AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0200190

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0200190 MtrunA17_Chr2g0294911 85.156 256 12 3 1 230 1 256 9.05e-138 400
Msa0200190 MtrunA17_Chr4g0067981 68.726 259 52 4 1 230 2 260 6.84e-106 320
Msa0200190 MtrunA17_Chr1g0161431 61.463 205 69 3 36 230 41 245 9.77e-79 248
Msa0200190 MtrunA17_Chr3g0137101 53.425 219 91 2 23 230 25 243 1.20e-71 230
Msa0200190 MtrunA17_Chr3g0123131 54.950 202 84 2 36 230 43 244 2.62e-70 225
Msa0200190 MtrunA17_Chr8g0376881 53.140 207 78 3 36 230 55 254 5.48e-64 208
Msa0200190 MtrunA17_Chr2g0304841 50.446 224 88 3 30 230 35 258 1.44e-63 210
Msa0200190 MtrunA17_Chr1g0149841 51.232 203 90 2 36 230 35 236 1.73e-63 206
Msa0200190 MtrunA17_Chr4g0074421 48.768 203 82 4 56 247 2 193 1.98e-60 189
Msa0200190 MtrunA17_Chr7g0275891 49.074 216 88 4 36 230 35 249 8.02e-60 192
Msa0200190 MtrunA17_Chr4g0074401 51.485 202 73 3 45 230 56 248 4.61e-59 196
Msa0200190 MtrunA17_Chr4g0074411 51.485 202 88 3 36 230 48 246 5.83e-59 196
Msa0200190 MtrunA17_Chr7g0275401 49.261 203 95 2 36 230 35 237 7.83e-58 185
Msa0200190 MtrunA17_Chr1g0149851 48.624 218 88 3 36 230 35 251 2.85e-55 185
Msa0200190 MtrunA17_Chr1g0149831 51.412 177 68 4 65 230 1 170 2.61e-49 161
Msa0200190 MtrunA17_Chr3g0134461 44.898 196 62 2 36 230 38 188 1.54e-46 153
Msa0200190 MtrunA17_Chr3g0134481 40.513 195 61 2 36 230 38 177 1.09e-43 146
Msa0200190 MtrunA17_Chr4g0009851 36.410 195 103 3 36 230 41 214 1.77e-40 141
Msa0200190 MtrunA17_Chr4g0009901 39.234 209 105 5 22 230 27 213 3.80e-40 139
Msa0200190 MtrunA17_Chr4g0009981 40.000 180 88 3 51 230 2 161 5.48e-39 136
Msa0200190 MtrunA17_Chr6g0471041 39.000 200 91 5 36 230 44 217 2.44e-37 135
Msa0200190 MtrunA17_Chr4g0011071 33.333 159 86 2 53 211 36 174 4.56e-28 105
Msa0200190 MtrunA17_Chr8g0391911 33.796 216 106 5 36 230 39 238 2.03e-27 105
Msa0200190 MtrunA17_Chr6g0473731 37.415 147 74 3 86 230 10 140 1.74e-23 98.2
Msa0200190 MtrunA17_Chr8g0383961 43.750 96 54 0 36 131 44 139 1.81e-22 92.8
Msa0200190 MtrunA17_Chr4g0009991 38.346 133 61 2 98 230 1 112 4.16e-21 85.5
Msa0200190 MtrunA17_Chr3g0127941 35.333 150 82 5 26 161 65 213 1.47e-17 81.6
Msa0200190 MtrunA17_Chr8g0345901 41.071 112 52 4 29 128 48 157 5.45e-17 80.1
Msa0200190 MtrunA17_Chr3g0091641 36.522 115 64 2 25 130 33 147 1.10e-15 75.9
Msa0200190 MtrunA17_Chr4g0030271 37.719 114 59 3 26 128 98 210 1.41e-15 75.9
Msa0200190 MtrunA17_Chr7g0223971 27.841 176 85 2 88 230 1 167 1.43e-15 75.5
Msa0200190 MtrunA17_Chr1g0181811 36.283 113 62 2 26 128 94 206 2.60e-15 75.1
Msa0200190 MtrunA17_Chr6g0456631 35.789 95 58 2 36 128 39 132 2.65e-15 71.6
Msa0200190 MtrunA17_Chr7g0276361 38.710 124 60 4 26 136 23 143 2.91e-15 74.7
Msa0200190 MtrunA17_Chr4g0061021 37.755 98 58 1 36 130 59 156 5.42e-15 73.9
Msa0200190 MtrunA17_Chr4g0031961 48.718 78 32 2 161 230 99 176 5.20e-14 70.1
Msa0200190 MtrunA17_Chr7g0255711 52.830 53 25 0 178 230 84 136 4.49e-12 64.7
Msa0200190 MtrunA17_Chr5g0400241 41.573 89 46 3 45 131 53 137 7.58e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0200190 AT3G16857.1 63.519 233 60 6 23 230 50 282 1.42e-93 288
Msa0200190 AT3G16857.2 63.519 233 60 6 23 230 50 282 2.93e-93 288
Msa0200190 AT3G16857.3 66.038 212 47 6 44 230 1 212 4.90e-88 273
Msa0200190 AT2G25180.1 57.711 201 76 3 36 230 43 240 8.12e-67 217
Msa0200190 AT2G01760.2 49.784 231 96 4 20 230 43 273 1.14e-64 207
Msa0200190 AT2G01760.1 52.632 209 85 3 36 230 37 245 2.57e-64 205
Msa0200190 AT4G31920.1 52.804 214 73 3 30 230 30 228 3.96e-64 209
Msa0200190 AT1G67710.1 54.634 205 81 5 36 230 37 239 1.32e-60 199
Msa0200190 AT5G58080.1 49.528 212 74 3 36 230 44 239 8.70e-59 197
Msa0200190 AT2G01760.3 51.111 180 74 3 65 230 1 180 3.13e-50 167
Msa0200190 AT1G67710.2 53.977 176 69 5 65 230 1 174 1.40e-48 166
Msa0200190 AT3G62670.1 35.470 234 136 5 3 230 37 261 1.23e-38 139
Msa0200190 AT5G49240.1 38.967 213 100 5 36 230 68 268 7.26e-38 134
Msa0200190 AT1G49190.1 35.885 209 104 6 42 230 66 264 3.81e-34 127
Msa0200190 AT1G67710.3 48.077 156 69 5 85 230 12 165 3.53e-32 122
Msa0200190 AT5G07210.1 31.364 220 114 7 45 230 59 275 1.63e-24 102
Msa0200190 AT5G07210.2 31.364 220 114 7 45 230 51 267 1.68e-24 102
Msa0200190 AT1G49190.2 50.000 88 44 0 42 129 66 153 6.79e-24 100
Msa0200190 AT2G27070.1 29.832 238 127 8 27 230 39 270 7.15e-24 100
Msa0200190 AT2G46790.3 40.541 111 54 3 29 128 49 158 1.59e-18 84.3
Msa0200190 AT2G46790.1 40.541 111 54 3 29 128 49 158 1.71e-18 84.3
Msa0200190 AT5G24470.1 37.391 115 60 3 26 129 59 172 9.70e-17 79.7
Msa0200190 AT5G60100.7 38.053 113 60 2 26 128 73 185 2.98e-15 74.7
Msa0200190 AT5G60100.6 38.053 113 60 2 26 128 73 185 2.98e-15 74.7
Msa0200190 AT5G60100.4 38.053 113 60 2 26 128 73 185 2.98e-15 74.7
Msa0200190 AT5G60100.5 38.053 113 60 2 26 128 73 185 3.89e-15 74.7
Msa0200190 AT5G60100.3 38.053 113 60 2 26 128 73 185 3.89e-15 74.7
Msa0200190 AT5G60100.1 38.053 113 60 2 26 128 73 185 3.89e-15 74.7
Msa0200190 AT5G59570.2 53.226 62 29 0 169 230 125 186 3.98e-15 73.6
Msa0200190 AT5G59570.1 53.226 62 29 0 169 230 125 186 3.98e-15 73.6
Msa0200190 AT5G60100.2 38.053 113 60 2 26 128 73 185 4.53e-15 74.7
Msa0200190 AT5G61380.1 38.679 106 55 3 35 130 37 142 8.38e-14 70.9
Msa0200190 AT3G46640.2 51.613 62 30 0 169 230 128 189 8.54e-14 70.1
Msa0200190 AT3G46640.1 51.613 62 30 0 169 230 128 189 8.54e-14 70.1
Msa0200190 AT3G46640.3 51.613 62 30 0 169 230 128 189 9.38e-14 70.1
Msa0200190 AT5G02810.1 38.053 113 60 2 26 128 87 199 1.12e-13 70.5
Msa0200190 AT2G40970.1 52.830 53 25 0 178 230 98 150 1.37e-12 65.9
Msa0200190 AT5G44190.2 56.604 53 23 0 178 230 140 192 2.03e-12 66.2
Msa0200190 AT5G44190.1 56.604 53 23 0 178 230 140 192 2.31e-12 66.2
Msa0200190 AT5G05090.1 52.830 53 25 0 178 230 74 126 2.88e-12 65.1
Msa0200190 AT3G10760.1 52.830 53 25 0 178 230 98 150 5.22e-12 65.1
Msa0200190 AT4G18020.3 41.573 89 49 2 45 132 59 145 3.50e-11 63.2
Msa0200190 AT4G18020.2 41.573 89 49 2 45 132 59 145 3.50e-11 63.2
Msa0200190 AT4G18020.1 41.573 89 49 2 45 132 59 145 3.50e-11 63.2
Msa0200190 AT4G18020.6 41.573 89 49 2 45 132 59 145 3.50e-11 63.2
Msa0200190 AT4G18020.9 41.573 89 49 2 45 132 59 145 3.50e-11 63.2
Msa0200190 AT4G18020.8 41.573 89 49 2 45 132 59 145 3.68e-11 63.2
Msa0200190 AT4G18020.11 41.573 89 49 2 45 132 59 145 3.68e-11 63.2
Msa0200190 AT4G18020.4 41.573 89 49 2 45 132 59 145 3.68e-11 63.2
Msa0200190 AT4G18020.5 41.573 89 49 2 45 132 59 145 3.68e-11 63.2
Msa0200190 AT4G18020.10 41.573 89 49 2 45 132 59 145 3.68e-11 63.2
Msa0200190 AT4G18020.7 41.573 89 49 2 45 132 59 145 3.68e-11 63.2

Find 129 sgRNAs with CRISPR-Local

Find 336 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAGCTTCATCAACAGTTTA+TGG 0.134096 2_1:+19782907 Msa0200190:CDS
CTTCAAACCCTTTCAGCTTC+TGG 0.197138 2_1:+19784649 Msa0200190:three_prime_UTR
GTTGTTGTTGTTGAGTTTCA+TGG 0.237423 2_1:-19781790 None:intergenic
GAAGCTACTTCGGGAATTAA+AGG 0.286940 2_1:+19785102 Msa0200190:three_prime_UTR
TATGGATGGATTCAAACTTT+TGG 0.295920 2_1:+19782416 Msa0200190:CDS
GATAATGAGTTTGACTTAGA+TGG 0.303961 2_1:+19785705 Msa0200190:three_prime_UTR
AGTACGCCAGAGTCAATATT+TGG 0.308653 2_1:+19784601 Msa0200190:three_prime_UTR
CACCAGTCTGCTCAATCCTT+TGG 0.321223 2_1:+19784844 Msa0200190:three_prime_UTR
TTGAAGATCAATTTCATTTA+CGG 0.326626 2_1:-19784632 None:intergenic
GAGAACTCAAAACTAAGATT+TGG 0.328580 2_1:+19784727 Msa0200190:three_prime_UTR
TTCAGCTTCTGGCCAGTTCT+CGG 0.333161 2_1:+19784660 Msa0200190:three_prime_UTR
ACTTCGGGAATTAAAGGATT+AGG 0.335057 2_1:+19785108 Msa0200190:three_prime_UTR
AAGAAGATGAAGCAGATGAT+AGG 0.337750 2_1:+19782840 Msa0200190:CDS
TGCTTTGGCTCAATGTTGAT+AGG 0.342601 2_1:-19784808 None:intergenic
ACCTTATCTCCGACCAGTTC+CGG 0.349927 2_1:+19781856 Msa0200190:CDS
TTGAGCAGACTGGTGAAAAT+TGG 0.352834 2_1:-19784836 None:intergenic
TGAAAATTGGCAAACTGCTT+TGG 0.368522 2_1:-19784823 None:intergenic
CGACGTGCATATGCCTGATA+TGG 0.368808 2_1:+19782398 Msa0200190:CDS
TCGAGGCTTTGAAGAATATA+TGG 0.369544 2_1:+19782666 Msa0200190:CDS
CTTCGGGAATTAAAGGATTA+GGG 0.371380 2_1:+19785109 Msa0200190:three_prime_UTR
AAACAATGCTTACTAATAAC+AGG 0.383212 2_1:-19782465 None:intergenic
CTATTGACTGATATTCCATT+TGG 0.387671 2_1:-19785018 None:intergenic
TTCGGAAGAGAAAGAATAAC+TGG 0.397525 2_1:+19782699 Msa0200190:CDS
TCATTTAGCATCTGACCTCT+TGG 0.399993 2_1:-19784940 None:intergenic
CTAGACTTTGCACCGAGAAC+TGG 0.401730 2_1:-19784672 None:intergenic
TAACAGGATCTGTTATAAAC+TGG 0.403457 2_1:-19785747 None:intergenic
AACCTCAGCTCTCTTGCATT+TGG 0.403620 2_1:-19782325 None:intergenic
CCATCAAAAGTCCAATGATT+GGG 0.406485 2_1:+19785161 Msa0200190:three_prime_UTR
ATTTGCTAATCCCCAATCAT+TGG 0.406561 2_1:-19785172 None:intergenic
CAAAGAAGCCTCGAGTTGTT+TGG 0.412556 2_1:+19782879 Msa0200190:CDS
TGAGGTGTTGACCAACATTA+TGG 0.417819 2_1:-19785338 None:intergenic
TATTCAAGTTACTTTGCTGC+TGG 0.418842 2_1:-19784567 None:intergenic
ATGGAAATGATGAATGTTCC+TGG 0.426157 2_1:+19783246 Msa0200190:CDS
AGTTTGAATCCATCCATATC+AGG 0.428415 2_1:-19782411 None:intergenic
TCCATCAAAAGTCCAATGAT+TGG 0.430749 2_1:+19785160 Msa0200190:three_prime_UTR
ACACGGAGACCCGCCGGAAC+TGG 0.440539 2_1:-19781869 None:intergenic
TCCAATGAATTGTCGACAAA+AGG 0.449241 2_1:-19785365 None:intergenic
ATAAGGTTGCCGGAGTTTGA+TGG 0.460200 2_1:-19781840 None:intergenic
TATGGCTGTTGTGAATCAAT+TGG 0.460490 2_1:+19782925 Msa0200190:CDS
TGATTAGAAAGAGAGCTAAC+AGG 0.464856 2_1:-19785763 None:intergenic
TGGAAAGATCTGAAGCAATC+TGG 0.465336 2_1:+19782719 Msa0200190:CDS
TTCTCGCAGCACAAAGGCGT+TGG 0.468445 2_1:+19785675 Msa0200190:intron
TTGTCGAGTCTGTTGGATAG+AGG 0.472904 2_1:-19785286 None:intergenic
ATGGCTGTTGTGAATCAATT+GGG 0.473696 2_1:+19782926 Msa0200190:CDS
ATGCAACCTACAGGACCAAA+TGG 0.473881 2_1:+19785003 Msa0200190:three_prime_UTR
ACATATGAGCAGCAGTAAAC+CGG 0.474063 2_1:+19784765 Msa0200190:three_prime_UTR
CTAGCCAAGCTTCAAGCAGC+AGG 0.477941 2_1:+19784691 Msa0200190:three_prime_UTR
GGAACATATTGGATTGGAGA+TGG 0.480393 2_1:+19782437 Msa0200190:CDS
AGAAGATGAAGCAGATGATA+GGG 0.481453 2_1:+19782841 Msa0200190:CDS
AGATCAATGTTGCCTTGTAA+AGG 0.489412 2_1:-19785231 None:intergenic
TTCCCTCTTAGATGCACTCC+GGG 0.489466 2_1:+19785042 Msa0200190:three_prime_UTR
GTAGGTTGCATTAAAGATGA+TGG 0.494028 2_1:-19784991 None:intergenic
CAACATGCAAATCCTTTACA+AGG 0.495519 2_1:+19785219 Msa0200190:three_prime_UTR
TACTCATCTTCAACGAATGA+AGG 0.499328 2_1:+19782791 Msa0200190:CDS
GATATTCCATTTGGTCCTGT+AGG 0.499380 2_1:-19785009 None:intergenic
GCTTTGGCTCAATGTTGATA+GGG 0.500235 2_1:-19784807 None:intergenic
CAAAACTAAGATTTGGAGAT+GGG 0.507173 2_1:+19784734 Msa0200190:three_prime_UTR
GCCGGAACTGGTCGGAGATA+AGG 0.511945 2_1:-19781857 None:intergenic
ATTCAAGTTACTTTGCTGCT+GGG 0.515195 2_1:-19784566 None:intergenic
GTTGAGAAGCATCATATGTC+AGG 0.515544 2_1:-19785200 None:intergenic
CATGAGCGTATTTCTGAACC+AGG 0.515894 2_1:+19785561 Msa0200190:three_prime_UTR
GATTGGGGATTAGCAAATAC+AGG 0.520281 2_1:+19785177 Msa0200190:three_prime_UTR
TTCAAAGCCTCGATTCGCAC+AGG 0.523205 2_1:-19782656 None:intergenic
ATATATGGCAGCATGTGGTT+CGG 0.524503 2_1:+19782681 Msa0200190:CDS
CTGGTCGGAGATAAGGTTGC+CGG 0.525749 2_1:-19781850 None:intergenic
ATATCCAGTAGCAGTACAAA+AGG 0.527621 2_1:+19785066 Msa0200190:three_prime_UTR
TGACTGACTTGATCAGAGAA+GGG 0.534175 2_1:-19785529 None:intergenic
AAACCGGTGAACTTTCTTCA+CGG 0.535622 2_1:+19784781 Msa0200190:three_prime_UTR
ATACAAATTCTCATGCTACA+AGG 0.536556 2_1:+19784965 Msa0200190:three_prime_UTR
TCAAAACTAAGATTTGGAGA+TGG 0.536886 2_1:+19784733 Msa0200190:three_prime_UTR
TTGACTGACTTGATCAGAGA+AGG 0.537467 2_1:-19785530 None:intergenic
TTGTATCTTCGAAGGTTGAG+TGG 0.537963 2_1:+19784538 Msa0200190:CDS
GAACTGGCCAGAAGCTGAAA+GGG 0.541735 2_1:-19784656 None:intergenic
TCGCATATTTGCGCTGCCAA+AGG 0.548078 2_1:-19784860 None:intergenic
AATACCGTTTGTATCTTCGA+AGG 0.548651 2_1:+19784530 Msa0200190:CDS
AGAACTGGCCAGAAGCTGAA+AGG 0.554761 2_1:-19784657 None:intergenic
TCATCTTTAATGCAACCTAC+AGG 0.556863 2_1:+19784994 Msa0200190:three_prime_UTR
TTAGTGATGTCGGCTGATGA+TGG 0.559693 2_1:+19782578 Msa0200190:intron
ATACCCGGAGTGCATCTAAG+AGG 0.561462 2_1:-19785045 None:intergenic
AAGCCCTTGGTTGATGCAGA+TGG 0.564484 2_1:+19784906 Msa0200190:three_prime_UTR
TAGTTGCCAATGTAAATAGT+TGG 0.567960 2_1:+19785786 Msa0200190:three_prime_UTR
GGAACACCAACTATTTACAT+TGG 0.570285 2_1:-19785792 None:intergenic
CTCTGATCAAGTCAGTCAAG+AGG 0.573459 2_1:+19785534 Msa0200190:three_prime_UTR
TTGATCTCAATGTTTGTACT+CGG 0.576687 2_1:-19785505 None:intergenic
ATTCCGTGAAGAAAGTTCAC+CGG 0.576781 2_1:-19784784 None:intergenic
GTTCCTCCAAATATTGACTC+TGG 0.578483 2_1:-19784607 None:intergenic
TGCCAAAGGATTGAGCAGAC+TGG 0.579512 2_1:-19784846 None:intergenic
TTTCCCTCTTAGATGCACTC+CGG 0.583189 2_1:+19785041 Msa0200190:three_prime_UTR
TGACTCTGGCGTACTCATGA+AGG 0.583909 2_1:-19784593 None:intergenic
TTGTCGACAAAAGGTGTATG+AGG 0.584821 2_1:-19785356 None:intergenic
GCAAGAGCTCAAAGAAGAGG+AGG 0.587165 2_1:+19782813 Msa0200190:CDS
GACTGACTTGATCAGAGAAG+GGG 0.587939 2_1:-19785528 None:intergenic
TATCTCCGACCAGTTCCGGC+GGG 0.588949 2_1:+19781860 Msa0200190:CDS
CAAGGTGATCACCATAATGT+TGG 0.591086 2_1:+19785327 Msa0200190:three_prime_UTR
AAGTCCTGCTGCTTGAAGCT+TGG 0.592461 2_1:-19784695 None:intergenic
AGATGAGTAATTAGCATCAG+TGG 0.594629 2_1:-19782777 None:intergenic
CTGCTGCAAAGAGAAGCCCT+TGG 0.595593 2_1:+19784893 Msa0200190:three_prime_UTR
ACAAGTACACGGAGACCCGC+CGG 0.596595 2_1:-19781875 None:intergenic
TGGTGAAGTTGAGGTTGACA+TGG 0.599570 2_1:-19781820 None:intergenic
TTATCTCCGACCAGTTCCGG+CGG 0.605100 2_1:+19781859 Msa0200190:CDS
AAGATCAATTTCATTTACGG+AGG 0.607064 2_1:-19784629 None:intergenic
GAGCCATCTGCATCAACCAA+GGG 0.609724 2_1:-19784909 None:intergenic
TGAGCCATCTGCATCAACCA+AGG 0.610874 2_1:-19784910 None:intergenic
AATAGAGATGGTATGAGTCA+AGG 0.612883 2_1:+19785309 Msa0200190:three_prime_UTR
TCAACCTTCGAAGATACAAA+CGG 0.613662 2_1:-19784534 None:intergenic
AATCAAACCTGTGCGAATCG+AGG 0.614923 2_1:+19782649 Msa0200190:CDS
TCAACTTCACCATCAAACTC+CGG 0.617812 2_1:+19781831 Msa0200190:CDS
TACCCGGAGTGCATCTAAGA+GGG 0.617885 2_1:-19785044 None:intergenic
CATCAAAAGTCCAATGATTG+GGG 0.619687 2_1:+19785162 Msa0200190:three_prime_UTR
GTGCATATGCCTGATATGGA+TGG 0.621582 2_1:+19782402 Msa0200190:CDS
CAGACTCGACAAAATAGAGA+TGG 0.622886 2_1:+19785297 Msa0200190:three_prime_UTR
TGGAAATGATGAATGTTCCT+GGG 0.628797 2_1:+19783247 Msa0200190:CDS
GAAGAATATATGGCAGCATG+TGG 0.629609 2_1:+19782676 Msa0200190:CDS
TTCGGGAATTAAAGGATTAG+GGG 0.632056 2_1:+19785110 Msa0200190:three_prime_UTR
TTGTATTTCTCGCAGCACAA+AGG 0.634173 2_1:+19785669 Msa0200190:intron
CCACGATGGCCTAAAATCGA+CGG 0.634519 2_1:-19785258 None:intergenic
AAGGCAAGAGCTCAAAGAAG+AGG 0.635070 2_1:+19782810 Msa0200190:CDS
TCGGGAATTAAAGGATTAGG+GGG 0.636049 2_1:+19785111 Msa0200190:three_prime_UTR
ACGCCAGAGTCAATATTTGG+AGG 0.638077 2_1:+19784604 Msa0200190:three_prime_UTR
TCAATAGACCAAACAACTCG+AGG 0.639848 2_1:-19782887 None:intergenic
GGAGTTTGATGGTGAAGTTG+AGG 0.651951 2_1:-19781829 None:intergenic
GGAGACCCGCCGGAACTGGT+CGG 0.659759 2_1:-19781865 None:intergenic
CAAGAGCTCAAAGAAGAGGA+GGG 0.663100 2_1:+19782814 Msa0200190:CDS
TACCAAATGCAAGAGAGCTG+AGG 0.664673 2_1:+19782323 Msa0200190:CDS
GAGTGATGATTAGATTCATG+TGG 0.665667 2_1:-19785813 None:intergenic
GTACTGCTACTGGATATACC+CGG 0.685345 2_1:-19785060 None:intergenic
ATGGCTCAGTCTCAACCAAG+AGG 0.702081 2_1:+19784925 Msa0200190:three_prime_UTR
GTAATGAAAGGTGTGACACA+CGG 0.719143 2_1:+19782611 Msa0200190:CDS
ATAAACTGGAATGTTGTCTG+CGG 0.732217 2_1:-19785733 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCAGAACTAAAAATAAAAAT+GGG - chr2_1:19782111-19782130 None:intergenic 15.0%
!! ATCAGAACTAAAAATAAAAA+TGG - chr2_1:19782112-19782131 None:intergenic 15.0%
!!! AGAAATATTTAAGATTCTAT+GGG - chr2_1:19783128-19783147 None:intergenic 15.0%
!! TTTAACAATAACAAAAATAG+AGG - chr2_1:19783333-19783352 None:intergenic 15.0%
!! TAAAGCCTATTTAATTAAAT+AGG + chr2_1:19783599-19783618 Msa0200190:intron 15.0%
!! TTATACATAAAAAGCTTAAT+AGG - chr2_1:19783670-19783689 None:intergenic 15.0%
!!! CATTTTGAAACAATTTTTTT+TGG - chr2_1:19783800-19783819 None:intergenic 15.0%
!! AAATTATCCTTATTAACTAT+TGG - chr2_1:19783927-19783946 None:intergenic 15.0%
!! AATAGTTAATAAGGATAATT+TGG + chr2_1:19783926-19783945 Msa0200190:intron 15.0%
!! TAAATAATGAAATCTTACAA+AGG - chr2_1:19784066-19784085 None:intergenic 15.0%
!!! AATAAATGATTTGTCTATAA+AGG - chr2_1:19785635-19785654 None:intergenic 15.0%
!! TTAGTTTATGTAAAAATTTC+AGG + chr2_1:19785885-19785904 Msa0200190:three_prime_UTR 15.0%
!!! TTTTAACACTAATTCATGAT+GGG - chr2_1:19781997-19782016 None:intergenic 20.0%
!!! TTTTTAACACTAATTCATGA+TGG - chr2_1:19781998-19782017 None:intergenic 20.0%
!! AAAAACTCAATCAAACAAAA+GGG - chr2_1:19782079-19782098 None:intergenic 20.0%
!! AAAAAACTCAATCAAACAAA+AGG - chr2_1:19782080-19782099 None:intergenic 20.0%
!!! TAAGTGTTTTTTTCAACTAT+TGG + chr2_1:19782200-19782219 Msa0200190:intron 20.0%
!! ATATCTTGGAAAAACTTTAA+GGG - chr2_1:19783054-19783073 None:intergenic 20.0%
!! TATATCTTGGAAAAACTTTA+AGG - chr2_1:19783055-19783074 None:intergenic 20.0%
!!! AAATATTTAAGATTCTATGG+GGG - chr2_1:19783126-19783145 None:intergenic 20.0%
!!! GAAATATTTAAGATTCTATG+GGG - chr2_1:19783127-19783146 None:intergenic 20.0%
!!! CAGAAATATTTAAGATTCTA+TGG - chr2_1:19783129-19783148 None:intergenic 20.0%
!!! ATCATTTCCATAATCTTTTT+AGG - chr2_1:19783237-19783256 None:intergenic 20.0%
!! CTTCTAAACTAACTTATTAT+AGG - chr2_1:19783381-19783400 None:intergenic 20.0%
!!! ATAATAAGTTAGTTTAGAAG+TGG + chr2_1:19783381-19783400 Msa0200190:intron 20.0%
!! AGAAAAAGTTATTATTGCAT+TGG - chr2_1:19783840-19783859 None:intergenic 20.0%
!! AAACTAACCAATAGTTAATA+AGG + chr2_1:19783917-19783936 Msa0200190:intron 20.0%
!!! AGTAAAATAGGCTTTTAAAT+AGG + chr2_1:19784169-19784188 Msa0200190:intron 20.0%
!! TTGAAGATCAATTTCATTTA+CGG - chr2_1:19784635-19784654 None:intergenic 20.0%
!! TAAGGAAACATAGATATTTA+GGG - chr2_1:19785607-19785626 None:intergenic 20.0%
! AGCATAATTCAAAAGAAAGA+TGG - chr2_1:19782147-19782166 None:intergenic 25.0%
!! GCTTTTGATATGATTTAGTA+TGG + chr2_1:19782164-19782183 Msa0200190:intron 25.0%
!! AAAGTCTCAAACTTTAACTT+TGG - chr2_1:19782234-19782253 None:intergenic 25.0%
! CTTCTTAGAGAGAATAAAAA+CGG + chr2_1:19782357-19782376 Msa0200190:CDS 25.0%
! TTCTTAGAGAGAATAAAAAC+GGG + chr2_1:19782358-19782377 Msa0200190:CDS 25.0%
!!! TTCAAACTTTTGGAACATAT+TGG + chr2_1:19782426-19782445 Msa0200190:CDS 25.0%
! AAACAATGCTTACTAATAAC+AGG - chr2_1:19782468-19782487 None:intergenic 25.0%
! CAAGGACATAACATAATTAA+GGG - chr2_1:19782968-19782987 None:intergenic 25.0%
! AAATTGATATTGATATGCAG+AGG + chr2_1:19783206-19783225 Msa0200190:intron 25.0%
! CCTGACCTATTTAATTAAAT+AGG - chr2_1:19783607-19783626 None:intergenic 25.0%
! CCTATTTAATTAAATAGGTC+AGG + chr2_1:19783604-19783623 Msa0200190:intron 25.0%
! AACTTCAACGATATCAATAA+TGG - chr2_1:19783701-19783720 None:intergenic 25.0%
! CTAAATAACCAATCATATGA+AGG - chr2_1:19783743-19783762 None:intergenic 25.0%
!!! TTCAAAATGAGTGTCATTTT+AGG - chr2_1:19784008-19784027 None:intergenic 25.0%
!!! AAATGACACTCATTTTGAAA+CGG + chr2_1:19784009-19784028 Msa0200190:intron 25.0%
! ATGTAAGTCTACTACTAAAT+AGG - chr2_1:19784132-19784151 None:intergenic 25.0%
! GTAAGGAAACATAGATATTT+AGG - chr2_1:19785608-19785627 None:intergenic 25.0%
! TTGTCTATAAAGGAAAAGTA+AGG - chr2_1:19785625-19785644 None:intergenic 25.0%
! TTTGATTCTGTTTTGCTTGT+TGG + chr2_1:19782284-19782303 Msa0200190:intron 30.0%
! TTGATTCTGTTTTGCTTGTT+GGG + chr2_1:19782285-19782304 Msa0200190:intron 30.0%
!! TATGGATGGATTCAAACTTT+TGG + chr2_1:19782416-19782435 Msa0200190:CDS 30.0%
!!! ACTTTTGGAACATATTGGAT+TGG + chr2_1:19782431-19782450 Msa0200190:CDS 30.0%
!!! TGAATGTGTTTTAGTGATGT+CGG + chr2_1:19782568-19782587 Msa0200190:intron 30.0%
GCAAGGACATAACATAATTA+AGG - chr2_1:19782969-19782988 None:intergenic 30.0%
! TGAAAGAGAACATTTTTGCA+AGG - chr2_1:19782986-19783005 None:intergenic 30.0%
!! TCATCTTTGTTTGTTTTCCA+CGG + chr2_1:19783003-19783022 Msa0200190:intron 30.0%
! TTGGAAAAACTTTAAGGGAA+TGG - chr2_1:19783049-19783068 None:intergenic 30.0%
GAATCACAAGGATTATATCT+TGG - chr2_1:19783068-19783087 None:intergenic 30.0%
AACAATCTTGTAGAATCACA+AGG - chr2_1:19783080-19783099 None:intergenic 30.0%
! ATAAGTTAGTTTAGAAGTGG+TGG + chr2_1:19783384-19783403 Msa0200190:intron 30.0%
!! TCGTACAAACTGATTTTGTA+AGG + chr2_1:19783417-19783436 Msa0200190:intron 30.0%
!! CGTACAAACTGATTTTGTAA+GGG + chr2_1:19783418-19783437 Msa0200190:intron 30.0%
CCTATATAAGATCTCATGTA+GGG + chr2_1:19783473-19783492 Msa0200190:intron 30.0%
CTATATAAGATCTCATGTAG+GGG + chr2_1:19783474-19783493 Msa0200190:intron 30.0%
TAATTAAATAGGTCAGGCTA+AGG + chr2_1:19783610-19783629 Msa0200190:intron 30.0%
ACATAAAAAGCTTAATAGGC+CGG - chr2_1:19783666-19783685 None:intergenic 30.0%
CACATATATTCATGTGCTTT+AGG + chr2_1:19784090-19784109 Msa0200190:intron 30.0%
AAATAGGCCAAAATTTACGA+AGG - chr2_1:19784116-19784135 None:intergenic 30.0%
!! GCTAGACTTTAGAGTAAAAT+AGG + chr2_1:19784157-19784176 Msa0200190:intron 30.0%
! TAGGCTTTTAAATAGGCTTT+CGG + chr2_1:19784176-19784195 Msa0200190:intron 30.0%
!!! AGGCCTATAATTTTTGAAGT+AGG + chr2_1:19784271-19784290 Msa0200190:intron 30.0%
TGGCCTACTTCAAAAATTAT+AGG - chr2_1:19784277-19784296 None:intergenic 30.0%
!!! TATAATTTTTGAAGTAGGCC+AGG + chr2_1:19784276-19784295 Msa0200190:intron 30.0%
ACTGTAACTCTTAACATTCA+AGG - chr2_1:19784449-19784468 None:intergenic 30.0%
AAGATCAATTTCATTTACGG+AGG - chr2_1:19784632-19784651 None:intergenic 30.0%
!!! AATCTTAGTTTTGAGTTCTC+AGG - chr2_1:19784727-19784746 None:intergenic 30.0%
GAGAACTCAAAACTAAGATT+TGG + chr2_1:19784727-19784746 Msa0200190:three_prime_UTR 30.0%
TCAAAACTAAGATTTGGAGA+TGG + chr2_1:19784733-19784752 Msa0200190:three_prime_UTR 30.0%
CAAAACTAAGATTTGGAGAT+GGG + chr2_1:19784734-19784753 Msa0200190:three_prime_UTR 30.0%
ATACAAATTCTCATGCTACA+AGG + chr2_1:19784965-19784984 Msa0200190:three_prime_UTR 30.0%
! CTATTGACTGATATTCCATT+TGG - chr2_1:19785021-19785040 None:intergenic 30.0%
TTGATCTCAATGTTTGTACT+CGG - chr2_1:19785508-19785527 None:intergenic 30.0%
! GATAATGAGTTTGACTTAGA+TGG + chr2_1:19785705-19785724 Msa0200190:three_prime_UTR 30.0%
TAACAGGATCTGTTATAAAC+TGG - chr2_1:19785750-19785769 None:intergenic 30.0%
TAGTTGCCAATGTAAATAGT+TGG + chr2_1:19785786-19785805 Msa0200190:three_prime_UTR 30.0%
ATGTAAAAATTTCAGGCTTC+AGG + chr2_1:19785892-19785911 Msa0200190:three_prime_UTR 30.0%
TGTAAAAATTTCAGGCTTCA+GGG + chr2_1:19785893-19785912 Msa0200190:three_prime_UTR 30.0%
CATCATCAACAACAAGTACA+CGG - chr2_1:19781889-19781908 None:intergenic 35.0%
! AAAAACGGAGCATTCTTTCA+AGG - chr2_1:19781931-19781950 None:intergenic 35.0%
AAGATTGAAGACCTTCATAG+AGG - chr2_1:19781955-19781974 None:intergenic 35.0%
AGTTTGAATCCATCCATATC+AGG - chr2_1:19782414-19782433 None:intergenic 35.0%
! GGAAAAAACGTGGTAATGAA+AGG + chr2_1:19782599-19782618 Msa0200190:CDS 35.0%
!! TCGAGGCTTTGAAGAATATA+TGG + chr2_1:19782666-19782685 Msa0200190:CDS 35.0%
TTCGGAAGAGAAAGAATAAC+TGG + chr2_1:19782699-19782718 Msa0200190:CDS 35.0%
AGATGAGTAATTAGCATCAG+TGG - chr2_1:19782780-19782799 None:intergenic 35.0%
TACTCATCTTCAACGAATGA+AGG + chr2_1:19782791-19782810 Msa0200190:CDS 35.0%
AAGAAGATGAAGCAGATGAT+AGG + chr2_1:19782840-19782859 Msa0200190:CDS 35.0%
AGAAGATGAAGCAGATGATA+GGG + chr2_1:19782841-19782860 Msa0200190:CDS 35.0%
TGAGCTTCATCAACAGTTTA+TGG + chr2_1:19782907-19782926 Msa0200190:CDS 35.0%
TATGGCTGTTGTGAATCAAT+TGG + chr2_1:19782925-19782944 Msa0200190:CDS 35.0%
ATGGCTGTTGTGAATCAATT+GGG + chr2_1:19782926-19782945 Msa0200190:CDS 35.0%
TAAGATTCTATGGGGGAAAA+GGG - chr2_1:19783119-19783138 None:intergenic 35.0%
TTAAGATTCTATGGGGGAAA+AGG - chr2_1:19783120-19783139 None:intergenic 35.0%
GGCTGTTCCTAAAAAGATTA+TGG + chr2_1:19783227-19783246 Msa0200190:CDS 35.0%
ATGGAAATGATGAATGTTCC+TGG + chr2_1:19783246-19783265 Msa0200190:CDS 35.0%
TGGAAATGATGAATGTTCCT+GGG + chr2_1:19783247-19783266 Msa0200190:CDS 35.0%
ATATCATACCTGAAGATGAC+TGG - chr2_1:19783292-19783311 None:intergenic 35.0%
CAAAATCAGTTTGTACGATG+AGG - chr2_1:19783415-19783434 None:intergenic 35.0%
GCCTATATAAGATCTCATGT+AGG + chr2_1:19783472-19783491 Msa0200190:intron 35.0%
CCCTACATGAGATCTTATAT+AGG - chr2_1:19783476-19783495 None:intergenic 35.0%
CTTTATAAAAAGCTTGAGCC+TGG - chr2_1:19783584-19783603 None:intergenic 35.0%
AACTCAAGCCTTCATATGAT+TGG + chr2_1:19783732-19783751 Msa0200190:intron 35.0%
!! AAGCGACACTCATTTTGAAA+CGG - chr2_1:19783774-19783793 None:intergenic 35.0%
! TGTGCAGAGTATTAATTGGA+GGG - chr2_1:19783874-19783893 None:intergenic 35.0%
! GTAGTGTGCAGAGTATTAAT+TGG - chr2_1:19783878-19783897 None:intergenic 35.0%
! CTTTAGGCCTTCGTAAATTT+TGG + chr2_1:19784106-19784125 Msa0200190:intron 35.0%
! AGGCTTTTAAATAGGCTTTC+GGG + chr2_1:19784177-19784196 Msa0200190:intron 35.0%
GTAACTCTTAACATTCAAGG+TGG - chr2_1:19784446-19784465 None:intergenic 35.0%
AATACCGTTTGTATCTTCGA+AGG + chr2_1:19784530-19784549 Msa0200190:CDS 35.0%
TCAACCTTCGAAGATACAAA+CGG - chr2_1:19784537-19784556 None:intergenic 35.0%
! ATTCAAGTTACTTTGCTGCT+GGG - chr2_1:19784569-19784588 None:intergenic 35.0%
! TATTCAAGTTACTTTGCTGC+TGG - chr2_1:19784570-19784589 None:intergenic 35.0%
TGAAAATTGGCAAACTGCTT+TGG - chr2_1:19784826-19784845 None:intergenic 35.0%
GTAGGTTGCATTAAAGATGA+TGG - chr2_1:19784994-19785013 None:intergenic 35.0%
TCATCTTTAATGCAACCTAC+AGG + chr2_1:19784994-19785013 Msa0200190:three_prime_UTR 35.0%
ATATCCAGTAGCAGTACAAA+AGG + chr2_1:19785066-19785085 Msa0200190:three_prime_UTR 35.0%
!! TACAAAAGGCGTGTTTTATG+CGG + chr2_1:19785080-19785099 Msa0200190:three_prime_UTR 35.0%
ACTTCGGGAATTAAAGGATT+AGG + chr2_1:19785108-19785127 Msa0200190:three_prime_UTR 35.0%
CTTCGGGAATTAAAGGATTA+GGG + chr2_1:19785109-19785128 Msa0200190:three_prime_UTR 35.0%
TTCGGGAATTAAAGGATTAG+GGG + chr2_1:19785110-19785129 Msa0200190:three_prime_UTR 35.0%
TCCATCAAAAGTCCAATGAT+TGG + chr2_1:19785160-19785179 Msa0200190:three_prime_UTR 35.0%
CCATCAAAAGTCCAATGATT+GGG + chr2_1:19785161-19785180 Msa0200190:three_prime_UTR 35.0%
CATCAAAAGTCCAATGATTG+GGG + chr2_1:19785162-19785181 Msa0200190:three_prime_UTR 35.0%
! ATTTGCTAATCCCCAATCAT+TGG - chr2_1:19785175-19785194 None:intergenic 35.0%
CAACATGCAAATCCTTTACA+AGG + chr2_1:19785219-19785238 Msa0200190:three_prime_UTR 35.0%
AGATCAATGTTGCCTTGTAA+AGG - chr2_1:19785234-19785253 None:intergenic 35.0%
AATAGAGATGGTATGAGTCA+AGG + chr2_1:19785309-19785328 Msa0200190:three_prime_UTR 35.0%
! ACCTTTTGTCGACAATTCAT+TGG + chr2_1:19785364-19785383 Msa0200190:three_prime_UTR 35.0%
TCCAATGAATTGTCGACAAA+AGG - chr2_1:19785368-19785387 None:intergenic 35.0%
GTGATGAAACATTATGTGTG+AGG - chr2_1:19785448-19785467 None:intergenic 35.0%
! AGATATTTAGGGTCAACAGA+AGG - chr2_1:19785596-19785615 None:intergenic 35.0%
ATAAACTGGAATGTTGTCTG+CGG - chr2_1:19785736-19785755 None:intergenic 35.0%
TGATTAGAAAGAGAGCTAAC+AGG - chr2_1:19785766-19785785 None:intergenic 35.0%
GGAACACCAACTATTTACAT+TGG - chr2_1:19785795-19785814 None:intergenic 35.0%
GAGTGATGATTAGATTCATG+TGG - chr2_1:19785816-19785835 None:intergenic 35.0%
TCAACTTCACCATCAAACTC+CGG + chr2_1:19781831-19781850 Msa0200190:CDS 40.0%
GCATTCTTTCAAGGATCATG+AGG - chr2_1:19781922-19781941 None:intergenic 40.0%
GACCTTCATAGAGGCAAAAA+CGG - chr2_1:19781946-19781965 None:intergenic 40.0%
! GGAACATATTGGATTGGAGA+TGG + chr2_1:19782437-19782456 Msa0200190:CDS 40.0%
! GTAATGAAAGGTGTGACACA+CGG + chr2_1:19782611-19782630 Msa0200190:CDS 40.0%
GAAGAATATATGGCAGCATG+TGG + chr2_1:19782676-19782695 Msa0200190:CDS 40.0%
ATATATGGCAGCATGTGGTT+CGG + chr2_1:19782681-19782700 Msa0200190:CDS 40.0%
! TGGAAAGATCTGAAGCAATC+TGG + chr2_1:19782719-19782738 Msa0200190:CDS 40.0%
! AAAGATCTGAAGCAATCTGG+TGG + chr2_1:19782722-19782741 Msa0200190:intron 40.0%
TCAATAGACCAAACAACTCG+AGG - chr2_1:19782890-19782909 None:intergenic 40.0%
GGTCATATCTCATCTAACGT+GGG - chr2_1:19783360-19783379 None:intergenic 40.0%
AGGTCATATCTCATCTAACG+TGG - chr2_1:19783361-19783380 None:intergenic 40.0%
TATAAGATCTCATGTAGGGG+TGG + chr2_1:19783477-19783496 Msa0200190:intron 40.0%
ATAAGATCTCATGTAGGGGT+GGG + chr2_1:19783478-19783497 Msa0200190:intron 40.0%
CTTTAAATAGGCTAGGCCAT+AGG - chr2_1:19783529-19783548 None:intergenic 40.0%
!! AGCTAGGCTTTAAATAGGCT+AGG - chr2_1:19783536-19783555 None:intergenic 40.0%
! AGCCTATTTAAAGCCTAGCT+CGG + chr2_1:19783536-19783555 Msa0200190:intron 40.0%
! GCCTTGTTAATAAACGAGCT+GGG - chr2_1:19783562-19783581 None:intergenic 40.0%
AAAAAGCCTATTAAGCCCGT+CGG + chr2_1:19783639-19783658 Msa0200190:intron 40.0%
!!! GACACTCATTTTGAAACGGA+GGG - chr2_1:19784013-19784032 None:intergenic 40.0%
! GTGTGCAGAGTATTAATTGG+AGG - chr2_1:19783875-19783894 None:intergenic 40.0%
!!! TGACACTCATTTTGAAACGG+AGG + chr2_1:19784012-19784031 Msa0200190:intron 40.0%
!!! GACACTCATTTTGAAACGGA+GGG + chr2_1:19784013-19784032 Msa0200190:intron 40.0%
GGAGGGAGTAGTAGATATAT+TGG + chr2_1:19784030-19784049 Msa0200190:intron 40.0%
GCTTCATCAGGCCTTTAAAT+AGG + chr2_1:19784199-19784218 Msa0200190:intron 40.0%
AAAAATTATAGGCCTGAGCC+TGG - chr2_1:19784266-19784285 None:intergenic 40.0%
ATGTCCACTTATCAGTGTGA+GGG + chr2_1:19784399-19784418 Msa0200190:intron 40.0%
TTGTATCTTCGAAGGTTGAG+TGG + chr2_1:19784538-19784557 Msa0200190:CDS 40.0%
AGTACGCCAGAGTCAATATT+TGG + chr2_1:19784601-19784620 Msa0200190:three_prime_UTR 40.0%
!! GTTCCTCCAAATATTGACTC+TGG - chr2_1:19784610-19784629 None:intergenic 40.0%
ACATATGAGCAGCAGTAAAC+CGG + chr2_1:19784765-19784784 Msa0200190:three_prime_UTR 40.0%
AAACCGGTGAACTTTCTTCA+CGG + chr2_1:19784781-19784800 Msa0200190:three_prime_UTR 40.0%
ATTCCGTGAAGAAAGTTCAC+CGG - chr2_1:19784787-19784806 None:intergenic 40.0%
! GCTTTGGCTCAATGTTGATA+GGG - chr2_1:19784810-19784829 None:intergenic 40.0%
! TGCTTTGGCTCAATGTTGAT+AGG - chr2_1:19784811-19784830 None:intergenic 40.0%
TTGAGCAGACTGGTGAAAAT+TGG - chr2_1:19784839-19784858 None:intergenic 40.0%
TCATTTAGCATCTGACCTCT+TGG - chr2_1:19784943-19784962 None:intergenic 40.0%
GATATTCCATTTGGTCCTGT+AGG - chr2_1:19785012-19785031 None:intergenic 40.0%
! GTTTTATGCGGAAGCTACTT+CGG + chr2_1:19785092-19785111 Msa0200190:three_prime_UTR 40.0%
! TTTTATGCGGAAGCTACTTC+GGG + chr2_1:19785093-19785112 Msa0200190:three_prime_UTR 40.0%
GAAGCTACTTCGGGAATTAA+AGG + chr2_1:19785102-19785121 Msa0200190:three_prime_UTR 40.0%
TCGGGAATTAAAGGATTAGG+GGG + chr2_1:19785111-19785130 Msa0200190:three_prime_UTR 40.0%
! CCCAATCATTGGACTTTTGA+TGG - chr2_1:19785164-19785183 None:intergenic 40.0%
! GATTGGGGATTAGCAAATAC+AGG + chr2_1:19785177-19785196 Msa0200190:three_prime_UTR 40.0%
!! GTTGAGAAGCATCATATGTC+AGG - chr2_1:19785203-19785222 None:intergenic 40.0%
! CTCTATTTTGTCGAGTCTGT+TGG - chr2_1:19785296-19785315 None:intergenic 40.0%
CAGACTCGACAAAATAGAGA+TGG + chr2_1:19785297-19785316 Msa0200190:three_prime_UTR 40.0%
CAAGGTGATCACCATAATGT+TGG + chr2_1:19785327-19785346 Msa0200190:three_prime_UTR 40.0%
!! TGAGGTGTTGACCAACATTA+TGG - chr2_1:19785341-19785360 None:intergenic 40.0%
! TTGTCGACAAAAGGTGTATG+AGG - chr2_1:19785359-19785378 None:intergenic 40.0%
AGAAGAGAATCCTGAGACAT+TGG + chr2_1:19785387-19785406 Msa0200190:intron 40.0%
TCATGATCCTCTTACTACGA+GGG - chr2_1:19785419-19785438 None:intergenic 40.0%
!! TGACTGACTTGATCAGAGAA+GGG - chr2_1:19785532-19785551 None:intergenic 40.0%
!! TTGACTGACTTGATCAGAGA+AGG - chr2_1:19785533-19785552 None:intergenic 40.0%
AACAGAAGGAAAAACCAACC+TGG - chr2_1:19785582-19785601 None:intergenic 40.0%
TTGTATTTCTCGCAGCACAA+AGG + chr2_1:19785669-19785688 Msa0200190:intron 40.0%
TGGTGAAGTTGAGGTTGACA+TGG - chr2_1:19781823-19781842 None:intergenic 45.0%
!! GGAGTTTGATGGTGAAGTTG+AGG - chr2_1:19781832-19781851 None:intergenic 45.0%
ATAAGGTTGCCGGAGTTTGA+TGG - chr2_1:19781843-19781862 None:intergenic 45.0%
CAAGGATCATGAGGCAAGTA+GGG - chr2_1:19781913-19781932 None:intergenic 45.0%
TCAAGGATCATGAGGCAAGT+AGG - chr2_1:19781914-19781933 None:intergenic 45.0%
!! CTCCGTTTTTGCCTCTATGA+AGG + chr2_1:19781941-19781960 Msa0200190:intron 45.0%
TACCAAATGCAAGAGAGCTG+AGG + chr2_1:19782323-19782342 Msa0200190:CDS 45.0%
AACCTCAGCTCTCTTGCATT+TGG - chr2_1:19782328-19782347 None:intergenic 45.0%
GTGCATATGCCTGATATGGA+TGG + chr2_1:19782402-19782421 Msa0200190:CDS 45.0%
! TTAGTGATGTCGGCTGATGA+TGG + chr2_1:19782578-19782597 Msa0200190:intron 45.0%
!! GGCTGATGATGGAAAAAACG+TGG + chr2_1:19782589-19782608 Msa0200190:CDS 45.0%
AATCAAACCTGTGCGAATCG+AGG + chr2_1:19782649-19782668 Msa0200190:CDS 45.0%
AAGGCAAGAGCTCAAAGAAG+AGG + chr2_1:19782810-19782829 Msa0200190:CDS 45.0%
CAAGAGCTCAAAGAAGAGGA+GGG + chr2_1:19782814-19782833 Msa0200190:CDS 45.0%
! CAAAGAAGCCTCGAGTTGTT+TGG + chr2_1:19782879-19782898 Msa0200190:CDS 45.0%
GTACGTTAGATGAGATACCG+TGG - chr2_1:19783023-19783042 None:intergenic 45.0%
AAACGTTGCCAGTCATCTTC+AGG + chr2_1:19783281-19783300 Msa0200190:intron 45.0%
GCCCAGCTCGTTTATTAACA+AGG + chr2_1:19783558-19783577 Msa0200190:intron 45.0%
!! GGCCTTGTTAATAAACGAGC+TGG - chr2_1:19783563-19783582 None:intergenic 45.0%
GCTCGTTTATTAACAAGGCC+AGG + chr2_1:19783563-19783582 Msa0200190:intron 45.0%
AAAAGCCTATTAAGCCCGTC+GGG + chr2_1:19783640-19783659 Msa0200190:intron 45.0%
!!! CGACACTCATTTTGAAACGG+AGG - chr2_1:19783771-19783790 None:intergenic 45.0%
ATCAGGCCTTTAAATAGGCC+AGG + chr2_1:19784204-19784223 Msa0200190:intron 45.0%
CCTCGAAATTGAGCCTATGA+CGG + chr2_1:19784232-19784251 Msa0200190:intron 45.0%
CTCGAAATTGAGCCTATGAC+GGG + chr2_1:19784233-19784252 Msa0200190:intron 45.0%
!! TTGAGCCTATGACGGGTAAT+AGG + chr2_1:19784240-19784259 Msa0200190:intron 45.0%
!!! TTTTTGAAGTAGGCCAGGCT+TGG + chr2_1:19784281-19784300 Msa0200190:intron 45.0%
!!! TTTTGAAGTAGGCCAGGCTT+GGG + chr2_1:19784282-19784301 Msa0200190:intron 45.0%
TAGCCTATTTCCACCCTCAT+GGG + chr2_1:19784327-19784346 Msa0200190:intron 45.0%
CACACTGATAAGTGGACATC+TGG - chr2_1:19784398-19784417 None:intergenic 45.0%
GATGTCCACTTATCAGTGTG+AGG + chr2_1:19784398-19784417 Msa0200190:intron 45.0%
TGTCCACTTATCAGTGTGAG+GGG + chr2_1:19784400-19784419 Msa0200190:intron 45.0%
ACGCCAGAGTCAATATTTGG+AGG + chr2_1:19784604-19784623 Msa0200190:three_prime_UTR 45.0%
CTTCAAACCCTTTCAGCTTC+TGG + chr2_1:19784649-19784668 Msa0200190:three_prime_UTR 45.0%
ATGCAACCTACAGGACCAAA+TGG + chr2_1:19785003-19785022 Msa0200190:three_prime_UTR 45.0%
TTTCCCTCTTAGATGCACTC+CGG + chr2_1:19785041-19785060 Msa0200190:three_prime_UTR 45.0%
GTACTGCTACTGGATATACC+CGG - chr2_1:19785063-19785082 None:intergenic 45.0%
GGATAGAGGAAAAACCACGA+TGG - chr2_1:19785275-19785294 None:intergenic 45.0%
TTGTCGAGTCTGTTGGATAG+AGG - chr2_1:19785289-19785308 None:intergenic 45.0%
CATGATCCTCTTACTACGAG+GGG - chr2_1:19785418-19785437 None:intergenic 45.0%
CTCATGATCCTCTTACTACG+AGG - chr2_1:19785420-19785439 None:intergenic 45.0%
CGTAGTAAGAGGATCATGAG+CGG + chr2_1:19785420-19785439 Msa0200190:intron 45.0%
TTCATCACCTCACACCACAT+CGG + chr2_1:19785460-19785479 Msa0200190:intron 45.0%
!! GACTGACTTGATCAGAGAAG+GGG - chr2_1:19785531-19785550 None:intergenic 45.0%
CTCTGATCAAGTCAGTCAAG+AGG + chr2_1:19785534-19785553 Msa0200190:three_prime_UTR 45.0%
! CATGAGCGTATTTCTGAACC+AGG + chr2_1:19785561-19785580 Msa0200190:three_prime_UTR 45.0%
! AGCGTATTTCTGAACCAGGT+TGG + chr2_1:19785565-19785584 Msa0200190:intron 45.0%
!!! TTTTTTTTTTTTTATTGATT+TGG + chr2_1:19782486-19782505 Msa0200190:intron 5.0%
ACCTTATCTCCGACCAGTTC+CGG + chr2_1:19781856-19781875 Msa0200190:CDS 50.0%
CGACGTGCATATGCCTGATA+TGG + chr2_1:19782398-19782417 Msa0200190:CDS 50.0%
! TTCAAAGCCTCGATTCGCAC+AGG - chr2_1:19782659-19782678 None:intergenic 50.0%
GCAAGAGCTCAAAGAAGAGG+AGG + chr2_1:19782813-19782832 Msa0200190:CDS 50.0%
! ACGTTTTCTCTAGTGAGCCC+AGG - chr2_1:19783267-19783286 None:intergenic 50.0%
!! GGCCGAGCTAGGCTTTAAAT+AGG - chr2_1:19783541-19783560 None:intergenic 50.0%
ATAGGCTTTCGGGCTTCATC+AGG + chr2_1:19784187-19784206 Msa0200190:intron 50.0%
ATAGGCTCAATTTCGAGGCC+TGG - chr2_1:19784230-19784249 None:intergenic 50.0%
CCGTCATAGGCTCAATTTCG+AGG - chr2_1:19784235-19784254 None:intergenic 50.0%
CATGAGGGTGGAAATAGGCT+AGG - chr2_1:19784328-19784347 None:intergenic 50.0%
CTAGCCTATTTCCACCCTCA+TGG + chr2_1:19784326-19784345 Msa0200190:intron 50.0%
GGCTATCAAGTCACCGCTAT+TGG + chr2_1:19784355-19784374 Msa0200190:intron 50.0%
GTCCACTTATCAGTGTGAGG+GGG + chr2_1:19784401-19784420 Msa0200190:intron 50.0%
TGACTCTGGCGTACTCATGA+AGG - chr2_1:19784596-19784615 None:intergenic 50.0%
GAACTGGCCAGAAGCTGAAA+GGG - chr2_1:19784659-19784678 None:intergenic 50.0%
AGAACTGGCCAGAAGCTGAA+AGG - chr2_1:19784660-19784679 None:intergenic 50.0%
TTCAGCTTCTGGCCAGTTCT+CGG + chr2_1:19784660-19784679 Msa0200190:three_prime_UTR 50.0%
CTAGACTTTGCACCGAGAAC+TGG - chr2_1:19784675-19784694 None:intergenic 50.0%
! AAGTCCTGCTGCTTGAAGCT+TGG - chr2_1:19784698-19784717 None:intergenic 50.0%
CACCAGTCTGCTCAATCCTT+TGG + chr2_1:19784844-19784863 Msa0200190:three_prime_UTR 50.0%
TGCCAAAGGATTGAGCAGAC+TGG - chr2_1:19784849-19784868 None:intergenic 50.0%
TCGCATATTTGCGCTGCCAA+AGG - chr2_1:19784863-19784882 None:intergenic 50.0%
! AAGCCCTTGGTTGATGCAGA+TGG + chr2_1:19784906-19784925 Msa0200190:three_prime_UTR 50.0%
GAGCCATCTGCATCAACCAA+GGG - chr2_1:19784912-19784931 None:intergenic 50.0%
TGAGCCATCTGCATCAACCA+AGG - chr2_1:19784913-19784932 None:intergenic 50.0%
ATGGCTCAGTCTCAACCAAG+AGG + chr2_1:19784925-19784944 Msa0200190:three_prime_UTR 50.0%
TTCCCTCTTAGATGCACTCC+GGG + chr2_1:19785042-19785061 Msa0200190:three_prime_UTR 50.0%
TACCCGGAGTGCATCTAAGA+GGG - chr2_1:19785047-19785066 None:intergenic 50.0%
ATACCCGGAGTGCATCTAAG+AGG - chr2_1:19785048-19785067 None:intergenic 50.0%
! CACGCCTTTTGTACTGCTAC+TGG - chr2_1:19785073-19785092 None:intergenic 50.0%
!!! GATCTCTCGCCGTCGATTTT+AGG + chr2_1:19785249-19785268 Msa0200190:three_prime_UTR 50.0%
CCACGATGGCCTAAAATCGA+CGG - chr2_1:19785261-19785280 None:intergenic 50.0%
! AGGGGTTGATCCAATGTCTC+AGG - chr2_1:19785400-19785419 None:intergenic 50.0%
GATCAACCCCTCGTAGTAAG+AGG + chr2_1:19785409-19785428 Msa0200190:intron 50.0%
CTGGTCGGAGATAAGGTTGC+CGG - chr2_1:19781853-19781872 None:intergenic 55.0%
TTATCTCCGACCAGTTCCGG+CGG + chr2_1:19781859-19781878 Msa0200190:CDS 55.0%
TCTCATGTAGGGGTGGGAAC+AGG + chr2_1:19783484-19783503 Msa0200190:intron 55.0%
GCCTTTAAATAGGCCAGGCC+AGG + chr2_1:19784209-19784228 Msa0200190:intron 55.0%
GCCTGGCCTGGCCTATTTAA+AGG - chr2_1:19784213-19784232 None:intergenic 55.0%
CCTGGCCTATTACCCGTCAT+AGG - chr2_1:19784248-19784267 None:intergenic 55.0%
!! CCTATGACGGGTAATAGGCC+AGG + chr2_1:19784245-19784264 Msa0200190:intron 55.0%
AGGGTGGAAATAGGCTAGGC+CGG - chr2_1:19784324-19784343 None:intergenic 55.0%
TGGCCCATGAGGGTGGAAAT+AGG - chr2_1:19784333-19784352 None:intergenic 55.0%
TGATAGCCAGTGGCCCATGA+GGG - chr2_1:19784343-19784362 None:intergenic 55.0%
! TTGATAGCCAGTGGCCCATG+AGG - chr2_1:19784344-19784363 None:intergenic 55.0%
! AGCGGTGACTTGATAGCCAG+TGG - chr2_1:19784353-19784372 None:intergenic 55.0%
! ATCTGGAGTGTGACTCGAGC+GGG - chr2_1:19784381-19784400 None:intergenic 55.0%
! CATCTGGAGTGTGACTCGAG+CGG - chr2_1:19784382-19784401 None:intergenic 55.0%
CACCCCCTCACACTGATAAG+TGG - chr2_1:19784406-19784425 None:intergenic 55.0%
CTAGCCAAGCTTCAAGCAGC+AGG + chr2_1:19784691-19784710 Msa0200190:three_prime_UTR 55.0%
CTGCTGCAAAGAGAAGCCCT+TGG + chr2_1:19784893-19784912 Msa0200190:three_prime_UTR 55.0%
!!! CCGTCGATTTTAGGCCATCG+TGG + chr2_1:19785258-19785277 Msa0200190:three_prime_UTR 55.0%
ACCTCACACCACATCGGACA+GGG + chr2_1:19785466-19785485 Msa0200190:intron 55.0%
CACTCTTACCCTGTCCGATG+TGG - chr2_1:19785477-19785496 None:intergenic 55.0%
! TTCTCGCAGCACAAAGGCGT+TGG + chr2_1:19785675-19785694 Msa0200190:intron 55.0%
GCCGGAACTGGTCGGAGATA+AGG - chr2_1:19781860-19781879 None:intergenic 60.0%
TATCTCCGACCAGTTCCGGC+GGG + chr2_1:19781860-19781879 Msa0200190:CDS 60.0%
ACAAGTACACGGAGACCCGC+CGG - chr2_1:19781878-19781897 None:intergenic 60.0%
TAAACGAGCTGGGCCGAGCT+AGG - chr2_1:19783552-19783571 None:intergenic 60.0%
AGCTTAATAGGCCGGCCCGA+CGG - chr2_1:19783658-19783677 None:intergenic 60.0%
CTCAATTTCGAGGCCTGGCC+TGG - chr2_1:19784225-19784244 None:intergenic 60.0%
ACGGGTAATAGGCCAGGCTC+AGG + chr2_1:19784251-19784270 Msa0200190:intron 60.0%
! GGCTTTGTAAGGCCCAAGCC+TGG - chr2_1:19784297-19784316 None:intergenic 60.0%
GGCTTGGGCCTTACAAAGCC+CGG + chr2_1:19784297-19784316 Msa0200190:intron 60.0%
GGGTGGAAATAGGCTAGGCC+GGG - chr2_1:19784323-19784342 None:intergenic 60.0%
TTTCCACCCTCATGGGCCAC+TGG + chr2_1:19784334-19784353 Msa0200190:intron 60.0%
TGACTCGAGCGGGCCAATAG+CGG - chr2_1:19784371-19784390 None:intergenic 60.0%
CACCTCACACCACATCGGAC+AGG + chr2_1:19785465-19785484 Msa0200190:intron 60.0%
ACCCTGTCCGATGTGGTGTG+AGG - chr2_1:19785470-19785489 None:intergenic 60.0%
TGTAGGGGTGGGAACAGGCC+AGG + chr2_1:19783489-19783508 Msa0200190:intron 65.0%
GCTAGGCCATAGGCCCCTGT+CGG - chr2_1:19783519-19783538 None:intergenic 65.0%
GCCTATTAAGCCCGTCGGGC+CGG + chr2_1:19783644-19783663 Msa0200190:intron 65.0%
GCTTAATAGGCCGGCCCGAC+GGG - chr2_1:19783657-19783676 None:intergenic 65.0%
GAAATAGGCTAGGCCGGGCC+GGG - chr2_1:19784318-19784337 None:intergenic 65.0%
GGAAATAGGCTAGGCCGGGC+CGG - chr2_1:19784319-19784338 None:intergenic 65.0%
TAGCCAGTGGCCCATGAGGG+TGG - chr2_1:19784340-19784359 None:intergenic 65.0%
GGAGACCCGCCGGAACTGGT+CGG - chr2_1:19781868-19781887 None:intergenic 70.0%
ACACGGAGACCCGCCGGAAC+TGG - chr2_1:19781872-19781891 None:intergenic 70.0%
AACAGGCCAGGCCGTCCGAC+AGG + chr2_1:19783501-19783520 Msa0200190:intron 70.0%
ACAGGCCAGGCCGTCCGACA+GGG + chr2_1:19783502-19783521 Msa0200190:intron 70.0%
GGCCATAGGCCCCTGTCGGA+CGG - chr2_1:19783515-19783534 None:intergenic 70.0%
! GCCGGCCCGACGGGCTTAAT+AGG - chr2_1:19783648-19783667 None:intergenic 70.0%
GGGCCTTACAAAGCCCGGCC+CGG + chr2_1:19784302-19784321 Msa0200190:intron 70.0%
! AGGCCGGGCCGGGCTTTGTA+AGG - chr2_1:19784308-19784327 None:intergenic 70.0%
CAGGCCAGGCCGTCCGACAG+GGG + chr2_1:19783503-19783522 Msa0200190:intron 75.0%
TAGGCCCCTGTCGGACGGCC+TGG - chr2_1:19783510-19783529 None:intergenic 75.0%
GGCCGTCCGACAGGGGCCTA+TGG + chr2_1:19783510-19783529 Msa0200190:intron 75.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 19781792 19785922 19781792 ID=Msa0200190;Name=Msa0200190
chr2_1 mRNA 19781792 19785922 19781792 ID=Msa0200190-mRNA-1;Parent=Msa0200190;Name=Msa0200190-mRNA-1;_AED=0.11;_eAED=0.11;_QI=0|0.5|0.44|1|0.37|0.22|9|1142|247
chr2_1 exon 19781792 19781889 19781792 ID=Msa0200190-mRNA-1:exon:8182;Parent=Msa0200190-mRNA-1
chr2_1 exon 19781937 19781944 19781937 ID=Msa0200190-mRNA-1:exon:8183;Parent=Msa0200190-mRNA-1
chr2_1 exon 19782322 19782474 19782322 ID=Msa0200190-mRNA-1:exon:8184;Parent=Msa0200190-mRNA-1
chr2_1 exon 19782582 19782740 19782582 ID=Msa0200190-mRNA-1:exon:8185;Parent=Msa0200190-mRNA-1
chr2_1 exon 19782750 19782956 19782750 ID=Msa0200190-mRNA-1:exon:8186;Parent=Msa0200190-mRNA-1
chr2_1 exon 19783226 19783291 19783226 ID=Msa0200190-mRNA-1:exon:8187;Parent=Msa0200190-mRNA-1
chr2_1 exon 19784529 19785384 19784529 ID=Msa0200190-mRNA-1:exon:8188;Parent=Msa0200190-mRNA-1
chr2_1 exon 19785483 19785582 19785483 ID=Msa0200190-mRNA-1:exon:8189;Parent=Msa0200190-mRNA-1
chr2_1 exon 19785684 19785922 19785684 ID=Msa0200190-mRNA-1:exon:8190;Parent=Msa0200190-mRNA-1
chr2_1 CDS 19781792 19781889 19781792 ID=Msa0200190-mRNA-1:cds;Parent=Msa0200190-mRNA-1
chr2_1 CDS 19781937 19781944 19781937 ID=Msa0200190-mRNA-1:cds;Parent=Msa0200190-mRNA-1
chr2_1 CDS 19782322 19782474 19782322 ID=Msa0200190-mRNA-1:cds;Parent=Msa0200190-mRNA-1
chr2_1 CDS 19782582 19782740 19782582 ID=Msa0200190-mRNA-1:cds;Parent=Msa0200190-mRNA-1
chr2_1 CDS 19782750 19782956 19782750 ID=Msa0200190-mRNA-1:cds;Parent=Msa0200190-mRNA-1
chr2_1 CDS 19783226 19783291 19783226 ID=Msa0200190-mRNA-1:cds;Parent=Msa0200190-mRNA-1
chr2_1 CDS 19784529 19784581 19784529 ID=Msa0200190-mRNA-1:cds;Parent=Msa0200190-mRNA-1
chr2_1 three_prime_UTR 19784582 19785384 19784582 ID=Msa0200190-mRNA-1:three_prime_utr;Parent=Msa0200190-mRNA-1
chr2_1 three_prime_UTR 19785483 19785582 19785483 ID=Msa0200190-mRNA-1:three_prime_utr;Parent=Msa0200190-mRNA-1
chr2_1 three_prime_UTR 19785684 19785922 19785684 ID=Msa0200190-mRNA-1:three_prime_utr;Parent=Msa0200190-mRNA-1
Gene Sequence

>Msa0200190

ATGAAACTCAACAACAACAACAAAATTTCCATGTCAACCTCAACTTCACCATCAAACTCCGGCAACCTTATCTCCGACCAGTTCCGGCGGGTCTCCGTAATGCTCCTTACCAAATGCAAGAGAGCTGAGGTTGCATTATCACTTCTTAGAGAGAATAAAAACGGGTTCGACATTGTTTTAAGCGACGTGCATATGCCTGATATGGATGGATTCAAACTTTTGGAACATATTGGATTGGAGATGGATCTTCCTGTTATTATGATGTCGGCTGATGATGGAAAAAACGTGGTAATGAAAGGTGTGACACACGGTGCTTGTGATTATCTAATCAAACCTGTGCGAATCGAGGCTTTGAAGAATATATGGCAGCATGTGGTTCGGAAGAGAAAGAATAACTGGAAAGATCTGAAGCAATCTGATGAAAGAGATCGAGAAAAAATTGTGTCCACTGATGCTAATTACTCATCTTCAACGAATGAAGGCAAGAGCTCAAAGAAGAGGAGGGATGAAGAAGATGAAGCAGATGATAGGGATGATTGTTCGACGTCAAAGAAGCCTCGAGTTGTTTGGTCTATTGAGCTTCATCAACAGTTTATGGCTGTTGTGAATCAATTGGGATTAGACAAGGCTGTTCCTAAAAAGATTATGGAAATGATGAATGTTCCTGGGCTCACTAGAGAAAACGTTGCCAAAATACCGTTTGTATCTTCGAAGGTTGAGTGGAGTTTCCCAGCAGCAAAGTAA
Protein sequence

>Msa0200190

MKLNNNNKISMSTSTSPSNSGNLISDQFRRVSVMLLTKCKRAEVALSLLRENKNGFDIVLSDVHMPDMDGFKLLEHIGLEMDLPVIMMSADDGKNVVMKGVTHGACDYLIKPVRIEALKNIWQHVVRKRKNNWKDLKQSDERDREKIVSTDANYSSSTNEGKSSKKRRDEEDEADDRDDCSTSKKPRVVWSIELHQQFMAVVNQLGLDKAVPKKIMEMMNVPGLTRENVAKIPFVSSKVEWSFPAAK*