AlfalfaGEDB | Msa0207070

M. sativa cultivar zhongmu-4 / Msa0207070

KEGG: K20793
GO: -
PFAM: PF00583.28

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0207070	XP_013463544.1	94.684	301	13	1	7	307	1	298	0.0	592

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0207070	A0A072V684	94.684	301	13	1	7	307	1	298	0.0	592

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TFs/TRs:

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Gene ID	Type	Classification
Msa0207070	TR	GNAT

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0207070	Msa0246190	0.834356	3.142293e-56	-8.615850e-47
Msa0207070	Msa0284690	0.951331	2.559756e-109	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0207070	MtrunA17_Chr2g0300621	94.684	301	13	1	7	307	1	298	0.0	592
Msa0207070	MtrunA17_Chr4g0073171	28.723	188	109	5	115	286	58	236	7.42e-12	64.7

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0207070	AT2G06025.1	56.522	299	116	3	7	305	1	285	3.92e-117	339
Msa0207070	AT2G06025.6	56.522	299	116	3	7	305	1	285	3.92e-117	339
Msa0207070	AT2G06025.4	56.522	299	116	3	7	305	1	285	3.92e-117	339
Msa0207070	AT2G06025.3	56.641	256	97	3	7	262	1	242	5.91e-99	291
Msa0207070	AT2G06025.5	56.641	256	97	3	7	262	1	242	5.91e-99	291
Msa0207070	AT2G06025.2	56.641	256	97	3	7	262	1	242	5.91e-99	291
Msa0207070	AT2G06025.7	54.054	222	88	3	7	228	1	208	8.84e-79	239
Msa0207070	AT4G28030.1	30.097	206	102	7	111	292	58	245	1.11e-12	67.4

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Find 92 sgRNAs with CRISPR-Local

Find 192 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATCTTACATAGTCCATG+AGG	0.666948	2_1:+37484827	None:intergenic
AAACCTTCTTCCACCACTTT+AGG	0.251194	2_1:+37484568	None:intergenic
AAACTCTCGGTTGTTACCAG+AGG	0.674396	2_1:-37482121	Msa0207070:CDS
AAATTTATGTGTTGCCAAAT+TGG	0.283413	2_1:-37482461	Msa0207070:CDS
AAGCCCAATTGTTCCTAAAG+TGG	0.498436	2_1:-37484581	Msa0207070:CDS
AAGCGGTGCAAGGTGCAAAA+TGG	0.311231	2_1:-37482948	Msa0207070:CDS
AATATCCGATATTTGCTGCA+AGG	0.586800	2_1:-37482656	Msa0207070:CDS
AATGAAACAGAACAAGAACA+GGG	0.696176	2_1:-37481828	Msa0207070:three_prime_UTR
AATTTGCAACTGTTCCAGAT+TGG	0.457980	2_1:+37482044	None:intergenic
ACAGTTATTGTACCAAAAGA+TGG	0.467482	2_1:-37482264	Msa0207070:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACATATTATCCATTCCATTG+TGG	-	chr2_1:37481735-37481754	Msa0207070:three_prime_UTR	30.0%
TGAATTGGAATAGTTGACAT+AGG	+	chr2_1:37481969-37481988	None:intergenic	30.0%
AAAATCTTACATAGTCCATG+AGG	+	chr2_1:37482063-37482082	None:intergenic	30.0%
GTTTGATATGAATAGGTCTA+TGG	-	chr2_1:37482201-37482220	Msa0207070:intron	30.0%
TAAGAATAAGAAGATGAAGG+AGG	-	chr2_1:37482264-37482283	Msa0207070:CDS	30.0%
CCATACAATTTCATACACTA+AGG	+	chr2_1:37482714-37482733	None:intergenic	30.0%
CCTTAGTGTATGAAATTGTA+TGG	-	chr2_1:37482711-37482730	Msa0207070:CDS	30.0%
TGAAGGGACTATAATTCAAA+GGG	+	chr2_1:37483359-37483378	None:intergenic	30.0%
ATGAAGGGACTATAATTCAA+AGG	+	chr2_1:37483360-37483379	None:intergenic	30.0%
GATTATAAACCAGATCAAGA+TGG	-	chr2_1:37483624-37483643	Msa0207070:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_1	gene	37481733	37485176	37481733	ID=Msa0207070;Name=Msa0207070
chr2_1	mRNA	37481733	37485176	37481733	ID=Msa0207070-mRNA-1;Parent=Msa0207070;Name=Msa0207070-mRNA-1;_AED=0.10;_eAED=0.10;_QI=218\|1\|1\|1\|0.87\|0.77\|9\|359\|308
chr2_1	exon	37481733	37482157	37481733	ID=Msa0207070-mRNA-1:exon:12130;Parent=Msa0207070-mRNA-1
chr2_1	exon	37482256	37482332	37482256	ID=Msa0207070-mRNA-1:exon:12129;Parent=Msa0207070-mRNA-1
chr2_1	exon	37482398	37482551	37482398	ID=Msa0207070-mRNA-1:exon:12128;Parent=Msa0207070-mRNA-1
chr2_1	exon	37482640	37482744	37482640	ID=Msa0207070-mRNA-1:exon:12127;Parent=Msa0207070-mRNA-1
chr2_1	exon	37482923	37482988	37482923	ID=Msa0207070-mRNA-1:exon:12126;Parent=Msa0207070-mRNA-1
chr2_1	exon	37483079	37483115	37483079	ID=Msa0207070-mRNA-1:exon:12125;Parent=Msa0207070-mRNA-1
chr2_1	exon	37483632	37483684	37483632	ID=Msa0207070-mRNA-1:exon:12124;Parent=Msa0207070-mRNA-1
chr2_1	exon	37484447	37484693	37484447	ID=Msa0207070-mRNA-1:exon:12123;Parent=Msa0207070-mRNA-1
chr2_1	exon	37484837	37485176	37484837	ID=Msa0207070-mRNA-1:exon:12122;Parent=Msa0207070-mRNA-1
chr2_1	five_prime_UTR	37484959	37485176	37484959	ID=Msa0207070-mRNA-1:five_prime_utr;Parent=Msa0207070-mRNA-1
chr2_1	CDS	37484837	37484958	37484837	ID=Msa0207070-mRNA-1:cds;Parent=Msa0207070-mRNA-1
chr2_1	CDS	37484447	37484693	37484447	ID=Msa0207070-mRNA-1:cds;Parent=Msa0207070-mRNA-1
chr2_1	CDS	37483632	37483684	37483632	ID=Msa0207070-mRNA-1:cds;Parent=Msa0207070-mRNA-1
chr2_1	CDS	37483079	37483115	37483079	ID=Msa0207070-mRNA-1:cds;Parent=Msa0207070-mRNA-1
chr2_1	CDS	37482923	37482988	37482923	ID=Msa0207070-mRNA-1:cds;Parent=Msa0207070-mRNA-1
chr2_1	CDS	37482640	37482744	37482640	ID=Msa0207070-mRNA-1:cds;Parent=Msa0207070-mRNA-1
chr2_1	CDS	37482398	37482551	37482398	ID=Msa0207070-mRNA-1:cds;Parent=Msa0207070-mRNA-1
chr2_1	CDS	37482256	37482332	37482256	ID=Msa0207070-mRNA-1:cds;Parent=Msa0207070-mRNA-1
chr2_1	CDS	37482092	37482157	37482092	ID=Msa0207070-mRNA-1:cds;Parent=Msa0207070-mRNA-1
chr2_1	three_prime_UTR	37481733	37482091	37481733	ID=Msa0207070-mRNA-1:three_prime_utr;Parent=Msa0207070-mRNA-1

Gene Sequence

>Msa0207070

ATGAAAATCATGTCACCTATGTCAACTATTCCAATTCATAAACCTGAATTTCATATCTTACTTTATAATTCATCTCCAACCCATAATTACAAATGCACCAAAATTACATCCTCATGGACTATGTCTATGGATTCTAAATTTTCTTCAACAATGAATAGTAATAATGATATTAAGAATAAGAAGATGAAGGAGGAACTATCTGTGAAGCTTTCAAGCCCAATTGTTCCTAAAGTGGTGGAAGAAGGTTTGAGCTCGAATGGTCTTCGATTTGATCGGTTGCAGCCGTCGGAACAAGAGTTGATTAGAGAAAATAGATTTGAGTTTGGGCAATTTGTAGCTAGGGAAGCTGTGCTTGATGAAGAGTTTTGGACAGCAGCCTGGCTAAGGGCAGAAAGTCATTGGGAGAATCAAACATATGAAAGATATATTGATATCTACAAAAAGAAATTTGCTGATCAGGAATTTAATGCGGTAAAAAAGCGGTGCAAGGTGCAAAATGGGGACAGCTGCGCATGCATTATCGCGGTGAGAAAGGAGCAGAAGAATGTAAAGCGCTCGATAATAAAAAGTGTTGTAGGAACACTTGACTTGAATATCCGATATTTGCTGCAAGGAGAGACTTTTCCTGGGGAACGAGACAAAACTCCTTTTTGTTGCAGCATAAACAGAACACCACCAAGTAGATATGGTTACATTGCAAATTTATGTGTTGCCAAATTGGCTCGTCGACAAGGAATTGCAAGCAGCATGATGTCTTTTGCTGTTGAAAATGCAAAATCTAATGGTGTGAGTCAGGTATATGTGCATGTGGACAGAAATAATAGGCCGGGACAGTTATTGTACCAAAAGATGGGCTTTGAGATGGTTGAAACTGCAAACTCTCGGTTGTTACCAGAGGAAACATACTTGCTACGTTTACAGACGTAA

Protein sequence

>Msa0207070

MKIMSPMSTIPIHKPEFHILLYNSSPTHNYKCTKITSSWTMSMDSKFSSTMNSNNDIKNKKMKEELSVKLSSPIVPKVVEEGLSSNGLRFDRLQPSEQELIRENRFEFGQFVAREAVLDEEFWTAAWLRAESHWENQTYERYIDIYKKKFADQEFNAVKKRCKVQNGDSCACIIAVRKEQKNVKRSIIKSVVGTLDLNIRYLLQGETFPGERDKTPFCCSINRTPPSRYGYIANLCVAKLARRQGIASSMMSFAVENAKSNGVSQVYVHVDRNNRPGQLLYQKMGFEMVETANSRLLPEETYLLRLQT*