AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0186910

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0186910 MtrunA17_Chr8g0381291 97.902 143 3 0 4 146 1 143 4.02e-99 281
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0186910 AT1G16790.1 54.464 112 48 2 28 137 24 134 6.02e-41 134

Find 36 sgRNAs with CRISPR-Local

Find 130 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGCAATTGGGGTTGTTTC+TGG 0.158557 2_1:+1659642 Msa0186910:CDS
GTTTCTGGTGCTGCTCTTAA+TGG 0.209111 2_1:+1659657 Msa0186910:CDS
CATTCCTTGTGATTCATAAT+AGG 0.265305 2_1:-1659433 Msa0186920:three_prime_UTR
TTCATCTGGTAAAGGTAAAA+AGG 0.279247 2_1:+1659518 Msa0186910:CDS
TTTCACCTATTGCTCAGATT+TGG 0.309135 2_1:+1659706 Msa0186910:CDS
GATTTACAGAAGGCGTTGTT+TGG 0.358146 2_1:+1659489 Msa0186910:CDS
GGTGCTGCTCTTAATGGAAT+TGG 0.362930 2_1:+1659663 Msa0186910:CDS
TGTTTAATCAGTGGTTGAAA+TGG 0.375434 2_1:+1659317 Msa0186910:intron
TTGTTGAGACGTTTGCAATT+GGG 0.377010 2_1:+1659631 Msa0186910:CDS
TAATAGGATATCAATTCCTT+TGG 0.387539 2_1:-1659417 Msa0186920:three_prime_UTR
TCAGGTATAGAAGGTACATT+TGG 0.388079 2_1:-1659354 Msa0186920:three_prime_UTR
TTTGTTGAGACGTTTGCAAT+TGG 0.400517 2_1:+1659630 Msa0186910:CDS
GAATTCCAAATCTGAGCAAT+AGG 0.408372 2_1:-1659711 Msa0186920:three_prime_UTR
TTGTTTGGAGTAATTTCATC+TGG 0.412151 2_1:+1659504 Msa0186910:CDS
AAATGAATCTTCTAGAAAGA+TGG 0.419369 2_1:+1659551 Msa0186910:CDS
TCATTGAGGGGTAGATTTAG+TGG 0.436309 2_1:-1659745 Msa0186920:three_prime_UTR
ATGAAGCTTGATACTAAGCC+TGG 0.457946 2_1:+1659606 Msa0186910:CDS
GGAGTAATTTCATCTGGTAA+AGG 0.473380 2_1:+1659510 Msa0186910:CDS
TATAGAAGGTACATTTGGCT+TGG 0.481909 2_1:-1659349 Msa0186920:three_prime_UTR
AGGGTACAAAATGTCATTGA+GGG 0.507238 2_1:-1659758 Msa0186920:three_prime_UTR
TGTTGAGACGTTTGCAATTG+GGG 0.507433 2_1:+1659632 Msa0186910:CDS
ATCTTCTAGAAAGATGGATT+CGG 0.522036 2_1:+1659557 Msa0186910:CDS
CTATTATGAATCACAAGGAA+TGG 0.524935 2_1:+1659434 Msa0186910:CDS
AGGGGTAGATTTAGTGGCTG+AGG 0.534389 2_1:-1659739 Msa0186920:three_prime_UTR
TTCACAAGAAGCCTCATTGA+AGG 0.549868 2_1:+1659458 Msa0186910:CDS
AAGGGTACAAAATGTCATTG+AGG 0.555917 2_1:-1659759 Msa0186920:three_prime_UTR
GCAAACGTCTCAACAAATCC+AGG 0.561055 2_1:-1659624 Msa0186920:three_prime_UTR
GATATCAATTCCTTTGGCGA+AGG 0.568099 2_1:-1659411 Msa0186920:three_prime_UTR
TCATTATAAAAGCAGAGCAT+AGG 0.578916 2_1:+1658690 None:intergenic
AAAGAAGATTCCTTCGCCAA+AGG 0.596578 2_1:+1659401 Msa0186910:CDS
AGCCTCATTGAAGGTCATAG+AGG 0.621483 2_1:+1659467 Msa0186910:CDS
GGTCATAGAGGATTTACAGA+AGG 0.636264 2_1:+1659479 Msa0186910:CDS
GGGTACAAAATGTCATTGAG+GGG 0.657891 2_1:-1659757 Msa0186920:three_prime_UTR
ATATCCTATTATGAATCACA+AGG 0.666482 2_1:+1659429 Msa0186910:CDS
ATCCTCTATGACCTTCAATG+AGG 0.666837 2_1:-1659469 Msa0186920:three_prime_UTR
GCAATAGGTGAAAGAATGTG+AGG 0.689368 2_1:-1659696 Msa0186920:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTGATTAAACAAATAAATAT+TGG - chr2_1:1659308-1659327 Msa0186920:three_prime_UTR 15.0%
!! ATATTTATTTGTTTAATCAG+TGG + chr2_1:1659308-1659327 Msa0186910:intron 15.0%
!! CTGATTAAACAAATAAATAT+TGG - chr2_1:1659308-1659327 Msa0186920:three_prime_UTR 15.0%
!! ATATTTATTTGTTTAATCAG+TGG + chr2_1:1659308-1659327 Msa0186910:intron 15.0%
!! AATAAAAACAACATTAGACT+GGG - chr2_1:1658804-1658823 Msa0186920:intron 20.0%
!!! TTTTGGTTTAATTTATGCTT+CGG - chr2_1:1659135-1659154 Msa0186920:intron 20.0%
!! TAAAAACTAATTCAAAGTAG+AGG - chr2_1:1659244-1659263 Msa0186920:intron 20.0%
!!! TTTTGTTTCATTTTGATTTC+AGG - chr2_1:1659375-1659394 Msa0186920:three_prime_UTR 20.0%
!! AATAAAAACAACATTAGACT+GGG - chr2_1:1658804-1658823 Msa0186920:intron 20.0%
!!! TTTTGGTTTAATTTATGCTT+CGG - chr2_1:1659135-1659154 Msa0186920:intron 20.0%
!! TAAAAACTAATTCAAAGTAG+AGG - chr2_1:1659244-1659263 Msa0186920:intron 20.0%
!!! TTTTGTTTCATTTTGATTTC+AGG - chr2_1:1659375-1659394 Msa0186920:three_prime_UTR 20.0%
! GAATAAAAACAACATTAGAC+TGG - chr2_1:1658805-1658824 Msa0186920:intron 25.0%
!!! TAATAATCATCGCTTTTGAT+AGG + chr2_1:1658853-1658872 Msa0186910:intron 25.0%
!! TTTGGTTTAATTTATGCTTC+GGG - chr2_1:1659134-1659153 Msa0186920:intron 25.0%
!!! ATTTTGATTTCAGGTATAGA+AGG - chr2_1:1659366-1659385 Msa0186920:three_prime_UTR 25.0%
! TAATAGGATATCAATTCCTT+TGG - chr2_1:1659420-1659439 Msa0186920:three_prime_UTR 25.0%
! ATATCCTATTATGAATCACA+AGG + chr2_1:1659429-1659448 Msa0186910:CDS 25.0%
!! AAATGAATCTTCTAGAAAGA+TGG + chr2_1:1659551-1659570 Msa0186910:CDS 25.0%
! GAATAAAAACAACATTAGAC+TGG - chr2_1:1658805-1658824 Msa0186920:intron 25.0%
!!! TAATAATCATCGCTTTTGAT+AGG + chr2_1:1658853-1658872 Msa0186910:intron 25.0%
!! TTTGGTTTAATTTATGCTTC+GGG - chr2_1:1659134-1659153 Msa0186920:intron 25.0%
!!! ATTTTGATTTCAGGTATAGA+AGG - chr2_1:1659366-1659385 Msa0186920:three_prime_UTR 25.0%
! TAATAGGATATCAATTCCTT+TGG - chr2_1:1659420-1659439 Msa0186920:three_prime_UTR 25.0%
! ATATCCTATTATGAATCACA+AGG + chr2_1:1659429-1659448 Msa0186910:CDS 25.0%
!! AAATGAATCTTCTAGAAAGA+TGG + chr2_1:1659551-1659570 Msa0186910:CDS 25.0%
AGCGTATATCAATATCAACA+CGG + chr2_1:1658923-1658942 Msa0186910:intron 30.0%
!!! AACCCTCCTTTATTTTGAAA+TGG + chr2_1:1659094-1659113 Msa0186910:intron 30.0%
!!! ACCCTCCTTTATTTTGAAAT+GGG + chr2_1:1659095-1659114 Msa0186910:intron 30.0%
TCCCATTTCAAAATAAAGGA+GGG - chr2_1:1659099-1659118 Msa0186920:intron 30.0%
AACCTCCCATTTCAAAATAA+AGG - chr2_1:1659103-1659122 Msa0186920:intron 30.0%
TGTTTAATCAGTGGTTGAAA+TGG + chr2_1:1659317-1659336 Msa0186910:intron 30.0%
CATTCCTTGTGATTCATAAT+AGG - chr2_1:1659436-1659455 Msa0186920:three_prime_UTR 30.0%
CTATTATGAATCACAAGGAA+TGG + chr2_1:1659434-1659453 Msa0186910:CDS 30.0%
! TTGTTTGGAGTAATTTCATC+TGG + chr2_1:1659504-1659523 Msa0186910:CDS 30.0%
TTCATCTGGTAAAGGTAAAA+AGG + chr2_1:1659518-1659537 Msa0186910:CDS 30.0%
! ATCTTCTAGAAAGATGGATT+CGG + chr2_1:1659557-1659576 Msa0186910:CDS 30.0%
AGCGTATATCAATATCAACA+CGG + chr2_1:1658923-1658942 Msa0186910:intron 30.0%
!!! AACCCTCCTTTATTTTGAAA+TGG + chr2_1:1659094-1659113 Msa0186910:intron 30.0%
!!! ACCCTCCTTTATTTTGAAAT+GGG + chr2_1:1659095-1659114 Msa0186910:intron 30.0%
TCCCATTTCAAAATAAAGGA+GGG - chr2_1:1659099-1659118 Msa0186920:intron 30.0%
AACCTCCCATTTCAAAATAA+AGG - chr2_1:1659103-1659122 Msa0186920:intron 30.0%
TGTTTAATCAGTGGTTGAAA+TGG + chr2_1:1659317-1659336 Msa0186910:intron 30.0%
CATTCCTTGTGATTCATAAT+AGG - chr2_1:1659436-1659455 Msa0186920:three_prime_UTR 30.0%
CTATTATGAATCACAAGGAA+TGG + chr2_1:1659434-1659453 Msa0186910:CDS 30.0%
! TTGTTTGGAGTAATTTCATC+TGG + chr2_1:1659504-1659523 Msa0186910:CDS 30.0%
TTCATCTGGTAAAGGTAAAA+AGG + chr2_1:1659518-1659537 Msa0186910:CDS 30.0%
! ATCTTCTAGAAAGATGGATT+CGG + chr2_1:1659557-1659576 Msa0186910:CDS 30.0%
GACAATAAAACAAACTGCGA+TGG - chr2_1:1659006-1659025 Msa0186920:intron 35.0%
! GAATTGTTGTTGCTTCACAA+TGG + chr2_1:1659069-1659088 Msa0186910:intron 35.0%
CTCCCATTTCAAAATAAAGG+AGG - chr2_1:1659100-1659119 Msa0186920:intron 35.0%
!!! CTCCTTTATTTTGAAATGGG+AGG + chr2_1:1659098-1659117 Msa0186910:intron 35.0%
CCAAAAAAACCAGTCTCTAA+CGG + chr2_1:1659149-1659168 Msa0186910:intron 35.0%
ATTGAAATGTACCAGTAGAC+AGG - chr2_1:1659208-1659227 Msa0186920:intron 35.0%
! TATAGAAGGTACATTTGGCT+TGG - chr2_1:1659352-1659371 Msa0186920:three_prime_UTR 35.0%
! TCAGGTATAGAAGGTACATT+TGG - chr2_1:1659357-1659376 Msa0186920:three_prime_UTR 35.0%
GGAGTAATTTCATCTGGTAA+AGG + chr2_1:1659510-1659529 Msa0186910:CDS 35.0%
CTGGTAAAGGTAAAAAGGAT+AGG + chr2_1:1659523-1659542 Msa0186910:CDS 35.0%
TTTGTTGAGACGTTTGCAAT+TGG + chr2_1:1659630-1659649 Msa0186910:CDS 35.0%
TTGTTGAGACGTTTGCAATT+GGG + chr2_1:1659631-1659650 Msa0186910:CDS 35.0%
TTTCACCTATTGCTCAGATT+TGG + chr2_1:1659706-1659725 Msa0186910:CDS 35.0%
GAATTCCAAATCTGAGCAAT+AGG - chr2_1:1659714-1659733 Msa0186920:three_prime_UTR 35.0%
GACAATAAAACAAACTGCGA+TGG - chr2_1:1659006-1659025 Msa0186920:intron 35.0%
! GAATTGTTGTTGCTTCACAA+TGG + chr2_1:1659069-1659088 Msa0186910:intron 35.0%
CTCCCATTTCAAAATAAAGG+AGG - chr2_1:1659100-1659119 Msa0186920:intron 35.0%
!!! CTCCTTTATTTTGAAATGGG+AGG + chr2_1:1659098-1659117 Msa0186910:intron 35.0%
CCAAAAAAACCAGTCTCTAA+CGG + chr2_1:1659149-1659168 Msa0186910:intron 35.0%
ATTGAAATGTACCAGTAGAC+AGG - chr2_1:1659208-1659227 Msa0186920:intron 35.0%
! TATAGAAGGTACATTTGGCT+TGG - chr2_1:1659352-1659371 Msa0186920:three_prime_UTR 35.0%
! TCAGGTATAGAAGGTACATT+TGG - chr2_1:1659357-1659376 Msa0186920:three_prime_UTR 35.0%
GGAGTAATTTCATCTGGTAA+AGG + chr2_1:1659510-1659529 Msa0186910:CDS 35.0%
CTGGTAAAGGTAAAAAGGAT+AGG + chr2_1:1659523-1659542 Msa0186910:CDS 35.0%
TTTGTTGAGACGTTTGCAAT+TGG + chr2_1:1659630-1659649 Msa0186910:CDS 35.0%
TTGTTGAGACGTTTGCAATT+GGG + chr2_1:1659631-1659650 Msa0186910:CDS 35.0%
TTTCACCTATTGCTCAGATT+TGG + chr2_1:1659706-1659725 Msa0186910:CDS 35.0%
GAATTCCAAATCTGAGCAAT+AGG - chr2_1:1659714-1659733 Msa0186920:three_prime_UTR 35.0%
!!! CAGCTTTTGATTTTGCCTTG+CGG - chr2_1:1658754-1658773 Msa0186920:three_prime_UTR 40.0%
!!! CCGTTAGAGACTGGTTTTTT+TGG - chr2_1:1659152-1659171 Msa0186920:intron 40.0%
AAAGAAGATTCCTTCGCCAA+AGG + chr2_1:1659401-1659420 Msa0186910:CDS 40.0%
GATATCAATTCCTTTGGCGA+AGG - chr2_1:1659414-1659433 Msa0186920:three_prime_UTR 40.0%
TTCACAAGAAGCCTCATTGA+AGG + chr2_1:1659458-1659477 Msa0186910:CDS 40.0%
ATCCTCTATGACCTTCAATG+AGG - chr2_1:1659472-1659491 Msa0186920:three_prime_UTR 40.0%
GGTCATAGAGGATTTACAGA+AGG + chr2_1:1659479-1659498 Msa0186910:CDS 40.0%
!! GATTTACAGAAGGCGTTGTT+TGG + chr2_1:1659489-1659508 Msa0186910:CDS 40.0%
ATGAAGCTTGATACTAAGCC+TGG + chr2_1:1659606-1659625 Msa0186910:CDS 40.0%
TGTTGAGACGTTTGCAATTG+GGG + chr2_1:1659632-1659651 Msa0186910:CDS 40.0%
! TTTGCAATTGGGGTTGTTTC+TGG + chr2_1:1659642-1659661 Msa0186910:CDS 40.0%
GCAATAGGTGAAAGAATGTG+AGG - chr2_1:1659699-1659718 Msa0186920:three_prime_UTR 40.0%
TCATTGAGGGGTAGATTTAG+TGG - chr2_1:1659748-1659767 Msa0186920:three_prime_UTR 40.0%
GGGTACAAAATGTCATTGAG+GGG - chr2_1:1659760-1659779 Msa0186920:three_prime_UTR 40.0%
!! CTTCCTATGCTCTGCTTTTA+GGG - chr2_1:1658696-1658715 Msa0186920:three_prime_UTR 40.0%
!! TCTTCCTATGCTCTGCTTTT+AGG - chr2_1:1658697-1658716 Msa0186920:three_prime_UTR 40.0%
!!! CAGCTTTTGATTTTGCCTTG+CGG - chr2_1:1658754-1658773 Msa0186920:three_prime_UTR 40.0%
!!! CCGTTAGAGACTGGTTTTTT+TGG - chr2_1:1659152-1659171 Msa0186920:intron 40.0%
AAAGAAGATTCCTTCGCCAA+AGG + chr2_1:1659401-1659420 Msa0186910:CDS 40.0%
GATATCAATTCCTTTGGCGA+AGG - chr2_1:1659414-1659433 Msa0186920:three_prime_UTR 40.0%
TTCACAAGAAGCCTCATTGA+AGG + chr2_1:1659458-1659477 Msa0186910:CDS 40.0%
ATCCTCTATGACCTTCAATG+AGG - chr2_1:1659472-1659491 Msa0186920:three_prime_UTR 40.0%
GGTCATAGAGGATTTACAGA+AGG + chr2_1:1659479-1659498 Msa0186910:CDS 40.0%
!! GATTTACAGAAGGCGTTGTT+TGG + chr2_1:1659489-1659508 Msa0186910:CDS 40.0%
ATGAAGCTTGATACTAAGCC+TGG + chr2_1:1659606-1659625 Msa0186910:CDS 40.0%
TGTTGAGACGTTTGCAATTG+GGG + chr2_1:1659632-1659651 Msa0186910:CDS 40.0%
! TTTGCAATTGGGGTTGTTTC+TGG + chr2_1:1659642-1659661 Msa0186910:CDS 40.0%
GCAATAGGTGAAAGAATGTG+AGG - chr2_1:1659699-1659718 Msa0186920:three_prime_UTR 40.0%
TCATTGAGGGGTAGATTTAG+TGG - chr2_1:1659748-1659767 Msa0186920:three_prime_UTR 40.0%
GGGTACAAAATGTCATTGAG+GGG - chr2_1:1659760-1659779 Msa0186920:three_prime_UTR 40.0%
! TTGATTTTGCCTTGCGGCTA+GGG - chr2_1:1658748-1658767 Msa0186920:three_prime_UTR 45.0%
!! TTTGATTTTGCCTTGCGGCT+AGG - chr2_1:1658749-1658768 Msa0186920:three_prime_UTR 45.0%
GCTTTCGTTGACACGTACTT+CGG - chr2_1:1658906-1658925 Msa0186920:intron 45.0%
GAAATGTACCAGTAGACAGG+TGG - chr2_1:1659205-1659224 Msa0186920:intron 45.0%
AGCCTCATTGAAGGTCATAG+AGG + chr2_1:1659467-1659486 Msa0186910:CDS 45.0%
GCAAACGTCTCAACAAATCC+AGG - chr2_1:1659627-1659646 Msa0186920:three_prime_UTR 45.0%
!! GTTTCTGGTGCTGCTCTTAA+TGG + chr2_1:1659657-1659676 Msa0186910:CDS 45.0%
!! GGTGCTGCTCTTAATGGAAT+TGG + chr2_1:1659663-1659682 Msa0186910:CDS 45.0%
! TTGATTTTGCCTTGCGGCTA+GGG - chr2_1:1658748-1658767 Msa0186920:three_prime_UTR 45.0%
!! TTTGATTTTGCCTTGCGGCT+AGG - chr2_1:1658749-1658768 Msa0186920:three_prime_UTR 45.0%
GCTTTCGTTGACACGTACTT+CGG - chr2_1:1658906-1658925 Msa0186920:intron 45.0%
GAAATGTACCAGTAGACAGG+TGG - chr2_1:1659205-1659224 Msa0186920:intron 45.0%
AGCCTCATTGAAGGTCATAG+AGG + chr2_1:1659467-1659486 Msa0186910:CDS 45.0%
GCAAACGTCTCAACAAATCC+AGG - chr2_1:1659627-1659646 Msa0186920:three_prime_UTR 45.0%
!! GTTTCTGGTGCTGCTCTTAA+TGG + chr2_1:1659657-1659676 Msa0186910:CDS 45.0%
!! GGTGCTGCTCTTAATGGAAT+TGG + chr2_1:1659663-1659682 Msa0186910:CDS 45.0%
TACACTGAGCCGTTAGAGAC+TGG - chr2_1:1659161-1659180 Msa0186920:intron 50.0%
AGGGGTAGATTTAGTGGCTG+AGG - chr2_1:1659742-1659761 Msa0186920:three_prime_UTR 50.0%
TACACTGAGCCGTTAGAGAC+TGG - chr2_1:1659161-1659180 Msa0186920:intron 50.0%
AGGGGTAGATTTAGTGGCTG+AGG - chr2_1:1659742-1659761 Msa0186920:three_prime_UTR 50.0%
AGCGAAAAACCCTAGCCGCA+AGG + chr2_1:1658736-1658755 Msa0186910:five_prime_UTR 55.0%
ACACTACGCCACCTGTCTAC+TGG + chr2_1:1659194-1659213 Msa0186910:intron 55.0%
AGCGAAAAACCCTAGCCGCA+AGG + chr2_1:1658736-1658755 Msa0186910:five_prime_UTR 55.0%
ACACTACGCCACCTGTCTAC+TGG + chr2_1:1659194-1659213 Msa0186910:intron 55.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 1658696 1659779 1658696 ID=Msa0186910;Name=Msa0186910
chr2_1 mRNA 1658696 1659779 1658696 ID=Msa0186910-mRNA-1;Parent=Msa0186910;Name=Msa0186910-mRNA-1;_AED=0.00;_eAED=0.00;_QI=90|1|1|1|0|0.5|2|12|146
chr2_1 exon 1658696 1658786 1658696 ID=Msa0186910-mRNA-1:exon:676;Parent=Msa0186910-mRNA-1
chr2_1 exon 1659328 1659779 1659328 ID=Msa0186910-mRNA-1:exon:677;Parent=Msa0186910-mRNA-1
chr2_1 five_prime_UTR 1658696 1658785 1658696 ID=Msa0186910-mRNA-1:five_prime_utr;Parent=Msa0186910-mRNA-1
chr2_1 CDS 1658786 1658786 1658786 ID=Msa0186910-mRNA-1:cds;Parent=Msa0186910-mRNA-1
chr2_1 CDS 1659328 1659767 1659328 ID=Msa0186910-mRNA-1:cds;Parent=Msa0186910-mRNA-1
chr2_1 three_prime_UTR 1659768 1659779 1659768 ID=Msa0186910-mRNA-1:three_prime_utr;Parent=Msa0186910-mRNA-1
Gene Sequence

>Msa0186910

ATGGTTGAAATGGAACAATCTTCCAAGCCAAATGTACCTTCTATACCTGAAATCAAAATGAAACAAAATCAGAAAAAGAAGATTCCTTCGCCAAAGGAATTGATATCCTATTATGAATCACAAGGAATGGATTCACAAGAAGCCTCATTGAAGGTCATAGAGGATTTACAGAAGGCGTTGTTTGGAGTAATTTCATCTGGTAAAGGTAAAAAGGATAGGCTTATAAATGAATCTTCTAGAAAGATGGATTCGGTTAATCAAAGACTTGCTATACTTGATATGAAGCTTGATACTAAGCCTGGATTTGTTGAGACGTTTGCAATTGGGGTTGTTTCTGGTGCTGCTCTTAATGGAATTGGTGCTATAATGCCTCACATTCTTTCACCTATTGCTCAGATTTGGAATTCTGTTACCTCAGCCACTAAATCTACCCCTCAATGA
Protein sequence

>Msa0186910

MVEMEQSSKPNVPSIPEIKMKQNQKKKIPSPKELISYYESQGMDSQEASLKVIEDLQKALFGVISSGKGKKDRLINESSRKMDSVNQRLAILDMKLDTKPGFVETFAIGVVSGAALNGIGAIMPHILSPIAQIWNSVTSATKSTPQ*