AlfalfaGEDB | Msa0153900

M. sativa cultivar zhongmu-4 / Msa0153900

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0153900	XP_024630184.1	85.294	136	20	0	4	139	59	194	4.21e-77	237

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0153900	A0A072TJ78	86.029	136	19	0	4	139	208	343	6.64e-77	242

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0153900	Msa0564510	0.802933	4.434438e-49	-8.615850e-47
Msa0153900	Msa0885280	0.817043	4.045233e-52	-8.615850e-47
Msa0153900	Msa0923540	0.821928	3.110963e-53	-8.615850e-47
Msa0153900	Msa1075080	-0.806562	7.743955e-50	-8.615850e-47
Msa0153900	Msa1136090	0.809641	1.711544e-50	-8.615850e-47
Msa0153900	Msa1327770	0.805384	1.370144e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0153900	MtrunA17_Chr1g0164121	83.824	136	22	0	4	139	172	307	6.17e-78	233
Msa0153900	MtrunA17_Chr8g0359891	86.131	137	19	0	4	140	2801	2937	1.33e-75	244
Msa0153900	MtrunA17_Chr8g0359891	42.963	135	75	2	5	139	1792	1924	5.69e-23	94.0
Msa0153900	MtrunA17_Chr8g0359891	42.222	135	76	2	5	139	2288	2420	1.62e-21	89.7
Msa0153900	MtrunA17_Chr8g0359891	38.571	140	81	2	2	139	1268	1404	2.43e-20	86.3
Msa0153900	MtrunA17_Chr8g0359891	37.143	140	83	2	2	139	427	563	6.77e-20	85.1
Msa0153900	MtrunA17_Chr1g0164011	85.926	135	19	0	5	139	2361	2495	2.82e-75	243
Msa0153900	MtrunA17_Chr1g0164011	45.185	135	72	2	5	139	1840	1972	1.00e-24	99.0
Msa0153900	MtrunA17_Chr1g0164011	37.143	140	83	2	2	139	1393	1529	2.99e-19	83.2
Msa0153900	MtrunA17_Chr1g0163891	87.500	136	17	0	5	140	2972	3107	2.92e-75	243

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 22 sgRNAs with CRISPR-Local

Find 22 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACATCGTATTTGAAAACC+TGG	0.549598	1_4:+17932373	Msa0153900:CDS
AACTAACTGTGAGAAGATGT+TGG	0.608661	1_4:+17932316	Msa0153900:CDS
AAGTAAATTCCAAGTATTCC+AGG	0.346947	1_4:-17932391	None:intergenic
ACAGTAGCACCGTGCCTCAC+AGG	0.560430	1_4:+17932527	Msa0153900:CDS
ACATCAATTCCTGTGAGGCA+CGG	0.617104	1_4:-17932536	None:intergenic
ACGACGAAACGAAGCAAAGA+TGG	0.445701	1_4:-17932464	None:intergenic
AGTTTGAGAAGCTTCTGCTA+TGG	0.535941	1_4:+17932422	Msa0153900:CDS
ATTTGAAAACCTGGAATACT+TGG	0.360265	1_4:+17932382	Msa0153900:CDS
CAGGTTGATCTATTTGAAAG+TGG	0.540090	1_4:+17932217	Msa0153900:CDS
CGACGAAACGAAGCAAAGAT+GGG	0.541623	1_4:-17932463	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GATTGATGAAGAAAAAACAG+AGG	+	chr1_4:17932349-17932368	Msa0153900:CDS	30.0%
AAACATCGTATTTGAAAACC+TGG	+	chr1_4:17932373-17932392	Msa0153900:CDS	30.0%
ATTTGAAAACCTGGAATACT+TGG	+	chr1_4:17932382-17932401	Msa0153900:CDS	30.0%
AAGTAAATTCCAAGTATTCC+AGG	-	chr1_4:17932394-17932413	None:intergenic	30.0%
GAACAAATGTTCACAGAAAA+AGG	+	chr1_4:17932605-17932624	Msa0153900:CDS	30.0%
AACTAACTGTGAGAAGATGT+TGG	+	chr1_4:17932316-17932335	Msa0153900:CDS	35.0%
GATGAGAAGATCTTCATTTG+AGG	-	chr1_4:17932500-17932519	None:intergenic	35.0%
GAAGAAAATATGCGATGGAA+AGG	+	chr1_4:17932563-17932582	Msa0153900:CDS	35.0%
GAAGCTTCTCAAACTTGACA+AGG	-	chr1_4:17932417-17932436	None:intergenic	40.0%
AGTTTGAGAAGCTTCTGCTA+TGG	+	chr1_4:17932422-17932441	Msa0153900:CDS	40.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_4	gene	17932209	17932652	17932209	ID=Msa0153900;Name=Msa0153900
chr1_4	mRNA	17932209	17932652	17932209	ID=Msa0153900-mRNA-1;Parent=Msa0153900;Name=Msa0153900-mRNA-1;_AED=0.48;_eAED=0.49;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|147
chr1_4	exon	17932209	17932652	17932209	ID=Msa0153900-mRNA-1:exon:6748;Parent=Msa0153900-mRNA-1
chr1_4	CDS	17932209	17932652	17932209	ID=Msa0153900-mRNA-1:cds;Parent=Msa0153900-mRNA-1

Gene Sequence

>Msa0153900

ATGATGCACAGGTTGATCTATTTGAAAGTGGACAACTGCAAAGAGTTGATCTATTTGATAACAATTTCAACAGCTAAAAGTCTAGTTCAACTCAAAGCATTAGAAGTAACTAACTGTGAGAAGATGTTGGATGTCGTGAAGATTGATGAAGAAAAAACAGAGGAAAACATCGTATTTGAAAACCTGGAATACTTGGAATTTACTTCCTTGTCAAGTTTGAGAAGCTTCTGCTATGGGAAACAAGCATTCATATTCCCATCTTTGCTTCGTTTCGTCGTTAAAGGATGTCCTCAAATGAAGATCTTCTCATCCGCACTCACAGTAGCACCGTGCCTCACAGGAATTGATGTGGGAGAAGAAAATATGCGATGGAAAGGTGATCTTAACACAACTATTGAACAAATGTTCACAGAAAAAGGTACACAATGTCATTATTATTTATGA

Protein sequence

>Msa0153900

MMHRLIYLKVDNCKELIYLITISTAKSLVQLKALEVTNCEKMLDVVKIDEEKTEENIVFENLEYLEFTSLSSLRSFCYGKQAFIFPSLLRFVVKGCPQMKIFSSALTVAPCLTGIDVGEENMRWKGDLNTTIEQMFTEKGTQCHYYL*