AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0133510

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0133510 MtrunA17_Chr1g0203001 94.967 457 22 1 1 457 1 456 0.0 854
Msa0133510 MtrunA17_Chr8g0381341 35.258 329 197 4 103 427 313 629 3.52e-35 138
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0133510 AT3G49055.1 49.191 309 145 4 125 432 156 453 4.13e-72 236
Msa0133510 AT1G24560.1 36.826 334 194 7 125 453 351 672 4.54e-34 135

Find 88 sgRNAs with CRISPR-Local

Find 170 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAAGAAAGGATTGCATTTC+TGG 0.079330 1_3:-64785161 None:intergenic
TCAGAGGTGTAGGGTGAATT+TGG 0.200366 1_3:-64782403 None:intergenic
CATTTCTGGTATCATCCTTT+TGG 0.244152 1_3:-64785147 None:intergenic
CACTCAATTGAGAAGATCTT+TGG 0.250215 1_3:+64783845 Msa0133510:CDS
TGAATCCTTCATTTCAATAA+AGG 0.255904 1_3:+64782576 Msa0133510:CDS
TAAAGGGACATGATCATAAA+AGG 0.299279 1_3:+64782899 Msa0133510:CDS
TTATAAGGAGTTAAGGAAAA+AGG 0.301417 1_3:+64782768 Msa0133510:CDS
CTTGCTTAGGGTTGCTTTGT+TGG 0.306411 1_3:+64782852 Msa0133510:CDS
GGGATATTAATAACTTGCTT+AGG 0.323686 1_3:+64782839 Msa0133510:CDS
AACAAAGGTGGAAAATTATA+AGG 0.327278 1_3:+64782753 Msa0133510:CDS
TGAATCAACGGTGTTGTTAT+TGG 0.327284 1_3:+64782690 Msa0133510:CDS
CTCTGATTCCTCTAGTTGTC+TGG 0.328896 1_3:-64785018 None:intergenic
CAAGTTTAACTGAAGAGAAT+AGG 0.334464 1_3:+64782818 Msa0133510:CDS
GGCATTGGAGAAGAAAATAA+AGG 0.348101 1_3:+64782882 Msa0133510:CDS
CTCAAAACAGTTTCAAAGTA+AGG 0.362902 1_3:-64782460 None:intergenic
GATTGAAGAGCGTGACAAAA+TGG 0.368630 1_3:+64784496 Msa0133510:CDS
TTCTCAATTGAGTGATTTCA+AGG 0.377950 1_3:-64783836 None:intergenic
AATATTGCTACCTCTAGAAT+TGG 0.380419 1_3:+64785119 Msa0133510:CDS
TGAAGTGGAAGTTGTTAGTT+TGG 0.382314 1_3:+64783050 Msa0133510:CDS
GGAATCCTTTATTGAAATGA+AGG 0.382840 1_3:-64782581 None:intergenic
TGGAAAATTATAAGGAGTTA+AGG 0.385060 1_3:+64782761 Msa0133510:CDS
AGAGAGTTTAGAGGGAGAAA+AGG 0.399871 1_3:+64782618 Msa0133510:CDS
GCAGAAAACAAAGGGTGCTT+TGG 0.404761 1_3:+64784860 Msa0133510:CDS
GAAGATCTTTGGAAGAATCT+AGG 0.411043 1_3:+64783856 Msa0133510:CDS
CAGAAGAAGAGGTAGCTAGA+TGG 0.415692 1_3:+64784432 Msa0133510:CDS
CTTTATCTGAATGAAGACTA+TGG 0.417754 1_3:-64782435 None:intergenic
ATGGAAGGAAGCTTGTGAAT+TGG 0.425674 1_3:+64784451 Msa0133510:CDS
TAGAGTAAAAGAGAGTTTAG+AGG 0.428291 1_3:+64782609 Msa0133510:CDS
ATTGTTGAGTTTGTACTGAT+TGG 0.430495 1_3:-64782380 None:intergenic
GGTTGCTTTGTTGGAGAAAG+AGG 0.435951 1_3:+64782861 Msa0133510:CDS
AGCTTGTGAATTGGAAGTTA+AGG 0.438841 1_3:+64784460 Msa0133510:CDS
AGTTTGGATTGCAGAAAGTT+GGG 0.445362 1_3:+64782944 Msa0133510:CDS
AGTTTGTACTGATTGGAAGA+TGG 0.446181 1_3:-64782373 None:intergenic
GGATTGCAGAAAGTTGGGTT+TGG 0.447142 1_3:+64782949 Msa0133510:CDS
AACGGTGTTGTTATTGGAAG+AGG 0.451228 1_3:+64782696 Msa0133510:CDS
AACTCAGCAAACTGCAAAAG+TGG 0.459010 1_3:-64782925 None:intergenic
GGATATTAATAACTTGCTTA+GGG 0.460080 1_3:+64782840 Msa0133510:CDS
AAGTTTAACTGAAGAGAATA+GGG 0.463002 1_3:+64782819 Msa0133510:CDS
ATACACATTTCATTGAAGAA+AGG 0.468708 1_3:-64785174 None:intergenic
TTTGTTTCTCTGTGAGACAC+TGG 0.481946 1_3:-64784212 None:intergenic
CTGATTGGAAGATGGGTGAT+AGG 0.487601 1_3:-64782365 None:intergenic
AAGAGGCAAGGTTAATATCA+AGG 0.488668 1_3:+64782713 Msa0133510:CDS
GAGTTTGGATTGCAGAAAGT+TGG 0.488938 1_3:+64782943 Msa0133510:CDS
AGAAGATAGAGAGAGAATCT+TGG 0.496215 1_3:+64784275 Msa0133510:CDS
TGAACTTCGTCGGCAGATAG+AGG 0.497803 1_3:+64784989 Msa0133510:CDS
CATTATCCTTTCCACTGTTG+AGG 0.498340 1_3:-64783802 None:intergenic
TTTGGATTCATGATGGGAGG+TGG 0.499288 1_3:+64782967 Msa0133510:CDS
AGTTGGGTTTGGATTCATGA+TGG 0.502092 1_3:+64782960 Msa0133510:CDS
GTTTGTACTGATTGGAAGAT+GGG 0.507092 1_3:-64782372 None:intergenic
TGGTAATAATGTGCAATCAA+TGG 0.508772 1_3:+64782987 Msa0133510:CDS
GCATTGGAGAAGAAAATAAA+GGG 0.508904 1_3:+64782883 Msa0133510:CDS
AGAGTAAAAGAGAGTTTAGA+GGG 0.509789 1_3:+64782610 Msa0133510:CDS
AATGGCTGCAATAGCTGCAC+AGG 0.512276 1_3:+64784923 Msa0133510:CDS
TGTGAATTGGAAGTTAAGGC+TGG 0.515864 1_3:+64784464 Msa0133510:CDS
AATCAAAGAAACAGAAGAAG+AGG 0.521307 1_3:+64784421 Msa0133510:CDS
CTAGAATTGGAAATGCCAAA+AGG 0.523851 1_3:+64785132 Msa0133510:CDS
GGGTTTGGATTCATGATGGG+AGG 0.525505 1_3:+64782964 Msa0133510:CDS
GTTGGGTTTGGATTCATGAT+GGG 0.528280 1_3:+64782961 Msa0133510:CDS
TTTGTTGGAGAAAGAGGCAT+TGG 0.529077 1_3:+64782867 Msa0133510:CDS
GTCAGAAACTTCAGAGGTGT+AGG 0.536212 1_3:-64782413 None:intergenic
CAAGAACTGCAGAAAACAAA+GGG 0.555928 1_3:+64784852 Msa0133510:CDS
AGATAGCAGTGAGTGTGAAG+TGG 0.556533 1_3:+64783035 Msa0133510:CDS
AATATCAAGGCTTGCAACTG+AGG 0.558159 1_3:+64782726 Msa0133510:CDS
ATCAAGGCTTGCAACTGAGG+TGG 0.576214 1_3:+64782729 Msa0133510:CDS
AGAAGAGGTAGCTAGATGGA+AGG 0.576787 1_3:+64784436 Msa0133510:CDS
TGTCAGAGAAATAGTTTGTG+AGG 0.577043 1_3:+64782651 Msa0133510:CDS
TGTTGTTATTGGAAGAGGCA+AGG 0.579247 1_3:+64782701 Msa0133510:CDS
AGAAAACAAACTCTACATCA+AGG 0.582148 1_3:+64784248 Msa0133510:CDS
CCAAGGCCTCAACAGTGGAA+AGG 0.591423 1_3:+64783796 Msa0133510:intron
GTAGAGGAGTTGAACTTCGT+CGG 0.592524 1_3:+64784979 Msa0133510:CDS
TGCAACTGAGGTGGAAACAA+AGG 0.599427 1_3:+64782738 Msa0133510:CDS
TTTGGCATTTCCAATTCTAG+AGG 0.603440 1_3:-64785129 None:intergenic
GGAACTAACCAGACAACTAG+AGG 0.607450 1_3:+64785010 Msa0133510:CDS
TGTATTGGATGGAAATGAAG+TGG 0.608267 1_3:+64782495 Msa0133510:CDS
GTTTCTCTGTGAGACACTGG+AGG 0.609222 1_3:-64784209 None:intergenic
TCAGAAACTTCAGAGGTGTA+GGG 0.615156 1_3:-64782412 None:intergenic
AGAGTTAAGTGATGAATCAA+CGG 0.616622 1_3:+64782678 Msa0133510:CDS
GGTGCTTTGGACATATCCAA+TGG 0.618935 1_3:+64784873 Msa0133510:CDS
ATCAATGGATGGTACTTCTG+AGG 0.620756 1_3:+64783002 Msa0133510:CDS
AAGTGAGTCGACGTATATGT+TGG 0.622198 1_3:+64785066 Msa0133510:CDS
AATAATGTGCAATCAATGGA+TGG 0.631660 1_3:+64782991 Msa0133510:CDS
ACAAGAACTGCAGAAAACAA+AGG 0.632471 1_3:+64784851 Msa0133510:CDS
TTGAAGCTAGCTGATAGTAG+AGG 0.633294 1_3:+64784963 Msa0133510:CDS
GTCGACGTATATGTTGGCCA+TGG 0.640311 1_3:+64785072 Msa0133510:CDS
ACTCAAAGAAGAGCTAGCAA+TGG 0.653656 1_3:+64784905 Msa0133510:CDS
AACTGAGGTGGAAACAAAGG+TGG 0.664974 1_3:+64782741 Msa0133510:CDS
ACTATGGTCAGAAACTTCAG+AGG 0.696124 1_3:-64782419 None:intergenic
CTGCACAGGAAGCAGCAGAG+AGG 0.705056 1_3:+64784937 Msa0133510:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATGAATATATAAACAA+TGG - chr1_3:64784606-64784625 None:intergenic 10.0%
!!! ATAATTTGTATATAGAACTA+AGG - chr1_3:64783090-64783109 None:intergenic 15.0%
!!! TAGTTCTATATACAAATTAT+AGG + chr1_3:64783090-64783109 Msa0133510:intron 15.0%
!! AATATCATAACATTACTTAA+TGG + chr1_3:64783509-64783528 Msa0133510:intron 15.0%
!!! AAGTATTCTTTTTATAGATA+TGG - chr1_3:64784545-64784564 None:intergenic 15.0%
!! AGATATAGATTATAGTTTAA+CGG - chr1_3:64784789-64784808 None:intergenic 15.0%
!!! TCTTATATATGTTACTTATG+TGG - chr1_3:64783293-64783312 None:intergenic 20.0%
!! TAACGTAATGAGTAAAAAAA+TGG + chr1_3:64783329-64783348 Msa0133510:intron 20.0%
!! AAGTTAGTTAGTTAGTTATT+TGG + chr1_3:64783622-64783641 Msa0133510:intron 20.0%
!!! TTAGTCTATTCTGAAATTTA+TGG + chr1_3:64783766-64783785 Msa0133510:intron 20.0%
!! AAAATAATATCACTATGTGA+TGG - chr1_3:64783921-64783940 None:intergenic 20.0%
!!! TTTTTTTGTCTAACTGTTAA+AGG + chr1_3:64784379-64784398 Msa0133510:intron 20.0%
!! TGAATATATAAACAATGGAA+AGG - chr1_3:64784601-64784620 None:intergenic 20.0%
!!! TGACAGTTATTTTTAAAAAG+CGG + chr1_3:64784700-64784719 Msa0133510:intron 20.0%
! TTGAGAAAGAAGAGAAAAAT+GGG + chr1_3:64782536-64782555 Msa0133510:CDS 25.0%
! TGAATCCTTCATTTCAATAA+AGG + chr1_3:64782576-64782595 Msa0133510:CDS 25.0%
! AACAAAGGTGGAAAATTATA+AGG + chr1_3:64782753-64782772 Msa0133510:CDS 25.0%
! TGGAAAATTATAAGGAGTTA+AGG + chr1_3:64782761-64782780 Msa0133510:CDS 25.0%
! TTATAAGGAGTTAAGGAAAA+AGG + chr1_3:64782768-64782787 Msa0133510:CDS 25.0%
! AAGTTTAACTGAAGAGAATA+GGG + chr1_3:64782819-64782838 Msa0133510:CDS 25.0%
!!! GGATATTAATAACTTGCTTA+GGG + chr1_3:64782840-64782859 Msa0133510:CDS 25.0%
! AAGATAAAGCTCAAATTCAA+AGG - chr1_3:64783156-64783175 None:intergenic 25.0%
! TTACTTATGTGGTATAAATG+GGG - chr1_3:64783282-64783301 None:intergenic 25.0%
! GTTACTTATGTGGTATAAAT+GGG - chr1_3:64783283-64783302 None:intergenic 25.0%
!! TGTTACTTATGTGGTATAAA+TGG - chr1_3:64783284-64783303 None:intergenic 25.0%
! TATCACGTACATTTAACAAA+AGG + chr1_3:64783460-64783479 Msa0133510:intron 25.0%
! ATCACGTACATTTAACAAAA+GGG + chr1_3:64783461-64783480 Msa0133510:intron 25.0%
! GAAAAAAGAGAGAAACAAAA+AGG + chr1_3:64783486-64783505 Msa0133510:intron 25.0%
!!! AAATTTATGGTCATTTTCCA+AGG + chr1_3:64783779-64783798 Msa0133510:intron 25.0%
! AAAACCAAACTAAATGAACT+CGG - chr1_3:64784143-64784162 None:intergenic 25.0%
! GTCATGTTTATTGATCAAAT+AGG - chr1_3:64784316-64784335 None:intergenic 25.0%
!! CTTTTTATAGATATGGCAAA+AGG - chr1_3:64784538-64784557 None:intergenic 25.0%
!!! TCATTTTTGTAGTTGAGAAA+AGG + chr1_3:64784618-64784637 Msa0133510:intron 25.0%
!! CATTTTTGTAGTTGAGAAAA+GGG + chr1_3:64784619-64784638 Msa0133510:intron 25.0%
!! ATTTTTGTAGTTGAGAAAAG+GGG + chr1_3:64784620-64784639 Msa0133510:intron 25.0%
! TTAAAAAGCGGTGATAATAT+AGG + chr1_3:64784712-64784731 Msa0133510:intron 25.0%
! ATAGTATGATATGTTCTTAC+TGG + chr1_3:64784743-64784762 Msa0133510:intron 25.0%
! TAATGTTTGAATGTTCCATT+AGG + chr1_3:64784815-64784834 Msa0133510:intron 25.0%
!! TTTGAGTCTTAATTTTCCAT+TGG - chr1_3:64784892-64784911 None:intergenic 25.0%
! ATTGTTGAGTTTGTACTGAT+TGG - chr1_3:64782383-64782402 None:intergenic 30.0%
CTTTATCTGAATGAAGACTA+TGG - chr1_3:64782438-64782457 None:intergenic 30.0%
CTCAAAACAGTTTCAAAGTA+AGG - chr1_3:64782463-64782482 None:intergenic 30.0%
!!! AACTGTTTTGAGAGAAAAAC+AGG + chr1_3:64782471-64782490 Msa0133510:CDS 30.0%
GTTGAGAAAGAAGAGAAAAA+TGG + chr1_3:64782535-64782554 Msa0133510:CDS 30.0%
AGAAAAATGGGATGATGTTA+AGG + chr1_3:64782548-64782567 Msa0133510:CDS 30.0%
GAAAAATGGGATGATGTTAA+GGG + chr1_3:64782549-64782568 Msa0133510:CDS 30.0%
GGAATCCTTTATTGAAATGA+AGG - chr1_3:64782584-64782603 None:intergenic 30.0%
! ACTCTCTTTTACTCTAGATA+AGG - chr1_3:64782605-64782624 None:intergenic 30.0%
TAGAGTAAAAGAGAGTTTAG+AGG + chr1_3:64782609-64782628 Msa0133510:CDS 30.0%
AGAGTAAAAGAGAGTTTAGA+GGG + chr1_3:64782610-64782629 Msa0133510:CDS 30.0%
AGAGTTAAGTGATGAATCAA+CGG + chr1_3:64782678-64782697 Msa0133510:CDS 30.0%
CAAGTTTAACTGAAGAGAAT+AGG + chr1_3:64782818-64782837 Msa0133510:CDS 30.0%
!! GGGATATTAATAACTTGCTT+AGG + chr1_3:64782839-64782858 Msa0133510:CDS 30.0%
GCATTGGAGAAGAAAATAAA+GGG + chr1_3:64782883-64782902 Msa0133510:CDS 30.0%
TAAAGGGACATGATCATAAA+AGG + chr1_3:64782899-64782918 Msa0133510:CDS 30.0%
TGGTAATAATGTGCAATCAA+TGG + chr1_3:64782987-64783006 Msa0133510:CDS 30.0%
AATAATGTGCAATCAATGGA+TGG + chr1_3:64782991-64783010 Msa0133510:CDS 30.0%
TTCTATGGAATGTGTGAAAA+GGG - chr1_3:64783224-64783243 None:intergenic 30.0%
! GTACATGTAATGGAACTTAT+AGG + chr1_3:64783359-64783378 Msa0133510:intron 30.0%
TTTCTTGGAAGAAAAAACTC+GGG - chr1_3:64783426-64783445 None:intergenic 30.0%
CTTTCTTGGAAGAAAAAACT+CGG - chr1_3:64783427-64783446 None:intergenic 30.0%
ATCATAAGAGAACACTTTCT+TGG - chr1_3:64783441-64783460 None:intergenic 30.0%
TCACGTACATTTAACAAAAG+GGG + chr1_3:64783462-64783481 Msa0133510:intron 30.0%
! AGAGAAGCTAGTTTCTTATA+TGG + chr1_3:64783661-64783680 Msa0133510:intron 30.0%
! TAGAGAGTATGTGTATACAT+AGG + chr1_3:64783689-64783708 Msa0133510:intron 30.0%
TGTAATCTACTTCTTGCAAA+AGG + chr1_3:64783743-64783762 Msa0133510:intron 30.0%
TTCTCAATTGAGTGATTTCA+AGG - chr1_3:64783839-64783858 None:intergenic 30.0%
GTGAGTTCAACATAGTTATA+TGG - chr1_3:64783890-64783909 None:intergenic 30.0%
GCATAATTCATTCATTTGCA+AGG + chr1_3:64783952-64783971 Msa0133510:intron 30.0%
!!! GAGTTCATTTAGTTTGGTTT+TGG + chr1_3:64784142-64784161 Msa0133510:intron 30.0%
ATTATTATGAACTTCATGCC+AGG + chr1_3:64784172-64784191 Msa0133510:intron 30.0%
AGAAAACAAACTCTACATCA+AGG + chr1_3:64784248-64784267 Msa0133510:CDS 30.0%
AATCAAAGAAACAGAAGAAG+AGG + chr1_3:64784421-64784440 Msa0133510:CDS 30.0%
CTAGAACTCAATCTAAAAAC+TGG - chr1_3:64785098-64785117 None:intergenic 30.0%
!! AATATTGCTACCTCTAGAAT+TGG + chr1_3:64785119-64785138 Msa0133510:CDS 30.0%
!! GTTTGTACTGATTGGAAGAT+GGG - chr1_3:64782375-64782394 None:intergenic 35.0%
!! AGTTTGTACTGATTGGAAGA+TGG - chr1_3:64782376-64782395 None:intergenic 35.0%
GAGAGAAAAACAGGATGTAT+TGG + chr1_3:64782480-64782499 Msa0133510:CDS 35.0%
GAAAAACAGGATGTATTGGA+TGG + chr1_3:64782484-64782503 Msa0133510:CDS 35.0%
TGTATTGGATGGAAATGAAG+TGG + chr1_3:64782495-64782514 Msa0133510:CDS 35.0%
! TGTCAGAGAAATAGTTTGTG+AGG + chr1_3:64782651-64782670 Msa0133510:CDS 35.0%
!! TGAATCAACGGTGTTGTTAT+TGG + chr1_3:64782690-64782709 Msa0133510:CDS 35.0%
AAGAGGCAAGGTTAATATCA+AGG + chr1_3:64782713-64782732 Msa0133510:CDS 35.0%
! GGCATTGGAGAAGAAAATAA+AGG + chr1_3:64782882-64782901 Msa0133510:CDS 35.0%
AGTTTGGATTGCAGAAAGTT+GGG + chr1_3:64782944-64782963 Msa0133510:CDS 35.0%
TGAAGTGGAAGTTGTTAGTT+TGG + chr1_3:64783050-64783069 Msa0133510:CDS 35.0%
TTTATCTTGTGTCTCGCATA+GGG + chr1_3:64783168-64783187 Msa0133510:intron 35.0%
CTTCTATGGAATGTGTGAAA+AGG - chr1_3:64783225-64783244 None:intergenic 35.0%
TTCACACATTCCATAGAAGA+TGG + chr1_3:64783226-64783245 Msa0133510:intron 35.0%
TCACACATTCCATAGAAGAT+GGG + chr1_3:64783227-64783246 Msa0133510:intron 35.0%
TTCCATAGAAGATGGGAAAA+TGG + chr1_3:64783234-64783253 Msa0133510:intron 35.0%
! TACCATTTTCCCATCTTCTA+TGG - chr1_3:64783239-64783258 None:intergenic 35.0%
ACTGATAGTAGCAGTATTAC+CGG + chr1_3:64783543-64783562 Msa0133510:intron 35.0%
!! ATTACCGGAAGTATTGTGTT+AGG + chr1_3:64783558-64783577 Msa0133510:intron 35.0%
ACATCCTAACACAATACTTC+CGG - chr1_3:64783565-64783584 None:intergenic 35.0%
CACTCAATTGAGAAGATCTT+TGG + chr1_3:64783845-64783864 Msa0133510:CDS 35.0%
! GAAGATCTTTGGAAGAATCT+AGG + chr1_3:64783856-64783875 Msa0133510:CDS 35.0%
! AGCATGAAGCATTTGTGAAA+GGG - chr1_3:64784018-64784037 None:intergenic 35.0%
! AAGCATGAAGCATTTGTGAA+AGG - chr1_3:64784019-64784038 None:intergenic 35.0%
! CTCGGTATGATTTTGAACTA+TGG - chr1_3:64784125-64784144 None:intergenic 35.0%
CATACCGAGTTCATTTAGTT+TGG + chr1_3:64784136-64784155 Msa0133510:intron 35.0%
AGAAGATAGAGAGAGAATCT+TGG + chr1_3:64784275-64784294 Msa0133510:CDS 35.0%
TGTCTAACTGTTAAAGGTAG+AGG + chr1_3:64784385-64784404 Msa0133510:intron 35.0%
AGCTTGTGAATTGGAAGTTA+AGG + chr1_3:64784460-64784479 Msa0133510:CDS 35.0%
GTCACATACTCAATTCTTGT+TGG - chr1_3:64784684-64784703 None:intergenic 35.0%
TGTTTGAATGTTCCATTAGG+TGG + chr1_3:64784818-64784837 Msa0133510:intron 35.0%
ACAAGAACTGCAGAAAACAA+AGG + chr1_3:64784851-64784870 Msa0133510:CDS 35.0%
CAAGAACTGCAGAAAACAAA+GGG + chr1_3:64784852-64784871 Msa0133510:CDS 35.0%
TTTGGCATTTCCAATTCTAG+AGG - chr1_3:64785132-64785151 None:intergenic 35.0%
CTAGAATTGGAAATGCCAAA+AGG + chr1_3:64785132-64785151 Msa0133510:CDS 35.0%
!!! CATTTCTGGTATCATCCTTT+TGG - chr1_3:64785150-64785169 None:intergenic 35.0%
TGAAGAAAGGATTGCATTTC+TGG - chr1_3:64785164-64785183 None:intergenic 35.0%
! TCAGAAACTTCAGAGGTGTA+GGG - chr1_3:64782415-64782434 None:intergenic 40.0%
ACTATGGTCAGAAACTTCAG+AGG - chr1_3:64782422-64782441 None:intergenic 40.0%
AGAGAGTTTAGAGGGAGAAA+AGG + chr1_3:64782618-64782637 Msa0133510:CDS 40.0%
!! AACGGTGTTGTTATTGGAAG+AGG + chr1_3:64782696-64782715 Msa0133510:CDS 40.0%
! TGTTGTTATTGGAAGAGGCA+AGG + chr1_3:64782701-64782720 Msa0133510:CDS 40.0%
AATATCAAGGCTTGCAACTG+AGG + chr1_3:64782726-64782745 Msa0133510:CDS 40.0%
GAAAAAGGAGAAGAGAGAGT+TGG + chr1_3:64782783-64782802 Msa0133510:CDS 40.0%
! TTTGTTGGAGAAAGAGGCAT+TGG + chr1_3:64782867-64782886 Msa0133510:CDS 40.0%
AACTCAGCAAACTGCAAAAG+TGG - chr1_3:64782928-64782947 None:intergenic 40.0%
!!! CTTTTGCAGTTTGCTGAGTT+TGG + chr1_3:64782928-64782947 Msa0133510:CDS 40.0%
GAGTTTGGATTGCAGAAAGT+TGG + chr1_3:64782943-64782962 Msa0133510:CDS 40.0%
AGTTGGGTTTGGATTCATGA+TGG + chr1_3:64782960-64782979 Msa0133510:CDS 40.0%
GTTGGGTTTGGATTCATGAT+GGG + chr1_3:64782961-64782980 Msa0133510:CDS 40.0%
ATCAATGGATGGTACTTCTG+AGG + chr1_3:64783002-64783021 Msa0133510:CDS 40.0%
CTTTATCTTGTGTCTCGCAT+AGG + chr1_3:64783167-64783186 Msa0133510:intron 40.0%
GATGGGAAAATGGTACATGT+AGG + chr1_3:64783244-64783263 Msa0133510:intron 40.0%
TGGCAACACTGTACATGTAA+TGG + chr1_3:64783349-64783368 Msa0133510:intron 40.0%
CATTATCCTTTCCACTGTTG+AGG - chr1_3:64783805-64783824 None:intergenic 40.0%
TGCAAGGAAGTGAGTGAAAA+AGG + chr1_3:64783968-64783987 Msa0133510:intron 40.0%
! TTTGTTTCTCTGTGAGACAC+TGG - chr1_3:64784215-64784234 None:intergenic 40.0%
ATGGAAGGAAGCTTGTGAAT+TGG + chr1_3:64784451-64784470 Msa0133510:CDS 40.0%
TGTGAATTGGAAGTTAAGGC+TGG + chr1_3:64784464-64784483 Msa0133510:CDS 40.0%
! GATTGAAGAGCGTGACAAAA+TGG + chr1_3:64784496-64784515 Msa0133510:CDS 40.0%
ACTCAAAGAAGAGCTAGCAA+TGG + chr1_3:64784905-64784924 Msa0133510:CDS 40.0%
TTGAAGCTAGCTGATAGTAG+AGG + chr1_3:64784963-64784982 Msa0133510:CDS 40.0%
AAGTGAGTCGACGTATATGT+TGG + chr1_3:64785066-64785085 Msa0133510:CDS 40.0%
CTCAATCTAAAAACTGGCCA+TGG - chr1_3:64785092-64785111 None:intergenic 40.0%
CTGATTGGAAGATGGGTGAT+AGG - chr1_3:64782368-64782387 None:intergenic 45.0%
! TCAGAGGTGTAGGGTGAATT+TGG - chr1_3:64782406-64782425 None:intergenic 45.0%
! GTCAGAAACTTCAGAGGTGT+AGG - chr1_3:64782416-64782435 None:intergenic 45.0%
TGCAACTGAGGTGGAAACAA+AGG + chr1_3:64782738-64782757 Msa0133510:CDS 45.0%
AACTGAGGTGGAAACAAAGG+TGG + chr1_3:64782741-64782760 Msa0133510:CDS 45.0%
! CTTGCTTAGGGTTGCTTTGT+TGG + chr1_3:64782852-64782871 Msa0133510:CDS 45.0%
! GGTTGCTTTGTTGGAGAAAG+AGG + chr1_3:64782861-64782880 Msa0133510:CDS 45.0%
GGATTGCAGAAAGTTGGGTT+TGG + chr1_3:64782949-64782968 Msa0133510:CDS 45.0%
! TTTGGATTCATGATGGGAGG+TGG + chr1_3:64782967-64782986 Msa0133510:CDS 45.0%
AGATAGCAGTGAGTGTGAAG+TGG + chr1_3:64783035-64783054 Msa0133510:CDS 45.0%
! TAGGGCATTTAAGCTCCAGT+AGG + chr1_3:64783186-64783205 Msa0133510:intron 45.0%
GGGTAACAATGTACACCTAC+TGG - chr1_3:64783204-64783223 None:intergenic 45.0%
! ATTTTCCAAGGCCTCAACAG+TGG + chr1_3:64783791-64783810 Msa0133510:intron 45.0%
CAGAAGAAGAGGTAGCTAGA+TGG + chr1_3:64784432-64784451 Msa0133510:CDS 45.0%
AGAAGAGGTAGCTAGATGGA+AGG + chr1_3:64784436-64784455 Msa0133510:CDS 45.0%
!! GCAGAAAACAAAGGGTGCTT+TGG + chr1_3:64784860-64784879 Msa0133510:CDS 45.0%
! GGTGCTTTGGACATATCCAA+TGG + chr1_3:64784873-64784892 Msa0133510:CDS 45.0%
GTAGAGGAGTTGAACTTCGT+CGG + chr1_3:64784979-64784998 Msa0133510:CDS 45.0%
GGAACTAACCAGACAACTAG+AGG + chr1_3:64785010-64785029 Msa0133510:CDS 45.0%
CTCTGATTCCTCTAGTTGTC+TGG - chr1_3:64785021-64785040 None:intergenic 45.0%
ATCAAGGCTTGCAACTGAGG+TGG + chr1_3:64782729-64782748 Msa0133510:CDS 50.0%
! GGGTTTGGATTCATGATGGG+AGG + chr1_3:64782964-64782983 Msa0133510:CDS 50.0%
GTTTCTCTGTGAGACACTGG+AGG - chr1_3:64784212-64784231 None:intergenic 50.0%
GTTTCAGTGCAGCCACCTAA+TGG - chr1_3:64784833-64784852 None:intergenic 50.0%
AATGGCTGCAATAGCTGCAC+AGG + chr1_3:64784923-64784942 Msa0133510:CDS 50.0%
TGAACTTCGTCGGCAGATAG+AGG + chr1_3:64784989-64785008 Msa0133510:CDS 50.0%
GTCGACGTATATGTTGGCCA+TGG + chr1_3:64785072-64785091 Msa0133510:CDS 50.0%
CCTTTCCACTGTTGAGGCCT+TGG - chr1_3:64783799-64783818 None:intergenic 55.0%
CCAAGGCCTCAACAGTGGAA+AGG + chr1_3:64783796-64783815 Msa0133510:intron 55.0%
CTGCACAGGAAGCAGCAGAG+AGG + chr1_3:64784937-64784956 Msa0133510:CDS 60.0%
! GAGGCGCTCTGTGTCTGACC+TGG - chr1_3:64784193-64784212 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 64782355 64785187 64782355 ID=Msa0133510;Name=Msa0133510
chr1_3 mRNA 64782355 64785187 64782355 ID=Msa0133510-mRNA-1;Parent=Msa0133510;Name=Msa0133510-mRNA-1;_AED=0.01;_eAED=0.02;_QI=0|0|0|1|1|1|5|0|457
chr1_3 exon 64782355 64783071 64782355 ID=Msa0133510-mRNA-1:exon:21816;Parent=Msa0133510-mRNA-1
chr1_3 exon 64783801 64783877 64783801 ID=Msa0133510-mRNA-1:exon:21817;Parent=Msa0133510-mRNA-1
chr1_3 exon 64784194 64784305 64784194 ID=Msa0133510-mRNA-1:exon:21818;Parent=Msa0133510-mRNA-1
chr1_3 exon 64784401 64784517 64784401 ID=Msa0133510-mRNA-1:exon:21819;Parent=Msa0133510-mRNA-1
chr1_3 exon 64784837 64785187 64784837 ID=Msa0133510-mRNA-1:exon:21820;Parent=Msa0133510-mRNA-1
chr1_3 CDS 64782355 64783071 64782355 ID=Msa0133510-mRNA-1:cds;Parent=Msa0133510-mRNA-1
chr1_3 CDS 64783801 64783877 64783801 ID=Msa0133510-mRNA-1:cds;Parent=Msa0133510-mRNA-1
chr1_3 CDS 64784194 64784305 64784194 ID=Msa0133510-mRNA-1:cds;Parent=Msa0133510-mRNA-1
chr1_3 CDS 64784401 64784517 64784401 ID=Msa0133510-mRNA-1:cds;Parent=Msa0133510-mRNA-1
chr1_3 CDS 64784837 64785187 64784837 ID=Msa0133510-mRNA-1:cds;Parent=Msa0133510-mRNA-1
Gene Sequence

>Msa0133510

ATGGAACAATCCTATCACCCATCTTCCAATCAGTACAAACTCAACAATCCAAATTCACCCTACACCTCTGAAGTTTCTGACCATAGTCTTCATTCAGATAAAGTTCCTTACTTTGAAACTGTTTTGAGAGAAAAACAGGATGTATTGGATGGAAATGAAGTGGAAGATATCAAAGAAAGAGTTGAGAAAGAAGAGAAAAATGGGATGATGTTAAGGGCTTTTGAATCCTTCATTTCAATAAAGGATTCCTTATCTAGAGTAAAAGAGAGTTTAGAGGGAGAAAAGGATGAAACTTTTGTCAGAGAAATAGTTTGTGAGGAATCAGAGTTAAGTGATGAATCAACGGTGTTGTTATTGGAAGAGGCAAGGTTAATATCAAGGCTTGCAACTGAGGTGGAAACAAAGGTGGAAAATTATAAGGAGTTAAGGAAAAAGGAGAAGAGAGAGTTGGAGAATAGTTTGACAAGTTTAACTGAAGAGAATAGGGATATTAATAACTTGCTTAGGGTTGCTTTGTTGGAGAAAGAGGCATTGGAGAAGAAAATAAAGGGACATGATCATAAAAGGATGCCACTTTTGCAGTTTGCTGAGTTTGGATTGCAGAAAGTTGGGTTTGGATTCATGATGGGAGGTGGTAATAATGTGCAATCAATGGATGGTACTTCTGAGGCTAACAATTCAGATAGCAGTGAGTGTGAAGTGGAAGTTGTTAGTTTGGCCTCAACAGTGGAAAGGATAATGAAGAATCTACGCCTTGAAATCACTCAATTGAGAAGATCTTTGGAAGAATCTAGGTCAGACACAGAGCGCCTCCAGTGTCTCACAGAGAAACAAACAAAAGAGATTGCAGAAAACAAACTCTACATCAAGGAGTTAGAAGATAGAGAGAGAATCTTGGCTCAAAATGTAGAGGAGTTTTTGATTGAAATCAAAGAAACAGAAGAAGAGGTAGCTAGATGGAAGGAAGCTTGTGAATTGGAAGTTAAGGCTGGAAAGAAAGAGATTGAAGAGCGTGACAAAATGGTGGCTGCACTGAAACAAGAACTGCAGAAAACAAAGGGTGCTTTGGACATATCCAATGGAAAATTAAGACTCAAAGAAGAGCTAGCAATGGCTGCAATAGCTGCACAGGAAGCAGCAGAGAGGTCATTGAAGCTAGCTGATAGTAGAGGAGTTGAACTTCGTCGGCAGATAGAGGAACTAACCAGACAACTAGAGGAATCAGAGAAACATGAACGTAATAGTAGTCATAAAGTGAGTCGACGTATATGTTGGCCATGGCCAGTTTTTAGATTGAGTTCTAGCAATATTGCTACCTCTAGAATTGGAAATGCCAAAAGGATGATACCAGAAATGCAATCCTTTCTTCAATGA
Protein sequence

>Msa0133510

MEQSYHPSSNQYKLNNPNSPYTSEVSDHSLHSDKVPYFETVLREKQDVLDGNEVEDIKERVEKEEKNGMMLRAFESFISIKDSLSRVKESLEGEKDETFVREIVCEESELSDESTVLLLEEARLISRLATEVETKVENYKELRKKEKRELENSLTSLTEENRDINNLLRVALLEKEALEKKIKGHDHKRMPLLQFAEFGLQKVGFGFMMGGGNNVQSMDGTSEANNSDSSECEVEVVSLASTVERIMKNLRLEITQLRRSLEESRSDTERLQCLTEKQTKEIAENKLYIKELEDRERILAQNVEEFLIEIKETEEEVARWKEACELEVKAGKKEIEERDKMVAALKQELQKTKGALDISNGKLRLKEELAMAAIAAQEAAERSLKLADSRGVELRRQIEELTRQLEESEKHERNSSHKVSRRICWPWPVFRLSSSNIATSRIGNAKRMIPEMQSFLQ*