AlfalfaGEDB | Msa0164160

M. sativa cultivar zhongmu-4 / Msa0164160

KEGG: K00134
GO: GO:0000003 GO:0000302 GO:0003006 GO:0003674 GO:0003824 GO:0004365 GO:0005488 GO:0005507 GO:0005575 GO:0005576 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005739 GO:0005740 GO:0005773 GO:0005774 GO:0005777 GO:0005829 GO:0005886 GO:0005911 GO:0005975 GO:0005996 GO:0006006 GO:0006082 GO:0006090 GO:0006091 GO:0006094 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006970 GO:0006979 GO:0007275 GO:0008150 GO:0008152 GO:0008270 GO:0008886 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009266 GO:0009408 GO:0009506 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009636 GO:0009651 GO:0009743 GO:0009744 GO:0009791 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010154 GO:0016020 GO:0016051 GO:0016052 GO:0016053 GO:0016310 GO:0016491 GO:0016620 GO:0016903 GO:0017144 GO:0018130 GO:0019318 GO:0019319 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0022414 GO:0030054 GO:0030312 GO:0031090 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0032501 GO:0032502 GO:0032787 GO:0034285 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042221 GO:0042493 GO:0042542 GO:0042579 GO:0042742 GO:0042866 GO:0043167 GO:0043169 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043436 GO:0043891 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044428 GO:0044429 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046031 GO:0046034 GO:0046364 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046677 GO:0046686 GO:0046700 GO:0046872 GO:0046914 GO:0046939 GO:0048046 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050896 GO:0051186 GO:0051188 GO:0051704 GO:0051707 GO:0051775 GO:0055044 GO:0055086 GO:0055114 GO:0061458 GO:0070013 GO:0071704 GO:0071944 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:0098542 GO:0098588 GO:0098805 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576 GO:1901700
PFAM: PF00044.27

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0164160	sp\|P34922\|G3PC_PEA	86.111	72	8	1	5	76	13	82	3.34e-38	132

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0164160	A0A072UPF9	88.889	72	6	1	5	76	14	83	1.19e-37	135

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0164160	Msa0234270	0.817646	2.960222e-52	-8.615850e-47
Msa0164160	Msa0234320	0.803774	2.969747e-49	-8.615850e-47
Msa0164160	Msa0354680	0.877900	4.333568e-69	-8.615850e-47
Msa0164160	Msa0663800	0.930349	1.863473e-93	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0164160	MtrunA17_Chr4g0057861	88.889	72	6	1	5	76	14	83	1.72e-40	135
Msa0164160	MtrunA17_Chr6g0458861	74.648	71	16	1	5	75	12	80	1.32e-30	109
Msa0164160	MtrunA17_Chr3g0122971	79.545	44	7	1	5	48	15	56	6.58e-18	75.5
Msa0164160	MtrunA17_Chr2g0310421	52.113	71	31	2	5	75	91	158	2.14e-16	71.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0164160	AT3G04120.1	80.282	71	12	1	5	75	14	82	2.94e-34	119
Msa0164160	AT1G13440.2	78.873	71	13	1	5	75	14	82	8.87e-34	117
Msa0164160	AT1G13440.1	78.873	71	13	1	5	75	14	82	1.67e-33	117
Msa0164160	AT1G16300.1	54.930	71	29	2	5	75	93	160	4.43e-18	77.0
Msa0164160	AT1G79530.1	53.521	71	30	2	5	75	95	162	1.11e-17	75.9

Find 12 sgRNAs with CRISPR-Local

Find 164 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATGTGTGCAAAGACGGTAAT+TGG	0.320945	1_4:+43162392	None:intergenic
AAACGATGAACTAACTATAA+AGG	0.365642	1_4:-43162444	Msa0164160:CDS
AAGAATCGGACTCGGACGTT+TGG	0.399834	1_4:-43162698	Msa0164160:CDS
TTTCATCACCGCTAATTACA+TGG	0.472724	1_4:-43162620	Msa0164160:intron
TTTAAGTATGACAGTGTTCA+CGG	0.475837	1_4:-43162476	Msa0164160:CDS
CTGACAGGAGTTGGAAGAAT+CGG	0.476646	1_4:-43162712	Msa0164160:intron
TTGGCTTCTCATCGAAGAGA+AGG	0.541066	1_4:+43162411	None:intergenic
ATTATACATGTGTGCAAAGA+CGG	0.544343	1_4:+43162385	None:intergenic
TGGCTTCTCATCGAAGAGAA+GGG	0.552508	1_4:+43162412	None:intergenic
ATGACAGTGTTCACGGACAA+TGG	0.557529	1_4:-43162469	Msa0164160:CDS
ATGTAATTAGCGGTGATGAA+AGG	0.572192	1_4:+43162622	None:intergenic
GGAGTTGGAAGAATCGGACT+CGG	0.670901	1_4:-43162706	Msa0164160:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TAGACTAATTAAAATATTAA+TGG	+	chr1_4:43163633-43163652	None:intergenic	10.0%
!!	AATGAAAAAATTAACTAAGA+TGG	+	chr1_4:43162013-43162032	None:intergenic	15.0%
!!!	TCATTAATATCAAACATTTT+TGG	+	chr1_4:43162420-43162439	None:intergenic	15.0%
!!	TATATATTATGTTGAAAGTT+CGG	-	chr1_4:43162671-43162690	Msa0164160:CDS	15.0%
!!!	TCTATTGAATTTATGAATTT+TGG	-	chr1_4:43163070-43163089	Msa0164160:intron	15.0%
!!!	CTATTGAATTTATGAATTTT+GGG	-	chr1_4:43163071-43163090	Msa0164160:intron	15.0%
!!!	TATTGAATTTATGAATTTTG+GGG	-	chr1_4:43163072-43163091	Msa0164160:intron	15.0%
!!	GAAAATTAAACGAAAAATTA+AGG	+	chr1_4:43163611-43163630	None:intergenic	15.0%
!!!	ATTTATTTTTGTGAATTTTG+TGG	-	chr1_4:43163963-43163982	Msa0164160:intron	15.0%
!!!	TGTAGAATTAATTGTTTTTT+GGG	-	chr1_4:43164268-43164287	Msa0164160:intron	15.0%
!!!	ATTATTGGTTTATGATAAAA+TGG	-	chr1_4:43164291-43164310	Msa0164160:intron	15.0%
!!!	TTATTGGTTTATGATAAAAT+GGG	-	chr1_4:43164292-43164311	Msa0164160:intron	15.0%
!!!	TAATTTTGAACTATGTTTAT+AGG	-	chr1_4:43164737-43164756	Msa0164160:intron	15.0%
!!	AAAAAATTAACTAAGATGGA+AGG	+	chr1_4:43162009-43162028	None:intergenic	20.0%
!!!	ATTGATCACATTAATGTTTT+CGG	-	chr1_4:43162575-43162594	Msa0164160:intron	20.0%
!!	GAAAACAAATTAAAGGAAAA+AGG	+	chr1_4:43162987-43163006	None:intergenic	20.0%
!!	ATCAAGAGAAAACAAATTAA+AGG	+	chr1_4:43162994-43163013	None:intergenic	20.0%
!!!	ATTTGTTTTCTCTTGATTAT+TGG	-	chr1_4:43162997-43163016	Msa0164160:intron	20.0%
!!	AATAAATTCAATAGACTTGT+AGG	+	chr1_4:43163031-43163050	None:intergenic	20.0%
!!	TTAAACCTGTTTCATAAATT+AGG	-	chr1_4:43163233-43163252	Msa0164160:intron	20.0%
!!	AAATTAGGAACATGTTATAT+TGG	-	chr1_4:43163248-43163267	Msa0164160:intron	20.0%
!!!	TTTTACACTACAAGAAATAT+GGG	-	chr1_4:43163289-43163308	Msa0164160:intron	20.0%
!!	AAAACATATTTCAGTCAAAA+GGG	-	chr1_4:43163387-43163406	Msa0164160:intron	20.0%
!!!	ATTTGTTTTATTTTAACGTG+TGG	+	chr1_4:43163508-43163527	None:intergenic	20.0%
!!	AATTAAGGAAAATAATGAAG+AGG	+	chr1_4:43163596-43163615	None:intergenic	20.0%
!!!	ATTTTATACCATGTAATTAG+CGG	+	chr1_4:43163876-43163895	None:intergenic	20.0%
!!!	CTGTAGAATTAATTGTTTTT+TGG	-	chr1_4:43164267-43164286	Msa0164160:intron	20.0%
!!!	TAATTGTTTTTTGGGATTAT+TGG	-	chr1_4:43164276-43164295	Msa0164160:intron	20.0%
!!	TCTAAGAATTGAAATGCTTT+TGG	-	chr1_4:43161678-43161697	Msa0164160:intron	25.0%
!!	CAATTTGTTCAGACAATTTA+AGG	-	chr1_4:43162041-43162060	Msa0164160:intron	25.0%
!!	ATCATCATTGACATCAATTT+TGG	-	chr1_4:43162323-43162342	Msa0164160:intron	25.0%
!	TTTATGTACATCAACATTGT+TGG	+	chr1_4:43162359-43162378	None:intergenic	25.0%
!	GTACATAAAACAAATCAAAG+TGG	-	chr1_4:43162370-43162389	Msa0164160:intron	25.0%
!!	TTGATCACATTAATGTTTTC+GGG	-	chr1_4:43162576-43162595	Msa0164160:intron	25.0%
!!!	GCAAAAATTTTAGAAGTGTT+AGG	-	chr1_4:43162758-43162777	Msa0164160:intron	25.0%
!	TTTGATTATAGTCCTAACTT+TGG	+	chr1_4:43162848-43162867	None:intergenic	25.0%
!!	TGAAAATTTCATTCTCCTTT+TGG	-	chr1_4:43162945-43162964	Msa0164160:intron	25.0%
!	ATGTTCCTAATTTATGAAAC+AGG	+	chr1_4:43163241-43163260	None:intergenic	25.0%
!	TTTCTTGTAGTGTAAAACAA+AGG	+	chr1_4:43163286-43163305	None:intergenic	25.0%
!!	GTTTTACACTACAAGAAATA+TGG	-	chr1_4:43163288-43163307	Msa0164160:intron	25.0%
!	GAAAACATATTTCAGTCAAA+AGG	-	chr1_4:43163386-43163405	Msa0164160:intron	25.0%
!	AAACATATTTCAGTCAAAAG+GGG	-	chr1_4:43163388-43163407	Msa0164160:intron	25.0%
!	TTAAGAGAAAAGACTTGAAA+TGG	-	chr1_4:43163418-43163437	Msa0164160:intron	25.0%
!	TTATTGAGGAAATATTTCCT+CGG	-	chr1_4:43163470-43163489	Msa0164160:intron	25.0%
!	CTAAAACAAAGGAAAAAATC+AGG	+	chr1_4:43163662-43163681	None:intergenic	25.0%
!	AAACGATGAACTAACTATAA+AGG	-	chr1_4:43164041-43164060	Msa0164160:intron	25.0%
!	ATTTCGTTCTTCTTATCTTT+GGG	-	chr1_4:43164241-43164260	Msa0164160:intron	25.0%
!!!	AATGGGTCATTTTTATTTGA+TGG	-	chr1_4:43164309-43164328	Msa0164160:intron	25.0%
!!	TTTTTCACTCCAATATCAAA+CGG	+	chr1_4:43164353-43164372	None:intergenic	25.0%
!	TTTGATATTGGAGTGAAAAA+AGG	-	chr1_4:43164353-43164372	Msa0164160:intron	25.0%
!!	GAAACCAAAGTTGATAGAAA+GGG	+	chr1_4:43161886-43161905	None:intergenic	30.0%
!!	AGAAACCAAAGTTGATAGAA+AGG	+	chr1_4:43161887-43161906	None:intergenic	30.0%
	ATGTCATGTCACACTATATA+TGG	-	chr1_4:43162202-43162221	Msa0164160:intron	30.0%
	GTCATAGATATATCCCAATA+AGG	-	chr1_4:43162224-43162243	Msa0164160:intron	30.0%
	AATTAAGTGGTCACCTTATT+GGG	+	chr1_4:43162240-43162259	None:intergenic	30.0%
	AAATTAAGTGGTCACCTTAT+TGG	+	chr1_4:43162241-43162260	None:intergenic	30.0%
	GTGAGCATTATCAAATTAAG+TGG	+	chr1_4:43162253-43162272	None:intergenic	30.0%
	TGATGATACTAAACCTCAAT+CGG	+	chr1_4:43162310-43162329	None:intergenic	30.0%
!!!	CAACATTAGTCTTTTTGGAT+CGG	+	chr1_4:43162627-43162646	None:intergenic	30.0%
!!!	TGCATCAACATTAGTCTTTT+TGG	+	chr1_4:43162632-43162651	None:intergenic	30.0%
	GTCATGTATCATAGAGTTTA+AGG	-	chr1_4:43162699-43162718	Msa0164160:CDS	30.0%
!!!	TTTGGCTTGTATTTTCCTTT+CGG	+	chr1_4:43162830-43162849	None:intergenic	30.0%
	AGGACTATAATCAAATGCAA+AGG	-	chr1_4:43162853-43162872	Msa0164160:intron	30.0%
	GTATAAGCAATCACAAAAAC+AGG	-	chr1_4:43162877-43162896	Msa0164160:intron	30.0%
	GCATTTCAATATTATGTGCA+AGG	+	chr1_4:43162925-43162944	None:intergenic	30.0%
!!!	TTTCATTCTCCTTTTGGTTT+TGG	-	chr1_4:43162951-43162970	Msa0164160:intron	30.0%
	AAAAGCTTACCAAAACCAAA+AGG	+	chr1_4:43162963-43162982	None:intergenic	30.0%
!!!	GAATTTATGAATTTTGGGGT+TGG	-	chr1_4:43163076-43163095	Msa0164160:intron	30.0%
!	TTTTCCTACAAATATTTCCG+AGG	-	chr1_4:43163313-43163332	Msa0164160:intron	30.0%
	ATTTCCTCGGAAATATTTGT+AGG	+	chr1_4:43163320-43163339	None:intergenic	30.0%
	ATGAGATGAGATATGTATGT+TGG	-	chr1_4:43163526-43163545	Msa0164160:intron	30.0%
	TGAGATGAGATATGTATGTT+GGG	-	chr1_4:43163527-43163546	Msa0164160:intron	30.0%
!	TAATTTTGTCTGTAACTGAC+AGG	-	chr1_4:43163758-43163777	Msa0164160:intron	30.0%
	TTTAAGTATGACAGTGTTCA+CGG	-	chr1_4:43164009-43164028	Msa0164160:intron	30.0%
	ATTATACATGTGTGCAAAGA+CGG	+	chr1_4:43164103-43164122	None:intergenic	30.0%
	GATTTCGTTCTTCTTATCTT+TGG	-	chr1_4:43164240-43164259	Msa0164160:intron	30.0%
!	TTTTCACTCCAATATCAAAC+GGG	+	chr1_4:43164352-43164371	None:intergenic	30.0%
!!	AAAGTTTGAAGCCATGATTT+TGG	-	chr1_4:43164377-43164396	Msa0164160:intron	30.0%
!	TGCTCAACTTTGTTTCAATA+TGG	-	chr1_4:43164488-43164507	Msa0164160:intron	30.0%
	TTCTATGTGTGTTAGTGTAT+AGG	-	chr1_4:43164521-43164540	Msa0164160:intron	30.0%
	TCTATGTGTGTTAGTGTATA+GGG	-	chr1_4:43164522-43164541	Msa0164160:intron	30.0%
	AAGTTTAGATGGAAGTTTGA+TGG	-	chr1_4:43164621-43164640	Msa0164160:intron	30.0%
	AGTTTAGATGGAAGTTTGAT+GGG	-	chr1_4:43164622-43164641	Msa0164160:intron	30.0%
	GAACTATGTTTATAGGAACT+TGG	-	chr1_4:43164744-43164763	Msa0164160:intron	30.0%
!	AGAATTGAAATGCTTTTGGC+TGG	-	chr1_4:43161682-43161701	Msa0164160:intron	35.0%
!	CAATAACTTGTGTCGCATTA+TGG	+	chr1_4:43161726-43161745	None:intergenic	35.0%
	ATGGAAGGTCTGGAAAAAAA+AGG	+	chr1_4:43161994-43162013	None:intergenic	35.0%
	ATTAACTAAGATGGAAGGTC+TGG	+	chr1_4:43162004-43162023	None:intergenic	35.0%
	AGTCAACAATGCAACACAAT+AGG	-	chr1_4:43162149-43162168	Msa0164160:intron	35.0%
	TCTTCAATTATGACCGATTG+AGG	-	chr1_4:43162294-43162313	Msa0164160:intron	35.0%
	ATGTACATCAACATTGTTGG+AGG	+	chr1_4:43162356-43162375	None:intergenic	35.0%
	AAGTGTTAGGAATACACTCT+GGG	-	chr1_4:43162771-43162790	Msa0164160:intron	35.0%
	TTGCTATAATATACCGTCCA+AGG	-	chr1_4:43162794-43162813	Msa0164160:intron	35.0%
	GGAAAATACAAGCCAAAGTT+AGG	-	chr1_4:43162833-43162852	Msa0164160:intron	35.0%
!	ACTTTTGTCGGTAATTTCGT+CGG	+	chr1_4:43163360-43163379	None:intergenic	35.0%
	AATTACCGACAAAAGTTCGT+AGG	-	chr1_4:43163364-43163383	Msa0164160:intron	35.0%
!	GTTTTCCTACGAACTTTTGT+CGG	+	chr1_4:43163372-43163391	None:intergenic	35.0%
!	CAAGTCTTTTCTCTTAACGT+GGG	+	chr1_4:43163415-43163434	None:intergenic	35.0%
!	TCAAGTCTTTTCTCTTAACG+TGG	+	chr1_4:43163416-43163435	None:intergenic	35.0%
	AGAGAAAAGACTTGAAATGG+AGG	-	chr1_4:43163421-43163440	Msa0164160:intron	35.0%
	AAATATTTCCTCGGAAAGCA+TGG	-	chr1_4:43163479-43163498	Msa0164160:intron	35.0%
!	AATATTTCCTCGGAAAGCAT+GGG	-	chr1_4:43163480-43163499	Msa0164160:intron	35.0%
	GATCGAAGGAACTAAAACAA+AGG	+	chr1_4:43163673-43163692	None:intergenic	35.0%
	CTCTTCATTTCTGCATAGAT+CGG	-	chr1_4:43163694-43163713	Msa0164160:intron	35.0%
	ATGTAATTAGCGGTGATGAA+AGG	+	chr1_4:43163866-43163885	None:intergenic	35.0%
	TTTCATCACCGCTAATTACA+TGG	-	chr1_4:43163865-43163884	Msa0164160:intron	35.0%
	TTTCACTCCAATATCAAACG+GGG	+	chr1_4:43164351-43164370	None:intergenic	35.0%
!!	TCCATCTAAACTTTGAAGCA+GGG	+	chr1_4:43164614-43164633	None:intergenic	35.0%
!!	TTCCATCTAAACTTTGAAGC+AGG	+	chr1_4:43164615-43164634	None:intergenic	35.0%
	CATTTGATTCACACACTAGT+TGG	+	chr1_4:43164687-43164706	None:intergenic	35.0%
	CAACTAGTGTGTGAATCAAA+TGG	-	chr1_4:43164685-43164704	Msa0164160:intron	35.0%
	ATCAAAGACTATAAGAGCCA+AGG	-	chr1_4:43164806-43164825	Msa0164160:intron	35.0%
	GCTTCCCTTTCTATCAACTT+TGG	-	chr1_4:43161879-43161898	Msa0164160:intron	40.0%
!	GAAGTGTTAGGAATACACTC+TGG	-	chr1_4:43162770-43162789	Msa0164160:intron	40.0%
	CTATAATATACCGTCCAAGG+TGG	-	chr1_4:43162797-43162816	Msa0164160:intron	40.0%
!!	TATGCCCTTTCCTTGTTGTT+TGG	-	chr1_4:43163146-43163165	Msa0164160:intron	40.0%
	GCAACCAAACAACAAGGAAA+GGG	+	chr1_4:43163153-43163172	None:intergenic	40.0%
	AGCAACCAAACAACAAGGAA+AGG	+	chr1_4:43163154-43163173	None:intergenic	40.0%
	TTCTCAGCAACCAAACAACA+AGG	+	chr1_4:43163159-43163178	None:intergenic	40.0%
	TATTTCCGAGGAAATTACCG+AGG	-	chr1_4:43163325-43163344	Msa0164160:intron	40.0%
!	ATATTTCCTCGGAAAGCATG+GGG	-	chr1_4:43163481-43163500	Msa0164160:intron	40.0%
	CAGAAATGAAGAGAGATCGA+AGG	+	chr1_4:43163687-43163706	None:intergenic	40.0%
	ATGTGTGCAAAGACGGTAAT+TGG	+	chr1_4:43164096-43164115	None:intergenic	40.0%
	GATCAATCCCCGTTTGATAT+TGG	-	chr1_4:43164341-43164360	Msa0164160:intron	40.0%
!!	GTTTGAAGCCATGATTTTGG+TGG	-	chr1_4:43164380-43164399	Msa0164160:intron	40.0%
	GGCTTGAGATAGATAGACAA+TGG	+	chr1_4:43164408-43164427	None:intergenic	40.0%
	TGAGAGTAGAACACGAATCA+TGG	+	chr1_4:43164429-43164448	None:intergenic	40.0%
	ACCCTGCTTCAAAGTTTAGA+TGG	-	chr1_4:43164610-43164629	Msa0164160:intron	40.0%
	TAGGAACTTGGATCAACTTG+AGG	-	chr1_4:43164756-43164775	Msa0164160:intron	40.0%
!!	AATGGATTGTGAAGATGCCT+TGG	+	chr1_4:43164826-43164845	None:intergenic	40.0%
	AGATAACCTAGTTGCAGCAA+TGG	+	chr1_4:43164844-43164863	None:intergenic	40.0%
	CGTTTCCTCGGTAATTTCCT+CGG	+	chr1_4:43163333-43163352	None:intergenic	45.0%
	CGAGGAAATTACCGAGGAAA+CGG	-	chr1_4:43163331-43163350	Msa0164160:intron	45.0%
	GAGGAAATTACCGAGGAAAC+GGG	-	chr1_4:43163332-43163351	Msa0164160:intron	45.0%
	AAGACTTGAAATGGAGGACG+TGG	-	chr1_4:43163427-43163446	Msa0164160:intron	45.0%
	TGTCTGTAACTGACAGGAGT+TGG	-	chr1_4:43163764-43163783	Msa0164160:intron	45.0%
	CTGACAGGAGTTGGAAGAAT+CGG	-	chr1_4:43163773-43163792	Msa0164160:intron	45.0%
	ATGACAGTGTTCACGGACAA+TGG	-	chr1_4:43164016-43164035	Msa0164160:intron	45.0%
	TGGCTTCTCATCGAAGAGAA+GGG	+	chr1_4:43164076-43164095	None:intergenic	45.0%
	TTGGCTTCTCATCGAAGAGA+AGG	+	chr1_4:43164077-43164096	None:intergenic	45.0%
	CAATGGCACCACCAAAATCA+TGG	+	chr1_4:43164391-43164410	None:intergenic	45.0%
	TCGTGTTCTACTCTCAAGTC+TGG	-	chr1_4:43164433-43164452	Msa0164160:intron	45.0%
	TGTGTGTTAGTGTATAGGGC+TGG	-	chr1_4:43164526-43164545	Msa0164160:intron	45.0%
!	GGGCTGGAATTGTGTTTTCT+TGG	-	chr1_4:43164542-43164561	Msa0164160:intron	45.0%
!	TAGATGGAAGTTTGATGGGC+TGG	-	chr1_4:43164626-43164645	Msa0164160:intron	45.0%
!!	AGATGGAAGTTTGATGGGCT+GGG	-	chr1_4:43164627-43164646	Msa0164160:intron	45.0%
	CACAATCCATTGCTGCAACT+AGG	-	chr1_4:43164835-43164854	Msa0164160:intron	45.0%
	CTTTCGGAGAAACTCCACCT+TGG	+	chr1_4:43162814-43162833	None:intergenic	50.0%
	CAAGGTGGAGTTTCTCCGAA+AGG	-	chr1_4:43162812-43162831	Msa0164160:intron	50.0%
!	GTGGTTGGTTGGGGTTATTG+AGG	-	chr1_4:43163456-43163475	Msa0164160:intron	50.0%
!	TGAAGAGGTGCGACTACTAG+CGG	+	chr1_4:43163581-43163600	None:intergenic	50.0%
	GGAGTTGGAAGAATCGGACT+CGG	-	chr1_4:43163779-43163798	Msa0164160:intron	50.0%
	AAGAATCGGACTCGGACGTT+TGG	-	chr1_4:43163787-43163806	Msa0164160:intron	50.0%
	CGGAGAAACTCCACCTTGGA+CGG	+	chr1_4:43162810-43162829	None:intergenic	55.0%
	TTCGTCGGTACCCGTTTCCT+CGG	+	chr1_4:43163345-43163364	None:intergenic	55.0%
!	GTGGCGTGTTGTGGTTGGTT+GGG	-	chr1_4:43163446-43163465	Msa0164160:intron	55.0%
!	TGGCGTGTTGTGGTTGGTTG+GGG	-	chr1_4:43163447-43163466	Msa0164160:intron	55.0%
	ATGGAGGACGTGGCGTGTTG+TGG	-	chr1_4:43163437-43163456	Msa0164160:intron	60.0%
	AGGACGTGGCGTGTTGTGGT+TGG	-	chr1_4:43163441-43163460	Msa0164160:intron	60.0%
	CGTGGCGTGTTGTGGTTGGT+TGG	-	chr1_4:43163445-43163464	Msa0164160:intron	60.0%
	TGTGGACCCCATGCTTTCCG+AGG	+	chr1_4:43163490-43163509	None:intergenic	60.0%
	TGCGACTACTAGCGGCGAGA+GGG	+	chr1_4:43163573-43163592	None:intergenic	60.0%
	GCGACTACTAGCGGCGAGAG+GGG	+	chr1_4:43163572-43163591	None:intergenic	65.0%
	GTGCGACTACTAGCGGCGAG+AGG	+	chr1_4:43163574-43163593	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr1_4	gene	43161606	43164901	43161606	ID=Msa0164160;Name=Msa0164160
chr1_4	mRNA	43161606	43164901	43161606	ID=Msa0164160-mRNA-1;Parent=Msa0164160;Name=Msa0164160-mRNA-1;_AED=0.44;_eAED=0.46;_QI=0\|0.33\|0.25\|0.75\|0.33\|0.5\|4\|0\|79
chr1_4	exon	43164895	43164901	43164895	ID=Msa0164160-mRNA-1:exon:12442;Parent=Msa0164160-mRNA-1
chr1_4	exon	43162621	43162727	43162621	ID=Msa0164160-mRNA-1:exon:12441;Parent=Msa0164160-mRNA-1
chr1_4	exon	43162392	43162507	43162392	ID=Msa0164160-mRNA-1:exon:12440;Parent=Msa0164160-mRNA-1
chr1_4	exon	43161606	43161612	43161606	ID=Msa0164160-mRNA-1:exon:12439;Parent=Msa0164160-mRNA-1
chr1_4	CDS	43164895	43164901	43164895	ID=Msa0164160-mRNA-1:cds;Parent=Msa0164160-mRNA-1
chr1_4	CDS	43162621	43162727	43162621	ID=Msa0164160-mRNA-1:cds;Parent=Msa0164160-mRNA-1
chr1_4	CDS	43162392	43162507	43162392	ID=Msa0164160-mRNA-1:cds;Parent=Msa0164160-mRNA-1
chr1_4	CDS	43161606	43161612	43161606	ID=Msa0164160-mRNA-1:cds;Parent=Msa0164160-mRNA-1

Gene Sequence	>Msa0164160 ATGCAAAGAGTTGGAAGAATCGGACTCGGACGTTTGGTTGCTAGATTTGCTTTGAAGAGAGATGATGTTGAACTTGTTGCTGTTAACGATCCTTTCATCACCGCTAATTACATGACATACATGTTTAAGTATGACAGTGTTCACGGACAATGGAAAAACGATGAACTAACTATAAAGGACTCTAAAACCCTTCTCTTCGATGAGAAGCCAATTACCGTCTTTGCACACATAAGTATT
Protein sequence	>Msa0164160 MQRVGRIGLGRLVARFALKRDDVELVAVNDPFITANYMTYMFKYDSVHGQWKNDELTIKDSKTLLFDEKPITVFAHISI