%
TCCAGAATGGAACAAAAAAG+AGG - chr1_3:64596054-64596073 None:intergenic 35.0%
AAATGATGCAGGTGGTTATT+AGG + chr1_3:64596358-64596377 Msa0133330:intron 35.0%
TCTTGTCTGAGTAATGATTC+GGG - chr1_3:64596533-64596552 None:intergenic 35.0%
TTACTCAGACAAGATGATGT+AGG + chr1_3:64596539-64596558 Msa0133330:intron 35.0%
AGAACAAAGCAAGTAACTCT+AGG - chr1_3:64596589-64596608 None:intergenic 35.0%
TTCAATATAGTGTGCTCCAA+TGG - chr1_3:64596700-64596719 None:intergenic 35.0%
TGAAATCCATATCATCCTGA+GGG - chr1_3:64596737-64596756 None:intergenic 35.0%
TTGAAATCCATATCATCCTG+AGG - chr1_3:64596738-64596757 None:intergenic 35.0%
! CCTTGGTAGTCAAATTATCA+AGG - chr1_3:64596797-64596816 None:intergenic 35.0%
! CCTTGATAATTTGACTACCA+AGG + chr1_3:64596794-64596813 Msa0133330:CDS 35.0%
! TACCTTCTTGCTATGATAGT+TGG + chr1_3:64596822-64596841 Msa0133330:CDS 35.0%
AAGAAACAAAGACCAACAAC+TGG + chr1_3:64596929-64596948 Msa0133330:CDS 35.0%
ATCCTCAGAATTATCGACAA+AGG + chr1_3:64596985-64597004 Msa0133330:three_prime_UTR 35.0%
AAGATGCTTTCAGAACTTGA+TGG + chr1_3:64597215-64597234 Msa0133330:three_prime_UTR 35.0%
CTTTCATAATATCACTCTGC+AGG - chr1_3:64597367-64597386 None:intergenic 35.0%
AGAATCTGCAATAGAGACTT+TGG - chr1_3:64597554-64597573 None:intergenic 35.0%
CTTCTTCAATATGCTTCTTC+TGG - chr1_3:64597625-64597644 None:intergenic 35.0%
CAGTGACAATGAAAGTGATA+AGG + chr1_3:64597763-64597782 Msa0133330:three_prime_UTR 35.0%
CAATGAAAGTGATAAGGATG+AGG + chr1_3:64597769-64597788 Msa0133330:three_prime_UTR 35.0%
TCACTATTCAAGCATTCACT+GGG - chr1_3:64597839-64597858 None:intergenic 35.0%
ATCACTATTCAAGCATTCAC+TGG - chr1_3:64597840-64597859 None:intergenic 35.0%
AGTTTAGTACACCTATGTCT+AGG + chr1_3:64597948-64597967 Msa0133330:three_prime_UTR 35.0%
! TCACAGTTTTTCCTAGACAT+AGG - chr1_3:64597962-64597981 None:intergenic 35.0%
CAAAAGTTGGATTCATCTCT+TGG - chr1_3:64598070-64598089 None:intergenic 35.0%
!!! TTTTTTCTCCTCGCAAAAGT+TGG - chr1_3:64598083-64598102 None:intergenic 35.0%
TGTCTTATCACAAGCATCTT+GGG - chr1_3:64598152-64598171 None:intergenic 35.0%
ATGTCTTATCACAAGCATCT+TGG - chr1_3:64598153-64598172 None:intergenic 35.0%
TAATCTCATAGGTCGTATGT+TGG + chr1_3:64598186-64598205 Msa0133330:three_prime_UTR 35.0%
TAAGTTTAGGTCTTCAGCTA+TGG - chr1_3:64598221-64598240 None:intergenic 35.0%
! TCATCATCTAAGAAGCGTAA+AGG + chr1_3:64598492-64598511 Msa0133330:three_prime_UTR 35.0%
TCAATTACTCGAACAATAGG+CGG - chr1_3:64598522-64598541 None:intergenic 35.0%
TCCTCAATTACTCGAACAAT+AGG - chr1_3:64598525-64598544 None:intergenic 35.0%
! TAACAAATACCTCTCTGGAA+AGG - chr1_3:64598559-64598578 None:intergenic 35.0%
GAATGTAACAAATACCTCTC+TGG - chr1_3:64598564-64598583 None:intergenic 35.0%
TTCGTATGCTACCATAATTG+AGG - chr1_3:64598586-64598605 None:intergenic 35.0%
GCTTTACTCATTTGCATATG+TGG + chr1_3:64598671-64598690 Msa0133330:intron 35.0%
GGAAAGACTGAAGATATTGA+TGG + chr1_3:64598868-64598887 Msa0133330:three_prime_UTR 35.0%
GACTTATGATAATAGGACTC+AGG + chr1_3:64598890-64598909 Msa0133330:three_prime_UTR 35.0%
AACAGAAGAGAATTGCTCTA+AGG - chr1_3:64599056-64599075 None:intergenic 35.0%
TAGAGATGTGTTCGATTACG+AGG + chr1_3:64595726-64595745 Msa0133330:intron 40.0%
!!! TCACGTGACTTTTCAAGTTC+CGG + chr1_3:64595797-64595816 Msa0133330:intron 40.0%
AAACATGGCAAAATTGTGGG+GGG + chr1_3:64595840-64595859 Msa0133330:intron 40.0%
ACTAAGCTGATCCAACCTAA+GGG - chr1_3:64596184-64596203 None:intergenic 40.0%
AACTAAGCTGATCCAACCTA+AGG - chr1_3:64596185-64596204 None:intergenic 40.0%
AACGAATCAAACTTAGGAGG+AGG + chr1_3:64596268-64596287 Msa0133330:CDS 40.0%
AAGAAATTTGCAAAACCGGG+TGG + chr1_3:64596416-64596435 Msa0133330:CDS 40.0%
AAGTGAGAATTGTATCCCTC+AGG + chr1_3:64596719-64596738 Msa0133330:CDS 40.0%
TTGTATCCCTCAGGATGATA+TGG + chr1_3:64596728-64596747 Msa0133330:CDS 40.0%
CACCAACTATCATAGCAAGA+AGG - chr1_3:64596827-64596846 None:intergenic 40.0%
! CTTCTTGCTATGATAGTTGG+TGG + chr1_3:64596825-64596844 Msa0133330:CDS 40.0%
CACCTTTGTCGATAATTCTG+AGG - chr1_3:64596990-64597009 None:intergenic 40.0%
TTATCATGCTGCTGTAGTGA+AGG + chr1_3:64597040-64597059 Msa0133330:three_prime_UTR 40.0%
GCAATGCGTAGAAGACTTAA+AGG + chr1_3:64597104-64597123 Msa0133330:three_prime_UTR 40.0%
ATGCTTTCAGAACTTGATGG+AGG + chr1_3:64597218-64597237 Msa0133330:three_prime_UTR 40.0%
!! ATTGACATGGCTTTGGCTAA+TGG - chr1_3:64597260-64597279 None:intergenic 40.0%
TGAGAAGTCTGCTATTGACA+TGG - chr1_3:64597273-64597292 None:intergenic 40.0%
ACAGCTTCTCATAGAACCAA+AGG + chr1_3:64597442-64597461 Msa0133330:three_prime_UTR 40.0%
TCTCTTTGAGTGCAGTGATA+TGG + chr1_3:64597520-64597539 Msa0133330:three_prime_UTR 40.0%
CTTTACAGATGCGTATATGG+AGG + chr1_3:64597727-64597746 Msa0133330:three_prime_UTR 40.0%
CTGTGATTTCTTCTTGGCAT+TGG - chr1_3:64597925-64597944 None:intergenic 40.0%
ACTGCTCTGTGATTTCTTCT+TGG - chr1_3:64597931-64597950 None:intergenic 40.0%
! AGATGAATCCAACTTTTGCG+AGG + chr1_3:64598072-64598091 Msa0133330:three_prime_UTR 40.0%
CACTTTGCTTATCACAGTTG+AGG - chr1_3:64598264-64598283 None:intergenic 40.0%
! TCATCTAAGAAGCGTAAAGG+AGG + chr1_3:64598495-64598514 Msa0133330:three_prime_UTR 40.0%
GCCTATTGTTCGAGTAATTG+AGG + chr1_3:64598521-64598540 Msa0133330:three_prime_UTR 40.0%
TTATTCCTTCCTTTCCAGAG+AGG + chr1_3:64598547-64598566 Msa0133330:three_prime_UTR 40.0%
! AAATACCTCTCTGGAAAGGA+AGG - chr1_3:64598555-64598574 None:intergenic 40.0%
AAATGACTTAAAGGGCAACC+CGG - chr1_3:64598710-64598729 None:intergenic 40.0%
! GTATGCGTAACTCTTGCTTT+TGG + chr1_3:64598830-64598849 Msa0133330:intron 40.0%
ATAATAGGACTCAGGCTTTC+AGG + chr1_3:64598898-64598917 Msa0133330:three_prime_UTR 40.0%
CATTTCAAAATGCTCTTCGC+TGG - chr1_3:64598929-64598948 None:intergenic 40.0%
!!! CAGCGAAGAGCATTTTGAAA+TGG + chr1_3:64598927-64598946 Msa0133330:three_prime_UTR 40.0%
TAGTTCCAGCTTCTCAGCTT+CGG + chr1_3:64595938-64595957 Msa0133330:intron 45.0%
TATTTCCGAAGCTGAGAAGC+TGG - chr1_3:64595946-64595965 None:intergenic 45.0%
AGGTTTACCTCTCACCACAA+AGG + chr1_3:64596378-64596397 Msa0133330:CDS 45.0%
TTTACCTCTCACCACAAAGG+AGG + chr1_3:64596381-64596400 Msa0133330:CDS 45.0%
TTACCTCTCACCACAAAGGA+GGG + chr1_3:64596382-64596401 Msa0133330:CDS 45.0%
! AGCAAGAAGGTATAGACCCT+TGG - chr1_3:64596814-64596833 None:intergenic 45.0%
GTTTGCGAGTTTCCAGTTGT+TGG - chr1_3:64596944-64596963 None:intergenic 45.0%
ATTGCAAGCAAGGTTTGGGA+TGG - chr1_3:64597089-64597108 None:intergenic 45.0%
ACGCATTGCAAGCAAGGTTT+GGG - chr1_3:64597093-64597112 None:intergenic 45.0%
TACGCATTGCAAGCAAGGTT+TGG - chr1_3:64597094-64597113 None:intergenic 45.0%
TCTTCTACGCATTGCAAGCA+AGG - chr1_3:64597099-64597118 None:intergenic 45.0%
! TTTTTGCAAGGTTGCAGCTG+AGG - chr1_3:64597146-64597165 None:intergenic 45.0%
CAGCTGCAACCTTGCAAAAA+CGG + chr1_3:64597146-64597165 Msa0133330:three_prime_UTR 45.0%
TGCAACCTTGCAAAAACGGA+TGG + chr1_3:64597150-64597169 Msa0133330:three_prime_UTR 45.0%
GCAACCTTGCAAAAACGGAT+GGG + chr1_3:64597151-64597170 Msa0133330:three_prime_UTR 45.0%
CAACCTTGCAAAAACGGATG+GGG + chr1_3:64597152-64597171 Msa0133330:three_prime_UTR 45.0%
GTCTGCTATTGACATGGCTT+TGG - chr1_3:64597267-64597286 None:intergenic 45.0%
!! AACTCTCTTAGCACTGGCAA+TGG - chr1_3:64597422-64597441 None:intergenic 45.0%
AGCTGTAACTCTCTTAGCAC+TGG - chr1_3:64597428-64597447 None:intergenic 45.0%
CCCTGGTCAAATTCATAGTC+CGG - chr1_3:64597707-64597726 None:intergenic 45.0%
CCGGACTATGAATTTGACCA+GGG + chr1_3:64597704-64597723 Msa0133330:three_prime_UTR 45.0%
TATACGCATCTGTAAAGCCC+TGG - chr1_3:64597724-64597743 None:intergenic 45.0%
GGGCTTTACAGATGCGTATA+TGG + chr1_3:64597724-64597743 Msa0133330:three_prime_UTR 45.0%
ATAAAGCTCCCCCATACGCA+TGG - chr1_3:64595875-64595894 None:intergenic 50.0%
TACCTCTCACCACAAAGGAG+GGG + chr1_3:64596383-64596402 Msa0133330:CDS 50.0%
CAACTTCTTCCCCTCCTTTG+TGG - chr1_3:64596395-64596414 None:intergenic 50.0%
ACTCATGTTGAACGCGCCAT+TGG + chr1_3:64596681-64596700 Msa0133330:CDS 50.0%
!! GCTGTAGTGAAGGTGTTGTG+TGG + chr1_3:64597050-64597069 Msa0133330:three_prime_UTR 50.0%
CTCTGCAGGGATTTCACTTC+AGG - chr1_3:64597353-64597372 None:intergenic 50.0%
GCCGGACTATGAATTTGACC+AGG + chr1_3:64597703-64597722 Msa0133330:three_prime_UTR 50.0%
TTGTGTCCGCTGTACTTGGT+GGG - chr1_3:64598112-64598131 None:intergenic 50.0%
GTTCTTGTGTCCGCTGTACT+TGG - chr1_3:64598116-64598135 None:intergenic 50.0%
CATCTTGGGCAGCAAGGTAT+TGG - chr1_3:64598138-64598157 None:intergenic 50.0%
! GGTAGCGGTCATAGCAGTAA+CGG - chr1_3:64598314-64598333 None:intergenic 50.0%
!! CTTGCTTTTGGACACTGCAG+TGG + chr1_3:64598842-64598861 Msa0133330:intron 50.0%
! TTTTGGACACTGCAGTGGCA+TGG + chr1_3:64598847-64598866 Msa0133330:intron 50.0%
! TAAGAGGGGAGCTTTGGTGC+AGG + chr1_3:64595761-64595780 Msa0133330:intron 55.0%
! AAGAGGGGAGCTTTGGTGCA+GGG + chr1_3:64595762-64595781 Msa0133330:intron 55.0%
CAAAATTGTGGGGGGCTTCC+CGG + chr1_3:64595848-64595867 Msa0133330:intron 55.0%
TTCCCCTCCTTTGTGGTGAG+AGG - chr1_3:64596388-64596407 None:intergenic 55.0%
! CAGATGAAGCACTCACCACC+CGG - chr1_3:64596434-64596453 None:intergenic 55.0%
CAAGGTTGCAGCTGAGGCAT+CGG - chr1_3:64597140-64597159 None:intergenic 55.0%
!! CGTCCCCATCCGTTTTTGCA+AGG - chr1_3:64597158-64597177 None:intergenic 55.0%
! CCGCTGTACTTGGTGGGTAT+GGG - chr1_3:64598106-64598125 None:intergenic 55.0%
CCCATACCCACCAAGTACAG+CGG + chr1_3:64598103-64598122 Msa0133330:three_prime_UTR 55.0%
! TCCGCTGTACTTGGTGGGTA+TGG - chr1_3:64598107-64598126 None:intergenic 55.0%
CTTGTGTCCGCTGTACTTGG+TGG - chr1_3:64598113-64598132 None:intergenic 55.0%
CACAAGCATCTTGGGCAGCA+AGG - chr1_3:64598144-64598163 None:intergenic 55.0%
! ATGTGGGAGCTCTAGTGCAC+CGG + chr1_3:64598688-64598707 Msa0133330:intron 55.0%
GCTTCCCGGACCATGCGTAT+GGG + chr1_3:64595862-64595881 Msa0133330:intron 60.0%
CTTCCCGGACCATGCGTATG+GGG + chr1_3:64595863-64595882 Msa0133330:intron 60.0%
TTCCCGGACCATGCGTATGG+GGG + chr1_3:64595864-64595883 Msa0133330:intron 60.0%
! TGTGGGAGCTCTAGTGCACC+GGG + chr1_3:64598689-64598708 Msa0133330:intron 60.0%
GGCTTCCCGGACCATGCGTA+TGG + chr1_3:64595861-64595880 Msa0133330:intron 65.0%
CTCCCCCATACGCATGGTCC+GGG - chr1_3:64595869-64595888 None:intergenic 65.0%
GCTCCCCCATACGCATGGTC+CGG - chr1_3:64595870-64595889 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 64595642 64599129 64595642 ID=Msa0133330;Name=Msa0133330
chr1_3 mRNA 64595642 64599129 64595642 ID=Msa0133330-mRNA-1;Parent=Msa0133330;Name=Msa0133330-mRNA-1;_AED=0.03;_eAED=0.03;_QI=0|0.83|0.71|0.85|1|0.85|7|1677|181
chr1_3 exon 64595642 64595703 64595642 ID=Msa0133330-mRNA-1:exon:21754;Parent=Msa0133330-mRNA-1
chr1_3 exon 64596224 64596289 64596224 ID=Msa0133330-mRNA-1:exon:21755;Parent=Msa0133330-mRNA-1
chr1_3 exon 64596369 64596437 64596369 ID=Msa0133330-mRNA-1:exon:21756;Parent=Msa0133330-mRNA-1
chr1_3 exon 64596523 64596556 64596523 ID=Msa0133330-mRNA-1:exon:21757;Parent=Msa0133330-mRNA-1
chr1_3 exon 64596660 64598295 64596660 ID=Msa0133330-mRNA-1:exon:21758;Parent=Msa0133330-mRNA-1
chr1_3 exon 64598481 64598568 64598481 ID=Msa0133330-mRNA-1:exon:21759;Parent=Msa0133330-mRNA-1
chr1_3 exon 64598862 64599129 64598862 ID=Msa0133330-mRNA-1:exon:21760;Parent=Msa0133330-mRNA-1
chr1_3 CDS 64595642 64595703 64595642 ID=Msa0133330-mRNA-1:cds;Parent=Msa0133330-mRNA-1
chr1_3 CDS 64596224 64596289 64596224 ID=Msa0133330-mRNA-1:cds;Parent=Msa0133330-mRNA-1
chr1_3 CDS 64596369 64596437 64596369 ID=Msa0133330-mRNA-1:cds;Parent=Msa0133330-mRNA-1
chr1_3 CDS 64596523 64596556 64596523 ID=Msa0133330-mRNA-1:cds;Parent=Msa0133330-mRNA-1
chr1_3 CDS 64596660 64596974 64596660 ID=Msa0133330-mRNA-1:cds;Parent=Msa0133330-mRNA-1
chr1_3 three_prime_UTR 64596975 64598295 64596975 ID=Msa0133330-mRNA-1:three_prime_utr;Parent=Msa0133330-mRNA-1
chr1_3 three_prime_UTR 64598481 64598568 64598481 ID=Msa0133330-mRNA-1:three_prime_utr;Parent=Msa0133330-mRNA-1
chr1_3 three_prime_UTR 64598862 64599129 64598862 ID=Msa0133330-mRNA-1:three_prime_utr;Parent=Msa0133330-mRNA-1
Gene Sequence

>Msa0133330

ATGGCTGTTAACGACGCTGATCAATCTCACAAAGCTCACAGTACTGTTACTGTTATTATTATAACAGATATCTGTTCTATTTTAAGAGATGCAAAGCGCCAAGATAAACGAATCAAACTTAGGAGGAGGTGGTTATTAGGTTTACCTCTCACCACAAAGGAGGGGAAGAAGTTGAAAGAAATTTGCAAAACCGGGTGGTATTCACCCGAATCATTACTCAGACAAGATGATCTATTTTATGAGTCAGTGAGAACTCATGTTGAACGCGCCATTGGAGCACACTATATTGAAAGTGAGAATTGTATCCCTCAGGATGATATGGATTTCAACCAAATACCAAACTTAAAAAGGTTCATATTATCATGCCTTGATAATTTGACTACCAAGGGTCTATACCTTCTTGCTATGATAGTTGGTGGTGACACAATCAAGTATGAAAGAACTCGAAGTAATTTGAAAAAGATTATCAAGAGTTCTCTTTCAAGCGTTCTGAGTAGCGAAAGAAACAAAGACCAACAACTGGAAACTCGCAAACAGCTTTTTTAA
Protein sequence

>Msa0133330

MAVNDADQSHKAHSTVTVIIITDICSILRDAKRQDKRIKLRRRWLLGLPLTTKEGKKLKEICKTGWYSPESLLRQDDLFYESVRTHVERAIGAHYIESENCIPQDDMDFNQIPNLKRFILSCLDNLTTKGLYLLAMIVGGDTIKYERTRSNLKKIIKSSLSSVLSSERNKDQQLETRKQLF*