AlfalfaGEDB | Msa0186620

M. sativa cultivar zhongmu-4 / Msa0186620

KEGG: K10273
GO: GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0032446 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0070647 GO:0071704 GO:0140096 GO:1901564
PFAM: PF00646.36

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0186620	XP_003589128.1	98.720	547	7	0	1	547	1	547	0.0	1095

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0186620	sp\|Q9C626\|FB37_ARATH	66.116	484	158	4	55	536	34	513	0.0	626

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0186620	Q2HTR2	98.720	547	7	0	1	547	1	547	0.0	1095

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0052440	Msa0186620	0.870610	1.281610e-66	-8.615850e-47
Msa0097070	Msa0186620	0.999186	8.393098e-295	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0186620	MtrunA17_Chr1g0152951	98.720	547	7	0	1	547	1	547	0.0	1095
Msa0186620	MtrunA17_Chr4g0053361	54.472	492	218	3	52	538	34	524	0.0	538
Msa0186620	MtrunA17_Chr2g0286991	28.541	466	251	16	59	449	5	463	1.76e-29	122
Msa0186620	MtrunA17_Chr2g0286991	26.171	363	224	12	98	453	245	570	1.49e-17	85.9
Msa0186620	MtrunA17_Chr4g0048391	23.061	490	246	18	57	443	19	480	2.12e-17	85.5
Msa0186620	MtrunA17_Chr7g0251831	24.390	492	259	17	61	446	1	485	5.88e-16	80.9
Msa0186620	MtrunA17_Chr1g0196061	21.756	501	256	17	64	446	14	496	1.66e-15	79.7
Msa0186620	MtrunA17_Chr1g0212071	24.471	425	230	13	66	455	23	391	4.63e-15	77.4
Msa0186620	MtrunA17_Chr4g0004901	26.923	260	181	6	187	443	365	618	5.54e-15	78.2
Msa0186620	MtrunA17_Chr4g0004901	29.600	250	168	6	134	381	389	632	6.16e-15	78.2

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0186620	AT1G47056.1	66.116	484	158	4	55	536	34	513	0.0	626
Msa0186620	AT4G07400.1	61.154	520	185	6	14	531	35	539	0.0	605
Msa0186620	AT5G67250.1	55.985	543	209	6	1	538	1	518	0.0	552
Msa0186620	AT3G50080.1	56.790	486	200	6	56	538	36	514	0.0	530
Msa0186620	AT4G15475.1	29.394	330	211	13	127	447	274	590	6.21e-23	103
Msa0186620	AT4G15475.1	27.696	408	243	12	51	453	3	363	3.65e-22	100
Msa0186620	AT2G25490.1	26.107	429	273	16	61	453	64	484	4.91e-17	85.1
Msa0186620	AT2G25490.1	25.000	360	203	9	134	432	212	565	3.66e-14	75.9
Msa0186620	AT5G27920.1	27.019	359	213	13	137	459	158	503	7.71e-16	81.3
Msa0186620	AT5G27920.1	26.087	299	148	9	158	446	332	567	1.61e-11	67.4

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Find 150 sgRNAs with CRISPR-Local

Find 312 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGCCAATCTCTCTAGATT+CGG	0.392075	2_1:+1348070	None:intergenic
AAAGGTTAGGGCTACTGTCC+GGG	0.502510	2_1:-1347667	Msa0186620:CDS
AACATCGTCACCGTCGTCTG+TGG	0.629183	2_1:+1349116	None:intergenic
AACCAACAGATCCACCGCCT+TGG	0.526292	2_1:-1349154	Msa0186620:CDS
AACCAAGGCGGTGGATCTGT+TGG	0.533705	2_1:+1349152	None:intergenic
AACGACGCAGCGGCCACACC+CGG	0.561295	2_1:+1348547	None:intergenic
AAGCCAATCTCTCTAGATTC+GGG	0.481744	2_1:+1348071	None:intergenic
AAGGGTGTTACACCTGAAGG+CGG	0.687864	2_1:-1347884	Msa0186620:CDS
AATCGTCTTCAACGACGCAG+CGG	0.706961	2_1:+1348537	None:intergenic
AATTGTCGGTGAAGCGGCTT+AGG	0.348422	2_1:-1348606	Msa0186620:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTAAACAGAGAAGGAATTAC+CGG	+	chr2_1:1347937-1347956	None:intergenic	30.0%
TCAAATCGATTAAACAGAGA+AGG	+	chr2_1:1347946-1347965	None:intergenic	30.0%
CTGAAAGATGTAAGTTGTTA+AGG	-	chr2_1:1348583-1348602	Msa0186620:CDS	30.0%
TTAAGGAAGCTACATATTGA+TGG	-	chr2_1:1348600-1348619	Msa0186620:CDS	30.0%
TTAAACAGAGAAGGAATTAC+CGG	+	chr2_1:1347937-1347956	None:intergenic	30.0%
TCAAATCGATTAAACAGAGA+AGG	+	chr2_1:1347946-1347965	None:intergenic	30.0%
CTGAAAGATGTAAGTTGTTA+AGG	-	chr2_1:1348583-1348602	Msa0186620:CDS	30.0%
TTAAGGAAGCTACATATTGA+TGG	-	chr2_1:1348600-1348619	Msa0186620:CDS	30.0%
ACAATCGCTAAACATTCATC+AGG	+	chr2_1:1347796-1347815	None:intergenic	35.0%
CTAAGAATTGTAAGGGTTTG+AGG	-	chr2_1:1348118-1348137	Msa0186620:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_1	gene	1347601	1349244	1347601	ID=Msa0186620;Name=Msa0186620
chr2_1	mRNA	1347601	1349244	1347601	ID=Msa0186620-mRNA-1;Parent=Msa0186620;Name=Msa0186620-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|547
chr2_1	exon	1347601	1349244	1347601	ID=Msa0186620-mRNA-1:exon:572;Parent=Msa0186620-mRNA-1
chr2_1	CDS	1347601	1349244	1347601	ID=Msa0186620-mRNA-1:cds;Parent=Msa0186620-mRNA-1

Gene Sequence

>Msa0186620

ATGGGCCAAGCAGCTTCCACGGTCACCGGAACCAACCGCCGGGAAAGTGCAGGCAACCGATCTACCAAAACCAACAGATCCACCGCCTTGGTTTCTCCGATGATCTCCACAGACGACGGTGACGATGTTGCAGATCCGGTTGACGGTGACGGTAACGACGGAATCGGAAACTCCGATTATATATCAGAACTACCTGATGAATGTTTAGCGATTGTGTTTCAGTCGTTGAATCCCTCTGATCGGAATCAGTGTTCACTCGTTTGCCGGCGGTGGCTTCACGTTGAAGGACAGAGTCGTCAACGGCTTTCGTTGAACGCGAAGTTTGATCTTCTTCCGGTAATTCCTTCTCTGTTTAATCGATTTGATTCAGTGACGAAATTAGCGTTGAAGTGTGATCGTAGATCTGCGAGTATTAGAGATGAAGCGCTTGTGATAATCTCTGAGCGTTGTCCTAATCTCACGCGCCTTAAGCTCCGCGCGTGTCGCGAACTTACGGATGCTGGAATGGAAGCTTTTGCTAAGAATTGTAAGGGTTTGAGGAAATTATCGTGTGGATCTTGTACTTTTGGATCTAAAGGTATGAACGCGGTGCTTGAAAATTGCGCCGCGCTCGAGGAATTGTCGGTGAAGCGGCTTAGGGGAATTGCTGAGACTGCGGTGGCTGAGCCGATTGGACCGGGTGTGGCCGCTGCGTCGTTGAAGACGATTTGTTTGAAGGAACTTTATAATGGACAGTGTTTTGGTTCGTTGATTTTAGGTGCTAAGAATTTGAAGACTTTGAAGCTATTTCGATGTTCCGGTGATTGGGATACGCTTTTTACACTTATGGCTGAAAGAGTGTCGAGTATGATTGTTGAGGTTCATTTTGAGAGGCTTCAGATTAGTGATATTGGATTGCAAGCTATTTCGAATTGTTCGAATTTGGAGATTTTGCATCTTGTTAAGACACCTGAGTGTACTGATATGGGACTTGTTGCGATTGCTGAAAGATGTAAGTTGTTAAGGAAGCTACATATTGATGGATGGAAGGCAAATCGGATTGGCGATGAAGGGTTAATTGCGGTTGCAAAGTTTTGCCCTAATTTGCAAGAATTGGTGCTTATTGGTGTTAACCCAACGAGAGTGAGTTTGGAAATGTTGGCATCGAATTGCCCGAATCTAGAGAGATTGGCTTTGTGTGCAAGTGATACTGTTGGTGATCCTGAGATATCTTGCATTGCTGCAAAGTGTTTGGCTTTGAAGAAACTTTGCATCAAGAGTTGTCCTGTTTCTGATCTTGGGATGGAAGCTTTGGCACATGGGTGTCCCAATTTGGTGAAAGTGAAGGTCAAGAAGTGTAAGGGTGTTACACCTGAAGGCGGGGATTGGTTGAGGCACACTAGGGTTTCCGTAGCTGTGAATCTGGACGCGGTTGAAGCAGAACTTCAAGATGCTAGTGCTAGTGATGGTGGAGCACAAGAGAATGGGATTGAATTTCCTTCAATGCCCGGCTCCGCGGCATCTACATCGGCAAATATTGCATCGAGAAGTACTGTTCGATCGAGTTCATTCAAGCAAAGGTTAGGGCTACTGTCCGGGAGGAAAATTGTGGCTAGCACTTTGAGAAGATGGTCAGGTGGTAGCACTAGTGCCCGTCATGGTTAG

Protein sequence

>Msa0186620

MGQAASTVTGTNRRESAGNRSTKTNRSTALVSPMISTDDGDDVADPVDGDGNDGIGNSDYISELPDECLAIVFQSLNPSDRNQCSLVCRRWLHVEGQSRQRLSLNAKFDLLPVIPSLFNRFDSVTKLALKCDRRSASIRDEALVIISERCPNLTRLKLRACRELTDAGMEAFAKNCKGLRKLSCGSCTFGSKGMNAVLENCAALEELSVKRLRGIAETAVAEPIGPGVAAASLKTICLKELYNGQCFGSLILGAKNLKTLKLFRCSGDWDTLFTLMAERVSSMIVEVHFERLQISDIGLQAISNCSNLEILHLVKTPECTDMGLVAIAERCKLLRKLHIDGWKANRIGDEGLIAVAKFCPNLQELVLIGVNPTRVSLEMLASNCPNLERLALCASDTVGDPEISCIAAKCLALKKLCIKSCPVSDLGMEALAHGCPNLVKVKVKKCKGVTPEGGDWLRHTRVSVAVNLDAVEAELQDASASDGGAQENGIEFPSMPGSAASTSANIASRSTVRSSSFKQRLGLLSGRKIVASTLRRWSGGSTSARHG*