AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0136800

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0136800 MtrunA17_Chr1g0208961 84.543 317 29 4 28 337 12 315 2.17e-156 471
Msa0136800 MtrunA17_Chr8g0392581 45.122 164 81 3 178 337 101 259 8.01e-34 133
Msa0136800 MtrunA17_Chr8g0355291 32.381 210 102 6 146 337 48 235 1.39e-22 99.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0136800 AT2G01130.1 37.903 248 96 9 97 336 8 205 6.72e-34 134
Msa0136800 AT2G01130.2 45.513 156 61 5 189 336 1 140 2.01e-25 108
Msa0136800 AT1G48650.1 36.923 195 97 5 152 335 104 283 5.58e-25 107
Msa0136800 AT1G48650.2 36.923 195 97 5 152 335 104 283 7.05e-25 107
Msa0136800 AT5G04895.1 34.132 167 89 5 179 337 93 246 1.22e-19 91.7

Find 100 sgRNAs with CRISPR-Local

Find 189 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAATCTCAACCATTGAATT+TGG 0.180668 1_3:-68991342 Msa0136800:CDS
GATGATGAAATGAGAGGTTT+AGG 0.249796 1_3:+68993355 None:intergenic
TTTCTCCACAACAGACTTAC+TGG 0.250673 1_3:+68991801 None:intergenic
CAAATTCAATGGTTGAGATT+TGG 0.252376 1_3:+68991343 None:intergenic
TCCGAATTACAGATATGATT+TGG 0.304280 1_3:-68992222 Msa0136800:CDS
TTACTGGATGCTAGTGGTTC+AGG 0.313607 1_3:+68991817 None:intergenic
TAGGTGGCGGTGGTTGATTT+TGG 0.321890 1_3:+68993205 None:intergenic
TTGGCTGAGTTTGACGGAAA+TGG 0.329951 1_3:+68993224 None:intergenic
GTTTACAGATGCAATGGATC+TGG 0.343553 1_3:-68991280 Msa0136800:CDS
CAACAACCTTCGAATACTTA+TGG 0.354503 1_3:+68992264 None:intergenic
TTTGCATTGTTGTTGGATAA+GGG 0.358184 1_3:+68993168 None:intergenic
ATTTGCATTGTTGTTGGATA+AGG 0.358840 1_3:+68993167 None:intergenic
TCCAAATCATATCTGTAATT+CGG 0.376353 1_3:+68992221 None:intergenic
GCGAGAATTTGCATTGTTGT+TGG 0.385626 1_3:+68993161 None:intergenic
CAGCGAAGGGCGGTCCTTCA+TGG 0.385627 1_3:+68993329 None:intergenic
CTTGATCCAATGCCGAGATT+TGG 0.385973 1_3:-68991312 Msa0136800:CDS
TTTGTGTTCAAACTCGTTGC+TGG 0.386449 1_3:+68994075 None:intergenic
ATGATGAAATGAGAGGTTTA+GGG 0.388661 1_3:+68993356 None:intergenic
TGGAGAGTGGTAGGTGGCGG+TGG 0.392491 1_3:+68993195 None:intergenic
CAATGCAAGGTTCGGTAAAA+CGG 0.397118 1_3:+68993425 None:intergenic
TGGCTGAGTTTGACGGAAAT+GGG 0.409880 1_3:+68993225 None:intergenic
GAAGGTTGTTGTCTTTAGTA+AGG 0.423332 1_3:-68992252 Msa0136800:CDS
GTTTGGAGAGTGGTAGGTGG+CGG 0.427082 1_3:+68993192 None:intergenic
AGCATCTGAAAGTTCGCTAC+AGG 0.440916 1_3:+68994114 None:intergenic
TGCATTGTTGTTGGATAAGG+GGG 0.441221 1_3:+68993170 None:intergenic
ATTGGAGCAATCCTCGGAAA+TGG 0.446317 1_3:+68993116 None:intergenic
TCGGTAAAACGGCGTCGTGC+TGG 0.456826 1_3:+68993436 None:intergenic
TGCACCAACCCGCTTGAATA+CGG 0.457905 1_3:+68991957 None:intergenic
CAACTAGAATGGGACTGTAT+AGG 0.464743 1_3:-68992389 Msa0136800:CDS
GGGGTTTGGAGAGTGGTAGG+TGG 0.468957 1_3:+68993189 None:intergenic
GGCAGTCTTGGTACAGATGA+AGG 0.469058 1_3:-68991853 Msa0136800:CDS
CGCCCTTCGCTGTCCTCCCA+TGG 0.471220 1_3:-68993318 Msa0136800:CDS
TGAGATTTGGATGAAAAGTC+TGG 0.477683 1_3:+68991356 None:intergenic
AGGAACGTGAAGTTGATGAT+TGG 0.484041 1_3:+68993098 None:intergenic
AGTTTGACGGAAATGGGAGC+GGG 0.484538 1_3:+68993231 None:intergenic
ATTCATTCATGTTCTCATTG+AGG 0.486248 1_3:+68992513 None:intergenic
TTTACAGATGCAATGGATCT+GGG 0.489380 1_3:-68991279 Msa0136800:CDS
TCAAGCAGTGATGGCAGTCT+TGG 0.491979 1_3:-68991865 Msa0136800:CDS
GAGTTTGACGGAAATGGGAG+CGG 0.493357 1_3:+68993230 None:intergenic
TCAATGAGAACATGAATGAA+TGG 0.499349 1_3:-68992511 Msa0136800:CDS
ATGCAACCAAATCTCGGCAT+TGG 0.500188 1_3:+68991306 None:intergenic
GCATTTGTAAGCTTATCTGC+CGG 0.501770 1_3:+68991773 None:intergenic
TTGGGAAATGGTGATGATGA+TGG 0.503619 1_3:+68993384 None:intergenic
CATTTAGTTTACAGATGCAA+TGG 0.506442 1_3:-68991286 Msa0136800:CDS
AATACCTTAGCCAAAAGTCC+AGG 0.511826 1_3:-68991924 Msa0136800:CDS
AAGATACAGTCCAAATTCAA+TGG 0.512274 1_3:+68991332 None:intergenic
ATGCAATGCAATGCAAGGTT+CGG 0.512399 1_3:+68993417 None:intergenic
AACTAAATGCAACCAAATCT+CGG 0.518910 1_3:+68991300 None:intergenic
CGCCATGGGAGGACAGCGAA+GGG 0.520336 1_3:+68993316 None:intergenic
AGAACATGAATGAATGGAAG+AGG 0.521095 1_3:-68992505 Msa0136800:CDS
TGATCAACAACAAGCCTGGC+AGG 0.525736 1_3:-68991749 Msa0136800:intron
TGCGTGATCAACAACAAGCC+TGG 0.534615 1_3:-68991753 Msa0136800:CDS
ATAAGTCAAAATAACCTGCC+AGG 0.535224 1_3:+68991735 None:intergenic
ATTTCATCATCTCGCCATGA+AGG 0.540138 1_3:-68993343 Msa0136800:CDS
TTGCATTGTTGTTGGATAAG+GGG 0.549617 1_3:+68993169 None:intergenic
AATGGTGATGATGATGGAGT+TGG 0.552564 1_3:+68993390 None:intergenic
CATTTGTAAGCTTATCTGCC+GGG 0.555167 1_3:+68991774 None:intergenic
GCCTCTCACCGTATTCAAGC+GGG 0.557077 1_3:-68991965 Msa0136800:CDS
TCATATCTGTAATTCGGAAG+TGG 0.560496 1_3:+68992227 None:intergenic
ACTTCACGTTCCTTCCCTAC+CGG 0.560609 1_3:-68993088 Msa0136800:intron
TTTGCAAGATCAAGCAGTGA+TGG 0.561713 1_3:-68991874 Msa0136800:CDS
GCGCCATGGGAGGACAGCGA+AGG 0.562050 1_3:+68993315 None:intergenic
GTAAGCAAGAGGTGATTTCG+AGG 0.562201 1_3:-68992454 Msa0136800:CDS
TTTAAATGAAGTTACCGGTA+GGG 0.567148 1_3:+68993074 None:intergenic
AGGTAACGACGGAGCGGAGA+CGG 0.570624 1_3:+68993271 None:intergenic
AGGAACGTAGATGGCGCCAT+GGG 0.573825 1_3:+68993302 None:intergenic
AGCATCCAGTAAGTCTGTTG+TGG 0.574492 1_3:-68991806 Msa0136800:CDS
AATGAAGTTACCGGTAGGGA+AGG 0.576255 1_3:+68993078 None:intergenic
TGTTCAAACTCGTTGCTGGA+AGG 0.577688 1_3:+68994079 None:intergenic
GAATGATAAGAGTAAGCAAG+AGG 0.578822 1_3:-68992465 Msa0136800:CDS
GAGCGGAGACGGTGGTGAGG+AGG 0.581688 1_3:+68993282 None:intergenic
CGAAAGACGGCCTCAGAGAG+AGG 0.581724 1_3:-68992198 Msa0136800:intron
TGGCAGCCATAAGTATTCGA+AGG 0.582788 1_3:-68992270 Msa0136800:intron
GATATGATTTGGACGAAAGA+CGG 0.583487 1_3:-68992211 Msa0136800:CDS
TTGATGATTGGAGCAATCCT+CGG 0.587443 1_3:+68993110 None:intergenic
GTGGTGAGGAGGAACGTAGA+TGG 0.589128 1_3:+68993293 None:intergenic
CTGTTGTGGAGAAAATTGCC+CGG 0.593445 1_3:-68991792 Msa0136800:CDS
AACGTAGATGGCGCCATGGG+AGG 0.598039 1_3:+68993305 None:intergenic
GAGGAACGTAGATGGCGCCA+TGG 0.598697 1_3:+68993301 None:intergenic
GCAGTCTTGGTACAGATGAA+GGG 0.604863 1_3:-68991852 Msa0136800:CDS
GTTTGACGGAAATGGGAGCG+GGG 0.610236 1_3:+68993232 None:intergenic
TAAGCAAGAGGTGATTTCGA+GGG 0.613142 1_3:-68992453 Msa0136800:CDS
TGCCTCTCACCGTATTCAAG+CGG 0.620064 1_3:-68991966 Msa0136800:CDS
ACGGAGCGGAGACGGTGGTG+AGG 0.621277 1_3:+68993279 None:intergenic
TGGCGAGATGATGAAATGAG+AGG 0.626093 1_3:+68993349 None:intergenic
GCATCTGAAAGTTCGCTACA+GGG 0.627072 1_3:+68994115 None:intergenic
GAAGCATGCAATGCAATGCA+AGG 0.630140 1_3:+68993412 None:intergenic
GTATCTGTGTGTCAGTGTGA+CGG 0.634018 1_3:+68993493 None:intergenic
ACCCGCTTGAATACGGTGAG+AGG 0.634900 1_3:+68991964 None:intergenic
AGTAGCATCTGAATTAGCCA+TGG 0.639015 1_3:+68994147 None:intergenic
ATACCTTAGCCAAAAGTCCA+GGG 0.641918 1_3:-68991923 Msa0136800:CDS
CTCACCGTATTCAAGCGGGT+TGG 0.642411 1_3:-68991961 Msa0136800:CDS
CATGGGAGGACAGCGAAGGG+CGG 0.644713 1_3:+68993319 None:intergenic
TTACGACGATGCCATTTCCG+AGG 0.647450 1_3:-68993127 Msa0136800:CDS
AGCGGCGGCGGAGGTAACGA+CGG 0.650245 1_3:+68993260 None:intergenic
TTTGACGGAAATGGGAGCGG+GGG 0.653466 1_3:+68993233 None:intergenic
TAACGACGGAGCGGAGACGG+TGG 0.663720 1_3:+68993274 None:intergenic
CGGCGGAGGTAACGACGGAG+CGG 0.665360 1_3:+68993265 None:intergenic
ATGACATATACCTCTCTCTG+AGG 0.684269 1_3:+68992188 None:intergenic
ACAGACTTACTGGATGCTAG+TGG 0.684761 1_3:+68991811 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATTTTTTATAGAAAGAAT+AGG + chr1_3:68991394-68991413 None:intergenic 10.0%
!! ATATATATAAAATTAAGTGT+CGG - chr1_3:68992652-68992671 Msa0136800:intron 10.0%
!! GCTTAAATATAACATAATTA+AGG - chr1_3:68991494-68991513 Msa0136800:intron 15.0%
!! AACTATAAATTTAAATAAGG+TGG + chr1_3:68991645-68991664 None:intergenic 15.0%
!! CCTAACTATAAATTTAAATA+AGG + chr1_3:68991648-68991667 None:intergenic 15.0%
!!! CCTTATTTAAATTTATAGTT+AGG - chr1_3:68991645-68991664 Msa0136800:intron 15.0%
!! ATTTAAATTTATAGTTAGGA+TGG - chr1_3:68991649-68991668 Msa0136800:intron 15.0%
!! AACTATAAATTCAAATAAAG+TGG + chr1_3:68991680-68991699 None:intergenic 15.0%
!! ATTTGAATTTATAGTTAGAA+TGG - chr1_3:68991684-68991703 Msa0136800:intron 15.0%
!!! ATTTTTTATCAAAATTGTGT+TGG + chr1_3:68991725-68991744 None:intergenic 15.0%
!! CATGTAAATATTTGAAAAAA+TGG - chr1_3:68992706-68992725 Msa0136800:intron 15.0%
!!! TATCTAAAATCATAGAAAAT+GGG + chr1_3:68993281-68993300 None:intergenic 15.0%
!! TAAAAAATATACAATCTCTC+AGG - chr1_3:68991404-68991423 Msa0136800:intron 20.0%
!!! ATTTTCAATAAATCATGTCT+CGG + chr1_3:68991451-68991470 None:intergenic 20.0%
!!! GAGATTTTTTTTGTCTATAT+CGG - chr1_3:68991579-68991598 Msa0136800:intron 20.0%
!! AGAAATTTAAATGAAGTTAC+CGG + chr1_3:68992353-68992372 None:intergenic 20.0%
!! AAAGATAAATAAACATACGA+AGG + chr1_3:68992376-68992395 None:intergenic 20.0%
!! ATTATTTACACATGAATAGA+AGG + chr1_3:68992627-68992646 None:intergenic 20.0%
!!! AAACAATACTTTTGATTCAA+AGG + chr1_3:68993089-68993108 None:intergenic 20.0%
!!! TTTGAATCAAAAGTATTGTT+TGG - chr1_3:68993088-68993107 Msa0136800:intron 20.0%
!!! GTATCTAAAATCATAGAAAA+TGG + chr1_3:68993282-68993301 None:intergenic 20.0%
!!! AATAACTAATAGCTTTACTA+AGG + chr1_3:68993421-68993440 None:intergenic 20.0%
!! TAAAGCTATTAGTTATTGAT+AGG - chr1_3:68993424-68993443 Msa0136800:CDS 20.0%
! ATCTCTTCTATTATCGTTTA+TGG + chr1_3:68991564-68991583 None:intergenic 25.0%
!!! TTTATGTGTTGTTTTTTGCT+TGG - chr1_3:68992855-68992874 Msa0136800:intron 25.0%
!!! TGATTTTTTGTTTTGTAGAG+TGG - chr1_3:68992880-68992899 Msa0136800:intron 25.0%
!!! ATCAAAAGTATTGTTTGGTT+TGG - chr1_3:68993093-68993112 Msa0136800:CDS 25.0%
! AATTTGATTCTGCTTGTTTA+TGG - chr1_3:68993129-68993148 Msa0136800:CDS 25.0%
! TCCAAATCATATCTGTAATT+CGG + chr1_3:68993201-68993220 None:intergenic 25.0%
!!! AATTTGAAACAAGTTTTCCT+CGG - chr1_3:68993907-68993926 Msa0136800:intron 25.0%
! TATGCAATATGAAACTATAC+TGG - chr1_3:68993963-68993982 Msa0136800:intron 25.0%
CTTCTATTATCGTTTATGGA+GGG + chr1_3:68991560-68991579 None:intergenic 30.0%
TCTTCTATTATCGTTTATGG+AGG + chr1_3:68991561-68991580 None:intergenic 30.0%
! GTGATCTTCTTAAGCTTTTA+AGG + chr1_3:68991752-68991771 None:intergenic 30.0%
!!! TTTAGGGTTTTTTTGGGAAA+TGG + chr1_3:68992050-68992069 None:intergenic 30.0%
ATGATGAAATGAGAGGTTTA+GGG + chr1_3:68992066-68992085 None:intergenic 30.0%
! TTTGCATTGTTGTTGGATAA+GGG + chr1_3:68992254-68992273 None:intergenic 30.0%
! ATTTGCATTGTTGTTGGATA+AGG + chr1_3:68992255-68992274 None:intergenic 30.0%
TTTAAATGAAGTTACCGGTA+GGG + chr1_3:68992348-68992367 None:intergenic 30.0%
ATTTAAATGAAGTTACCGGT+AGG + chr1_3:68992349-68992368 None:intergenic 30.0%
TCATCTCTTCAACTTATTAC+GGG + chr1_3:68992471-68992490 None:intergenic 30.0%
GTCATCTCTTCAACTTATTA+CGG + chr1_3:68992472-68992491 None:intergenic 30.0%
ATTCATTCATGTTCTCATTG+AGG + chr1_3:68992909-68992928 None:intergenic 30.0%
TCAATGAGAACATGAATGAA+TGG - chr1_3:68992908-68992927 Msa0136800:intron 30.0%
!! TTTTGATGACATTGCAGTTT+TGG - chr1_3:68993007-68993026 Msa0136800:intron 30.0%
TCCGAATTACAGATATGATT+TGG - chr1_3:68993197-68993216 Msa0136800:CDS 30.0%
ATAGAAAATGGGATAGTAGA+TGG + chr1_3:68993270-68993289 None:intergenic 30.0%
TAGATACTATACAAAAGCTC+AGG - chr1_3:68993295-68993314 Msa0136800:CDS 30.0%
AGATACTATACAAAAGCTCA+GGG - chr1_3:68993296-68993315 Msa0136800:CDS 30.0%
AAGCAGAAGAATAGTAGAAA+GGG + chr1_3:68993351-68993370 None:intergenic 30.0%
CAAATTCAATGGTTGAGATT+TGG + chr1_3:68994079-68994098 None:intergenic 30.0%
CAAATCTCAACCATTGAATT+TGG - chr1_3:68994077-68994096 Msa0136800:intron 30.0%
AAGATACAGTCCAAATTCAA+TGG + chr1_3:68994090-68994109 None:intergenic 30.0%
AACTAAATGCAACCAAATCT+CGG + chr1_3:68994122-68994141 None:intergenic 30.0%
! CATTTAGTTTACAGATGCAA+TGG - chr1_3:68994133-68994152 Msa0136800:CDS 30.0%
ATTTAAGCTTGATGCAGAAG+TGG + chr1_3:68991482-68991501 None:intergenic 35.0%
ACATCTTAGGTCATTTCACT+CGG + chr1_3:68991826-68991845 None:intergenic 35.0%
!!! GAGAGGTTTAGGGTTTTTTT+GGG + chr1_3:68992056-68992075 None:intergenic 35.0%
!!! TGAGAGGTTTAGGGTTTTTT+TGG + chr1_3:68992057-68992076 None:intergenic 35.0%
GATGATGAAATGAGAGGTTT+AGG + chr1_3:68992067-68992086 None:intergenic 35.0%
!!! TTGATTTTGGCTGAGTTTGA+CGG + chr1_3:68992204-68992223 None:intergenic 35.0%
! TTGCATTGTTGTTGGATAAG+GGG + chr1_3:68992253-68992272 None:intergenic 35.0%
ATATGTGTAAGTGTAGTCTC+AGG + chr1_3:68992443-68992462 None:intergenic 35.0%
TAACGACATGTATCTTGTGA+CGG - chr1_3:68992513-68992532 Msa0136800:CDS 35.0%
! ATACCTTCTATCAGAAGTGT+CGG - chr1_3:68992769-68992788 Msa0136800:intron 35.0%
TGTAATTCAAAGTGTAACCG+TGG - chr1_3:68992794-68992813 Msa0136800:intron 35.0%
GTAATTCAAAGTGTAACCGT+GGG - chr1_3:68992795-68992814 Msa0136800:intron 35.0%
AGAACATGAATGAATGGAAG+AGG - chr1_3:68992914-68992933 Msa0136800:intron 35.0%
GAATGATAAGAGTAAGCAAG+AGG - chr1_3:68992954-68992973 Msa0136800:intron 35.0%
ATTTCGAGGGAGAAAAAGAT+AGG - chr1_3:68992979-68992998 Msa0136800:intron 35.0%
CAACAACCTTCGAATACTTA+TGG + chr1_3:68993158-68993177 None:intergenic 35.0%
GAAGGTTGTTGTCTTTAGTA+AGG - chr1_3:68993167-68993186 Msa0136800:CDS 35.0%
TCATATCTGTAATTCGGAAG+TGG + chr1_3:68993195-68993214 None:intergenic 35.0%
! GATATGATTTGGACGAAAGA+CGG - chr1_3:68993208-68993227 Msa0136800:CDS 35.0%
AGCAGAAGAATAGTAGAAAG+GGG + chr1_3:68993350-68993369 None:intergenic 35.0%
CAAGCAGAAGAATAGTAGAA+AGG + chr1_3:68993352-68993371 None:intergenic 35.0%
! TTTTCTTTACCCTGGACTTT+TGG + chr1_3:68993508-68993527 None:intergenic 35.0%
! TGAAAAGCTTTTCTTTACCC+TGG + chr1_3:68993516-68993535 None:intergenic 35.0%
ATAAGTCAAAATAACCTGCC+AGG + chr1_3:68993687-68993706 None:intergenic 35.0%
AACGTTAACAATGCAGAACA+AGG + chr1_3:68993733-68993752 None:intergenic 35.0%
ACTAAACTACTAAAGAACCG+AGG + chr1_3:68993927-68993946 None:intergenic 35.0%
TGAGATTTGGATGAAAAGTC+TGG + chr1_3:68994066-68994085 None:intergenic 35.0%
TTTACAGATGCAATGGATCT+GGG - chr1_3:68994140-68994159 Msa0136800:CDS 35.0%
TTTGTGTTCAAACTCGTTGC+TGG + chr1_3:68991347-68991366 None:intergenic 40.0%
CTATTATCGTTTATGGAGGG+TGG + chr1_3:68991557-68991576 None:intergenic 40.0%
AAAGCGTGCAGTTACATCTT+AGG + chr1_3:68991839-68991858 None:intergenic 40.0%
! CAATGCAAGGTTCGGTAAAA+CGG + chr1_3:68991997-68992016 None:intergenic 40.0%
ATGCAATGCAATGCAAGGTT+CGG + chr1_3:68992005-68992024 None:intergenic 40.0%
!! AATGGTGATGATGATGGAGT+TGG + chr1_3:68992032-68992051 None:intergenic 40.0%
! TTGGGAAATGGTGATGATGA+TGG + chr1_3:68992038-68992057 None:intergenic 40.0%
ATTTCATCATCTCGCCATGA+AGG - chr1_3:68992076-68992095 Msa0136800:intron 40.0%
! TGCATTGTTGTTGGATAAGG+GGG + chr1_3:68992252-68992271 None:intergenic 40.0%
GCGAGAATTTGCATTGTTGT+TGG + chr1_3:68992261-68992280 None:intergenic 40.0%
! TTGATGATTGGAGCAATCCT+CGG + chr1_3:68992312-68992331 None:intergenic 40.0%
!! AGGAACGTGAAGTTGATGAT+TGG + chr1_3:68992324-68992343 None:intergenic 40.0%
!! CTACTTAGTACTGATACCTC+TGG + chr1_3:68992572-68992591 None:intergenic 40.0%
CCACTACTATACAAAACCCA+CGG + chr1_3:68992814-68992833 None:intergenic 40.0%
TAAGCAAGAGGTGATTTCGA+GGG - chr1_3:68992966-68992985 Msa0136800:intron 40.0%
!! TGCAGTTTTGGCAACTAGAA+TGG - chr1_3:68993019-68993038 Msa0136800:intron 40.0%
!! GCAGTTTTGGCAACTAGAAT+GGG - chr1_3:68993020-68993039 Msa0136800:intron 40.0%
CAACTAGAATGGGACTGTAT+AGG - chr1_3:68993030-68993049 Msa0136800:intron 40.0%
ATGACATATACCTCTCTCTG+AGG + chr1_3:68993234-68993253 None:intergenic 40.0%
CAGAAGAATAGTAGAAAGGG+GGG + chr1_3:68993348-68993367 None:intergenic 40.0%
GCAGAAGAATAGTAGAAAGG+GGG + chr1_3:68993349-68993368 None:intergenic 40.0%
AATACCTTAGCCAAAAGTCC+AGG - chr1_3:68993495-68993514 Msa0136800:CDS 40.0%
ATACCTTAGCCAAAAGTCCA+GGG - chr1_3:68993496-68993515 Msa0136800:CDS 40.0%
TTTGCAAGATCAAGCAGTGA+TGG - chr1_3:68993545-68993564 Msa0136800:intron 40.0%
TTTCTCCACAACAGACTTAC+TGG + chr1_3:68993621-68993640 None:intergenic 40.0%
CATTTGTAAGCTTATCTGCC+GGG + chr1_3:68993648-68993667 None:intergenic 40.0%
GCATTTGTAAGCTTATCTGC+CGG + chr1_3:68993649-68993668 None:intergenic 40.0%
GTTTACAGATGCAATGGATC+TGG - chr1_3:68994139-68994158 Msa0136800:CDS 40.0%
GCATCTGAAAGTTCGCTACA+GGG + chr1_3:68991307-68991326 None:intergenic 45.0%
AGCATCTGAAAGTTCGCTAC+AGG + chr1_3:68991308-68991327 None:intergenic 45.0%
TGTTCAAACTCGTTGCTGGA+AGG + chr1_3:68991343-68991362 None:intergenic 45.0%
AGGTCATTTCACTCGGTCAA+CGG + chr1_3:68991819-68991838 None:intergenic 45.0%
GTATCTGTGTGTCAGTGTGA+CGG + chr1_3:68991929-68991948 None:intergenic 45.0%
GAAGCATGCAATGCAATGCA+AGG + chr1_3:68992010-68992029 None:intergenic 45.0%
TGGCGAGATGATGAAATGAG+AGG + chr1_3:68992073-68992092 None:intergenic 45.0%
TGGCTGAGTTTGACGGAAAT+GGG + chr1_3:68992197-68992216 None:intergenic 45.0%
TTGGCTGAGTTTGACGGAAA+TGG + chr1_3:68992198-68992217 None:intergenic 45.0%
TGTTGTTGGATAAGGGGGTT+TGG + chr1_3:68992247-68992266 None:intergenic 45.0%
ATTGGAGCAATCCTCGGAAA+TGG + chr1_3:68992306-68992325 None:intergenic 45.0%
AATGAAGTTACCGGTAGGGA+AGG + chr1_3:68992344-68992363 None:intergenic 45.0%
CATGTATCTTGTGACGGTGT+TGG - chr1_3:68992519-68992538 Msa0136800:CDS 45.0%
AGACCGACACTTCTGATAGA+AGG + chr1_3:68992775-68992794 None:intergenic 45.0%
!! CCGTGGGTTTTGTATAGTAG+TGG - chr1_3:68992811-68992830 Msa0136800:intron 45.0%
GTAAGCAAGAGGTGATTTCG+AGG - chr1_3:68992965-68992984 Msa0136800:intron 45.0%
TGGCAGCCATAAGTATTCGA+AGG - chr1_3:68993149-68993168 Msa0136800:CDS 45.0%
GTAGAAAGGGGGGAGTAAAA+AGG + chr1_3:68993338-68993357 None:intergenic 45.0%
!! TTACCCTGGACTTTTGGCTA+AGG + chr1_3:68993502-68993521 None:intergenic 45.0%
GCAGTCTTGGTACAGATGAA+GGG - chr1_3:68993567-68993586 Msa0136800:intron 45.0%
TTACTGGATGCTAGTGGTTC+AGG + chr1_3:68993605-68993624 None:intergenic 45.0%
ACAGACTTACTGGATGCTAG+TGG + chr1_3:68993611-68993630 None:intergenic 45.0%
AGCATCCAGTAAGTCTGTTG+TGG - chr1_3:68993613-68993632 Msa0136800:intron 45.0%
CTGTTGTGGAGAAAATTGCC+CGG - chr1_3:68993627-68993646 Msa0136800:intron 45.0%
CTTGATCCAATGCCGAGATT+TGG - chr1_3:68994107-68994126 Msa0136800:CDS 45.0%
ATGCAACCAAATCTCGGCAT+TGG + chr1_3:68994116-68994135 None:intergenic 45.0%
TTTATGGAGGGTGGAGCTGA+TGG + chr1_3:68991548-68991567 None:intergenic 50.0%
AGTTTGACGGAAATGGGAGC+GGG + chr1_3:68992191-68992210 None:intergenic 50.0%
GAGTTTGACGGAAATGGGAG+CGG + chr1_3:68992192-68992211 None:intergenic 50.0%
!! TAGGTGGCGGTGGTTGATTT+TGG + chr1_3:68992217-68992236 None:intergenic 50.0%
! TTACGACGATGCCATTTCCG+AGG - chr1_3:68992292-68992311 Msa0136800:intron 50.0%
ACTTCACGTTCCTTCCCTAC+CGG - chr1_3:68992331-68992350 Msa0136800:intron 50.0%
GTAGTCTCAGGTGTCTGTGT+CGG + chr1_3:68992431-68992450 None:intergenic 50.0%
TGCCTCTCACCGTATTCAAG+CGG - chr1_3:68993453-68993472 Msa0136800:CDS 50.0%
TGCACCAACCCGCTTGAATA+CGG + chr1_3:68993465-68993484 None:intergenic 50.0%
!!! GCGGGTTGGTGCATATTTTG+AGG - chr1_3:68993472-68993491 Msa0136800:CDS 50.0%
TCAAGCAGTGATGGCAGTCT+TGG - chr1_3:68993554-68993573 Msa0136800:intron 50.0%
GGCAGTCTTGGTACAGATGA+AGG - chr1_3:68993566-68993585 Msa0136800:intron 50.0%
TGCGTGATCAACAACAAGCC+TGG - chr1_3:68993666-68993685 Msa0136800:intron 50.0%
TGATCAACAACAAGCCTGGC+AGG - chr1_3:68993670-68993689 Msa0136800:intron 50.0%
AGGAACGTAGATGGCGCCAT+GGG + chr1_3:68992120-68992139 None:intergenic 55.0%
GTGGTGAGGAGGAACGTAGA+TGG + chr1_3:68992129-68992148 None:intergenic 55.0%
TTTGACGGAAATGGGAGCGG+GGG + chr1_3:68992189-68992208 None:intergenic 55.0%
GTTTGACGGAAATGGGAGCG+GGG + chr1_3:68992190-68992209 None:intergenic 55.0%
GTTTGGAGAGTGGTAGGTGG+CGG + chr1_3:68992230-68992249 None:intergenic 55.0%
AAGGGGGTTTGGAGAGTGGT+AGG + chr1_3:68992236-68992255 None:intergenic 55.0%
GGATAAGGGGGTTTGGAGAG+TGG + chr1_3:68992240-68992259 None:intergenic 55.0%
! TGTGTCGGTGTGTCAGGTGT+CGG + chr1_3:68992416-68992435 None:intergenic 55.0%
! GGTGTTGGACACTGACACAC+CGG - chr1_3:68992534-68992553 Msa0136800:intron 55.0%
GTGTTGGACACTGACACACC+GGG - chr1_3:68992535-68992554 Msa0136800:intron 55.0%
GCCTCTCACCGTATTCAAGC+GGG - chr1_3:68993454-68993473 Msa0136800:CDS 55.0%
ACCCGCTTGAATACGGTGAG+AGG + chr1_3:68993458-68993477 None:intergenic 55.0%
CTCACCGTATTCAAGCGGGT+TGG - chr1_3:68993458-68993477 Msa0136800:CDS 55.0%
TCGGTAAAACGGCGTCGTGC+TGG + chr1_3:68991986-68992005 None:intergenic 60.0%
AACGTAGATGGCGCCATGGG+AGG + chr1_3:68992117-68992136 None:intergenic 60.0%
GAGGAACGTAGATGGCGCCA+TGG + chr1_3:68992121-68992140 None:intergenic 60.0%
AGGTAACGACGGAGCGGAGA+CGG + chr1_3:68992151-68992170 None:intergenic 60.0%
AATGGGAGCGGGGGAGTTAG+CGG + chr1_3:68992180-68992199 None:intergenic 60.0%
GGGGTTTGGAGAGTGGTAGG+TGG + chr1_3:68992233-68992252 None:intergenic 60.0%
!! GTGTCAGGTGTCGGTGCTTC+AGG + chr1_3:68992407-68992426 None:intergenic 60.0%
! GGTGTCTGTGTCGGTGTGTC+AGG + chr1_3:68992422-68992441 None:intergenic 60.0%
CCTCTGGTAGAAGCTGTGCC+CGG + chr1_3:68992556-68992575 None:intergenic 60.0%
CGAAAGACGGCCTCAGAGAG+AGG - chr1_3:68993221-68993240 Msa0136800:CDS 60.0%
CAGCGAAGGGCGGTCCTTCA+TGG + chr1_3:68992093-68992112 None:intergenic 65.0%
CATGGGAGGACAGCGAAGGG+CGG + chr1_3:68992103-68992122 None:intergenic 65.0%
CGCCATGGGAGGACAGCGAA+GGG + chr1_3:68992106-68992125 None:intergenic 65.0%
TAACGACGGAGCGGAGACGG+TGG + chr1_3:68992148-68992167 None:intergenic 65.0%
! TGGAGAGTGGTAGGTGGCGG+TGG + chr1_3:68992227-68992246 None:intergenic 65.0%
CCGGGCACAGCTTCTACCAG+AGG - chr1_3:68992553-68992572 Msa0136800:intron 65.0%
CGCCCTTCGCTGTCCTCCCA+TGG - chr1_3:68992101-68992120 Msa0136800:intron 70.0%
GCGCCATGGGAGGACAGCGA+AGG + chr1_3:68992107-68992126 None:intergenic 70.0%
GAGCGGAGACGGTGGTGAGG+AGG + chr1_3:68992140-68992159 None:intergenic 70.0%
ACGGAGCGGAGACGGTGGTG+AGG + chr1_3:68992143-68992162 None:intergenic 70.0%
CGGCGGAGGTAACGACGGAG+CGG + chr1_3:68992157-68992176 None:intergenic 70.0%
AGCGGCGGCGGAGGTAACGA+CGG + chr1_3:68992162-68992181 None:intergenic 70.0%
AGCGGGGGAGTTAGCGGCGG+CGG + chr1_3:68992174-68992193 None:intergenic 75.0%
GGGAGCGGGGGAGTTAGCGG+CGG + chr1_3:68992177-68992196 None:intergenic 75.0%
! GGGGGAGTTAGCGGCGGCGG+AGG + chr1_3:68992171-68992190 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 68991274 68994167 68991274 ID=Msa0136800;Name=Msa0136800
chr1_3 mRNA 68991274 68994167 68991274 ID=Msa0136800-mRNA-1;Parent=Msa0136800;Name=Msa0136800-mRNA-1;_AED=0.12;_eAED=0.12;_QI=0|0.4|0.16|0.66|1|1|6|0|376
chr1_3 exon 68994088 68994167 68994088 ID=Msa0136800-mRNA-1:exon:23835;Parent=Msa0136800-mRNA-1
chr1_3 exon 68993089 68993524 68993089 ID=Msa0136800-mRNA-1:exon:23834;Parent=Msa0136800-mRNA-1
chr1_3 exon 68992386 68992543 68992386 ID=Msa0136800-mRNA-1:exon:23833;Parent=Msa0136800-mRNA-1
chr1_3 exon 68992199 68992286 68992199 ID=Msa0136800-mRNA-1:exon:23832;Parent=Msa0136800-mRNA-1
chr1_3 exon 68991750 68991995 68991750 ID=Msa0136800-mRNA-1:exon:23831;Parent=Msa0136800-mRNA-1
chr1_3 exon 68991274 68991396 68991274 ID=Msa0136800-mRNA-1:exon:23830;Parent=Msa0136800-mRNA-1
chr1_3 CDS 68994088 68994167 68994088 ID=Msa0136800-mRNA-1:cds;Parent=Msa0136800-mRNA-1
chr1_3 CDS 68993089 68993524 68993089 ID=Msa0136800-mRNA-1:cds;Parent=Msa0136800-mRNA-1
chr1_3 CDS 68992386 68992543 68992386 ID=Msa0136800-mRNA-1:cds;Parent=Msa0136800-mRNA-1
chr1_3 CDS 68992199 68992286 68992199 ID=Msa0136800-mRNA-1:cds;Parent=Msa0136800-mRNA-1
chr1_3 CDS 68991750 68991995 68991750 ID=Msa0136800-mRNA-1:cds;Parent=Msa0136800-mRNA-1
chr1_3 CDS 68991274 68991396 68991274 ID=Msa0136800-mRNA-1:cds;Parent=Msa0136800-mRNA-1
Gene Sequence

>Msa0136800

ATGGCTAATTCAGATGCTACTATTTTCTTACCCTGTAGCGAACTTTCAGATGCTACTACTTTCTTACCTTCCAGCAACGATTCATTCTTCCGTCACACTGACACACAGATACACGCACACATAGAATCGACTCATCACAACAATATCCAGCACGACGCCGTTTTACCGAACCTTGCATTGCATTGCATGCTTCCAACTCCATCATCATCACCATTTCCCAAAAAAACCCTAAACCTCTCATTTCATCATCTCGCCATGAAGGACCGCCCTTCGCTGTCCTCCCATGGCGCCATCTACGTTCCTCCTCACCACCGTCTCCGCTCCGTCGTTACCTCCGCCGCCGCTAACTCCCCCGCTCCCATTTCCGTCAAACTCAGCCAAAATCAACCACCGCCACCTACCACTCTCCAAACCCCCTTATCCAACAACAATGCAAATTCTCGCTTCGTTTCTGCTTACGACGATGCCATTTCCGAGGATTGCTCCAATCATCAACTTCACGTTCCTTCCCTACCGAGTGGTTACCTCAATGAGAACATGAATGAATGGAAGAGGAAATTAACGATGCTGTTGAATGATAAGAGTAAGCAAGAGGTGATTTCGAGGGAGAAAAAGATAGGCGTGATTTTGATGACATTGCAGTTTTGGCAACTAGAATGGGACTGTATAGGTGTCCATAAGTATTCGAAGGTTGTTGTCTTTAGTAAGGCGCCACTTCCGAATTACAGATATGATTTGGACGAAAGACGGCCTCAGAGAGAGGTGAGCATGCCTCTCACCGTATTCAAGCGGGTTGGTGCATATTTTGAGGAATACCTTAGCCAAAAGTCCAGGGTAAAGAAAAGCTTTTCAGATTCGTCTTTTGCAAGATCAAGCAGTGATGGCAGTCTTGGTACAGATGAAGGGTTTTTTGTGCAACCTGAACCACTAGCATCCAGTAAGTCTGTTGTGGAGAAAATTGCCCGGCAGATAAGCTTACAAATGCGTGATCAACAACAAGCCTGGCAGAAACTCATGCTGCACTGTCCAGACTTTTCATCCAAATCTCAACCATTGAATTTGGACTGTATCTTGATCCAATGCCGAGATTTGGTTGCATTTAGTTTACAGATGCAATGGATCTGGGTTTGA
Protein sequence

>Msa0136800

MANSDATIFLPCSELSDATTFLPSSNDSFFRHTDTQIHAHIESTHHNNIQHDAVLPNLALHCMLPTPSSSPFPKKTLNLSFHHLAMKDRPSLSSHGAIYVPPHHRLRSVVTSAAANSPAPISVKLSQNQPPPPTTLQTPLSNNNANSRFVSAYDDAISEDCSNHQLHVPSLPSGYLNENMNEWKRKLTMLLNDKSKQEVISREKKIGVILMTLQFWQLEWDCIGVHKYSKVVVFSKAPLPNYRYDLDERRPQREVSMPLTVFKRVGAYFEEYLSQKSRVKKSFSDSSFARSSSDGSLGTDEGFFVQPEPLASSKSVVEKIARQISLQMRDQQQAWQKLMLHCPDFSSKSQPLNLDCILIQCRDLVAFSLQMQWIWV*