AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0134170

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0134170 MtrunA17_Chr1g0204351 95.102 245 11 1 1 245 1 244 3.33e-172 474
Msa0134170 MtrunA17_Chr6g0474671 52.193 228 99 6 21 243 16 238 4.35e-65 202
Msa0134170 MtrunA17_Chr6g0474681 40.223 179 97 3 72 244 2 176 3.45e-34 121
Msa0134170 MtrunA17_Chr1g0204361 35.714 182 101 2 61 231 4 180 3.02e-29 108
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0134170 AT5G51780.2 46.328 177 79 5 74 237 23 196 7.16e-38 131
Msa0134170 AT5G51780.1 46.286 175 78 5 76 237 2 173 1.81e-37 130
Msa0134170 AT4G25400.1 44.444 171 88 3 70 237 19 185 3.26e-36 127
Msa0134170 AT4G25400.2 46.405 153 75 3 70 219 19 167 1.57e-33 120
Msa0134170 AT4G25410.1 43.636 165 83 4 80 234 47 211 3.17e-33 120
Msa0134170 AT5G51790.1 40.541 185 98 5 62 234 9 193 6.63e-32 116
Msa0134170 AT5G51790.3 40.541 185 98 5 62 234 74 258 2.37e-31 116
Msa0134170 AT5G51790.4 41.765 170 87 5 62 219 9 178 2.37e-30 112
Msa0134170 AT5G51790.2 41.765 170 87 5 62 219 74 243 1.01e-29 112
Msa0134170 AT1G62975.1 32.161 199 111 3 63 237 61 259 3.20e-24 97.8
Msa0134170 AT1G12540.1 35.196 179 101 5 76 240 75 252 5.63e-22 91.7
Msa0134170 AT1G62975.2 33.149 181 97 3 63 219 61 241 7.94e-22 91.3

Find 58 sgRNAs with CRISPR-Local

Find 144 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCCCCACTTTCTAAATTTC+TGG 0.103688 1_3:+65678287 Msa0134170:CDS
TCAAAGACTTTCCTAATTAT+TGG 0.193329 1_3:-65677614 None:intergenic
CAAAATTAATTGTGAAGTTT+AGG 0.202471 1_3:+65678784 Msa0134170:three_prime_UTR
GAAGAGGGAGAAGGGATCTA+AGG 0.225427 1_3:-65677943 None:intergenic
ATATATATGTTGGATTGTTT+TGG 0.249216 1_3:-65677578 None:intergenic
TTGGTTTCTGCTTCTGTCTT+GGG 0.275198 1_3:+65678712 Msa0134170:three_prime_UTR
TTCCTTAGCCACCAATAATT+AGG 0.289757 1_3:+65677603 Msa0134170:five_prime_UTR
GAATCCATGTGGGCTCTCTT+TGG 0.295811 1_3:+65678693 Msa0134170:three_prime_UTR
CTGGAACAACTGATGAAATA+TGG 0.304748 1_3:+65678306 Msa0134170:CDS
TATTGAAGATAAGAAGAAAA+TGG 0.318828 1_3:+65677865 Msa0134170:CDS
TTTGGTTTCTGCTTCTGTCT+TGG 0.319200 1_3:+65678711 Msa0134170:three_prime_UTR
TTATATTCTCTCTTTCTAAC+TGG 0.333136 1_3:+65678622 Msa0134170:three_prime_UTR
TCTATATAAAATAATTTGTT+TGG 0.336326 1_3:-65677812 None:intergenic
ATAAGAAGAAAATGGTTCAT+AGG 0.350975 1_3:+65677873 Msa0134170:CDS
ACTAAATGATGGATTCATAT+TGG 0.351963 1_3:-65678546 None:intergenic
AAATCACTTGAAGAAGAATA+TGG 0.357120 1_3:+65678113 Msa0134170:CDS
CGTTGTAAAGGAAACATGTT+TGG 0.371777 1_3:-65677647 None:intergenic
TAAAGGAAACATGTTTGGTT+TGG 0.398708 1_3:-65677642 None:intergenic
TTCCTAATTATTGGTGGCTA+AGG 0.406014 1_3:-65677605 None:intergenic
TATATAGATCGTACAAATCA+TGG 0.422086 1_3:+65677827 Msa0134170:CDS
TACTAGTACTTGCTTTATAG+AGG 0.425326 1_3:+65678260 Msa0134170:CDS
GTGGTTGAGACTATTAGATA+AGG 0.433605 1_3:-65677690 None:intergenic
TGGAAGAACTAAGTTACAAA+AGG 0.434554 1_3:+65678133 Msa0134170:CDS
ATACCTCACACTGCACACTA+TGG 0.439476 1_3:-65678377 None:intergenic
CCACAGTTCTATTATTCTTG+TGG 0.455790 1_3:-65678224 None:intergenic
ATATAAAATAATTTGTTTGG+TGG 0.460635 1_3:-65677809 None:intergenic
AAGAGGGAGAAGGGATCTAA+GGG 0.467872 1_3:-65677942 None:intergenic
TAATCTGAACAACTAAATGA+TGG 0.468144 1_3:-65678557 None:intergenic
TAAGAAGAAAATGGTTCATA+GGG 0.475156 1_3:+65677874 Msa0134170:CDS
TAGATCTAACTGAGCTAAGA+AGG 0.478104 1_3:+65678516 Msa0134170:CDS
TTTACAACGATGCAATGAAC+TGG 0.483997 1_3:+65677661 Msa0134170:CDS
AGTTCATTGCATCGTTGTAA+AGG 0.484172 1_3:-65677659 None:intergenic
AAAATAATTTGTTTGGTGGC+CGG 0.487549 1_3:-65677805 None:intergenic
AAGACTTTCCTAATTATTGG+TGG 0.493076 1_3:-65677611 None:intergenic
TGGTTGAGACTATTAGATAA+GGG 0.494385 1_3:-65677689 None:intergenic
CTAAGGAATCATATATATGT+TGG 0.508479 1_3:-65677588 None:intergenic
GTTCCAGAAATTTAGAAAGT+GGG 0.518377 1_3:-65678290 None:intergenic
TTTATCTGATCAAATGAATG+AGG 0.525114 1_3:+65678077 Msa0134170:CDS
TGTTCCAGAAATTTAGAAAG+TGG 0.525663 1_3:-65678291 None:intergenic
CACAAGAATAATAGAACTGT+GGG 0.526811 1_3:+65678225 Msa0134170:CDS
GGAAGAACTAAGTTACAAAA+GGG 0.531740 1_3:+65678134 Msa0134170:CDS
GAAGCAAAGGAGACAAGAAA+TGG 0.533586 1_3:+65677904 Msa0134170:CDS
TCTCCCTCTTCACTTCATCA+AGG 0.533778 1_3:+65677955 Msa0134170:CDS
TCACCTTGATGAAGTGAAGA+GGG 0.538188 1_3:-65677958 None:intergenic
GATGAAGTGAAGAGGGAGAA+GGG 0.546212 1_3:-65677951 None:intergenic
CCCACAACATCACAAAATCT+CGG 0.553306 1_3:+65677715 Msa0134170:CDS
AGTACTTGCTTTATAGAGGA+AGG 0.558027 1_3:+65678264 Msa0134170:CDS
TTCCAGAAATTTAGAAAGTG+GGG 0.570258 1_3:-65678289 None:intergenic
ATAGGGAAATTGAGAAGCAA+AGG 0.575199 1_3:+65677891 Msa0134170:CDS
TGATGAAGTGAAGAGGGAGA+AGG 0.577558 1_3:-65677952 None:intergenic
CTCAGTTAGATCTACACTGT+CGG 0.580625 1_3:-65678507 None:intergenic
GAAACCAAAGAGAGCCCACA+TGG 0.590718 1_3:-65678697 None:intergenic
TTCACCTTGATGAAGTGAAG+AGG 0.603402 1_3:-65677959 None:intergenic
TGTAAATATTGAATCCATGT+GGG 0.608389 1_3:+65678683 Msa0134170:three_prime_UTR
CCACAAGAATAATAGAACTG+TGG 0.617517 1_3:+65678224 Msa0134170:CDS
ACTCCATAGTGTGCAGTGTG+AGG 0.655288 1_3:+65678374 Msa0134170:CDS
ATGTAAATATTGAATCCATG+TGG 0.661065 1_3:+65678682 Msa0134170:three_prime_UTR
TAGTGAAACTACAAGAAACA+TGG 0.661078 1_3:-65678200 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCTATATAAAATAATTTGTT+TGG - chr1_3:65677815-65677834 None:intergenic 10.0%
!!! TCTATATAAAATAATTTGTT+TGG - chr1_3:65677815-65677834 None:intergenic 10.0%
!! TTAAAAAAATAGATACTAAC+CGG + chr1_3:65677786-65677805 Msa0134170:CDS 15.0%
!!! ATATAAAATAATTTGTTTGG+TGG - chr1_3:65677812-65677831 None:intergenic 15.0%
!!! TTTAATCATTAAAGTTTTCT+AGG + chr1_3:65677997-65678016 Msa0134170:intron 15.0%
!! TTAAAAAAATAGATACTAAC+CGG + chr1_3:65677786-65677805 Msa0134170:CDS 15.0%
!!! ATATAAAATAATTTGTTTGG+TGG - chr1_3:65677812-65677831 None:intergenic 15.0%
!!! TTTAATCATTAAAGTTTTCT+AGG + chr1_3:65677997-65678016 Msa0134170:intron 15.0%
!! TATTGAAGATAAGAAGAAAA+TGG + chr1_3:65677865-65677884 Msa0134170:CDS 20.0%
!! TTATCATCTGTTATAACTTT+AGG + chr1_3:65678020-65678039 Msa0134170:intron 20.0%
!! TATCATCTGTTATAACTTTA+GGG + chr1_3:65678021-65678040 Msa0134170:intron 20.0%
!!! TTATTTATTGTTTCTCTAAC+AGG + chr1_3:65678044-65678063 Msa0134170:intron 20.0%
!!! TATTTATTGTTTCTCTAACA+GGG + chr1_3:65678045-65678064 Msa0134170:intron 20.0%
!!! AAACATGGCTTTTATTTTTA+AGG - chr1_3:65678188-65678207 None:intergenic 20.0%
!!! TATGACAATTTTTTGTAAAC+AGG - chr1_3:65678601-65678620 None:intergenic 20.0%
!! CAAAATTAATTGTGAAGTTT+AGG + chr1_3:65678784-65678803 Msa0134170:three_prime_UTR 20.0%
!! TATTGAAGATAAGAAGAAAA+TGG + chr1_3:65677865-65677884 Msa0134170:CDS 20.0%
!! TTATCATCTGTTATAACTTT+AGG + chr1_3:65678020-65678039 Msa0134170:intron 20.0%
!! TATCATCTGTTATAACTTTA+GGG + chr1_3:65678021-65678040 Msa0134170:intron 20.0%
!!! TTATTTATTGTTTCTCTAAC+AGG + chr1_3:65678044-65678063 Msa0134170:intron 20.0%
!!! TATTTATTGTTTCTCTAACA+GGG + chr1_3:65678045-65678064 Msa0134170:intron 20.0%
!!! AAACATGGCTTTTATTTTTA+AGG - chr1_3:65678188-65678207 None:intergenic 20.0%
!!! TATGACAATTTTTTGTAAAC+AGG - chr1_3:65678601-65678620 None:intergenic 20.0%
!! CAAAATTAATTGTGAAGTTT+AGG + chr1_3:65678784-65678803 Msa0134170:three_prime_UTR 20.0%
! CTAAGGAATCATATATATGT+TGG - chr1_3:65677591-65677610 None:intergenic 25.0%
! TCAAAGACTTTCCTAATTAT+TGG - chr1_3:65677617-65677636 None:intergenic 25.0%
! TATATAGATCGTACAAATCA+TGG + chr1_3:65677827-65677846 Msa0134170:CDS 25.0%
! ATAAGAAGAAAATGGTTCAT+AGG + chr1_3:65677873-65677892 Msa0134170:CDS 25.0%
! TAAGAAGAAAATGGTTCATA+GGG + chr1_3:65677874-65677893 Msa0134170:CDS 25.0%
! TTTATCTGATCAAATGAATG+AGG + chr1_3:65678077-65678096 Msa0134170:CDS 25.0%
! AAATCACTTGAAGAAGAATA+TGG + chr1_3:65678113-65678132 Msa0134170:CDS 25.0%
!!! ACTAAATGATGGATTCATAT+TGG - chr1_3:65678549-65678568 None:intergenic 25.0%
! TAATCTGAACAACTAAATGA+TGG - chr1_3:65678560-65678579 None:intergenic 25.0%
! TTATATTCTCTCTTTCTAAC+TGG + chr1_3:65678622-65678641 Msa0134170:three_prime_UTR 25.0%
! ATGTAAATATTGAATCCATG+TGG + chr1_3:65678682-65678701 Msa0134170:three_prime_UTR 25.0%
! TGTAAATATTGAATCCATGT+GGG + chr1_3:65678683-65678702 Msa0134170:three_prime_UTR 25.0%
! CTAAGGAATCATATATATGT+TGG - chr1_3:65677591-65677610 None:intergenic 25.0%
! TCAAAGACTTTCCTAATTAT+TGG - chr1_3:65677617-65677636 None:intergenic 25.0%
! TATATAGATCGTACAAATCA+TGG + chr1_3:65677827-65677846 Msa0134170:CDS 25.0%
! ATAAGAAGAAAATGGTTCAT+AGG + chr1_3:65677873-65677892 Msa0134170:CDS 25.0%
! TAAGAAGAAAATGGTTCATA+GGG + chr1_3:65677874-65677893 Msa0134170:CDS 25.0%
! TTTATCTGATCAAATGAATG+AGG + chr1_3:65678077-65678096 Msa0134170:CDS 25.0%
! AAATCACTTGAAGAAGAATA+TGG + chr1_3:65678113-65678132 Msa0134170:CDS 25.0%
!!! ACTAAATGATGGATTCATAT+TGG - chr1_3:65678549-65678568 None:intergenic 25.0%
! TAATCTGAACAACTAAATGA+TGG - chr1_3:65678560-65678579 None:intergenic 25.0%
! TTATATTCTCTCTTTCTAAC+TGG + chr1_3:65678622-65678641 Msa0134170:three_prime_UTR 25.0%
! ATGTAAATATTGAATCCATG+TGG + chr1_3:65678682-65678701 Msa0134170:three_prime_UTR 25.0%
! TGTAAATATTGAATCCATGT+GGG + chr1_3:65678683-65678702 Msa0134170:three_prime_UTR 25.0%
AAGACTTTCCTAATTATTGG+TGG - chr1_3:65677614-65677633 None:intergenic 30.0%
TAAAGGAAACATGTTTGGTT+TGG - chr1_3:65677645-65677664 None:intergenic 30.0%
TGGTTGAGACTATTAGATAA+GGG - chr1_3:65677692-65677711 None:intergenic 30.0%
!! AAAATAATTTGTTTGGTGGC+CGG - chr1_3:65677808-65677827 None:intergenic 30.0%
TGGAAGAACTAAGTTACAAA+AGG + chr1_3:65678133-65678152 Msa0134170:CDS 30.0%
GGAAGAACTAAGTTACAAAA+GGG + chr1_3:65678134-65678153 Msa0134170:CDS 30.0%
!!! TGGCTTTTATTTTTAAGGCT+AGG - chr1_3:65678183-65678202 None:intergenic 30.0%
TAGTGAAACTACAAGAAACA+TGG - chr1_3:65678203-65678222 None:intergenic 30.0%
CACAAGAATAATAGAACTGT+GGG + chr1_3:65678225-65678244 Msa0134170:CDS 30.0%
TACTAGTACTTGCTTTATAG+AGG + chr1_3:65678260-65678279 Msa0134170:CDS 30.0%
TTCCAGAAATTTAGAAAGTG+GGG - chr1_3:65678292-65678311 None:intergenic 30.0%
GTTCCAGAAATTTAGAAAGT+GGG - chr1_3:65678293-65678312 None:intergenic 30.0%
TGTTCCAGAAATTTAGAAAG+TGG - chr1_3:65678294-65678313 None:intergenic 30.0%
! TGCTTTTCTATTCAAGTCAA+TGG + chr1_3:65678345-65678364 Msa0134170:CDS 30.0%
AAGACTTTCCTAATTATTGG+TGG - chr1_3:65677614-65677633 None:intergenic 30.0%
TAAAGGAAACATGTTTGGTT+TGG - chr1_3:65677645-65677664 None:intergenic 30.0%
TGGTTGAGACTATTAGATAA+GGG - chr1_3:65677692-65677711 None:intergenic 30.0%
!! AAAATAATTTGTTTGGTGGC+CGG - chr1_3:65677808-65677827 None:intergenic 30.0%
TGGAAGAACTAAGTTACAAA+AGG + chr1_3:65678133-65678152 Msa0134170:CDS 30.0%
GGAAGAACTAAGTTACAAAA+GGG + chr1_3:65678134-65678153 Msa0134170:CDS 30.0%
!!! TGGCTTTTATTTTTAAGGCT+AGG - chr1_3:65678183-65678202 None:intergenic 30.0%
TAGTGAAACTACAAGAAACA+TGG - chr1_3:65678203-65678222 None:intergenic 30.0%
CACAAGAATAATAGAACTGT+GGG + chr1_3:65678225-65678244 Msa0134170:CDS 30.0%
TACTAGTACTTGCTTTATAG+AGG + chr1_3:65678260-65678279 Msa0134170:CDS 30.0%
TTCCAGAAATTTAGAAAGTG+GGG - chr1_3:65678292-65678311 None:intergenic 30.0%
GTTCCAGAAATTTAGAAAGT+GGG - chr1_3:65678293-65678312 None:intergenic 30.0%
TGTTCCAGAAATTTAGAAAG+TGG - chr1_3:65678294-65678313 None:intergenic 30.0%
! TGCTTTTCTATTCAAGTCAA+TGG + chr1_3:65678345-65678364 Msa0134170:CDS 30.0%
TTCCTTAGCCACCAATAATT+AGG + chr1_3:65677603-65677622 Msa0134170:five_prime_UTR 35.0%
! TTCCTAATTATTGGTGGCTA+AGG - chr1_3:65677608-65677627 None:intergenic 35.0%
CGTTGTAAAGGAAACATGTT+TGG - chr1_3:65677650-65677669 None:intergenic 35.0%
AGTTCATTGCATCGTTGTAA+AGG - chr1_3:65677662-65677681 None:intergenic 35.0%
TTTACAACGATGCAATGAAC+TGG + chr1_3:65677661-65677680 Msa0134170:CDS 35.0%
GTGGTTGAGACTATTAGATA+AGG - chr1_3:65677693-65677712 None:intergenic 35.0%
ATAGGGAAATTGAGAAGCAA+AGG + chr1_3:65677891-65677910 Msa0134170:CDS 35.0%
CCACAGTTCTATTATTCTTG+TGG - chr1_3:65678227-65678246 None:intergenic 35.0%
CCACAAGAATAATAGAACTG+TGG + chr1_3:65678224-65678243 Msa0134170:CDS 35.0%
AGTACTTGCTTTATAGAGGA+AGG + chr1_3:65678264-65678283 Msa0134170:CDS 35.0%
CTGGAACAACTGATGAAATA+TGG + chr1_3:65678306-65678325 Msa0134170:CDS 35.0%
! TATTGAGCTTGATGATTCAC+AGG + chr1_3:65678475-65678494 Msa0134170:intron 35.0%
TAGATCTAACTGAGCTAAGA+AGG + chr1_3:65678516-65678535 Msa0134170:CDS 35.0%
TTCCTTAGCCACCAATAATT+AGG + chr1_3:65677603-65677622 Msa0134170:five_prime_UTR 35.0%
! TTCCTAATTATTGGTGGCTA+AGG - chr1_3:65677608-65677627 None:intergenic 35.0%
CGTTGTAAAGGAAACATGTT+TGG - chr1_3:65677650-65677669 None:intergenic 35.0%
AGTTCATTGCATCGTTGTAA+AGG - chr1_3:65677662-65677681 None:intergenic 35.0%
TTTACAACGATGCAATGAAC+TGG + chr1_3:65677661-65677680 Msa0134170:CDS 35.0%
GTGGTTGAGACTATTAGATA+AGG - chr1_3:65677693-65677712 None:intergenic 35.0%
ATAGGGAAATTGAGAAGCAA+AGG + chr1_3:65677891-65677910 Msa0134170:CDS 35.0%
CCACAGTTCTATTATTCTTG+TGG - chr1_3:65678227-65678246 None:intergenic 35.0%
CCACAAGAATAATAGAACTG+TGG + chr1_3:65678224-65678243 Msa0134170:CDS 35.0%
AGTACTTGCTTTATAGAGGA+AGG + chr1_3:65678264-65678283 Msa0134170:CDS 35.0%
CTGGAACAACTGATGAAATA+TGG + chr1_3:65678306-65678325 Msa0134170:CDS 35.0%
! TATTGAGCTTGATGATTCAC+AGG + chr1_3:65678475-65678494 Msa0134170:intron 35.0%
TAGATCTAACTGAGCTAAGA+AGG + chr1_3:65678516-65678535 Msa0134170:CDS 35.0%
! AGATTTTGTGATGTTGTGGG+TGG - chr1_3:65677715-65677734 None:intergenic 40.0%
! CCGAGATTTTGTGATGTTGT+GGG - chr1_3:65677718-65677737 None:intergenic 40.0%
CCCACAACATCACAAAATCT+CGG + chr1_3:65677715-65677734 Msa0134170:CDS 40.0%
! TCCGAGATTTTGTGATGTTG+TGG - chr1_3:65677719-65677738 None:intergenic 40.0%
GAAGCAAAGGAGACAAGAAA+TGG + chr1_3:65677904-65677923 Msa0134170:CDS 40.0%
TCACCTTGATGAAGTGAAGA+GGG - chr1_3:65677961-65677980 None:intergenic 40.0%
TTCACCTTGATGAAGTGAAG+AGG - chr1_3:65677962-65677981 None:intergenic 40.0%
TGCCCCACTTTCTAAATTTC+TGG + chr1_3:65678287-65678306 Msa0134170:CDS 40.0%
CTCAGTTAGATCTACACTGT+CGG - chr1_3:65678510-65678529 None:intergenic 40.0%
! TTTGGTTTCTGCTTCTGTCT+TGG + chr1_3:65678711-65678730 Msa0134170:three_prime_UTR 40.0%
TTGGTTTCTGCTTCTGTCTT+GGG + chr1_3:65678712-65678731 Msa0134170:three_prime_UTR 40.0%
! AGATTTTGTGATGTTGTGGG+TGG - chr1_3:65677715-65677734 None:intergenic 40.0%
! CCGAGATTTTGTGATGTTGT+GGG - chr1_3:65677718-65677737 None:intergenic 40.0%
CCCACAACATCACAAAATCT+CGG + chr1_3:65677715-65677734 Msa0134170:CDS 40.0%
! TCCGAGATTTTGTGATGTTG+TGG - chr1_3:65677719-65677738 None:intergenic 40.0%
GAAGCAAAGGAGACAAGAAA+TGG + chr1_3:65677904-65677923 Msa0134170:CDS 40.0%
TCACCTTGATGAAGTGAAGA+GGG - chr1_3:65677961-65677980 None:intergenic 40.0%
TTCACCTTGATGAAGTGAAG+AGG - chr1_3:65677962-65677981 None:intergenic 40.0%
TGCCCCACTTTCTAAATTTC+TGG + chr1_3:65678287-65678306 Msa0134170:CDS 40.0%
CTCAGTTAGATCTACACTGT+CGG - chr1_3:65678510-65678529 None:intergenic 40.0%
! TTTGGTTTCTGCTTCTGTCT+TGG + chr1_3:65678711-65678730 Msa0134170:three_prime_UTR 40.0%
TTGGTTTCTGCTTCTGTCTT+GGG + chr1_3:65678712-65678731 Msa0134170:three_prime_UTR 40.0%
! TTTTGTGATGTTGTGGGTGG+TGG - chr1_3:65677712-65677731 None:intergenic 45.0%
AAGAGGGAGAAGGGATCTAA+GGG - chr1_3:65677945-65677964 None:intergenic 45.0%
GATGAAGTGAAGAGGGAGAA+GGG - chr1_3:65677954-65677973 None:intergenic 45.0%
TGATGAAGTGAAGAGGGAGA+AGG - chr1_3:65677955-65677974 None:intergenic 45.0%
TCTCCCTCTTCACTTCATCA+AGG + chr1_3:65677955-65677974 Msa0134170:CDS 45.0%
ATACCTCACACTGCACACTA+TGG - chr1_3:65678380-65678399 None:intergenic 45.0%
! TTTTGTGATGTTGTGGGTGG+TGG - chr1_3:65677712-65677731 None:intergenic 45.0%
AAGAGGGAGAAGGGATCTAA+GGG - chr1_3:65677945-65677964 None:intergenic 45.0%
GATGAAGTGAAGAGGGAGAA+GGG - chr1_3:65677954-65677973 None:intergenic 45.0%
TGATGAAGTGAAGAGGGAGA+AGG - chr1_3:65677955-65677974 None:intergenic 45.0%
TCTCCCTCTTCACTTCATCA+AGG + chr1_3:65677955-65677974 Msa0134170:CDS 45.0%
ATACCTCACACTGCACACTA+TGG - chr1_3:65678380-65678399 None:intergenic 45.0%
GAAGAGGGAGAAGGGATCTA+AGG - chr1_3:65677946-65677965 None:intergenic 50.0%
ACTCCATAGTGTGCAGTGTG+AGG + chr1_3:65678374-65678393 Msa0134170:CDS 50.0%
GAATCCATGTGGGCTCTCTT+TGG + chr1_3:65678693-65678712 Msa0134170:three_prime_UTR 50.0%
GAAACCAAAGAGAGCCCACA+TGG - chr1_3:65678700-65678719 None:intergenic 50.0%
GAAGAGGGAGAAGGGATCTA+AGG - chr1_3:65677946-65677965 None:intergenic 50.0%
ACTCCATAGTGTGCAGTGTG+AGG + chr1_3:65678374-65678393 Msa0134170:CDS 50.0%
GAATCCATGTGGGCTCTCTT+TGG + chr1_3:65678693-65678712 Msa0134170:three_prime_UTR 50.0%
GAAACCAAAGAGAGCCCACA+TGG - chr1_3:65678700-65678719 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 65677580 65678850 65677580 ID=Msa0134170;Name=Msa0134170
chr1_3 mRNA 65677580 65678850 65677580 ID=Msa0134170-mRNA-1;Parent=Msa0134170;Name=Msa0134170-mRNA-1;_AED=0.02;_eAED=0.02;_QI=73|1|1|1|1|1|3|270|245
chr1_3 exon 65677580 65677976 65677580 ID=Msa0134170-mRNA-1:exon:22289;Parent=Msa0134170-mRNA-1
chr1_3 exon 65678066 65678395 65678066 ID=Msa0134170-mRNA-1:exon:22290;Parent=Msa0134170-mRNA-1
chr1_3 exon 65678497 65678850 65678497 ID=Msa0134170-mRNA-1:exon:22291;Parent=Msa0134170-mRNA-1
chr1_3 five_prime_UTR 65677580 65677652 65677580 ID=Msa0134170-mRNA-1:five_prime_utr;Parent=Msa0134170-mRNA-1
chr1_3 CDS 65677653 65677976 65677653 ID=Msa0134170-mRNA-1:cds;Parent=Msa0134170-mRNA-1
chr1_3 CDS 65678066 65678395 65678066 ID=Msa0134170-mRNA-1:cds;Parent=Msa0134170-mRNA-1
chr1_3 CDS 65678497 65678580 65678497 ID=Msa0134170-mRNA-1:cds;Parent=Msa0134170-mRNA-1
chr1_3 three_prime_UTR 65678581 65678850 65678581 ID=Msa0134170-mRNA-1:three_prime_utr;Parent=Msa0134170-mRNA-1
Gene Sequence

>Msa0134170

ATGTTTCCTTTACAACGATGCAATGAACTGGCGAAACCCTTATCTAATAGTCTCAACCACCACCCACAACATCACAAAATCTCGGAAGATCTGATTCTTGATGATTGTGATTCTCTTGTAATTGATTTTTCTCTTAAAAAAATAGATACTAACCGGCCACCAAACAAATTATTTTATATAGATCGTACAAATCATGGTAGTAATCGTAATTCTATTGAAGATAAGAAGAAAATGGTTCATAGGGAAATTGAGAAGCAAAGGAGACAAGAAATGGCTACACTTCATGCTTCCCTTAGATCCCTTCTCCCTCTTCACTTCATCAAGGGAAAACGTTCTTTATCTGATCAAATGAATGAGGCAGTGAATTACATAAATCACTTGAAGAAGAATATGGAAGAACTAAGTTACAAAAGGGATGAACTGAAAAAACTCTCTAATCCTAGCCTTAAAAATAAAAGCCATGTTTCTTGTAGTTTCACTATCCACAAGAATAATAGAACTGTGGGAATTGAAATCAGTACTAGTACTTGCTTTATAGAGGAAGGTGCCCCACTTTCTAAATTTCTGGAACAACTGATGAAATATGGACTTGATGTTGTTAGCTGCTTTTCTATTCAAGTCAATGGAAGATTACTCCATAGTGTGCAGTGTGAGGTTATCGATTCCGACAGTGTAGATCTAACTGAGCTAAGAAGGAATTTTTCCAATATGAATCCATCATTTAGTTGTTCAGATTAA
Protein sequence

>Msa0134170

MFPLQRCNELAKPLSNSLNHHPQHHKISEDLILDDCDSLVIDFSLKKIDTNRPPNKLFYIDRTNHGSNRNSIEDKKKMVHREIEKQRRQEMATLHASLRSLLPLHFIKGKRSLSDQMNEAVNYINHLKKNMEELSYKRDELKKLSNPSLKNKSHVSCSFTIHKNNRTVGIEISTSTCFIEEGAPLSKFLEQLMKYGLDVVSCFSIQVNGRLLHSVQCEVIDSDSVDLTELRRNFSNMNPSFSCSD*