Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1313300 | sp|P0DH62|Y4407_SELML | 45.690 | 580 | 204 | 11 | 104 | 677 | 5 | 479 | 4.68e-149 | 450 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1313300 | A0A072TXS4 | 98.808 | 755 | 9 | 0 | 1 | 755 | 1 | 755 | 0.0 | 1548 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0008110 | Msa1313300 | 0.812164 | 4.865037e-51 | -8.615850e-47 |
| Msa0016670 | Msa1313300 | 0.859328 | 4.461715e-63 | -8.615850e-47 |
| Msa0017510 | Msa1313300 | 0.800066 | 1.715541e-48 | -8.615850e-47 |
| Msa0021500 | Msa1313300 | 0.801774 | 7.683692e-49 | -8.615850e-47 |
| Msa0027230 | Msa1313300 | 0.805873 | 1.081930e-49 | -8.615850e-47 |
| Msa0028420 | Msa1313300 | 0.815443 | 9.222862e-52 | -8.615850e-47 |
| Msa0032920 | Msa1313300 | -0.838240 | 3.234989e-57 | -8.615850e-47 |
| Msa0032960 | Msa1313300 | -0.813897 | 2.028080e-51 | -8.615850e-47 |
| Msa0034270 | Msa1313300 | 0.809956 | 1.464134e-50 | -8.615850e-47 |
| Msa0034280 | Msa1313300 | 0.814757 | 1.309141e-51 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1313300 | MtrunA17_Chr8g0341881 | 98.808 | 755 | 9 | 0 | 1 | 755 | 1 | 755 | 0.0 | 1548 |
| Msa1313300 | MtrunA17_Chr2g0330471 | 80.174 | 691 | 123 | 8 | 1 | 683 | 1 | 685 | 0.0 | 1099 |
| Msa1313300 | MtrunA17_Chr8g0389251 | 64.148 | 675 | 232 | 7 | 12 | 681 | 11 | 680 | 0.0 | 857 |
| Msa1313300 | MtrunA17_Chr1g0154291 | 45.791 | 594 | 265 | 16 | 143 | 700 | 1 | 573 | 2.65e-147 | 443 |
| Msa1313300 | MtrunA17_Chr3g0125631 | 52.427 | 309 | 145 | 2 | 371 | 678 | 39 | 346 | 1.69e-111 | 342 |
| Msa1313300 | MtrunA17_Chr3g0080901 | 53.311 | 302 | 132 | 5 | 374 | 675 | 98 | 390 | 3.35e-99 | 315 |
| Msa1313300 | MtrunA17_Chr7g0221191 | 49.057 | 318 | 152 | 4 | 392 | 700 | 382 | 698 | 1.96e-97 | 318 |
| Msa1313300 | MtrunA17_Chr3g0144481 | 48.276 | 319 | 153 | 5 | 369 | 682 | 59 | 370 | 2.58e-96 | 306 |
| Msa1313300 | MtrunA17_Chr5g0413511 | 48.580 | 317 | 151 | 4 | 377 | 682 | 25 | 340 | 1.69e-95 | 302 |
| Msa1313300 | MtrunA17_Chr6g0485761 | 48.624 | 327 | 156 | 4 | 370 | 691 | 266 | 585 | 2.79e-94 | 308 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1313300 | AT3G13690.2 | 73.684 | 760 | 172 | 15 | 1 | 748 | 1 | 744 | 0.0 | 1091 |
| Msa1313300 | AT3G13690.1 | 73.684 | 760 | 172 | 15 | 1 | 748 | 1 | 744 | 0.0 | 1091 |
| Msa1313300 | AT3G13690.3 | 73.684 | 760 | 172 | 15 | 1 | 748 | 1 | 744 | 0.0 | 1091 |
| Msa1313300 | AT1G55200.3 | 68.707 | 719 | 173 | 17 | 1 | 710 | 1 | 676 | 0.0 | 951 |
| Msa1313300 | AT1G55200.4 | 68.707 | 719 | 173 | 17 | 1 | 710 | 1 | 676 | 0.0 | 951 |
| Msa1313300 | AT1G55200.1 | 68.707 | 719 | 173 | 17 | 1 | 710 | 1 | 676 | 0.0 | 951 |
| Msa1313300 | AT1G55200.2 | 68.707 | 719 | 173 | 17 | 1 | 710 | 1 | 676 | 0.0 | 951 |
| Msa1313300 | AT5G56790.1 | 60.876 | 662 | 235 | 7 | 28 | 682 | 23 | 667 | 0.0 | 784 |
| Msa1313300 | AT1G68690.1 | 49.555 | 337 | 157 | 4 | 344 | 676 | 323 | 650 | 1.43e-103 | 333 |
| Msa1313300 | AT1G68690.3 | 49.555 | 337 | 157 | 4 | 344 | 676 | 323 | 650 | 1.43e-103 | 333 |
Find 235 sgRNAs with CRISPR-Local
Find 420 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACGGTTATGGAAATGAG+GGG | 0.718438 | 8_4:-11188921 | None:intergenic |
| AAAAGCAATTCCGTGATCCA+CGG | 0.626544 | 8_4:-11191572 | None:intergenic |
| AAAGCTAGTGAAATGATGAA+AGG | 0.545382 | 8_4:+11189652 | Msa1313300:five_prime_UTR |
| AAAGCTGATGTTTATTCCTT+TGG | 0.259718 | 8_4:+11193526 | Msa1313300:CDS |
| AAATAGTGACCTCCCAGCCT+TGG | 0.459480 | 8_4:-11194086 | None:intergenic |
| AAATTAGGAATGAAGAAGAA+AGG | 0.531049 | 8_4:-11188803 | None:intergenic |
| AACCTATAAATGATCATGTG+AGG | 0.570637 | 8_4:+11189392 | Msa1313300:five_prime_UTR |
| AACCTGCTTCCTATTGGGAT+AGG | 0.611778 | 8_4:+11194844 | Msa1313300:CDS |
| AAGCAAGAGAAAGGTTGTGA+TGG | 0.606710 | 8_4:+11190135 | Msa1313300:CDS |
| AAGCTAGTGAAATGATGAAA+GGG | 0.536539 | 8_4:+11189653 | Msa1313300:five_prime_UTR |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| GAAAACTTTAATGAGAGACA+AGG | - | chr8_4:11188879-11188898 | None:intergenic | 30.0% | |
| TGTATTTGAGAAAACGGTTA+TGG | - | chr8_4:11188934-11188953 | None:intergenic | 30.0% | |
| AACAAAAAGAGGAAAAAAGG+GGG | - | chr8_4:11189147-11189166 | None:intergenic | 30.0% | |
| ATGAAGGTCAAATACATCAT+TGG | - | chr8_4:11189179-11189198 | None:intergenic | 30.0% | |
| AACCTATAAATGATCATGTG+AGG | + | chr8_4:11189392-11189411 | Msa1313300:five_prime_UTR | 30.0% | |
| ACCTATAAATGATCATGTGA+GGG | + | chr8_4:11189393-11189412 | Msa1313300:five_prime_UTR | 30.0% | |
| CTATGCACAAATAGTATCTT+GGG | - | chr8_4:11189459-11189478 | None:intergenic | 30.0% | |
| CTATTTGTGCATAGCAAATT+TGG | + | chr8_4:11189465-11189484 | Msa1313300:five_prime_UTR | 30.0% | |
| ACCTAAAAAAGCAACTTAGA+AGG | - | chr8_4:11189509-11189528 | None:intergenic | 30.0% | |
| AAAGCTAGTGAAATGATGAA+AGG | + | chr8_4:11189652-11189671 | Msa1313300:five_prime_UTR | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_4 | gene | 11188792 | 11195313 | 11188792 | ID=Msa1313300;Name=Msa1313300 |
| chr8_4 | mRNA | 11188792 | 11195313 | 11188792 | ID=Msa1313300-mRNA-1;Parent=Msa1313300;Name=Msa1313300-mRNA-1;_AED=0.07;_eAED=0.07;_QI=628|1|1|1|0.72|0.66|12|402|755 |
| chr8_4 | exon | 11188792 | 11188997 | 11188792 | ID=Msa1313300-mRNA-1:exon:4175;Parent=Msa1313300-mRNA-1 |
| chr8_4 | exon | 11189345 | 11189526 | 11189345 | ID=Msa1313300-mRNA-1:exon:4176;Parent=Msa1313300-mRNA-1 |
| chr8_4 | exon | 11189637 | 11189754 | 11189637 | ID=Msa1313300-mRNA-1:exon:4177;Parent=Msa1313300-mRNA-1 |
| chr8_4 | exon | 11189986 | 11190294 | 11189986 | ID=Msa1313300-mRNA-1:exon:4178;Parent=Msa1313300-mRNA-1 |
| chr8_4 | exon | 11190454 | 11190605 | 11190454 | ID=Msa1313300-mRNA-1:exon:4179;Parent=Msa1313300-mRNA-1 |
| chr8_4 | exon | 11190693 | 11190787 | 11190693 | ID=Msa1313300-mRNA-1:exon:4180;Parent=Msa1313300-mRNA-1 |
| chr8_4 | exon | 11191124 | 11192163 | 11191124 | ID=Msa1313300-mRNA-1:exon:4181;Parent=Msa1313300-mRNA-1 |
| chr8_4 | exon | 11192455 | 11192615 | 11192455 | ID=Msa1313300-mRNA-1:exon:4182;Parent=Msa1313300-mRNA-1 |
| chr8_4 | exon | 11192855 | 11192931 | 11192855 | ID=Msa1313300-mRNA-1:exon:4183;Parent=Msa1313300-mRNA-1 |
| chr8_4 | exon | 11193483 | 11193630 | 11193483 | ID=Msa1313300-mRNA-1:exon:4184;Parent=Msa1313300-mRNA-1 |
| chr8_4 | exon | 11194041 | 11194190 | 11194041 | ID=Msa1313300-mRNA-1:exon:4185;Parent=Msa1313300-mRNA-1 |
| chr8_4 | exon | 11194654 | 11195313 | 11194654 | ID=Msa1313300-mRNA-1:exon:4186;Parent=Msa1313300-mRNA-1 |
| chr8_4 | five_prime_UTR | 11188792 | 11188997 | 11188792 | ID=Msa1313300-mRNA-1:five_prime_utr;Parent=Msa1313300-mRNA-1 |
| chr8_4 | five_prime_UTR | 11189345 | 11189526 | 11189345 | ID=Msa1313300-mRNA-1:five_prime_utr;Parent=Msa1313300-mRNA-1 |
| chr8_4 | five_prime_UTR | 11189637 | 11189754 | 11189637 | ID=Msa1313300-mRNA-1:five_prime_utr;Parent=Msa1313300-mRNA-1 |
| chr8_4 | five_prime_UTR | 11189986 | 11190107 | 11189986 | ID=Msa1313300-mRNA-1:five_prime_utr;Parent=Msa1313300-mRNA-1 |
| chr8_4 | CDS | 11190108 | 11190294 | 11190108 | ID=Msa1313300-mRNA-1:cds;Parent=Msa1313300-mRNA-1 |
| chr8_4 | CDS | 11190454 | 11190605 | 11190454 | ID=Msa1313300-mRNA-1:cds;Parent=Msa1313300-mRNA-1 |
| chr8_4 | CDS | 11190693 | 11190787 | 11190693 | ID=Msa1313300-mRNA-1:cds;Parent=Msa1313300-mRNA-1 |
| chr8_4 | CDS | 11191124 | 11192163 | 11191124 | ID=Msa1313300-mRNA-1:cds;Parent=Msa1313300-mRNA-1 |
| chr8_4 | CDS | 11192455 | 11192615 | 11192455 | ID=Msa1313300-mRNA-1:cds;Parent=Msa1313300-mRNA-1 |
| chr8_4 | CDS | 11192855 | 11192931 | 11192855 | ID=Msa1313300-mRNA-1:cds;Parent=Msa1313300-mRNA-1 |
| chr8_4 | CDS | 11193483 | 11193630 | 11193483 | ID=Msa1313300-mRNA-1:cds;Parent=Msa1313300-mRNA-1 |
| chr8_4 | CDS | 11194041 | 11194190 | 11194041 | ID=Msa1313300-mRNA-1:cds;Parent=Msa1313300-mRNA-1 |
| chr8_4 | CDS | 11194654 | 11194911 | 11194654 | ID=Msa1313300-mRNA-1:cds;Parent=Msa1313300-mRNA-1 |
| chr8_4 | three_prime_UTR | 11194912 | 11195313 | 11194912 | ID=Msa1313300-mRNA-1:three_prime_utr;Parent=Msa1313300-mRNA-1 |
| Gene Sequence |
>Msa1313300 ATGAGTCGAGAGCAGCAAAAGAGGGGGAAGCAAGAGAAAGGTTGTGATGGTGCTGAGAAGGTTATTGTTGCTGTTAAGGCATCGACAAAGGAAATTCCGAAGACAGCTCTTGTTTGGTCACTGACTCATGTTGTTCAACCTGGTGATTGCATTACACTTCTAGTGGTTGTTCCTTCACAAAATTCAGGTAGAAAATGGGGTTTTCCAAGATTTGCTGGTGACTGTGCCGGTGGTATTAAGAAGTATCCTCCAGGAACAATTTTAGAGCAGAAGAGTGATATCACTGATTCTTGCTCTCAGATGATCCTTCAGCTCCATGATGTCTATGATCCAAATAAGATAAATGTCAGGATTAAAATTGTTTCCGGATCACCTTGTGGATCAGTGGCTGCAGAAGCCAAGAAAGGACAAGCTAGTTGGGTTGTATTAGACAAACATCTCAAACCTGAGGAGAAGAAATGCATGGAAGAGCTGCAGTGTAACATTGTTGTTATGAAGCGTGCTCAACCGAAAGTACTTCGCTTGAACTTGATTGGACCAAAAAAGAAGGAAGTTGAAGAAGCATGTTCATCACCTTCTGTGCAAGGCGATATGCTTGAAAAGCAAACCAAAAATAAGATTGATTCTTTAGTAGATTCTATCAAAGGACCAAATGTTACTCCAACTAGCAGCCCTGAGTTAGGTACACCATTCACTACTACTACTGAGGCCGGTACTTCATCAGCTTCAAGCTCTGATCCTGGAACATCACCGTTCTTTATCTCAATGAATGGTGAGTCGAAGAAAGAGGAAACCATCAAGGAAAGTCAAGAACTTTGTGATACTAATTCAGACACAGAAAGTGAAAGTCTGTCCACATCTTCTGCTAGCTTTAGATACCAGCCGTGGATCACGGAATTGCTTTTACATCAACAATCATCTCAACGCAACGAGGAAAGATCAGAGCCATATCACGGTATGCCTCAAGCAACTACAACCAAAGCCTTGCTAGAAAAGTTTTCTAGGCTTGATAGAGGAGCTGAAATTGAAATGTCGGCAGCATATAGAAAGGACTCGGATTTTAGTGGTAATTTAAGAGAAGCAATAGCATTATCAGGAAATGCTCCACTTGGTCCACCTCCATTGTGTTCAATATGTCAACACAAGGCACCTGTATTTGGAAAACCTCCTAGGTGGTTCAGCTATGCTGAGTTGGAACTCGCTACCGGTGGATTTTCACAAGCTAACTTCTTGGCAGAAGGAGGGTATGGTTCTGTTCACCGAGGGGTTCTACCAGAAGGACAGGTCATTGCTGTCAAGCAACATAAATTGGCTAGTTCTCAAGGCGATGTTGAGTTTTGCTCCGAGGTAGAAGTTCTGAGCTGTGCTCAGCACCGAAATGTTGTTATGCTGATTGGATTCTGTATAGAGGATAAGAGAAGGCTACTGGTTTACGAGTACATATGCAATGGATCATTGGATTCACATTTATATGGGCGGCAAAGGGATCCATTAGAATGGTCCGCACGGCAGAAAATTGCTGTTGGAGCTGCTCGTGGGTTAAGGTATCTTCATGAAGAATGCAGAGTAGGTTGTATCATCCACCGTGACATGAGGCCAAACAATATTCTCATAACTCATGATTTTGAACCTCTGGTTGGTGATTTTGGACTGGCAAGGTGGCAGCCTGATGGAGACATGGGTGTGGATACTAGAGTAATTGGAACATTTGGGTATTTGGCTCCTGAATATGCTCAAAGTGGCCAAATCACTGAAAAAGCTGATGTTTATTCCTTTGGGGTGGTACTGGTGGAGCTTGTTACTGGTAGAAAAGCTGTTGATCTCACCAGGCCCAAGGGACAACAGTGCCTTGCTGAGTGGGCACGACCACTATTAGAAGAATATGCTATTGACGAACTGATTGACCCAAGGCTGGGAGGTCACTATTTAGAACATGAGGTCTATTGCATGCTTCATGCTGCTTCATTATGCATAAGAAGAGATCCTCATTCTAGACCACGGATGTCACAGGTTCTCCGAATTCTAGAGGGCGATATGGTGATGGACGCAAATTACATTTCAACTCCTGGCTATGATGTTGGAAACAGGAGTGGTCGCCTTTGGTCAGAGCCACTCCAGAGGCAGCACCATTACAGCGGTCCCCTCTTAGAAGATTCATTAGAGTCATCATTCAGTGGAAATCTATCTCTTGACAAGTACAAACCTGCTTCCTATTGGGATAGGAATAGGGACAAGCCAAGAAGGGCATCATGTGAAGGTGATACTTGA |
| Protein sequence |
>Msa1313300 MSREQQKRGKQEKGCDGAEKVIVAVKASTKEIPKTALVWSLTHVVQPGDCITLLVVVPSQNSGRKWGFPRFAGDCAGGIKKYPPGTILEQKSDITDSCSQMILQLHDVYDPNKINVRIKIVSGSPCGSVAAEAKKGQASWVVLDKHLKPEEKKCMEELQCNIVVMKRAQPKVLRLNLIGPKKKEVEEACSSPSVQGDMLEKQTKNKIDSLVDSIKGPNVTPTSSPELGTPFTTTTEAGTSSASSSDPGTSPFFISMNGESKKEETIKESQELCDTNSDTESESLSTSSASFRYQPWITELLLHQQSSQRNEERSEPYHGMPQATTTKALLEKFSRLDRGAEIEMSAAYRKDSDFSGNLREAIALSGNAPLGPPPLCSICQHKAPVFGKPPRWFSYAELELATGGFSQANFLAEGGYGSVHRGVLPEGQVIAVKQHKLASSQGDVEFCSEVEVLSCAQHRNVVMLIGFCIEDKRRLLVYEYICNGSLDSHLYGRQRDPLEWSARQKIAVGAARGLRYLHEECRVGCIIHRDMRPNNILITHDFEPLVGDFGLARWQPDGDMGVDTRVIGTFGYLAPEYAQSGQITEKADVYSFGVVLVELVTGRKAVDLTRPKGQQCLAEWARPLLEEYAIDELIDPRLGGHYLEHEVYCMLHAASLCIRRDPHSRPRMSQVLRILEGDMVMDANYISTPGYDVGNRSGRLWSEPLQRQHHYSGPLLEDSLESSFSGNLSLDKYKPASYWDRNRDKPRRASCEGDT* |
