AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0174940

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0174940 MtrunA17_Chr1g0195781 98.634 366 5 0 1 366 1 366 0.0 756
Msa0174940 MtrunA17_Chr7g0245941 92.077 366 29 0 1 366 1 366 0.0 719
Msa0174940 MtrunA17_Chr8g0351631 64.481 366 122 1 1 366 83 440 9.56e-179 503
Msa0174940 MtrunA17_Chr8g0351611 64.499 369 121 4 1 366 1 362 1.72e-170 479
Msa0174940 MtrunA17_Chr8g0351581 69.453 311 94 1 56 366 21 330 3.67e-163 459
Msa0174940 MtrunA17_Chr8g0351641 63.462 312 109 1 55 366 1 307 3.26e-148 421
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0174940 AT2G45440.1 75.410 366 89 1 1 366 1 365 0.0 581
Msa0174940 AT3G60880.2 74.590 366 92 1 1 366 1 365 0.0 569
Msa0174940 AT3G60880.1 74.317 366 92 1 1 366 1 364 0.0 567

Find 113 sgRNAs with CRISPR-Local

Find 183 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGAATAGAATTTGCCAATT+TGG 0.151527 1_4:+60719465 Msa0174940:CDS
CCGCATTGGGAGGTGGAAAT+TGG 0.197311 1_4:-60718108 None:intergenic
ACCTATTTCCTTCACCAAAT+TGG 0.206485 1_4:-60719479 None:intergenic
GGTGGGAAAATCAAGGTTAT+TGG 0.223073 1_4:+60718869 Msa0174940:CDS
TGGTTAGTCGATATTAATTT+AGG 0.225709 1_4:+60719560 Msa0174940:exon
CTTGTACTGGTTACTTTGTT+TGG 0.243493 1_4:+60719719 Msa0174940:three_prime_UTR
GATTATTCAGGAACTCTTAC+TGG 0.297793 1_4:+60718060 Msa0174940:intron
ATCAAGGTTATTGGAAATAC+TGG 0.303578 1_4:+60718878 Msa0174940:CDS
GCCAATTTGGTGAAGGAAAT+AGG 0.310672 1_4:+60719478 Msa0174940:CDS
ATAGAAATTATGAAATGAAT+TGG 0.315357 1_4:-60717364 None:intergenic
GCGCACACAGTTAATTGTTT+TGG 0.323823 1_4:+60718848 Msa0174940:CDS
TGTTGGAACTGAAGATGTTC+AGG 0.332957 1_4:+60719513 Msa0174940:CDS
GTTGCAAGCAATCTGATTCC+TGG 0.342044 1_4:+60719265 Msa0174940:CDS
AATGGAACATTATTACATAT+TGG 0.350082 1_4:+60719874 Msa0174940:three_prime_UTR
TTGTTAGTTATTATTGAGTT+AGG 0.356978 1_4:+60719583 Msa0174940:three_prime_UTR
TAAGATCTTGTTTCTTGTAC+TGG 0.369891 1_4:+60719706 Msa0174940:three_prime_UTR
GTGTTCAAACCAATGGGATT+TGG 0.372649 1_4:-60719373 None:intergenic
AGCTCTTGCCTTTGATTGAC+TGG 0.378010 1_4:+60719338 Msa0174940:CDS
GCTACATTGAAGAGCTATAC+TGG 0.379000 1_4:+60717497 Msa0174940:CDS
ATCAATGCCATGATGCTAGA+TGG 0.385109 1_4:+60719215 Msa0174940:CDS
TTGCTCAACTTGGAGTTGTC+AGG 0.401383 1_4:+60719401 Msa0174940:CDS
ACAAATGGCAACCTGAAAAC+TGG 0.401923 1_4:-60719424 None:intergenic
TTTCATAATTTCTATCAGTT+AGG 0.402264 1_4:+60717372 Msa0174940:five_prime_UTR
CTTTCAAAGTGTGCTTTCCA+TGG 0.406034 1_4:+60719021 Msa0174940:CDS
AACACGGCTCTTGCTCAACT+TGG 0.407658 1_4:+60719391 Msa0174940:CDS
GGAAGGATGAACCGGAAAAC+CGG 0.409763 1_4:-60717537 None:intergenic
TCATCTCAAAACTCCGCATT+GGG 0.420171 1_4:-60718121 None:intergenic
GACTTATTGTATCCTTTCTC+CGG 0.423830 1_4:+60719616 Msa0174940:three_prime_UTR
CTTCATATAAACCCTTACTA+TGG 0.428403 1_4:+60718968 Msa0174940:CDS
GGAGTTGAAGGTGTTATTGT+TGG 0.429517 1_4:+60718770 Msa0174940:CDS
CATAGTAAGGGTTTATATGA+AGG 0.438848 1_4:-60718967 None:intergenic
TTACTATGGCAAAACCTCAT+TGG 0.439136 1_4:+60718982 Msa0174940:CDS
TATAATGTGCCTTCACGAAC+TGG 0.441534 1_4:+60719058 Msa0174940:CDS
GTTATTGTTGGTGGCACAAC+TGG 0.447799 1_4:+60718782 Msa0174940:CDS
AATTATGTATGTTGTTGTGC+TGG 0.460636 1_4:+60719664 Msa0174940:three_prime_UTR
CTTAGCTCAAAATACTTGCT+TGG 0.461095 1_4:+60719111 Msa0174940:CDS
GCTCAAAATACTTGCTTGGC+TGG 0.463469 1_4:+60719115 Msa0174940:CDS
TTCATCTCAAAACTCCGCAT+TGG 0.466411 1_4:-60718122 None:intergenic
GTGAAGGTCAATTAATGAGC+TGG 0.470889 1_4:+60718804 Msa0174940:CDS
AATAAAATCGTTGTGTGGAG+CGG 0.476639 1_4:+60719184 Msa0174940:CDS
ATGCGAGAACTCATGTTTGA+GGG 0.476788 1_4:+60719292 Msa0174940:CDS
AGATGGGGTTATGGAGCTAC+TGG 0.478069 1_4:+60719232 Msa0174940:CDS
TCAAGATCAAATCGACCATC+AGG 0.480309 1_4:-60718710 None:intergenic
GTTGAAGGTGTTATTGTTGG+TGG 0.483414 1_4:+60718773 Msa0174940:CDS
TAGAAATTATGAAATGAATT+GGG 0.484201 1_4:-60717363 None:intergenic
GGTGTCTGCTTCAGAGGATC+CGG 0.484695 1_4:+60717518 Msa0174940:CDS
CAAAACTCCGCATTGGGAGG+TGG 0.487292 1_4:-60718115 None:intergenic
CATGATGCTAGATGGGGTTA+TGG 0.493610 1_4:+60719223 Msa0174940:CDS
ATAAAATCGTTGTGTGGAGC+GGG 0.494740 1_4:+60719185 Msa0174940:CDS
AATATGTAATAATGTTCCAT+TGG 0.499133 1_4:-60719872 None:intergenic
TACTTGCTTGGCTGGTGTCA+AGG 0.500800 1_4:+60719123 Msa0174940:CDS
CAGATCGAGAGTGGAGTTGA+AGG 0.501168 1_4:+60718758 Msa0174940:CDS
AATGCGAGAACTCATGTTTG+AGG 0.508177 1_4:+60719291 Msa0174940:CDS
GTAAGATCGGAAGGATGAAC+CGG 0.510311 1_4:-60717545 None:intergenic
AACCAATGGGATTTGGCATG+TGG 0.510972 1_4:-60719366 None:intergenic
TATACTGGTGTCTGCTTCAG+AGG 0.514396 1_4:+60717512 Msa0174940:CDS
CACTTCTAAAATTGCATGAG+TGG 0.515874 1_4:-60719812 None:intergenic
ATTTGAAGTTCACTGAGGTA+TGG 0.516290 1_4:+60717475 Msa0174940:exon
GCAATTCATGCCACTGAGCA+AGG 0.519046 1_4:+60718920 Msa0174940:CDS
TTTCAAAGTGTGCTTTCCAT+GGG 0.520623 1_4:+60719022 Msa0174940:CDS
GATTTCACAGCTGCTTGTGT+AGG 0.522599 1_4:-60718086 None:intergenic
AAGAGCTTAGAATTCAATGT+AGG 0.522936 1_4:-60719322 None:intergenic
AAGAGCCGTGTTCAAACCAA+TGG 0.526094 1_4:-60719380 None:intergenic
TCTAATGGGAGAGGTACAAA+TGG 0.526400 1_4:-60719439 None:intergenic
AACTTCAAATCGAGCTGCTG+TGG 0.528627 1_4:-60717462 None:intergenic
AATCGACCATCAGGTAGGTA+TGG 0.528759 1_4:-60718701 None:intergenic
AGAATTTGCCAATTTGGTGA+AGG 0.533742 1_4:+60719471 Msa0174940:CDS
CAAAACCTCATTGGATGGTA+TGG 0.535451 1_4:+60718991 Msa0174940:CDS
GTGGAGAAGACGGTGGTTGG+CGG 0.540986 1_4:-60717443 None:intergenic
AGGAATGTGTGGGAAATGAC+AGG 0.543586 1_4:+60719143 Msa0174940:CDS
TCTTGAAGCATACGATGCCT+TGG 0.545550 1_4:+60718727 Msa0174940:CDS
TTCCACATGCCAAATCCCAT+TGG 0.545798 1_4:+60719364 Msa0174940:CDS
ACTTCTAAAATTGCATGAGT+GGG 0.548290 1_4:-60719811 None:intergenic
GGCTGGTGTCAAGGAATGTG+TGG 0.556357 1_4:+60719132 Msa0174940:CDS
ACAAGGTTAATACAATATGC+AGG 0.560106 1_4:-60719753 None:intergenic
TCGAGCTGCTGTGGAGAAGA+CGG 0.566913 1_4:-60717453 None:intergenic
CATAACCCCATCTAGCATCA+TGG 0.567567 1_4:-60719222 None:intergenic
TTGTTAGTAGTAAGATCGGA+AGG 0.567613 1_4:-60717554 None:intergenic
TCAATGCCATGATGCTAGAT+GGG 0.567969 1_4:+60719216 Msa0174940:CDS
TGGAAAAGCCAGTCAATCAA+AGG 0.572095 1_4:-60719346 None:intergenic
TATGGCAAAACCTCATTGGA+TGG 0.575275 1_4:+60718986 Msa0174940:CDS
ATATATGATCGTGGGTCCCA+TGG 0.576230 1_4:-60719038 None:intergenic
ATAGTAAGGGTTTATATGAA+GGG 0.577466 1_4:-60718966 None:intergenic
AGAGCCGTGTTCAAACCAAT+GGG 0.577523 1_4:-60719379 None:intergenic
GTAAATATGCAGATCGAGAG+TGG 0.578320 1_4:+60718749 Msa0174940:CDS
TGTTTGGCATATTTCAAACA+AGG 0.584439 1_4:-60719770 None:intergenic
TGAAGGTCAATTAATGAGCT+GGG 0.584644 1_4:+60718805 Msa0174940:CDS
TATTTACAGGACTTGTACAG+AGG 0.599967 1_4:+60718640 Msa0174940:intron
ATGAGTTCTCGCATTAAACC+AGG 0.602598 1_4:-60719283 None:intergenic
GCTGTGGAGAAGACGGTGGT+TGG 0.609598 1_4:-60717446 None:intergenic
AGAGGTAGACATCAAACCAA+TGG 0.618147 1_4:+60719856 Msa0174940:three_prime_UTR
AAATCCCATTGGTTTGAACA+CGG 0.620889 1_4:+60719375 Msa0174940:CDS
TCTCAAAACTCCGCATTGGG+AGG 0.623035 1_4:-60718118 None:intergenic
GGCACATTATATATGATCGT+GGG 0.623516 1_4:-60719046 None:intergenic
AGGCACATTATATATGATCG+TGG 0.625176 1_4:-60719047 None:intergenic
AAGGATACAATAAGTCTACA+AGG 0.625433 1_4:-60719609 None:intergenic
AAAACTCCATACCTACCTGA+TGG 0.627623 1_4:+60718695 Msa0174940:CDS
ATACTGGAAGTAACTCGACA+AGG 0.635535 1_4:+60718894 Msa0174940:CDS
TACTGGAAGTAACTCGACAA+GGG 0.637824 1_4:+60718895 Msa0174940:CDS
CTCGATCTGCATATTTACCA+AGG 0.639513 1_4:-60718744 None:intergenic
CTGATAATAAAATCGTTGTG+TGG 0.642995 1_4:+60719179 Msa0174940:CDS
GGTTTCTCAGTAAACCGCGA+AGG 0.644815 1_4:+60717393 Msa0174940:five_prime_UTR
ATGTCTTGACCAGTTCGTGA+AGG 0.650374 1_4:-60719067 None:intergenic
TATTCAGGAACTCTTACTGG+AGG 0.652852 1_4:+60718063 Msa0174940:intron
GTTGGTGGCACAACTGGTGA+AGG 0.663404 1_4:+60718788 Msa0174940:CDS
GCTGGTGTCAAGGAATGTGT+GGG 0.663954 1_4:+60719133 Msa0174940:CDS
AACCGCAAAACCTTGCTCAG+TGG 0.673625 1_4:-60718930 None:intergenic
GATCAAATCGACCATCAGGT+AGG 0.678861 1_4:-60718706 None:intergenic
AGCAACCATACCATCCAATG+AGG 0.685283 1_4:-60718996 None:intergenic
CCAATTTCCACCTCCCAATG+CGG 0.691962 1_4:+60718108 Msa0174940:CDS
CAATGCCATGATGCTAGATG+GGG 0.711874 1_4:+60719217 Msa0174940:CDS
GCTCGATTTGAAGTTCACTG+AGG 0.768591 1_4:+60717470 Msa0174940:five_prime_UTR
AGCTGCTGTGGAGAAGACGG+TGG 0.771661 1_4:-60717450 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAAACATTAAATAAAAAAAT+TGG - chr1_4:60718033-60718052 None:intergenic 10.0%
!! TAGAAATTATGAAATGAATT+GGG - chr1_4:60717366-60717385 None:intergenic 15.0%
!! ATAGAAATTATGAAATGAAT+TGG - chr1_4:60717367-60717386 None:intergenic 15.0%
!! AGATTTCAAAATAAAGAAAA+CGG - chr1_4:60717600-60717619 None:intergenic 15.0%
!! TTTCATAATTTCTATCAGTT+AGG + chr1_4:60717372-60717391 Msa0174940:five_prime_UTR 20.0%
!! AATGAATTCGATGAAATAAA+CGG + chr1_4:60717765-60717784 Msa0174940:intron 20.0%
!!! TATGATTTTAATGATGATGA+AGG + chr1_4:60717812-60717831 Msa0174940:intron 20.0%
!!! ATGATTTTAATGATGATGAA+GGG + chr1_4:60717813-60717832 Msa0174940:intron 20.0%
!!! GTTTTCATCTTTTTATTTTC+TGG + chr1_4:60718278-60718297 Msa0174940:intron 20.0%
!! TATTGTGATTTATGATGAAA+CGG + chr1_4:60718331-60718350 Msa0174940:intron 20.0%
!!! TTCTAAGGAGTATATTTAAA+AGG + chr1_4:60718526-60718545 Msa0174940:intron 20.0%
!! AAGCTATATATTGTATTTAC+AGG + chr1_4:60718627-60718646 Msa0174940:intron 20.0%
!!! ATTCTATTCTTTTCTCTAAT+GGG - chr1_4:60719456-60719475 None:intergenic 20.0%
!!! AATTCTATTCTTTTCTCTAA+TGG - chr1_4:60719457-60719476 None:intergenic 20.0%
!! TTGTTAGTTATTATTGAGTT+AGG + chr1_4:60719583-60719602 Msa0174940:three_prime_UTR 20.0%
!! AATATGTAATAATGTTCCAT+TGG - chr1_4:60719875-60719894 None:intergenic 20.0%
!! AATGGAACATTATTACATAT+TGG + chr1_4:60719874-60719893 Msa0174940:three_prime_UTR 20.0%
!! GTTTTTGTTAGTAGTAAGAT+CGG - chr1_4:60717561-60717580 None:intergenic 25.0%
!! TTGAGAGTATAATTTAGCTA+AGG + chr1_4:60717864-60717883 Msa0174940:intron 25.0%
!!! TTTTGAGATGAAAAATAGGT+TGG + chr1_4:60718133-60718152 Msa0174940:intron 25.0%
! AGTATCAAAACTAATGAATG+TGG - chr1_4:60718180-60718199 None:intergenic 25.0%
! ATTGTGATTTATGATGAAAC+GGG + chr1_4:60718332-60718351 Msa0174940:intron 25.0%
! GAAAAACAAATCCAAATTAG+TGG - chr1_4:60718387-60718406 None:intergenic 25.0%
! AATCTCAAATTGAATGATAG+AGG - chr1_4:60718409-60718428 None:intergenic 25.0%
!!! AGATTTTGATTTGACATACA+AGG + chr1_4:60718424-60718443 Msa0174940:intron 25.0%
!! TAAGGAGTATATTTAAAAGG+TGG + chr1_4:60718529-60718548 Msa0174940:intron 25.0%
!!! TGTTGCTTTTGTTTTAATCA+TGG + chr1_4:60718582-60718601 Msa0174940:intron 25.0%
!! AATCATGGAATCACAAATTT+TGG + chr1_4:60718597-60718616 Msa0174940:intron 25.0%
! ATAGTAAGGGTTTATATGAA+GGG - chr1_4:60718969-60718988 None:intergenic 25.0%
! AAGAATAGAATTTGCCAATT+TGG + chr1_4:60719465-60719484 Msa0174940:CDS 25.0%
!! TGGTTAGTCGATATTAATTT+AGG + chr1_4:60719560-60719579 Msa0174940:exon 25.0%
! TTGAAGTTTCACAAAAAAAC+CGG - chr1_4:60719638-60719657 None:intergenic 25.0%
TAACTGAAAAAAAACCTTCG+CGG - chr1_4:60717410-60717429 None:intergenic 30.0%
TTACTACTAACAAAAACACC+AGG + chr1_4:60717564-60717583 Msa0174940:CDS 30.0%
!! TTGATTTGTCTATATGCATG+TGG + chr1_4:60717685-60717704 Msa0174940:intron 30.0%
!! ATTGCAGCTGAATTTGATTT+TGG + chr1_4:60717708-60717727 Msa0174940:intron 30.0%
! AGATTTTGTTAGCATGTATG+AGG + chr1_4:60717952-60717971 Msa0174940:intron 30.0%
AGCACAATCAATATCAATCA+CGG - chr1_4:60717979-60717998 None:intergenic 30.0%
GTTTGTCTGTATGATTATTC+AGG + chr1_4:60718048-60718067 Msa0174940:intron 30.0%
!!! GGAGTTTTGAGATGAAAAAT+AGG + chr1_4:60718129-60718148 Msa0174940:CDS 30.0%
CTTGCTTATTTCCACTAATT+TGG + chr1_4:60718373-60718392 Msa0174940:intron 30.0%
ACAAAAGCAACAACACAAAT+AGG - chr1_4:60718574-60718593 None:intergenic 30.0%
!!! ACACAGTTAATTGTTTTGGT+GGG + chr1_4:60718852-60718871 Msa0174940:CDS 30.0%
ATCAAGGTTATTGGAAATAC+TGG + chr1_4:60718878-60718897 Msa0174940:CDS 30.0%
CATAGTAAGGGTTTATATGA+AGG - chr1_4:60718970-60718989 None:intergenic 30.0%
CTTCATATAAACCCTTACTA+TGG + chr1_4:60718968-60718987 Msa0174940:CDS 30.0%
!!! GCTAAGTTTTGAATTACATG+CGG - chr1_4:60719097-60719116 None:intergenic 30.0%
CTGATAATAAAATCGTTGTG+TGG + chr1_4:60719179-60719198 Msa0174940:CDS 30.0%
AAGAGCTTAGAATTCAATGT+AGG - chr1_4:60719325-60719344 None:intergenic 30.0%
AAGGATACAATAAGTCTACA+AGG - chr1_4:60719612-60719631 None:intergenic 30.0%
AATTATGTATGTTGTTGTGC+TGG + chr1_4:60719664-60719683 Msa0174940:three_prime_UTR 30.0%
! TAAGATCTTGTTTCTTGTAC+TGG + chr1_4:60719706-60719725 Msa0174940:three_prime_UTR 30.0%
ACAAGGTTAATACAATATGC+AGG - chr1_4:60719756-60719775 None:intergenic 30.0%
TGTTTGGCATATTTCAAACA+AGG - chr1_4:60719773-60719792 None:intergenic 30.0%
!! ACTTCTAAAATTGCATGAGT+GGG - chr1_4:60719814-60719833 None:intergenic 30.0%
! TTTTTGCTTAAAATGCTGAG+AGG + chr1_4:60719838-60719857 Msa0174940:three_prime_UTR 30.0%
!!! GCGAAGGTTTTTTTTCAGTT+AGG + chr1_4:60717409-60717428 Msa0174940:five_prime_UTR 35.0%
! ATTTGAAGTTCACTGAGGTA+TGG + chr1_4:60717475-60717494 Msa0174940:exon 35.0%
TTGTTAGTAGTAAGATCGGA+AGG - chr1_4:60717557-60717576 None:intergenic 35.0%
GAAAACGGAAAATTTGAACC+TGG - chr1_4:60717585-60717604 None:intergenic 35.0%
ACCATTCCTGTATCTTTGTA+TGG + chr1_4:60717899-60717918 Msa0174940:intron 35.0%
TCCATACAAAGATACAGGAA+TGG - chr1_4:60717903-60717922 None:intergenic 35.0%
TCTTGTCCATACAAAGATAC+AGG - chr1_4:60717908-60717927 None:intergenic 35.0%
GCACAATCAATATCAATCAC+GGG - chr1_4:60717978-60717997 None:intergenic 35.0%
GATTATTCAGGAACTCTTAC+TGG + chr1_4:60718060-60718079 Msa0174940:intron 35.0%
CAAGCATTATTCCACTAAGT+GGG + chr1_4:60718214-60718233 Msa0174940:intron 35.0%
AAGCATTATTCCACTAAGTG+GGG + chr1_4:60718215-60718234 Msa0174940:intron 35.0%
! TTCTGGAAGAGACACAAATA+TGG + chr1_4:60718295-60718314 Msa0174940:intron 35.0%
TATTTACAGGACTTGTACAG+AGG + chr1_4:60718640-60718659 Msa0174940:intron 35.0%
!! AGGTAGGTATGGAGTTTTAA+TGG - chr1_4:60718693-60718712 None:intergenic 35.0%
TGAAGGTCAATTAATGAGCT+GGG + chr1_4:60718805-60718824 Msa0174940:CDS 35.0%
!!! CACACAGTTAATTGTTTTGG+TGG + chr1_4:60718851-60718870 Msa0174940:CDS 35.0%
!!! TTGTTTTGGTGGGAAAATCA+AGG + chr1_4:60718862-60718881 Msa0174940:CDS 35.0%
! ATGAGGTTTTGCCATAGTAA+GGG - chr1_4:60718982-60719001 None:intergenic 35.0%
! AATGAGGTTTTGCCATAGTA+AGG - chr1_4:60718983-60719002 None:intergenic 35.0%
TTACTATGGCAAAACCTCAT+TGG + chr1_4:60718982-60719001 Msa0174940:CDS 35.0%
TTTCAAAGTGTGCTTTCCAT+GGG + chr1_4:60719022-60719041 Msa0174940:CDS 35.0%
GGCACATTATATATGATCGT+GGG - chr1_4:60719049-60719068 None:intergenic 35.0%
AGGCACATTATATATGATCG+TGG - chr1_4:60719050-60719069 None:intergenic 35.0%
!!! AAGTTTTGAATTACATGCGG+TGG - chr1_4:60719094-60719113 None:intergenic 35.0%
CTTAGCTCAAAATACTTGCT+TGG + chr1_4:60719111-60719130 Msa0174940:CDS 35.0%
AATAAAATCGTTGTGTGGAG+CGG + chr1_4:60719184-60719203 Msa0174940:CDS 35.0%
!! AAATCCCATTGGTTTGAACA+CGG + chr1_4:60719375-60719394 Msa0174940:CDS 35.0%
! ATTCTTTTCTCTAATGGGAG+AGG - chr1_4:60719451-60719470 None:intergenic 35.0%
! AGAATTTGCCAATTTGGTGA+AGG + chr1_4:60719471-60719490 Msa0174940:CDS 35.0%
ACCTATTTCCTTCACCAAAT+TGG - chr1_4:60719482-60719501 None:intergenic 35.0%
! ATAGGTCGTCAACATTTTGT+TGG + chr1_4:60719496-60719515 Msa0174940:CDS 35.0%
!! TGATGACAACGACTTTTTCT+TGG + chr1_4:60719540-60719559 Msa0174940:CDS 35.0%
GACTTATTGTATCCTTTCTC+CGG + chr1_4:60719616-60719635 Msa0174940:three_prime_UTR 35.0%
TTCACAAAAAAACCGGAGAA+AGG - chr1_4:60719631-60719650 None:intergenic 35.0%
!! CTTGTACTGGTTACTTTGTT+TGG + chr1_4:60719719-60719738 Msa0174940:three_prime_UTR 35.0%
!! CGTTTTTGAAAATGGGTGTT+TGG - chr1_4:60719789-60719808 None:intergenic 35.0%
!! GTGGGATCGTTTTTGAAAAT+GGG - chr1_4:60719796-60719815 None:intergenic 35.0%
!! AGTGGGATCGTTTTTGAAAA+TGG - chr1_4:60719797-60719816 None:intergenic 35.0%
!! CACTTCTAAAATTGCATGAG+TGG - chr1_4:60719815-60719834 None:intergenic 35.0%
GCTACATTGAAGAGCTATAC+TGG + chr1_4:60717497-60717516 Msa0174940:CDS 40.0%
TATTCAGGAACTCTTACTGG+AGG + chr1_4:60718063-60718082 Msa0174940:intron 40.0%
TCATCTCAAAACTCCGCATT+GGG - chr1_4:60718124-60718143 None:intergenic 40.0%
TTCATCTCAAAACTCCGCAT+TGG - chr1_4:60718125-60718144 None:intergenic 40.0%
CCACTTAGTGGAATAATGCT+TGG - chr1_4:60718216-60718235 None:intergenic 40.0%
CCAAGCATTATTCCACTAAG+TGG + chr1_4:60718213-60718232 Msa0174940:intron 40.0%
ATTATTCCACTAAGTGGGGT+TGG + chr1_4:60718219-60718238 Msa0174940:intron 40.0%
ATACTCCTTAGAACATGCAG+CGG - chr1_4:60718519-60718538 None:intergenic 40.0%
AAAACTCCATACCTACCTGA+TGG + chr1_4:60718695-60718714 Msa0174940:CDS 40.0%
TCAAGATCAAATCGACCATC+AGG - chr1_4:60718713-60718732 None:intergenic 40.0%
CTCGATCTGCATATTTACCA+AGG - chr1_4:60718747-60718766 None:intergenic 40.0%
GTAAATATGCAGATCGAGAG+TGG + chr1_4:60718749-60718768 Msa0174940:CDS 40.0%
!! GGAGTTGAAGGTGTTATTGT+TGG + chr1_4:60718770-60718789 Msa0174940:CDS 40.0%
!! GTTGAAGGTGTTATTGTTGG+TGG + chr1_4:60718773-60718792 Msa0174940:CDS 40.0%
GTGAAGGTCAATTAATGAGC+TGG + chr1_4:60718804-60718823 Msa0174940:CDS 40.0%
! GCGCACACAGTTAATTGTTT+TGG + chr1_4:60718848-60718867 Msa0174940:CDS 40.0%
GGTGGGAAAATCAAGGTTAT+TGG + chr1_4:60718869-60718888 Msa0174940:CDS 40.0%
ATACTGGAAGTAACTCGACA+AGG + chr1_4:60718894-60718913 Msa0174940:CDS 40.0%
TACTGGAAGTAACTCGACAA+GGG + chr1_4:60718895-60718914 Msa0174940:CDS 40.0%
TATGGCAAAACCTCATTGGA+TGG + chr1_4:60718986-60719005 Msa0174940:CDS 40.0%
CAAAACCTCATTGGATGGTA+TGG + chr1_4:60718991-60719010 Msa0174940:CDS 40.0%
CTTTCAAAGTGTGCTTTCCA+TGG + chr1_4:60719021-60719040 Msa0174940:CDS 40.0%
TATAATGTGCCTTCACGAAC+TGG + chr1_4:60719058-60719077 Msa0174940:CDS 40.0%
ATAAAATCGTTGTGTGGAGC+GGG + chr1_4:60719185-60719204 Msa0174940:CDS 40.0%
ATCAATGCCATGATGCTAGA+TGG + chr1_4:60719215-60719234 Msa0174940:CDS 40.0%
TCAATGCCATGATGCTAGAT+GGG + chr1_4:60719216-60719235 Msa0174940:CDS 40.0%
ATGAGTTCTCGCATTAAACC+AGG - chr1_4:60719286-60719305 None:intergenic 40.0%
AATGCGAGAACTCATGTTTG+AGG + chr1_4:60719291-60719310 Msa0174940:CDS 40.0%
ATGCGAGAACTCATGTTTGA+GGG + chr1_4:60719292-60719311 Msa0174940:CDS 40.0%
TGGAAAAGCCAGTCAATCAA+AGG - chr1_4:60719349-60719368 None:intergenic 40.0%
GTGTTCAAACCAATGGGATT+TGG - chr1_4:60719376-60719395 None:intergenic 40.0%
ACAAATGGCAACCTGAAAAC+TGG - chr1_4:60719427-60719446 None:intergenic 40.0%
! TCTAATGGGAGAGGTACAAA+TGG - chr1_4:60719442-60719461 None:intergenic 40.0%
! GCCAATTTGGTGAAGGAAAT+AGG + chr1_4:60719478-60719497 Msa0174940:CDS 40.0%
TGTTGGAACTGAAGATGTTC+AGG + chr1_4:60719513-60719532 Msa0174940:CDS 40.0%
AGAGGTAGACATCAAACCAA+TGG + chr1_4:60719856-60719875 Msa0174940:three_prime_UTR 40.0%
AACTTCAAATCGAGCTGCTG+TGG - chr1_4:60717465-60717484 None:intergenic 45.0%
! GCTCGATTTGAAGTTCACTG+AGG + chr1_4:60717470-60717489 Msa0174940:five_prime_UTR 45.0%
TATACTGGTGTCTGCTTCAG+AGG + chr1_4:60717512-60717531 Msa0174940:CDS 45.0%
GTAAGATCGGAAGGATGAAC+CGG - chr1_4:60717548-60717567 None:intergenic 45.0%
GATTTCACAGCTGCTTGTGT+AGG - chr1_4:60718089-60718108 None:intergenic 45.0%
AATCGACCATCAGGTAGGTA+TGG - chr1_4:60718704-60718723 None:intergenic 45.0%
GATCAAATCGACCATCAGGT+AGG - chr1_4:60718709-60718728 None:intergenic 45.0%
TCTTGAAGCATACGATGCCT+TGG + chr1_4:60718727-60718746 Msa0174940:CDS 45.0%
!! GTTATTGTTGGTGGCACAAC+TGG + chr1_4:60718782-60718801 Msa0174940:CDS 45.0%
AGCAACCATACCATCCAATG+AGG - chr1_4:60718999-60719018 None:intergenic 45.0%
ATATATGATCGTGGGTCCCA+TGG - chr1_4:60719041-60719060 None:intergenic 45.0%
ATGTCTTGACCAGTTCGTGA+AGG - chr1_4:60719070-60719089 None:intergenic 45.0%
GCTCAAAATACTTGCTTGGC+TGG + chr1_4:60719115-60719134 Msa0174940:CDS 45.0%
AGGAATGTGTGGGAAATGAC+AGG + chr1_4:60719143-60719162 Msa0174940:CDS 45.0%
CAATGCCATGATGCTAGATG+GGG + chr1_4:60719217-60719236 Msa0174940:CDS 45.0%
CATAACCCCATCTAGCATCA+TGG - chr1_4:60719225-60719244 None:intergenic 45.0%
CATGATGCTAGATGGGGTTA+TGG + chr1_4:60719223-60719242 Msa0174940:CDS 45.0%
GTTGCAAGCAATCTGATTCC+TGG + chr1_4:60719265-60719284 Msa0174940:CDS 45.0%
AGCTCTTGCCTTTGATTGAC+TGG + chr1_4:60719338-60719357 Msa0174940:CDS 45.0%
TTCCACATGCCAAATCCCAT+TGG + chr1_4:60719364-60719383 Msa0174940:CDS 45.0%
AACCAATGGGATTTGGCATG+TGG - chr1_4:60719369-60719388 None:intergenic 45.0%
AGAGCCGTGTTCAAACCAAT+GGG - chr1_4:60719382-60719401 None:intergenic 45.0%
AAGAGCCGTGTTCAAACCAA+TGG - chr1_4:60719383-60719402 None:intergenic 45.0%
TTGCTCAACTTGGAGTTGTC+AGG + chr1_4:60719401-60719420 Msa0174940:CDS 45.0%
GGTTTCTCAGTAAACCGCGA+AGG + chr1_4:60717393-60717412 Msa0174940:five_prime_UTR 50.0%
! CTTCAGAGGATCCGGTTTTC+CGG + chr1_4:60717526-60717545 Msa0174940:CDS 50.0%
GGAAGGATGAACCGGAAAAC+CGG - chr1_4:60717540-60717559 None:intergenic 50.0%
CCAATTTCCACCTCCCAATG+CGG + chr1_4:60718108-60718127 Msa0174940:CDS 50.0%
TCTCAAAACTCCGCATTGGG+AGG - chr1_4:60718121-60718140 None:intergenic 50.0%
CTAATGCCAACCCCACTTAG+TGG - chr1_4:60718228-60718247 None:intergenic 50.0%
CTGTACCGCTGCATGTTCTA+AGG + chr1_4:60718511-60718530 Msa0174940:intron 50.0%
CAGATCGAGAGTGGAGTTGA+AGG + chr1_4:60718758-60718777 Msa0174940:CDS 50.0%
GCAATTCATGCCACTGAGCA+AGG + chr1_4:60718920-60718939 Msa0174940:CDS 50.0%
! TGCCACTGAGCAAGGTTTTG+CGG + chr1_4:60718928-60718947 Msa0174940:CDS 50.0%
AACCGCAAAACCTTGCTCAG+TGG - chr1_4:60718933-60718952 None:intergenic 50.0%
! ACTGAGCAAGGTTTTGCGGT+TGG + chr1_4:60718932-60718951 Msa0174940:CDS 50.0%
! GGTTTTGCGGTTGGAATGCA+TGG + chr1_4:60718941-60718960 Msa0174940:CDS 50.0%
! TACTTGCTTGGCTGGTGTCA+AGG + chr1_4:60719123-60719142 Msa0174940:CDS 50.0%
! GCTGGTGTCAAGGAATGTGT+GGG + chr1_4:60719133-60719152 Msa0174940:CDS 50.0%
AGATGGGGTTATGGAGCTAC+TGG + chr1_4:60719232-60719251 Msa0174940:CDS 50.0%
AACACGGCTCTTGCTCAACT+TGG + chr1_4:60719391-60719410 Msa0174940:CDS 50.0%
! GAGTTGTCAGGCCAGTTTTC+AGG + chr1_4:60719413-60719432 Msa0174940:CDS 50.0%
TCGAGCTGCTGTGGAGAAGA+CGG - chr1_4:60717456-60717475 None:intergenic 55.0%
GGTGTCTGCTTCAGAGGATC+CGG + chr1_4:60717518-60717537 Msa0174940:CDS 55.0%
CCGCATTGGGAGGTGGAAAT+TGG - chr1_4:60718111-60718130 None:intergenic 55.0%
CAAAACTCCGCATTGGGAGG+TGG - chr1_4:60718118-60718137 None:intergenic 55.0%
AAGGTGGTCTCGCTTGCTTC+TGG + chr1_4:60718545-60718564 Msa0174940:intron 55.0%
!! GTTGGTGGCACAACTGGTGA+AGG + chr1_4:60718788-60718807 Msa0174940:CDS 55.0%
! GGCTGGTGTCAAGGAATGTG+TGG + chr1_4:60719132-60719151 Msa0174940:CDS 55.0%
GTGGAGAAGACGGTGGTTGG+CGG - chr1_4:60717446-60717465 None:intergenic 60.0%
GCTGTGGAGAAGACGGTGGT+TGG - chr1_4:60717449-60717468 None:intergenic 60.0%
AGCTGCTGTGGAGAAGACGG+TGG - chr1_4:60717453-60717472 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 60717362 60719899 60717362 ID=Msa0174940;Name=Msa0174940
chr1_4 mRNA 60717362 60719899 60717362 ID=Msa0174940-mRNA-1;Parent=Msa0174940;Name=Msa0174940-mRNA-1;_AED=0.02;_eAED=0.02;_QI=132|1|1|1|1|1|3|323|366
chr1_4 exon 60717362 60717585 60717362 ID=Msa0174940-mRNA-1:exon:19257;Parent=Msa0174940-mRNA-1
chr1_4 exon 60718070 60718150 60718070 ID=Msa0174940-mRNA-1:exon:19258;Parent=Msa0174940-mRNA-1
chr1_4 exon 60718649 60719899 60718649 ID=Msa0174940-mRNA-1:exon:19259;Parent=Msa0174940-mRNA-1
chr1_4 five_prime_UTR 60717362 60717493 60717362 ID=Msa0174940-mRNA-1:five_prime_utr;Parent=Msa0174940-mRNA-1
chr1_4 CDS 60717494 60717585 60717494 ID=Msa0174940-mRNA-1:cds;Parent=Msa0174940-mRNA-1
chr1_4 CDS 60718070 60718150 60718070 ID=Msa0174940-mRNA-1:cds;Parent=Msa0174940-mRNA-1
chr1_4 CDS 60718649 60719576 60718649 ID=Msa0174940-mRNA-1:cds;Parent=Msa0174940-mRNA-1
chr1_4 three_prime_UTR 60719577 60719899 60719577 ID=Msa0174940-mRNA-1:three_prime_utr;Parent=Msa0174940-mRNA-1
Gene Sequence

>Msa0174940

ATGGCTACATTGAAGAGCTATACTGGTGTCTGCTTCAGAGGATCCGGTTTTCCGGTTCATCCTTCCGATCTTACTACTAACAAAAACACCAGGAACTCTTACTGGAGGCCTACACAAGCAGCTGTGAAATCCAATTTCCACCTCCCAATGCGGAGTTTTGAGATGAAAAATAGGACTTGTACAGAGGACATTAAGTGTCTGAGATTGATAACTGCCATTAAAACTCCATACCTACCTGATGGTCGATTTGATCTTGAAGCATACGATGCCTTGGTAAATATGCAGATCGAGAGTGGAGTTGAAGGTGTTATTGTTGGTGGCACAACTGGTGAAGGTCAATTAATGAGCTGGGAAGAGCACATAATGCTTATTGCGCACACAGTTAATTGTTTTGGTGGGAAAATCAAGGTTATTGGAAATACTGGAAGTAACTCGACAAGGGAAGCAATTCATGCCACTGAGCAAGGTTTTGCGGTTGGAATGCATGGTGCCCTTCATATAAACCCTTACTATGGCAAAACCTCATTGGATGGTATGGTTGCTCACTTTCAAAGTGTGCTTTCCATGGGACCCACGATCATATATAATGTGCCTTCACGAACTGGTCAAGACATTCCACCGCATGTAATTCAAAACTTAGCTCAAAATACTTGCTTGGCTGGTGTCAAGGAATGTGTGGGAAATGACAGGATCAAAGAGTATACTGATAATAAAATCGTTGTGTGGAGCGGGAACGATGATCAATGCCATGATGCTAGATGGGGTTATGGAGCTACTGGAGTGATATCTGTTGCAAGCAATCTGATTCCTGGTTTAATGCGAGAACTCATGTTTGAGGGCAAAAATCCTACATTGAATTCTAAGCTCTTGCCTTTGATTGACTGGCTTTTCCACATGCCAAATCCCATTGGTTTGAACACGGCTCTTGCTCAACTTGGAGTTGTCAGGCCAGTTTTCAGGTTGCCATTTGTACCTCTCCCATTAGAGAAAAGAATAGAATTTGCCAATTTGGTGAAGGAAATAGGTCGTCAACATTTTGTTGGAACTGAAGATGTTCAGGTTCTTGATGACAACGACTTTTTCTTGGTTAGTCGATATTAA
Protein sequence

>Msa0174940

MATLKSYTGVCFRGSGFPVHPSDLTTNKNTRNSYWRPTQAAVKSNFHLPMRSFEMKNRTCTEDIKCLRLITAIKTPYLPDGRFDLEAYDALVNMQIESGVEGVIVGGTTGEGQLMSWEEHIMLIAHTVNCFGGKIKVIGNTGSNSTREAIHATEQGFAVGMHGALHINPYYGKTSLDGMVAHFQSVLSMGPTIIYNVPSRTGQDIPPHVIQNLAQNTCLAGVKECVGNDRIKEYTDNKIVVWSGNDDQCHDARWGYGATGVISVASNLIPGLMRELMFEGKNPTLNSKLLPLIDWLFHMPNPIGLNTALAQLGVVRPVFRLPFVPLPLEKRIEFANLVKEIGRQHFVGTEDVQVLDDNDFFLVSRY*