AlfalfaGEDB | Msa0135650

M. sativa cultivar zhongmu-4 / Msa0135650

KEGG: -
GO: -
PFAM: PF13359.9

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0135650	XP_024631836.2	86.967	399	11	1	1	358	1	399	0.0	707

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0135650	A0A396JPB9	70.449	379	71	1	20	357	27	405	0.0	563

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0135650	Msa0645290	0.988593	1.248668e-174	-8.615850e-47
Msa0135650	Msa0767730	0.883309	5.003597e-71	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0135650	MtrunA17_Chr1g0172731	70.449	379	71	1	20	357	27	405	0.0	563
Msa0135650	MtrunA17_Chr1g0190171	69.657	379	74	1	20	357	27	405	0.0	559
Msa0135650	MtrunA17_Chr2g0321401	61.111	252	51	2	1	205	1	252	2.12e-104	308
Msa0135650	MtrunA17_Chr8g0334561	60.079	253	53	2	1	205	1	253	6.05e-104	306
Msa0135650	MtrunA17_Chr1g0182341	44.099	322	153	5	13	309	15	334	2.03e-86	266
Msa0135650	MtrunA17_Chr2g0285471	60.870	184	31	1	63	205	1	184	1.59e-71	221
Msa0135650	MtrunA17_Chr6g0478531	36.494	348	170	7	35	339	15	354	1.69e-64	209
Msa0135650	MtrunA17_Chr6g0477141	37.082	329	161	4	12	297	93	418	3.49e-62	206
Msa0135650	MtrunA17_Chr8g0391981	33.151	365	181	8	18	337	36	382	8.82e-51	174
Msa0135650	MtrunA17_Chr7g0226311	36.025	322	154	7	59	336	9	322	3.99e-50	171

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0135650	AT5G41980.1	34.218	339	163	9	33	330	15	334	3.59e-53	180
Msa0135650	AT1G43722.1	32.051	312	157	7	4	267	16	320	3.15e-42	150
Msa0135650	AT5G28730.1	34.545	220	117	6	33	232	2	214	4.48e-33	125
Msa0135650	AT5G35695.1	41.912	136	61	4	174	305	30	151	4.56e-26	103
Msa0135650	AT4G10890.3	34.211	190	90	2	81	270	6	160	2.77e-23	101
Msa0135650	AT4G10890.1	34.211	190	90	2	81	270	6	160	2.77e-23	101
Msa0135650	AT4G10890.2	34.211	190	90	2	81	270	6	160	2.77e-23	101
Msa0135650	AT3G30525.1	40.171	117	61	3	176	284	43	158	6.32e-19	83.6

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Find 62 sgRNAs with CRISPR-Local

Find 190 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGCCCACTAATCACATAG+GGG	0.730444	1_3:-67329727	Msa0135650:CDS
AACATCTCATAACAACGAGT+TGG	0.551166	1_3:+67329807	None:intergenic
AACATGATTCATCTCAGATG+AGG	0.624817	1_3:+67328774	None:intergenic
AATGCTATAGGAGCAATCGA+TGG	0.684778	1_3:-67329477	Msa0135650:intron
ACGATAATCATCATGATCAA+GGG	0.621055	1_3:+67328840	None:intergenic
ACTTGTTGTTGGACATGGAG+TGG	0.572106	1_3:-67329679	Msa0135650:CDS
ATAATATTAATACCTGGAGG+AGG	0.608585	1_3:+67329255	None:intergenic
ATATGGACGTCGATTTCAAT+CGG	0.561835	1_3:-67328882	Msa0135650:CDS
ATGTATTATTTGGTGGATGC+CGG	0.486645	1_3:-67329171	Msa0135650:CDS
ATTATTATCACTCAAGTTTA+AGG	0.112859	1_3:-67329044	Msa0135650:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTGTTATGAGATGTTTCGAA+TGG	-	chr1_3:67328902-67328921	Msa0135650:CDS	30.0%
TGCACTAAATTAGTTGATCA+TGG	-	chr1_3:67328948-67328967	Msa0135650:CDS	30.0%
TTAAAAAATGGCCAATATCG+TGG	+	chr1_3:67329203-67329222	None:intergenic	30.0%
AGTGACCAAACTAGATTTAT+TGG	-	chr1_3:67329275-67329294	Msa0135650:CDS	30.0%
TTCGTCCAATAAATCTAGTT+TGG	+	chr1_3:67329283-67329302	None:intergenic	30.0%
AACTAGATTTATTGGACGAA+AGG	-	chr1_3:67329283-67329302	Msa0135650:CDS	30.0%
ACTAGATTTATTGGACGAAA+GGG	-	chr1_3:67329284-67329303	Msa0135650:CDS	30.0%
ATATCCGACACAAAATGTAA+TGG	-	chr1_3:67329307-67329326	Msa0135650:CDS	30.0%
ATGTAATGGTTGTATGTGAT+TGG	-	chr1_3:67329321-67329340	Msa0135650:CDS	30.0%
ATAATATTAATACCTGGAGG+AGG	+	chr1_3:67329449-67329468	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_3	gene	67328726	67329997	67328726	ID=Msa0135650;Name=Msa0135650
chr1_3	mRNA	67328726	67329997	67328726	ID=Msa0135650-mRNA-1;Parent=Msa0135650;Name=Msa0135650-mRNA-1;_AED=0.21;_eAED=0.21;_QI=0\|0.5\|0\|1\|1\|1\|3\|0\|358
chr1_3	exon	67329478	67329997	67329478	ID=Msa0135650-mRNA-1:exon:23127;Parent=Msa0135650-mRNA-1
chr1_3	exon	67329268	67329354	67329268	ID=Msa0135650-mRNA-1:exon:23126;Parent=Msa0135650-mRNA-1
chr1_3	exon	67328726	67329195	67328726	ID=Msa0135650-mRNA-1:exon:23125;Parent=Msa0135650-mRNA-1
chr1_3	CDS	67329478	67329997	67329478	ID=Msa0135650-mRNA-1:cds;Parent=Msa0135650-mRNA-1
chr1_3	CDS	67329268	67329354	67329268	ID=Msa0135650-mRNA-1:cds;Parent=Msa0135650-mRNA-1
chr1_3	CDS	67328726	67329195	67328726	ID=Msa0135650-mRNA-1:cds;Parent=Msa0135650-mRNA-1

Gene Sequence

>Msa0135650

ATGAACTATTGGAATGAAGAATCAAATGAAGACGATTATTTTTTTGAACAAGCTTCTTTAGTGGCTGCACTGATGGGTGAGTATGGCGTGAAACATTTATGTAAAGAGCCATGTAGAACTAGTGAGCTAACAGGGCATGCATGGGTGAAAGAAGTGTTACAAGGGAATCCAACTCGTTGTTATGAGATGTTTCGAATGGAAAAACCTATTTTTCATAAACTTTGCACTAAATTAGTTGATCATGGTTTAAAGCCCACTAATCACATAGGGGTAGAAGAGATGGTTGCAATGTTCTTACTTGTTGTTGGACATGGAGTGGGTAATAGAATGATTCAAGAAAGGTTCCAACACTCAGGAGAAACTGTGAGTAGACGTTTTCATGATGTGTTAGTTGCTTGCTTGAGTTTGTCCATCGAATATATAAAGCCTCAAGATCCTTTGTTCAGTGATAGTCATGCAAAAATTCAAAGTGATCCACGATATTGGCCATTTTTTAAGAATGCTATAGGAGCAATCGATGCTGGATGGGAAGGTACTGCCCATGATGCTCGTGTTTTTGACCATGCTCTCACAAATGCAAATCTAAATTTTCCACATCCTCCTCCAGGTATGTATTATTTGGTGGATGCCGGTTATCCAACACCGATGGGGTATCTTGGTCCATACAGATGTGAACGTTATCACCTCCCTGATTTTCGGCGTTCCCATGGGTTTGAAAATAACAACGAGGTATTCAATTATTATCACTCAAGTTTAAGGTGCACAATAGAAAGAACTTTTGGTGTATGGAAGAATAGATTTGCAATTCTACGACGCATGCCTAAATTCACAATTAAAACACAAGTTGAAGTCGTTGTTGCAACAATGGCTATACATAACTTTATTAGAAGGAATGCTGATATGGACGTCGATTTCAATCGGTATGAGGATGAAGACATAACCCTTGATCATGATGATTATCGTAGACCAGTTAATTTGGATTCATCTCAAAATTTAAATATAGCTTCCTCATCTGAGATGAATCATGTTCGAAACTCGGTTCGAGATCAAATTATAGAATTCAAGAAAAATCATTAG

Protein sequence

>Msa0135650

MNYWNEESNEDDYFFEQASLVAALMGEYGVKHLCKEPCRTSELTGHAWVKEVLQGNPTRCYEMFRMEKPIFHKLCTKLVDHGLKPTNHIGVEEMVAMFLLVVGHGVGNRMIQERFQHSGETVSRRFHDVLVACLSLSIEYIKPQDPLFSDSHAKIQSDPRYWPFFKNAIGAIDAGWEGTAHDARVFDHALTNANLNFPHPPPGMYYLVDAGYPTPMGYLGPYRCERYHLPDFRRSHGFENNNEVFNYYHSSLRCTIERTFGVWKNRFAILRRMPKFTIKTQVEVVVATMAIHNFIRRNADMDVDFNRYEDEDITLDHDDYRRPVNLDSSQNLNIASSSEMNHVRNSVRDQIIEFKKNH*