AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0078020

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0078020 MtrunA17_Chr1g0188161 93.827 81 5 0 1 81 1 81 1.22e-42 134
Msa0078020 MtrunA17_Chr1g0188131 81.481 81 13 2 1 80 1 80 8.56e-33 109
Msa0078020 MtrunA17_Chr1g0184941 55.172 58 26 0 23 80 24 81 5.36e-20 77.0
Msa0078020 MtrunA17_Chr1g0148341 38.554 83 45 1 4 80 14 96 1.90e-14 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0078020 AT3G22942.1 74.545 55 14 0 26 80 18 72 2.27e-19 75.5
Msa0078020 AT3G63420.1 53.125 64 30 0 17 80 7 70 5.21e-17 69.3
Msa0078020 AT3G63420.2 53.125 64 30 0 17 80 7 70 5.21e-17 69.3
Msa0078020 AT3G63420.3 53.125 64 30 0 17 80 47 110 5.52e-16 67.8

Find 50 sgRNAs with CRISPR-Local

Find 185 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTGAACACTTGTCTTTAT+TGG 0.138711 1_2:+55277593 Msa0078020:five_prime_UTR
GGGTCAATCAATATCATTTC+TGG 0.152641 1_2:+55277614 Msa0078020:five_prime_UTR
AAACTATCCTGAAAAGTTTA+TGG 0.164858 1_2:-55280634 None:intergenic
CTTGAACACTTGTCTTTATT+GGG 0.265247 1_2:+55277594 Msa0078020:five_prime_UTR
TTTAGTTCAGGTCTGTGTTT+TGG 0.275164 1_2:-55277476 None:intergenic
ATCTGCAGCCTTGCGAATCT+TGG 0.292697 1_2:-55280467 None:intergenic
TCTGCAGCCTTGCGAATCTT+GGG 0.295792 1_2:-55280466 None:intergenic
CGAATACTTGTGAGACTGTT+TGG 0.299419 1_2:+55280557 Msa0078020:three_prime_UTR
GAATACATGCTGAACTTAAA+AGG 0.312374 1_2:+55277850 Msa0078020:CDS
AAGAGGGTTCAGTTTAGTTC+AGG 0.319187 1_2:-55277488 None:intergenic
AATACGCTGAAAACAACTTA+TGG 0.324350 1_2:-55280610 None:intergenic
TTGGTCCCTTGAATCGCTTA+TGG 0.331606 1_2:+55280424 Msa0078020:three_prime_UTR
TGGTCCCTTGAATCGCTTAT+GGG 0.359334 1_2:+55280425 Msa0078020:three_prime_UTR
CAAACAAACAAACACACTTC+TGG 0.372544 1_2:+55277526 Msa0078020:five_prime_UTR
TTCTGGTTTGACAGAACAAT+TGG 0.384254 1_2:+55280405 Msa0078020:three_prime_UTR
TAGACCAATCAGGTATATAA+AGG 0.391133 1_2:-55280752 None:intergenic
TTTATATACCTGATTGGTCT+AGG 0.403706 1_2:+55280754 Msa0078020:three_prime_UTR
TATTCCTTTATATACCTGAT+TGG 0.409643 1_2:+55280748 Msa0078020:three_prime_UTR
GAATACTTGTGAGACTGTTT+GGG 0.440792 1_2:+55280558 Msa0078020:three_prime_UTR
TTATGGGATCGGTGGTTTGA+AGG 0.442573 1_2:+55280441 Msa0078020:three_prime_UTR
GGTCAATCAATATCATTTCT+GGG 0.454364 1_2:+55277615 Msa0078020:five_prime_UTR
GTGATGGGTCATGTGATTCT+CGG 0.459142 1_2:-55277778 None:intergenic
GGTCATGTGATTCTCGGATT+CGG 0.459276 1_2:-55277772 None:intergenic
TGTAAATTCCTAGACCAATC+AGG 0.466719 1_2:-55280762 None:intergenic
AAAATGAATGCAGGAAGAGC+TGG 0.468685 1_2:+55280085 Msa0078020:intron
TGAACTTAAAAGGTTGGAAC+AGG 0.469117 1_2:+55277860 Msa0078020:CDS
CCTTGAATCGCTTATGGGAT+CGG 0.474836 1_2:+55280430 Msa0078020:three_prime_UTR
CTGCAGCCTTGCGAATCTTG+GGG 0.477925 1_2:-55280465 None:intergenic
TACGATGGAAGAAAAGGATC+AGG 0.479360 1_2:-55280294 None:intergenic
ACAGGAAACAAGGTACTTAG+AGG 0.494385 1_2:+55277878 Msa0078020:CDS
CCGATCCCATAAGCGATTCA+AGG 0.501198 1_2:-55280430 None:intergenic
ACATGCTGAACTTAAAAGGT+TGG 0.526371 1_2:+55277854 Msa0078020:CDS
CTGAGACTGAACCCGGTGAT+GGG 0.537392 1_2:-55277793 None:intergenic
GAATCACATGACCCATCACC+GGG 0.542597 1_2:+55277782 Msa0078020:CDS
AGAATCACATGACCCATCAC+CGG 0.550612 1_2:+55277781 Msa0078020:CDS
TTAGCATCAGAAAATGCAAT+CGG 0.557138 1_2:+55277743 Msa0078020:exon
CATCAGAAAATGCAATCGGA+TGG 0.564403 1_2:+55277747 Msa0078020:exon
TTTCTTCTTCAGATACTAGA+GGG 0.566543 1_2:+55277820 Msa0078020:CDS
ACAGAGACAGAAACAGAGAC+AGG 0.567091 1_2:-55277549 None:intergenic
GAACTGCAAAGTCTATGAAA+CGG 0.571007 1_2:-55277683 None:intergenic
CTTTCTTCTTCAGATACTAG+AGG 0.572274 1_2:+55277819 Msa0078020:CDS
ACTGAGACTGAACCCGGTGA+TGG 0.572686 1_2:-55277794 None:intergenic
TGCAGCCTTGCGAATCTTGG+GGG 0.580172 1_2:-55280464 None:intergenic
CGATCCCATAAGCGATTCAA+GGG 0.587732 1_2:-55280429 None:intergenic
TGATGCTAAGATGGTAACAA+TGG 0.593641 1_2:-55277729 None:intergenic
TGAATCGCTTATGGGATCGG+TGG 0.603469 1_2:+55280433 Msa0078020:three_prime_UTR
ATCAGAAAATGCAATCGGAT+GGG 0.609307 1_2:+55277748 Msa0078020:exon
AAAGAGACTGAGACTGAACC+CGG 0.616487 1_2:-55277800 None:intergenic
AAAGGTTGGAACAGGAAACA+AGG 0.637890 1_2:+55277868 Msa0078020:CDS
AGTCTCACAAGTATTCGTGT+CGG 0.712449 1_2:-55280551 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTATGTGATTTTTTATTT+TGG + chr1_2:55279218-55279237 Msa0078020:intron 10.0%
!!! ATAGAAACAAAAGTATAAAA+TGG - chr1_2:55278574-55278593 None:intergenic 15.0%
!!! TTTTGTAGAAATTTTTCAAA+AGG + chr1_2:55278655-55278674 Msa0078020:intron 15.0%
!! TAACTGAATTAATATACTAT+TGG - chr1_2:55278868-55278887 None:intergenic 15.0%
!!! TTTTTATTTTGGAAATTTGT+AGG + chr1_2:55279229-55279248 Msa0078020:intron 15.0%
!! CTATAGACAAAAAAATATTA+AGG - chr1_2:55279295-55279314 None:intergenic 15.0%
!!! CTTAATATTTTTTTGTCTAT+AGG + chr1_2:55279293-55279312 Msa0078020:intron 15.0%
!!! ATTCTTATTTCTTACTAATA+AGG - chr1_2:55279853-55279872 None:intergenic 15.0%
!! ACATAATAGTTAACTTAAAA+AGG - chr1_2:55279903-55279922 None:intergenic 15.0%
!! GCTTAAAATTAATACTAAAA+TGG + chr1_2:55280155-55280174 Msa0078020:intron 15.0%
!! AATTCAAAGTTAAACTAATA+TGG - chr1_2:55280225-55280244 None:intergenic 15.0%
!!! TTTGAAGTTTTGTTCTTTAT+TGG + chr1_2:55277930-55277949 Msa0078020:intron 20.0%
!!! TTGAAGTTTTGTTCTTTATT+GGG + chr1_2:55277931-55277950 Msa0078020:intron 20.0%
!!! ATTTTATATGAGTATGGTTT+AGG + chr1_2:55277972-55277991 Msa0078020:intron 20.0%
!! TTGAGTGTAATTATATATGT+CGG + chr1_2:55278153-55278172 Msa0078020:intron 20.0%
!! TTTGATATTACTATTACCTA+AGG + chr1_2:55278251-55278270 Msa0078020:intron 20.0%
!!! TTGTATTTGTATTGACAAAT+TGG - chr1_2:55278507-55278526 None:intergenic 20.0%
!! AAAATTTCTACAAAATGAAC+AGG - chr1_2:55278650-55278669 None:intergenic 20.0%
!! ACTTTCTTAAGATATATTTC+TGG - chr1_2:55278743-55278762 None:intergenic 20.0%
!! AGTTAAGAAGATATATGTAA+AGG - chr1_2:55278898-55278917 None:intergenic 20.0%
!!! ATTTTCTCAAGAAAATTTAC+TGG + chr1_2:55279478-55279497 Msa0078020:intron 20.0%
!!! TTATCATGCTTCTATTTTTA+TGG - chr1_2:55279716-55279735 None:intergenic 20.0%
!! GAAGCATGATAAAAATAATT+TGG + chr1_2:55279724-55279743 Msa0078020:intron 20.0%
!!! ACTTCTGTAATGAAATTTTT+TGG + chr1_2:55279977-55279996 Msa0078020:intron 20.0%
!!! TAACTAATGATACAATTTTC+TGG + chr1_2:55280388-55280407 Msa0078020:three_prime_UTR 20.0%
!!! AATAACTACTCAGTTTTATT+TGG + chr1_2:55280505-55280524 Msa0078020:three_prime_UTR 20.0%
! CATCTTTCTTTCCATAAAAA+TGG + chr1_2:55277639-55277658 Msa0078020:five_prime_UTR 25.0%
! AAGAACAAAACTTCAAAACA+TGG - chr1_2:55277927-55277946 None:intergenic 25.0%
!! CAAAGGATTTTATATGAGTA+TGG + chr1_2:55277966-55277985 Msa0078020:intron 25.0%
!! GTTTAATCTTTTGATCATGT+TGG + chr1_2:55278014-55278033 Msa0078020:intron 25.0%
! TGGATAGTAAATTATGAGTA+TGG + chr1_2:55278194-55278213 Msa0078020:intron 25.0%
! GTAAATTATGAGTATGGTTT+AGG + chr1_2:55278200-55278219 Msa0078020:intron 25.0%
! GTTTAAAGATGTATGAACAT+TGG + chr1_2:55278379-55278398 Msa0078020:intron 25.0%
!! TTACTATGATTGATGATTTG+TGG + chr1_2:55278459-55278478 Msa0078020:intron 25.0%
! CAATACAAATACAACTATAC+AGG + chr1_2:55278513-55278532 Msa0078020:intron 25.0%
! ATGACCTAAATGCAAATAAA+TGG - chr1_2:55278682-55278701 None:intergenic 25.0%
! TAAGAAGATATATGTAAAGG+CGG - chr1_2:55278895-55278914 None:intergenic 25.0%
! ACATATATCTTCTTAACTAC+TGG + chr1_2:55278900-55278919 Msa0078020:intron 25.0%
! TAGTTGTACTATACTTATCT+GGG + chr1_2:55279021-55279040 Msa0078020:intron 25.0%
! AAATCATTCTTTCTATAGTC+TGG + chr1_2:55279077-55279096 Msa0078020:intron 25.0%
!!! TTATTTTGGAAATTTGTAGG+TGG + chr1_2:55279232-55279251 Msa0078020:intron 25.0%
!! TTTTACCAAATGTTTGTTTG+TGG + chr1_2:55279255-55279274 Msa0078020:intron 25.0%
! GAAAATAACGTTATGGAAAA+AGG - chr1_2:55279464-55279483 None:intergenic 25.0%
! TTCTTGAGAAAATAACGTTA+TGG - chr1_2:55279471-55279490 None:intergenic 25.0%
! TTAAAGTCCTTTCAATCTTA+TGG + chr1_2:55279594-55279613 Msa0078020:intron 25.0%
!!! TTATGGCATCTCTTTTTTTA+AGG + chr1_2:55279611-55279630 Msa0078020:intron 25.0%
! TGCACACATAAGTTAAAAAA+AGG + chr1_2:55279750-55279769 Msa0078020:intron 25.0%
!! TTTTCATTGTTCTGTCTTAT+TGG + chr1_2:55279928-55279947 Msa0078020:intron 25.0%
!!! TAGCTTTTCTTCTAAGATTA+TGG + chr1_2:55280031-55280050 Msa0078020:intron 25.0%
! TTCACTAAAAAAATGAATGC+AGG + chr1_2:55280076-55280095 Msa0078020:intron 25.0%
! AAACTATCCTGAAAAGTTTA+TGG - chr1_2:55280637-55280656 None:intergenic 25.0%
! TATTCCTTTATATACCTGAT+TGG + chr1_2:55280748-55280767 Msa0078020:three_prime_UTR 25.0%
GTGAAAAATTCAGAGAAAGA+GGG - chr1_2:55277507-55277526 None:intergenic 30.0%
TGTGAAAAATTCAGAGAAAG+AGG - chr1_2:55277508-55277527 None:intergenic 30.0%
TCTTGAACACTTGTCTTTAT+TGG + chr1_2:55277593-55277612 Msa0078020:five_prime_UTR 30.0%
CTTGAACACTTGTCTTTATT+GGG + chr1_2:55277594-55277613 Msa0078020:five_prime_UTR 30.0%
GGTCAATCAATATCATTTCT+GGG + chr1_2:55277615-55277634 Msa0078020:five_prime_UTR 30.0%
!! GAACAAAGACTCCATTTTTA+TGG - chr1_2:55277653-55277672 None:intergenic 30.0%
TTAGCATCAGAAAATGCAAT+CGG + chr1_2:55277743-55277762 Msa0078020:exon 30.0%
TTTCTTCTTCAGATACTAGA+GGG + chr1_2:55277820-55277839 Msa0078020:CDS 30.0%
GAATACATGCTGAACTTAAA+AGG + chr1_2:55277850-55277869 Msa0078020:CDS 30.0%
!!! AAGTTTTGTTCTTTATTGGG+TGG + chr1_2:55277934-55277953 Msa0078020:intron 30.0%
! TTGGGTGGTTTTTATATCAA+AGG + chr1_2:55277949-55277968 Msa0078020:intron 30.0%
TTTAGGAACTATGATGAGTA+TGG + chr1_2:55277989-55278008 Msa0078020:intron 30.0%
GTAATTATATATGTCGGTGT+CGG + chr1_2:55278159-55278178 Msa0078020:intron 30.0%
! TTTAGGAAGTATGATGAGTA+TGG + chr1_2:55278217-55278236 Msa0078020:intron 30.0%
ATCAATACATTGATCACCTT+AGG - chr1_2:55278270-55278289 None:intergenic 30.0%
!!! TAACAAAGTGTGTTTTTAGC+TGG + chr1_2:55278347-55278366 Msa0078020:intron 30.0%
! TACAGGCTTGATGATTTAAA+TGG + chr1_2:55278530-55278549 Msa0078020:intron 30.0%
AGGTCCATTTATTTGCATTT+AGG + chr1_2:55278675-55278694 Msa0078020:intron 30.0%
GAATTTACTCAGACTTGATA+AGG - chr1_2:55278803-55278822 None:intergenic 30.0%
AAGAAGATATATGTAAAGGC+GGG - chr1_2:55278894-55278913 None:intergenic 30.0%
AGTGTGACTTTGAATCTTAT+TGG + chr1_2:55278974-55278993 Msa0078020:intron 30.0%
TTTGAATCTTATTGGAGTGA+GGG + chr1_2:55278982-55279001 Msa0078020:intron 30.0%
GTAGTTGTACTATACTTATC+TGG + chr1_2:55279020-55279039 Msa0078020:intron 30.0%
AGTTGTACTATACTTATCTG+GGG + chr1_2:55279022-55279041 Msa0078020:intron 30.0%
TAGTCTTCCTTAGTATAGTA+TGG + chr1_2:55279396-55279415 Msa0078020:intron 30.0%
AGTCTTCCTTAGTATAGTAT+GGG + chr1_2:55279397-55279416 Msa0078020:intron 30.0%
GTTGGCAATTTGATGTATTA+TGG + chr1_2:55279430-55279449 Msa0078020:intron 30.0%
CAAGAAAATTTACTGGAACT+TGG + chr1_2:55279485-55279504 Msa0078020:intron 30.0%
! CTAATTTGAAACAATGAGCA+TGG + chr1_2:55279674-55279693 Msa0078020:intron 30.0%
!! AATAAGGTTTTGTCAAATGC+TGG - chr1_2:55279837-55279856 None:intergenic 30.0%
AAAAGGAAAACTAAGTGTTC+AGG - chr1_2:55279886-55279905 None:intergenic 30.0%
!!! ATTTTTTGGAGCTTTTTCTG+TGG + chr1_2:55279991-55280010 Msa0078020:intron 30.0%
AATACGCTGAAAACAACTTA+TGG - chr1_2:55280613-55280632 None:intergenic 30.0%
! CGTATTTCCATAAACTTTTC+AGG + chr1_2:55280627-55280646 Msa0078020:three_prime_UTR 30.0%
TAGACCAATCAGGTATATAA+AGG - chr1_2:55280755-55280774 None:intergenic 30.0%
!! TTTAGTTCAGGTCTGTGTTT+TGG - chr1_2:55277479-55277498 None:intergenic 35.0%
CAAACAAACAAACACACTTC+TGG + chr1_2:55277526-55277545 Msa0078020:five_prime_UTR 35.0%
GGGTCAATCAATATCATTTC+TGG + chr1_2:55277614-55277633 Msa0078020:five_prime_UTR 35.0%
GAACTGCAAAGTCTATGAAA+CGG - chr1_2:55277686-55277705 None:intergenic 35.0%
TGATGCTAAGATGGTAACAA+TGG - chr1_2:55277732-55277751 None:intergenic 35.0%
! TGCATTTTCTGATGCTAAGA+TGG - chr1_2:55277741-55277760 None:intergenic 35.0%
ATCAGAAAATGCAATCGGAT+GGG + chr1_2:55277748-55277767 Msa0078020:exon 35.0%
CTTTCTTCTTCAGATACTAG+AGG + chr1_2:55277819-55277838 Msa0078020:CDS 35.0%
ACATGCTGAACTTAAAAGGT+TGG + chr1_2:55277854-55277873 Msa0078020:CDS 35.0%
TGAACTTAAAAGGTTGGAAC+AGG + chr1_2:55277860-55277879 Msa0078020:CDS 35.0%
! TTTAGCTTCATTCTTCAGTG+AGG - chr1_2:55278437-55278456 None:intergenic 35.0%
TGAAGAAATGATGCGATTCT+AGG + chr1_2:55278766-55278785 Msa0078020:intron 35.0%
TTTACTCAGACTTGATAAGG+AGG - chr1_2:55278800-55278819 None:intergenic 35.0%
AAGGTTAACGGAGTTAGTTA+GGG - chr1_2:55278832-55278851 None:intergenic 35.0%
TAACTCCGTTAACCTTCTAT+TGG + chr1_2:55278836-55278855 Msa0078020:intron 35.0%
ACTAGCCAATAGAAGGTTAA+CGG - chr1_2:55278844-55278863 None:intergenic 35.0%
TATTGGAACTAGCCAATAGA+AGG - chr1_2:55278851-55278870 None:intergenic 35.0%
CTTTGAATCTTATTGGAGTG+AGG + chr1_2:55278981-55279000 Msa0078020:intron 35.0%
AGCTAATCCATCAAGAAAGA+TGG - chr1_2:55279144-55279163 None:intergenic 35.0%
GAGTGTATTTCGAAGTTTGT+CGG - chr1_2:55279183-55279202 None:intergenic 35.0%
GTGTACCACAAACAAACATT+TGG - chr1_2:55279263-55279282 None:intergenic 35.0%
TGTCTATAGGATAGAAGTTG+AGG + chr1_2:55279306-55279325 Msa0078020:intron 35.0%
AAGTTGAGGTTTGATGTCTT+AGG + chr1_2:55279320-55279339 Msa0078020:intron 35.0%
! TTGTTTACTAGCTTCAACGA+GGG - chr1_2:55279360-55279379 None:intergenic 35.0%
TGAACTCCCATACTATACTA+AGG - chr1_2:55279406-55279425 None:intergenic 35.0%
AGAGATGCCATAAGATTGAA+AGG - chr1_2:55279604-55279623 None:intergenic 35.0%
AAGAGCTGGAAAAACTTGAA+AGG + chr1_2:55280099-55280118 Msa0078020:CDS 35.0%
CTGGCAACAAAAATAGAAGA+TGG - chr1_2:55280249-55280268 None:intergenic 35.0%
!!! CACAAGAAAGTTTTGAGAAG+CGG - chr1_2:55280323-55280342 None:intergenic 35.0%
!! TTCTGGTTTGACAGAACAAT+TGG + chr1_2:55280405-55280424 Msa0078020:three_prime_UTR 35.0%
GAATACTTGTGAGACTGTTT+GGG + chr1_2:55280558-55280577 Msa0078020:three_prime_UTR 35.0%
! AAGAGGGTTCAGTTTAGTTC+AGG - chr1_2:55277491-55277510 None:intergenic 40.0%
CATCAGAAAATGCAATCGGA+TGG + chr1_2:55277747-55277766 Msa0078020:exon 40.0%
AAAGGTTGGAACAGGAAACA+AGG + chr1_2:55277868-55277887 Msa0078020:CDS 40.0%
! ACAGGAAACAAGGTACTTAG+AGG + chr1_2:55277878-55277897 Msa0078020:CDS 40.0%
TATTAGCACTGTCTACGTGT+CGG - chr1_2:55278131-55278150 None:intergenic 40.0%
AACAACAGTGGATGGATACT+CGG - chr1_2:55278407-55278426 None:intergenic 40.0%
CAGTTGTGAGATGAGAATTG+TGG + chr1_2:55278610-55278629 Msa0078020:intron 40.0%
GAAGGTTAACGGAGTTAGTT+AGG - chr1_2:55278833-55278852 None:intergenic 40.0%
TTCTATCAACAGTTCAGCCT+CGG + chr1_2:55279101-55279120 Msa0078020:intron 40.0%
! ACTGACTTATTGTATGTCCG+AGG - chr1_2:55279121-55279140 None:intergenic 40.0%
AGTCAGTCCATCTTTCTTGA+TGG + chr1_2:55279134-55279153 Msa0078020:intron 40.0%
! CTTGTTTACTAGCTTCAACG+AGG - chr1_2:55279361-55279380 None:intergenic 40.0%
AGTATGGGAGTTCACATAGT+TGG + chr1_2:55279412-55279431 Msa0078020:intron 40.0%
TGTCCTAAAGCAACACAGTA+TGG + chr1_2:55279541-55279560 Msa0078020:intron 40.0%
GTCCTAAAGCAACACAGTAT+GGG + chr1_2:55279542-55279561 Msa0078020:intron 40.0%
AGTATGGGACATACTACAGA+GGG + chr1_2:55279557-55279576 Msa0078020:intron 40.0%
!! TTTTCTGTGGATGCTGATTG+TGG + chr1_2:55280004-55280023 Msa0078020:intron 40.0%
AAAATGAATGCAGGAAGAGC+TGG + chr1_2:55280085-55280104 Msa0078020:intron 40.0%
GCTGGAAAAACTTGAAAGGA+TGG + chr1_2:55280103-55280122 Msa0078020:CDS 40.0%
TTGTACACTACTCAGCAATC+TGG - chr1_2:55280268-55280287 None:intergenic 40.0%
TACGATGGAAGAAAAGGATC+AGG - chr1_2:55280297-55280316 None:intergenic 40.0%
! CGGTTTTACGATGGAAGAAA+AGG - chr1_2:55280303-55280322 None:intergenic 40.0%
! TTTGAGAAGCGGTTTTACGA+TGG - chr1_2:55280312-55280331 None:intergenic 40.0%
AGTCTCACAAGTATTCGTGT+CGG - chr1_2:55280554-55280573 None:intergenic 40.0%
CGAATACTTGTGAGACTGTT+TGG + chr1_2:55280557-55280576 Msa0078020:three_prime_UTR 40.0%
ACAGAGACAGAAACAGAGAC+AGG - chr1_2:55277552-55277571 None:intergenic 45.0%
GGTCATGTGATTCTCGGATT+CGG - chr1_2:55277775-55277794 None:intergenic 45.0%
!! GTGATGGGTCATGTGATTCT+CGG - chr1_2:55277781-55277800 None:intergenic 45.0%
AGAATCACATGACCCATCAC+CGG + chr1_2:55277781-55277800 Msa0078020:CDS 45.0%
AAAGAGACTGAGACTGAACC+CGG - chr1_2:55277803-55277822 None:intergenic 45.0%
GTATCCGTGCTTCATAGCAT+AGG - chr1_2:55278085-55278104 None:intergenic 45.0%
!! TATATATGTCGGTGTCGGAC+AGG + chr1_2:55278164-55278183 Msa0078020:intron 45.0%
! ACGGAGTTAGTTAGGGTGTT+GGG - chr1_2:55278825-55278844 None:intergenic 45.0%
AACGGAGTTAGTTAGGGTGT+TGG - chr1_2:55278826-55278845 None:intergenic 45.0%
CATAGTGAGAGAGAGAGATG+TGG + chr1_2:55279054-55279073 Msa0078020:intron 45.0%
AGTTTGTCGGACACATCTCA+AGG - chr1_2:55279170-55279189 None:intergenic 45.0%
!! GTCCCATACTGTGTTGCTTT+AGG - chr1_2:55279547-55279566 None:intergenic 45.0%
CAGTATGGGACATACTACAG+AGG + chr1_2:55279556-55279575 Msa0078020:intron 45.0%
! ATGCTGGAGACAAACTTGCT+TGG - chr1_2:55279821-55279840 None:intergenic 45.0%
!! TCTGTCTTATTGGCTCTCTG+CGG + chr1_2:55279938-55279957 Msa0078020:intron 45.0%
TTGGTCCCTTGAATCGCTTA+TGG + chr1_2:55280424-55280443 Msa0078020:three_prime_UTR 45.0%
TGGTCCCTTGAATCGCTTAT+GGG + chr1_2:55280425-55280444 Msa0078020:three_prime_UTR 45.0%
CGATCCCATAAGCGATTCAA+GGG - chr1_2:55280432-55280451 None:intergenic 45.0%
CCTTGAATCGCTTATGGGAT+CGG + chr1_2:55280430-55280449 Msa0078020:three_prime_UTR 45.0%
TTATGGGATCGGTGGTTTGA+AGG + chr1_2:55280441-55280460 Msa0078020:three_prime_UTR 45.0%
GAATCACATGACCCATCACC+GGG + chr1_2:55277782-55277801 Msa0078020:CDS 50.0%
GCCTCCTATGCTATGAAGCA+CGG + chr1_2:55278078-55278097 Msa0078020:intron 50.0%
TCCGTGCTTCATAGCATAGG+AGG - chr1_2:55278082-55278101 None:intergenic 50.0%
GACTCGCTAACAACAGTGGA+TGG - chr1_2:55278415-55278434 None:intergenic 50.0%
TGAGGACTCGCTAACAACAG+TGG - chr1_2:55278419-55278438 None:intergenic 50.0%
GTGAGGGATGTGTTGAGACT+CGG + chr1_2:55278998-55279017 Msa0078020:intron 50.0%
CCGATCCCATAAGCGATTCA+AGG - chr1_2:55280433-55280452 None:intergenic 50.0%
TGAATCGCTTATGGGATCGG+TGG + chr1_2:55280433-55280452 Msa0078020:three_prime_UTR 50.0%
TCTGCAGCCTTGCGAATCTT+GGG - chr1_2:55280469-55280488 None:intergenic 50.0%
ATCTGCAGCCTTGCGAATCT+TGG - chr1_2:55280470-55280489 None:intergenic 50.0%
CTGAGACTGAACCCGGTGAT+GGG - chr1_2:55277796-55277815 None:intergenic 55.0%
ACTGAGACTGAACCCGGTGA+TGG - chr1_2:55277797-55277816 None:intergenic 55.0%
TGAAGCACGGATACCGACAC+CGG + chr1_2:55278091-55278110 Msa0078020:intron 55.0%
TGCAGCCTTGCGAATCTTGG+GGG - chr1_2:55280467-55280486 None:intergenic 55.0%
CTGCAGCCTTGCGAATCTTG+GGG - chr1_2:55280468-55280487 None:intergenic 55.0%
GTTCGTGTCGTGTCCGGTGT+CGG - chr1_2:55278107-55278126 None:intergenic 60.0%
! GTCGGTGTTCGTGTCGTGTC+CGG - chr1_2:55278113-55278132 None:intergenic 60.0%
!! GTCGGTGTCGGACAGGACTA+TGG + chr1_2:55278171-55278190 Msa0078020:intron 60.0%
! GGTGTCGGACAGGACTATGG+TGG + chr1_2:55278174-55278193 Msa0078020:intron 60.0%
CGACTTAGAGTCGCGACAGG+AGG - chr1_2:55278926-55278945 None:intergenic 60.0%
!! CGTCGACTTAGAGTCGCGAC+AGG - chr1_2:55278929-55278948 None:intergenic 60.0%
GAAGGCCCCCAAGATTCGCA+AGG + chr1_2:55280459-55280478 Msa0078020:three_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 55277427 55280774 55277427 ID=Msa0078020;Name=Msa0078020
chr1_2 mRNA 55277427 55280774 55277427 ID=Msa0078020-mRNA-1;Parent=Msa0078020;Name=Msa0078020-mRNA-1;_AED=0.03;_eAED=0.03;_QI=329|1|1|1|0|0.33|3|457|81
chr1_2 exon 55277427 55277899 55277427 ID=Msa0078020-mRNA-1:exon:17202;Parent=Msa0078020-mRNA-1
chr1_2 exon 55280098 55280150 55280098 ID=Msa0078020-mRNA-1:exon:17203;Parent=Msa0078020-mRNA-1
chr1_2 exon 55280269 55280774 55280269 ID=Msa0078020-mRNA-1:exon:17204;Parent=Msa0078020-mRNA-1
chr1_2 five_prime_UTR 55277427 55277755 55277427 ID=Msa0078020-mRNA-1:five_prime_utr;Parent=Msa0078020-mRNA-1
chr1_2 CDS 55277756 55277899 55277756 ID=Msa0078020-mRNA-1:cds;Parent=Msa0078020-mRNA-1
chr1_2 CDS 55280098 55280150 55280098 ID=Msa0078020-mRNA-1:cds;Parent=Msa0078020-mRNA-1
chr1_2 CDS 55280269 55280317 55280269 ID=Msa0078020-mRNA-1:cds;Parent=Msa0078020-mRNA-1
chr1_2 three_prime_UTR 55280318 55280774 55280318 ID=Msa0078020-mRNA-1:three_prime_utr;Parent=Msa0078020-mRNA-1
Gene Sequence

>Msa0078020

ATGCAATCGGATGGGTCCGAATCCGAGAATCACATGACCCATCACCGGGTTCAGTCTCAGTCTCTTTCTTCTTCAGATACTAGAGGGAAACATAGAATACATGCTGAACTTAAAAGGTTGGAACAGGAAACAAGGTACTTAGAGGAAGAGCTGGAAAAACTTGAAAGGATGGATAAAGCATCAACAACATGCAAAGAATTGCTGAGTAGTGTACAAACAAGACCTGATCCTTTTCTTCCATCGTAA
Protein sequence

>Msa0078020

MQSDGSESENHMTHHRVQSQSLSSSDTRGKHRIHAELKRLEQETRYLEEELEKLERMDKASTTCKELLSSVQTRPDPFLPS*