AlfalfaGEDB | Msa0142320

M. sativa cultivar zhongmu-4 / Msa0142320

KEGG: K11644
GO: -
PFAM: PF02671.24

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0142320	XP_024637812.1	92.359	602	46	0	1	602	1	602	0.0	1126

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0142320	sp\|O48686\|SNL3_ARATH	39.735	151	82	2	11	152	13	163	5.58e-22	104
Msa0142320	sp\|O48686\|SNL3_ARATH	32.919	161	99	2	81	235	6	163	2.13e-16	87.0
Msa0142320	sp\|O48686\|SNL3_ARATH	35.152	165	85	4	175	326	17	172	2.45e-12	73.9

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0142320	A0A396IK85	91.694	602	50	0	1	602	1	602	0.0	1117

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TFs/TRs:

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Gene ID	Type	Classification
Msa0142320	TR	Others

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0142320	Msa0746400	0.925411	1.904301e-90	-8.615850e-47
Msa0142320	Msa1224560	0.921444	3.549221e-88	-8.615850e-47
Msa0142320	Msa1443370	0.937557	2.854636e-98	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0142320	MtrunA17_Chr4g0075971	91.694	602	50	0	1	602	1	602	0.0	1117
Msa0142320	MtrunA17_Chr4g0075861	92.493	373	28	0	1	373	1	373	0.0	692
Msa0142320	MtrunA17_Chr3g0077801	68.588	347	105	2	256	599	4	349	8.75e-167	479
Msa0142320	MtrunA17_Chr3g0077901	65.156	353	121	1	251	601	19	371	1.06e-163	472
Msa0142320	MtrunA17_Chr3g0077851	67.164	335	108	1	269	601	1	335	6.18e-159	458
Msa0142320	MtrunA17_Chr6g0488101	52.889	450	185	9	175	602	11	455	1.79e-153	449
Msa0142320	MtrunA17_Chr6g0488271	53.571	420	175	8	198	602	1	415	1.66e-145	427
Msa0142320	MtrunA17_Chr3g0112311	61.732	358	122	5	254	600	2	355	2.78e-138	406
Msa0142320	MtrunA17_Chr7g0252101	52.928	444	181	12	167	602	9	432	4.21e-132	393
Msa0142320	MtrunA17_Chr7g0243331	60.286	350	133	4	255	601	3	349	3.03e-130	385

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0142320	AT1G24190.1	39.735	151	82	2	11	152	13	163	5.68e-23	104
Msa0142320	AT1G24190.1	32.919	161	99	2	81	235	6	163	2.17e-17	87.0
Msa0142320	AT1G24190.1	35.152	165	85	4	175	326	17	172	2.49e-13	73.9
Msa0142320	AT1G24190.2	39.735	151	82	2	11	152	13	163	6.13e-23	104
Msa0142320	AT1G24190.2	32.919	161	99	2	81	235	6	163	2.20e-17	87.0
Msa0142320	AT1G24190.2	35.152	165	85	4	175	326	17	172	2.90e-13	73.9
Msa0142320	AT1G24190.3	39.735	151	82	2	11	152	13	163	6.16e-23	104
Msa0142320	AT1G24190.3	32.919	161	99	2	81	235	6	163	1.96e-17	87.0
Msa0142320	AT1G24190.3	35.152	165	85	4	175	326	17	172	2.76e-13	73.9
Msa0142320	AT1G70060.2	38.750	160	88	2	3	152	5	164	9.31e-22	100

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Find 103 sgRNAs with CRISPR-Local

Find 312 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGACCAGTCCAACCAGT+AGG	0.594037	1_4:+1227063	None:intergenic
AAAATGCAAAGTGTATGTAG+GGG	0.684341	1_4:-1227143	Msa0142320:CDS
AAAATTGACTTTAAAGGTTG+GGG	0.437076	1_4:+1227684	None:intergenic
AAAGACCAGTCCAACCAGTA+GGG	0.607927	1_4:+1227064	None:intergenic
AACCATGAAGTATGTCAAGT+TGG	0.523678	1_4:-1227284	Msa0142320:CDS
AACGCAGATCAAAAGCTACA+TGG	0.594334	1_4:+1226984	None:intergenic
AAGGCATAGTAGTTTCCACC+CGG	0.674044	1_4:+1227229	None:intergenic
AAGGCTCAAGGAATTGATAC+GGG	0.604072	1_4:-1231187	Msa0142320:CDS
AATGTGAATCCGTATCTTTC+TGG	0.260796	1_4:+1231340	None:intergenic
AATTGATACGGGAGGTGTTG+CGG	0.560033	1_4:-1231176	Msa0142320:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGTTGTGTTTCAAGATAAGA+TGG	-	chr1_4:1227236-1227255	Msa0142320:CDS	30.0%
ATTGCGATGAAGCTATTTAA+AGG	-	chr1_4:1227333-1227352	Msa0142320:CDS	30.0%
TTGCGATGAAGCTATTTAAA+GGG	-	chr1_4:1227334-1227353	Msa0142320:CDS	30.0%
TTCCAACTTATCATGAAACA+CGG	+	chr1_4:1227490-1227509	None:intergenic	30.0%
ACAAGTTATCACAAATCACA+AGG	-	chr1_4:1227527-1227546	Msa0142320:CDS	30.0%
TTGGGAATGATCATATATCA+AGG	+	chr1_4:1227817-1227836	None:intergenic	30.0%
TAGAACCTAGTATTTGAGAA+AGG	+	chr1_4:1227884-1227903	None:intergenic	30.0%
TTTCTCAAATACTAGGTTCT+AGG	-	chr1_4:1227883-1227902	Msa0142320:CDS	30.0%
TTGTAGCAAGGAGAATAAAT+TGG	+	chr1_4:1228129-1228148	None:intergenic	30.0%
TCATTGAAAATAGGTTTAGG+TGG	+	chr1_4:1228154-1228173	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_4	gene	1226879	1231876	1226879	ID=Msa0142320;Name=Msa0142320
chr1_4	mRNA	1226879	1231876	1226879	ID=Msa0142320-mRNA-1;Parent=Msa0142320;Name=Msa0142320-mRNA-1;_AED=0.01;_eAED=0.01;_QI=64\|1\|1\|1\|0\|0\|2\|142\|602
chr1_4	exon	1226879	1228078	1226879	ID=Msa0142320-mRNA-1:exon:403;Parent=Msa0142320-mRNA-1
chr1_4	exon	1231062	1231876	1231062	ID=Msa0142320-mRNA-1:exon:402;Parent=Msa0142320-mRNA-1
chr1_4	five_prime_UTR	1231813	1231876	1231813	ID=Msa0142320-mRNA-1:five_prime_utr;Parent=Msa0142320-mRNA-1
chr1_4	CDS	1231062	1231812	1231062	ID=Msa0142320-mRNA-1:cds;Parent=Msa0142320-mRNA-1
chr1_4	CDS	1227021	1228078	1227021	ID=Msa0142320-mRNA-1:cds;Parent=Msa0142320-mRNA-1
chr1_4	three_prime_UTR	1226879	1227020	1226879	ID=Msa0142320-mRNA-1:three_prime_utr;Parent=Msa0142320-mRNA-1

Gene Sequence

>Msa0142320

ATGAATAGCTCCACAAGCGATGCTTTGTTTGATGATGCATGTGCGTATGACCATGCAGTAAAATCTACATGTCAAGAAAAGTATTCTGAATTTGCAGAAGTCATGAGTGATTACAGGGATGGACGAATTGATCCAAGAGGTGTCAAAGCAAGAGTGCATGAGTTGTTTAAAGGCCATAAACATTTAATTTTGGGAATCAACAACATCATGCCAAAGAATTATGAAATCATACTTCCTCCATCGGATGAAAAACTGAATCGACAAGATGCAACAACTTTTCTCAAACAAGTAAAAGTTGTGTTTCAAGATAAGATGGAAAAATATTATGAGTTTCTACAAGTCATACAAGACTACATGAATCTCACAATTGATATATTAGATGTCATCAAAATTGCGATGAAGCTATTTAAAGGGCATGTCGATTTACTTTCGGGATTCAACTACTTCTTGCCAGAAAGATACGGATTCACATTTCCACTGTTGCAAGATCATAAACAAGGTCATCAATTAGTGATAAAAGATGCATTTCTCAATGAAGTCAAAGCCGTGTTTCATGATAAGTTGGAAAAATATTTTGAGTTTTTACAAGTTATCACAAATCACAAGGCTCAAGGAATTGATACGGGAGGTGTTGCGGCAATAGTGAAAGAGTTATTTAAAGACCATACAAATTTAATTTTGGGATTCAATGCCTTGCTGCCAGAGGAACATCAAATCACACTTCCATTTGAGCTTCATTGCCATGAGACTGGGAAAAAAAGAATGGTAGCAGGGAACTATCAACTTTCTTGGGATGTCCTCGATATCATTTCCAAATCTCTAGACTTTGATGACCTCTTTCAATTTGCTTGTGTGTGTAAGAATTGGAGGGCATTTCATAAGATGTATTGGAGAGATTTCTTGGCATTACAAGAACCATTACTTGTTCAAAAGTCTTCCTTTTTCAAGAAATCTTTTTCCTTTATCAGCATACCTAACCAACAAGTTTATCGTTCAAAGATGATCAATTATTTCTGGCACTTTGCATATTCTGGATCTTCTAGTGGATATTTGATCATGACAGGGAACAATAATTCATTTTTGCTAATGAATCCATTTAGGAGAAGAAAGAAGGTAATCAATGCCCCAACCTTTAAAGTCAATTTTTCTTATTTTGCTTACCGTGTCTTGCTTGCTTTTGACAAAGGCTCCAAGGATTTTGTCTTAGTGGCTTTATGTAAAAGTTCTAATAGTTTGCATGTTTATCAATCTCGAAATTTTGGTTGGGTTACTTATTCAACAATGGGATACCCATGGATGATTGTTGACTTTGTTGTTTTGCATAACACTATATATGTTGTCAACGACAAGGGCAACATAGGGATACTCAACTTAAATTATGTGAATATAAAATTTTTAGAAATGAAGAGTATTCCTAGCGTAACTTCTTTGTCGCGCCTAAGGTTGGTTAGTTGTGACGAACAACTTTTTGTGGTTCATACCAAGTCTATGGTGGCATTTAATGTGTACAAGATTGACTTCTCAACCATGAAGTATGTCAAGTTGGAAACTTTGGGTGACATTGCATTATTATATGCTCCGGGTGGAAACTACTATGCCTTGAGCAACCCGAACATGTGGGGTTATGAAAGCAACTCTGTGTATGTTATCGATCATTCGTCTACAAAATGCAAAGTGTATGTAGGGGATGACAACAAGCTGCCAAAGTACATTAGGTCACATGATGATCTTCATACTACAAGACCCTACTGGTTGGACTGGTCTTTTAGACATTTTCACTACGAGATAGATTATTCTCTAGTTGACTAA

Protein sequence

>Msa0142320

MNSSTSDALFDDACAYDHAVKSTCQEKYSEFAEVMSDYRDGRIDPRGVKARVHELFKGHKHLILGINNIMPKNYEIILPPSDEKLNRQDATTFLKQVKVVFQDKMEKYYEFLQVIQDYMNLTIDILDVIKIAMKLFKGHVDLLSGFNYFLPERYGFTFPLLQDHKQGHQLVIKDAFLNEVKAVFHDKLEKYFEFLQVITNHKAQGIDTGGVAAIVKELFKDHTNLILGFNALLPEEHQITLPFELHCHETGKKRMVAGNYQLSWDVLDIISKSLDFDDLFQFACVCKNWRAFHKMYWRDFLALQEPLLVQKSSFFKKSFSFISIPNQQVYRSKMINYFWHFAYSGSSSGYLIMTGNNNSFLLMNPFRRRKKVINAPTFKVNFSYFAYRVLLAFDKGSKDFVLVALCKSSNSLHVYQSRNFGWVTYSTMGYPWMIVDFVVLHNTIYVVNDKGNIGILNLNYVNIKFLEMKSIPSVTSLSRLRLVSCDEQLFVVHTKSMVAFNVYKIDFSTMKYVKLETLGDIALLYAPGGNYYALSNPNMWGYESNSVYVIDHSSTKCKVYVGDDNKLPKYIRSHDDLHTTRPYWLDWSFRHFHYEIDYSLVD*