AlfalfaGEDB | Msa0163710

M. sativa cultivar zhongmu-4 / Msa0163710

KEGG: K20624
GO: -
PFAM: PF00067.25

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0163710	XP_013468024.1	95.699	372	15	1	1	372	144	514	0.0	732

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0163710	sp\|Q9ZUX1\|C94C1_ARATH	53.619	373	165	5	1	370	127	494	3.90e-137	402

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0163710	A0A072VKA5	95.699	372	15	1	1	372	144	514	0.0	732

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0024620	Msa0163710	0.918832	9.571493e-87	-8.615850e-47
Msa0072120	Msa0163710	0.908027	2.677345e-81	-8.615850e-47
Msa0120490	Msa0163710	0.891746	2.996514e-74	-8.615850e-47
Msa0163710	Msa0355200	0.805343	1.397395e-49	-8.615850e-47
Msa0163710	Msa0402260	0.805019	1.633707e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0163710	MtrunA17_Chr1g0178971	95.699	372	15	1	1	372	144	514	0.0	732
Msa0163710	MtrunA17_Chr4g0026941	57.031	384	142	5	1	370	165	539	4.06e-155	447
Msa0163710	MtrunA17_Chr2g0321171	55.041	367	155	3	1	367	139	495	3.81e-150	433
Msa0163710	MtrunA17_Chr7g0260831	43.799	379	192	6	1	370	136	502	2.10e-107	324
Msa0163710	MtrunA17_Chr7g0260821	43.421	380	192	7	1	370	137	503	1.33e-103	314
Msa0163710	MtrunA17_Chr7g0255131	43.799	379	196	7	2	370	137	508	1.75e-99	304
Msa0163710	MtrunA17_Chr7g0225531	40.260	385	202	6	1	370	140	511	2.71e-96	296
Msa0163710	MtrunA17_Chr3g0119681	40.103	389	212	5	1	373	137	520	7.82e-96	295
Msa0163710	MtrunA17_Chr1g0184461	41.406	384	201	8	1	373	137	507	2.19e-95	293
Msa0163710	MtrunA17_Chr1g0184421	41.207	381	200	8	1	370	137	504	5.43e-92	285

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0163710	AT2G27690.1	53.619	373	165	5	1	370	127	494	3.96e-138	402
Msa0163710	AT3G48520.1	42.782	381	193	6	1	370	133	499	3.74e-105	319
Msa0163710	AT5G63450.1	42.708	384	190	7	1	370	135	502	1.41e-101	310
Msa0163710	AT5G63450.2	42.708	384	190	7	1	370	193	560	7.22e-101	310
Msa0163710	AT1G34540.1	41.499	347	189	6	2	339	129	470	6.65e-90	279
Msa0163710	AT3G56630.1	41.159	345	193	6	2	339	129	470	8.38e-90	279
Msa0163710	AT5G08250.1	36.842	380	217	6	2	365	101	473	4.10e-87	272
Msa0163710	AT5G08250.2	36.340	388	224	6	2	373	163	543	1.96e-86	272
Msa0163710	AT3G01900.1	39.788	377	205	7	1	370	129	490	3.19e-86	270
Msa0163710	AT1G24540.1	36.856	388	219	7	1	370	141	520	7.56e-85	267

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Find 60 sgRNAs with CRISPR-Local

Find 162 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGTGAAAAGTGGTACTA+GGG	0.561980	1_4:-42044410	Msa0163710:CDS
AAAATCTCTTTCTCAACTTC+CGG	0.371569	1_4:+42044619	None:intergenic
AAACAACTTGGTCTAGGTTT+TGG	0.292314	1_4:+42045079	None:intergenic
AACTTTGAATCAAATTGAAT+TGG	0.350752	1_4:+42044466	None:intergenic
AAGGATGTTTCTAGACACAT+AGG	0.557282	1_4:+42044988	None:intergenic
AATTATTGGAGACACAACCA+TGG	0.692792	1_4:+42044906	None:intergenic
ACACATAGGGTCTAAACCAA+AGG	0.600563	1_4:+42045002	None:intergenic
ACTTATCATCCTTATGCAAT+GGG	0.453213	1_4:-42044385	Msa0163710:CDS
ACTTGTGAGTGATGATGCCA+CGG	0.691784	1_4:+42044666	None:intergenic
AGAAACATCCTTACCCATGT+CGG	0.620375	1_4:-42044977	Msa0163710:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTCAAAGGCAAAAGATCTTA+TGG	+	chr1_4:42044109-42044128	None:intergenic	30.0%
AATCAAAAACAACTTGGTCT+AGG	+	chr1_4:42044185-42044204	None:intergenic	30.0%
CTTGCAAATCAAAAACAACT+TGG	+	chr1_4:42044191-42044210	None:intergenic	30.0%
TGACAACAAATCTTTGTGAT+GGG	+	chr1_4:42044490-42044509	None:intergenic	30.0%
TTGACAACAAATCTTTGTGA+TGG	+	chr1_4:42044491-42044510	None:intergenic	30.0%
AAAATCTCTTTCTCAACTTC+CGG	+	chr1_4:42044639-42044658	None:intergenic	30.0%
TTAGAAGCTGAACAAGTAAT+TGG	-	chr1_4:42044660-42044679	Msa0163710:CDS	30.0%
AAAAGTGAAAAGTGGTACTA+GGG	-	chr1_4:42044845-42044864	Msa0163710:CDS	30.0%
TACTTATCATCCTTATGCAA+TGG	-	chr1_4:42044869-42044888	Msa0163710:CDS	30.0%
ACTTATCATCCTTATGCAAT+GGG	-	chr1_4:42044870-42044889	Msa0163710:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_4	gene	42044078	42045199	42044078	ID=Msa0163710;Name=Msa0163710
chr1_4	mRNA	42044078	42045199	42044078	ID=Msa0163710-mRNA-1;Parent=Msa0163710;Name=Msa0163710-mRNA-1;_AED=0.14;_eAED=0.14;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|373
chr1_4	exon	42044078	42045199	42044078	ID=Msa0163710-mRNA-1:exon:12185;Parent=Msa0163710-mRNA-1
chr1_4	CDS	42044078	42045199	42044078	ID=Msa0163710-mRNA-1:cds;Parent=Msa0163710-mRNA-1

Gene Sequence

>Msa0163710

ATGGCAAGTTTAGAACTCAACAAACAATCCATAAGATCTTTTGCCTTTGAAGTTGTCAACAACGAAATCCAACATCGACTCATCCCTCTACTCCAAAACCAAAACCTAGACCAAGTTGTTTTTGATTTGCAAGATGTTTTCAAAAGATTCTCTTTTGATAGTATTTGTAGATTTTCCTTTGGTTTAGACCCTATGTGTCTAGAAACATCCTTACCCATGTCGGATTTTGCTCTTTCTTTTGATTTAGCATCAAAATTATCAGCTGAAAGAGCCATGGTTGTGTCTCCAATAATTTGGAAGATCAAAAGATTTTTCAACATGGGTAGTGAAAAGGAGTTAAAAAAAAGTATTAGCATCATTAATATGTTAGCAAAAGTTGTTATAGATCAAAAGAGAAAATTAGGTTTTTCCCATCACAAAGATTTGTTGTCAAGATTCATGAGTACTACAATTCATGATGACATGTTTCTTAGAGACATAGTCATAAGTTTCTTATTGGCTGGTCGTGACACCGTGGCATCATCACTCACAAGTTTCTTTTGGTTATTAGCAAAAAATCCGGAAGTTGAGAAAGAGATTTTGTTAGAAGCTGAACAAGTAATTGGACCACGTGATGATGAAAAAAATAATTATGGTGTAACGAATTTTGAACAACTTAGGAAGTTGCATTATTTACAAGCTGCAGCACATGAAAGTATGAGACTTTATCCACCAATTCAATTTGATTCAAAGTTTTGTTTGGATGATGATGTTTTACCAGATGGTACAAAAGTGAAAAGTGGTACTAGGGTTACTTATCATCCTTATGCAATGGGTAGGTTGGAGGAATTGTGGGGTCCAGATTGTTCAGAGTTTAAGCCTCAAAGGTGGTTGAAAGATGGTGTGTTTCAACCTTCAAATCAATTCAAGTACCCTATTTTTCAAGCTGGGTTAAGGGTTTGTATTGGTAAAGAAATGGCTTTGATGGAACTTAAAAGTGTGGCAATTTCTTTGCTTAGAAAGTTTCATATTGAATTGGAAGATAATACAATGTTTCATGGTAACCCAAGATTCTCTCCTGGACTAACTGCCACTTTTGCTTTTGGTCTTCCTGTATTTGTTCGTCCTAGAGGAACCAACTGA

Protein sequence

>Msa0163710

MASLELNKQSIRSFAFEVVNNEIQHRLIPLLQNQNLDQVVFDLQDVFKRFSFDSICRFSFGLDPMCLETSLPMSDFALSFDLASKLSAERAMVVSPIIWKIKRFFNMGSEKELKKSISIINMLAKVVIDQKRKLGFSHHKDLLSRFMSTTIHDDMFLRDIVISFLLAGRDTVASSLTSFFWLLAKNPEVEKEILLEAEQVIGPRDDEKNNYGVTNFEQLRKLHYLQAAAHESMRLYPPIQFDSKFCLDDDVLPDGTKVKSGTRVTYHPYAMGRLEELWGPDCSEFKPQRWLKDGVFQPSNQFKYPIFQAGLRVCIGKEMALMELKSVAISLLRKFHIELEDNTMFHGNPRFSPGLTATFAFGLPVFVRPRGTN*