AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0192350

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0192350 MtrunA17_Chr3g0118291 100.000 204 0 0 1 204 1 204 6.78e-150 414
Msa0192350 MtrunA17_Chr2g0282541 99.510 204 1 0 1 204 49 252 1.89e-149 415
Msa0192350 MtrunA17_Chr7g0224171 90.323 62 6 0 1 62 1 62 1.93e-35 120
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0192350 AT4G16720.1 88.235 204 24 0 1 204 1 204 9.00e-122 343
Msa0192350 AT4G17390.1 87.745 204 25 0 1 204 1 204 1.88e-121 342

Find 97 sgRNAs with CRISPR-Local

Find 247 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGATAGTTTGAGAATTCTTT+AGG 0.240913 2_1:+8118655 Msa0192350:three_prime_UTR
CGTGAGACAAACCGAGAAAA+TGG 0.255682 2_1:+8117159 Msa0192350:exon
GTTTCGAAGGGTATTGTATA+CGG 0.299480 2_1:+8117723 Msa0192350:CDS
GGAGGAAGCTCGGTGGCCTC+AGG 0.313744 2_1:+8117820 Msa0192350:CDS
TCCTGACAAAGCTCGCCGTT+TGG 0.353079 2_1:+8117421 Msa0192350:CDS
GATAGTTTGAGAATTCTTTA+GGG 0.355332 2_1:+8118656 Msa0192350:three_prime_UTR
TCAGGGTTCTCAATTCCTAC+TGG 0.363571 2_1:+8117838 Msa0192350:CDS
ACAAGTATGTTTCTGAGTTA+TGG 0.369598 2_1:+8117300 Msa0192350:CDS
CCTGACAAAGCTCGCCGTTT+GGG 0.382164 2_1:+8117422 Msa0192350:CDS
GTCCTTCCCGCAGGGCAAAC+TGG 0.382984 2_1:+8118054 Msa0192350:CDS
AGTGCATTATATTTAGACTT+TGG 0.387924 2_1:+8118159 Msa0192350:three_prime_UTR
AGGTTCTTATCTCTGGTGTC+TGG 0.388442 2_1:+8118562 Msa0192350:three_prime_UTR
TTAGTGAGCCAGTTGATTCT+TGG 0.391426 2_1:-8117936 None:intergenic
TAGTGAGCCAGTTGATTCTT+GGG 0.399274 2_1:-8117935 None:intergenic
TTTGTCTCACGCTGGCTGGC+AGG 0.404631 2_1:-8117147 None:intergenic
TGCGGGAAGGACGGGCTTTG+TGG 0.410020 2_1:-8118043 None:intergenic
TCTCTTCCAGTTTGCCCTGC+GGG 0.416755 2_1:-8118060 None:intergenic
AATGATGAGGTTCTTATCTC+TGG 0.417708 2_1:+8118555 Msa0192350:three_prime_UTR
AATGACCTCAAAATACTTGA+AGG 0.426532 2_1:-8117877 None:intergenic
AGCTCGCCGTTTGGGTTACA+AGG 0.427870 2_1:+8117430 Msa0192350:CDS
CCCAAACGGCGAGCTTTGTC+AGG 0.428600 2_1:-8117422 None:intergenic
TCGGTTTGTCTCACGCTGGC+TGG 0.439091 2_1:-8117151 None:intergenic
TATACAATACCCTTCGAAAC+AGG 0.440190 2_1:-8117720 None:intergenic
GTTTGCCCTGCGGGAAGGAC+GGG 0.445042 2_1:-8118051 None:intergenic
TTTGTCAGGACGAGTGGGTC+TGG 0.461552 2_1:-8117408 None:intergenic
AGTTTGCCCTGCGGGAAGGA+CGG 0.463498 2_1:-8118052 None:intergenic
CAGTAACACCTGCTCTCATT+TGG 0.466095 2_1:+8118436 Msa0192350:three_prime_UTR
TTGAGTAACTCCCTGGTTAG+TGG 0.468290 2_1:-8117751 None:intergenic
CTTCGTGGCCTCACTTCTGC+TGG 0.468828 2_1:+8117981 Msa0192350:CDS
TCAGTTGCCGAGGAGCGTGC+TGG 0.471507 2_1:+8117798 Msa0192350:CDS
TGAGTAACTCCCTGGTTAGT+GGG 0.482971 2_1:-8117750 None:intergenic
CTAATCCTGTCCACAAGCAT+AGG 0.483564 2_1:+8117955 Msa0192350:CDS
CAGTTGCCGAGGAGCGTGCT+GGG 0.483968 2_1:+8117799 Msa0192350:CDS
CAGGGTTCTCAATTCCTACT+GGG 0.490551 2_1:+8117839 Msa0192350:CDS
TCATGCAGCGTGTGAGGTGT+TGG 0.501162 2_1:+8117351 Msa0192350:CDS
ACAAAGCCCGTCCTTCCCGC+AGG 0.505243 2_1:+8118045 Msa0192350:CDS
CAACAATTCCAAACTATGCT+AGG 0.505974 2_1:-8118737 None:intergenic
CATGCAGCGTGTGAGGTGTT+GGG 0.510206 2_1:+8117352 Msa0192350:CDS
GATTATTCTAATGAAGCCAA+TGG 0.510423 2_1:+8118696 Msa0192350:three_prime_UTR
GAGCGTGCTGGGAGGAAGCT+CGG 0.519651 2_1:+8117810 Msa0192350:CDS
GTGAGACAAACCGAGAAAAT+GGG 0.520353 2_1:+8117160 Msa0192350:exon
TACGTAGAGGTGGTAGGAAG+AGG 0.522230 2_1:+8117697 Msa0192350:CDS
TTCCAGTTTGCCCTGCGGGA+AGG 0.525009 2_1:-8118056 None:intergenic
TTACTCAACTGAAGTTTCAA+AGG 0.527187 2_1:+8117766 Msa0192350:CDS
AACAGAGGTTTGAGTGGCAA+AGG 0.530737 2_1:+8118011 Msa0192350:CDS
TTTCGAAGGGTATTGTATAC+GGG 0.532253 2_1:+8117724 Msa0192350:CDS
CTTCCTCCCAGCACGCTCCT+CGG 0.535254 2_1:-8117805 None:intergenic
TTTGGGTTACAAGGCCAAGC+AGG 0.540516 2_1:+8117439 Msa0192350:CDS
AGTTCCCTATGCTTGTGGAC+AGG 0.542094 2_1:-8117960 None:intergenic
ATACGGGAAGCCCACTAACC+AGG 0.546286 2_1:+8117740 Msa0192350:CDS
GCGAGCTTTGTCAGGACGAG+TGG 0.547527 2_1:-8117414 None:intergenic
CACCTCTACGTACCCTCACT+CGG 0.548557 2_1:-8117686 None:intergenic
AAGAATAGATGATACCTGCT+TGG 0.550130 2_1:-8117453 None:intergenic
GAAATGACCCAAGAATCAAC+TGG 0.550461 2_1:+8117928 Msa0192350:CDS
TAGGAAGAGGCCTGTTTCGA+AGG 0.550472 2_1:+8117710 Msa0192350:CDS
GAGGAAGCTCGGTGGCCTCA+GGG 0.552741 2_1:+8117821 Msa0192350:CDS
TTGTTATTAGTAGAGGTGGA+TGG 0.554620 2_1:+8118634 Msa0192350:three_prime_UTR
ACTTCAGTTGAGTAACTCCC+TGG 0.559985 2_1:-8117758 None:intergenic
CACGAAGTTCCCTATGCTTG+TGG 0.560507 2_1:-8117965 None:intergenic
TAATCCTGTCCACAAGCATA+GGG 0.571077 2_1:+8117956 Msa0192350:CDS
TTCTAGGCAAAGACACAGAT+CGG 0.576700 2_1:-8118210 None:intergenic
CAATTCCTACTGGGTCAATG+AGG 0.583661 2_1:+8117848 Msa0192350:CDS
TTTGACAGCCAAATGAGAGC+AGG 0.586910 2_1:-8118444 None:intergenic
TTCTCTTCCAGTTTGCCCTG+CGG 0.587901 2_1:-8118061 None:intergenic
GATATGTTGTGTACCGAGTG+AGG 0.591649 2_1:+8117673 Msa0192350:CDS
CTTTGTTGTTTGGATTGACA+CGG 0.593413 2_1:+8118347 Msa0192350:three_prime_UTR
TGAGGGTACGTAGAGGTGGT+AGG 0.593507 2_1:+8117691 Msa0192350:CDS
ACAGAGGTTTGAGTGGCAAA+GGG 0.593708 2_1:+8118012 Msa0192350:CDS
GCTTGGCCTTGTAACCCAAA+CGG 0.594502 2_1:-8117436 None:intergenic
GAGCAAGAGATCAGTTGCCG+AGG 0.598712 2_1:+8117788 Msa0192350:CDS
CACAAGCATAGGGAACTTCG+TGG 0.601845 2_1:+8117966 Msa0192350:CDS
CAAAGCCCGTCCTTCCCGCA+GGG 0.606273 2_1:+8118046 Msa0192350:CDS
TTTCTCGGTTTGTCTCACGC+TGG 0.609610 2_1:-8117155 None:intergenic
AGGAAGAGGCCTGTTTCGAA+GGG 0.612480 2_1:+8117711 Msa0192350:CDS
TACGGGAAGCCCACTAACCA+GGG 0.619308 2_1:+8117741 Msa0192350:CDS
AGAATCCTCATTGACCCAGT+AGG 0.619890 2_1:-8117853 None:intergenic
AAAGAACAAGTGATCAAACA+AGG 0.621736 2_1:-8118277 None:intergenic
AAAACGGTAAACTATGCTCA+AGG 0.622386 2_1:+8118522 Msa0192350:three_prime_UTR
CGAGCTTTGTCAGGACGAGT+GGG 0.623786 2_1:-8117413 None:intergenic
GGGTCTGGTGAGACGAACAA+TGG 0.629081 2_1:-8117393 None:intergenic
ACGATAACGACGGAGAGACA+AGG 0.632844 2_1:-8118090 None:intergenic
AAAGAAAACAGAGGTTTGAG+TGG 0.633735 2_1:+8118005 Msa0192350:CDS
AGGCACATTGATGAATGATG+AGG 0.635465 2_1:+8118542 Msa0192350:three_prime_UTR
TGAGATTCATGCAGCGTGTG+AGG 0.638236 2_1:+8117345 Msa0192350:CDS
AGTATGTTTCTGAGTTATGG+CGG 0.642445 2_1:+8117303 Msa0192350:CDS
CGAGTGAGGGTACGTAGAGG+TGG 0.643734 2_1:+8117687 Msa0192350:CDS
ATATGTTGTGTACCGAGTGA+GGG 0.647122 2_1:+8117674 Msa0192350:CDS
TATTCTAATGAAGCCAATGG+TGG 0.652154 2_1:+8118699 Msa0192350:three_prime_UTR
GTCATGTAGCTATCAAGTGA+TGG 0.658852 2_1:+8118484 Msa0192350:three_prime_UTR
TCTGCTGGAAAAGAAAACAG+AGG 0.661682 2_1:+8117996 Msa0192350:CDS
GTTATGGCGGAAGAAGCAAT+CGG 0.676275 2_1:+8117316 Msa0192350:CDS
TTGCCGAGGAGCGTGCTGGG+AGG 0.682144 2_1:+8117802 Msa0192350:CDS
CGTGCTGGGAGGAAGCTCGG+TGG 0.684204 2_1:+8117813 Msa0192350:CDS
AAGAACAAGTGATCAAACAA+GGG 0.687485 2_1:-8118276 None:intergenic
AGTAAAATCAACGATAACGA+CGG 0.699472 2_1:-8118100 None:intergenic
TACCGAGTGAGGGTACGTAG+AGG 0.717794 2_1:+8117684 Msa0192350:CDS
AGTAGGAATTGAGAACCCTG+AGG 0.790898 2_1:-8117836 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTTATTTGTGAATTTTGAAT+AGG + chr2_1:8117272-8117291 Msa0192350:intron 20.0%
!! CATTAAGTGTGAAATAATTA+GGG - chr2_1:8117576-8117595 None:intergenic 20.0%
!!! AGTATTTGTTACATCATTTT+AGG + chr2_1:8117650-8117669 Msa0192350:intron 20.0%
!!! GTATTTGTTACATCATTTTA+GGG + chr2_1:8117651-8117670 Msa0192350:intron 20.0%
!!! TTCATTTTTGTTATTAGTAG+AGG + chr2_1:8118627-8118646 Msa0192350:three_prime_UTR 20.0%
!!! GTTATTTGTGAATTTTGAAT+AGG + chr2_1:8117272-8117291 Msa0192350:intron 20.0%
!! CATTAAGTGTGAAATAATTA+GGG - chr2_1:8117576-8117595 None:intergenic 20.0%
!!! AGTATTTGTTACATCATTTT+AGG + chr2_1:8117650-8117669 Msa0192350:intron 20.0%
!!! GTATTTGTTACATCATTTTA+GGG + chr2_1:8117651-8117670 Msa0192350:intron 20.0%
!!! TTCATTTTTGTTATTAGTAG+AGG + chr2_1:8118627-8118646 Msa0192350:three_prime_UTR 20.0%
! GCATTAAGTGTGAAATAATT+AGG - chr2_1:8117577-8117596 None:intergenic 25.0%
! AGTGCATTATATTTAGACTT+TGG + chr2_1:8118159-8118178 Msa0192350:three_prime_UTR 25.0%
! AACAACAAAAATGAGTTTCT+AGG - chr2_1:8118229-8118248 None:intergenic 25.0%
!!! ATTTTTGTTATTAGTAGAGG+TGG + chr2_1:8118630-8118649 Msa0192350:three_prime_UTR 25.0%
! GATAGTTTGAGAATTCTTTA+GGG + chr2_1:8118656-8118675 Msa0192350:three_prime_UTR 25.0%
!! GAAGCAATTTTTGTTAAAAC+TGG + chr2_1:8118801-8118820 Msa0192350:three_prime_UTR 25.0%
! GCATTAAGTGTGAAATAATT+AGG - chr2_1:8117577-8117596 None:intergenic 25.0%
! AGTGCATTATATTTAGACTT+TGG + chr2_1:8118159-8118178 Msa0192350:three_prime_UTR 25.0%
! AACAACAAAAATGAGTTTCT+AGG - chr2_1:8118229-8118248 None:intergenic 25.0%
!!! ATTTTTGTTATTAGTAGAGG+TGG + chr2_1:8118630-8118649 Msa0192350:three_prime_UTR 25.0%
! GATAGTTTGAGAATTCTTTA+GGG + chr2_1:8118656-8118675 Msa0192350:three_prime_UTR 25.0%
! ACAAGTATGTTTCTGAGTTA+TGG + chr2_1:8117300-8117319 Msa0192350:CDS 30.0%
TGAAATAATTAGGGTCTGTT+TGG - chr2_1:8117567-8117586 None:intergenic 30.0%
TTTCACACTTAATGCTATGT+AGG + chr2_1:8117582-8117601 Msa0192350:intron 30.0%
TTACTCAACTGAAGTTTCAA+AGG + chr2_1:8117766-8117785 Msa0192350:CDS 30.0%
! TTCTACCTTCAAGTATTTTG+AGG + chr2_1:8117872-8117891 Msa0192350:CDS 30.0%
AATGACCTCAAAATACTTGA+AGG - chr2_1:8117880-8117899 None:intergenic 30.0%
!!! CAAGTATTTTGAGGTCATTT+TGG + chr2_1:8117881-8117900 Msa0192350:CDS 30.0%
AGTAAAATCAACGATAACGA+CGG - chr2_1:8118103-8118122 None:intergenic 30.0%
AAGAACAAGTGATCAAACAA+GGG - chr2_1:8118279-8118298 None:intergenic 30.0%
AAAGAACAAGTGATCAAACA+AGG - chr2_1:8118280-8118299 None:intergenic 30.0%
!!! CTATGTTTTGCTTTGTTGTT+TGG + chr2_1:8118337-8118356 Msa0192350:three_prime_UTR 30.0%
! GTTCTTTTGTAGCTTCAAAA+CGG + chr2_1:8118506-8118525 Msa0192350:three_prime_UTR 30.0%
GGATAGTTTGAGAATTCTTT+AGG + chr2_1:8118655-8118674 Msa0192350:three_prime_UTR 30.0%
GATTATTCTAATGAAGCCAA+TGG + chr2_1:8118696-8118715 Msa0192350:three_prime_UTR 30.0%
AAAAATAGCCTAGCATAGTT+TGG + chr2_1:8118729-8118748 Msa0192350:three_prime_UTR 30.0%
! ACAAGTATGTTTCTGAGTTA+TGG + chr2_1:8117300-8117319 Msa0192350:CDS 30.0%
TGAAATAATTAGGGTCTGTT+TGG - chr2_1:8117567-8117586 None:intergenic 30.0%
TTTCACACTTAATGCTATGT+AGG + chr2_1:8117582-8117601 Msa0192350:intron 30.0%
TTACTCAACTGAAGTTTCAA+AGG + chr2_1:8117766-8117785 Msa0192350:CDS 30.0%
! TTCTACCTTCAAGTATTTTG+AGG + chr2_1:8117872-8117891 Msa0192350:CDS 30.0%
AATGACCTCAAAATACTTGA+AGG - chr2_1:8117880-8117899 None:intergenic 30.0%
!!! CAAGTATTTTGAGGTCATTT+TGG + chr2_1:8117881-8117900 Msa0192350:CDS 30.0%
AGTAAAATCAACGATAACGA+CGG - chr2_1:8118103-8118122 None:intergenic 30.0%
AAGAACAAGTGATCAAACAA+GGG - chr2_1:8118279-8118298 None:intergenic 30.0%
AAAGAACAAGTGATCAAACA+AGG - chr2_1:8118280-8118299 None:intergenic 30.0%
!!! CTATGTTTTGCTTTGTTGTT+TGG + chr2_1:8118337-8118356 Msa0192350:three_prime_UTR 30.0%
! GTTCTTTTGTAGCTTCAAAA+CGG + chr2_1:8118506-8118525 Msa0192350:three_prime_UTR 30.0%
GGATAGTTTGAGAATTCTTT+AGG + chr2_1:8118655-8118674 Msa0192350:three_prime_UTR 30.0%
GATTATTCTAATGAAGCCAA+TGG + chr2_1:8118696-8118715 Msa0192350:three_prime_UTR 30.0%
AAAAATAGCCTAGCATAGTT+TGG + chr2_1:8118729-8118748 Msa0192350:three_prime_UTR 30.0%
!! CTGTTTCTATTCATTCTCTC+TGG + chr2_1:8117206-8117225 Msa0192350:intron 35.0%
! AGTATGTTTCTGAGTTATGG+CGG + chr2_1:8117303-8117322 Msa0192350:CDS 35.0%
AAGAATAGATGATACCTGCT+TGG - chr2_1:8117456-8117475 None:intergenic 35.0%
TAATTAGGGTCTGTTTGGAT+TGG - chr2_1:8117562-8117581 None:intergenic 35.0%
TATACAATACCCTTCGAAAC+AGG - chr2_1:8117723-8117742 None:intergenic 35.0%
!! GTTTCGAAGGGTATTGTATA+CGG + chr2_1:8117723-8117742 Msa0192350:CDS 35.0%
!! TTTCGAAGGGTATTGTATAC+GGG + chr2_1:8117724-8117743 Msa0192350:CDS 35.0%
AAAGAAAACAGAGGTTTGAG+TGG + chr2_1:8118005-8118024 Msa0192350:CDS 35.0%
!!! TTTTGTGTTGCTTCGTTTTC+TGG + chr2_1:8118130-8118149 Msa0192350:three_prime_UTR 35.0%
!! CTTTGTTGTTTGGATTGACA+CGG + chr2_1:8118347-8118366 Msa0192350:three_prime_UTR 35.0%
AAAACGGTAAACTATGCTCA+AGG + chr2_1:8118522-8118541 Msa0192350:three_prime_UTR 35.0%
AATGATGAGGTTCTTATCTC+TGG + chr2_1:8118555-8118574 Msa0192350:three_prime_UTR 35.0%
! TTGTTATTAGTAGAGGTGGA+TGG + chr2_1:8118634-8118653 Msa0192350:three_prime_UTR 35.0%
TATTCTAATGAAGCCAATGG+TGG + chr2_1:8118699-8118718 Msa0192350:three_prime_UTR 35.0%
! ATTTTTGCATTAGCCACCAT+TGG - chr2_1:8118715-8118734 None:intergenic 35.0%
CAACAATTCCAAACTATGCT+AGG - chr2_1:8118740-8118759 None:intergenic 35.0%
!! CTGTTTCTATTCATTCTCTC+TGG + chr2_1:8117206-8117225 Msa0192350:intron 35.0%
! AGTATGTTTCTGAGTTATGG+CGG + chr2_1:8117303-8117322 Msa0192350:CDS 35.0%
AAGAATAGATGATACCTGCT+TGG - chr2_1:8117456-8117475 None:intergenic 35.0%
TAATTAGGGTCTGTTTGGAT+TGG - chr2_1:8117562-8117581 None:intergenic 35.0%
TATACAATACCCTTCGAAAC+AGG - chr2_1:8117723-8117742 None:intergenic 35.0%
!! GTTTCGAAGGGTATTGTATA+CGG + chr2_1:8117723-8117742 Msa0192350:CDS 35.0%
!! TTTCGAAGGGTATTGTATAC+GGG + chr2_1:8117724-8117743 Msa0192350:CDS 35.0%
AAAGAAAACAGAGGTTTGAG+TGG + chr2_1:8118005-8118024 Msa0192350:CDS 35.0%
!!! TTTTGTGTTGCTTCGTTTTC+TGG + chr2_1:8118130-8118149 Msa0192350:three_prime_UTR 35.0%
!! CTTTGTTGTTTGGATTGACA+CGG + chr2_1:8118347-8118366 Msa0192350:three_prime_UTR 35.0%
AAAACGGTAAACTATGCTCA+AGG + chr2_1:8118522-8118541 Msa0192350:three_prime_UTR 35.0%
AATGATGAGGTTCTTATCTC+TGG + chr2_1:8118555-8118574 Msa0192350:three_prime_UTR 35.0%
! TTGTTATTAGTAGAGGTGGA+TGG + chr2_1:8118634-8118653 Msa0192350:three_prime_UTR 35.0%
TATTCTAATGAAGCCAATGG+TGG + chr2_1:8118699-8118718 Msa0192350:three_prime_UTR 35.0%
! ATTTTTGCATTAGCCACCAT+TGG - chr2_1:8118715-8118734 None:intergenic 35.0%
CAACAATTCCAAACTATGCT+AGG - chr2_1:8118740-8118759 None:intergenic 35.0%
GTGAGACAAACCGAGAAAAT+GGG + chr2_1:8117160-8117179 Msa0192350:exon 40.0%
! TGGATGCATACCCATTTTCT+CGG - chr2_1:8117173-8117192 None:intergenic 40.0%
ATATGTTGTGTACCGAGTGA+GGG + chr2_1:8117674-8117693 Msa0192350:CDS 40.0%
GAAATGACCCAAGAATCAAC+TGG + chr2_1:8117928-8117947 Msa0192350:CDS 40.0%
! TAGTGAGCCAGTTGATTCTT+GGG - chr2_1:8117938-8117957 None:intergenic 40.0%
! TTAGTGAGCCAGTTGATTCT+TGG - chr2_1:8117939-8117958 None:intergenic 40.0%
TAATCCTGTCCACAAGCATA+GGG + chr2_1:8117956-8117975 Msa0192350:CDS 40.0%
! TTTCTTTTCCAGCAGAAGTG+AGG - chr2_1:8117992-8118011 None:intergenic 40.0%
TCTGCTGGAAAAGAAAACAG+AGG + chr2_1:8117996-8118015 Msa0192350:CDS 40.0%
TTCTAGGCAAAGACACAGAT+CGG - chr2_1:8118213-8118232 None:intergenic 40.0%
CAAAAACTTGCAGTGTCAGT+GGG - chr2_1:8118378-8118397 None:intergenic 40.0%
! CACTGACACTGCAAGTTTTT+GGG + chr2_1:8118377-8118396 Msa0192350:three_prime_UTR 40.0%
GTCATGTAGCTATCAAGTGA+TGG + chr2_1:8118484-8118503 Msa0192350:three_prime_UTR 40.0%
AGGCACATTGATGAATGATG+AGG + chr2_1:8118542-8118561 Msa0192350:three_prime_UTR 40.0%
GTGAGACAAACCGAGAAAAT+GGG + chr2_1:8117160-8117179 Msa0192350:exon 40.0%
! TGGATGCATACCCATTTTCT+CGG - chr2_1:8117173-8117192 None:intergenic 40.0%
ATATGTTGTGTACCGAGTGA+GGG + chr2_1:8117674-8117693 Msa0192350:CDS 40.0%
GAAATGACCCAAGAATCAAC+TGG + chr2_1:8117928-8117947 Msa0192350:CDS 40.0%
! TAGTGAGCCAGTTGATTCTT+GGG - chr2_1:8117938-8117957 None:intergenic 40.0%
! TTAGTGAGCCAGTTGATTCT+TGG - chr2_1:8117939-8117958 None:intergenic 40.0%
TAATCCTGTCCACAAGCATA+GGG + chr2_1:8117956-8117975 Msa0192350:CDS 40.0%
! TTTCTTTTCCAGCAGAAGTG+AGG - chr2_1:8117992-8118011 None:intergenic 40.0%
TCTGCTGGAAAAGAAAACAG+AGG + chr2_1:8117996-8118015 Msa0192350:CDS 40.0%
TTCTAGGCAAAGACACAGAT+CGG - chr2_1:8118213-8118232 None:intergenic 40.0%
CAAAAACTTGCAGTGTCAGT+GGG - chr2_1:8118378-8118397 None:intergenic 40.0%
! CACTGACACTGCAAGTTTTT+GGG + chr2_1:8118377-8118396 Msa0192350:three_prime_UTR 40.0%
GTCATGTAGCTATCAAGTGA+TGG + chr2_1:8118484-8118503 Msa0192350:three_prime_UTR 40.0%
AGGCACATTGATGAATGATG+AGG + chr2_1:8118542-8118561 Msa0192350:three_prime_UTR 40.0%
CGTGAGACAAACCGAGAAAA+TGG + chr2_1:8117159-8117178 Msa0192350:exon 45.0%
GAAACAGCAGCTGCAATAGA+TGG - chr2_1:8117193-8117212 None:intergenic 45.0%
! GTTATGGCGGAAGAAGCAAT+CGG + chr2_1:8117316-8117335 Msa0192350:CDS 45.0%
GATATGTTGTGTACCGAGTG+AGG + chr2_1:8117673-8117692 Msa0192350:CDS 45.0%
TGAGTAACTCCCTGGTTAGT+GGG - chr2_1:8117753-8117772 None:intergenic 45.0%
TTGAGTAACTCCCTGGTTAG+TGG - chr2_1:8117754-8117773 None:intergenic 45.0%
ACTTCAGTTGAGTAACTCCC+TGG - chr2_1:8117761-8117780 None:intergenic 45.0%
AGTAGGAATTGAGAACCCTG+AGG - chr2_1:8117839-8117858 None:intergenic 45.0%
TCAGGGTTCTCAATTCCTAC+TGG + chr2_1:8117838-8117857 Msa0192350:CDS 45.0%
CAGGGTTCTCAATTCCTACT+GGG + chr2_1:8117839-8117858 Msa0192350:CDS 45.0%
CAATTCCTACTGGGTCAATG+AGG + chr2_1:8117848-8117867 Msa0192350:CDS 45.0%
AGAATCCTCATTGACCCAGT+AGG - chr2_1:8117856-8117875 None:intergenic 45.0%
CTAATCCTGTCCACAAGCAT+AGG + chr2_1:8117955-8117974 Msa0192350:CDS 45.0%
AACAGAGGTTTGAGTGGCAA+AGG + chr2_1:8118011-8118030 Msa0192350:CDS 45.0%
ACAGAGGTTTGAGTGGCAAA+GGG + chr2_1:8118012-8118031 Msa0192350:CDS 45.0%
CCAAAAACTTGCAGTGTCAG+TGG - chr2_1:8118379-8118398 None:intergenic 45.0%
! CCACTGACACTGCAAGTTTT+TGG + chr2_1:8118376-8118395 Msa0192350:three_prime_UTR 45.0%
CAGTAACACCTGCTCTCATT+TGG + chr2_1:8118436-8118455 Msa0192350:three_prime_UTR 45.0%
TTTGACAGCCAAATGAGAGC+AGG - chr2_1:8118447-8118466 None:intergenic 45.0%
AGGTTCTTATCTCTGGTGTC+TGG + chr2_1:8118562-8118581 Msa0192350:three_prime_UTR 45.0%
CGTGAGACAAACCGAGAAAA+TGG + chr2_1:8117159-8117178 Msa0192350:exon 45.0%
GAAACAGCAGCTGCAATAGA+TGG - chr2_1:8117193-8117212 None:intergenic 45.0%
! GTTATGGCGGAAGAAGCAAT+CGG + chr2_1:8117316-8117335 Msa0192350:CDS 45.0%
GATATGTTGTGTACCGAGTG+AGG + chr2_1:8117673-8117692 Msa0192350:CDS 45.0%
TGAGTAACTCCCTGGTTAGT+GGG - chr2_1:8117753-8117772 None:intergenic 45.0%
TTGAGTAACTCCCTGGTTAG+TGG - chr2_1:8117754-8117773 None:intergenic 45.0%
ACTTCAGTTGAGTAACTCCC+TGG - chr2_1:8117761-8117780 None:intergenic 45.0%
AGTAGGAATTGAGAACCCTG+AGG - chr2_1:8117839-8117858 None:intergenic 45.0%
TCAGGGTTCTCAATTCCTAC+TGG + chr2_1:8117838-8117857 Msa0192350:CDS 45.0%
CAGGGTTCTCAATTCCTACT+GGG + chr2_1:8117839-8117858 Msa0192350:CDS 45.0%
CAATTCCTACTGGGTCAATG+AGG + chr2_1:8117848-8117867 Msa0192350:CDS 45.0%
AGAATCCTCATTGACCCAGT+AGG - chr2_1:8117856-8117875 None:intergenic 45.0%
CTAATCCTGTCCACAAGCAT+AGG + chr2_1:8117955-8117974 Msa0192350:CDS 45.0%
AACAGAGGTTTGAGTGGCAA+AGG + chr2_1:8118011-8118030 Msa0192350:CDS 45.0%
ACAGAGGTTTGAGTGGCAAA+GGG + chr2_1:8118012-8118031 Msa0192350:CDS 45.0%
CCAAAAACTTGCAGTGTCAG+TGG - chr2_1:8118379-8118398 None:intergenic 45.0%
! CCACTGACACTGCAAGTTTT+TGG + chr2_1:8118376-8118395 Msa0192350:three_prime_UTR 45.0%
CAGTAACACCTGCTCTCATT+TGG + chr2_1:8118436-8118455 Msa0192350:three_prime_UTR 45.0%
TTTGACAGCCAAATGAGAGC+AGG - chr2_1:8118447-8118466 None:intergenic 45.0%
AGGTTCTTATCTCTGGTGTC+TGG + chr2_1:8118562-8118581 Msa0192350:three_prime_UTR 45.0%
! TTTCTCGGTTTGTCTCACGC+TGG - chr2_1:8117158-8117177 None:intergenic 50.0%
TGAGATTCATGCAGCGTGTG+AGG + chr2_1:8117345-8117364 Msa0192350:CDS 50.0%
!! GCTTGGCCTTGTAACCCAAA+CGG - chr2_1:8117439-8117458 None:intergenic 50.0%
! TTTGGGTTACAAGGCCAAGC+AGG + chr2_1:8117439-8117458 Msa0192350:CDS 50.0%
TACGTAGAGGTGGTAGGAAG+AGG + chr2_1:8117697-8117716 Msa0192350:CDS 50.0%
TAGGAAGAGGCCTGTTTCGA+AGG + chr2_1:8117710-8117729 Msa0192350:CDS 50.0%
AGGAAGAGGCCTGTTTCGAA+GGG + chr2_1:8117711-8117730 Msa0192350:CDS 50.0%
AGTTCCCTATGCTTGTGGAC+AGG - chr2_1:8117963-8117982 None:intergenic 50.0%
CACGAAGTTCCCTATGCTTG+TGG - chr2_1:8117968-8117987 None:intergenic 50.0%
CACAAGCATAGGGAACTTCG+TGG + chr2_1:8117966-8117985 Msa0192350:CDS 50.0%
TTCTCTTCCAGTTTGCCCTG+CGG - chr2_1:8118064-8118083 None:intergenic 50.0%
ACGATAACGACGGAGAGACA+AGG - chr2_1:8118093-8118112 None:intergenic 50.0%
! TTTCTCGGTTTGTCTCACGC+TGG - chr2_1:8117158-8117177 None:intergenic 50.0%
TGAGATTCATGCAGCGTGTG+AGG + chr2_1:8117345-8117364 Msa0192350:CDS 50.0%
!! GCTTGGCCTTGTAACCCAAA+CGG - chr2_1:8117439-8117458 None:intergenic 50.0%
! TTTGGGTTACAAGGCCAAGC+AGG + chr2_1:8117439-8117458 Msa0192350:CDS 50.0%
TACGTAGAGGTGGTAGGAAG+AGG + chr2_1:8117697-8117716 Msa0192350:CDS 50.0%
TAGGAAGAGGCCTGTTTCGA+AGG + chr2_1:8117710-8117729 Msa0192350:CDS 50.0%
AGGAAGAGGCCTGTTTCGAA+GGG + chr2_1:8117711-8117730 Msa0192350:CDS 50.0%
AGTTCCCTATGCTTGTGGAC+AGG - chr2_1:8117963-8117982 None:intergenic 50.0%
CACGAAGTTCCCTATGCTTG+TGG - chr2_1:8117968-8117987 None:intergenic 50.0%
CACAAGCATAGGGAACTTCG+TGG + chr2_1:8117966-8117985 Msa0192350:CDS 50.0%
TTCTCTTCCAGTTTGCCCTG+CGG - chr2_1:8118064-8118083 None:intergenic 50.0%
ACGATAACGACGGAGAGACA+AGG - chr2_1:8118093-8118112 None:intergenic 50.0%
TCATGCAGCGTGTGAGGTGT+TGG + chr2_1:8117351-8117370 Msa0192350:CDS 55.0%
! CATGCAGCGTGTGAGGTGTT+GGG + chr2_1:8117352-8117371 Msa0192350:CDS 55.0%
GGGTCTGGTGAGACGAACAA+TGG - chr2_1:8117396-8117415 None:intergenic 55.0%
TTTGTCAGGACGAGTGGGTC+TGG - chr2_1:8117411-8117430 None:intergenic 55.0%
! CGAGCTTTGTCAGGACGAGT+GGG - chr2_1:8117416-8117435 None:intergenic 55.0%
TCCTGACAAAGCTCGCCGTT+TGG + chr2_1:8117421-8117440 Msa0192350:CDS 55.0%
CCTGACAAAGCTCGCCGTTT+GGG + chr2_1:8117422-8117441 Msa0192350:CDS 55.0%
! AGCTCGCCGTTTGGGTTACA+AGG + chr2_1:8117430-8117449 Msa0192350:CDS 55.0%
! TACCGAGTGAGGGTACGTAG+AGG + chr2_1:8117684-8117703 Msa0192350:CDS 55.0%
CACCTCTACGTACCCTCACT+CGG - chr2_1:8117689-8117708 None:intergenic 55.0%
! TGAGGGTACGTAGAGGTGGT+AGG + chr2_1:8117691-8117710 Msa0192350:CDS 55.0%
ATACGGGAAGCCCACTAACC+AGG + chr2_1:8117740-8117759 Msa0192350:CDS 55.0%
TACGGGAAGCCCACTAACCA+GGG + chr2_1:8117741-8117760 Msa0192350:CDS 55.0%
GAGCAAGAGATCAGTTGCCG+AGG + chr2_1:8117788-8117807 Msa0192350:CDS 55.0%
TCTCTTCCAGTTTGCCCTGC+GGG - chr2_1:8118063-8118082 None:intergenic 55.0%
TCATGCAGCGTGTGAGGTGT+TGG + chr2_1:8117351-8117370 Msa0192350:CDS 55.0%
! CATGCAGCGTGTGAGGTGTT+GGG + chr2_1:8117352-8117371 Msa0192350:CDS 55.0%
GGGTCTGGTGAGACGAACAA+TGG - chr2_1:8117396-8117415 None:intergenic 55.0%
TTTGTCAGGACGAGTGGGTC+TGG - chr2_1:8117411-8117430 None:intergenic 55.0%
! CGAGCTTTGTCAGGACGAGT+GGG - chr2_1:8117416-8117435 None:intergenic 55.0%
TCCTGACAAAGCTCGCCGTT+TGG + chr2_1:8117421-8117440 Msa0192350:CDS 55.0%
CCTGACAAAGCTCGCCGTTT+GGG + chr2_1:8117422-8117441 Msa0192350:CDS 55.0%
! AGCTCGCCGTTTGGGTTACA+AGG + chr2_1:8117430-8117449 Msa0192350:CDS 55.0%
! TACCGAGTGAGGGTACGTAG+AGG + chr2_1:8117684-8117703 Msa0192350:CDS 55.0%
CACCTCTACGTACCCTCACT+CGG - chr2_1:8117689-8117708 None:intergenic 55.0%
! TGAGGGTACGTAGAGGTGGT+AGG + chr2_1:8117691-8117710 Msa0192350:CDS 55.0%
ATACGGGAAGCCCACTAACC+AGG + chr2_1:8117740-8117759 Msa0192350:CDS 55.0%
TACGGGAAGCCCACTAACCA+GGG + chr2_1:8117741-8117760 Msa0192350:CDS 55.0%
GAGCAAGAGATCAGTTGCCG+AGG + chr2_1:8117788-8117807 Msa0192350:CDS 55.0%
TCTCTTCCAGTTTGCCCTGC+GGG - chr2_1:8118063-8118082 None:intergenic 55.0%
! TTTGTCTCACGCTGGCTGGC+AGG - chr2_1:8117150-8117169 None:intergenic 60.0%
! TCGGTTTGTCTCACGCTGGC+TGG - chr2_1:8117154-8117173 None:intergenic 60.0%
! GCGAGCTTTGTCAGGACGAG+TGG - chr2_1:8117417-8117436 None:intergenic 60.0%
! CCCAAACGGCGAGCTTTGTC+AGG - chr2_1:8117425-8117444 None:intergenic 60.0%
! CGAGTGAGGGTACGTAGAGG+TGG + chr2_1:8117687-8117706 Msa0192350:CDS 60.0%
CTTCGTGGCCTCACTTCTGC+TGG + chr2_1:8117981-8118000 Msa0192350:CDS 60.0%
AGTTTGCCCTGCGGGAAGGA+CGG - chr2_1:8118055-8118074 None:intergenic 60.0%
TTCCAGTTTGCCCTGCGGGA+AGG - chr2_1:8118059-8118078 None:intergenic 60.0%
! TTTGTCTCACGCTGGCTGGC+AGG - chr2_1:8117150-8117169 None:intergenic 60.0%
! TCGGTTTGTCTCACGCTGGC+TGG - chr2_1:8117154-8117173 None:intergenic 60.0%
! GCGAGCTTTGTCAGGACGAG+TGG - chr2_1:8117417-8117436 None:intergenic 60.0%
! CCCAAACGGCGAGCTTTGTC+AGG - chr2_1:8117425-8117444 None:intergenic 60.0%
! CGAGTGAGGGTACGTAGAGG+TGG + chr2_1:8117687-8117706 Msa0192350:CDS 60.0%
CTTCGTGGCCTCACTTCTGC+TGG + chr2_1:8117981-8118000 Msa0192350:CDS 60.0%
AGTTTGCCCTGCGGGAAGGA+CGG - chr2_1:8118055-8118074 None:intergenic 60.0%
TTCCAGTTTGCCCTGCGGGA+AGG - chr2_1:8118059-8118078 None:intergenic 60.0%
! TCAGTTGCCGAGGAGCGTGC+TGG + chr2_1:8117798-8117817 Msa0192350:CDS 65.0%
! CAGTTGCCGAGGAGCGTGCT+GGG + chr2_1:8117799-8117818 Msa0192350:CDS 65.0%
CTTCCTCCCAGCACGCTCCT+CGG - chr2_1:8117808-8117827 None:intergenic 65.0%
GAGCGTGCTGGGAGGAAGCT+CGG + chr2_1:8117810-8117829 Msa0192350:CDS 65.0%
! GAGGAAGCTCGGTGGCCTCA+GGG + chr2_1:8117821-8117840 Msa0192350:CDS 65.0%
! TGCGGGAAGGACGGGCTTTG+TGG - chr2_1:8118046-8118065 None:intergenic 65.0%
ACAAAGCCCGTCCTTCCCGC+AGG + chr2_1:8118045-8118064 Msa0192350:CDS 65.0%
CAAAGCCCGTCCTTCCCGCA+GGG + chr2_1:8118046-8118065 Msa0192350:CDS 65.0%
GTTTGCCCTGCGGGAAGGAC+GGG - chr2_1:8118054-8118073 None:intergenic 65.0%
GTCCTTCCCGCAGGGCAAAC+TGG + chr2_1:8118054-8118073 Msa0192350:CDS 65.0%
! TCAGTTGCCGAGGAGCGTGC+TGG + chr2_1:8117798-8117817 Msa0192350:CDS 65.0%
! CAGTTGCCGAGGAGCGTGCT+GGG + chr2_1:8117799-8117818 Msa0192350:CDS 65.0%
CTTCCTCCCAGCACGCTCCT+CGG - chr2_1:8117808-8117827 None:intergenic 65.0%
GAGCGTGCTGGGAGGAAGCT+CGG + chr2_1:8117810-8117829 Msa0192350:CDS 65.0%
! GAGGAAGCTCGGTGGCCTCA+GGG + chr2_1:8117821-8117840 Msa0192350:CDS 65.0%
! TGCGGGAAGGACGGGCTTTG+TGG - chr2_1:8118046-8118065 None:intergenic 65.0%
ACAAAGCCCGTCCTTCCCGC+AGG + chr2_1:8118045-8118064 Msa0192350:CDS 65.0%
CAAAGCCCGTCCTTCCCGCA+GGG + chr2_1:8118046-8118065 Msa0192350:CDS 65.0%
GTTTGCCCTGCGGGAAGGAC+GGG - chr2_1:8118054-8118073 None:intergenic 65.0%
GTCCTTCCCGCAGGGCAAAC+TGG + chr2_1:8118054-8118073 Msa0192350:CDS 65.0%
! TTGCCGAGGAGCGTGCTGGG+AGG + chr2_1:8117802-8117821 Msa0192350:CDS 70.0%
CGTGCTGGGAGGAAGCTCGG+TGG + chr2_1:8117813-8117832 Msa0192350:CDS 70.0%
! GGAGGAAGCTCGGTGGCCTC+AGG + chr2_1:8117820-8117839 Msa0192350:CDS 70.0%
! TTGCCGAGGAGCGTGCTGGG+AGG + chr2_1:8117802-8117821 Msa0192350:CDS 70.0%
CGTGCTGGGAGGAAGCTCGG+TGG + chr2_1:8117813-8117832 Msa0192350:CDS 70.0%
! GGAGGAAGCTCGGTGGCCTC+AGG + chr2_1:8117820-8117839 Msa0192350:CDS 70.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 8117139 8118823 8117139 ID=Msa0192350;Name=Msa0192350
chr2_1 mRNA 8117139 8118823 8117139 ID=Msa0192350-mRNA-1;Parent=Msa0192350;Name=Msa0192350-mRNA-1;_AED=0.06;_eAED=0.06;_QI=39|1|1|1|1|1|3|708|204
chr2_1 exon 8117139 8117181 8117139 ID=Msa0192350-mRNA-1:exon:3754;Parent=Msa0192350-mRNA-1
chr2_1 exon 8117294 8117460 8117294 ID=Msa0192350-mRNA-1:exon:3755;Parent=Msa0192350-mRNA-1
chr2_1 exon 8117672 8118823 8117672 ID=Msa0192350-mRNA-1:exon:3756;Parent=Msa0192350-mRNA-1
chr2_1 five_prime_UTR 8117139 8117177 8117139 ID=Msa0192350-mRNA-1:five_prime_utr;Parent=Msa0192350-mRNA-1
chr2_1 CDS 8117178 8117181 8117178 ID=Msa0192350-mRNA-1:cds;Parent=Msa0192350-mRNA-1
chr2_1 CDS 8117294 8117460 8117294 ID=Msa0192350-mRNA-1:cds;Parent=Msa0192350-mRNA-1
chr2_1 CDS 8117672 8118115 8117672 ID=Msa0192350-mRNA-1:cds;Parent=Msa0192350-mRNA-1
chr2_1 three_prime_UTR 8118116 8118823 8118116 ID=Msa0192350-mRNA-1:three_prime_utr;Parent=Msa0192350-mRNA-1
Gene Sequence

>Msa0192350

ATGGGTGCATACAAGTATGTTTCTGAGTTATGGCGGAAGAAGCAATCGGATGTGATGAGATTCATGCAGCGTGTGAGGTGTTGGGAGTATCGTCAACAATCTTCCATTGTTCGTCTCACCAGACCCACTCGTCCTGACAAAGCTCGCCGTTTGGGTTACAAGGCCAAGCAGGGATATGTTGTGTACCGAGTGAGGGTACGTAGAGGTGGTAGGAAGAGGCCTGTTTCGAAGGGTATTGTATACGGGAAGCCCACTAACCAGGGAGTTACTCAACTGAAGTTTCAAAGGAGCAAGAGATCAGTTGCCGAGGAGCGTGCTGGGAGGAAGCTCGGTGGCCTCAGGGTTCTCAATTCCTACTGGGTCAATGAGGATTCTACCTTCAAGTATTTTGAGGTCATTTTGGTTGATGTTGCTCATAGTGCTATAAGAAATGACCCAAGAATCAACTGGCTCACTAATCCTGTCCACAAGCATAGGGAACTTCGTGGCCTCACTTCTGCTGGAAAAGAAAACAGAGGTTTGAGTGGCAAAGGGCATCGTTACCACAAAGCCCGTCCTTCCCGCAGGGCAAACTGGAAGAGAAACAACACCTTGTCTCTCCGTCGTTATCGTTGA
Protein sequence

>Msa0192350

MGAYKYVSELWRKKQSDVMRFMQRVRCWEYRQQSSIVRLTRPTRPDKARRLGYKAKQGYVVYRVRVRRGGRKRPVSKGIVYGKPTNQGVTQLKFQRSKRSVAEERAGRKLGGLRVLNSYWVNEDSTFKYFEVILVDVAHSAIRNDPRINWLTNPVHKHRELRGLTSAGKENRGLSGKGHRYHKARPSRRANWKRNNTLSLRRYR*