AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0150190

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0150190 MtrunA17_Chr6g0467251 70.103 194 27 3 1 167 1 190 3.45e-84 253
Msa0150190 MtrunA17_Chr6g0467251 92.941 85 6 0 138 222 264 348 4.72e-41 142
Msa0150190 MtrunA17_Chr6g0467121 46.552 174 66 2 1 147 33 206 1.70e-44 152
Msa0150190 MtrunA17_Chr6g0467121 54.762 84 37 1 136 218 297 380 2.24e-20 88.2
Msa0150190 MtrunA17_Chr6g0467161 43.956 182 75 2 9 164 15 195 2.18e-44 151
Msa0150190 MtrunA17_Chr6g0467161 56.716 67 29 0 138 204 272 338 1.22e-16 77.4
Msa0150190 MtrunA17_Chr5g0423911 81.429 70 13 0 138 207 7 76 6.43e-33 115
Msa0150190 MtrunA17_Chr3g0114451 33.117 154 72 2 11 138 3 151 6.07e-24 97.1
Msa0150190 MtrunA17_Chr4g0063671 42.149 121 48 1 39 137 21 141 1.00e-21 91.3
Msa0150190 MtrunA17_Chr5g0404721 36.885 122 51 1 43 138 34 155 1.12e-21 91.3
Msa0150190 MtrunA17_Chr4g0046131 34.839 155 70 3 12 137 17 169 3.66e-21 90.1
Msa0150190 MtrunA17_Chr2g0296621 31.868 182 90 4 39 198 50 219 3.84e-21 89.7
Msa0150190 MtrunA17_Chr3g0129051 28.634 227 112 6 1 199 1 205 6.08e-21 89.0
Msa0150190 MtrunA17_Chr5g0438021 31.373 153 72 2 11 137 10 155 9.36e-21 88.6
Msa0150190 MtrunA17_Chr4g0004591 44.554 101 43 1 39 139 77 164 2.17e-20 87.8
Msa0150190 MtrunA17_Chr4g0075241 34.694 147 65 4 22 137 7 153 2.91e-20 87.4
Msa0150190 MtrunA17_Chr2g0279041 29.630 216 113 5 11 204 4 202 4.72e-20 86.7
Msa0150190 MtrunA17_Chr4g0024121 29.139 151 75 3 13 138 8 151 4.87e-20 86.7
Msa0150190 MtrunA17_Chr6g0477071 32.000 125 60 1 38 137 53 177 5.48e-20 86.7
Msa0150190 MtrunA17_Chr8g0372601 36.066 122 52 1 43 138 34 155 1.07e-19 85.9
Msa0150190 MtrunA17_Chr5g0402571 29.018 224 111 5 3 200 1 202 1.43e-19 85.5
Msa0150190 MtrunA17_Chr2g0316791 39.370 127 50 3 39 138 76 202 2.13e-19 85.5
Msa0150190 MtrunA17_Chr2g0316481 38.583 127 51 3 39 138 76 202 3.01e-19 85.1
Msa0150190 MtrunA17_Chr4g0013821 30.435 184 91 4 43 200 32 204 3.49e-19 84.3
Msa0150190 MtrunA17_Chr7g0254231 36.000 125 55 2 39 138 22 146 3.54e-19 84.0
Msa0150190 MtrunA17_Chr5g0402581 38.931 131 54 3 33 137 21 151 3.58e-19 84.3
Msa0150190 MtrunA17_Chr2g0320091 33.607 122 55 1 42 137 28 149 5.71e-19 83.6
Msa0150190 MtrunA17_Chr4g0013721 30.435 184 91 3 43 200 104 276 5.82e-19 84.3
Msa0150190 MtrunA17_Chr4g0013201 35.374 147 64 3 34 152 41 184 6.26e-19 83.6
Msa0150190 MtrunA17_Chr2g0292151 37.857 140 55 3 39 152 29 162 6.52e-19 84.0
Msa0150190 MtrunA17_Chr2g0292151 45.000 80 40 2 142 217 268 347 2.50e-12 65.1
Msa0150190 MtrunA17_Chr2g0316191 38.583 127 51 3 39 138 76 202 6.94e-19 84.3
Msa0150190 MtrunA17_Chr2g0316831 38.583 127 51 3 39 138 112 238 7.64e-19 84.3
Msa0150190 MtrunA17_Chr1g0157051 32.215 149 71 2 15 138 5 148 8.13e-19 83.2
Msa0150190 MtrunA17_Chr2g0320031 26.108 203 106 3 13 189 7 191 1.07e-18 82.8
Msa0150190 MtrunA17_Chr2g0292211 32.192 146 67 3 34 153 35 174 1.14e-18 83.2
Msa0150190 MtrunA17_Chr2g0292211 52.542 59 26 1 142 198 279 337 1.37e-11 63.2
Msa0150190 MtrunA17_Chr1g0155611 34.921 126 56 1 38 137 45 170 1.15e-18 83.2
Msa0150190 MtrunA17_Chr2g0320101 29.348 184 89 5 42 195 28 200 1.15e-18 82.8
Msa0150190 MtrunA17_Chr2g0292241 31.373 153 79 2 39 165 29 181 1.37e-18 82.8
Msa0150190 MtrunA17_Chr5g0400331 30.729 192 96 4 39 204 26 206 1.50e-18 82.8
Msa0150190 MtrunA17_Chr2g0292181 33.333 141 62 3 39 153 38 172 1.76e-18 82.8
Msa0150190 MtrunA17_Chr8g0371381 35.772 123 54 1 39 136 26 148 1.83e-18 82.0
Msa0150190 MtrunA17_Chr4g0043391 27.586 232 123 5 10 213 2 216 2.29e-18 82.0
Msa0150190 MtrunA17_Chr2g0292291 30.928 194 93 4 39 204 30 210 2.35e-18 82.4
Msa0150190 MtrunA17_Chr2g0292291 50.000 60 28 1 141 198 269 328 6.95e-11 60.8
Msa0150190 MtrunA17_Chr2g0316811 38.889 126 50 3 39 137 50 175 2.57e-18 82.0
Msa0150190 MtrunA17_Chr4g0038171 28.054 221 116 4 19 213 10 213 2.61e-18 81.6
Msa0150190 MtrunA17_Chr2g0292111 35.461 141 59 3 39 153 29 163 2.61e-18 82.0
Msa0150190 MtrunA17_Chr2g0292111 46.377 69 35 1 142 208 268 336 2.49e-11 62.4
Msa0150190 MtrunA17_Chr1g0188431 27.179 195 105 3 39 208 39 221 2.85e-18 81.6
Msa0150190 MtrunA17_Chr1g0188431 48.276 58 30 0 141 198 266 323 9.18e-11 60.5
Msa0150190 MtrunA17_Chr2g0320071 29.348 184 89 5 42 195 28 200 3.52e-18 81.3
Msa0150190 MtrunA17_Chr1g0213061 29.032 155 76 2 13 141 9 155 3.80e-18 81.6
Msa0150190 MtrunA17_Chr2g0292261 34.109 129 59 1 39 141 29 157 4.76e-18 81.3
Msa0150190 MtrunA17_Chr2g0320061 26.437 174 91 2 42 189 28 190 5.05e-18 80.9
Msa0150190 MtrunA17_Chr2g0320051 27.011 174 90 2 42 189 28 190 8.84e-18 80.5
Msa0150190 MtrunA17_Chr4g0070951 33.594 128 61 1 39 142 28 155 9.70e-18 80.5
Msa0150190 MtrunA17_Chr6g0460711 30.370 135 69 2 33 142 15 149 1.04e-17 80.1
Msa0150190 MtrunA17_Chr5g0405991 34.400 125 57 1 37 136 21 145 1.27e-17 79.7
Msa0150190 MtrunA17_Chr7g0225221 33.065 124 57 1 42 139 28 151 1.44e-17 79.7
Msa0150190 MtrunA17_Chr1g0175561 28.916 166 78 3 3 141 1 153 1.46e-17 79.7
Msa0150190 MtrunA17_Chr7g0254201 32.061 131 64 2 33 138 14 144 1.47e-17 79.7
Msa0150190 MtrunA17_Chr4g0068621 29.648 199 101 5 39 209 38 225 1.75e-17 79.7
Msa0150190 MtrunA17_Chr1g0181431 28.906 128 66 2 35 137 23 150 3.38e-17 79.0
Msa0150190 MtrunA17_Chr3g0136751 30.921 152 74 3 9 138 4 146 3.52e-17 78.6
Msa0150190 MtrunA17_Chr4g0076211 29.508 183 95 3 44 204 46 216 3.94e-17 78.6
Msa0150190 MtrunA17_Chr3g0128061 32.800 125 58 1 39 137 26 150 4.68e-17 78.6
Msa0150190 MtrunA17_Chr7g0254171 38.144 97 49 1 102 198 46 131 4.97e-17 74.7
Msa0150190 MtrunA17_Chr4g0038161 35.200 125 55 2 39 137 24 148 6.05e-17 78.2
Msa0150190 MtrunA17_Chr2g0278671 30.818 159 77 4 39 173 24 173 6.15e-17 78.2
Msa0150190 MtrunA17_Chr2g0291751 38.462 130 52 4 39 141 29 157 7.70e-17 78.2
Msa0150190 MtrunA17_Chr2g0291751 46.377 69 35 1 142 208 268 336 1.17e-11 63.2
Msa0150190 MtrunA17_Chr1g0194271 29.299 157 79 3 8 136 3 155 7.99e-17 77.8
Msa0150190 MtrunA17_Chr2g0292271 32.558 129 61 1 39 141 29 157 1.65e-16 77.0
Msa0150190 MtrunA17_Chr2g0292271 47.368 76 38 1 141 214 267 342 2.36e-13 68.2
Msa0150190 MtrunA17_Chr7g0244461 30.159 126 62 1 39 138 20 145 1.86e-16 76.6
Msa0150190 MtrunA17_Chr2g0299691 29.717 212 110 6 17 204 38 234 1.91e-16 77.0
Msa0150190 MtrunA17_Chr2g0292091 34.043 141 61 3 39 153 24 158 2.01e-16 76.6
Msa0150190 MtrunA17_Chr7g0258811 27.907 215 106 6 13 198 50 244 2.32e-16 76.6
Msa0150190 MtrunA17_Chr4g0013171 29.193 161 78 3 4 137 1 152 3.43e-16 75.9
Msa0150190 MtrunA17_Chr4g0038151 32.800 125 58 1 39 137 25 149 3.57e-16 75.9
Msa0150190 MtrunA17_Chr2g0292251 32.558 129 61 2 39 141 28 156 6.08e-16 75.5
Msa0150190 MtrunA17_Chr4g0013191 35.246 122 52 2 43 137 49 170 1.95e-15 73.9
Msa0150190 MtrunA17_Chr3g0129041 31.902 163 74 3 1 137 1 152 2.41e-15 73.6
Msa0150190 MtrunA17_Chr8g0364291 33.065 124 56 2 42 138 26 149 2.52e-15 73.6
Msa0150190 MtrunA17_Chr1g0181481 26.613 124 66 2 39 137 27 150 3.00e-15 73.2
Msa0150190 MtrunA17_Chr5g0432441 31.098 164 76 5 1 137 1 154 4.71e-15 72.8
Msa0150190 MtrunA17_Chr5g0421411 38.542 96 33 1 69 138 11 106 4.85e-15 72.4
Msa0150190 MtrunA17_Chr2g0311241 38.679 106 41 3 39 120 60 165 5.34e-15 73.2
Msa0150190 MtrunA17_Chr7g0224371 31.405 121 57 2 43 137 35 155 7.38e-15 72.0
Msa0150190 MtrunA17_Chr1g0181511 26.613 124 66 1 39 137 27 150 8.17e-15 72.0
Msa0150190 MtrunA17_Chr2g0292101 31.783 129 62 2 39 141 25 153 9.42e-15 72.0
Msa0150190 MtrunA17_Chr3g0106901 26.512 215 112 4 10 198 11 205 1.25e-14 71.6
Msa0150190 MtrunA17_Chr4g0072091 32.800 125 58 2 39 137 24 148 2.23e-14 70.9
Msa0150190 MtrunA17_Chr4g0072091 52.459 61 29 0 142 202 263 323 1.47e-11 62.8
Msa0150190 MtrunA17_Chr1g0164891 30.081 123 57 2 43 136 93 215 2.86e-14 70.9
Msa0150190 MtrunA17_Chr4g0074851 28.800 125 64 2 38 137 31 155 3.76e-14 70.1
Msa0150190 MtrunA17_Chr3g0114491 31.148 122 57 2 25 120 5 125 4.84e-14 66.6
Msa0150190 MtrunA17_Chr3g0129061 29.448 163 78 3 1 137 1 152 8.35e-14 69.3
Msa0150190 MtrunA17_Chr1g0204011 27.404 208 105 5 9 191 6 192 1.28e-13 68.6
Msa0150190 MtrunA17_Chr2g0311251 35.606 132 47 6 39 138 11 136 1.75e-13 68.6
Msa0150190 MtrunA17_Chr4g0013181 28.455 123 61 2 42 137 62 184 6.16e-13 67.0
Msa0150190 MtrunA17_Chr6g0451571 27.778 126 63 2 39 136 34 159 4.00e-12 64.3
Msa0150190 MtrunA17_Chr2g0329641 27.517 149 84 3 13 138 11 158 6.63e-12 63.9
Msa0150190 MtrunA17_Chr7g0254181 34.375 96 51 2 46 141 4 87 6.66e-12 61.6
Msa0150190 MtrunA17_Chr7g0268501 29.252 147 72 6 17 137 6 146 6.74e-12 63.5
Msa0150190 MtrunA17_Chr7g0268521 29.252 147 72 6 17 137 6 146 6.74e-12 63.5
Msa0150190 MtrunA17_Chr5g0432351 27.451 153 75 4 14 137 7 152 1.72e-11 62.4
Msa0150190 MtrunA17_Chr5g0432331 28.750 160 79 5 7 137 30 183 4.60e-11 61.6
Msa0150190 MtrunA17_Chr5g0432341 29.412 153 72 5 14 137 7 152 8.07e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0150190 AT1G71695.1 35.849 212 93 4 13 191 12 213 2.09e-34 126
Msa0150190 AT1G71695.1 47.126 87 44 1 136 222 273 357 5.50e-16 75.9
Msa0150190 AT5G64120.1 37.415 147 70 1 13 137 9 155 5.84e-25 100
Msa0150190 AT3G17070.1 30.769 182 94 4 4 159 7 182 1.50e-23 96.7
Msa0150190 AT1G49570.1 39.683 126 50 1 39 138 48 173 4.81e-22 92.8
Msa0150190 AT1G68850.1 34.211 152 73 3 12 137 4 154 1.17e-21 91.7
Msa0150190 AT2G41480.1 41.880 117 46 2 43 137 33 149 2.18e-21 90.9
Msa0150190 AT2G34060.1 30.531 226 108 8 1 191 1 212 2.79e-21 90.9
Msa0150190 AT5G14130.1 30.818 159 75 2 6 138 8 157 3.45e-21 90.1
Msa0150190 AT2G18140.1 36.220 127 55 1 37 137 33 159 3.82e-21 90.1
Msa0150190 AT2G18140.1 34.862 109 63 3 96 203 235 336 2.13e-11 62.8
Msa0150190 AT5G14130.2 31.447 159 74 2 6 138 49 198 5.06e-21 90.1
Msa0150190 AT2G22420.1 29.108 213 105 5 14 200 7 199 7.99e-21 89.4
Msa0150190 AT5G15180.1 34.000 150 72 3 16 141 12 158 1.54e-20 88.6
Msa0150190 AT5G05340.1 33.600 125 57 1 39 137 30 154 1.80e-20 88.2
Msa0150190 AT3G49120.1 34.868 152 67 2 13 138 12 157 2.15e-20 88.2
Msa0150190 AT3G49110.1 34.868 152 67 3 13 138 13 158 2.96e-20 87.8
Msa0150190 AT4G33870.1 38.710 124 51 2 39 137 68 191 3.24e-20 88.2
Msa0150190 AT3G32980.1 35.897 156 68 2 9 138 7 156 3.97e-20 87.4
Msa0150190 AT5G06730.1 27.391 230 124 6 1 204 1 213 7.79e-20 87.0
Msa0150190 AT5G47000.1 33.939 165 76 2 1 138 1 159 9.91e-20 86.3
Msa0150190 AT2G24800.2 31.609 174 86 3 39 190 31 193 3.53e-19 83.2
Msa0150190 AT1G44970.1 36.364 121 51 1 43 137 49 169 5.31e-19 84.3
Msa0150190 AT5G19890.1 35.772 123 56 1 39 138 30 152 6.88e-19 84.0
Msa0150190 AT2G18150.1 31.447 159 81 3 7 137 2 160 7.44e-19 84.0
Msa0150190 AT2G24800.1 37.190 121 54 1 39 137 31 151 3.45e-18 82.0
Msa0150190 AT4G31760.1 27.778 216 113 4 11 204 11 205 3.82e-18 81.6
Msa0150190 AT5G64110.1 37.607 117 51 1 43 137 38 154 4.09e-18 81.6
Msa0150190 AT4G36430.1 33.884 121 54 1 43 137 34 154 5.62e-18 81.3
Msa0150190 AT5G06720.1 28.743 167 86 3 1 141 1 160 6.06e-18 81.3
Msa0150190 AT5G66390.1 33.884 121 54 1 43 137 37 157 6.76e-18 81.3
Msa0150190 AT5G64100.1 34.615 156 77 3 7 137 2 157 6.79e-18 81.3
Msa0150190 AT4G26010.2 32.051 156 69 2 11 141 34 177 8.39e-18 80.9
Msa0150190 AT5G39580.2 31.757 148 70 2 11 136 6 144 8.92e-18 79.3
Msa0150190 AT4G26010.1 32.051 156 69 2 11 141 6 149 9.02e-18 80.5
Msa0150190 AT4G08780.1 36.066 122 52 1 43 138 28 149 9.60e-18 80.9
Msa0150190 AT4G08780.1 35.766 137 63 6 96 222 225 346 1.83e-11 63.2
Msa0150190 AT5G39580.1 31.544 149 71 2 11 137 6 145 1.61e-17 80.1
Msa0150190 AT4G16270.1 36.800 125 53 2 39 137 50 174 1.78e-17 80.1
Msa0150190 AT1G77100.1 33.871 124 58 1 39 138 24 147 3.18e-17 79.3
Msa0150190 AT2G43480.1 31.013 158 79 4 21 154 17 168 3.93e-17 79.0
Msa0150190 AT1G05240.1 29.319 191 98 5 39 204 26 204 5.47e-17 78.6
Msa0150190 AT1G05250.1 29.319 191 98 5 39 204 26 204 5.47e-17 78.6
Msa0150190 AT1G05260.1 28.421 190 101 4 39 204 26 204 6.16e-17 78.6
Msa0150190 AT3G03670.1 31.298 131 65 1 33 138 17 147 6.57e-17 78.2
Msa0150190 AT2G35380.1 36.641 131 53 3 34 138 29 155 7.39e-17 78.2
Msa0150190 AT5G17820.1 30.055 183 97 4 33 193 18 191 9.18e-17 77.8
Msa0150190 AT4G08770.1 34.426 122 54 1 43 138 28 149 1.09e-16 77.8
Msa0150190 AT4G08770.1 36.496 137 62 6 96 222 225 346 4.69e-13 67.8
Msa0150190 AT5G22410.1 33.898 118 54 2 43 136 32 149 1.61e-16 77.4
Msa0150190 AT2G38390.1 34.959 123 54 2 42 138 34 156 1.65e-16 77.4
Msa0150190 AT3G01190.1 33.600 125 59 2 37 137 24 148 1.71e-16 77.0
Msa0150190 AT4G17690.1 33.333 135 60 2 31 138 21 152 1.73e-16 77.0
Msa0150190 AT3G50990.1 30.233 129 64 1 35 137 37 165 2.08e-16 77.0
Msa0150190 AT5G17820.2 30.055 183 97 4 33 193 71 244 2.51e-16 77.0
Msa0150190 AT5G58400.1 28.351 194 101 5 1 166 1 184 2.65e-16 76.6
Msa0150190 AT5G19880.1 34.646 127 55 2 39 137 25 151 4.85e-16 75.9
Msa0150190 AT5G51890.1 34.109 129 60 2 34 137 21 149 7.75e-16 75.5
Msa0150190 AT1G14540.1 30.323 155 73 3 11 139 2 147 9.46e-16 75.1
Msa0150190 AT5G24070.1 30.818 159 80 4 13 141 3 161 1.12e-15 75.1
Msa0150190 AT5G58390.1 27.230 213 113 4 18 204 6 202 1.80e-15 74.3
Msa0150190 AT4G25980.2 32.000 125 62 2 35 136 67 191 3.57e-15 73.2
Msa0150190 AT4G11290.1 30.400 125 61 2 39 137 25 149 5.08e-15 73.2
Msa0150190 AT2G38380.1 31.081 148 70 2 17 138 15 156 5.33e-15 73.2
Msa0150190 AT4G21960.1 29.530 149 80 2 15 138 7 155 5.49e-15 73.2
Msa0150190 AT4G25980.1 32.787 122 59 2 39 137 26 147 6.19e-15 72.8
Msa0150190 AT1G24110.1 31.544 149 71 2 17 138 4 148 1.30e-14 72.0
Msa0150190 AT2G39040.1 35.714 126 54 3 39 137 46 171 1.49e-14 72.0
Msa0150190 AT1G14550.1 31.373 153 72 4 11 137 5 150 3.55e-14 70.5
Msa0150190 AT1G30870.1 27.660 188 102 3 39 204 51 226 3.83e-14 70.9
Msa0150190 AT2G37130.1 29.600 125 62 3 39 137 30 154 3.66e-13 67.8
Msa0150190 AT2G37130.2 29.508 122 60 3 42 137 2 123 1.37e-12 65.9

Find 38 sgRNAs with CRISPR-Local

Find 241 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCTTAGATTTCCTATTATT+TGG 0.128939 1_4:-11478543 None:intergenic
CCCAATGGCACGTCAAAATC+TGG 0.255614 1_4:-11478011 None:intergenic
ATGCGTTTACATTGAAGTTT+AGG 0.301088 1_4:-11478046 None:intergenic
CTTACAATCCTTTCAATCCT+TGG 0.318088 1_4:-11475474 None:intergenic
TCCAAATAATAGGAAATCTA+AGG 0.367020 1_4:+11478542 Msa0150190:CDS
TTCACAATCTCCTTAGTCTT+AGG 0.369697 1_4:-11478402 None:intergenic
CTTGAATTTGTACAGTTTGG+AGG 0.394165 1_4:+11477987 Msa0150190:intron
GACTAGACTATGATTTCTAT+TGG 0.405781 1_4:+11475442 Msa0150190:CDS
GGCAAGTCATCCTAAGACTA+AGG 0.457751 1_4:+11478392 Msa0150190:CDS
CAAGGATTGAAAGGATTGTA+AGG 0.463937 1_4:+11475475 Msa0150190:CDS
CAACTCGTGGACATTGCTTA+CGG 0.486794 1_4:-11476463 None:intergenic
GGCATCTGTGGTGGAAGAGG+TGG 0.493676 1_4:+11478566 Msa0150190:CDS
CCAATGGCACGTCAAAATCT+GGG 0.508698 1_4:-11478010 None:intergenic
ATCTGTGGTGGAAGAGGTGG+TGG 0.511426 1_4:+11478569 Msa0150190:CDS
AAGGATAGTGGTCAAGCTCC+TGG 0.512801 1_4:+11475522 Msa0150190:CDS
GGTGGCATCTGTGGTGGAAG+AGG 0.513042 1_4:+11478563 Msa0150190:CDS
AGCAAGGCCTCCTGCTGTGA+GGG 0.513672 1_4:+11475419 Msa0150190:CDS
ATACCTGGGAAAAGCAATCA+TGG 0.522677 1_4:-11475563 None:intergenic
TCTATTGGTTGTCATGTCCA+AGG 0.523388 1_4:+11475457 Msa0150190:CDS
TAGTCTAGTCCCCTCACAGC+AGG 0.532120 1_4:-11475429 None:intergenic
CTTCGCGTGCGGCGATGACA+AGG 0.534915 1_4:-11476502 None:intergenic
GTTTACATCGGATCAAGACT+TGG 0.534996 1_4:+11478371 Msa0150190:CDS
GAAATCTAAGGTGGCATCTG+TGG 0.537357 1_4:+11478554 Msa0150190:CDS
CTACTCTCTCAGAGGAAACA+AGG 0.549748 1_4:-11475398 None:intergenic
TGTCATGTCCAAGGATTGAA+AGG 0.555771 1_4:+11475466 Msa0150190:CDS
ATCTAAGGTGGCATCTGTGG+TGG 0.560592 1_4:+11478557 Msa0150190:CDS
CTTTCTGGTTAGAAGCAAAG+TGG 0.576026 1_4:-11478425 None:intergenic
TCTGCGCACGAGACAACTCG+TGG 0.586025 1_4:-11476476 None:intergenic
TTGGCGAACAGCTTCGCGTG+CGG 0.588097 1_4:-11476513 None:intergenic
TATAGCACGAAGGTTCTCAA+TGG 0.596636 1_4:-11476438 None:intergenic
GCAAGGCCTCCTGCTGTGAG+GGG 0.634072 1_4:+11475420 Msa0150190:CDS
TCTAGTCCCCTCACAGCAGG+AGG 0.636097 1_4:-11475426 None:intergenic
AAATAATAGGAAATCTAAGG+TGG 0.648910 1_4:+11478545 Msa0150190:CDS
TAGCAAGGCCTCCTGCTGTG+AGG 0.654852 1_4:+11475418 Msa0150190:CDS
TCACTAGGGTATGTTTACAT+CGG 0.659677 1_4:+11478359 Msa0150190:intron
TTTCCTCTGAGAGAGTAGCA+AGG 0.674398 1_4:+11475403 Msa0150190:CDS
AGGCCTTGCTACTCTCTCAG+AGG 0.695047 1_4:-11475406 None:intergenic
GCTTACGGATTATAGCACGA+AGG 0.709375 1_4:-11476448 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATATATGACATTTATAAT+TGG + chr1_4:11476605-11476624 Msa0150190:intron 10.0%
!! ATACTATAAATTTATTTCAA+GGG - chr1_4:11476895-11476914 None:intergenic 10.0%
!! ATTTATAGTATCAAAATATT+TGG + chr1_4:11476904-11476923 Msa0150190:intron 10.0%
!!! GACTTAAAATAATTTTTTTT+TGG - chr1_4:11477147-11477166 None:intergenic 10.0%
!!! ACAGATCATATTTTATTAAA+TGG + chr1_4:11475695-11475714 Msa0150190:intron 15.0%
!!! TGGATTTATATTTTTTTAAG+AGG + chr1_4:11475767-11475786 Msa0150190:intron 15.0%
!!! AAAGTTAATTTTGAACAAAA+AGG + chr1_4:11476000-11476019 Msa0150190:intron 15.0%
!! TCTGAAGAAAAAAAAATTAA+AGG - chr1_4:11476100-11476119 None:intergenic 15.0%
!! AAATATTCATGGAAATAAAT+GGG - chr1_4:11476139-11476158 None:intergenic 15.0%
!! AAAATATTCATGGAAATAAA+TGG - chr1_4:11476140-11476159 None:intergenic 15.0%
!!! AAAGATTAAATGTTGATTTT+TGG + chr1_4:11476308-11476327 Msa0150190:intron 15.0%
!! ACACATAAATATAAATTACT+TGG - chr1_4:11476535-11476554 None:intergenic 15.0%
!! TACTATAAATTTATTTCAAG+GGG - chr1_4:11476894-11476913 None:intergenic 15.0%
!! GATACTATAAATTTATTTCA+AGG - chr1_4:11476896-11476915 None:intergenic 15.0%
!!! TGTATTATTTTCACTTTATA+CGG + chr1_4:11477199-11477218 Msa0150190:intron 15.0%
!!! TCAATATATTGTTTTAGTTA+AGG - chr1_4:11477240-11477259 None:intergenic 15.0%
!! AAAATCGTATAACAAAAGAA+TGG + chr1_4:11475723-11475742 Msa0150190:intron 20.0%
!!! TTTTGTTTTTCTTATGTGTT+GGG - chr1_4:11475906-11475925 None:intergenic 20.0%
!!! ATTTTGTTTTTCTTATGTGT+TGG - chr1_4:11475907-11475926 None:intergenic 20.0%
!!! TGTTTTTCTCTATTACTAAT+AGG - chr1_4:11476046-11476065 None:intergenic 20.0%
!! TGTAAATAAAAAGATTGTGT+AGG - chr1_4:11476249-11476268 None:intergenic 20.0%
!! ACTATAAATTTATTTCAAGG+GGG - chr1_4:11476893-11476912 None:intergenic 20.0%
!!! AATTTTTTTTTGGTGTAAAC+CGG - chr1_4:11477137-11477156 None:intergenic 20.0%
!! ATAAAATTATTTACACTGAC+AGG + chr1_4:11477173-11477192 Msa0150190:intron 20.0%
!! TAAAATTATTTACACTGACA+GGG + chr1_4:11477174-11477193 Msa0150190:intron 20.0%
!!! ATATGCTTAATTGCATTTTT+AGG - chr1_4:11477264-11477283 None:intergenic 20.0%
!!! AATTTTTTTTGATCCGAAAA+CGG - chr1_4:11478117-11478136 None:intergenic 20.0%
!!! ATTTGTTAAAAAACTCTTTC+TGG - chr1_4:11478443-11478462 None:intergenic 20.0%
!!! TCTTGAAGATGTTTTTGAAA+AGG + chr1_4:11475503-11475522 Msa0150190:CDS 25.0%
!! ACGTTGATAAAAAGAAAGTA+AGG - chr1_4:11475610-11475629 None:intergenic 25.0%
!!! CCTATTGAATCATTTTTTAG+AGG - chr1_4:11475645-11475664 None:intergenic 25.0%
!! CCTCTAAAAAATGATTCAAT+AGG + chr1_4:11475642-11475661 Msa0150190:intron 25.0%
! CTAATTTAGACCTGATATAA+CGG - chr1_4:11475675-11475694 None:intergenic 25.0%
!! ACAAAAACTCGATTTTTGAA+TGG + chr1_4:11475970-11475989 Msa0150190:intron 25.0%
! TATTACTAATAGGCTTAACT+TGG - chr1_4:11476036-11476055 None:intergenic 25.0%
!!! TTGATTTTTGGATTGAAACA+TGG + chr1_4:11476320-11476339 Msa0150190:intron 25.0%
!!! ATTTTGTTGAATGGTGTTAA+CGG + chr1_4:11476356-11476375 Msa0150190:intron 25.0%
! CTTTGCGATTAAAATAATTG+AGG - chr1_4:11476723-11476742 None:intergenic 25.0%
!!! GAACATCATTCATACTTTTT+TGG + chr1_4:11477291-11477310 Msa0150190:intron 25.0%
! AAGTGACATAATGTTAAATG+TGG - chr1_4:11477317-11477336 None:intergenic 25.0%
! TGATTGAGAATACAAACAAA+AGG + chr1_4:11477401-11477420 Msa0150190:intron 25.0%
!!! TTTTTAGGGTGTTTATTTTC+GGG - chr1_4:11477447-11477466 None:intergenic 25.0%
!!! GTTTTTAGGGTGTTTATTTT+CGG - chr1_4:11477448-11477467 None:intergenic 25.0%
! ACTTTATGACCAAATCAAAA+GGG + chr1_4:11477785-11477804 Msa0150190:intron 25.0%
!! TATGAACTATTAACCGTTTT+CGG + chr1_4:11478101-11478120 Msa0150190:intron 25.0%
! TACATATTATCGAATGTGTT+TGG - chr1_4:11478321-11478340 None:intergenic 25.0%
!!! ATTTGCTAATGCTTTTGTTA+AGG + chr1_4:11478461-11478480 Msa0150190:CDS 25.0%
!!! TTTTGTTAAGGTTAGTCAAT+TGG + chr1_4:11478473-11478492 Msa0150190:CDS 25.0%
! TCCAAATAATAGGAAATCTA+AGG + chr1_4:11478542-11478561 Msa0150190:CDS 25.0%
! ACCTTAGATTTCCTATTATT+TGG - chr1_4:11478546-11478565 None:intergenic 25.0%
! AAATAATAGGAAATCTAAGG+TGG + chr1_4:11478545-11478564 Msa0150190:CDS 25.0%
GACTAGACTATGATTTCTAT+TGG + chr1_4:11475442-11475461 Msa0150190:CDS 30.0%
!! GATGTTTTTGAAAAGGATAG+TGG + chr1_4:11475510-11475529 Msa0150190:CDS 30.0%
AAGAATCTTAAGTCATACCT+GGG - chr1_4:11475580-11475599 None:intergenic 30.0%
ATCGTATAACAAAAGAATGG+AGG + chr1_4:11475726-11475745 Msa0150190:intron 30.0%
TTCAATCTTAGCACTTCTTA+TGG - chr1_4:11475827-11475846 None:intergenic 30.0%
!! TAAGAAGTGCTAAGATTGAA+AGG + chr1_4:11475827-11475846 Msa0150190:intron 30.0%
AAATATGTTGTGACTACTCT+TGG - chr1_4:11475930-11475949 None:intergenic 30.0%
! AAAACTCGATTTTTGAATGG+AGG + chr1_4:11475973-11475992 Msa0150190:intron 30.0%
!! TAATAGGCTTAACTTGGTTT+TGG - chr1_4:11476030-11476049 None:intergenic 30.0%
TGCAGTCACAAAAATATTCA+TGG - chr1_4:11476150-11476169 None:intergenic 30.0%
! GATGCATCTATTTTGTTGAA+TGG + chr1_4:11476347-11476366 Msa0150190:intron 30.0%
TAACAAACCAGGTGATAAAT+AGG - chr1_4:11476837-11476856 None:intergenic 30.0%
TTGTTTGTATTCTCAATCAC+CGG - chr1_4:11477400-11477419 None:intergenic 30.0%
!!! TTTTAGGGGTGTTTATCTTT+GGG - chr1_4:11477579-11477598 None:intergenic 30.0%
!!! TTTTTAGGGGTGTTTATCTT+TGG - chr1_4:11477580-11477599 None:intergenic 30.0%
!! ATCACTTTTGATTCAAGATC+GGG - chr1_4:11477754-11477773 None:intergenic 30.0%
!! GATCACTTTTGATTCAAGAT+CGG - chr1_4:11477755-11477774 None:intergenic 30.0%
GATCTTGAATCAAAAGTGAT+CGG + chr1_4:11477754-11477773 Msa0150190:intron 30.0%
!! TTTGATTTGGTCATAAAGTC+TGG - chr1_4:11477784-11477803 None:intergenic 30.0%
GACTTTATGACCAAATCAAA+AGG + chr1_4:11477784-11477803 Msa0150190:intron 30.0%
! TGTCTAACTGCAATTTTGTT+TGG - chr1_4:11477852-11477871 None:intergenic 30.0%
! GTTAATTTTGCTTCAAGATC+GGG - chr1_4:11477881-11477900 None:intergenic 30.0%
! GGTTAATTTTGCTTCAAGAT+CGG - chr1_4:11477882-11477901 None:intergenic 30.0%
GATCTTGAAGCAAAATTAAC+CGG + chr1_4:11477881-11477900 Msa0150190:intron 30.0%
!!! CTTTTTTGTGTGCTGATTTT+CGG - chr1_4:11477911-11477930 None:intergenic 30.0%
TTCCTTGAATTTGTACAGTT+TGG + chr1_4:11477984-11478003 Msa0150190:intron 30.0%
ATGCGTTTACATTGAAGTTT+AGG - chr1_4:11478049-11478068 None:intergenic 30.0%
! TTCTTGAAATTTGGTTCGAT+TGG - chr1_4:11478234-11478253 None:intergenic 30.0%
!! TCGAGTTGTTTCTTGAAATT+TGG - chr1_4:11478243-11478262 None:intergenic 30.0%
TGTTCGAAGATCCAAATTAA+CGG - chr1_4:11478298-11478317 None:intergenic 30.0%
ATTAACCTCTTGAATCACTA+GGG + chr1_4:11478345-11478364 Msa0150190:intron 30.0%
! CATGTTTTGCTCCAAATAAT+AGG + chr1_4:11478532-11478551 Msa0150190:CDS 30.0%
CTTACAATCCTTTCAATCCT+TGG - chr1_4:11475477-11475496 None:intergenic 35.0%
CAAGGATTGAAAGGATTGTA+AGG + chr1_4:11475475-11475494 Msa0150190:CDS 35.0%
GAAGAATCTTAAGTCATACC+TGG - chr1_4:11475581-11475600 None:intergenic 35.0%
ATTTAGACCTGATATAACGG+TGG - chr1_4:11475672-11475691 None:intergenic 35.0%
CAAAATGCGCCAAAACATAT+CGG + chr1_4:11475871-11475890 Msa0150190:intron 35.0%
! TTGATATCGCCGATATGTTT+TGG - chr1_4:11475883-11475902 None:intergenic 35.0%
GTCACAACATATTTCACAAG+TGG + chr1_4:11475936-11475955 Msa0150190:intron 35.0%
!! ATAATTGAGTTGACATCTCG+TGG + chr1_4:11476215-11476234 Msa0150190:intron 35.0%
AATTGAGGCATGTAAGATCA+AGG - chr1_4:11476708-11476727 None:intergenic 35.0%
! ATTTCAAGGGGGTATAAGAA+TGG - chr1_4:11476882-11476901 None:intergenic 35.0%
CAACAGCGTTAAAACTCTTA+GGG + chr1_4:11477042-11477061 Msa0150190:intron 35.0%
!! GTTGGATATCGTTTTTACAG+TGG - chr1_4:11477535-11477554 None:intergenic 35.0%
!! TTTAGGGGTGTTTATCTTTG+GGG - chr1_4:11477578-11477597 None:intergenic 35.0%
!!! ACGGGAGGTTTATTTTTTAG+GGG - chr1_4:11477593-11477612 None:intergenic 35.0%
!!! GACGGGAGGTTTATTTTTTA+GGG - chr1_4:11477594-11477613 None:intergenic 35.0%
! CTTTTTATGGTTTCTTTCGG+TGG - chr1_4:11477697-11477716 None:intergenic 35.0%
! GGGCTTTTTATGGTTTCTTT+CGG - chr1_4:11477700-11477719 None:intergenic 35.0%
!!! TTGAGGTTTTGGGCTTTTTA+TGG - chr1_4:11477710-11477729 None:intergenic 35.0%
!! CAATTTTGTTTGGCGTGATT+TGG - chr1_4:11477842-11477861 None:intergenic 35.0%
ACAAATGACATGATATGCAG+TGG + chr1_4:11477935-11477954 Msa0150190:intron 35.0%
GCAATTAAGTGACGTAATCT+GGG - chr1_4:11477964-11477983 None:intergenic 35.0%
AGCAATTAAGTGACGTAATC+TGG - chr1_4:11477965-11477984 None:intergenic 35.0%
CTCCAAACTGTACAAATTCA+AGG - chr1_4:11477989-11478008 None:intergenic 35.0%
CTTGAATTTGTACAGTTTGG+AGG + chr1_4:11477987-11478006 Msa0150190:intron 35.0%
AGTTCATATGTTCGTGCAAA+TGG - chr1_4:11478089-11478108 None:intergenic 35.0%
!! ATGATTCGGTTGAACATTGT+AGG - chr1_4:11478198-11478217 None:intergenic 35.0%
! TTGAAATTTGGTTCGATTGG+CGG - chr1_4:11478231-11478250 None:intergenic 35.0%
CATTAACCTCTTGAATCACT+AGG + chr1_4:11478344-11478363 Msa0150190:intron 35.0%
TCACTAGGGTATGTTTACAT+CGG + chr1_4:11478359-11478378 Msa0150190:intron 35.0%
! TTCACAATCTCCTTAGTCTT+AGG - chr1_4:11478405-11478424 None:intergenic 35.0%
!!! AGTCAATTGGATGTTTTGAC+TGG + chr1_4:11478486-11478505 Msa0150190:CDS 35.0%
!!! GATGTTTTGACTGGAAATCA+AGG + chr1_4:11478495-11478514 Msa0150190:CDS 35.0%
! TCTATTGGTTGTCATGTCCA+AGG + chr1_4:11475457-11475476 Msa0150190:CDS 40.0%
TGTCATGTCCAAGGATTGAA+AGG + chr1_4:11475466-11475485 Msa0150190:CDS 40.0%
AAAAAGAGTCTGAGTATGCC+AGG - chr1_4:11475543-11475562 None:intergenic 40.0%
!! TTTCCATGATTGCTTTTCCC+AGG + chr1_4:11475560-11475579 Msa0150190:CDS 40.0%
ATACCTGGGAAAAGCAATCA+TGG - chr1_4:11475566-11475585 None:intergenic 40.0%
!! GGAGAGCAAAAGTATTAACG+TGG + chr1_4:11475747-11475766 Msa0150190:intron 40.0%
TATAGCACGAAGGTTCTCAA+TGG - chr1_4:11476441-11476460 None:intergenic 40.0%
CTCTCAACCTATTTATCACC+TGG + chr1_4:11476827-11476846 Msa0150190:intron 40.0%
! GTTTGTTAACTCCCTTGTCT+AGG + chr1_4:11476849-11476868 Msa0150190:intron 40.0%
!!! ATGGTTTTGTGCCTAGACAA+GGG - chr1_4:11476863-11476882 None:intergenic 40.0%
!!! AATGGTTTTGTGCCTAGACA+AGG - chr1_4:11476864-11476883 None:intergenic 40.0%
GCAACAGCGTTAAAACTCTT+AGG + chr1_4:11477041-11477060 Msa0150190:intron 40.0%
!! GGAGAGCTTGTTATCCATTT+AGG + chr1_4:11477063-11477082 Msa0150190:intron 40.0%
!! AAACAAAAGGCGCCGTATAT+AGG + chr1_4:11477414-11477433 Msa0150190:intron 40.0%
!!! TGACGAGTATTTTGATGGTG+TGG - chr1_4:11477482-11477501 None:intergenic 40.0%
! TTCGTGAAGGAGTCTGTTTT+AGG - chr1_4:11477508-11477527 None:intergenic 40.0%
!! GGATATCGTTTTTACAGTGG+TGG - chr1_4:11477532-11477551 None:intergenic 40.0%
!! TTAGGGGTGTTTATCTTTGG+GGG - chr1_4:11477577-11477596 None:intergenic 40.0%
!!! GGACGGGAGGTTTATTTTTT+AGG - chr1_4:11477595-11477614 None:intergenic 40.0%
TTTCGATTGTGGCTCAACTT+TGG - chr1_4:11477624-11477643 None:intergenic 40.0%
AGTTGAATGGTCTTTCGGTT+AGG - chr1_4:11477651-11477670 None:intergenic 40.0%
GTGGAAGTTGAATGGTCTTT+CGG - chr1_4:11477656-11477675 None:intergenic 40.0%
TATGGTTTCTTTCGGTGGAT+CGG - chr1_4:11477692-11477711 None:intergenic 40.0%
!! TGGCTCAAGTTTGAGGTTTT+GGG - chr1_4:11477720-11477739 None:intergenic 40.0%
!! TTGGCTCAAGTTTGAGGTTT+TGG - chr1_4:11477721-11477740 None:intergenic 40.0%
! AGTTCTTTGGCTCAAGTTTG+AGG - chr1_4:11477727-11477746 None:intergenic 40.0%
!!! TTTGTGTGCTGATTTTCGGT+GGG - chr1_4:11477907-11477926 None:intergenic 40.0%
!!! TTTTGTGTGCTGATTTTCGG+TGG - chr1_4:11477908-11477927 None:intergenic 40.0%
AATGACATGATATGCAGTGG+TGG + chr1_4:11477938-11477957 Msa0150190:intron 40.0%
ATGTTCGTGCAAATGGTACT+GGG - chr1_4:11478082-11478101 None:intergenic 40.0%
TATGTTCGTGCAAATGGTAC+TGG - chr1_4:11478083-11478102 None:intergenic 40.0%
ACCAGATAGAGCAACTACAT+CGG - chr1_4:11478148-11478167 None:intergenic 40.0%
! TGAAATTTGGTTCGATTGGC+GGG - chr1_4:11478230-11478249 None:intergenic 40.0%
! ATCCAAATTAACGGCGTTGA+GGG - chr1_4:11478289-11478308 None:intergenic 40.0%
ACATACCCTAGTGATTCAAG+AGG - chr1_4:11478353-11478372 None:intergenic 40.0%
GTTTACATCGGATCAAGACT+TGG + chr1_4:11478371-11478390 Msa0150190:CDS 40.0%
!! CTTTCTGGTTAGAAGCAAAG+TGG - chr1_4:11478428-11478447 None:intergenic 40.0%
CTACTCTCTCAGAGGAAACA+AGG - chr1_4:11475401-11475420 None:intergenic 45.0%
! TTTCCTCTGAGAGAGTAGCA+AGG + chr1_4:11475403-11475422 Msa0150190:CDS 45.0%
AGGTGATCCACCGTTATATC+AGG + chr1_4:11475662-11475681 Msa0150190:intron 45.0%
! GAATGGTGTTAACGGAGAAG+AGG + chr1_4:11476364-11476383 Msa0150190:intron 45.0%
TGATGCCAACTTCGCTTTGA+AGG + chr1_4:11476400-11476419 Msa0150190:intron 45.0%
! GCTTACGGATTATAGCACGA+AGG - chr1_4:11476451-11476470 None:intergenic 45.0%
CAACTCGTGGACATTGCTTA+CGG - chr1_4:11476466-11476485 None:intergenic 45.0%
GACAAGGGAGTTAACAAACC+AGG - chr1_4:11476848-11476867 None:intergenic 45.0%
TTTAACGCTGTTGCATGCCA+AGG - chr1_4:11477035-11477054 None:intergenic 45.0%
! ACTAATCCCTCGAGCATTGT+GGG - chr1_4:11477090-11477109 None:intergenic 45.0%
!! TTTGATGGTGTGGTCCTCAT+GGG - chr1_4:11477472-11477491 None:intergenic 45.0%
!!! TTTTGATGGTGTGGTCCTCA+TGG - chr1_4:11477473-11477492 None:intergenic 45.0%
!! GGTGGTGACGAGTATTTTGA+TGG - chr1_4:11477487-11477506 None:intergenic 45.0%
!! GTGAAGGAGTCTGTTTTAGG+TGG - chr1_4:11477505-11477524 None:intergenic 45.0%
TTACAGTGGTGGTTTCGTGA+AGG - chr1_4:11477521-11477540 None:intergenic 45.0%
!! TCAACTTTGGTGTTCAGGAC+GGG - chr1_4:11477611-11477630 None:intergenic 45.0%
!! CTCAACTTTGGTGTTCAGGA+CGG - chr1_4:11477612-11477631 None:intergenic 45.0%
! GGTTAGGAGCTTTTCGATTG+TGG - chr1_4:11477635-11477654 None:intergenic 45.0%
AAGATCGGGAGTCAGTTCTT+TGG - chr1_4:11477740-11477759 None:intergenic 45.0%
! TTGGTGTTTCTCTGGTGTAC+CGG - chr1_4:11477823-11477842 None:intergenic 45.0%
TTTGCTTCAAGATCGGGAGT+CGG - chr1_4:11477875-11477894 None:intergenic 45.0%
CCAATGGCACGTCAAAATCT+GGG - chr1_4:11478013-11478032 None:intergenic 45.0%
!! CCAGATTTTGACGTGCCATT+GGG + chr1_4:11478011-11478030 Msa0150190:CDS 45.0%
! AGGCTGTCTTTTCTTCCCAA+TGG - chr1_4:11478029-11478048 None:intergenic 45.0%
ACCGATGTAGTTGCTCTATC+TGG + chr1_4:11478144-11478163 Msa0150190:intron 45.0%
! CTATCTGGTGCTCACACTTT+TGG + chr1_4:11478159-11478178 Msa0150190:intron 45.0%
ATCCCTCAACGCCGTTAATT+TGG + chr1_4:11478284-11478303 Msa0150190:intron 45.0%
GATCCAAATTAACGGCGTTG+AGG - chr1_4:11478290-11478309 None:intergenic 45.0%
GGCAAGTCATCCTAAGACTA+AGG + chr1_4:11478392-11478411 Msa0150190:CDS 45.0%
! GAAATCTAAGGTGGCATCTG+TGG + chr1_4:11478554-11478573 Msa0150190:CDS 45.0%
! AAGGATAGTGGTCAAGCTCC+TGG + chr1_4:11475522-11475541 Msa0150190:CDS 50.0%
!! TGCCAACTTCGCTTTGAAGG+AGG + chr1_4:11476403-11476422 Msa0150190:intron 50.0%
CTCCTCCTTCAAAGCGAAGT+TGG - chr1_4:11476408-11476427 None:intergenic 50.0%
!! CAACTTCGCTTTGAAGGAGG+AGG + chr1_4:11476406-11476425 Msa0150190:intron 50.0%
! CGAGCATTGTGGGACCTAAA+TGG - chr1_4:11477080-11477099 None:intergenic 50.0%
TTTAGGTCCCACAATGCTCG+AGG + chr1_4:11477080-11477099 Msa0150190:intron 50.0%
TTAGGTCCCACAATGCTCGA+GGG + chr1_4:11477081-11477100 Msa0150190:intron 50.0%
! GACTAATCCCTCGAGCATTG+TGG - chr1_4:11477091-11477110 None:intergenic 50.0%
GATCGTCTTGAGCAGGGATT+AGG - chr1_4:11477372-11477391 None:intergenic 50.0%
GTCTCTGATCGTCTTGAGCA+GGG - chr1_4:11477378-11477397 None:intergenic 50.0%
TGCTCAAGACGATCAGAGAC+CGG + chr1_4:11477378-11477397 Msa0150190:intron 50.0%
!! CAAAAGGCGCCGTATATAGG+CGG + chr1_4:11477417-11477436 Msa0150190:intron 50.0%
TCGGGTGTTCCGCCTATATA+CGG - chr1_4:11477429-11477448 None:intergenic 50.0%
ACACCCTAAAAACGCCCATG+AGG + chr1_4:11477455-11477474 Msa0150190:intron 50.0%
! GGTCCTCATGGGCGTTTTTA+GGG - chr1_4:11477461-11477480 None:intergenic 50.0%
! TGGTCCTCATGGGCGTTTTT+AGG - chr1_4:11477462-11477481 None:intergenic 50.0%
GAGTAGTGACGATGGATGGT+TGG - chr1_4:11477553-11477572 None:intergenic 50.0%
!! GTGGCTCAACTTTGGTGTTC+AGG - chr1_4:11477616-11477635 None:intergenic 50.0%
!! GAGAGGTGGTGGAAGTTGAA+TGG - chr1_4:11477664-11477683 None:intergenic 50.0%
!!! TGAGGGGTGCCCTTTTGATT+TGG - chr1_4:11477797-11477816 None:intergenic 50.0%
!! GGCGTGATTTGGTGTTTCTC+TGG - chr1_4:11477831-11477850 None:intergenic 50.0%
!! CCCAGATTTTGACGTGCCAT+TGG + chr1_4:11478010-11478029 Msa0150190:CDS 50.0%
CCCAATGGCACGTCAAAATC+TGG - chr1_4:11478014-11478033 None:intergenic 50.0%
GCGGGTCGGAATCAATGATT+CGG - chr1_4:11478212-11478231 None:intergenic 50.0%
! ATTTGGTTCGATTGGCGGGT+CGG - chr1_4:11478226-11478245 None:intergenic 50.0%
CGAAGCTACATGTCCGAATG+AGG + chr1_4:11478260-11478279 Msa0150190:intron 50.0%
GCGTTGAGGGATTCCTCATT+CGG - chr1_4:11478276-11478295 None:intergenic 50.0%
! ATCTAAGGTGGCATCTGTGG+TGG + chr1_4:11478557-11478576 Msa0150190:CDS 50.0%
AGGCCTTGCTACTCTCTCAG+AGG - chr1_4:11475409-11475428 None:intergenic 55.0%
TAGTCTAGTCCCCTCACAGC+AGG - chr1_4:11475432-11475451 None:intergenic 55.0%
CCAAGGCTGGATTCGAACTC+AGG - chr1_4:11477018-11477037 None:intergenic 55.0%
CCTGAGTTCGAATCCAGCCT+TGG + chr1_4:11477015-11477034 Msa0150190:intron 55.0%
TTAGTCTCTGCAGTTGCGCG+TGG + chr1_4:11477105-11477124 Msa0150190:intron 55.0%
GGTCTCTGATCGTCTTGAGC+AGG - chr1_4:11477379-11477398 None:intergenic 55.0%
GGGTGAGTAGTGACGATGGA+TGG - chr1_4:11477557-11477576 None:intergenic 55.0%
TTGGGGGTGAGTAGTGACGA+TGG - chr1_4:11477561-11477580 None:intergenic 55.0%
!! ACTTTGGTGTTCAGGACGGG+AGG - chr1_4:11477608-11477627 None:intergenic 55.0%
GGTTTCTTTCGGTGGATCGG+AGG - chr1_4:11477689-11477708 None:intergenic 55.0%
!! AAAAGGGCACCCCTCACTTC+CGG + chr1_4:11477801-11477820 Msa0150190:intron 55.0%
TCTGGTGTACCGGAAGTGAG+GGG - chr1_4:11477813-11477832 None:intergenic 55.0%
CTCTGGTGTACCGGAAGTGA+GGG - chr1_4:11477814-11477833 None:intergenic 55.0%
TCTCTGGTGTACCGGAAGTG+AGG - chr1_4:11477815-11477834 None:intergenic 55.0%
!!! TGTGCTGATTTTCGGTGGGC+CGG - chr1_4:11477903-11477922 None:intergenic 55.0%
! ATCTGTGGTGGAAGAGGTGG+TGG + chr1_4:11478569-11478588 Msa0150190:CDS 55.0%
TAGCAAGGCCTCCTGCTGTG+AGG + chr1_4:11475418-11475437 Msa0150190:CDS 60.0%
AGCAAGGCCTCCTGCTGTGA+GGG + chr1_4:11475419-11475438 Msa0150190:CDS 60.0%
TCTAGTCCCCTCACAGCAGG+AGG - chr1_4:11475429-11475448 None:intergenic 60.0%
TCTGCGCACGAGACAACTCG+TGG - chr1_4:11476479-11476498 None:intergenic 60.0%
TTGGCGAACAGCTTCGCGTG+CGG - chr1_4:11476516-11476535 None:intergenic 60.0%
ACGCTGTTGCATGCCAAGGC+TGG - chr1_4:11477031-11477050 None:intergenic 60.0%
CAGTTGCGCGTGGAGGATAC+CGG + chr1_4:11477115-11477134 Msa0150190:intron 60.0%
! GGTGGCATCTGTGGTGGAAG+AGG + chr1_4:11478563-11478582 Msa0150190:CDS 60.0%
! GGCATCTGTGGTGGAAGAGG+TGG + chr1_4:11478566-11478585 Msa0150190:CDS 60.0%
GCAAGGCCTCCTGCTGTGAG+GGG + chr1_4:11475420-11475439 Msa0150190:CDS 65.0%
CTTCGCGTGCGGCGATGACA+AGG - chr1_4:11476505-11476524 None:intergenic 65.0%
GTCTCTGCAGTTGCGCGTGG+AGG + chr1_4:11477108-11477127 Msa0150190:intron 65.0%
GATCGGAGGTCGAGAGGTGG+TGG - chr1_4:11477675-11477694 None:intergenic 65.0%
GTGGATCGGAGGTCGAGAGG+TGG - chr1_4:11477678-11477697 None:intergenic 65.0%
TCGGTGGATCGGAGGTCGAG+AGG - chr1_4:11477681-11477700 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 11475330 11478611 11475330 ID=Msa0150190;Name=Msa0150190
chr1_4 mRNA 11475330 11478611 11475330 ID=Msa0150190-mRNA-1;Parent=Msa0150190;Name=Msa0150190-mRNA-1;_AED=0.20;_eAED=0.20;_QI=0|0|0|1|1|1|4|0|222
chr1_4 exon 11475330 11475581 11475330 ID=Msa0150190-mRNA-1:exon:4713;Parent=Msa0150190-mRNA-1
chr1_4 exon 11476422 11476535 11476422 ID=Msa0150190-mRNA-1:exon:4714;Parent=Msa0150190-mRNA-1
chr1_4 exon 11478002 11478058 11478002 ID=Msa0150190-mRNA-1:exon:4715;Parent=Msa0150190-mRNA-1
chr1_4 exon 11478366 11478611 11478366 ID=Msa0150190-mRNA-1:exon:4716;Parent=Msa0150190-mRNA-1
chr1_4 CDS 11475330 11475581 11475330 ID=Msa0150190-mRNA-1:cds;Parent=Msa0150190-mRNA-1
chr1_4 CDS 11476422 11476535 11476422 ID=Msa0150190-mRNA-1:cds;Parent=Msa0150190-mRNA-1
chr1_4 CDS 11478002 11478058 11478002 ID=Msa0150190-mRNA-1:cds;Parent=Msa0150190-mRNA-1
chr1_4 CDS 11478366 11478611 11478366 ID=Msa0150190-mRNA-1:cds;Parent=Msa0150190-mRNA-1
Gene Sequence

>Msa0150190

ATGGCTATGGTTAGTGCAAGTAGATCACATTCATTGTTTTTTATTTTATTTCTATTAGCTTCTCTAATCCTTGTTTCCTCTGAGAGAGTAGCAAGGCCTCCTGCTGTGAGGGGACTAGACTATGATTTCTATTGGTTGTCATGTCCAAGGATTGAAAGGATTGTAAGGAAACATCTTGAAGATGTTTTTGAAAAGGATAGTGGTCAAGCTCCTGGCATACTCAGACTCTTTTTCCATGATTGCTTTTCCCAGGAGGAGGCTCTTCAGACCATTGAGAACCTTCGTGCTATAATCCGTAAGCAATGTCCACGAGTTGTCTCGTGCGCAGATATCCTTGTCATCGCCGCACGCGAAGCTGTTCGCCAATTTGGAGGCCCAGATTTTGACGTGCCATTGGGAAGAAAAGACAGCCTAAACTTCAATGGTATGTTTACATCGGATCAAGACTTGGCAAGTCATCCTAAGACTAAGGAGATTGTGAACCACTTTGCTTCTAACCAGAAAGAGTTTTTTAACAAATTTGCTAATGCTTTTGTTAAGGTTAGTCAATTGGATGTTTTGACTGGAAATCAAGGAGAAATTCGTAAGTCATGTTTTGCTCCAAATAATAGGAAATCTAAGGTGGCATCTGTGGTGGAAGAGGTGGTGGAAATTGCAGCAAACATGTAG
Protein sequence

>Msa0150190

MAMVSASRSHSLFFILFLLASLILVSSERVARPPAVRGLDYDFYWLSCPRIERIVRKHLEDVFEKDSGQAPGILRLFFHDCFSQEEALQTIENLRAIIRKQCPRVVSCADILVIAAREAVRQFGGPDFDVPLGRKDSLNFNGMFTSDQDLASHPKTKEIVNHFASNQKEFFNKFANAFVKVSQLDVLTGNQGEIRKSCFAPNNRKSKVASVVEEVVEIAANM*