Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0215790 | XP_013464663.1 | 92.437 | 119 | 6 | 1 | 1 | 119 | 1 | 116 | 1.29e-72 | 222 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0215790 | A0A072VB72 | 92.437 | 119 | 6 | 1 | 1 | 119 | 1 | 116 | 6.15e-73 | 222 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0215790 | Msa0333630 | 0.998652 | 8.791660e-272 | -8.615850e-47 |
| Msa0215790 | Msa0928010 | 0.805855 | 1.091037e-49 | -8.615850e-47 |
| Msa0215790 | Msa0998700 | 0.812570 | 3.966499e-51 | -8.615850e-47 |
| Msa0215790 | Msa1340180 | 0.990891 | 7.751091e-185 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0215790 | MtrunA17_Chr2g0317051 | 92.437 | 119 | 6 | 1 | 1 | 119 | 1 | 116 | 1.18e-76 | 222 |
| Msa0215790 | MtrunA17_Chr1g0179711 | 93.939 | 99 | 6 | 0 | 1 | 99 | 1 | 99 | 3.19e-63 | 187 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 24 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTTGATTTATTGCTCCTAA+TGG | 0.262425 | 2_1:-61205149 | None:intergenic |
| AGAAGTAGTGAAGATTTATC+AGG | 0.364128 | 2_1:+61205326 | Msa0215790:CDS |
| AACAAAGAAAACTGTACAAT+TGG | 0.403825 | 2_1:+61205247 | Msa0215790:CDS |
| GTCTTTGCACTGAGCAAGAA+AGG | 0.411430 | 2_1:-61204929 | None:intergenic |
| ATTCAGGGTGGTGATCCATT+AGG | 0.431783 | 2_1:+61205134 | Msa0215790:intron |
| GTTGATAACTTTAGCAGCAT+TGG | 0.452113 | 2_1:-61204968 | None:intergenic |
| CTAGAGAGTGTTGAAGAAGT+TGG | 0.457188 | 2_1:+61205194 | Msa0215790:CDS |
| GAGTGTTGAAGAAGTTGGTA+AGG | 0.463197 | 2_1:+61205199 | Msa0215790:CDS |
| TCTTGCTCAGTGCAAAGACT+TGG | 0.489170 | 2_1:+61204933 | Msa0215790:CDS |
| AACTTGGAGATGGCTGTGAT+AGG | 0.503601 | 2_1:+61205351 | Msa0215790:CDS |
| AACTTTAGCAGCATTGGTAG+TGG | 0.505111 | 2_1:-61204962 | None:intergenic |
| CCATTAGGAGCAATAAATCA+AGG | 0.506320 | 2_1:+61205149 | Msa0215790:CDS |
| GAAGATTTATCAGGGCAACT+TGG | 0.516603 | 2_1:+61205335 | Msa0215790:CDS |
| AGGAAAATGTGGAAACTGCA+TGG | 0.519693 | 2_1:+61205219 | Msa0215790:CDS |
| TTATCAGGGCAACTTGGAGA+TGG | 0.537867 | 2_1:+61205341 | Msa0215790:CDS |
| AGAAGTTGGTAAGGAAAATG+TGG | 0.552960 | 2_1:+61205208 | Msa0215790:CDS |
| GAAGTAGTGAAGATTTATCA+GGG | 0.567179 | 2_1:+61205327 | Msa0215790:CDS |
| TCTTTGCACTGAGCAAGAAA+GGG | 0.570085 | 2_1:-61204928 | None:intergenic |
| AGGGTCAGAGAAAGCAAGAG+AGG | 0.619602 | 2_1:+61205169 | Msa0215790:CDS |
| GACTGAGACAACCACTGCTG+AGG | 0.629251 | 2_1:+61205271 | Msa0215790:CDS |
| TGAGGCTGATATGAATGTTG+TGG | 0.639431 | 2_1:+61205289 | Msa0215790:CDS |
| CATTAGGAGCAATAAATCAA+GGG | 0.647640 | 2_1:+61205150 | Msa0215790:CDS |
| ATTCATATCAGCCTCAGCAG+TGG | 0.674496 | 2_1:-61205282 | None:intergenic |
| GGAAAATGTGGAAACTGCAT+GGG | 0.686615 | 2_1:+61205220 | Msa0215790:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTGTGAAAGAAAAAAAATTG+AGG | - | chr2_1:61205035-61205054 | None:intergenic | 20.0% |
| !!! | TTTTTTTTTTAAATTCAGGG+TGG | + | chr2_1:61205122-61205141 | Msa0215790:intron | 20.0% |
| ! | AACAAAGAAAACTGTACAAT+TGG | + | chr2_1:61205247-61205266 | Msa0215790:CDS | 25.0% |
| CATTAGGAGCAATAAATCAA+GGG | + | chr2_1:61205150-61205169 | Msa0215790:CDS | 30.0% | |
| AGAAGTAGTGAAGATTTATC+AGG | + | chr2_1:61205326-61205345 | Msa0215790:CDS | 30.0% | |
| GAAGTAGTGAAGATTTATCA+GGG | + | chr2_1:61205327-61205346 | Msa0215790:CDS | 30.0% | |
| !! | GTTGATAACTTTAGCAGCAT+TGG | - | chr2_1:61204971-61204990 | None:intergenic | 35.0% |
| CCTTGATTTATTGCTCCTAA+TGG | - | chr2_1:61205152-61205171 | None:intergenic | 35.0% | |
| CCATTAGGAGCAATAAATCA+AGG | + | chr2_1:61205149-61205168 | Msa0215790:CDS | 35.0% | |
| !! | AGAAGTTGGTAAGGAAAATG+TGG | + | chr2_1:61205208-61205227 | Msa0215790:CDS | 35.0% |
| TCTTTGCACTGAGCAAGAAA+GGG | - | chr2_1:61204931-61204950 | None:intergenic | 40.0% | |
| !! | AACTTTAGCAGCATTGGTAG+TGG | - | chr2_1:61204965-61204984 | None:intergenic | 40.0% |
| !! | CTAGAGAGTGTTGAAGAAGT+TGG | + | chr2_1:61205194-61205213 | Msa0215790:CDS | 40.0% |
| !! | GAGTGTTGAAGAAGTTGGTA+AGG | + | chr2_1:61205199-61205218 | Msa0215790:CDS | 40.0% |
| AGGAAAATGTGGAAACTGCA+TGG | + | chr2_1:61205219-61205238 | Msa0215790:CDS | 40.0% | |
| GGAAAATGTGGAAACTGCAT+GGG | + | chr2_1:61205220-61205239 | Msa0215790:CDS | 40.0% | |
| TGAGGCTGATATGAATGTTG+TGG | + | chr2_1:61205289-61205308 | Msa0215790:CDS | 40.0% | |
| GAAGATTTATCAGGGCAACT+TGG | + | chr2_1:61205335-61205354 | Msa0215790:CDS | 40.0% | |
| GTCTTTGCACTGAGCAAGAA+AGG | - | chr2_1:61204932-61204951 | None:intergenic | 45.0% | |
| TCTTGCTCAGTGCAAAGACT+TGG | + | chr2_1:61204933-61204952 | Msa0215790:CDS | 45.0% | |
| ATTCAGGGTGGTGATCCATT+AGG | + | chr2_1:61205134-61205153 | Msa0215790:intron | 45.0% | |
| ATTCATATCAGCCTCAGCAG+TGG | - | chr2_1:61205285-61205304 | None:intergenic | 45.0% | |
| TTATCAGGGCAACTTGGAGA+TGG | + | chr2_1:61205341-61205360 | Msa0215790:CDS | 45.0% | |
| AACTTGGAGATGGCTGTGAT+AGG | + | chr2_1:61205351-61205370 | Msa0215790:CDS | 45.0% | |
| !!! | TTTTTTTTTTTTTTAAATTC+AGG | + | chr2_1:61205118-61205137 | Msa0215790:intron | 5.0% |
| !!! | TTTTTTTTTTTTTAAATTCA+GGG | + | chr2_1:61205119-61205138 | Msa0215790:intron | 5.0% |
| AGGGTCAGAGAAAGCAAGAG+AGG | + | chr2_1:61205169-61205188 | Msa0215790:CDS | 50.0% | |
| GACTGAGACAACCACTGCTG+AGG | + | chr2_1:61205271-61205290 | Msa0215790:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_1 | gene | 61204913 | 61205385 | 61204913 | ID=Msa0215790;Name=Msa0215790 |
| chr2_1 | mRNA | 61204913 | 61205385 | 61204913 | ID=Msa0215790-mRNA-1;Parent=Msa0215790;Name=Msa0215790-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|0|0|1|1|1|2|0|119 |
| chr2_1 | exon | 61204913 | 61205026 | 61204913 | ID=Msa0215790-mRNA-1:exon:16897;Parent=Msa0215790-mRNA-1 |
| chr2_1 | exon | 61205140 | 61205385 | 61205140 | ID=Msa0215790-mRNA-1:exon:16898;Parent=Msa0215790-mRNA-1 |
| chr2_1 | CDS | 61204913 | 61205026 | 61204913 | ID=Msa0215790-mRNA-1:cds;Parent=Msa0215790-mRNA-1 |
| chr2_1 | CDS | 61205140 | 61205385 | 61205140 | ID=Msa0215790-mRNA-1:cds;Parent=Msa0215790-mRNA-1 |
| Gene Sequence |
| Protein sequence |
