AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0214640

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0214640 MtrunA17_Chr2g0315641 97.890 237 5 0 22 258 135 371 4.27e-175 486
Msa0214640 MtrunA17_Chr2g0315651 77.778 243 47 4 20 258 128 367 2.40e-127 365
Msa0214640 MtrunA17_Chr2g0286441 52.941 272 98 6 15 258 124 393 2.67e-91 275
Msa0214640 MtrunA17_Chr5g0404321 50.741 270 101 3 21 258 147 416 2.08e-88 268
Msa0214640 MtrunA17_Chr1g0201891 48.639 294 100 6 15 258 139 431 2.65e-86 263
Msa0214640 MtrunA17_Chr8g0373001 50.000 268 104 3 21 258 145 412 4.63e-84 257
Msa0214640 MtrunA17_Chr1g0196071 52.747 273 95 7 15 258 135 402 5.66e-84 256
Msa0214640 MtrunA17_Chr2g0278511 48.966 290 100 8 15 258 140 427 9.82e-83 254
Msa0214640 MtrunA17_Chr1g0155301 50.763 262 106 7 20 258 161 422 2.63e-81 250
Msa0214640 MtrunA17_Chr8g0373361 36.226 265 126 9 21 251 150 405 7.38e-39 139
Msa0214640 MtrunA17_Chr8g0373331 42.439 205 94 9 67 251 148 348 5.08e-35 128
Msa0214640 MtrunA17_Chr8g0373351 39.109 202 107 7 61 250 89 286 8.95e-31 115
Msa0214640 MtrunA17_Chr8g0373341 36.100 241 87 5 28 249 129 321 1.29e-28 110
Msa0214640 MtrunA17_Chr1g0156151 45.946 74 40 0 23 96 149 222 1.77e-15 73.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0214640 AT1G53320.1 81.633 245 39 2 20 258 135 379 2.38e-141 402
Msa0214640 AT2G47900.2 50.000 270 107 4 15 258 139 406 9.52e-87 264
Msa0214640 AT2G47900.1 50.000 270 107 4 15 258 139 406 9.52e-87 264
Msa0214640 AT2G47900.3 50.951 263 101 4 22 258 147 407 2.21e-86 263
Msa0214640 AT2G18280.2 49.805 257 107 4 21 258 140 393 1.16e-80 248
Msa0214640 AT2G18280.1 49.805 257 107 4 21 258 140 393 1.16e-80 248
Msa0214640 AT1G25280.1 47.351 302 100 8 15 258 145 445 1.91e-80 249
Msa0214640 AT1G76900.2 43.631 314 102 7 17 258 145 455 1.19e-77 242
Msa0214640 AT1G76900.1 43.631 314 102 7 17 258 145 455 1.19e-77 242
Msa0214640 AT1G76900.3 43.631 314 102 7 17 258 145 455 1.19e-77 242
Msa0214640 AT5G18680.2 48.077 260 109 6 22 258 41 297 2.59e-74 228
Msa0214640 AT1G43640.2 45.017 291 110 6 15 258 76 363 7.61e-74 229
Msa0214640 AT1G25280.3 46.642 268 85 7 48 258 1 267 1.57e-73 225
Msa0214640 AT1G25280.2 46.642 268 85 7 48 258 1 267 1.57e-73 225
Msa0214640 AT1G43640.1 45.017 291 110 6 15 258 142 429 2.95e-73 230
Msa0214640 AT3G06380.2 46.388 263 106 6 22 258 35 288 4.07e-73 225
Msa0214640 AT5G18680.1 48.263 259 110 6 22 258 133 389 4.10e-73 228
Msa0214640 AT3G06380.1 46.388 263 106 6 22 258 127 380 4.27e-72 225
Msa0214640 AT1G47270.3 47.529 263 105 8 20 258 135 388 4.41e-71 223
Msa0214640 AT1G47270.1 47.529 263 105 8 20 258 160 413 6.27e-71 223
Msa0214640 AT1G47270.2 47.529 263 105 8 20 258 159 412 6.95e-71 223

Find 130 sgRNAs with CRISPR-Local

Find 191 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCAATTCTTGAGCAATTAA+TGG 0.137569 2_1:+59197105 Msa0214640:five_prime_UTR
AGAAATTTCATTAATATATA+AGG 0.156836 2_1:+59196612 Msa0214640:five_prime_UTR
TACTTAATTCTATCATCTTT+TGG 0.202042 2_1:+59196759 Msa0214640:five_prime_UTR
CTTCACAGCTCGGACTTTCT+TGG 0.211432 2_1:+59198464 Msa0214640:intron
ATGATGGTTTGGCACCATTA+TGG 0.232651 2_1:-59198520 None:intergenic
GCAGCCCTTGTCCGCCTTCC+AGG 0.253322 2_1:+59198982 Msa0214640:CDS
GCGATATAACATACTCAGTA+TGG 0.267086 2_1:-59197481 None:intergenic
TCCATTAATTGCTCAAGAAT+TGG 0.282705 2_1:-59197106 None:intergenic
CTTCAAAAGGTTGAACTTGT+AGG 0.287891 2_1:-59198624 None:intergenic
CATTATGCTTATTTATTCCT+TGG 0.313752 2_1:+59199069 Msa0214640:three_prime_UTR
GAAATTTCATTAATATATAA+GGG 0.315519 2_1:+59196613 Msa0214640:five_prime_UTR
GCCATTTGCCTAACTAGCTT+TGG 0.331794 2_1:+59199010 Msa0214640:CDS
ACTACTACAGGAAAAGAAAA+AGG 0.332319 2_1:-59197021 None:intergenic
ACAAATCAGAGTATGCATTT+AGG 0.336213 2_1:-59196689 None:intergenic
ATGTTTACAGTAAATAATAT+TGG 0.340939 2_1:-59197130 None:intergenic
TATAGACTCAGCCAAGTAAA+AGG 0.342906 2_1:-59199130 None:intergenic
TGGCACCATTATGGGGTGGC+TGG 0.351579 2_1:-59198511 None:intergenic
AACTAGCTTTGGCACTAAAC+TGG 0.354421 2_1:+59199021 Msa0214640:CDS
GTTTAGTGCCAAAGCTAGTT+AGG 0.355030 2_1:-59199018 None:intergenic
TTGAGGGCTTATCTGTTTGC+TGG 0.356555 2_1:-59198567 None:intergenic
GTCGATTGATAACACTAGTT+TGG 0.361190 2_1:+59196465 Msa0214640:five_prime_UTR
ACTGACTGCAAGCCTGTATC+AGG 0.363573 2_1:-59196976 None:intergenic
TCGCCAGCATTCACAGATAA+AGG 0.367850 2_1:+59197419 Msa0214640:CDS
TGAGTCTATAATTTGATTGT+AGG 0.371331 2_1:+59199143 Msa0214640:three_prime_UTR
TTGGCCCTCTTGACTTCAAA+AGG 0.375126 2_1:-59198637 None:intergenic
AGCTCTGGCACATGATGGTT+TGG 0.380769 2_1:-59198531 None:intergenic
GGTTTAGTGTTGTGAAATAT+CGG 0.387818 2_1:+59196374 Msa0214640:five_prime_UTR
ACATTCTTATTATGTAAATC+TGG 0.389490 2_1:+59196844 Msa0214640:five_prime_UTR
ATTTACTGTAAACATATGTT+TGG 0.392738 2_1:+59197138 Msa0214640:five_prime_UTR
TGATGGTTTGGCACCATTAT+GGG 0.393178 2_1:-59198519 None:intergenic
CTTTCCCTCCCGGTTGACTT+TGG 0.397531 2_1:-59198892 None:intergenic
AAATGCATACTCTGATTTGT+TGG 0.404017 2_1:+59196692 Msa0214640:five_prime_UTR
AATCAGCAATTTATAATTGT+CGG 0.405363 2_1:-59197303 None:intergenic
TCTTCATCTCCTTTCCCTCC+CGG 0.407140 2_1:-59198902 None:intergenic
TATTAGCAGCTAGAAGATAT+AGG 0.409814 2_1:+59197450 Msa0214640:CDS
GAAACGGTTCTCCTCCAGTT+TGG 0.411512 2_1:+59198923 Msa0214640:CDS
CGTCCCCTACTTTACCAAAC+TGG 0.415882 2_1:-59198937 None:intergenic
CATCCTATTCCTAACTAGTT+CGG 0.415986 2_1:+59196353 Msa0214640:five_prime_UTR
TACTGTGGACCAAAGTCAAC+CGG 0.421015 2_1:+59198883 Msa0214640:CDS
TATACTAATTTAGTAATTGT+AGG 0.421030 2_1:+59196871 Msa0214640:five_prime_UTR
AAACCGAACTAGTTAGGAAT+AGG 0.422108 2_1:-59196356 None:intergenic
GCAGAACAGGAAAGAGGGGC+AGG 0.423894 2_1:-59197393 None:intergenic
CTTCCATAGTCATATATTTG+TGG 0.425403 2_1:+59199223 Msa0214640:three_prime_UTR
GGCAAAACGTCGGCTAGCTC+TGG 0.426830 2_1:-59198546 None:intergenic
GAACAACTCTCTTTCAATCA+TGG 0.432368 2_1:-59197183 None:intergenic
AAAGTTTCCTGTCTCATCAA+TGG 0.435431 2_1:+59198679 Msa0214640:CDS
AACACTAAACCGAACTAGTT+AGG 0.436243 2_1:-59196362 None:intergenic
AGACCACAAATATATGACTA+TGG 0.437606 2_1:-59199226 None:intergenic
GGCACGAGCATTTGCAGTGC+TGG 0.438763 2_1:+59198801 Msa0214640:CDS
AAAATTGCTGATCCTGATAC+AGG 0.441684 2_1:+59196964 Msa0214640:five_prime_UTR
ATACCTTCACCATGGACTAT+AGG 0.445930 2_1:+59198960 Msa0214640:CDS
TCCTCCAGTTTGGTAAAGTA+GGG 0.446113 2_1:+59198933 Msa0214640:CDS
CTCCTCCAGTTTGGTAAAGT+AGG 0.453347 2_1:+59198932 Msa0214640:CDS
ATGCTGGCGATGAGCAGAAC+AGG 0.461702 2_1:-59197406 None:intergenic
AAACGCCTGGAAGGCGGACA+AGG 0.466204 2_1:-59198987 None:intergenic
CAAAGTCAACCGGGAGGGAA+AGG 0.466547 2_1:+59198893 Msa0214640:CDS
GACTTGCTTCAGTCAATGTA+TGG 0.467838 2_1:+59196306 Msa0214640:five_prime_UTR
AGAACAGGAAAGAGGGGCAG+GGG 0.471192 2_1:-59197391 None:intergenic
GTTCGTAACTGTGTGATTTG+TGG 0.472922 2_1:+59197202 Msa0214640:five_prime_UTR
GACAGCCAGCCACCCCATAA+TGG 0.478762 2_1:+59198506 Msa0214640:CDS
AAAGGAATATTCACTACTAC+AGG 0.481036 2_1:-59197033 None:intergenic
AAGTTTCCTGTCTCATCAAT+GGG 0.481200 2_1:+59198680 Msa0214640:CDS
ATTCCTTTGTAGTAGTTATG+TGG 0.487148 2_1:+59197048 Msa0214640:five_prime_UTR
AGTGAGCACATCATCCTCCT+TGG 0.487846 2_1:-59198656 None:intergenic
TGCTGGTAACTTCGAGGCAG+GGG 0.489666 2_1:+59198595 Msa0214640:CDS
TGCAAATGCTCGTGCCACCT+TGG 0.490175 2_1:-59198794 None:intergenic
GCATTTAAGCATGGTGTCCA+TGG 0.490263 2_1:+59196735 Msa0214640:five_prime_UTR
TTAGGCAAATGGCAAACGCC+TGG 0.502545 2_1:-59199000 None:intergenic
TTTCCTTTATCTGTGAATGC+TGG 0.503242 2_1:-59197422 None:intergenic
CTTTCACCCATTGATGAGAC+AGG 0.505376 2_1:-59198686 None:intergenic
ATCTGTGAAGAACTTTCAGC+TGG 0.505739 2_1:+59198856 Msa0214640:CDS
CAAGGAAGTAATGCTTATGT+TGG 0.506103 2_1:+59197524 Msa0214640:CDS
GCCAAAGCTAGTTAGGCAAA+TGG 0.507967 2_1:-59199011 None:intergenic
CTTTCAGCTGGTTGCTACTG+TGG 0.512635 2_1:+59198868 Msa0214640:CDS
CAGAACAGGAAAGAGGGGCA+GGG 0.516488 2_1:-59197392 None:intergenic
TTTATGGTGGCTCAAGATGA+TGG 0.516590 2_1:+59196417 Msa0214640:five_prime_UTR
CCTGCTGGTAACTTCGAGGC+AGG 0.517968 2_1:+59198593 Msa0214640:CDS
AAATGATGATAGTTAGTACA+AGG 0.521595 2_1:+59197262 Msa0214640:five_prime_UTR
TACTGTAAACATATGTTTGG+TGG 0.528531 2_1:+59197141 Msa0214640:five_prime_UTR
AAGTTACCAGCAGGAACTTG+AGG 0.529054 2_1:-59198584 None:intergenic
GGAGATTTCATGAGTGGCTA+AGG 0.533177 2_1:+59196636 Msa0214640:five_prime_UTR
ATGCTTATGTTGGCAAGCTA+AGG 0.535171 2_1:+59197534 Msa0214640:CDS
CAACCACATAACTACTACAA+AGG 0.537018 2_1:-59197051 None:intergenic
ATAAGCCCTCAAGTTCCTGC+TGG 0.547760 2_1:+59198578 Msa0214640:CDS
AGTTACCAGCAGGAACTTGA+GGG 0.551679 2_1:-59198583 None:intergenic
CTGCTGGTAACTTCGAGGCA+GGG 0.552365 2_1:+59198594 Msa0214640:CDS
TGCTGGTGCTTGAACTTCCA+TGG 0.555279 2_1:+59198818 Msa0214640:CDS
CGATGAGCAGAACAGGAAAG+AGG 0.556463 2_1:-59197399 None:intergenic
GATGGTTTGGCACCATTATG+GGG 0.570572 2_1:-59198518 None:intergenic
TATTCACTTTATAACATCCA+AGG 0.572080 2_1:-59199086 None:intergenic
GCTGTCGTAAATTGTGAAGT+TGG 0.573043 2_1:-59198489 None:intergenic
CGGCTAGCTCTGGCACATGA+TGG 0.573160 2_1:-59198536 None:intergenic
GATGAGCAGAACAGGAAAGA+GGG 0.573241 2_1:-59197398 None:intergenic
ATGATAGAATTAAGTAACCA+TGG 0.582710 2_1:-59196752 None:intergenic
CAAGGGCTGCCTATAGTCCA+TGG 0.582759 2_1:-59198969 None:intergenic
AGGGAAAGGAGATGAAGAAA+CGG 0.585294 2_1:+59198907 Msa0214640:CDS
AACGCCTGGAAGGCGGACAA+GGG 0.585668 2_1:-59198986 None:intergenic
AAATTTCATTAATATATAAG+GGG 0.590544 2_1:+59196614 Msa0214640:five_prime_UTR
TTCTTATTATGTAAATCTGG+AGG 0.595849 2_1:+59196847 Msa0214640:five_prime_UTR
AGTTCCTGCTGGTAACTTCG+AGG 0.598795 2_1:+59198589 Msa0214640:CDS
GCAAATGGCAAACGCCTGGA+AGG 0.600612 2_1:-59198996 None:intergenic
TATCAATCGACGAACCGTAA+CGG 0.603494 2_1:-59196453 None:intergenic
CCTGCCTCGAAGTTACCAGC+AGG 0.604926 2_1:-59198593 None:intergenic
AATTTCATTAATATATAAGG+GGG 0.605692 2_1:+59196615 Msa0214640:five_prime_UTR
ATGAGTAAATTGAACAAGGT+AGG 0.605769 2_1:-59196333 None:intergenic
ATGAGCAGAACAGGAAAGAG+GGG 0.608172 2_1:-59197397 None:intergenic
CGATATAACATACTCAGTAT+GGG 0.610140 2_1:-59197480 None:intergenic
GTGTTGTGAAATATCGGCCA+TGG 0.615482 2_1:+59196380 Msa0214640:five_prime_UTR
AATGGCAAACGCCTGGAAGG+CGG 0.622747 2_1:-59198993 None:intergenic
GGTTTGGCACCATTATGGGG+TGG 0.626155 2_1:-59198515 None:intergenic
ATGCCACTGTCACACGACCA+TGG 0.630427 2_1:-59198835 None:intergenic
CTGCCTATAGTCCATGGTGA+AGG 0.631313 2_1:-59198963 None:intergenic
AAAACAGCATGGTGCCACCA+TGG 0.631416 2_1:-59196397 None:intergenic
CCTCCAGTTTGGTAAAGTAG+GGG 0.636441 2_1:+59198934 Msa0214640:CDS
TAGGATGAGTAAATTGAACA+AGG 0.641477 2_1:-59196337 None:intergenic
TCTGTTTGCTGGCAAAACGT+CGG 0.642974 2_1:-59198556 None:intergenic
CTTCCATGGTCGTGTGACAG+TGG 0.649357 2_1:+59198832 Msa0214640:CDS
TGGACCAAAGTCAACCGGGA+GGG 0.649647 2_1:+59198888 Msa0214640:CDS
TCTTGAAGAACAAAGCTCCA+AGG 0.652245 2_1:+59198777 Msa0214640:CDS
CCCCTACTTTACCAAACTGG+AGG 0.658277 2_1:-59198934 None:intergenic
ACTGTGGACCAAAGTCAACC+GGG 0.662686 2_1:+59198884 Msa0214640:CDS
TGAAGTCAAGAGGGCCAAGG+AGG 0.670940 2_1:+59198642 Msa0214640:CDS
GACATAGCTTATGTAGACCT+AGG 0.681895 2_1:+59196935 Msa0214640:five_prime_UTR
TGAGCCACCATAAAACAGCA+TGG 0.688795 2_1:-59196408 None:intergenic
AGGGGACGATACCTTCACCA+TGG 0.697116 2_1:+59198952 Msa0214640:CDS
GCAGCTAGAAGATATAGGTG+TGG 0.701333 2_1:+59197455 Msa0214640:CDS
TGAAGAACAAAGCTCCAAGG+TGG 0.711390 2_1:+59198780 Msa0214640:CDS
GATGCTGACGACTTATCACA+AGG 0.713430 2_1:+59197506 Msa0214640:CDS
TTGTGAAATATCGGCCATGG+TGG 0.734615 2_1:+59196383 Msa0214640:five_prime_UTR
GTGGACCAAAGTCAACCGGG+AGG 0.755821 2_1:+59198887 Msa0214640:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGAAATTTCATTAATATATA+AGG + chr2_1:59196612-59196631 Msa0214640:five_prime_UTR 10.0%
!! GAAATTTCATTAATATATAA+GGG + chr2_1:59196613-59196632 Msa0214640:five_prime_UTR 10.0%
!! AAATTTCATTAATATATAAG+GGG + chr2_1:59196614-59196633 Msa0214640:five_prime_UTR 10.0%
!! AATTTCATTAATATATAAGG+GGG + chr2_1:59196615-59196634 Msa0214640:five_prime_UTR 15.0%
!! TATACTAATTTAGTAATTGT+AGG + chr2_1:59196871-59196890 Msa0214640:five_prime_UTR 15.0%
!! ATGTTTACAGTAAATAATAT+TGG - chr2_1:59197133-59197152 None:intergenic 15.0%
!!! TTTTATTTTTTTTATGGTTG+AGG - chr2_1:59197858-59197877 None:intergenic 15.0%
!! CAACCATAAAAAAAATAAAA+AGG + chr2_1:59197858-59197877 Msa0214640:intron 15.0%
!!! GTTCCTTTTTATTTTTTTTA+TGG - chr2_1:59197864-59197883 None:intergenic 15.0%
!! ATTCACTATAGTTTAATAAA+AGG - chr2_1:59198088-59198107 None:intergenic 15.0%
!! AAAAACCATAATTGAATTTA+TGG + chr2_1:59198392-59198411 Msa0214640:intron 15.0%
!! ATTTACCATAAATTCAATTA+TGG - chr2_1:59198400-59198419 None:intergenic 15.0%
!!! ATTATTTTTGCATTTAAGCA+TGG + chr2_1:59196726-59196745 Msa0214640:five_prime_UTR 20.0%
!!! TACTTAATTCTATCATCTTT+TGG + chr2_1:59196759-59196778 Msa0214640:five_prime_UTR 20.0%
!! ACATTCTTATTATGTAAATC+TGG + chr2_1:59196844-59196863 Msa0214640:five_prime_UTR 20.0%
!! ATTTACTGTAAACATATGTT+TGG + chr2_1:59197138-59197157 Msa0214640:five_prime_UTR 20.0%
!! AATCAGCAATTTATAATTGT+CGG - chr2_1:59197306-59197325 None:intergenic 20.0%
!!! TTACATTTTCTTTGATGTTT+TGG + chr2_1:59197348-59197367 Msa0214640:exon 20.0%
!!! TACATTTTCTTTGATGTTTT+GGG + chr2_1:59197349-59197368 Msa0214640:exon 20.0%
!!! TTTTTATCCTTTTTGTTGAA+TGG + chr2_1:59197635-59197654 Msa0214640:intron 20.0%
!!! CTTTGATCTTAGATATTTTT+AGG - chr2_1:59197985-59198004 None:intergenic 20.0%
!!! CAAGAGTTTTTGTATAAATA+GGG - chr2_1:59198180-59198199 None:intergenic 20.0%
!!! TCAAGAGTTTTTGTATAAAT+AGG - chr2_1:59198181-59198200 None:intergenic 20.0%
!!! TTTTTTTTTGTCCTTTTACT+TGG + chr2_1:59199119-59199138 Msa0214640:three_prime_UTR 20.0%
!! AGCTTATCTTTTCTTATTGT+GGG - chr2_1:59196494-59196513 None:intergenic 25.0%
!! AAGCTTATCTTTTCTTATTG+TGG - chr2_1:59196495-59196514 None:intergenic 25.0%
! ATGATAGAATTAAGTAACCA+TGG - chr2_1:59196755-59196774 None:intergenic 25.0%
! TTCTTATTATGTAAATCTGG+AGG + chr2_1:59196847-59196866 Msa0214640:five_prime_UTR 25.0%
! AAATGATGATAGTTAGTACA+AGG + chr2_1:59197262-59197281 Msa0214640:five_prime_UTR 25.0%
!!! GAAACATTTTATTTCTTTCC+TGG - chr2_1:59197593-59197612 None:intergenic 25.0%
! TTAATGTAAGATACTGTCAT+TGG + chr2_1:59197665-59197684 Msa0214640:intron 25.0%
! AAAGGAACTTACTCTTATAA+AGG + chr2_1:59197876-59197895 Msa0214640:intron 25.0%
!!! GATTTTAAAAATCCAAAGCA+CGG + chr2_1:59198011-59198030 Msa0214640:intron 25.0%
! CATTATGCTTATTTATTCCT+TGG + chr2_1:59199069-59199088 Msa0214640:three_prime_UTR 25.0%
! TATTCACTTTATAACATCCA+AGG - chr2_1:59199089-59199108 None:intergenic 25.0%
! TGAGTCTATAATTTGATTGT+AGG + chr2_1:59199143-59199162 Msa0214640:three_prime_UTR 25.0%
ATGAGTAAATTGAACAAGGT+AGG - chr2_1:59196336-59196355 None:intergenic 30.0%
TAGGATGAGTAAATTGAACA+AGG - chr2_1:59196340-59196359 None:intergenic 30.0%
GGTTTAGTGTTGTGAAATAT+CGG + chr2_1:59196374-59196393 Msa0214640:five_prime_UTR 30.0%
!!! GATATTTTAGCTTTCCGTTA+CGG + chr2_1:59196439-59196458 Msa0214640:five_prime_UTR 30.0%
! AATGTTTTCAGAGATTGAAG+TGG + chr2_1:59196558-59196577 Msa0214640:five_prime_UTR 30.0%
ACAAATCAGAGTATGCATTT+AGG - chr2_1:59196692-59196711 None:intergenic 30.0%
! AAATGCATACTCTGATTTGT+TGG + chr2_1:59196692-59196711 Msa0214640:five_prime_UTR 30.0%
ACTACTACAGGAAAAGAAAA+AGG - chr2_1:59197024-59197043 None:intergenic 30.0%
AAAGGAATATTCACTACTAC+AGG - chr2_1:59197036-59197055 None:intergenic 30.0%
ATTCCTTTGTAGTAGTTATG+TGG + chr2_1:59197048-59197067 Msa0214640:five_prime_UTR 30.0%
TCCAATTCTTGAGCAATTAA+TGG + chr2_1:59197105-59197124 Msa0214640:five_prime_UTR 30.0%
TCCATTAATTGCTCAAGAAT+TGG - chr2_1:59197109-59197128 None:intergenic 30.0%
TACTGTAAACATATGTTTGG+TGG + chr2_1:59197141-59197160 Msa0214640:five_prime_UTR 30.0%
TATTAGCAGCTAGAAGATAT+AGG + chr2_1:59197450-59197469 Msa0214640:CDS 30.0%
CGATATAACATACTCAGTAT+GGG - chr2_1:59197483-59197502 None:intergenic 30.0%
ACAAAAAGTTATTTGCTTCC+AGG + chr2_1:59197572-59197591 Msa0214640:intron 30.0%
CACCAATATTATGTAGCTTT+GGG - chr2_1:59198130-59198149 None:intergenic 30.0%
TCACCAATATTATGTAGCTT+TGG - chr2_1:59198131-59198150 None:intergenic 30.0%
TTGTATAAATAGGGCTAAGA+GGG - chr2_1:59198171-59198190 None:intergenic 30.0%
TTTGTATAAATAGGGCTAAG+AGG - chr2_1:59198172-59198191 None:intergenic 30.0%
AAAACTCTTGATGTGTATGT+TGG + chr2_1:59198190-59198209 Msa0214640:intron 30.0%
AAGACAGAACAGAAACTAAA+TGG - chr2_1:59198447-59198466 None:intergenic 30.0%
CTTCCATAGTCATATATTTG+TGG + chr2_1:59199223-59199242 Msa0214640:three_prime_UTR 30.0%
AGACCACAAATATATGACTA+TGG - chr2_1:59199229-59199248 None:intergenic 30.0%
CATCCTATTCCTAACTAGTT+CGG + chr2_1:59196353-59196372 Msa0214640:five_prime_UTR 35.0%
AAACCGAACTAGTTAGGAAT+AGG - chr2_1:59196359-59196378 None:intergenic 35.0%
AACACTAAACCGAACTAGTT+AGG - chr2_1:59196365-59196384 None:intergenic 35.0%
! GTCGATTGATAACACTAGTT+TGG + chr2_1:59196465-59196484 Msa0214640:five_prime_UTR 35.0%
! TTTTCAGAGATTGAAGTGGA+AGG + chr2_1:59196562-59196581 Msa0214640:five_prime_UTR 35.0%
! TCAGCAATTTTTCTAGACCT+AGG - chr2_1:59196955-59196974 None:intergenic 35.0%
AAAATTGCTGATCCTGATAC+AGG + chr2_1:59196964-59196983 Msa0214640:five_prime_UTR 35.0%
CAACCACATAACTACTACAA+AGG - chr2_1:59197054-59197073 None:intergenic 35.0%
GAACAACTCTCTTTCAATCA+TGG - chr2_1:59197186-59197205 None:intergenic 35.0%
TTTCCTTTATCTGTGAATGC+TGG - chr2_1:59197425-59197444 None:intergenic 35.0%
GCGATATAACATACTCAGTA+TGG - chr2_1:59197484-59197503 None:intergenic 35.0%
CAAGGAAGTAATGCTTATGT+TGG + chr2_1:59197524-59197543 Msa0214640:CDS 35.0%
TCTTATGTGTTTCTTCTGCT+TGG + chr2_1:59197611-59197630 Msa0214640:intron 35.0%
TAATTCTCAGTTTCCTACCA+AGG - chr2_1:59197776-59197795 None:intergenic 35.0%
TGAGAATTAGAACTCTGTGT+TGG + chr2_1:59197787-59197806 Msa0214640:intron 35.0%
!! TATGTAGCTTTGGGTGTAAA+TGG - chr2_1:59198121-59198140 None:intergenic 35.0%
CACCCAAAGCTACATAATAT+TGG + chr2_1:59198125-59198144 Msa0214640:intron 35.0%
TGAAACACAGATTTCAGCAT+AGG - chr2_1:59198243-59198262 None:intergenic 35.0%
GAAATCTGTGTTTCATAGCT+TGG + chr2_1:59198248-59198267 Msa0214640:intron 35.0%
CTTCAAAAGGTTGAACTTGT+AGG - chr2_1:59198627-59198646 None:intergenic 35.0%
!!! TTCAACCTTTTGAAGTCAAG+AGG + chr2_1:59198632-59198651 Msa0214640:CDS 35.0%
!!! TCAACCTTTTGAAGTCAAGA+GGG + chr2_1:59198633-59198652 Msa0214640:CDS 35.0%
AAAGTTTCCTGTCTCATCAA+TGG + chr2_1:59198679-59198698 Msa0214640:CDS 35.0%
AAGTTTCCTGTCTCATCAAT+GGG + chr2_1:59198680-59198699 Msa0214640:CDS 35.0%
! TCAGATAGCAAGTCTTTTGA+TGG + chr2_1:59198710-59198729 Msa0214640:CDS 35.0%
TATAGACTCAGCCAAGTAAA+AGG - chr2_1:59199133-59199152 None:intergenic 35.0%
! GACTTGCTTCAGTCAATGTA+TGG + chr2_1:59196306-59196325 Msa0214640:five_prime_UTR 40.0%
TTTATGGTGGCTCAAGATGA+TGG + chr2_1:59196417-59196436 Msa0214640:five_prime_UTR 40.0%
TATCAATCGACGAACCGTAA+CGG - chr2_1:59196456-59196475 None:intergenic 40.0%
GACATAGCTTATGTAGACCT+AGG + chr2_1:59196935-59196954 Msa0214640:five_prime_UTR 40.0%
GTTCGTAACTGTGTGATTTG+TGG + chr2_1:59197202-59197221 Msa0214640:five_prime_UTR 40.0%
ATGCTTATGTTGGCAAGCTA+AGG + chr2_1:59197534-59197553 Msa0214640:CDS 40.0%
! ATCCTTTTTGTTGAATGGCG+TGG + chr2_1:59197640-59197659 Msa0214640:intron 40.0%
! TGAAAACCTTCACTTGTTGC+AGG + chr2_1:59197911-59197930 Msa0214640:intron 40.0%
! GAAAACCTTCACTTGTTGCA+GGG + chr2_1:59197912-59197931 Msa0214640:intron 40.0%
! AAAACCTTCACTTGTTGCAG+GGG + chr2_1:59197913-59197932 Msa0214640:intron 40.0%
GCTGTCGTAAATTGTGAAGT+TGG - chr2_1:59198492-59198511 None:intergenic 40.0%
! TGATGGTTTGGCACCATTAT+GGG - chr2_1:59198522-59198541 None:intergenic 40.0%
! ATGATGGTTTGGCACCATTA+TGG - chr2_1:59198523-59198542 None:intergenic 40.0%
TCTTGAAGAACAAAGCTCCA+AGG + chr2_1:59198777-59198796 Msa0214640:CDS 40.0%
ATCTGTGAAGAACTTTCAGC+TGG + chr2_1:59198856-59198875 Msa0214640:CDS 40.0%
AGGGAAAGGAGATGAAGAAA+CGG + chr2_1:59198907-59198926 Msa0214640:CDS 40.0%
TCCTCCAGTTTGGTAAAGTA+GGG + chr2_1:59198933-59198952 Msa0214640:CDS 40.0%
! ATACCTTCACCATGGACTAT+AGG + chr2_1:59198960-59198979 Msa0214640:CDS 40.0%
GTTTAGTGCCAAAGCTAGTT+AGG - chr2_1:59199021-59199040 None:intergenic 40.0%
!! AACTAGCTTTGGCACTAAAC+TGG + chr2_1:59199021-59199040 Msa0214640:CDS 40.0%
GTGTTGTGAAATATCGGCCA+TGG + chr2_1:59196380-59196399 Msa0214640:five_prime_UTR 45.0%
TTGTGAAATATCGGCCATGG+TGG + chr2_1:59196383-59196402 Msa0214640:five_prime_UTR 45.0%
TGAGCCACCATAAAACAGCA+TGG - chr2_1:59196411-59196430 None:intergenic 45.0%
TAAGGGGGAGATTTCATGAG+TGG + chr2_1:59196630-59196649 Msa0214640:five_prime_UTR 45.0%
GGAGATTTCATGAGTGGCTA+AGG + chr2_1:59196636-59196655 Msa0214640:five_prime_UTR 45.0%
GCATTTAAGCATGGTGTCCA+TGG + chr2_1:59196735-59196754 Msa0214640:five_prime_UTR 45.0%
ATGAGCAGAACAGGAAAGAG+GGG - chr2_1:59197400-59197419 None:intergenic 45.0%
GATGAGCAGAACAGGAAAGA+GGG - chr2_1:59197401-59197420 None:intergenic 45.0%
TCGCCAGCATTCACAGATAA+AGG + chr2_1:59197419-59197438 Msa0214640:CDS 45.0%
GCAGCTAGAAGATATAGGTG+TGG + chr2_1:59197455-59197474 Msa0214640:CDS 45.0%
GATGCTGACGACTTATCACA+AGG + chr2_1:59197506-59197525 Msa0214640:CDS 45.0%
CACCACGCCATTCAACAAAA+AGG - chr2_1:59197645-59197664 None:intergenic 45.0%
TTTCTCTGTTGTGATCGCCT+TGG + chr2_1:59197756-59197775 Msa0214640:intron 45.0%
! AACTCTGTGTTGGCACAATC+AGG + chr2_1:59197797-59197816 Msa0214640:intron 45.0%
! ACTCTGTGTTGGCACAATCA+GGG + chr2_1:59197798-59197817 Msa0214640:intron 45.0%
! GGTGCAAACAGGATGGATTT+CGG + chr2_1:59197934-59197953 Msa0214640:intron 45.0%
!! GTAGCTTTGGGTGTAAATGG+AGG - chr2_1:59198118-59198137 None:intergenic 45.0%
TCTGTTCTGTCTTCACAGCT+CGG + chr2_1:59198454-59198473 Msa0214640:intron 45.0%
! GATGGTTTGGCACCATTATG+GGG - chr2_1:59198521-59198540 None:intergenic 45.0%
! TCTGTTTGCTGGCAAAACGT+CGG - chr2_1:59198559-59198578 None:intergenic 45.0%
TTGAGGGCTTATCTGTTTGC+TGG - chr2_1:59198570-59198589 None:intergenic 45.0%
AGTTACCAGCAGGAACTTGA+GGG - chr2_1:59198586-59198605 None:intergenic 45.0%
AAGTTACCAGCAGGAACTTG+AGG - chr2_1:59198587-59198606 None:intergenic 45.0%
TTGGCCCTCTTGACTTCAAA+AGG - chr2_1:59198640-59198659 None:intergenic 45.0%
!!! TTTTGAAGTCAAGAGGGCCA+AGG + chr2_1:59198639-59198658 Msa0214640:CDS 45.0%
CTTTCACCCATTGATGAGAC+AGG - chr2_1:59198689-59198708 None:intergenic 45.0%
TGAAGAACAAAGCTCCAAGG+TGG + chr2_1:59198780-59198799 Msa0214640:CDS 45.0%
TACTGTGGACCAAAGTCAAC+CGG + chr2_1:59198883-59198902 Msa0214640:CDS 45.0%
CTCCTCCAGTTTGGTAAAGT+AGG + chr2_1:59198932-59198951 Msa0214640:CDS 45.0%
CCTCCAGTTTGGTAAAGTAG+GGG + chr2_1:59198934-59198953 Msa0214640:CDS 45.0%
! GCCATTTGCCTAACTAGCTT+TGG + chr2_1:59199010-59199029 Msa0214640:CDS 45.0%
GCCAAAGCTAGTTAGGCAAA+TGG - chr2_1:59199014-59199033 None:intergenic 45.0%
!! AAAACAGCATGGTGCCACCA+TGG - chr2_1:59196400-59196419 None:intergenic 50.0%
!!! GGTGGCACCATGCTGTTTTA+TGG + chr2_1:59196401-59196420 Msa0214640:five_prime_UTR 50.0%
!!! GGCACCATGCTGTTTTATGG+TGG + chr2_1:59196404-59196423 Msa0214640:five_prime_UTR 50.0%
ACTGACTGCAAGCCTGTATC+AGG - chr2_1:59196979-59196998 None:intergenic 50.0%
CGATGAGCAGAACAGGAAAG+AGG - chr2_1:59197402-59197421 None:intergenic 50.0%
TCTGTTGTGATCGCCTTGGT+AGG + chr2_1:59197760-59197779 Msa0214640:intron 50.0%
CTTCACAGCTCGGACTTTCT+TGG + chr2_1:59198464-59198483 Msa0214640:intron 50.0%
! AGCTCTGGCACATGATGGTT+TGG - chr2_1:59198534-59198553 None:intergenic 50.0%
ATAAGCCCTCAAGTTCCTGC+TGG + chr2_1:59198578-59198597 Msa0214640:CDS 50.0%
AGTTCCTGCTGGTAACTTCG+AGG + chr2_1:59198589-59198608 Msa0214640:CDS 50.0%
AGTGAGCACATCATCCTCCT+TGG - chr2_1:59198659-59198678 None:intergenic 50.0%
TGCTGGTGCTTGAACTTCCA+TGG + chr2_1:59198818-59198837 Msa0214640:CDS 50.0%
CTTTCAGCTGGTTGCTACTG+TGG + chr2_1:59198868-59198887 Msa0214640:CDS 50.0%
ACTGTGGACCAAAGTCAACC+GGG + chr2_1:59198884-59198903 Msa0214640:CDS 50.0%
TCTTCATCTCCTTTCCCTCC+CGG - chr2_1:59198905-59198924 None:intergenic 50.0%
GAAACGGTTCTCCTCCAGTT+TGG + chr2_1:59198923-59198942 Msa0214640:CDS 50.0%
CCCCTACTTTACCAAACTGG+AGG - chr2_1:59198937-59198956 None:intergenic 50.0%
CGTCCCCTACTTTACCAAAC+TGG - chr2_1:59198940-59198959 None:intergenic 50.0%
CTGCCTATAGTCCATGGTGA+AGG - chr2_1:59198966-59198985 None:intergenic 50.0%
TTAGGCAAATGGCAAACGCC+TGG - chr2_1:59199003-59199022 None:intergenic 50.0%
AGAACAGGAAAGAGGGGCAG+GGG - chr2_1:59197394-59197413 None:intergenic 55.0%
CAGAACAGGAAAGAGGGGCA+GGG - chr2_1:59197395-59197414 None:intergenic 55.0%
ATGCTGGCGATGAGCAGAAC+AGG - chr2_1:59197409-59197428 None:intergenic 55.0%
TGCACCCCTGCAACAAGTGA+AGG - chr2_1:59197920-59197939 None:intergenic 55.0%
! CTTGTTGCAGGGGTGCAAAC+AGG + chr2_1:59197923-59197942 Msa0214640:intron 55.0%
! TTGCAGGGGTGCAAACAGGA+TGG + chr2_1:59197927-59197946 Msa0214640:intron 55.0%
GGTTTGGCACCATTATGGGG+TGG - chr2_1:59198518-59198537 None:intergenic 55.0%
CTGCTGGTAACTTCGAGGCA+GGG + chr2_1:59198594-59198613 Msa0214640:CDS 55.0%
TGCTGGTAACTTCGAGGCAG+GGG + chr2_1:59198595-59198614 Msa0214640:CDS 55.0%
TGAAGTCAAGAGGGCCAAGG+AGG + chr2_1:59198642-59198661 Msa0214640:CDS 55.0%
TGCAAATGCTCGTGCCACCT+TGG - chr2_1:59198797-59198816 None:intergenic 55.0%
CTTCCATGGTCGTGTGACAG+TGG + chr2_1:59198832-59198851 Msa0214640:CDS 55.0%
ATGCCACTGTCACACGACCA+TGG - chr2_1:59198838-59198857 None:intergenic 55.0%
TGGACCAAAGTCAACCGGGA+GGG + chr2_1:59198888-59198907 Msa0214640:CDS 55.0%
! CTTTCCCTCCCGGTTGACTT+TGG - chr2_1:59198895-59198914 None:intergenic 55.0%
CAAAGTCAACCGGGAGGGAA+AGG + chr2_1:59198893-59198912 Msa0214640:CDS 55.0%
AGGGGACGATACCTTCACCA+TGG + chr2_1:59198952-59198971 Msa0214640:CDS 55.0%
CAAGGGCTGCCTATAGTCCA+TGG - chr2_1:59198972-59198991 None:intergenic 55.0%
AATGGCAAACGCCTGGAAGG+CGG - chr2_1:59198996-59199015 None:intergenic 55.0%
GCAAATGGCAAACGCCTGGA+AGG - chr2_1:59198999-59199018 None:intergenic 55.0%
GCAGAACAGGAAAGAGGGGC+AGG - chr2_1:59197396-59197415 None:intergenic 60.0%
TGCAGTGCAGTGCCGTGCTT+TGG - chr2_1:59198026-59198045 None:intergenic 60.0%
GACAGCCAGCCACCCCATAA+TGG + chr2_1:59198506-59198525 Msa0214640:CDS 60.0%
TGGCACCATTATGGGGTGGC+TGG - chr2_1:59198514-59198533 None:intergenic 60.0%
!! CGGCTAGCTCTGGCACATGA+TGG - chr2_1:59198539-59198558 None:intergenic 60.0%
! GGCAAAACGTCGGCTAGCTC+TGG - chr2_1:59198549-59198568 None:intergenic 60.0%
CCTGCCTCGAAGTTACCAGC+AGG - chr2_1:59198596-59198615 None:intergenic 60.0%
CCTGCTGGTAACTTCGAGGC+AGG + chr2_1:59198593-59198612 Msa0214640:CDS 60.0%
GGCACGAGCATTTGCAGTGC+TGG + chr2_1:59198801-59198820 Msa0214640:CDS 60.0%
GTGGACCAAAGTCAACCGGG+AGG + chr2_1:59198887-59198906 Msa0214640:CDS 60.0%
AACGCCTGGAAGGCGGACAA+GGG - chr2_1:59198989-59199008 None:intergenic 60.0%
AAACGCCTGGAAGGCGGACA+AGG - chr2_1:59198990-59199009 None:intergenic 60.0%
GCAGCCCTTGTCCGCCTTCC+AGG + chr2_1:59198982-59199001 Msa0214640:CDS 70.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 59196306 59199250 59196306 ID=Msa0214640;Name=Msa0214640
chr2_1 mRNA 59196306 59199250 59196306 ID=Msa0214640-mRNA-1;Parent=Msa0214640;Name=Msa0214640-mRNA-1;_AED=0.23;_eAED=0.23;_QI=1056|1|1|1|1|1|2|196|258
chr2_1 exon 59196306 59197555 59196306 ID=Msa0214640-mRNA-1:exon:16297;Parent=Msa0214640-mRNA-1
chr2_1 exon 59198472 59199250 59198472 ID=Msa0214640-mRNA-1:exon:16298;Parent=Msa0214640-mRNA-1
chr2_1 five_prime_UTR 59196306 59197361 59196306 ID=Msa0214640-mRNA-1:five_prime_utr;Parent=Msa0214640-mRNA-1
chr2_1 CDS 59197362 59197555 59197362 ID=Msa0214640-mRNA-1:cds;Parent=Msa0214640-mRNA-1
chr2_1 CDS 59198472 59199054 59198472 ID=Msa0214640-mRNA-1:cds;Parent=Msa0214640-mRNA-1
chr2_1 three_prime_UTR 59199055 59199250 59199055 ID=Msa0214640-mRNA-1:three_prime_utr;Parent=Msa0214640-mRNA-1
Gene Sequence

>Msa0214640

ATGTTTTGGGAGTTGATGCTTCTTTTATTCCCCTGCCCCTCTTTCCTGTTCTGCTCATCGCCAGCATTCACAGATAAAGGAAAGTTTCTATTAGCAGCTAGAAGATATAGGTGTGGTACCCATACTGAGTATGTTATATCGCTTGATGCTGACGACTTATCACAAGGAAGTAATGCTTATGTTGGCAAGCTAAGCTCGGACTTTCTTGGCACCAACTTCACAATTTACGACAGCCAGCCACCCCATAATGGTGCCAAACCATCATGTGCCAGAGCTAGCCGACGTTTTGCCAGCAAACAGATAAGCCCTCAAGTTCCTGCTGGTAACTTCGAGGCAGGGGATGTTTCCTACAAGTTCAACCTTTTGAAGTCAAGAGGGCCAAGGAGGATGATGTGCTCACTAAAGTTTCCTGTCTCATCAATGGGTGAAAGTTCAGATAGCAAGTCTTTTGATGGTCAAAAGATGCACAGTAAAGAACATGCTGCTTCTGCGCACACAATCTTGAAGAACAAAGCTCCAAGGTGGCACGAGCATTTGCAGTGCTGGTGCTTGAACTTCCATGGTCGTGTGACAGTGGCATCTGTGAAGAACTTTCAGCTGGTTGCTACTGTGGACCAAAGTCAACCGGGAGGGAAAGGAGATGAAGAAACGGTTCTCCTCCAGTTTGGTAAAGTAGGGGACGATACCTTCACCATGGACTATAGGCAGCCCTTGTCCGCCTTCCAGGCGTTTGCCATTTGCCTAACTAGCTTTGGCACTAAACTGGCTTGTGAGTAA
Protein sequence

>Msa0214640

MFWELMLLLFPCPSFLFCSSPAFTDKGKFLLAARRYRCGTHTEYVISLDADDLSQGSNAYVGKLSSDFLGTNFTIYDSQPPHNGAKPSCARASRRFASKQISPQVPAGNFEAGDVSYKFNLLKSRGPRRMMCSLKFPVSSMGESSDSKSFDGQKMHSKEHAASAHTILKNKAPRWHEHLQCWCLNFHGRVTVASVKNFQLVATVDQSQPGGKGDEETVLLQFGKVGDDTFTMDYRQPLSAFQAFAICLTSFGTKLACE*