AlfalfaGEDB | Msa0715650

M. sativa cultivar zhongmu-4 / Msa0715650

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0715650	XP_003611849.1	95.109	184	9	0	1	184	1	184	1.50e-119	346

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0715650	G7K9G4	95.109	184	9	0	1	184	1	184	7.15e-120	346

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0006040	Msa0715650	0.802982	4.332131e-49	-8.615850e-47
Msa0006290	Msa0715650	0.819951	8.868295e-53	-8.615850e-47
Msa0011960	Msa0715650	0.850448	1.686643e-60	-8.615850e-47
Msa0019390	Msa0715650	0.860090	2.630864e-63	-8.615850e-47
Msa0021220	Msa0715650	0.804206	2.414420e-49	-8.615850e-47
Msa0022970	Msa0715650	0.803153	3.992809e-49	-8.615850e-47
Msa0025730	Msa0715650	0.855798	4.953250e-62	-8.615850e-47
Msa0027260	Msa0715650	0.809533	1.805641e-50	-8.615850e-47
Msa0027770	Msa0715650	0.819348	1.217689e-52	-8.615850e-47
Msa0029260	Msa0715650	0.823720	1.190020e-53	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0715650	MtrunA17_Chr5g0403031	95.109	184	9	0	1	184	1	184	1.38e-123	346

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0715650	AT4G37090.1	45.946	185	88	4	1	180	1	178	5.91e-33	116
Msa0715650	AT4G37090.2	45.946	185	86	5	1	180	1	176	1.92e-26	99.8

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Find 44 sgRNAs with CRISPR-Local

Find 189 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGAGAAAATCCAAGGATTC+AGG	0.267478	5_1:-8757947	Msa0715650:CDS
AAGCATTTCAACAATGCTTT+TGG	0.238659	5_1:+8758129	None:intergenic
AAGGCAGTCTTTAAGCATTC+AGG	0.411790	5_1:+8757789	None:intergenic
AAGTAGACGTAATGCTTGGT+TGG	0.572908	5_1:+8757707	None:intergenic
AATTAAAGGCTACCACTTCA+AGG	0.462256	5_1:-8757970	Msa0715650:CDS
AGAAAGAACGTCGGCGAAAA+TGG	0.201521	5_1:-8758279	Msa0715650:CDS
AGAAATCCCACAGCAGTGTT+TGG	0.406449	5_1:+8758154	None:intergenic
AGAGAAAATCCAAGGATTCA+GGG	0.497993	5_1:-8757946	Msa0715650:intron
AGAGATTCGAAAGATTAAAA+AGG	0.343332	5_1:-8759461	Msa0715650:CDS
AGGACAGGATATATAGGAAG+TGG	0.516185	5_1:-8756710	Msa0715650:intron

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CAAAATCAGGTTTAGTTATG+AGG	-	chr5_1:8756925-8756944	Msa0715650:intron	30.0%
GATATTGATCAATCTTGATG+TGG	-	chr5_1:8757118-8757137	Msa0715650:intron	30.0%
TGCAGTATAAGAGAGAATAA+AGG	+	chr5_1:8757945-8757964	None:intergenic	30.0%
TAATATGACTTTACATGGCA+GGG	-	chr5_1:8758045-8758064	Msa0715650:intron	30.0%
ATATTGAAGAGAAAGAACGT+CGG	-	chr5_1:8758071-8758090	Msa0715650:intron	30.0%
CCAAAGCTACTAAAAAATCT+AGG	-	chr5_1:8758128-8758147	Msa0715650:CDS	30.0%
TTTATTAACTGAGACTGATG+AGG	-	chr5_1:8758357-8758376	Msa0715650:intron	30.0%
TGAGGAGGAAGATAAATTAA+AGG	-	chr5_1:8758375-8758394	Msa0715650:intron	30.0%
GGAATAAAAATCCTCATTTC+TGG	+	chr5_1:8758698-8758717	None:intergenic	30.0%
GGAATGATAACACTGATAAA+AGG	-	chr5_1:8758924-8758943	Msa0715650:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_1	gene	8756628	8759753	8756628	ID=Msa0715650;Name=Msa0715650
chr5_1	mRNA	8756628	8759753	8756628	ID=Msa0715650-mRNA-1;Parent=Msa0715650;Name=Msa0715650-mRNA-1;_AED=0.21;_eAED=0.21;_QI=74\|1\|1\|1\|0.8\|0.66\|6\|141\|184
chr5_1	exon	8756628	8756725	8756628	ID=Msa0715650-mRNA-1:exon:3629;Parent=Msa0715650-mRNA-1
chr5_1	exon	8757634	8757845	8757634	ID=Msa0715650-mRNA-1:exon:3628;Parent=Msa0715650-mRNA-1
chr5_1	exon	8757947	8758030	8757947	ID=Msa0715650-mRNA-1:exon:3627;Parent=Msa0715650-mRNA-1
chr5_1	exon	8758115	8758315	8758115	ID=Msa0715650-mRNA-1:exon:3626;Parent=Msa0715650-mRNA-1
chr5_1	exon	8759448	8759500	8759448	ID=Msa0715650-mRNA-1:exon:3625;Parent=Msa0715650-mRNA-1
chr5_1	exon	8759632	8759753	8759632	ID=Msa0715650-mRNA-1:exon:3624;Parent=Msa0715650-mRNA-1
chr5_1	five_prime_UTR	8759680	8759753	8759680	ID=Msa0715650-mRNA-1:five_prime_utr;Parent=Msa0715650-mRNA-1
chr5_1	CDS	8759632	8759679	8759632	ID=Msa0715650-mRNA-1:cds;Parent=Msa0715650-mRNA-1
chr5_1	CDS	8759448	8759500	8759448	ID=Msa0715650-mRNA-1:cds;Parent=Msa0715650-mRNA-1
chr5_1	CDS	8758115	8758315	8758115	ID=Msa0715650-mRNA-1:cds;Parent=Msa0715650-mRNA-1
chr5_1	CDS	8757947	8758030	8757947	ID=Msa0715650-mRNA-1:cds;Parent=Msa0715650-mRNA-1
chr5_1	CDS	8757677	8757845	8757677	ID=Msa0715650-mRNA-1:cds;Parent=Msa0715650-mRNA-1
chr5_1	three_prime_UTR	8757634	8757676	8757634	ID=Msa0715650-mRNA-1:three_prime_utr;Parent=Msa0715650-mRNA-1
chr5_1	three_prime_UTR	8756628	8756725	8756628	ID=Msa0715650-mRNA-1:three_prime_utr;Parent=Msa0715650-mRNA-1

Gene Sequence

>Msa0715650

ATGTCAGACAACGATTCTGATGCCCCTGAGGAATTCACAGCTGAACAGGGTATTCAGCTAGACGAAGAGATTCGAAAGATTAAAAAGGAAAACAAAATCAGGGTTAATATTGAAGAGAAAGAACGTCGGCGAAAATGGGCTCAAAATTTAACACCTCGACCATCCAAAGCTACTAAAAAATCTAGGGACGCATCAAACACTGAATCTGAGCAAGAACCAAACACTGAATCTCAGCAAAAACCAAACACTGCTGTGGGATTTCTTCCCAAAAGCATTGTTGAAATGCTTGCAGCTAAGGAGAAAAAAAATTTATTAACTGAGACTGATGAGGAGGAAGATAAATTAAAGGCTACCACTTCAAGGAAGAGAAAATCCAAGGATTCAGGTTCTGAACCTATTATTGTGAGCAAACCAGCTCCTCCTGAATGCTTAAAGACTGCCTTGGATTTCTTGAAAGAAAGGAAAATGTCAGTTCCAAGATCGTCTGCTGTTTTGAAGAATTCCAACCAAGCATTACGTCTACTTTCCAGTTCCGGGATCATACGCCAGAAATGA

Protein sequence

>Msa0715650

MSDNDSDAPEEFTAEQGIQLDEEIRKIKKENKIRVNIEEKERRRKWAQNLTPRPSKATKKSRDASNTESEQEPNTESQQKPNTAVGFLPKSIVEMLAAKEKKNLLTETDEEEDKLKATTSRKRKSKDSGSEPIIVSKPAPPECLKTALDFLKERKMSVPRSSAVLKNSNQALRLLSSSGIIRQK*