AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0213130

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0213130 MtrunA17_Chr2g0312781 88.940 217 24 0 1 217 18 234 1.20e-145 405
Msa0213130 MtrunA17_Chr2g0312771 89.252 214 23 0 1 214 1 214 4.98e-145 403
Msa0213130 MtrunA17_Chr2g0312751 87.850 214 26 0 1 214 1 214 2.42e-143 399
Msa0213130 MtrunA17_Chr2g0312731 80.093 216 43 0 1 216 1 216 8.29e-134 374
Msa0213130 MtrunA17_Chr2g0312811 76.852 216 48 1 1 214 1 216 2.74e-125 353
Msa0213130 MtrunA17_Chr2g0312791 72.146 219 58 3 1 216 1 219 4.39e-116 330
Msa0213130 MtrunA17_Chr2g0312681 64.651 215 76 0 1 215 1 215 3.25e-110 315
Msa0213130 MtrunA17_Chr2g0312691 64.651 215 76 0 1 215 1 215 4.89e-107 306
Msa0213130 MtrunA17_Chr8g0357241 65.421 214 74 0 1 214 1 214 2.36e-102 295
Msa0213130 MtrunA17_Chr8g0357211 64.319 213 76 0 1 213 1 213 6.81e-101 291
Msa0213130 MtrunA17_Chr3g0107731 62.381 210 79 0 5 214 10 219 8.63e-101 291
Msa0213130 MtrunA17_Chr2g0312711 62.326 215 80 1 1 215 1 214 1.80e-100 290
Msa0213130 MtrunA17_Chr3g0107741 60.377 212 84 0 3 214 8 219 6.81e-97 281
Msa0213130 MtrunA17_Chr2g0312721 60.094 213 85 0 1 213 1 213 6.50e-93 271
Msa0213130 MtrunA17_Chr5g0433801 53.271 214 98 2 2 214 4 216 2.53e-81 241
Msa0213130 MtrunA17_Chr4g0069471 60.656 183 69 2 1 182 22 202 7.09e-79 235
Msa0213130 MtrunA17_Chr8g0379581 41.748 206 117 2 2 205 3 207 3.28e-51 165
Msa0213130 MtrunA17_Chr1g0196661 43.689 206 111 4 4 205 3 207 3.65e-51 165
Msa0213130 MtrunA17_Chr5g0415411 42.723 213 113 5 2 205 3 215 3.71e-50 162
Msa0213130 MtrunA17_Chr3g0132581 42.927 205 114 3 3 205 4 207 3.02e-49 160
Msa0213130 MtrunA17_Chr8g0379561 42.157 204 115 2 4 205 6 208 3.42e-49 160
Msa0213130 MtrunA17_Chr7g0240681 40.488 205 116 4 3 203 6 208 4.39e-49 160
Msa0213130 MtrunA17_Chr7g0240691 42.439 205 112 4 3 203 36 238 9.81e-49 160
Msa0213130 MtrunA17_Chr4g0008781 41.827 208 112 6 3 205 7 210 1.03e-48 159
Msa0213130 MtrunA17_Chr5g0417011 36.493 211 127 2 2 205 5 215 2.25e-48 158
Msa0213130 MtrunA17_Chr7g0240611 40.976 205 112 5 3 204 6 204 6.25e-48 157
Msa0213130 MtrunA17_Chr7g0240641 40.686 204 116 4 3 203 6 207 7.82e-48 156
Msa0213130 MtrunA17_Chr4g0029341 41.905 210 115 4 1 205 1 208 1.18e-47 156
Msa0213130 MtrunA17_Chr7g0240631 39.216 204 119 4 3 203 6 207 7.27e-47 154
Msa0213130 MtrunA17_Chr4g0008791 40.887 203 115 4 4 203 7 207 4.06e-46 152
Msa0213130 MtrunA17_Chr1g0196691 39.130 207 118 5 4 204 28 232 1.51e-45 151
Msa0213130 MtrunA17_Chr1g0196721 42.211 199 110 3 11 205 9 206 6.03e-45 149
Msa0213130 MtrunA17_Chr7g0240671 39.512 205 119 5 1 204 1 201 7.58e-45 149
Msa0213130 MtrunA17_Chr6g0483141 39.474 190 112 2 18 205 17 205 1.74e-44 148
Msa0213130 MtrunA17_Chr1g0196771 38.889 216 122 6 3 214 2 211 2.18e-44 147
Msa0213130 MtrunA17_Chr7g0240531 40.291 206 118 5 3 205 6 209 2.73e-44 147
Msa0213130 MtrunA17_Chr7g0240561 37.255 204 123 4 3 203 6 207 1.99e-43 145
Msa0213130 MtrunA17_Chr7g0240541 39.216 204 119 5 3 203 6 207 3.28e-43 144
Msa0213130 MtrunA17_Chr7g0240551 39.806 206 119 5 3 205 6 209 4.02e-43 144
Msa0213130 MtrunA17_Chr7g0240661 40.476 210 120 5 1 209 4 209 5.16e-43 144
Msa0213130 MtrunA17_Chr8g0363751 35.484 217 125 4 4 208 5 218 1.03e-42 144
Msa0213130 MtrunA17_Chr7g0240651 40.000 205 114 5 3 204 6 204 5.86e-42 141
Msa0213130 MtrunA17_Chr7g0240581 37.745 204 122 4 3 203 6 207 6.17e-42 141
Msa0213130 MtrunA17_Chr1g0196711 39.535 215 122 5 4 211 3 216 5.97e-41 139
Msa0213130 MtrunA17_Chr7g0240511 39.409 203 115 5 3 203 6 202 9.37e-41 138
Msa0213130 MtrunA17_Chr1g0212301 37.500 224 128 6 1 217 1 219 2.62e-38 132
Msa0213130 MtrunA17_Chr7g0240521 38.308 201 119 5 3 200 13 211 4.54e-36 126
Msa0213130 MtrunA17_Chr8g0391631 39.241 158 83 3 4 151 21 175 1.27e-35 124
Msa0213130 MtrunA17_Chr2g0313561 63.235 68 25 0 47 114 1 68 8.37e-30 105
Msa0213130 MtrunA17_Chr4g0076691 31.579 171 98 5 4 159 15 181 3.33e-14 68.9
Msa0213130 MtrunA17_Chr1g0181861 28.641 206 132 7 10 209 33 229 1.45e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0213130 AT1G78380.1 68.224 214 68 0 1 214 1 214 2.76e-112 320
Msa0213130 AT1G17180.1 66.355 214 72 0 1 214 1 214 2.95e-111 318
Msa0213130 AT1G78370.1 64.815 216 76 0 1 216 1 216 4.39e-107 307
Msa0213130 AT1G78340.1 64.019 214 77 0 1 214 1 214 4.19e-105 302
Msa0213130 AT1G17170.1 64.486 214 75 1 1 214 1 213 1.88e-104 300
Msa0213130 AT1G78320.1 64.019 214 77 0 1 214 1 214 2.48e-104 300
Msa0213130 AT1G78360.1 64.186 215 76 1 1 214 1 215 1.01e-103 299
Msa0213130 AT1G53680.1 58.962 212 85 2 5 214 8 219 2.31e-94 275
Msa0213130 AT1G17190.1 57.075 212 91 0 3 214 4 215 4.00e-93 272
Msa0213130 AT3G43800.1 55.300 217 93 3 3 215 4 220 3.43e-84 249
Msa0213130 AT1G78320.2 55.607 214 68 1 1 214 1 187 1.23e-82 244
Msa0213130 AT2G29420.1 43.902 205 112 2 2 204 7 210 1.12e-58 184
Msa0213130 AT3G09270.1 41.748 206 117 2 3 205 5 210 2.94e-52 168
Msa0213130 AT2G29440.1 44.660 206 108 4 3 204 5 208 2.15e-51 166
Msa0213130 AT2G29490.1 39.171 217 126 4 3 215 6 220 2.25e-50 163
Msa0213130 AT2G29450.1 43.961 207 109 5 3 204 5 209 3.02e-50 163
Msa0213130 AT1G10360.1 40.183 219 123 4 2 212 3 221 3.69e-49 160
Msa0213130 AT2G29480.1 40.777 206 116 4 3 204 6 209 8.29e-49 159
Msa0213130 AT2G29470.1 40.488 205 115 5 5 204 8 210 1.70e-47 156
Msa0213130 AT1G59700.1 41.096 219 120 5 3 212 5 223 3.90e-47 155
Msa0213130 AT2G29460.1 39.806 206 118 4 3 204 6 209 1.24e-46 154
Msa0213130 AT1G10370.1 37.838 222 116 4 2 208 3 217 7.60e-46 152
Msa0213130 AT1G27140.1 37.333 225 131 4 3 217 5 229 2.97e-44 148
Msa0213130 AT1G59670.1 40.909 220 119 5 3 212 5 223 3.74e-44 147
Msa0213130 AT1G74590.1 35.484 217 134 3 4 214 7 223 1.98e-43 146
Msa0213130 AT1G69930.1 38.571 210 118 4 5 205 13 220 6.43e-43 144
Msa0213130 AT1G27130.1 36.792 212 125 4 3 205 5 216 1.59e-42 143
Msa0213130 AT5G62480.1 34.802 227 136 4 1 216 5 230 4.68e-42 142
Msa0213130 AT1G69920.1 37.019 208 116 5 5 200 35 239 2.81e-39 135
Msa0213130 AT5G62480.2 28.889 225 122 5 3 216 7 204 1.88e-25 99.0
Msa0213130 AT5G62480.3 28.889 225 121 5 3 216 7 203 2.04e-25 99.0

Find 79 sgRNAs with CRISPR-Local

Find 186 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGGGAGACCGGACTTATTT+TGG 0.184590 2_1:-55704583 Msa0213130:CDS
TTGGCCCAAGCAATGATCTT+AGG 0.247229 2_1:+55704460 None:intergenic
GACATCTATGAATTCTTTCT+TGG 0.270245 2_1:+55704627 None:intergenic
GCAACTAAAGATTCACAAAT+AGG 0.275943 2_1:+55705044 None:intergenic
TATTATATTATGTAATAAAA+AGG 0.285708 2_1:-55704167 Msa0213130:three_prime_UTR
CAAATTGTTGGAGCAAGAAT+TGG 0.293479 2_1:-55704602 Msa0213130:CDS
TTTATTTATAGAGTGGGATT+TGG 0.307528 2_1:+55705347 None:intergenic
AAGTGATTCTGCTTGATTCC+TGG 0.317076 2_1:-55705220 Msa0213130:CDS
TTCAGTATATTGATGAGGTT+TGG 0.327749 2_1:-55705019 Msa0213130:CDS
TAAATCATTTATGACGTGTT+TGG 0.334319 2_1:+55704217 None:intergenic
TATGATGGTTTATTGTGATT+TGG 0.340334 2_1:-55704254 Msa0213130:three_prime_UTR
AAATCCCACTCTATAAATAA+AGG 0.357835 2_1:-55705345 Msa0213130:five_prime_UTR
AAGGAACCTTTGGGCCAAAA+AGG 0.360471 2_1:-55704668 Msa0213130:CDS
GATTCCTGGGCAAGTCCATT+TGG 0.366880 2_1:-55705206 Msa0213130:CDS
CTTACAAAATGCACAACGTT+TGG 0.369957 2_1:+55704132 None:intergenic
GTTTAGATGAACGGTGAGAT+TGG 0.375881 2_1:-55704319 Msa0213130:three_prime_UTR
CATAGATGTCCTCAAATTGT+TGG 0.381980 2_1:-55704614 Msa0213130:CDS
CAGAATAGCCCTAGCTGAAA+AGG 0.384656 2_1:-55705174 Msa0213130:CDS
ATGAAGGTGGAAGGAACCTT+TGG 0.390594 2_1:-55704678 Msa0213130:CDS
CACTTATTCCATTCTACACT+TGG 0.395366 2_1:-55704531 Msa0213130:CDS
ATTCTTGCTCCAACAATTTG+AGG 0.395572 2_1:+55704605 None:intergenic
TTTAAAAGCTATGAGACCTT+TGG 0.396998 2_1:-55704508 Msa0213130:CDS
TCATGGACCTTATCCTGATC+AGG 0.401755 2_1:+55704400 None:intergenic
AGACGGGTTATTGAGTAGGT+TGG 0.414239 2_1:-55704357 Msa0213130:exon
TTACCGTTATGAATCAGAAC+AGG 0.415381 2_1:+55705068 None:intergenic
AGAGATCACAAGCTAGGTTC+TGG 0.428783 2_1:-55704959 Msa0213130:CDS
ATAGAATAGATAGATTGTTA+TGG 0.430247 2_1:+55705285 None:intergenic
TATGTTGAATTGCACAATAT+AGG 0.449373 2_1:-55705458 Msa0213130:five_prime_UTR
TAGAACCTTTATTTATAGAG+TGG 0.450475 2_1:+55705340 None:intergenic
TTCAAAGTCACTCCCTGATC+AGG 0.452471 2_1:-55704413 Msa0213130:CDS
CTCAATCTGCATACATCTCT+TGG 0.460115 2_1:+55704441 None:intergenic
TGGCCCAAGCAATGATCTTA+GGG 0.464217 2_1:+55704461 None:intergenic
CTAGCTTGTGATCTCTGATA+AGG 0.467136 2_1:+55704966 None:intergenic
TGGTGTGTGGAATGTGGAAT+AGG 0.467678 2_1:+55705389 None:intergenic
AGGTTGGAGATGTTAAGTGC+AGG 0.468925 2_1:-55704341 Msa0213130:three_prime_UTR
GGTTCATTTGTAACAACAAA+GGG 0.474781 2_1:+55705108 None:intergenic
AAATTGTTGGAGCAAGAATT+GGG 0.479522 2_1:-55704601 Msa0213130:CDS
AGTGATTCTGCTTGATTCCT+GGG 0.480041 2_1:-55705219 Msa0213130:CDS
GACATTTGTTAAAATTATGA+TGG 0.484695 2_1:-55704269 Msa0213130:three_prime_UTR
GAGATCACAAGCTAGGTTCT+GGG 0.491244 2_1:-55704958 Msa0213130:CDS
GTCACTCCCTGATCAGGATA+AGG 0.500395 2_1:-55704407 Msa0213130:CDS
GGCTGATTATGTTGATAAGA+AGG 0.507102 2_1:-55704937 Msa0213130:intron
TGAAGGTGGAAGGAACCTTT+GGG 0.507522 2_1:-55704677 Msa0213130:CDS
AGAACCTTTATTTATAGAGT+GGG 0.514332 2_1:+55705341 None:intergenic
ATTCTGAGTCTCATGCCAAA+TGG 0.515676 2_1:+55705191 None:intergenic
ATTGTAGAGCTGAGGAAGAC+GGG 0.518205 2_1:-55704373 Msa0213130:CDS
ATGCCCTAAGATCATTGCTT+GGG 0.524861 2_1:-55704464 Msa0213130:CDS
AATGTCAAATAGATATATGA+AGG 0.531559 2_1:-55704694 Msa0213130:intron
AGAATAGCCCTAGCTGAAAA+GGG 0.531819 2_1:-55705173 Msa0213130:CDS
AATGCCCTAAGATCATTGCT+TGG 0.533110 2_1:-55704465 Msa0213130:CDS
CATGCCAAATGGACTTGCCC+AGG 0.533387 2_1:+55705202 None:intergenic
AATTGTAGAGCTGAGGAAGA+CGG 0.540044 2_1:-55704374 Msa0213130:CDS
TTATTCTCTCTGACTAGCCA+TGG 0.541830 2_1:-55705249 Msa0213130:exon
AGGAAGACGGGTTATTGAGT+AGG 0.546317 2_1:-55704361 Msa0213130:exon
TATCTCCTCCAAAATAAGTC+CGG 0.546574 2_1:+55704575 None:intergenic
CTTATCAGAGATCACAAGCT+AGG 0.551577 2_1:-55704965 Msa0213130:CDS
AATAGATAGATTGTTATGGA+AGG 0.553152 2_1:+55705289 None:intergenic
TGGAGCAAGAATTGGGAGAC+CGG 0.556673 2_1:-55704594 Msa0213130:CDS
ATCCCTGTTCTGATTCATAA+CGG 0.561977 2_1:-55705071 Msa0213130:CDS
TGCTGTTCAGTATATTGATG+AGG 0.563629 2_1:-55705024 Msa0213130:CDS
AGGAAGGAATAGTGACTGTG+AGG 0.567594 2_1:+55705409 None:intergenic
AATAGATATATGAAGGTGGA+AGG 0.568115 2_1:-55704687 Msa0213130:intron
TGGCAACATCAGTGTAGAGA+AGG 0.581888 2_1:-55704488 Msa0213130:CDS
TAAGTGCAGGTTTAGATGAA+CGG 0.587945 2_1:-55704328 Msa0213130:three_prime_UTR
CAGAATCACTTCATCAGCCA+TGG 0.592382 2_1:+55705232 None:intergenic
GAAATGTGGTGTGTGGAATG+TGG 0.594195 2_1:+55705383 None:intergenic
CTCTACACTGATGTTGCCAA+AGG 0.596162 2_1:+55704492 None:intergenic
GATCTCTGATAAGGATCAGA+AGG 0.596783 2_1:+55704975 None:intergenic
GGGTTCATTTGTAACAACAA+AGG 0.598423 2_1:+55705107 None:intergenic
CATGGACCTTATCCTGATCA+GGG 0.606504 2_1:+55704401 None:intergenic
GTCAAATAGATATATGAAGG+TGG 0.608108 2_1:-55704691 Msa0213130:intron
TAAGGATCAGAAGGCAACAA+AGG 0.613337 2_1:+55704984 None:intergenic
TCAGCTCTACAATTAAGTCA+TGG 0.618125 2_1:+55704383 None:intergenic
TACCGTTATGAATCAGAACA+GGG 0.629965 2_1:+55705069 None:intergenic
GTGTGGAATGTGGAATAGGA+AGG 0.630377 2_1:+55705393 None:intergenic
ATGACTTAATTGTAGAGCTG+AGG 0.630926 2_1:-55704381 Msa0213130:CDS
TAGTATAGAAATGTGGTGTG+TGG 0.643154 2_1:+55705376 None:intergenic
ATGTGTGTGAGAGAGTACAA+AGG 0.665942 2_1:+55705316 None:intergenic
GAATGAATAGTATAGAAATG+TGG 0.685822 2_1:+55705369 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAGAATAGATAGATTGTTA+TGG + chr2_1:55704293-55704312 None:intergenic 20.0%
!!! TGATAAGAAGGTATATAAAT+TGG - chr2_1:55704650-55704669 Msa0213130:CDS 20.0%
!!! AGTTTTATATGAGACTTATA+TGG - chr2_1:55704801-55704820 Msa0213130:intron 20.0%
!! AATGTCAAATAGATATATGA+AGG - chr2_1:55704881-55704900 Msa0213130:intron 20.0%
!!! GACATTTGTTAAAATTATGA+TGG - chr2_1:55705306-55705325 Msa0213130:five_prime_UTR 20.0%
!!! TAATAAAAAGGCACTTATAT+GGG - chr2_1:55705420-55705439 Msa0213130:five_prime_UTR 20.0%
!! ATAGAATAGATAGATTGTTA+TGG + chr2_1:55704293-55704312 None:intergenic 20.0%
!!! TGATAAGAAGGTATATAAAT+TGG - chr2_1:55704650-55704669 Msa0213130:CDS 20.0%
!!! AGTTTTATATGAGACTTATA+TGG - chr2_1:55704801-55704820 Msa0213130:intron 20.0%
!! AATGTCAAATAGATATATGA+AGG - chr2_1:55704881-55704900 Msa0213130:intron 20.0%
!!! GACATTTGTTAAAATTATGA+TGG - chr2_1:55705306-55705325 Msa0213130:five_prime_UTR 20.0%
!!! TAATAAAAAGGCACTTATAT+GGG - chr2_1:55705420-55705439 Msa0213130:five_prime_UTR 20.0%
! TATGTTGAATTGCACAATAT+AGG - chr2_1:55704117-55704136 Msa0213130:three_prime_UTR 25.0%
! GAATGAATAGTATAGAAATG+TGG + chr2_1:55704209-55704228 None:intergenic 25.0%
! TTTATTTATAGAGTGGGATT+TGG + chr2_1:55704231-55704250 None:intergenic 25.0%
! AAATCCCACTCTATAAATAA+AGG - chr2_1:55704230-55704249 Msa0213130:three_prime_UTR 25.0%
!! AGAACCTTTATTTATAGAGT+GGG + chr2_1:55704237-55704256 None:intergenic 25.0%
!! TAGAACCTTTATTTATAGAG+TGG + chr2_1:55704238-55704257 None:intergenic 25.0%
! AATAGATAGATTGTTATGGA+AGG + chr2_1:55704289-55704308 None:intergenic 25.0%
! TATGATGGTTTATTGTGATT+TGG - chr2_1:55705321-55705340 Msa0213130:five_prime_UTR 25.0%
! TAAATCATTTATGACGTGTT+TGG + chr2_1:55705361-55705380 None:intergenic 25.0%
!!! GTAATAAAAAGGCACTTATA+TGG - chr2_1:55705419-55705438 Msa0213130:five_prime_UTR 25.0%
! TATGTTGAATTGCACAATAT+AGG - chr2_1:55704117-55704136 Msa0213130:three_prime_UTR 25.0%
! GAATGAATAGTATAGAAATG+TGG + chr2_1:55704209-55704228 None:intergenic 25.0%
! TTTATTTATAGAGTGGGATT+TGG + chr2_1:55704231-55704250 None:intergenic 25.0%
! AAATCCCACTCTATAAATAA+AGG - chr2_1:55704230-55704249 Msa0213130:three_prime_UTR 25.0%
!! AGAACCTTTATTTATAGAGT+GGG + chr2_1:55704237-55704256 None:intergenic 25.0%
!! TAGAACCTTTATTTATAGAG+TGG + chr2_1:55704238-55704257 None:intergenic 25.0%
! AATAGATAGATTGTTATGGA+AGG + chr2_1:55704289-55704308 None:intergenic 25.0%
! TATGATGGTTTATTGTGATT+TGG - chr2_1:55705321-55705340 Msa0213130:five_prime_UTR 25.0%
! TAAATCATTTATGACGTGTT+TGG + chr2_1:55705361-55705380 None:intergenic 25.0%
!!! GTAATAAAAAGGCACTTATA+TGG - chr2_1:55705419-55705438 Msa0213130:five_prime_UTR 25.0%
GGTTCATTTGTAACAACAAA+GGG + chr2_1:55704470-55704489 None:intergenic 30.0%
GCAACTAAAGATTCACAAAT+AGG + chr2_1:55704534-55704553 None:intergenic 30.0%
TTCAGTATATTGATGAGGTT+TGG - chr2_1:55704556-55704575 Msa0213130:CDS 30.0%
GTCAAATAGATATATGAAGG+TGG - chr2_1:55704884-55704903 Msa0213130:intron 30.0%
AATAGATATATGAAGGTGGA+AGG - chr2_1:55704888-55704907 Msa0213130:intron 30.0%
GACATCTATGAATTCTTTCT+TGG + chr2_1:55704951-55704970 None:intergenic 30.0%
AAATTGTTGGAGCAAGAATT+GGG - chr2_1:55704974-55704993 Msa0213130:CDS 30.0%
! CTTTTAAACCAAGTGTAGAA+TGG + chr2_1:55705055-55705074 None:intergenic 30.0%
TTTAAAAGCTATGAGACCTT+TGG - chr2_1:55705067-55705086 Msa0213130:CDS 30.0%
GGTTCATTTGTAACAACAAA+GGG + chr2_1:55704470-55704489 None:intergenic 30.0%
GCAACTAAAGATTCACAAAT+AGG + chr2_1:55704534-55704553 None:intergenic 30.0%
TTCAGTATATTGATGAGGTT+TGG - chr2_1:55704556-55704575 Msa0213130:CDS 30.0%
GTCAAATAGATATATGAAGG+TGG - chr2_1:55704884-55704903 Msa0213130:intron 30.0%
AATAGATATATGAAGGTGGA+AGG - chr2_1:55704888-55704907 Msa0213130:intron 30.0%
GACATCTATGAATTCTTTCT+TGG + chr2_1:55704951-55704970 None:intergenic 30.0%
AAATTGTTGGAGCAAGAATT+GGG - chr2_1:55704974-55704993 Msa0213130:CDS 30.0%
! CTTTTAAACCAAGTGTAGAA+TGG + chr2_1:55705055-55705074 None:intergenic 30.0%
TTTAAAAGCTATGAGACCTT+TGG - chr2_1:55705067-55705086 Msa0213130:CDS 30.0%
TAGTATAGAAATGTGGTGTG+TGG + chr2_1:55704202-55704221 None:intergenic 35.0%
GGGTTCATTTGTAACAACAA+AGG + chr2_1:55704471-55704490 None:intergenic 35.0%
ATCCCTGTTCTGATTCATAA+CGG - chr2_1:55704504-55704523 Msa0213130:CDS 35.0%
TACCGTTATGAATCAGAACA+GGG + chr2_1:55704509-55704528 None:intergenic 35.0%
TTACCGTTATGAATCAGAAC+AGG + chr2_1:55704510-55704529 None:intergenic 35.0%
TGCTGTTCAGTATATTGATG+AGG - chr2_1:55704551-55704570 Msa0213130:CDS 35.0%
! GGCTGATTATGTTGATAAGA+AGG - chr2_1:55704638-55704657 Msa0213130:CDS 35.0%
CATAGATGTCCTCAAATTGT+TGG - chr2_1:55704961-55704980 Msa0213130:CDS 35.0%
ATTCTTGCTCCAACAATTTG+AGG + chr2_1:55704973-55704992 None:intergenic 35.0%
CAAATTGTTGGAGCAAGAAT+TGG - chr2_1:55704973-55704992 Msa0213130:CDS 35.0%
TATCTCCTCCAAAATAAGTC+CGG + chr2_1:55705003-55705022 None:intergenic 35.0%
!! TATTTTGGAGGAGATAAGCT+TGG - chr2_1:55705007-55705026 Msa0213130:CDS 35.0%
CACTTATTCCATTCTACACT+TGG - chr2_1:55705044-55705063 Msa0213130:CDS 35.0%
TCAGCTCTACAATTAAGTCA+TGG + chr2_1:55705195-55705214 None:intergenic 35.0%
ATGACTTAATTGTAGAGCTG+AGG - chr2_1:55705194-55705213 Msa0213130:CDS 35.0%
TAAGTGCAGGTTTAGATGAA+CGG - chr2_1:55705247-55705266 Msa0213130:exon 35.0%
CTTACAAAATGCACAACGTT+TGG + chr2_1:55705446-55705465 None:intergenic 35.0%
TAGTATAGAAATGTGGTGTG+TGG + chr2_1:55704202-55704221 None:intergenic 35.0%
GGGTTCATTTGTAACAACAA+AGG + chr2_1:55704471-55704490 None:intergenic 35.0%
ATCCCTGTTCTGATTCATAA+CGG - chr2_1:55704504-55704523 Msa0213130:CDS 35.0%
TACCGTTATGAATCAGAACA+GGG + chr2_1:55704509-55704528 None:intergenic 35.0%
TTACCGTTATGAATCAGAAC+AGG + chr2_1:55704510-55704529 None:intergenic 35.0%
TGCTGTTCAGTATATTGATG+AGG - chr2_1:55704551-55704570 Msa0213130:CDS 35.0%
! GGCTGATTATGTTGATAAGA+AGG - chr2_1:55704638-55704657 Msa0213130:CDS 35.0%
CATAGATGTCCTCAAATTGT+TGG - chr2_1:55704961-55704980 Msa0213130:CDS 35.0%
ATTCTTGCTCCAACAATTTG+AGG + chr2_1:55704973-55704992 None:intergenic 35.0%
CAAATTGTTGGAGCAAGAAT+TGG - chr2_1:55704973-55704992 Msa0213130:CDS 35.0%
TATCTCCTCCAAAATAAGTC+CGG + chr2_1:55705003-55705022 None:intergenic 35.0%
!! TATTTTGGAGGAGATAAGCT+TGG - chr2_1:55705007-55705026 Msa0213130:CDS 35.0%
CACTTATTCCATTCTACACT+TGG - chr2_1:55705044-55705063 Msa0213130:CDS 35.0%
TCAGCTCTACAATTAAGTCA+TGG + chr2_1:55705195-55705214 None:intergenic 35.0%
ATGACTTAATTGTAGAGCTG+AGG - chr2_1:55705194-55705213 Msa0213130:CDS 35.0%
TAAGTGCAGGTTTAGATGAA+CGG - chr2_1:55705247-55705266 Msa0213130:exon 35.0%
CTTACAAAATGCACAACGTT+TGG + chr2_1:55705446-55705465 None:intergenic 35.0%
! ATGTGTGTGAGAGAGTACAA+AGG + chr2_1:55704262-55704281 None:intergenic 40.0%
! TTATTCTCTCTGACTAGCCA+TGG - chr2_1:55704326-55704345 Msa0213130:three_prime_UTR 40.0%
AAGTGATTCTGCTTGATTCC+TGG - chr2_1:55704355-55704374 Msa0213130:exon 40.0%
AGTGATTCTGCTTGATTCCT+GGG - chr2_1:55704356-55704375 Msa0213130:exon 40.0%
ATTCTGAGTCTCATGCCAAA+TGG + chr2_1:55704387-55704406 None:intergenic 40.0%
AGAATAGCCCTAGCTGAAAA+GGG - chr2_1:55704402-55704421 Msa0213130:CDS 40.0%
! CTTGATACCCTTTTCAGCTA+GGG + chr2_1:55704412-55704431 None:intergenic 40.0%
! CAGGGATTTTCTTGTGAACA+GGG + chr2_1:55704491-55704510 None:intergenic 40.0%
! ACAGGGATTTTCTTGTGAAC+AGG + chr2_1:55704492-55704511 None:intergenic 40.0%
TAAGGATCAGAAGGCAACAA+AGG + chr2_1:55704594-55704613 None:intergenic 40.0%
GATCTCTGATAAGGATCAGA+AGG + chr2_1:55704603-55704622 None:intergenic 40.0%
! CTAGCTTGTGATCTCTGATA+AGG + chr2_1:55704612-55704631 None:intergenic 40.0%
CTTATCAGAGATCACAAGCT+AGG - chr2_1:55704610-55704629 Msa0213130:CDS 40.0%
! TTCTTGCTCTTCTCCCTTTT+TGG + chr2_1:55704924-55704943 None:intergenic 40.0%
AATGCCCTAAGATCATTGCT+TGG - chr2_1:55705110-55705129 Msa0213130:CDS 40.0%
ATGCCCTAAGATCATTGCTT+GGG - chr2_1:55705111-55705130 Msa0213130:CDS 40.0%
CTCAATCTGCATACATCTCT+TGG + chr2_1:55705137-55705156 None:intergenic 40.0%
AATTGTAGAGCTGAGGAAGA+CGG - chr2_1:55705201-55705220 Msa0213130:CDS 40.0%
GTTTAGATGAACGGTGAGAT+TGG - chr2_1:55705256-55705275 Msa0213130:five_prime_UTR 40.0%
! ATGTGTGTGAGAGAGTACAA+AGG + chr2_1:55704262-55704281 None:intergenic 40.0%
! TTATTCTCTCTGACTAGCCA+TGG - chr2_1:55704326-55704345 Msa0213130:three_prime_UTR 40.0%
AAGTGATTCTGCTTGATTCC+TGG - chr2_1:55704355-55704374 Msa0213130:exon 40.0%
AGTGATTCTGCTTGATTCCT+GGG - chr2_1:55704356-55704375 Msa0213130:exon 40.0%
ATTCTGAGTCTCATGCCAAA+TGG + chr2_1:55704387-55704406 None:intergenic 40.0%
AGAATAGCCCTAGCTGAAAA+GGG - chr2_1:55704402-55704421 Msa0213130:CDS 40.0%
! CTTGATACCCTTTTCAGCTA+GGG + chr2_1:55704412-55704431 None:intergenic 40.0%
! CAGGGATTTTCTTGTGAACA+GGG + chr2_1:55704491-55704510 None:intergenic 40.0%
! ACAGGGATTTTCTTGTGAAC+AGG + chr2_1:55704492-55704511 None:intergenic 40.0%
TAAGGATCAGAAGGCAACAA+AGG + chr2_1:55704594-55704613 None:intergenic 40.0%
GATCTCTGATAAGGATCAGA+AGG + chr2_1:55704603-55704622 None:intergenic 40.0%
! CTAGCTTGTGATCTCTGATA+AGG + chr2_1:55704612-55704631 None:intergenic 40.0%
CTTATCAGAGATCACAAGCT+AGG - chr2_1:55704610-55704629 Msa0213130:CDS 40.0%
! TTCTTGCTCTTCTCCCTTTT+TGG + chr2_1:55704924-55704943 None:intergenic 40.0%
AATGCCCTAAGATCATTGCT+TGG - chr2_1:55705110-55705129 Msa0213130:CDS 40.0%
ATGCCCTAAGATCATTGCTT+GGG - chr2_1:55705111-55705130 Msa0213130:CDS 40.0%
CTCAATCTGCATACATCTCT+TGG + chr2_1:55705137-55705156 None:intergenic 40.0%
AATTGTAGAGCTGAGGAAGA+CGG - chr2_1:55705201-55705220 Msa0213130:CDS 40.0%
GTTTAGATGAACGGTGAGAT+TGG - chr2_1:55705256-55705275 Msa0213130:five_prime_UTR 40.0%
AGGAAGGAATAGTGACTGTG+AGG + chr2_1:55704169-55704188 None:intergenic 45.0%
GTGTGGAATGTGGAATAGGA+AGG + chr2_1:55704185-55704204 None:intergenic 45.0%
TGGTGTGTGGAATGTGGAAT+AGG + chr2_1:55704189-55704208 None:intergenic 45.0%
GAAATGTGGTGTGTGGAATG+TGG + chr2_1:55704195-55704214 None:intergenic 45.0%
CAGAATCACTTCATCAGCCA+TGG + chr2_1:55704346-55704365 None:intergenic 45.0%
CAGAATAGCCCTAGCTGAAA+AGG - chr2_1:55704401-55704420 Msa0213130:CDS 45.0%
! GCTTGATACCCTTTTCAGCT+AGG + chr2_1:55704413-55704432 None:intergenic 45.0%
AGAGATCACAAGCTAGGTTC+TGG - chr2_1:55704616-55704635 Msa0213130:CDS 45.0%
GAGATCACAAGCTAGGTTCT+GGG - chr2_1:55704617-55704636 Msa0213130:CDS 45.0%
ATGAAGGTGGAAGGAACCTT+TGG - chr2_1:55704897-55704916 Msa0213130:intron 45.0%
TGAAGGTGGAAGGAACCTTT+GGG - chr2_1:55704898-55704917 Msa0213130:intron 45.0%
AAGGAACCTTTGGGCCAAAA+AGG - chr2_1:55704907-55704926 Msa0213130:intron 45.0%
AGGAACCTTTGGGCCAAAAA+GGG - chr2_1:55704908-55704927 Msa0213130:intron 45.0%
! TTGGGAGACCGGACTTATTT+TGG - chr2_1:55704992-55705011 Msa0213130:CDS 45.0%
CTCTACACTGATGTTGCCAA+AGG + chr2_1:55705086-55705105 None:intergenic 45.0%
TGGCAACATCAGTGTAGAGA+AGG - chr2_1:55705087-55705106 Msa0213130:CDS 45.0%
TGGCCCAAGCAATGATCTTA+GGG + chr2_1:55705117-55705136 None:intergenic 45.0%
TTGGCCCAAGCAATGATCTT+AGG + chr2_1:55705118-55705137 None:intergenic 45.0%
TTCAAAGTCACTCCCTGATC+AGG - chr2_1:55705162-55705181 Msa0213130:CDS 45.0%
CATGGACCTTATCCTGATCA+GGG + chr2_1:55705177-55705196 None:intergenic 45.0%
TCATGGACCTTATCCTGATC+AGG + chr2_1:55705178-55705197 None:intergenic 45.0%
ATTGTAGAGCTGAGGAAGAC+GGG - chr2_1:55705202-55705221 Msa0213130:CDS 45.0%
!! AGGAAGACGGGTTATTGAGT+AGG - chr2_1:55705214-55705233 Msa0213130:CDS 45.0%
!! AGACGGGTTATTGAGTAGGT+TGG - chr2_1:55705218-55705237 Msa0213130:CDS 45.0%
AGGTTGGAGATGTTAAGTGC+AGG - chr2_1:55705234-55705253 Msa0213130:exon 45.0%
AGGAAGGAATAGTGACTGTG+AGG + chr2_1:55704169-55704188 None:intergenic 45.0%
GTGTGGAATGTGGAATAGGA+AGG + chr2_1:55704185-55704204 None:intergenic 45.0%
TGGTGTGTGGAATGTGGAAT+AGG + chr2_1:55704189-55704208 None:intergenic 45.0%
GAAATGTGGTGTGTGGAATG+TGG + chr2_1:55704195-55704214 None:intergenic 45.0%
CAGAATCACTTCATCAGCCA+TGG + chr2_1:55704346-55704365 None:intergenic 45.0%
CAGAATAGCCCTAGCTGAAA+AGG - chr2_1:55704401-55704420 Msa0213130:CDS 45.0%
! GCTTGATACCCTTTTCAGCT+AGG + chr2_1:55704413-55704432 None:intergenic 45.0%
AGAGATCACAAGCTAGGTTC+TGG - chr2_1:55704616-55704635 Msa0213130:CDS 45.0%
GAGATCACAAGCTAGGTTCT+GGG - chr2_1:55704617-55704636 Msa0213130:CDS 45.0%
ATGAAGGTGGAAGGAACCTT+TGG - chr2_1:55704897-55704916 Msa0213130:intron 45.0%
TGAAGGTGGAAGGAACCTTT+GGG - chr2_1:55704898-55704917 Msa0213130:intron 45.0%
AAGGAACCTTTGGGCCAAAA+AGG - chr2_1:55704907-55704926 Msa0213130:intron 45.0%
AGGAACCTTTGGGCCAAAAA+GGG - chr2_1:55704908-55704927 Msa0213130:intron 45.0%
! TTGGGAGACCGGACTTATTT+TGG - chr2_1:55704992-55705011 Msa0213130:CDS 45.0%
CTCTACACTGATGTTGCCAA+AGG + chr2_1:55705086-55705105 None:intergenic 45.0%
TGGCAACATCAGTGTAGAGA+AGG - chr2_1:55705087-55705106 Msa0213130:CDS 45.0%
TGGCCCAAGCAATGATCTTA+GGG + chr2_1:55705117-55705136 None:intergenic 45.0%
TTGGCCCAAGCAATGATCTT+AGG + chr2_1:55705118-55705137 None:intergenic 45.0%
TTCAAAGTCACTCCCTGATC+AGG - chr2_1:55705162-55705181 Msa0213130:CDS 45.0%
CATGGACCTTATCCTGATCA+GGG + chr2_1:55705177-55705196 None:intergenic 45.0%
TCATGGACCTTATCCTGATC+AGG + chr2_1:55705178-55705197 None:intergenic 45.0%
ATTGTAGAGCTGAGGAAGAC+GGG - chr2_1:55705202-55705221 Msa0213130:CDS 45.0%
!! AGGAAGACGGGTTATTGAGT+AGG - chr2_1:55705214-55705233 Msa0213130:CDS 45.0%
!! AGACGGGTTATTGAGTAGGT+TGG - chr2_1:55705218-55705237 Msa0213130:CDS 45.0%
AGGTTGGAGATGTTAAGTGC+AGG - chr2_1:55705234-55705253 Msa0213130:exon 45.0%
!! TATTATATTATGTAATAAAA+AGG - chr2_1:55705408-55705427 Msa0213130:five_prime_UTR 5.0%
!! TATTATATTATGTAATAAAA+AGG - chr2_1:55705408-55705427 Msa0213130:five_prime_UTR 5.0%
GATTCCTGGGCAAGTCCATT+TGG - chr2_1:55704369-55704388 Msa0213130:CDS 50.0%
!! CTTCTCCCTTTTTGGCCCAA+AGG + chr2_1:55704916-55704935 None:intergenic 50.0%
TGGAGCAAGAATTGGGAGAC+CGG - chr2_1:55704981-55705000 Msa0213130:CDS 50.0%
!!! GGAGACCGGACTTATTTTGG+AGG - chr2_1:55704995-55705014 Msa0213130:CDS 50.0%
GTCACTCCCTGATCAGGATA+AGG - chr2_1:55705168-55705187 Msa0213130:CDS 50.0%
GATTCCTGGGCAAGTCCATT+TGG - chr2_1:55704369-55704388 Msa0213130:CDS 50.0%
!! CTTCTCCCTTTTTGGCCCAA+AGG + chr2_1:55704916-55704935 None:intergenic 50.0%
TGGAGCAAGAATTGGGAGAC+CGG - chr2_1:55704981-55705000 Msa0213130:CDS 50.0%
!!! GGAGACCGGACTTATTTTGG+AGG - chr2_1:55704995-55705014 Msa0213130:CDS 50.0%
GTCACTCCCTGATCAGGATA+AGG - chr2_1:55705168-55705187 Msa0213130:CDS 50.0%
! CATGCCAAATGGACTTGCCC+AGG + chr2_1:55704376-55704395 None:intergenic 55.0%
! CATGCCAAATGGACTTGCCC+AGG + chr2_1:55704376-55704395 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 55704109 55705488 55704109 ID=Msa0213130;Name=Msa0213130
chr2_1 mRNA 55704109 55705488 55704109 ID=Msa0213130-mRNA-1;Parent=Msa0213130;Name=Msa0213130-mRNA-1;_AED=0.05;_eAED=0.05;_QI=236|1|1|1|1|1|2|257|217
chr2_1 exon 55704109 55704704 55704109 ID=Msa0213130-mRNA-1:exon:15480;Parent=Msa0213130-mRNA-1
chr2_1 exon 55704938 55705488 55704938 ID=Msa0213130-mRNA-1:exon:15479;Parent=Msa0213130-mRNA-1
chr2_1 five_prime_UTR 55705253 55705488 55705253 ID=Msa0213130-mRNA-1:five_prime_utr;Parent=Msa0213130-mRNA-1
chr2_1 CDS 55704938 55705252 55704938 ID=Msa0213130-mRNA-1:cds;Parent=Msa0213130-mRNA-1
chr2_1 CDS 55704366 55704704 55704366 ID=Msa0213130-mRNA-1:cds;Parent=Msa0213130-mRNA-1
chr2_1 three_prime_UTR 55704109 55704365 55704109 ID=Msa0213130-mRNA-1:three_prime_utr;Parent=Msa0213130-mRNA-1
Gene Sequence

>Msa0213130

ATGGCTGATGAAGTGATTCTGCTTGATTCCTGGGCAAGTCCATTTGGCATGAGACTCAGAATAGCCCTAGCTGAAAAGGGTATCAAGCATGAGTACAAAGAAGAAGATCTAAGTAACAAAAGCCCTTTGTTGTTACAAATGAACCCTGTTCACAAGAAAATCCCTGTTCTGATTCATAACGGTAAACCTATTTGTGAATCTTTAGTTGCTGTTCAGTATATTGATGAGGTTTGGAATGAGAAATCTCCTTTGTTGCCTTCTGATCCTTATCAGAGATCACAAGCTAGGTTCTGGGCTGATTATGTTGATAAGAAGATATATGAAGGTGGAAGGAACCTTTGGGCCAAAAAGGGAGAAGAGCAAGAAGTTGCCAAGAAAGAATTCATAGATGTCCTCAAATTGTTGGAGCAAGAATTGGGAGACCGGACTTATTTTGGAGGAGATAAGCTTGGTTTTGTTGATGTAGCACTTATTCCATTCTACACTTGGTTTAAAAGCTATGAGACCTTTGGCAACATCAGTGTAGAGAAGGAATGCCCTAAGATCATTGCTTGGGCCAAGAGATGTATGCAGATTGAGAGTGTTTCAAAGTCACTCCCTGATCAGGATAAGGTCCATGACTTAATTGTAGAGCTGAGGAAGACGGGTTATTGA
Protein sequence

>Msa0213130

MADEVILLDSWASPFGMRLRIALAEKGIKHEYKEEDLSNKSPLLLQMNPVHKKIPVLIHNGKPICESLVAVQYIDEVWNEKSPLLPSDPYQRSQARFWADYVDKKIYEGGRNLWAKKGEEQEVAKKEFIDVLKLLEQELGDRTYFGGDKLGFVDVALIPFYTWFKSYETFGNISVEKECPKIIAWAKRCMQIESVSKSLPDQDKVHDLIVELRKTGY*