AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0133300

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0133300 MtrunA17_Chr4g0033801 93.750 160 10 0 22 181 6 165 2.36e-98 297
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0133300 AT5G40520.2 39.041 146 79 4 17 160 7 144 1.44e-23 97.1

Find 117 sgRNAs with CRISPR-Local

Find 208 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTAAGGAGTATGAAATTT+TGG 0.141746 1_3:-64570358 None:intergenic
ACCAAATACCAAACTTAAAA+AGG 0.178603 1_3:+64568077 Msa0133300:CDS
ATAATAGGACTCAGGCTTTC+AGG 0.199003 1_3:+64570218 Msa0133300:three_prime_UTR
CTTCTTCAATATGCTTCTTC+TGG 0.209658 1_3:-64568942 None:intergenic
TTCTCACTAAAACTGATAAT+TGG 0.229390 1_3:+64568607 Msa0133300:three_prime_UTR
GAACTGTGATACAATAAATT+TGG 0.238520 1_3:+64569188 Msa0133300:three_prime_UTR
TCTTGGCATTGGTTATTATA+TGG 0.244429 1_3:-64569231 None:intergenic
TATTCACTTGTCTGATTAGA+TGG 0.250819 1_3:-64568501 None:intergenic
TATATTGTCTGTTGAGAAAA+AGG 0.250997 1_3:+64568708 Msa0133300:three_prime_UTR
ACTGCTCTGTGATTTCTTCT+TGG 0.263092 1_3:-64569248 None:intergenic
AAATGATGCAGGTGGTTATT+AGG 0.268573 1_3:+64567678 Msa0133300:intron
GCAATGCGTAGAAGACTTAA+AGG 0.269672 1_3:+64568424 Msa0133300:three_prime_UTR
ACAAAGCTTCAATTAATTTC+AGG 0.280363 1_3:+64570405 Msa0133300:three_prime_UTR
TCTCTTTGAGTGCAGTGATA+TGG 0.305905 1_3:+64568840 Msa0133300:three_prime_UTR
CAACTTCTTCCCCTCCTTTG+TGG 0.306669 1_3:-64567712 None:intergenic
CTAAAACTGATAATTGGTTT+TGG 0.323266 1_3:+64568613 Msa0133300:three_prime_UTR
TAAGTTTGATTCGTTTATCT+TGG 0.323820 1_3:-64567580 None:intergenic
ATTGACATGGCTTTGGCTAA+TGG 0.329725 1_3:-64568577 None:intergenic
TTGAAAGAAATTTGCAAAAC+CGG 0.334175 1_3:+64567732 Msa0133300:CDS
ATTGATGGACTTATGATAAT+AGG 0.335665 1_3:+64570203 Msa0133300:three_prime_UTR
CAAAAGTTGGATTCATCTCT+TGG 0.336309 1_3:-64569387 None:intergenic
TCCGCTGTACTTGGTGGGTA+TGG 0.339166 1_3:-64569424 None:intergenic
CTCTGCAGGGATTTCACTTC+AGG 0.340422 1_3:-64568670 None:intergenic
TACGCATTGCAAGCAAGGTT+TGG 0.340862 1_3:-64568411 None:intergenic
CTGTGATTTCTTCTTGGCAT+TGG 0.350204 1_3:-64569242 None:intergenic
TTGTATCCCTCAGGATGATA+TGG 0.350338 1_3:+64568048 Msa0133300:CDS
GGGCTTTACAGATGCGTATA+TGG 0.364505 1_3:+64569044 Msa0133300:three_prime_UTR
TTATTTACTACTGTAGCCTT+TGG 0.367086 1_3:-64568778 None:intergenic
ACGCATTGCAAGCAAGGTTT+GGG 0.377969 1_3:-64568410 None:intergenic
AAGCAAAGTGAAGACATAAA+AGG 0.380673 1_3:+64569594 Msa0133300:three_prime_UTR
GTTTGCGAGTTTCCAGTTGT+TGG 0.386231 1_3:-64568261 None:intergenic
GTTCTTGTGTCCGCTGTACT+TGG 0.390736 1_3:-64569433 None:intergenic
ATCATTGGCGCTAAAATATT+CGG 0.394849 1_3:-64570433 None:intergenic
TACCTTCTTGCTATGATAGT+TGG 0.398012 1_3:+64568142 Msa0133300:CDS
CATCTTGGGCAGCAAGGTAT+TGG 0.398219 1_3:-64569455 None:intergenic
GTCTGCTATTGACATGGCTT+TGG 0.401689 1_3:-64568584 None:intergenic
ATCTTGTCTGAGTAATGATT+CGG 0.404131 1_3:-64567851 None:intergenic
AATTATTCAAGACTTGTTGC+CGG 0.419014 1_3:+64569005 Msa0133300:three_prime_UTR
AACAGAAGAGAATTGCTCTA+AGG 0.420044 1_3:-64570373 None:intergenic
CATTTCAAAATGCTCTTCGC+TGG 0.420868 1_3:-64570246 None:intergenic
AGAATCTGCAATAGAGACTT+TGG 0.423522 1_3:-64568871 None:intergenic
TAAAATTGTGACTCAGATCT+AGG 0.438338 1_3:-64568340 None:intergenic
TCCTCAATTACTCGAACAAT+AGG 0.442380 1_3:-64569842 None:intergenic
TCTTACTCAAGTCTAAGTTT+AGG 0.443346 1_3:-64569551 None:intergenic
TGTCTTATCACAAGCATCTT+GGG 0.446020 1_3:-64569469 None:intergenic
CAAGGTTGCAGCTGAGGCAT+CGG 0.452977 1_3:-64568457 None:intergenic
CCGCTGTACTTGGTGGGTAT+GGG 0.453476 1_3:-64569423 None:intergenic
GGAAAGACTGAAGATATTGA+TGG 0.454052 1_3:+64570188 Msa0133300:three_prime_UTR
CTTTCATAATATCACTCTGC+AGG 0.457564 1_3:-64568684 None:intergenic
ATGCTTGCATATAATCTCAT+AGG 0.457983 1_3:+64569495 Msa0133300:three_prime_UTR
CCCTGGTCAAATTCATAGTC+CGG 0.460102 1_3:-64569024 None:intergenic
ATCACTATTCAAGCATTCAC+TGG 0.466056 1_3:-64569157 None:intergenic
GAATGTAACAAATACCTCTC+TGG 0.466117 1_3:-64569881 None:intergenic
AAGAAACAAAGACCAACAAC+TGG 0.466299 1_3:+64568249 Msa0133300:CDS
ATGTCTTATCACAAGCATCT+TGG 0.467546 1_3:-64569470 None:intergenic
TTCCCCTCCTTTGTGGTGAG+AGG 0.474043 1_3:-64567705 None:intergenic
AAGATGCTTTCAGAACTTGA+TGG 0.474203 1_3:+64568535 Msa0133300:three_prime_UTR
TAAACTTAGACTTGAGTAAG+AGG 0.482839 1_3:+64569553 Msa0133300:three_prime_UTR
ACTCATGTTGAACGCGCCAT+TGG 0.483109 1_3:+64568001 Msa0133300:CDS
TCTTGTCTGAGTAATGATTC+GGG 0.485143 1_3:-64567850 None:intergenic
GCCTATTGTTCGAGTAATTG+AGG 0.495600 1_3:+64569841 Msa0133300:three_prime_UTR
CACTTTGCTTATCACAGTTG+AGG 0.503180 1_3:-64569581 None:intergenic
TCTTCTACGCATTGCAAGCA+AGG 0.507249 1_3:-64568416 None:intergenic
AAGATAAACGAATCAAACTT+AGG 0.508452 1_3:+64567582 Msa0133300:CDS
CTTGTGTCCGCTGTACTTGG+TGG 0.509443 1_3:-64569430 None:intergenic
ATCAGCGTCGTTAACAGCCA+TGG 0.510244 1_3:-64566960 None:intergenic
TAAGTTTAGGTCTTCAGCTA+TGG 0.514869 1_3:-64569538 None:intergenic
CAGTGACAATGAAAGTGATA+AGG 0.519701 1_3:+64569083 Msa0133300:three_prime_UTR
CTTGATAATTTGACTACCAA+GGG 0.531078 1_3:+64568115 Msa0133300:CDS
TTCAATATAGTGTGCTCCAA+TGG 0.533972 1_3:-64568017 None:intergenic
CACCTTTGTCGATAATTCTG+AGG 0.534885 1_3:-64568307 None:intergenic
CACAAGCATCTTGGGCAGCA+AGG 0.535870 1_3:-64569461 None:intergenic
AGCAAGAAGGTATAGACCCT+TGG 0.540496 1_3:-64568131 None:intergenic
AAATACCTCTCTGGAAAGGA+AGG 0.542272 1_3:-64569872 None:intergenic
GCCGGACTATGAATTTGACC+AGG 0.542943 1_3:+64569023 Msa0133300:three_prime_UTR
CCTTGGTAGTCAAATTATCA+AGG 0.544621 1_3:-64568114 None:intergenic
CAGTAGTAAATAAGAGCTTA+CGG 0.549337 1_3:+64568788 Msa0133300:three_prime_UTR
AAGTGAGAATTGTATCCCTC+AGG 0.551342 1_3:+64568039 Msa0133300:CDS
AGCTGTAACTCTCTTAGCAC+TGG 0.557835 1_3:-64568745 None:intergenic
TTTCATAATATCACTCTGCA+GGG 0.559881 1_3:-64568683 None:intergenic
AACGAATCAAACTTAGGAGG+AGG 0.565962 1_3:+64567588 Msa0133300:CDS
TCATCATCTAAGAAGCGTAA+AGG 0.568425 1_3:+64569812 Msa0133300:three_prime_UTR
TTACCTCTCACCACAAAGGA+GGG 0.569474 1_3:+64567702 Msa0133300:CDS
CCTTGATAATTTGACTACCA+AGG 0.570856 1_3:+64568114 Msa0133300:CDS
GACTTATGATAATAGGACTC+AGG 0.574609 1_3:+64570210 Msa0133300:three_prime_UTR
TTGTGTCCGCTGTACTTGGT+GGG 0.576681 1_3:-64569429 None:intergenic
TAATCTCATAGGTCGTATGT+TGG 0.576899 1_3:+64569506 Msa0133300:three_prime_UTR
AGTTTAGTACACCTATGTCT+AGG 0.577720 1_3:+64569268 Msa0133300:three_prime_UTR
TGAAAGAAATTTGCAAAACC+GGG 0.580059 1_3:+64567733 Msa0133300:CDS
CTTCTTGCTATGATAGTTGG+TGG 0.583896 1_3:+64568145 Msa0133300:CDS
TATACGCATCTGTAAAGCCC+TGG 0.591076 1_3:-64569041 None:intergenic
AACTCTCTTAGCACTGGCAA+TGG 0.591095 1_3:-64568739 None:intergenic
GCTGTAGTGAAGGTGTTGTG+TGG 0.596201 1_3:+64568370 Msa0133300:three_prime_UTR
TAACAAATACCTCTCTGGAA+AGG 0.599447 1_3:-64569876 None:intergenic
CAGATGAAAATATTGTCAAG+AGG 0.600107 1_3:+64569331 Msa0133300:three_prime_UTR
ATTGCAAGCAAGGTTTGGGA+TGG 0.612588 1_3:-64568406 None:intergenic
ACAGCTTCTCATAGAACCAA+AGG 0.613174 1_3:+64568762 Msa0133300:three_prime_UTR
ATCCTCAGAATTATCGACAA+AGG 0.615555 1_3:+64568305 Msa0133300:three_prime_UTR
TCACTATTCAAGCATTCACT+GGG 0.615810 1_3:-64569156 None:intergenic
TTATCATGCTGCTGTAGTGA+AGG 0.615817 1_3:+64568360 Msa0133300:three_prime_UTR
CAATGAAAGTGATAAGGATG+AGG 0.617358 1_3:+64569089 Msa0133300:three_prime_UTR
TGAAATCCATATCATCCTGA+GGG 0.617603 1_3:-64568054 None:intergenic
TTTACCTCTCACCACAAAGG+AGG 0.624083 1_3:+64567701 Msa0133300:CDS
TTATTCCTTCCTTTCCAGAG+AGG 0.626159 1_3:+64569867 Msa0133300:three_prime_UTR
ATGCTTTCAGAACTTGATGG+AGG 0.626472 1_3:+64568538 Msa0133300:three_prime_UTR
CACCAACTATCATAGCAAGA+AGG 0.631132 1_3:-64568144 None:intergenic
ATAAACGAATCAAACTTAGG+AGG 0.631274 1_3:+64567585 Msa0133300:CDS
AAGAAATTTGCAAAACCGGG+TGG 0.633677 1_3:+64567736 Msa0133300:CDS
CCGGACTATGAATTTGACCA+GGG 0.634286 1_3:+64569024 Msa0133300:three_prime_UTR
TACCTCTCACCACAAAGGAG+GGG 0.641692 1_3:+64567703 Msa0133300:CDS
TGAGAAGTCTGCTATTGACA+TGG 0.642126 1_3:-64568590 None:intergenic
TCAATTACTCGAACAATAGG+CGG 0.645112 1_3:-64569839 None:intergenic
TCATCTAAGAAGCGTAAAGG+AGG 0.648277 1_3:+64569815 Msa0133300:three_prime_UTR
CTTTACAGATGCGTATATGG+AGG 0.653691 1_3:+64569047 Msa0133300:three_prime_UTR
TTGAAATCCATATCATCCTG+AGG 0.654948 1_3:-64568055 None:intergenic
AGGTTTACCTCTCACCACAA+AGG 0.659376 1_3:+64567698 Msa0133300:CDS
CCCATACCCACCAAGTACAG+CGG 0.670328 1_3:+64569423 Msa0133300:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTCAAAAAAAAAAAAAAAAA+CGG - chr1_3:64567222-64567241 None:intergenic 10.0%
!! CAATATTTAATTGTATAAAT+TGG - chr1_3:64569658-64569677 None:intergenic 10.0%
!! TATTTAGAAAAATAAAATCA+TGG - chr1_3:64569970-64569989 None:intergenic 10.0%
!! GTTATTATTATGTAATTCAA+TGG + chr1_3:64567013-64567032 Msa0133300:intron 15.0%
!! GGAATATAATAATACAATAA+AGG - chr1_3:64567888-64567907 None:intergenic 15.0%
!! TATTTAATTGTATAAATTGG+TGG - chr1_3:64569655-64569674 None:intergenic 15.0%
!! TACAATTAAATATTGTTGTA+TGG + chr1_3:64569663-64569682 Msa0133300:intron 15.0%
!! AATTAAACTATATTTCCCTT+AGG + chr1_3:64567486-64567505 Msa0133300:intron 20.0%
!! TACATTAATTAAAATGATGC+AGG + chr1_3:64567667-64567686 Msa0133300:intron 20.0%
!! AAGGAAAAAAGAATTCATTA+GGG - chr1_3:64567803-64567822 None:intergenic 20.0%
!! AAAGGAAAAAAGAATTCATT+AGG - chr1_3:64567804-64567823 None:intergenic 20.0%
!!! AATATGAACCTTTTTAAGTT+TGG - chr1_3:64568088-64568107 None:intergenic 20.0%
!!! GATATTAGGTAGTTTTTTTT+AGG + chr1_3:64570086-64570105 Msa0133300:intron 20.0%
!!! GTATGAAATTTTGGAATTAA+TGG - chr1_3:64570352-64570371 None:intergenic 20.0%
! AGGAGTAGTTATAATTAAGA+GGG + chr1_3:64567066-64567085 Msa0133300:intron 25.0%
! ACTTCTTGTGTAAAAAAACA+TGG + chr1_3:64567145-64567164 Msa0133300:intron 25.0%
! AAAAAAACATGGCAAAATTG+TGG + chr1_3:64567156-64567175 Msa0133300:intron 25.0%
! AAAAAACATGGCAAAATTGT+GGG + chr1_3:64567157-64567176 Msa0133300:intron 25.0%
! TTAAATCATTTGTTCCAGAA+TGG - chr1_3:64567387-64567406 None:intergenic 25.0%
!! TAAGTTTGATTCGTTTATCT+TGG - chr1_3:64567583-64567602 None:intergenic 25.0%
! AAGATAAACGAATCAAACTT+AGG + chr1_3:64567582-64567601 Msa0133300:CDS 25.0%
!!! AATCGTCATTTTTGTCTTTT+TGG + chr1_3:64567612-64567631 Msa0133300:intron 25.0%
!!! ATCGTCATTTTTGTCTTTTT+GGG + chr1_3:64567613-64567632 Msa0133300:intron 25.0%
! ATTAATTAAAATGATGCAGG+TGG + chr1_3:64567670-64567689 Msa0133300:intron 25.0%
! TTGAAAGAAATTTGCAAAAC+CGG + chr1_3:64567732-64567751 Msa0133300:CDS 25.0%
!! TGTTAAATGATTTAGTCTTC+AGG + chr1_3:64567772-64567791 Msa0133300:intron 25.0%
! TGTAGACAAGAAAAGAAAAA+AGG - chr1_3:64567822-64567841 None:intergenic 25.0%
!!! TTTTTTCTTTTCTTGTCTAC+AGG + chr1_3:64567821-64567840 Msa0133300:intron 25.0%
! ACCAAATACCAAACTTAAAA+AGG + chr1_3:64568077-64568096 Msa0133300:CDS 25.0%
!!! ACCTTTTTAAGTTTGGTATT+TGG - chr1_3:64568081-64568100 None:intergenic 25.0%
! TTCTCACTAAAACTGATAAT+TGG + chr1_3:64568607-64568626 Msa0133300:three_prime_UTR 25.0%
!!! CTAAAACTGATAATTGGTTT+TGG + chr1_3:64568613-64568632 Msa0133300:three_prime_UTR 25.0%
! TATATTGTCTGTTGAGAAAA+AGG + chr1_3:64568708-64568727 Msa0133300:three_prime_UTR 25.0%
! GAACTGTGATACAATAAATT+TGG + chr1_3:64569188-64569207 Msa0133300:three_prime_UTR 25.0%
! TTTGTTACATTCCTCAATTA+TGG + chr1_3:64569892-64569911 Msa0133300:intron 25.0%
! AAAGATGCAAATGACTTAAA+GGG - chr1_3:64570038-64570057 None:intergenic 25.0%
! CATACTCAGATATAAAAACA+TGG - chr1_3:64570135-64570154 None:intergenic 25.0%
!!! ATTGATGGACTTATGATAAT+AGG + chr1_3:64570203-64570222 Msa0133300:three_prime_UTR 25.0%
!!! TTGAAATGGTTTTGCATTTT+TGG + chr1_3:64570261-64570280 Msa0133300:three_prime_UTR 25.0%
! CAGTGTAAAAACTCATAATT+TGG - chr1_3:64570297-64570316 None:intergenic 25.0%
!! AATTCTCTTCTGTTTTATAG+TGG + chr1_3:64570382-64570401 Msa0133300:three_prime_UTR 25.0%
! ACAAAGCTTCAATTAATTTC+AGG + chr1_3:64570405-64570424 Msa0133300:three_prime_UTR 25.0%
GAGGAGTAGTTATAATTAAG+AGG + chr1_3:64567065-64567084 Msa0133300:intron 30.0%
GGAGTAGTTATAATTAAGAG+GGG + chr1_3:64567067-64567086 Msa0133300:intron 30.0%
! TTTACACAAGAAGTTGTTTC+CGG - chr1_3:64567139-64567158 None:intergenic 30.0%
AAAAACATGGCAAAATTGTG+GGG + chr1_3:64567158-64567177 Msa0133300:intron 30.0%
ACAAAAGCAAAAGCAAAAAC+AGG - chr1_3:64567438-64567457 None:intergenic 30.0%
AAACTATATTTCCCTTAGGT+TGG + chr1_3:64567490-64567509 Msa0133300:intron 30.0%
ATAAACGAATCAAACTTAGG+AGG + chr1_3:64567585-64567604 Msa0133300:CDS 30.0%
!!! ATTTTTGTCTTTTTGGGTGT+TGG + chr1_3:64567619-64567638 Msa0133300:intron 30.0%
TGAAAGAAATTTGCAAAACC+GGG + chr1_3:64567733-64567752 Msa0133300:CDS 30.0%
ATCTTGTCTGAGTAATGATT+CGG - chr1_3:64567854-64567873 None:intergenic 30.0%
! CTTGATAATTTGACTACCAA+GGG + chr1_3:64568115-64568134 Msa0133300:CDS 30.0%
TAAAATTGTGACTCAGATCT+AGG - chr1_3:64568343-64568362 None:intergenic 30.0%
TATTCACTTGTCTGATTAGA+TGG - chr1_3:64568504-64568523 None:intergenic 30.0%
TTTCATAATATCACTCTGCA+GGG - chr1_3:64568686-64568705 None:intergenic 30.0%
TTATTTACTACTGTAGCCTT+TGG - chr1_3:64568781-64568800 None:intergenic 30.0%
CAGTAGTAAATAAGAGCTTA+CGG + chr1_3:64568788-64568807 Msa0133300:three_prime_UTR 30.0%
! TACATCATGCATGCTTTTAT+TGG - chr1_3:64568917-64568936 None:intergenic 30.0%
!! AATTATTCAAGACTTGTTGC+CGG + chr1_3:64569005-64569024 Msa0133300:three_prime_UTR 30.0%
!! TCTTGGCATTGGTTATTATA+TGG - chr1_3:64569234-64569253 None:intergenic 30.0%
! CTCTTGACAATATTTTCATC+TGG - chr1_3:64569333-64569352 None:intergenic 30.0%
CAGATGAAAATATTGTCAAG+AGG + chr1_3:64569331-64569350 Msa0133300:three_prime_UTR 30.0%
ATGCTTGCATATAATCTCAT+AGG + chr1_3:64569495-64569514 Msa0133300:three_prime_UTR 30.0%
TCTTACTCAAGTCTAAGTTT+AGG - chr1_3:64569554-64569573 None:intergenic 30.0%
TAAACTTAGACTTGAGTAAG+AGG + chr1_3:64569553-64569572 Msa0133300:three_prime_UTR 30.0%
AAGCAAAGTGAAGACATAAA+AGG + chr1_3:64569594-64569613 Msa0133300:three_prime_UTR 30.0%
! ATTGTATAAATTGGTGGTAG+CGG - chr1_3:64569649-64569668 None:intergenic 30.0%
ATATTGTTGTATGGTCATAC+TGG + chr1_3:64569672-64569691 Msa0133300:intron 30.0%
! ATGACAGTTAAGTAGTGTTT+TGG + chr1_3:64569716-64569735 Msa0133300:intron 30.0%
CTTTACTCATTTGCATATGT+GGG + chr1_3:64569992-64570011 Msa0133300:intron 30.0%
CAAAGATGCAAATGACTTAA+AGG - chr1_3:64570039-64570058 None:intergenic 30.0%
AGATGCGTAACAAAGATATT+AGG + chr1_3:64570072-64570091 Msa0133300:intron 30.0%
!! CTCTAAGGAGTATGAAATTT+TGG - chr1_3:64570361-64570380 None:intergenic 30.0%
TATAATTAAGAGGGGAGCTT+TGG + chr1_3:64567075-64567094 Msa0133300:intron 35.0%
AAAACATGGCAAAATTGTGG+GGG + chr1_3:64567159-64567178 Msa0133300:intron 35.0%
TGCACAAGTGAATTAGATAC+AGG - chr1_3:64567293-64567312 None:intergenic 35.0%
TCTAATTCACTTGTGCATCA+TGG + chr1_3:64567296-64567315 Msa0133300:intron 35.0%
CTAATTCACTTGTGCATCAT+GGG + chr1_3:64567297-64567316 Msa0133300:intron 35.0%
!!! ACCTCTTTTTTGTTCCATTC+TGG + chr1_3:64567370-64567389 Msa0133300:intron 35.0%
TCCAGAATGGAACAAAAAAG+AGG - chr1_3:64567374-64567393 None:intergenic 35.0%
AAATGATGCAGGTGGTTATT+AGG + chr1_3:64567678-64567697 Msa0133300:intron 35.0%
TCTTGTCTGAGTAATGATTC+GGG - chr1_3:64567853-64567872 None:intergenic 35.0%
TTACTCAGACAAGATGATGT+AGG + chr1_3:64567859-64567878 Msa0133300:intron 35.0%
AGAACAAAGCAAGTAACTCT+AGG - chr1_3:64567909-64567928 None:intergenic 35.0%
TTCAATATAGTGTGCTCCAA+TGG - chr1_3:64568020-64568039 None:intergenic 35.0%
TGAAATCCATATCATCCTGA+GGG - chr1_3:64568057-64568076 None:intergenic 35.0%
TTGAAATCCATATCATCCTG+AGG - chr1_3:64568058-64568077 None:intergenic 35.0%
! CCTTGGTAGTCAAATTATCA+AGG - chr1_3:64568117-64568136 None:intergenic 35.0%
! CCTTGATAATTTGACTACCA+AGG + chr1_3:64568114-64568133 Msa0133300:CDS 35.0%
! TACCTTCTTGCTATGATAGT+TGG + chr1_3:64568142-64568161 Msa0133300:CDS 35.0%
AAGAAACAAAGACCAACAAC+TGG + chr1_3:64568249-64568268 Msa0133300:CDS 35.0%
ATCCTCAGAATTATCGACAA+AGG + chr1_3:64568305-64568324 Msa0133300:three_prime_UTR 35.0%
AAGATGCTTTCAGAACTTGA+TGG + chr1_3:64568535-64568554 Msa0133300:three_prime_UTR 35.0%
CTTTCATAATATCACTCTGC+AGG - chr1_3:64568687-64568706 None:intergenic 35.0%
AGAATCTGCAATAGAGACTT+TGG - chr1_3:64568874-64568893 None:intergenic 35.0%
CTTCTTCAATATGCTTCTTC+TGG - chr1_3:64568945-64568964 None:intergenic 35.0%
CAGTGACAATGAAAGTGATA+AGG + chr1_3:64569083-64569102 Msa0133300:three_prime_UTR 35.0%
CAATGAAAGTGATAAGGATG+AGG + chr1_3:64569089-64569108 Msa0133300:three_prime_UTR 35.0%
TCACTATTCAAGCATTCACT+GGG - chr1_3:64569159-64569178 None:intergenic 35.0%
ATCACTATTCAAGCATTCAC+TGG - chr1_3:64569160-64569179 None:intergenic 35.0%
AGTTTAGTACACCTATGTCT+AGG + chr1_3:64569268-64569287 Msa0133300:three_prime_UTR 35.0%
! TCACAGTTTTTCCTAGACAT+AGG - chr1_3:64569282-64569301 None:intergenic 35.0%
CAAAAGTTGGATTCATCTCT+TGG - chr1_3:64569390-64569409 None:intergenic 35.0%
!!! TTTTTTCTCCTCGCAAAAGT+TGG - chr1_3:64569403-64569422 None:intergenic 35.0%
TGTCTTATCACAAGCATCTT+GGG - chr1_3:64569472-64569491 None:intergenic 35.0%
ATGTCTTATCACAAGCATCT+TGG - chr1_3:64569473-64569492 None:intergenic 35.0%
TAATCTCATAGGTCGTATGT+TGG + chr1_3:64569506-64569525 Msa0133300:three_prime_UTR 35.0%
TAAGTTTAGGTCTTCAGCTA+TGG - chr1_3:64569541-64569560 None:intergenic 35.0%
! TCATCATCTAAGAAGCGTAA+AGG + chr1_3:64569812-64569831 Msa0133300:three_prime_UTR 35.0%
TCAATTACTCGAACAATAGG+CGG - chr1_3:64569842-64569861 None:intergenic 35.0%
TCCTCAATTACTCGAACAAT+AGG - chr1_3:64569845-64569864 None:intergenic 35.0%
! TAACAAATACCTCTCTGGAA+AGG - chr1_3:64569879-64569898 None:intergenic 35.0%
GAATGTAACAAATACCTCTC+TGG - chr1_3:64569884-64569903 None:intergenic 35.0%
TTCGTATGCTACCATAATTG+AGG - chr1_3:64569906-64569925 None:intergenic 35.0%
GCTTTACTCATTTGCATATG+TGG + chr1_3:64569991-64570010 Msa0133300:intron 35.0%
GGAAAGACTGAAGATATTGA+TGG + chr1_3:64570188-64570207 Msa0133300:three_prime_UTR 35.0%
GACTTATGATAATAGGACTC+AGG + chr1_3:64570210-64570229 Msa0133300:three_prime_UTR 35.0%
AACAGAAGAGAATTGCTCTA+AGG - chr1_3:64570376-64570395 None:intergenic 35.0%
TAGAGATGTGTTCGATTACG+AGG + chr1_3:64567046-64567065 Msa0133300:intron 40.0%
!!! TCACGTGACTTTTCAAGTTC+CGG + chr1_3:64567117-64567136 Msa0133300:intron 40.0%
AAACATGGCAAAATTGTGGG+GGG + chr1_3:64567160-64567179 Msa0133300:intron 40.0%
ACTAAGCTGATCCAACCTAA+GGG - chr1_3:64567504-64567523 None:intergenic 40.0%
AACTAAGCTGATCCAACCTA+AGG - chr1_3:64567505-64567524 None:intergenic 40.0%
AACGAATCAAACTTAGGAGG+AGG + chr1_3:64567588-64567607 Msa0133300:CDS 40.0%
AAGAAATTTGCAAAACCGGG+TGG + chr1_3:64567736-64567755 Msa0133300:CDS 40.0%
AAGTGAGAATTGTATCCCTC+AGG + chr1_3:64568039-64568058 Msa0133300:CDS 40.0%
TTGTATCCCTCAGGATGATA+TGG + chr1_3:64568048-64568067 Msa0133300:CDS 40.0%
CACCAACTATCATAGCAAGA+AGG - chr1_3:64568147-64568166 None:intergenic 40.0%
! CTTCTTGCTATGATAGTTGG+TGG + chr1_3:64568145-64568164 Msa0133300:CDS 40.0%
CACCTTTGTCGATAATTCTG+AGG - chr1_3:64568310-64568329 None:intergenic 40.0%
TTATCATGCTGCTGTAGTGA+AGG + chr1_3:64568360-64568379 Msa0133300:three_prime_UTR 40.0%
GCAATGCGTAGAAGACTTAA+AGG + chr1_3:64568424-64568443 Msa0133300:three_prime_UTR 40.0%
ATGCTTTCAGAACTTGATGG+AGG + chr1_3:64568538-64568557 Msa0133300:three_prime_UTR 40.0%
!! ATTGACATGGCTTTGGCTAA+TGG - chr1_3:64568580-64568599 None:intergenic 40.0%
TGAGAAGTCTGCTATTGACA+TGG - chr1_3:64568593-64568612 None:intergenic 40.0%
ACAGCTTCTCATAGAACCAA+AGG + chr1_3:64568762-64568781 Msa0133300:three_prime_UTR 40.0%
TCTCTTTGAGTGCAGTGATA+TGG + chr1_3:64568840-64568859 Msa0133300:three_prime_UTR 40.0%
CTTTACAGATGCGTATATGG+AGG + chr1_3:64569047-64569066 Msa0133300:three_prime_UTR 40.0%
CTGTGATTTCTTCTTGGCAT+TGG - chr1_3:64569245-64569264 None:intergenic 40.0%
ACTGCTCTGTGATTTCTTCT+TGG - chr1_3:64569251-64569270 None:intergenic 40.0%
! AGATGAATCCAACTTTTGCG+AGG + chr1_3:64569392-64569411 Msa0133300:three_prime_UTR 40.0%
CACTTTGCTTATCACAGTTG+AGG - chr1_3:64569584-64569603 None:intergenic 40.0%
! TCATCTAAGAAGCGTAAAGG+AGG + chr1_3:64569815-64569834 Msa0133300:three_prime_UTR 40.0%
GCCTATTGTTCGAGTAATTG+AGG + chr1_3:64569841-64569860 Msa0133300:three_prime_UTR 40.0%
TTATTCCTTCCTTTCCAGAG+AGG + chr1_3:64569867-64569886 Msa0133300:three_prime_UTR 40.0%
! AAATACCTCTCTGGAAAGGA+AGG - chr1_3:64569875-64569894 None:intergenic 40.0%
AAATGACTTAAAGGGCAACC+CGG - chr1_3:64570030-64570049 None:intergenic 40.0%
! GTATGCGTAACTCTTGCTTT+TGG + chr1_3:64570150-64570169 Msa0133300:intron 40.0%
ATAATAGGACTCAGGCTTTC+AGG + chr1_3:64570218-64570237 Msa0133300:three_prime_UTR 40.0%
CATTTCAAAATGCTCTTCGC+TGG - chr1_3:64570249-64570268 None:intergenic 40.0%
!!! CAGCGAAGAGCATTTTGAAA+TGG + chr1_3:64570247-64570266 Msa0133300:three_prime_UTR 40.0%
TAGTTCCAGCTTCTCAGCTT+CGG + chr1_3:64567258-64567277 Msa0133300:intron 45.0%
TATTTCCGAAGCTGAGAAGC+TGG - chr1_3:64567266-64567285 None:intergenic 45.0%
AGGTTTACCTCTCACCACAA+AGG + chr1_3:64567698-64567717 Msa0133300:CDS 45.0%
TTTACCTCTCACCACAAAGG+AGG + chr1_3:64567701-64567720 Msa0133300:CDS 45.0%
TTACCTCTCACCACAAAGGA+GGG + chr1_3:64567702-64567721 Msa0133300:CDS 45.0%
! AGCAAGAAGGTATAGACCCT+TGG - chr1_3:64568134-64568153 None:intergenic 45.0%
GTTTGCGAGTTTCCAGTTGT+TGG - chr1_3:64568264-64568283 None:intergenic 45.0%
ATTGCAAGCAAGGTTTGGGA+TGG - chr1_3:64568409-64568428 None:intergenic 45.0%
ACGCATTGCAAGCAAGGTTT+GGG - chr1_3:64568413-64568432 None:intergenic 45.0%
TACGCATTGCAAGCAAGGTT+TGG - chr1_3:64568414-64568433 None:intergenic 45.0%
TCTTCTACGCATTGCAAGCA+AGG - chr1_3:64568419-64568438 None:intergenic 45.0%
! TTTTTGCAAGGTTGCAGCTG+AGG - chr1_3:64568466-64568485 None:intergenic 45.0%
CAGCTGCAACCTTGCAAAAA+CGG + chr1_3:64568466-64568485 Msa0133300:three_prime_UTR 45.0%
TGCAACCTTGCAAAAACGGA+TGG + chr1_3:64568470-64568489 Msa0133300:three_prime_UTR 45.0%
GCAACCTTGCAAAAACGGAT+GGG + chr1_3:64568471-64568490 Msa0133300:three_prime_UTR 45.0%
CAACCTTGCAAAAACGGATG+GGG + chr1_3:64568472-64568491 Msa0133300:three_prime_UTR 45.0%
GTCTGCTATTGACATGGCTT+TGG - chr1_3:64568587-64568606 None:intergenic 45.0%
!! AACTCTCTTAGCACTGGCAA+TGG - chr1_3:64568742-64568761 None:intergenic 45.0%
AGCTGTAACTCTCTTAGCAC+TGG - chr1_3:64568748-64568767 None:intergenic 45.0%
CCCTGGTCAAATTCATAGTC+CGG - chr1_3:64569027-64569046 None:intergenic 45.0%
CCGGACTATGAATTTGACCA+GGG + chr1_3:64569024-64569043 Msa0133300:three_prime_UTR 45.0%
TATACGCATCTGTAAAGCCC+TGG - chr1_3:64569044-64569063 None:intergenic 45.0%
GGGCTTTACAGATGCGTATA+TGG + chr1_3:64569044-64569063 Msa0133300:three_prime_UTR 45.0%
ATAAAGCTCCCCCATACGCA+TGG - chr1_3:64567195-64567214 None:intergenic 50.0%
TACCTCTCACCACAAAGGAG+GGG + chr1_3:64567703-64567722 Msa0133300:CDS 50.0%
CAACTTCTTCCCCTCCTTTG+TGG - chr1_3:64567715-64567734 None:intergenic 50.0%
ACTCATGTTGAACGCGCCAT+TGG + chr1_3:64568001-64568020 Msa0133300:CDS 50.0%
!! GCTGTAGTGAAGGTGTTGTG+TGG + chr1_3:64568370-64568389 Msa0133300:three_prime_UTR 50.0%
CTCTGCAGGGATTTCACTTC+AGG - chr1_3:64568673-64568692 None:intergenic 50.0%
GCCGGACTATGAATTTGACC+AGG + chr1_3:64569023-64569042 Msa0133300:three_prime_UTR 50.0%
TTGTGTCCGCTGTACTTGGT+GGG - chr1_3:64569432-64569451 None:intergenic 50.0%
GTTCTTGTGTCCGCTGTACT+TGG - chr1_3:64569436-64569455 None:intergenic 50.0%
CATCTTGGGCAGCAAGGTAT+TGG - chr1_3:64569458-64569477 None:intergenic 50.0%
! GGTAGCGGTCATAGCAGTAA+CGG - chr1_3:64569634-64569653 None:intergenic 50.0%
!! CTTGCTTTTGGACACTGCAG+TGG + chr1_3:64570162-64570181 Msa0133300:intron 50.0%
! TTTTGGACACTGCAGTGGCA+TGG + chr1_3:64570167-64570186 Msa0133300:intron 50.0%
! TAAGAGGGGAGCTTTGGTGC+AGG + chr1_3:64567081-64567100 Msa0133300:intron 55.0%
! AAGAGGGGAGCTTTGGTGCA+GGG + chr1_3:64567082-64567101 Msa0133300:intron 55.0%
CAAAATTGTGGGGGGCTTCC+CGG + chr1_3:64567168-64567187 Msa0133300:intron 55.0%
TTCCCCTCCTTTGTGGTGAG+AGG - chr1_3:64567708-64567727 None:intergenic 55.0%
! CAGATGAAGCACTCACCACC+CGG - chr1_3:64567754-64567773 None:intergenic 55.0%
CAAGGTTGCAGCTGAGGCAT+CGG - chr1_3:64568460-64568479 None:intergenic 55.0%
!! CGTCCCCATCCGTTTTTGCA+AGG - chr1_3:64568478-64568497 None:intergenic 55.0%
! CCGCTGTACTTGGTGGGTAT+GGG - chr1_3:64569426-64569445 None:intergenic 55.0%
CCCATACCCACCAAGTACAG+CGG + chr1_3:64569423-64569442 Msa0133300:three_prime_UTR 55.0%
! TCCGCTGTACTTGGTGGGTA+TGG - chr1_3:64569427-64569446 None:intergenic 55.0%
CTTGTGTCCGCTGTACTTGG+TGG - chr1_3:64569433-64569452 None:intergenic 55.0%
CACAAGCATCTTGGGCAGCA+AGG - chr1_3:64569464-64569483 None:intergenic 55.0%
! ATGTGGGAGCTCTAGTGCAC+CGG + chr1_3:64570008-64570027 Msa0133300:intron 55.0%
GCTTCCCGGACCATGCGTAT+GGG + chr1_3:64567182-64567201 Msa0133300:intron 60.0%
CTTCCCGGACCATGCGTATG+GGG + chr1_3:64567183-64567202 Msa0133300:intron 60.0%
TTCCCGGACCATGCGTATGG+GGG + chr1_3:64567184-64567203 Msa0133300:intron 60.0%
! TGTGGGAGCTCTAGTGCACC+GGG + chr1_3:64570009-64570028 Msa0133300:intron 60.0%
GGCTTCCCGGACCATGCGTA+TGG + chr1_3:64567181-64567200 Msa0133300:intron 65.0%
CTCCCCCATACGCATGGTCC+GGG - chr1_3:64567189-64567208 None:intergenic 65.0%
GCTCCCCCATACGCATGGTC+CGG - chr1_3:64567190-64567209 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 64566962 64570449 64566962 ID=Msa0133300;Name=Msa0133300
chr1_3 mRNA 64566962 64570449 64566962 ID=Msa0133300-mRNA-1;Parent=Msa0133300;Name=Msa0133300-mRNA-1;_AED=0.03;_eAED=0.03;_QI=0|0.83|0.71|0.85|1|0.85|7|1677|181
chr1_3 exon 64566962 64567023 64566962 ID=Msa0133300-mRNA-1:exon:21698;Parent=Msa0133300-mRNA-1
chr1_3 exon 64567544 64567609 64567544 ID=Msa0133300-mRNA-1:exon:21699;Parent=Msa0133300-mRNA-1
chr1_3 exon 64567689 64567757 64567689 ID=Msa0133300-mRNA-1:exon:21700;Parent=Msa0133300-mRNA-1
chr1_3 exon 64567843 64567876 64567843 ID=Msa0133300-mRNA-1:exon:21701;Parent=Msa0133300-mRNA-1
chr1_3 exon 64567980 64569615 64567980 ID=Msa0133300-mRNA-1:exon:21702;Parent=Msa0133300-mRNA-1
chr1_3 exon 64569801 64569888 64569801 ID=Msa0133300-mRNA-1:exon:21703;Parent=Msa0133300-mRNA-1
chr1_3 exon 64570182 64570449 64570182 ID=Msa0133300-mRNA-1:exon:21704;Parent=Msa0133300-mRNA-1
chr1_3 CDS 64566962 64567023 64566962 ID=Msa0133300-mRNA-1:cds;Parent=Msa0133300-mRNA-1
chr1_3 CDS 64567544 64567609 64567544 ID=Msa0133300-mRNA-1:cds;Parent=Msa0133300-mRNA-1
chr1_3 CDS 64567689 64567757 64567689 ID=Msa0133300-mRNA-1:cds;Parent=Msa0133300-mRNA-1
chr1_3 CDS 64567843 64567876 64567843 ID=Msa0133300-mRNA-1:cds;Parent=Msa0133300-mRNA-1
chr1_3 CDS 64567980 64568294 64567980 ID=Msa0133300-mRNA-1:cds;Parent=Msa0133300-mRNA-1
chr1_3 three_prime_UTR 64568295 64569615 64568295 ID=Msa0133300-mRNA-1:three_prime_utr;Parent=Msa0133300-mRNA-1
chr1_3 three_prime_UTR 64569801 64569888 64569801 ID=Msa0133300-mRNA-1:three_prime_utr;Parent=Msa0133300-mRNA-1
chr1_3 three_prime_UTR 64570182 64570449 64570182 ID=Msa0133300-mRNA-1:three_prime_utr;Parent=Msa0133300-mRNA-1
Gene Sequence

>Msa0133300

ATGGCTGTTAACGACGCTGATCAATCTCACAAAGCTCACAGTACTGTTACTGTTATTATTATAACAGATATCTGTTCTATTTTAAGAGATGCAAAGCGCCAAGATAAACGAATCAAACTTAGGAGGAGGTGGTTATTAGGTTTACCTCTCACCACAAAGGAGGGGAAGAAGTTGAAAGAAATTTGCAAAACCGGGTGGTATTCACCCGAATCATTACTCAGACAAGATGATCTATTTTATGAGTCAGTGAGAACTCATGTTGAACGCGCCATTGGAGCACACTATATTGAAAGTGAGAATTGTATCCCTCAGGATGATATGGATTTCAACCAAATACCAAACTTAAAAAGGTTCATATTATCATGCCTTGATAATTTGACTACCAAGGGTCTATACCTTCTTGCTATGATAGTTGGTGGTGACACAATCAAGTATGAAAGAACTCGAAGTAATTTGAAAAAGATTATCAAGAGTTCTCTTTCAAGCGTTCTGAGTAGCGAAAGAAACAAAGACCAACAACTGGAAACTCGCAAACAGCTTTTTTAA
Protein sequence

>Msa0133300

MAVNDADQSHKAHSTVTVIIITDICSILRDAKRQDKRIKLRRRWLLGLPLTTKEGKKLKEICKTGWYSPESLLRQDDLFYESVRTHVERAIGAHYIESENCIPQDDMDFNQIPNLKRFILSCLDNLTTKGLYLLAMIVGGDTIKYERTRSNLKKIIKSSLSSVLSSERNKDQQLETRKQLF*