AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0232530

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0232530 MtrunA17_Chr2g0279651 96.552 87 3 0 1 87 1 87 1.39e-58 174
Msa0232530 MtrunA17_Chr4g0049791 84.615 78 12 0 1 78 1 78 1.82e-38 123
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0232530 AT4G33640.1 76.923 78 18 0 1 78 1 78 4.22e-40 127
Msa0232530 AT4G33640.2 76.923 78 18 0 1 78 67 144 1.34e-39 129

Find 29 sgRNAs with CRISPR-Local

Find 194 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTATTCAACGTCATTTAATA+TGG 0.239487 2_2:+4817974 Msa0232530:three_prime_UTR
GTTTCGAAATCTATAACTTT+AGG 0.262910 2_2:-4818069 None:intergenic
TATAAAACAAACACCCATAA+AGG 0.380566 2_2:-4817927 None:intergenic
TGTATTGTGAAGGTTACATT+TGG 0.382119 2_2:+4817682 Msa0232530:intron
GCCTCTTGATTTCTACCTTT+AGG 0.396207 2_2:-4816176 None:intergenic
AATATATTTGAAGCACTTGT+TGG 0.411547 2_2:+4817733 Msa0232530:CDS
GTTCAGTTGGAAGCAGCAGC+AGG 0.447332 2_2:-4817856 None:intergenic
ATATATTTGAAGCACTTGTT+GGG 0.466568 2_2:+4817734 Msa0232530:CDS
TTGGGTAGATAGTGTTCAGT+TGG 0.483464 2_2:-4817869 None:intergenic
CGAAAAGTATTAACATATGA+AGG 0.490626 2_2:+4817778 Msa0232530:CDS
TACAAATACAGCAAAACTGT+TGG 0.506470 2_2:-4818091 None:intergenic
AGAAGAGGTTGGACGCCTAA+AGG 0.519063 2_2:+4816161 Msa0232530:CDS
CTATAAAAGAAGTGTGTGAA+AGG 0.528769 2_2:+4817894 Msa0232530:three_prime_UTR
GAGTGATTTCCACATTGTCA+TGG 0.529569 2_2:-4817822 None:intergenic
TCAATCAGATGGTTCTTACA+AGG 0.529642 2_2:+4816209 Msa0232530:CDS
CTTCAAATATATTTGCACAT+TGG 0.530146 2_2:-4817723 None:intergenic
AAGGTAGAAATCAAGAGGCT+TGG 0.545059 2_2:+4816180 Msa0232530:CDS
CTTGGCAAGCTTCAATCAGA+TGG 0.547560 2_2:+4816198 Msa0232530:CDS
TTCAGTTGGAAGCAGCAGCA+GGG 0.553467 2_2:-4817855 None:intergenic
GTTGGAAGCAGCAGCAGGGG+TGG 0.553565 2_2:-4817851 None:intergenic
TTGGAAGCAGCAGCAGGGGT+GGG 0.565500 2_2:-4817850 None:intergenic
AAGCATGAACGTGGAAGAAG+AGG 0.576730 2_2:+4816146 Msa0232530:exon
TATCTGAGAAGACAACAAGT+TGG 0.597136 2_2:+4816114 Msa0232530:five_prime_UTR
GCCTAAAGGTAGAAATCAAG+AGG 0.618558 2_2:+4816175 Msa0232530:CDS
ATGAACGTGGAAGAAGAGGT+TGG 0.620804 2_2:+4816150 Msa0232530:CDS
TCAGTTGGAAGCAGCAGCAG+GGG 0.624373 2_2:-4817854 None:intergenic
GAAGGTGAATTGCTGCTGCA+AGG 0.659800 2_2:+4817796 Msa0232530:CDS
AAGCAGAAGAAGCATGAACG+TGG 0.660179 2_2:+4816137 Msa0232530:exon
GCAAGGAGTCCATGACAATG+TGG 0.727047 2_2:+4817813 Msa0232530:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAATAAAATAAATAATA+AGG - chr2_2:4817550-4817569 None:intergenic 0.0%
!! AAAAAATAAAATAAATAATA+AGG - chr2_2:4817550-4817569 None:intergenic 0.0%
!!! TTATATATAGTTATTTTGAA+AGG - chr2_2:4816426-4816445 None:intergenic 10.0%
!!! TTATATATAGTTATTTTGAA+AGG - chr2_2:4816426-4816445 None:intergenic 10.0%
!!! AAATTTTAGAATTTATCACT+TGG + chr2_2:4816530-4816549 Msa0232530:intron 15.0%
!! AAGTTATAAATAGTTTCAAT+TGG - chr2_2:4817246-4817265 None:intergenic 15.0%
!! ATTGAAACTATTTATAACTT+TGG + chr2_2:4817246-4817265 Msa0232530:intron 15.0%
!! TTGAAACTATTTATAACTTT+GGG + chr2_2:4817247-4817266 Msa0232530:intron 15.0%
!!! ATAACATTTTCACTTAAAAT+TGG + chr2_2:4817321-4817340 Msa0232530:intron 15.0%
!!! AAATTTTAGAATTTATCACT+TGG + chr2_2:4816530-4816549 Msa0232530:intron 15.0%
!! AAGTTATAAATAGTTTCAAT+TGG - chr2_2:4817246-4817265 None:intergenic 15.0%
!! ATTGAAACTATTTATAACTT+TGG + chr2_2:4817246-4817265 Msa0232530:intron 15.0%
!! TTGAAACTATTTATAACTTT+GGG + chr2_2:4817247-4817266 Msa0232530:intron 15.0%
!!! ATAACATTTTCACTTAAAAT+TGG + chr2_2:4817321-4817340 Msa0232530:intron 15.0%
!! TGTATTATATTCTTAGTTCA+TGG + chr2_2:4816400-4816419 Msa0232530:intron 20.0%
!! GATAATATGTGATTATAAGA+TGG - chr2_2:4816688-4816707 None:intergenic 20.0%
!! TTTAAATGTCTTTGTTAAGA+CGG - chr2_2:4816737-4816756 None:intergenic 20.0%
!! AGACATTTAAATTAAACACA+TGG + chr2_2:4816746-4816765 Msa0232530:intron 20.0%
!!! TAGTTTTTGAATCTTGATTT+AGG - chr2_2:4816837-4816856 None:intergenic 20.0%
!! TTATAAATAGTTTCAATTGG+TGG - chr2_2:4817243-4817262 None:intergenic 20.0%
!! TGAAACTATTTATAACTTTG+GGG + chr2_2:4817248-4817267 Msa0232530:intron 20.0%
!! TAAGTGAAAATGTTATGTAT+TGG - chr2_2:4817317-4817336 None:intergenic 20.0%
!! TCTGTCTATATACATAAATT+AGG + chr2_2:4817446-4817465 Msa0232530:intron 20.0%
!! CTGTCTATATACATAAATTA+GGG + chr2_2:4817447-4817466 Msa0232530:intron 20.0%
!! AAGGATAAAAATAACCTTTA+TGG + chr2_2:4817913-4817932 Msa0232530:three_prime_UTR 20.0%
!! AGGATAAAAATAACCTTTAT+GGG + chr2_2:4817914-4817933 Msa0232530:three_prime_UTR 20.0%
!! TGTATTATATTCTTAGTTCA+TGG + chr2_2:4816400-4816419 Msa0232530:intron 20.0%
!! GATAATATGTGATTATAAGA+TGG - chr2_2:4816688-4816707 None:intergenic 20.0%
!! TTTAAATGTCTTTGTTAAGA+CGG - chr2_2:4816737-4816756 None:intergenic 20.0%
!! AGACATTTAAATTAAACACA+TGG + chr2_2:4816746-4816765 Msa0232530:intron 20.0%
!!! TAGTTTTTGAATCTTGATTT+AGG - chr2_2:4816837-4816856 None:intergenic 20.0%
!! TTATAAATAGTTTCAATTGG+TGG - chr2_2:4817243-4817262 None:intergenic 20.0%
!! TGAAACTATTTATAACTTTG+GGG + chr2_2:4817248-4817267 Msa0232530:intron 20.0%
!! TAAGTGAAAATGTTATGTAT+TGG - chr2_2:4817317-4817336 None:intergenic 20.0%
!! TCTGTCTATATACATAAATT+AGG + chr2_2:4817446-4817465 Msa0232530:intron 20.0%
!! CTGTCTATATACATAAATTA+GGG + chr2_2:4817447-4817466 Msa0232530:intron 20.0%
!! AAGGATAAAAATAACCTTTA+TGG + chr2_2:4817913-4817932 Msa0232530:three_prime_UTR 20.0%
!! AGGATAAAAATAACCTTTAT+GGG + chr2_2:4817914-4817933 Msa0232530:three_prime_UTR 20.0%
!!! TGTGTGTGTGTTTTAAATTA+CGG + chr2_2:4816077-4816096 Msa0232530:five_prime_UTR 25.0%
!! TTCAATTTTCAGATCTGTAT+AGG + chr2_2:4816267-4816286 Msa0232530:intron 25.0%
!! TCAATTTTCAGATCTGTATA+GGG + chr2_2:4816268-4816287 Msa0232530:intron 25.0%
! ATCTATCACATAAAGCAAAT+TGG - chr2_2:4816298-4816317 None:intergenic 25.0%
! GTGATGTTATTGATGTATAA+AGG - chr2_2:4816666-4816685 None:intergenic 25.0%
! CTATTAACTCTGATATTTCT+AGG - chr2_2:4816805-4816824 None:intergenic 25.0%
! GAAATATCAGAGTTAATAGA+TGG + chr2_2:4816806-4816825 Msa0232530:intron 25.0%
!!! TTTGTGATTTGAAGTTTTAG+TGG - chr2_2:4816864-4816883 None:intergenic 25.0%
!! TAGCTAAACTCAAATTTGTT+GGG - chr2_2:4817001-4817020 None:intergenic 25.0%
! TAAACAAATTCAGTCTGAAT+TGG - chr2_2:4817093-4817112 None:intergenic 25.0%
!! TTTTGTGAAGATTAAAGTGA+AGG - chr2_2:4817179-4817198 None:intergenic 25.0%
!! GGTACATTTTGAATGTAATT+TGG - chr2_2:4817293-4817312 None:intergenic 25.0%
! CTTCAAATATATTTGCACAT+TGG - chr2_2:4817726-4817745 None:intergenic 25.0%
!! AATATATTTGAAGCACTTGT+TGG + chr2_2:4817733-4817752 Msa0232530:CDS 25.0%
!! ATATATTTGAAGCACTTGTT+GGG + chr2_2:4817734-4817753 Msa0232530:CDS 25.0%
!!! CGAAAAGTATTAACATATGA+AGG + chr2_2:4817778-4817797 Msa0232530:CDS 25.0%
!! CACACTTCTTTTATAGTTTT+GGG - chr2_2:4817890-4817909 None:intergenic 25.0%
!! ACACACTTCTTTTATAGTTT+TGG - chr2_2:4817891-4817910 None:intergenic 25.0%
! TATAAAACAAACACCCATAA+AGG - chr2_2:4817930-4817949 None:intergenic 25.0%
! CTATTCAACGTCATTTAATA+TGG + chr2_2:4817974-4817993 Msa0232530:three_prime_UTR 25.0%
!!! TTTTCTTGATGACAAGATTT+TGG - chr2_2:4818018-4818037 None:intergenic 25.0%
!!! CACAATTTTCTTTTTTTGAG+AGG - chr2_2:4818048-4818067 None:intergenic 25.0%
! GTTTCGAAATCTATAACTTT+AGG - chr2_2:4818072-4818091 None:intergenic 25.0%
!!! TGTGTGTGTGTTTTAAATTA+CGG + chr2_2:4816077-4816096 Msa0232530:five_prime_UTR 25.0%
!! TTCAATTTTCAGATCTGTAT+AGG + chr2_2:4816267-4816286 Msa0232530:intron 25.0%
!! TCAATTTTCAGATCTGTATA+GGG + chr2_2:4816268-4816287 Msa0232530:intron 25.0%
! ATCTATCACATAAAGCAAAT+TGG - chr2_2:4816298-4816317 None:intergenic 25.0%
! GTGATGTTATTGATGTATAA+AGG - chr2_2:4816666-4816685 None:intergenic 25.0%
! CTATTAACTCTGATATTTCT+AGG - chr2_2:4816805-4816824 None:intergenic 25.0%
! GAAATATCAGAGTTAATAGA+TGG + chr2_2:4816806-4816825 Msa0232530:intron 25.0%
!!! TTTGTGATTTGAAGTTTTAG+TGG - chr2_2:4816864-4816883 None:intergenic 25.0%
!! TAGCTAAACTCAAATTTGTT+GGG - chr2_2:4817001-4817020 None:intergenic 25.0%
! TAAACAAATTCAGTCTGAAT+TGG - chr2_2:4817093-4817112 None:intergenic 25.0%
!! TTTTGTGAAGATTAAAGTGA+AGG - chr2_2:4817179-4817198 None:intergenic 25.0%
!! GGTACATTTTGAATGTAATT+TGG - chr2_2:4817293-4817312 None:intergenic 25.0%
! CTTCAAATATATTTGCACAT+TGG - chr2_2:4817726-4817745 None:intergenic 25.0%
!! AATATATTTGAAGCACTTGT+TGG + chr2_2:4817733-4817752 Msa0232530:CDS 25.0%
!! ATATATTTGAAGCACTTGTT+GGG + chr2_2:4817734-4817753 Msa0232530:CDS 25.0%
!!! CGAAAAGTATTAACATATGA+AGG + chr2_2:4817778-4817797 Msa0232530:CDS 25.0%
!! CACACTTCTTTTATAGTTTT+GGG - chr2_2:4817890-4817909 None:intergenic 25.0%
!! ACACACTTCTTTTATAGTTT+TGG - chr2_2:4817891-4817910 None:intergenic 25.0%
! TATAAAACAAACACCCATAA+AGG - chr2_2:4817930-4817949 None:intergenic 25.0%
! CTATTCAACGTCATTTAATA+TGG + chr2_2:4817974-4817993 Msa0232530:three_prime_UTR 25.0%
!!! TTTTCTTGATGACAAGATTT+TGG - chr2_2:4818018-4818037 None:intergenic 25.0%
!!! CACAATTTTCTTTTTTTGAG+AGG - chr2_2:4818048-4818067 None:intergenic 25.0%
! GTTTCGAAATCTATAACTTT+AGG - chr2_2:4818072-4818091 None:intergenic 25.0%
AACAGTAACAAAAGTAACGA+TGG + chr2_2:4816559-4816578 Msa0232530:intron 30.0%
GATTTAATAGTCCACAAAAC+TGG - chr2_2:4816772-4816791 None:intergenic 30.0%
GTAGCTAAACTCAAATTTGT+TGG - chr2_2:4817002-4817021 None:intergenic 30.0%
AGTTTAGCTACTCATATACA+TGG + chr2_2:4817011-4817030 Msa0232530:intron 30.0%
GTGAAAATGTTATGTATTGG+TGG - chr2_2:4817314-4817333 None:intergenic 30.0%
TCATGTATCAGAAGAAACTA+TGG - chr2_2:4817378-4817397 None:intergenic 30.0%
AGAGAACATTGGAAGTAAAT+GGG - chr2_2:4817473-4817492 None:intergenic 30.0%
AAGATGAAAACAGAGAACAT+TGG - chr2_2:4817484-4817503 None:intergenic 30.0%
TGAGAAGAATGAACAAAATG+TGG + chr2_2:4817652-4817671 Msa0232530:intron 30.0%
GAGAAGAATGAACAAAATGT+GGG + chr2_2:4817653-4817672 Msa0232530:intron 30.0%
TGTATTGTGAAGGTTACATT+TGG + chr2_2:4817682-4817701 Msa0232530:intron 30.0%
! CTATAAAAGAAGTGTGTGAA+AGG + chr2_2:4817894-4817913 Msa0232530:three_prime_UTR 30.0%
TACAAATACAGCAAAACTGT+TGG - chr2_2:4818094-4818113 None:intergenic 30.0%
!! CAGTTTTGCTGTATTTGTAA+TGG + chr2_2:4818095-4818114 Msa0232530:three_prime_UTR 30.0%
AACAGTAACAAAAGTAACGA+TGG + chr2_2:4816559-4816578 Msa0232530:intron 30.0%
GATTTAATAGTCCACAAAAC+TGG - chr2_2:4816772-4816791 None:intergenic 30.0%
GTAGCTAAACTCAAATTTGT+TGG - chr2_2:4817002-4817021 None:intergenic 30.0%
AGTTTAGCTACTCATATACA+TGG + chr2_2:4817011-4817030 Msa0232530:intron 30.0%
GTGAAAATGTTATGTATTGG+TGG - chr2_2:4817314-4817333 None:intergenic 30.0%
TCATGTATCAGAAGAAACTA+TGG - chr2_2:4817378-4817397 None:intergenic 30.0%
AGAGAACATTGGAAGTAAAT+GGG - chr2_2:4817473-4817492 None:intergenic 30.0%
AAGATGAAAACAGAGAACAT+TGG - chr2_2:4817484-4817503 None:intergenic 30.0%
TGAGAAGAATGAACAAAATG+TGG + chr2_2:4817652-4817671 Msa0232530:intron 30.0%
GAGAAGAATGAACAAAATGT+GGG + chr2_2:4817653-4817672 Msa0232530:intron 30.0%
TGTATTGTGAAGGTTACATT+TGG + chr2_2:4817682-4817701 Msa0232530:intron 30.0%
! CTATAAAAGAAGTGTGTGAA+AGG + chr2_2:4817894-4817913 Msa0232530:three_prime_UTR 30.0%
TACAAATACAGCAAAACTGT+TGG - chr2_2:4818094-4818113 None:intergenic 30.0%
!! CAGTTTTGCTGTATTTGTAA+TGG + chr2_2:4818095-4818114 Msa0232530:three_prime_UTR 30.0%
TATCTGAGAAGACAACAAGT+TGG + chr2_2:4816114-4816133 Msa0232530:five_prime_UTR 35.0%
TCAATCAGATGGTTCTTACA+AGG + chr2_2:4816209-4816228 Msa0232530:CDS 35.0%
GATATACTTGTCTATGCTTG+TGG + chr2_2:4816315-4816334 Msa0232530:intron 35.0%
TCTGCATCTTGACTTCATAT+AGG + chr2_2:4816339-4816358 Msa0232530:intron 35.0%
CTTAATCTTGCATCAAACTG+TGG + chr2_2:4816367-4816386 Msa0232530:intron 35.0%
AACTTTGTTGAACAGCTTGA+AGG - chr2_2:4816499-4816518 None:intergenic 35.0%
ACATGTATCTTTGCTTGAGA+AGG - chr2_2:4816642-4816661 None:intergenic 35.0%
ATCATACCGTATAACAAGCA+TGG + chr2_2:4816883-4816902 Msa0232530:intron 35.0%
!! TGTTCTCCATGCTTGTTATA+CGG - chr2_2:4816892-4816911 None:intergenic 35.0%
! GACTGAATTTGTTTAGCACT+TGG + chr2_2:4817098-4817117 Msa0232530:intron 35.0%
AAGCGAATTTGTGTTTGCTT+TGG + chr2_2:4817130-4817149 Msa0232530:intron 35.0%
ATTAAAGTGAAGGAATGAGC+AGG - chr2_2:4817169-4817188 None:intergenic 35.0%
TGTATCAGAAGAAACTATGG+AGG - chr2_2:4817375-4817394 None:intergenic 35.0%
TAGACAGAAGAAACTATCTC+AGG - chr2_2:4817434-4817453 None:intergenic 35.0%
CAGAGAACATTGGAAGTAAA+TGG - chr2_2:4817474-4817493 None:intergenic 35.0%
AGAATGAACAAAATGTGGGT+TGG + chr2_2:4817657-4817676 Msa0232530:intron 35.0%
TATCTGAGAAGACAACAAGT+TGG + chr2_2:4816114-4816133 Msa0232530:five_prime_UTR 35.0%
TCAATCAGATGGTTCTTACA+AGG + chr2_2:4816209-4816228 Msa0232530:CDS 35.0%
GATATACTTGTCTATGCTTG+TGG + chr2_2:4816315-4816334 Msa0232530:intron 35.0%
TCTGCATCTTGACTTCATAT+AGG + chr2_2:4816339-4816358 Msa0232530:intron 35.0%
CTTAATCTTGCATCAAACTG+TGG + chr2_2:4816367-4816386 Msa0232530:intron 35.0%
AACTTTGTTGAACAGCTTGA+AGG - chr2_2:4816499-4816518 None:intergenic 35.0%
ACATGTATCTTTGCTTGAGA+AGG - chr2_2:4816642-4816661 None:intergenic 35.0%
ATCATACCGTATAACAAGCA+TGG + chr2_2:4816883-4816902 Msa0232530:intron 35.0%
!! TGTTCTCCATGCTTGTTATA+CGG - chr2_2:4816892-4816911 None:intergenic 35.0%
! GACTGAATTTGTTTAGCACT+TGG + chr2_2:4817098-4817117 Msa0232530:intron 35.0%
AAGCGAATTTGTGTTTGCTT+TGG + chr2_2:4817130-4817149 Msa0232530:intron 35.0%
ATTAAAGTGAAGGAATGAGC+AGG - chr2_2:4817169-4817188 None:intergenic 35.0%
TGTATCAGAAGAAACTATGG+AGG - chr2_2:4817375-4817394 None:intergenic 35.0%
TAGACAGAAGAAACTATCTC+AGG - chr2_2:4817434-4817453 None:intergenic 35.0%
CAGAGAACATTGGAAGTAAA+TGG - chr2_2:4817474-4817493 None:intergenic 35.0%
AGAATGAACAAAATGTGGGT+TGG + chr2_2:4817657-4817676 Msa0232530:intron 35.0%
GCCTAAAGGTAGAAATCAAG+AGG + chr2_2:4816175-4816194 Msa0232530:CDS 40.0%
GCCTCTTGATTTCTACCTTT+AGG - chr2_2:4816179-4816198 None:intergenic 40.0%
AAGGTAGAAATCAAGAGGCT+TGG + chr2_2:4816180-4816199 Msa0232530:CDS 40.0%
GAGAAAGAGAAGAGAAGAGA+AGG - chr2_2:4816247-4816266 None:intergenic 40.0%
! TAAACACATGGCCAGTTTTG+TGG + chr2_2:4816758-4816777 Msa0232530:intron 40.0%
AGTCTGAATTGGAGCTCATT+TGG - chr2_2:4817082-4817101 None:intergenic 40.0%
CTATGGAGGTAAAAAGTTGC+TGG - chr2_2:4817361-4817380 None:intergenic 40.0%
ATACATGACTGCTTGATCAC+AGG + chr2_2:4817390-4817409 Msa0232530:intron 40.0%
! TGGGTTGGATTGTATTGTGA+AGG + chr2_2:4817672-4817691 Msa0232530:intron 40.0%
GAGTGATTTCCACATTGTCA+TGG - chr2_2:4817825-4817844 None:intergenic 40.0%
TTGGGTAGATAGTGTTCAGT+TGG - chr2_2:4817872-4817891 None:intergenic 40.0%
GCCTAAAGGTAGAAATCAAG+AGG + chr2_2:4816175-4816194 Msa0232530:CDS 40.0%
GCCTCTTGATTTCTACCTTT+AGG - chr2_2:4816179-4816198 None:intergenic 40.0%
AAGGTAGAAATCAAGAGGCT+TGG + chr2_2:4816180-4816199 Msa0232530:CDS 40.0%
GAGAAAGAGAAGAGAAGAGA+AGG - chr2_2:4816247-4816266 None:intergenic 40.0%
! TAAACACATGGCCAGTTTTG+TGG + chr2_2:4816758-4816777 Msa0232530:intron 40.0%
AGTCTGAATTGGAGCTCATT+TGG - chr2_2:4817082-4817101 None:intergenic 40.0%
CTATGGAGGTAAAAAGTTGC+TGG - chr2_2:4817361-4817380 None:intergenic 40.0%
ATACATGACTGCTTGATCAC+AGG + chr2_2:4817390-4817409 Msa0232530:intron 40.0%
! TGGGTTGGATTGTATTGTGA+AGG + chr2_2:4817672-4817691 Msa0232530:intron 40.0%
GAGTGATTTCCACATTGTCA+TGG - chr2_2:4817825-4817844 None:intergenic 40.0%
TTGGGTAGATAGTGTTCAGT+TGG - chr2_2:4817872-4817891 None:intergenic 40.0%
! AAGCAGAAGAAGCATGAACG+TGG + chr2_2:4816137-4816156 Msa0232530:exon 45.0%
AAGCATGAACGTGGAAGAAG+AGG + chr2_2:4816146-4816165 Msa0232530:exon 45.0%
ATGAACGTGGAAGAAGAGGT+TGG + chr2_2:4816150-4816169 Msa0232530:CDS 45.0%
CTTGGCAAGCTTCAATCAGA+TGG + chr2_2:4816198-4816217 Msa0232530:CDS 45.0%
! AAGCAGAAGAAGCATGAACG+TGG + chr2_2:4816137-4816156 Msa0232530:exon 45.0%
AAGCATGAACGTGGAAGAAG+AGG + chr2_2:4816146-4816165 Msa0232530:exon 45.0%
ATGAACGTGGAAGAAGAGGT+TGG + chr2_2:4816150-4816169 Msa0232530:CDS 45.0%
CTTGGCAAGCTTCAATCAGA+TGG + chr2_2:4816198-4816217 Msa0232530:CDS 45.0%
!! ATAAAATAATAAAAAAAGAT+TGG + chr2_2:4818144-4818163 Msa0232530:three_prime_UTR 5.0%
!! TAAAATAATAAAAAAAGATT+GGG + chr2_2:4818145-4818164 Msa0232530:three_prime_UTR 5.0%
!! ATAAAATAATAAAAAAAGAT+TGG + chr2_2:4818144-4818163 Msa0232530:three_prime_UTR 5.0%
!! TAAAATAATAAAAAAAGATT+GGG + chr2_2:4818145-4818164 Msa0232530:three_prime_UTR 5.0%
AGAAGAGGTTGGACGCCTAA+AGG + chr2_2:4816161-4816180 Msa0232530:CDS 50.0%
GAAGGTGAATTGCTGCTGCA+AGG + chr2_2:4817796-4817815 Msa0232530:CDS 50.0%
GCAAGGAGTCCATGACAATG+TGG + chr2_2:4817813-4817832 Msa0232530:CDS 50.0%
TTCAGTTGGAAGCAGCAGCA+GGG - chr2_2:4817858-4817877 None:intergenic 50.0%
AGAAGAGGTTGGACGCCTAA+AGG + chr2_2:4816161-4816180 Msa0232530:CDS 50.0%
GAAGGTGAATTGCTGCTGCA+AGG + chr2_2:4817796-4817815 Msa0232530:CDS 50.0%
GCAAGGAGTCCATGACAATG+TGG + chr2_2:4817813-4817832 Msa0232530:CDS 50.0%
TTCAGTTGGAAGCAGCAGCA+GGG - chr2_2:4817858-4817877 None:intergenic 50.0%
TCAGTTGGAAGCAGCAGCAG+GGG - chr2_2:4817857-4817876 None:intergenic 55.0%
GTTCAGTTGGAAGCAGCAGC+AGG - chr2_2:4817859-4817878 None:intergenic 55.0%
TCAGTTGGAAGCAGCAGCAG+GGG - chr2_2:4817857-4817876 None:intergenic 55.0%
GTTCAGTTGGAAGCAGCAGC+AGG - chr2_2:4817859-4817878 None:intergenic 55.0%
TTGGAAGCAGCAGCAGGGGT+GGG - chr2_2:4817853-4817872 None:intergenic 60.0%
TTGGAAGCAGCAGCAGGGGT+GGG - chr2_2:4817853-4817872 None:intergenic 60.0%
GTTGGAAGCAGCAGCAGGGG+TGG - chr2_2:4817854-4817873 None:intergenic 65.0%
GTTGGAAGCAGCAGCAGGGG+TGG - chr2_2:4817854-4817873 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 4816061 4818175 4816061 ID=Msa0232530;Name=Msa0232530
chr2_2 mRNA 4816061 4818175 4816061 ID=Msa0232530-mRNA-1;Parent=Msa0232530;Name=Msa0232530-mRNA-1;_AED=0.03;_eAED=0.03;_QI=89|1|1|1|1|1|2|299|87
chr2_2 exon 4816061 4816230 4816061 ID=Msa0232530-mRNA-1:exon:2041;Parent=Msa0232530-mRNA-1
chr2_2 exon 4817694 4818175 4817694 ID=Msa0232530-mRNA-1:exon:2042;Parent=Msa0232530-mRNA-1
chr2_2 five_prime_UTR 4816061 4816149 4816061 ID=Msa0232530-mRNA-1:five_prime_utr;Parent=Msa0232530-mRNA-1
chr2_2 CDS 4816150 4816230 4816150 ID=Msa0232530-mRNA-1:cds;Parent=Msa0232530-mRNA-1
chr2_2 CDS 4817694 4817876 4817694 ID=Msa0232530-mRNA-1:cds;Parent=Msa0232530-mRNA-1
chr2_2 three_prime_UTR 4817877 4818175 4817877 ID=Msa0232530-mRNA-1:three_prime_utr;Parent=Msa0232530-mRNA-1
Gene Sequence

>Msa0232530

ATGAACGTGGAAGAAGAGGTTGGACGCCTAAAGGTAGAAATCAAGAGGCTTGGCAAGCTTCAATCAGATGGTTCTTACAAGGTTACATTTGGAACGCTATTTCATGATGACCAATGTGCAAATATATTTGAAGCACTTGTTGGGACACTAAGAGCAGCTAAAAAACGAAAAGTATTAACATATGAAGGTGAATTGCTGCTGCAAGGAGTCCATGACAATGTGGAAATCACTCTTAATCCCACCCCTGCTGCTGCTTCCAACTGA
Protein sequence

>Msa0232530

MNVEEEVGRLKVEIKRLGKLQSDGSYKVTFGTLFHDDQCANIFEALVGTLRAAKKRKVLTYEGELLLQGVHDNVEITLNPTPAAASN*