AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0170590

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0170590 MtrunA17_Chr1g0188161 93.827 81 5 0 1 81 1 81 8.95e-43 134
Msa0170590 MtrunA17_Chr1g0188131 81.481 81 13 2 1 80 1 80 2.80e-33 110
Msa0170590 MtrunA17_Chr1g0184941 55.172 58 26 0 23 80 24 81 2.12e-19 75.5
Msa0170590 MtrunA17_Chr1g0148341 37.349 83 46 1 4 80 14 96 1.25e-13 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0170590 AT3G22942.1 74.545 55 14 0 26 80 18 72 7.86e-20 76.6
Msa0170590 AT3G63420.1 54.688 64 29 0 17 80 7 70 4.72e-17 69.3
Msa0170590 AT3G63420.2 54.688 64 29 0 17 80 7 70 4.72e-17 69.3
Msa0170590 AT3G63420.3 54.688 64 29 0 17 80 47 110 4.17e-16 68.2

Find 52 sgRNAs with CRISPR-Local

Find 185 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTGAACACTTGTCTTTAT+TGG 0.138711 1_4:+54785422 Msa0170590:five_prime_UTR
GGGTCAATCAATATCATTTC+TGG 0.152641 1_4:+54785443 Msa0170590:five_prime_UTR
AAACTATCCTGAAAAGTTTA+TGG 0.164858 1_4:-54788462 None:intergenic
CTTGAACACTTGTCTTTATT+GGG 0.265247 1_4:+54785423 Msa0170590:five_prime_UTR
TTTAGTTCAGGTCTGTGTTT+TGG 0.275164 1_4:-54785305 None:intergenic
ATCTGCAGCCTTGCGAATCT+TGG 0.292697 1_4:-54788295 None:intergenic
TCTGCAGCCTTGCGAATCTT+GGG 0.295792 1_4:-54788294 None:intergenic
CGAATACTTGTGAGACTGTT+TGG 0.299419 1_4:+54788385 Msa0170590:three_prime_UTR
GAATACATGCTGAACTTAAA+AGG 0.312374 1_4:+54785678 Msa0170590:CDS
AAGAGGGTTCAGTTTAGTTC+AGG 0.319187 1_4:-54785317 None:intergenic
AATACGCTGAAAACAACTTA+TGG 0.324350 1_4:-54788438 None:intergenic
TTGGTCCCTTGAATCGCTTA+TGG 0.331606 1_4:+54788252 Msa0170590:three_prime_UTR
TGGTCCCTTGAATCGCTTAT+GGG 0.359334 1_4:+54788253 Msa0170590:three_prime_UTR
CAAACAAACAAACACACTTC+TGG 0.372544 1_4:+54785355 Msa0170590:five_prime_UTR
TTCTGGTTTGACAGAACAAT+TGG 0.384254 1_4:+54788233 Msa0170590:three_prime_UTR
TAGACCAATCAGGTATATAA+AGG 0.391133 1_4:-54788580 None:intergenic
TTTATATACCTGATTGGTCT+AGG 0.403706 1_4:+54788582 Msa0170590:three_prime_UTR
TATTCCTTTATATACCTGAT+TGG 0.409643 1_4:+54788576 Msa0170590:three_prime_UTR
GAATACTTGTGAGACTGTTT+GGG 0.440792 1_4:+54788386 Msa0170590:three_prime_UTR
TTATGGGATCGGTGGTTTGA+AGG 0.442573 1_4:+54788269 Msa0170590:three_prime_UTR
TGTGAAAGATTCAGAGAAAG+AGG 0.445294 1_4:-54785334 None:intergenic
AAAATGAATGCAGGGAGAGC+TGG 0.449989 1_4:+54787913 Msa0170590:intron
GGTCAATCAATATCATTTCT+GGG 0.454364 1_4:+54785444 Msa0170590:five_prime_UTR
GTGATGGGTCATGTGATTCT+CGG 0.459142 1_4:-54785606 None:intergenic
GGTCATGTGATTCTCGGATT+CGG 0.459276 1_4:-54785600 None:intergenic
TGTAAATTCCTAGACCAATC+AGG 0.466719 1_4:-54788590 None:intergenic
TGAACTTAAAAGGTTGGAAC+AGG 0.469117 1_4:+54785688 Msa0170590:CDS
CCTTGAATCGCTTATGGGAT+CGG 0.474836 1_4:+54788258 Msa0170590:three_prime_UTR
CTGCAGCCTTGCGAATCTTG+GGG 0.477925 1_4:-54788293 None:intergenic
TACGATGGAAGCAAAGGATC+AGG 0.480893 1_4:-54788122 None:intergenic
ACAGGAAACAAGGTACTTAG+AGG 0.494385 1_4:+54785706 Msa0170590:CDS
CCGATCCCATAAGCGATTCA+AGG 0.501198 1_4:-54788258 None:intergenic
ACATGCTGAACTTAAAAGGT+TGG 0.526371 1_4:+54785682 Msa0170590:CDS
CTGAGACTGAACCCGGTGAT+GGG 0.537392 1_4:-54785621 None:intergenic
GAATCACATGACCCATCACC+GGG 0.542597 1_4:+54785610 Msa0170590:CDS
AGAATCACATGACCCATCAC+CGG 0.550612 1_4:+54785609 Msa0170590:CDS
TTAGCATCAGAAAATGCAAT+CGG 0.557138 1_4:+54785571 Msa0170590:exon
CATCAGAAAATGCAATCGGA+TGG 0.564403 1_4:+54785575 Msa0170590:exon
TTTCTTCTTCAGATACTAGA+GGG 0.566543 1_4:+54785648 Msa0170590:CDS
ACAGAGACAGAAACAGAGAC+AGG 0.567091 1_4:-54785378 None:intergenic
GAACTGCAAAGTCTATGAAA+CGG 0.571007 1_4:-54785512 None:intergenic
CTTTCTTCTTCAGATACTAG+AGG 0.572274 1_4:+54785647 Msa0170590:CDS
ACTGAGACTGAACCCGGTGA+TGG 0.572686 1_4:-54785622 None:intergenic
TGCAGCCTTGCGAATCTTGG+GGG 0.580172 1_4:-54788292 None:intergenic
GTGAAAGATTCAGAGAAAGA+GGG 0.583475 1_4:-54785333 None:intergenic
CGATCCCATAAGCGATTCAA+GGG 0.587732 1_4:-54788257 None:intergenic
TGATGCTAAGATGGTAACAA+TGG 0.593641 1_4:-54785557 None:intergenic
TGAATCGCTTATGGGATCGG+TGG 0.603469 1_4:+54788261 Msa0170590:three_prime_UTR
ATCAGAAAATGCAATCGGAT+GGG 0.609307 1_4:+54785576 Msa0170590:exon
AAAGAGACTGAGACTGAACC+CGG 0.616487 1_4:-54785628 None:intergenic
AAAGGTTGGAACAGGAAACA+AGG 0.637890 1_4:+54785696 Msa0170590:CDS
AGTCTCACAAGTATTCGTGT+CGG 0.712449 1_4:-54788379 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTATGTGATTTTTTATTT+TGG + chr1_4:54787046-54787065 Msa0170590:intron 10.0%
!!! ATAGAAACAAAAGTATAAAA+TGG - chr1_4:54786402-54786421 None:intergenic 15.0%
!!! TTTTGTAGAAATTTTTCAAA+AGG + chr1_4:54786483-54786502 Msa0170590:intron 15.0%
!! TAACTGAATTAATATACTAT+TGG - chr1_4:54786696-54786715 None:intergenic 15.0%
!!! TTTTTATTTTGGAAATTTGT+AGG + chr1_4:54787057-54787076 Msa0170590:intron 15.0%
!! CTATAGACAAAAAAATATTA+AGG - chr1_4:54787123-54787142 None:intergenic 15.0%
!!! CTTAATATTTTTTTGTCTAT+AGG + chr1_4:54787121-54787140 Msa0170590:intron 15.0%
!!! ATTCTTATTTCTTACTAATA+AGG - chr1_4:54787681-54787700 None:intergenic 15.0%
!! ACATAATAGTTAACTTAAAA+AGG - chr1_4:54787731-54787750 None:intergenic 15.0%
!! GCTTAAAATTAATACTAAAA+TGG + chr1_4:54787983-54788002 Msa0170590:intron 15.0%
!! AATTCAAAGTTAAACTAATA+TGG - chr1_4:54788053-54788072 None:intergenic 15.0%
!!! TTTGAAGTTTTGTTCTTTAT+TGG + chr1_4:54785758-54785777 Msa0170590:intron 20.0%
!!! TTGAAGTTTTGTTCTTTATT+GGG + chr1_4:54785759-54785778 Msa0170590:intron 20.0%
!!! ATTTTATATGAGTATGGTTT+AGG + chr1_4:54785800-54785819 Msa0170590:intron 20.0%
!! TTGAGTGTAATTATATATGT+CGG + chr1_4:54785981-54786000 Msa0170590:intron 20.0%
!! TTTGATATTACTATTACCTA+AGG + chr1_4:54786079-54786098 Msa0170590:intron 20.0%
!!! TTGTATTTGTATTGACAAAT+TGG - chr1_4:54786335-54786354 None:intergenic 20.0%
!! AAAATTTCTACAAAATGAAC+AGG - chr1_4:54786478-54786497 None:intergenic 20.0%
!! ACTTTCTTAAGATATATTTC+TGG - chr1_4:54786571-54786590 None:intergenic 20.0%
!! AGTTAAGAAGATATATGTAA+AGG - chr1_4:54786726-54786745 None:intergenic 20.0%
!!! ATTTTCTCAAGAAAATTTAC+TGG + chr1_4:54787306-54787325 Msa0170590:intron 20.0%
!!! TTATCATGCTTCTATTTTTA+TGG - chr1_4:54787544-54787563 None:intergenic 20.0%
!! GAAGCATGATAAAAATAATT+TGG + chr1_4:54787552-54787571 Msa0170590:intron 20.0%
!!! ACTTCTGTAATGAAATTTTT+TGG + chr1_4:54787805-54787824 Msa0170590:intron 20.0%
!!! TAACTTTTCTTCTAAGATTA+TGG + chr1_4:54787859-54787878 Msa0170590:intron 20.0%
!!! TAACTAATGATACAATTTTC+TGG + chr1_4:54788216-54788235 Msa0170590:three_prime_UTR 20.0%
!!! AATAACTACTCAGTTTTATT+TGG + chr1_4:54788333-54788352 Msa0170590:three_prime_UTR 20.0%
! CATCTTTCTTTCCATAAAAA+TGG + chr1_4:54785468-54785487 Msa0170590:five_prime_UTR 25.0%
! AAGAACAAAACTTCAAAACA+TGG - chr1_4:54785755-54785774 None:intergenic 25.0%
!! TTGGGTAGTTTTTATATCAA+AGG + chr1_4:54785777-54785796 Msa0170590:intron 25.0%
!! CAAAGGATTTTATATGAGTA+TGG + chr1_4:54785794-54785813 Msa0170590:intron 25.0%
!! GTTTAATCTTTTGATCATGT+TGG + chr1_4:54785842-54785861 Msa0170590:intron 25.0%
! TGGATAGTAAATTATGAGTA+TGG + chr1_4:54786022-54786041 Msa0170590:intron 25.0%
! GTAAATTATGAGTATGGTTT+AGG + chr1_4:54786028-54786047 Msa0170590:intron 25.0%
! GTTTAAAGATGTATGAACAT+TGG + chr1_4:54786207-54786226 Msa0170590:intron 25.0%
!! TTACTATGATTGATGATTTG+TGG + chr1_4:54786287-54786306 Msa0170590:intron 25.0%
! CAATACAAATACAACTATAC+AGG + chr1_4:54786341-54786360 Msa0170590:intron 25.0%
! ATGACCTAAATGCAAATAAA+TGG - chr1_4:54786510-54786529 None:intergenic 25.0%
! TAAGAAGATATATGTAAAGG+CGG - chr1_4:54786723-54786742 None:intergenic 25.0%
! ACATATATCTTCTTAACTAC+TGG + chr1_4:54786728-54786747 Msa0170590:intron 25.0%
! AAATCATTCTTTCTATAGTC+TGG + chr1_4:54786905-54786924 Msa0170590:intron 25.0%
!!! TTATTTTGGAAATTTGTAGG+TGG + chr1_4:54787060-54787079 Msa0170590:intron 25.0%
!! TTTTACCAAATGTTTGTTTG+TGG + chr1_4:54787083-54787102 Msa0170590:intron 25.0%
! GAAAATAACGTTATGGAAAA+AGG - chr1_4:54787292-54787311 None:intergenic 25.0%
! TTCTTGAGAAAATAACGTTA+TGG - chr1_4:54787299-54787318 None:intergenic 25.0%
! TTAAAGTCCTTTCAATCTTA+TGG + chr1_4:54787422-54787441 Msa0170590:intron 25.0%
!!! TTATGGCATCTCTTTTTTTA+AGG + chr1_4:54787439-54787458 Msa0170590:intron 25.0%
! TGCACACATAAGTTAAAAAA+AGG + chr1_4:54787578-54787597 Msa0170590:intron 25.0%
!! TTTTCATTGTTCTGTCTTAT+TGG + chr1_4:54787756-54787775 Msa0170590:intron 25.0%
! TTCACTAAAAAAATGAATGC+AGG + chr1_4:54787904-54787923 Msa0170590:intron 25.0%
! TCACTAAAAAAATGAATGCA+GGG + chr1_4:54787905-54787924 Msa0170590:intron 25.0%
! AAACTATCCTGAAAAGTTTA+TGG - chr1_4:54788465-54788484 None:intergenic 25.0%
! TATTCCTTTATATACCTGAT+TGG + chr1_4:54788576-54788595 Msa0170590:three_prime_UTR 25.0%
TCTTGAACACTTGTCTTTAT+TGG + chr1_4:54785422-54785441 Msa0170590:five_prime_UTR 30.0%
CTTGAACACTTGTCTTTATT+GGG + chr1_4:54785423-54785442 Msa0170590:five_prime_UTR 30.0%
GGTCAATCAATATCATTTCT+GGG + chr1_4:54785444-54785463 Msa0170590:five_prime_UTR 30.0%
!! GAACAAAGACTCCATTTTTA+TGG - chr1_4:54785482-54785501 None:intergenic 30.0%
TTAGCATCAGAAAATGCAAT+CGG + chr1_4:54785571-54785590 Msa0170590:exon 30.0%
TTTCTTCTTCAGATACTAGA+GGG + chr1_4:54785648-54785667 Msa0170590:CDS 30.0%
GAATACATGCTGAACTTAAA+AGG + chr1_4:54785678-54785697 Msa0170590:CDS 30.0%
TTTAGGAACTATGATGAGTA+TGG + chr1_4:54785817-54785836 Msa0170590:intron 30.0%
GTAATTATATATGTCGGTGT+CGG + chr1_4:54785987-54786006 Msa0170590:intron 30.0%
! TTTAGGAAGTATGATGAGTA+TGG + chr1_4:54786045-54786064 Msa0170590:intron 30.0%
ATCAATACATTGATCACCTT+AGG - chr1_4:54786098-54786117 None:intergenic 30.0%
!! TAACAAAGTGTGTTTTAAGC+TGG + chr1_4:54786175-54786194 Msa0170590:intron 30.0%
! TACAGGCTTGATGATTTAAA+TGG + chr1_4:54786358-54786377 Msa0170590:intron 30.0%
AGGTCCATTTATTTGCATTT+AGG + chr1_4:54786503-54786522 Msa0170590:intron 30.0%
GAATTTACTCAGACTTGATA+AGG - chr1_4:54786631-54786650 None:intergenic 30.0%
AAGAAGATATATGTAAAGGC+GGG - chr1_4:54786722-54786741 None:intergenic 30.0%
AGTGTGACTTTGAATCTTAT+TGG + chr1_4:54786802-54786821 Msa0170590:intron 30.0%
TTTGAATCTTATTGGAGTGA+GGG + chr1_4:54786810-54786829 Msa0170590:intron 30.0%
TAGTTGCACTATACTTATCT+GGG + chr1_4:54786849-54786868 Msa0170590:intron 30.0%
TAGTCTTCCTTAGTATAGTA+TGG + chr1_4:54787224-54787243 Msa0170590:intron 30.0%
AGTCTTCCTTAGTATAGTAT+GGG + chr1_4:54787225-54787244 Msa0170590:intron 30.0%
GTTGGCAATTTGATGTATTA+TGG + chr1_4:54787258-54787277 Msa0170590:intron 30.0%
CAAGAAAATTTACTGGAACT+TGG + chr1_4:54787313-54787332 Msa0170590:intron 30.0%
! CTAATTTGAAACAATGAGCA+TGG + chr1_4:54787502-54787521 Msa0170590:intron 30.0%
!! AATAAGGTTTTGTCAAATGC+TGG - chr1_4:54787665-54787684 None:intergenic 30.0%
AAAAGGAAAACTAAGTGTTC+AGG - chr1_4:54787714-54787733 None:intergenic 30.0%
!!! ATTTTTTGGAGCTTTTTCTG+TGG + chr1_4:54787819-54787838 Msa0170590:intron 30.0%
AATACGCTGAAAACAACTTA+TGG - chr1_4:54788441-54788460 None:intergenic 30.0%
! CGTATTTCCATAAACTTTTC+AGG + chr1_4:54788455-54788474 Msa0170590:three_prime_UTR 30.0%
TAGACCAATCAGGTATATAA+AGG - chr1_4:54788583-54788602 None:intergenic 30.0%
!! TTTAGTTCAGGTCTGTGTTT+TGG - chr1_4:54785308-54785327 None:intergenic 35.0%
GTGAAAGATTCAGAGAAAGA+GGG - chr1_4:54785336-54785355 None:intergenic 35.0%
TGTGAAAGATTCAGAGAAAG+AGG - chr1_4:54785337-54785356 None:intergenic 35.0%
CAAACAAACAAACACACTTC+TGG + chr1_4:54785355-54785374 Msa0170590:five_prime_UTR 35.0%
GGGTCAATCAATATCATTTC+TGG + chr1_4:54785443-54785462 Msa0170590:five_prime_UTR 35.0%
GAACTGCAAAGTCTATGAAA+CGG - chr1_4:54785515-54785534 None:intergenic 35.0%
TGATGCTAAGATGGTAACAA+TGG - chr1_4:54785560-54785579 None:intergenic 35.0%
! TGCATTTTCTGATGCTAAGA+TGG - chr1_4:54785569-54785588 None:intergenic 35.0%
ATCAGAAAATGCAATCGGAT+GGG + chr1_4:54785576-54785595 Msa0170590:exon 35.0%
CTTTCTTCTTCAGATACTAG+AGG + chr1_4:54785647-54785666 Msa0170590:CDS 35.0%
ACATGCTGAACTTAAAAGGT+TGG + chr1_4:54785682-54785701 Msa0170590:CDS 35.0%
TGAACTTAAAAGGTTGGAAC+AGG + chr1_4:54785688-54785707 Msa0170590:CDS 35.0%
! TTTAGCTTCATTCTTCAGTG+AGG - chr1_4:54786265-54786284 None:intergenic 35.0%
TGAAGAAATGATGCGATTCT+AGG + chr1_4:54786594-54786613 Msa0170590:intron 35.0%
TTTACTCAGACTTGATAAGG+AGG - chr1_4:54786628-54786647 None:intergenic 35.0%
AAGGTTAACGGAGTTAGTTA+GGG - chr1_4:54786660-54786679 None:intergenic 35.0%
TAACTCCGTTAACCTTCTAT+TGG + chr1_4:54786664-54786683 Msa0170590:intron 35.0%
ACTAGCCAATAGAAGGTTAA+CGG - chr1_4:54786672-54786691 None:intergenic 35.0%
TATTGGAACTAGCCAATAGA+AGG - chr1_4:54786679-54786698 None:intergenic 35.0%
CTTTGAATCTTATTGGAGTG+AGG + chr1_4:54786809-54786828 Msa0170590:intron 35.0%
GTAGTTGCACTATACTTATC+TGG + chr1_4:54786848-54786867 Msa0170590:intron 35.0%
AGTTGCACTATACTTATCTG+GGG + chr1_4:54786850-54786869 Msa0170590:intron 35.0%
AGCTAATCCATCAAGAAAGA+TGG - chr1_4:54786972-54786991 None:intergenic 35.0%
GAGTGTATTTCGAAGTTTGT+AGG - chr1_4:54787011-54787030 None:intergenic 35.0%
GTGTACCACAAACAAACATT+TGG - chr1_4:54787091-54787110 None:intergenic 35.0%
TGTCTATAGGATAGAAGTTG+AGG + chr1_4:54787134-54787153 Msa0170590:intron 35.0%
AAGTTGAGGTTTGATGTCTT+AGG + chr1_4:54787148-54787167 Msa0170590:intron 35.0%
! TTGTTTACTAGCTTCAACGA+GGG - chr1_4:54787188-54787207 None:intergenic 35.0%
TGAACTCCCATACTATACTA+AGG - chr1_4:54787234-54787253 None:intergenic 35.0%
AGAGATGCCATAAGATTGAA+AGG - chr1_4:54787432-54787451 None:intergenic 35.0%
CTGGCAACAAAAATAGAAGA+TGG - chr1_4:54788077-54788096 None:intergenic 35.0%
!!! CACAAGAAAGTTTTGAGAAG+CGG - chr1_4:54788151-54788170 None:intergenic 35.0%
!! TTCTGGTTTGACAGAACAAT+TGG + chr1_4:54788233-54788252 Msa0170590:three_prime_UTR 35.0%
GAATACTTGTGAGACTGTTT+GGG + chr1_4:54788386-54788405 Msa0170590:three_prime_UTR 35.0%
! AAGAGGGTTCAGTTTAGTTC+AGG - chr1_4:54785320-54785339 None:intergenic 40.0%
CATCAGAAAATGCAATCGGA+TGG + chr1_4:54785575-54785594 Msa0170590:exon 40.0%
AAAGGTTGGAACAGGAAACA+AGG + chr1_4:54785696-54785715 Msa0170590:CDS 40.0%
! ACAGGAAACAAGGTACTTAG+AGG + chr1_4:54785706-54785725 Msa0170590:CDS 40.0%
TATTAGCACTGTCTACGTGT+CGG - chr1_4:54785959-54785978 None:intergenic 40.0%
AACAACAGTGGATGGATACT+CGG - chr1_4:54786235-54786254 None:intergenic 40.0%
CAGTTGTGAGATGAGAATTG+TGG + chr1_4:54786438-54786457 Msa0170590:intron 40.0%
GAAGGTTAACGGAGTTAGTT+AGG - chr1_4:54786661-54786680 None:intergenic 40.0%
TTCTATCAACAGTTCAGCCT+CGG + chr1_4:54786929-54786948 Msa0170590:intron 40.0%
! ACTGACTTATTGTATGTCCG+AGG - chr1_4:54786949-54786968 None:intergenic 40.0%
AGTCAGTCCATCTTTCTTGA+TGG + chr1_4:54786962-54786981 Msa0170590:intron 40.0%
AGTTTGTAGGACACATCTCA+AGG - chr1_4:54786998-54787017 None:intergenic 40.0%
! CTTGTTTACTAGCTTCAACG+AGG - chr1_4:54787189-54787208 None:intergenic 40.0%
AGTATGGGAGTTCACATAGT+TGG + chr1_4:54787240-54787259 Msa0170590:intron 40.0%
TGTCCTAAAGCAACACAGTA+TGG + chr1_4:54787369-54787388 Msa0170590:intron 40.0%
GTCCTAAAGCAACACAGTAT+GGG + chr1_4:54787370-54787389 Msa0170590:intron 40.0%
AGTATGGGACATACTACAGA+GGG + chr1_4:54787385-54787404 Msa0170590:intron 40.0%
!! TTTTCTGTGGATGCTGATTG+TGG + chr1_4:54787832-54787851 Msa0170590:intron 40.0%
GAGAGCTGGAAAAACTTGAA+AGG + chr1_4:54787927-54787946 Msa0170590:CDS 40.0%
GCTGGAAAAACTTGAAAGGA+TGG + chr1_4:54787931-54787950 Msa0170590:CDS 40.0%
TTGTACACTACTCAGCAATC+TGG - chr1_4:54788096-54788115 None:intergenic 40.0%
! TTTGAGAAGCGGTTTTACGA+TGG - chr1_4:54788140-54788159 None:intergenic 40.0%
AGTCTCACAAGTATTCGTGT+CGG - chr1_4:54788382-54788401 None:intergenic 40.0%
CGAATACTTGTGAGACTGTT+TGG + chr1_4:54788385-54788404 Msa0170590:three_prime_UTR 40.0%
ACAGAGACAGAAACAGAGAC+AGG - chr1_4:54785381-54785400 None:intergenic 45.0%
GGTCATGTGATTCTCGGATT+CGG - chr1_4:54785603-54785622 None:intergenic 45.0%
!! GTGATGGGTCATGTGATTCT+CGG - chr1_4:54785609-54785628 None:intergenic 45.0%
AGAATCACATGACCCATCAC+CGG + chr1_4:54785609-54785628 Msa0170590:CDS 45.0%
AAAGAGACTGAGACTGAACC+CGG - chr1_4:54785631-54785650 None:intergenic 45.0%
GTATCCGTGCTTCATAGCAT+AGG - chr1_4:54785913-54785932 None:intergenic 45.0%
!! TATATATGTCGGTGTCGGAC+AGG + chr1_4:54785992-54786011 Msa0170590:intron 45.0%
! ACGGAGTTAGTTAGGGTGTT+GGG - chr1_4:54786653-54786672 None:intergenic 45.0%
AACGGAGTTAGTTAGGGTGT+TGG - chr1_4:54786654-54786673 None:intergenic 45.0%
CATAGTGAGAGAGAGAGATG+TGG + chr1_4:54786882-54786901 Msa0170590:intron 45.0%
!! GTCCCATACTGTGTTGCTTT+AGG - chr1_4:54787375-54787394 None:intergenic 45.0%
CAGTATGGGACATACTACAG+AGG + chr1_4:54787384-54787403 Msa0170590:intron 45.0%
! ATGCTGGAGACAAACTTGCT+TGG - chr1_4:54787649-54787668 None:intergenic 45.0%
!! TCTGTCTTATTGGCTCTCTG+CGG + chr1_4:54787766-54787785 Msa0170590:intron 45.0%
AAAATGAATGCAGGGAGAGC+TGG + chr1_4:54787913-54787932 Msa0170590:intron 45.0%
TACGATGGAAGCAAAGGATC+AGG - chr1_4:54788125-54788144 None:intergenic 45.0%
! CGGTTTTACGATGGAAGCAA+AGG - chr1_4:54788131-54788150 None:intergenic 45.0%
TTGGTCCCTTGAATCGCTTA+TGG + chr1_4:54788252-54788271 Msa0170590:three_prime_UTR 45.0%
TGGTCCCTTGAATCGCTTAT+GGG + chr1_4:54788253-54788272 Msa0170590:three_prime_UTR 45.0%
CGATCCCATAAGCGATTCAA+GGG - chr1_4:54788260-54788279 None:intergenic 45.0%
CCTTGAATCGCTTATGGGAT+CGG + chr1_4:54788258-54788277 Msa0170590:three_prime_UTR 45.0%
TTATGGGATCGGTGGTTTGA+AGG + chr1_4:54788269-54788288 Msa0170590:three_prime_UTR 45.0%
GAATCACATGACCCATCACC+GGG + chr1_4:54785610-54785629 Msa0170590:CDS 50.0%
GCCTCCTATGCTATGAAGCA+CGG + chr1_4:54785906-54785925 Msa0170590:intron 50.0%
TCCGTGCTTCATAGCATAGG+AGG - chr1_4:54785910-54785929 None:intergenic 50.0%
GACTCGCTAACAACAGTGGA+TGG - chr1_4:54786243-54786262 None:intergenic 50.0%
TGAGGACTCGCTAACAACAG+TGG - chr1_4:54786247-54786266 None:intergenic 50.0%
GTGAGGGATGTGTTGAGACT+CGG + chr1_4:54786826-54786845 Msa0170590:intron 50.0%
CCGATCCCATAAGCGATTCA+AGG - chr1_4:54788261-54788280 None:intergenic 50.0%
TGAATCGCTTATGGGATCGG+TGG + chr1_4:54788261-54788280 Msa0170590:three_prime_UTR 50.0%
TCTGCAGCCTTGCGAATCTT+GGG - chr1_4:54788297-54788316 None:intergenic 50.0%
ATCTGCAGCCTTGCGAATCT+TGG - chr1_4:54788298-54788317 None:intergenic 50.0%
CTGAGACTGAACCCGGTGAT+GGG - chr1_4:54785624-54785643 None:intergenic 55.0%
ACTGAGACTGAACCCGGTGA+TGG - chr1_4:54785625-54785644 None:intergenic 55.0%
TGAAGCACGGATACCGACAC+CGG + chr1_4:54785919-54785938 Msa0170590:intron 55.0%
TGCAGCCTTGCGAATCTTGG+GGG - chr1_4:54788295-54788314 None:intergenic 55.0%
CTGCAGCCTTGCGAATCTTG+GGG - chr1_4:54788296-54788315 None:intergenic 55.0%
GTTCGTGTCGTGTCCGGTGT+CGG - chr1_4:54785935-54785954 None:intergenic 60.0%
! GTCGGTGTTCGTGTCGTGTC+CGG - chr1_4:54785941-54785960 None:intergenic 60.0%
!! GTCGGTGTCGGACAGGACTA+TGG + chr1_4:54785999-54786018 Msa0170590:intron 60.0%
! GGTGTCGGACAGGACTATGG+TGG + chr1_4:54786002-54786021 Msa0170590:intron 60.0%
CGACTTAGAGTCGCGACAGG+AGG - chr1_4:54786754-54786773 None:intergenic 60.0%
!! CGTCGACTTAGAGTCGCGAC+AGG - chr1_4:54786757-54786776 None:intergenic 60.0%
GAAGGCCCCCAAGATTCGCA+AGG + chr1_4:54788287-54788306 Msa0170590:three_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 54785256 54788602 54785256 ID=Msa0170590;Name=Msa0170590
chr1_4 mRNA 54785256 54788602 54785256 ID=Msa0170590-mRNA-1;Parent=Msa0170590;Name=Msa0170590-mRNA-1;_AED=0.03;_eAED=0.03;_QI=328|1|1|1|0|0|3|457|81
chr1_4 exon 54785256 54785727 54785256 ID=Msa0170590-mRNA-1:exon:16356;Parent=Msa0170590-mRNA-1
chr1_4 exon 54787926 54787978 54787926 ID=Msa0170590-mRNA-1:exon:16357;Parent=Msa0170590-mRNA-1
chr1_4 exon 54788097 54788602 54788097 ID=Msa0170590-mRNA-1:exon:16358;Parent=Msa0170590-mRNA-1
chr1_4 five_prime_UTR 54785256 54785583 54785256 ID=Msa0170590-mRNA-1:five_prime_utr;Parent=Msa0170590-mRNA-1
chr1_4 CDS 54785584 54785727 54785584 ID=Msa0170590-mRNA-1:cds;Parent=Msa0170590-mRNA-1
chr1_4 CDS 54787926 54787978 54787926 ID=Msa0170590-mRNA-1:cds;Parent=Msa0170590-mRNA-1
chr1_4 CDS 54788097 54788145 54788097 ID=Msa0170590-mRNA-1:cds;Parent=Msa0170590-mRNA-1
chr1_4 three_prime_UTR 54788146 54788602 54788146 ID=Msa0170590-mRNA-1:three_prime_utr;Parent=Msa0170590-mRNA-1
Gene Sequence

>Msa0170590

ATGCAATCGGATGGGTCCGAATCCGAGAATCACATGACCCATCACCGGGTTCAGTCTCAGTCTCTTTCTTCTTCAGATACTAGAGGGAAACATAGAATACATGCTGAACTTAAAAGGTTGGAACAGGAAACAAGGTACTTAGAGGGAGAGCTGGAAAAACTTGAAAGGATGGATAAAGCATCAACAACATGCAAAGAATTGCTGAGTAGTGTACAAACAAGACCTGATCCTTTGCTTCCATCGTAA
Protein sequence

>Msa0170590

MQSDGSESENHMTHHRVQSQSLSSSDTRGKHRIHAELKRLEQETRYLEGELEKLERMDKASTTCKELLSSVQTRPDPLLPS*