AlfalfaGEDB | Msa0205820

M. sativa cultivar zhongmu-4 / Msa0205820

KEGG: K17604
GO: -
PFAM: PF03101.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0205820	XP_042969196.1	40.399	552	240	10	32	501	7	551	5.69e-119	376

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0205820	sp\|Q9SWG3\|FAR1_ARATH	35.829	187	108	1	213	387	368	554	2.01e-28	124
Msa0205820	sp\|Q9SWG3\|FAR1_ARATH	26.316	247	144	8	39	272	48	269	3.67e-15	82.8

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0205820	A0A287QZU0	52.802	339	156	3	20	357	34	369	2.42e-121	373

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TFs/TRs:

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Gene ID	Type	Classification
Msa0205820	TF	FAR1

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0205820	Msa1207930	0.802059	6.715096e-49	-8.615850e-47
Msa0205820	Msa1207940	0.858973	5.701813e-63	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0205820	MtrunA17_Chr1g0196541	43.925	428	138	9	232	592	339	731	2.53e-94	305
Msa0205820	MtrunA17_Chr1g0196541	56.284	183	80	0	48	230	3	185	5.56e-63	221
Msa0205820	MtrunA17_Chr2g0296781	75.000	184	46	0	48	231	3	186	2.24e-92	300
Msa0205820	MtrunA17_Chr2g0296781	78.916	166	35	0	229	394	336	501	8.65e-87	285
Msa0205820	MtrunA17_Chr2g0296781	54.861	144	52	5	399	530	573	715	1.12e-25	112
Msa0205820	MtrunA17_Chr6g0477261	81.928	166	30	0	229	394	225	390	1.13e-90	293
Msa0205820	MtrunA17_Chr6g0477261	89.333	75	8	0	157	231	1	75	5.37e-37	146
Msa0205820	MtrunA17_Chr6g0477261	45.161	186	79	5	399	562	462	646	1.73e-31	129
Msa0205820	MtrunA17_Chr5g0441581	49.123	171	83	1	232	398	367	537	1.12e-45	172
Msa0205820	MtrunA17_Chr5g0441581	31.801	261	145	7	18	272	5	238	2.70e-27	117

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0205820	AT4G15090.1	35.829	187	108	1	213	387	368	554	2.05e-29	124
Msa0205820	AT4G15090.1	26.316	247	144	8	39	272	48	269	3.73e-16	82.8
Msa0205820	AT4G15090.5	35.829	187	108	1	213	387	368	554	2.05e-29	124
Msa0205820	AT4G15090.5	26.316	247	144	8	39	272	48	269	3.73e-16	82.8
Msa0205820	AT4G15090.3	35.829	187	108	1	213	387	368	554	2.05e-29	124
Msa0205820	AT4G15090.3	26.316	247	144	8	39	272	48	269	3.73e-16	82.8
Msa0205820	AT4G15090.2	35.829	187	108	1	213	387	368	554	2.05e-29	124
Msa0205820	AT4G15090.2	26.316	247	144	8	39	272	48	269	3.73e-16	82.8
Msa0205820	AT4G15090.4	35.829	187	108	1	213	387	368	554	2.05e-29	124
Msa0205820	AT4G15090.4	26.316	247	144	8	39	272	48	269	3.73e-16	82.8

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Find 73 sgRNAs with CRISPR-Local

Find 172 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATTCAAAGAATTTCAAG+TGG	0.644675	2_1:-33082641	Msa0205820:CDS
AAAGTGAGGAGACTACGACT+TGG	0.649574	2_1:-33083540	Msa0205820:CDS
AACCTTCTTCCACAATGACA+GGG	0.618602	2_1:+33082148	None:intergenic
AACGTGTCTCTCACATATGC+AGG	0.530851	2_1:+33082892	None:intergenic
AACTCCAAAACCCATAGATT+GGG	0.455626	2_1:+33083956	None:intergenic
AAGAGTTATGCCCAATCTAT+GGG	0.502102	2_1:-33083966	Msa0205820:CDS
AAGGAAAACAAGGCTGATTT+TGG	0.221363	2_1:-33082739	Msa0205820:CDS
AAGGTCGTCCTCCTTCAAAA+AGG	0.483624	2_1:-33082074	Msa0205820:CDS
AAGTATTGAATTTGGATTGA+AGG	0.452798	2_1:+33081665	None:intergenic
AAGTGAGGAGACTACGACTT+GGG	0.414907	2_1:-33083539	Msa0205820:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATTTGTATCTTCTGATGAAG+AGG	-	chr2_1:33081613-33081632	Msa0205820:intron	30.0%
TTTAACAAAAACCAAGTGCA+AGG	-	chr2_1:33081898-33081917	Msa0205820:intron	30.0%
TTAGAACTTAATGATCAAGC+TGG	-	chr2_1:33082061-33082080	Msa0205820:CDS	30.0%
TTACTTGGTTATTCCAAACA+TGG	-	chr2_1:33082494-33082513	Msa0205820:intron	30.0%
TGATCAGTAATTATGGCATT+CGG	+	chr2_1:33082544-33082563	None:intergenic	30.0%
TCTGTCTTGATCAGTAATTA+TGG	+	chr2_1:33082551-33082570	None:intergenic	30.0%
TTTGAGTAAGAGTGATTTCA+TGG	-	chr2_1:33082720-33082739	Msa0205820:CDS	30.0%
CTGTTAACTTAAGAACACAA+AGG	+	chr2_1:33083305-33083324	None:intergenic	30.0%
CTTTGTGTTCTTAAGTTAAC+AGG	-	chr2_1:33083303-33083322	Msa0205820:intron	30.0%
TGAGAGAAAATATAAGAGGA+TGG	+	chr2_1:33083345-33083364	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_1	gene	33081362	33084381	33081362	ID=Msa0205820;Name=Msa0205820
chr2_1	mRNA	33081362	33084381	33081362	ID=Msa0205820-mRNA-1;Parent=Msa0205820;Name=Msa0205820-mRNA-1;_AED=0.41;_eAED=0.41;_QI=0\|0.42\|0.12\|1\|1\|1\|8\|0\|599
chr2_1	exon	33084360	33084381	33084360	ID=Msa0205820-mRNA-1:exon:11472;Parent=Msa0205820-mRNA-1
chr2_1	exon	33083470	33084140	33083470	ID=Msa0205820-mRNA-1:exon:11471;Parent=Msa0205820-mRNA-1
chr2_1	exon	33082522	33083013	33082522	ID=Msa0205820-mRNA-1:exon:11470;Parent=Msa0205820-mRNA-1
chr2_1	exon	33081987	33082319	33081987	ID=Msa0205820-mRNA-1:exon:11469;Parent=Msa0205820-mRNA-1
chr2_1	exon	33081844	33081915	33081844	ID=Msa0205820-mRNA-1:exon:11468;Parent=Msa0205820-mRNA-1
chr2_1	exon	33081647	33081742	33081647	ID=Msa0205820-mRNA-1:exon:11467;Parent=Msa0205820-mRNA-1
chr2_1	exon	33081508	33081564	33081508	ID=Msa0205820-mRNA-1:exon:11466;Parent=Msa0205820-mRNA-1
chr2_1	exon	33081362	33081418	33081362	ID=Msa0205820-mRNA-1:exon:11465;Parent=Msa0205820-mRNA-1
chr2_1	CDS	33084360	33084381	33084360	ID=Msa0205820-mRNA-1:cds;Parent=Msa0205820-mRNA-1
chr2_1	CDS	33083470	33084140	33083470	ID=Msa0205820-mRNA-1:cds;Parent=Msa0205820-mRNA-1
chr2_1	CDS	33082522	33083013	33082522	ID=Msa0205820-mRNA-1:cds;Parent=Msa0205820-mRNA-1
chr2_1	CDS	33081987	33082319	33081987	ID=Msa0205820-mRNA-1:cds;Parent=Msa0205820-mRNA-1
chr2_1	CDS	33081844	33081915	33081844	ID=Msa0205820-mRNA-1:cds;Parent=Msa0205820-mRNA-1
chr2_1	CDS	33081647	33081742	33081647	ID=Msa0205820-mRNA-1:cds;Parent=Msa0205820-mRNA-1
chr2_1	CDS	33081508	33081564	33081508	ID=Msa0205820-mRNA-1:cds;Parent=Msa0205820-mRNA-1
chr2_1	CDS	33081362	33081418	33081362	ID=Msa0205820-mRNA-1:cds;Parent=Msa0205820-mRNA-1

Gene Sequence

>Msa0205820

ATGGAAACAGTGCAAAAATCAATGGAAGAAGTATTTGTATCTTCTGATGAAGAGGATATCAGAGAAGGAAATAATAATTTTGATGATAGTTTAGAAGAAGATATTGTTCATGAAGATGAAAAACCTCAGGTTGGAACTATATTTAGCTCTAAAGATGAACTTCTAGAGTATTACAAGAGTTATGCCCAATCTATGGGTTTTGGAGTTACTAAACTTAGTAACAAAAATACAGATGATGGGAAAAAATACTTTACTCTAGCATGTAGTCGTGGATCAAAGTATGTGAGTAAGGCGAAGAAGCTTTTGAACCCAAATCCTTTAACAAAAACCAAGTGCAAGGCTAGATTGAATGCATGTATTTCTTTAGATGGAACAGTTACTATTACAAAACTTTTTCTTGAGCATAATCATGATCTAATCCCTGACAAAGCACGCTATTTTAGAAGTAGTAAGTTTTTGGATCCTCGTTTGAAAAGGAGGTTAGAACTTAATGATCAAGCTGGAATAAATGTAAGCAGAAATTTTCGATCAATGGTTGTTGAAGCAAATGGCTATGAGAATATGACATTTGGTGAAAAGGACTGTAGAAACTATATAGATAAAGTGAGGAGACTACGACTTGGGGCTGGAGATGCTGAAGCTATTCAAAATTATTTCGTTAAAATGCAAAAGCAAAATAGTCAGTTCTATTATAGTGATTTCATGGAAAAGTGGGAAAGAATGATTGAAGATTTTGAACTTCATGATAATGAATGGTTGAAGGGGTTATTTGATGAAAGAAATCGTTGGGTTCCTGCATATGTGAGAGACACGTTTTGGGCGGGAATGTCAACGACTCAAAGAAGTGAAAGTATGAACTCGTTTTTTGATGGATATGTAAATTCAAAGACAGCATTGAAGCAATTTGTTGAACAATATGACAATGCATTGAGAGATAAAGTTGAAAAGGAAAACAAGGCTGATTTTGGTTCATTTAATACAGTGATAGCTTGTATCAGTCATTTTGGCTTTGAGTTGCAATTCCAGAAAGCATTCACCAATGCAAAATTCAAAGAATTTCAAGTGGAGGTTGCTTCTATGATTTACTGTCATACTTGCTTTGAGAGATTGGAAGGATCAAATTCTATATATTCTGTTATAGAGAGCAAGAAAAACTTTGACAAAATTAAAGATATAGTGTTTGAGTTGGAAAATGCTAAATTACAAAGAGTTGATAAAGCATGTGCTGACTTTTATGAAGTTGCTTCTGAGGTGATTAATACCGATGATGATTTGTTTAAAATGAGGAATTGGATCAAGGAAGTAAAAAATGAGTTTACCTCTAAAGAAGCATCCCCTGTCATTGTGGAAGAAGGTTCGGTTCAAAATCAAGTGATTAAAATTCTTGACCCTATACCGACTCGAAGTAAAGGTCGTCCTCCTTCAAAAAGGAAGTCTTCTAAAGTTGATCAAATTGTGAAGAAAATTTCAAAAAAGAAGACCCAAAAAAGAAGTCAACAGAGTGGAAATCAACAAGAGGGCCTGTGTGCGTCTAAAGTACAAGAGAGTGTTCAAGTAGTTGATGTAGTTGGCACACAAGAGAGTGTTCAAGGGGAACATATTAGTTTTGGGAGGAATGGACATGGTGGTGTAAATAATTTAGCTCCCTTCAATCCAAATTCAATACTTATGAGTGAAGTAAATCAGGTTCCATTTTATTCTCAAGTCATGAATCATGGTGTTTCCTATTTTGAATTGTTACAGGCACAACACCATGTGAATGATCCGTTGTCGTCATCAATAACACGTGAATCATTTTGA

Protein sequence

>Msa0205820

METVQKSMEEVFVSSDEEDIREGNNNFDDSLEEDIVHEDEKPQVGTIFSSKDELLEYYKSYAQSMGFGVTKLSNKNTDDGKKYFTLACSRGSKYVSKAKKLLNPNPLTKTKCKARLNACISLDGTVTITKLFLEHNHDLIPDKARYFRSSKFLDPRLKRRLELNDQAGINVSRNFRSMVVEANGYENMTFGEKDCRNYIDKVRRLRLGAGDAEAIQNYFVKMQKQNSQFYYSDFMEKWERMIEDFELHDNEWLKGLFDERNRWVPAYVRDTFWAGMSTTQRSESMNSFFDGYVNSKTALKQFVEQYDNALRDKVEKENKADFGSFNTVIACISHFGFELQFQKAFTNAKFKEFQVEVASMIYCHTCFERLEGSNSIYSVIESKKNFDKIKDIVFELENAKLQRVDKACADFYEVASEVINTDDDLFKMRNWIKEVKNEFTSKEASPVIVEEGSVQNQVIKILDPIPTRSKGRPPSKRKSSKVDQIVKKISKKKTQKRSQQSGNQQEGLCASKVQESVQVVDVVGTQESVQGEHISFGRNGHGGVNNLAPFNPNSILMSEVNQVPFYSQVMNHGVSYFELLQAQHHVNDPLSSSITRESF*