AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0271910

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0271910 MtrunA17_Chr2g0279651 96.552 87 3 0 1 87 1 87 1.39e-58 174
Msa0271910 MtrunA17_Chr4g0049791 84.615 78 12 0 1 78 1 78 1.82e-38 123
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0271910 AT4G33640.1 76.923 78 18 0 1 78 1 78 4.22e-40 127
Msa0271910 AT4G33640.2 76.923 78 18 0 1 78 67 144 1.34e-39 129

Find 33 sgRNAs with CRISPR-Local

Find 213 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTATTCAACGTCATTTAATA+TGG 0.239487 2_3:-3421677 Msa0271910:three_prime_UTR
GTTTCGAAATCTATAACTTT+AGG 0.262910 2_3:+3421582 None:intergenic
TATAAAACAAACACCCATAA+AGG 0.380566 2_3:+3421724 None:intergenic
TGTATTGTGAAGGTTACATT+TGG 0.382119 2_3:-3421969 Msa0271910:intron
ATATCTTCCACCTGATTGAT+AGG 0.386810 2_3:+3423797 None:intergenic
GCCTCTTGATTTCTACCTTT+AGG 0.396207 2_3:+3423686 None:intergenic
AATATATTTGAAGCACTTGT+TGG 0.411547 2_3:-3421918 Msa0271910:CDS
CCTTGTAAGAACCATCTGAT+TGG 0.444815 2_3:+3423653 None:intergenic
GTTCAGTTGGAAGCAGCAGC+AGG 0.447332 2_3:+3421795 None:intergenic
TAAATATCAGCCTATCAATC+AGG 0.457446 2_3:-3423807 Msa0271910:five_prime_UTR
ATATATTTGAAGCACTTGTT+GGG 0.466568 2_3:-3421917 Msa0271910:CDS
TTGGGTAGATAGTGTTCAGT+TGG 0.483464 2_3:+3421782 None:intergenic
CGAAAAGTATTAACATATGA+AGG 0.490626 2_3:-3421873 Msa0271910:CDS
TACAAATACAGCAAAACTGT+TGG 0.506470 2_3:+3421560 None:intergenic
CCAATCAGATGGTTCTTACA+AGG 0.510812 2_3:-3423653 Msa0271910:intron
AGAAGAGGTTGGACGCCTAA+AGG 0.519063 2_3:-3423701 Msa0271910:CDS
CTATAAAAGAAGTGTGTGAA+AGG 0.528769 2_3:-3421757 Msa0271910:three_prime_UTR
GAGTGATTTCCACATTGTCA+TGG 0.529569 2_3:+3421829 None:intergenic
CTTCAAATATATTTGCACAT+TGG 0.530146 2_3:+3421928 None:intergenic
AAGGTAGAAATCAAGAGGCT+TGG 0.545059 2_3:-3423682 Msa0271910:CDS
TTCAGTTGGAAGCAGCAGCA+GGG 0.553467 2_3:+3421796 None:intergenic
GTTGGAAGCAGCAGCAGGGG+TGG 0.553565 2_3:+3421800 None:intergenic
TTGGAAGCAGCAGCAGGGGT+GGG 0.565500 2_3:+3421801 None:intergenic
AAGCATGAACGTGGAAGAAG+AGG 0.576730 2_3:-3423716 Msa0271910:exon
CTTGGCAAGCTCCAATCAGA+TGG 0.580481 2_3:-3423664 Msa0271910:CDS
TATCTGAGAAGACAACAAGT+TGG 0.597136 2_3:-3423748 Msa0271910:five_prime_UTR
ATATCAGCCTATCAATCAGG+TGG 0.617592 2_3:-3423804 Msa0271910:five_prime_UTR
GCCTAAAGGTAGAAATCAAG+AGG 0.618558 2_3:-3423687 Msa0271910:CDS
ATGAACGTGGAAGAAGAGGT+TGG 0.620804 2_3:-3423712 Msa0271910:CDS
TCAGTTGGAAGCAGCAGCAG+GGG 0.624373 2_3:+3421797 None:intergenic
GAAGGTGAATTGCTGCTGCA+AGG 0.659800 2_3:-3421855 Msa0271910:CDS
AAGCAGAAGAAGCATGAACG+TGG 0.660179 2_3:-3423725 Msa0271910:exon
GCAAGGAGTCCATGACAATG+TGG 0.727047 2_3:-3421838 Msa0271910:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAATAAAATAAATAATA+AGG + chr2_3:3423267-3423286 None:intergenic 0.0%
!! AAAAAATAAAATAAATAATA+AGG + chr2_3:3423267-3423286 None:intergenic 0.0%
!!! TTATATATAGTTATTTTGAA+AGG + chr2_3:3421941-3421960 None:intergenic 10.0%
!!! TTATATATAGTTATTTTGAA+AGG + chr2_3:3421941-3421960 None:intergenic 10.0%
!!! AAATTTTAGAATTTATCACT+TGG - chr2_3:3422040-3422059 Msa0271910:intron 15.0%
!! AAGTTATAAATAGTTTCAAT+TGG + chr2_3:3422756-3422775 None:intergenic 15.0%
!! ATTGAAACTATTTATAACTT+TGG - chr2_3:3422756-3422775 Msa0271910:intron 15.0%
!! TTGAAACTATTTATAACTTT+GGG - chr2_3:3422757-3422776 Msa0271910:intron 15.0%
!!! ATAACATTTTCACTTAAAAT+TGG - chr2_3:3422831-3422850 Msa0271910:intron 15.0%
!! CTAATAAACATAATAGAATA+TGG - chr2_3:3423022-3423041 Msa0271910:intron 15.0%
!!! AAATTTTAGAATTTATCACT+TGG - chr2_3:3422040-3422059 Msa0271910:intron 15.0%
!! AAGTTATAAATAGTTTCAAT+TGG + chr2_3:3422756-3422775 None:intergenic 15.0%
!! ATTGAAACTATTTATAACTT+TGG - chr2_3:3422756-3422775 Msa0271910:intron 15.0%
!! TTGAAACTATTTATAACTTT+GGG - chr2_3:3422757-3422776 Msa0271910:intron 15.0%
!!! ATAACATTTTCACTTAAAAT+TGG - chr2_3:3422831-3422850 Msa0271910:intron 15.0%
!! CTAATAAACATAATAGAATA+TGG - chr2_3:3423022-3423041 Msa0271910:intron 15.0%
!!! GTGGAAGATATTTTAAATTA+AGG - chr2_3:3421583-3421602 Msa0271910:three_prime_UTR 20.0%
!! TGTATTATATTCTTAGTTCA+TGG - chr2_3:3421915-3421934 Msa0271910:CDS 20.0%
!! GATAATATGTGATTATAAGA+TGG + chr2_3:3422198-3422217 None:intergenic 20.0%
!! TTTAAATGTCTTTGTTAAGA+CGG + chr2_3:3422247-3422266 None:intergenic 20.0%
!! AGACATTTAAATTAAACACA+TGG - chr2_3:3422256-3422275 Msa0271910:intron 20.0%
!!! TAGTTTTTGAATCTTGATTT+AGG + chr2_3:3422347-3422366 None:intergenic 20.0%
!! TTATAAATAGTTTCAATTGG+TGG + chr2_3:3422753-3422772 None:intergenic 20.0%
!! TGAAACTATTTATAACTTTG+GGG - chr2_3:3422758-3422777 Msa0271910:intron 20.0%
!! TAAGTGAAAATGTTATGTAT+TGG + chr2_3:3422827-3422846 None:intergenic 20.0%
!! TCTGTCTATATACATAAATT+AGG - chr2_3:3422956-3422975 Msa0271910:intron 20.0%
!! CTGTCTATATACATAAATTA+GGG - chr2_3:3422957-3422976 Msa0271910:intron 20.0%
!! AAGGATAAAAATAACCTTTA+TGG - chr2_3:3423630-3423649 Msa0271910:intron 20.0%
!! AGGATAAAAATAACCTTTAT+GGG - chr2_3:3423631-3423650 Msa0271910:intron 20.0%
!!! GTGGAAGATATTTTAAATTA+AGG - chr2_3:3421583-3421602 Msa0271910:three_prime_UTR 20.0%
!! TGTATTATATTCTTAGTTCA+TGG - chr2_3:3421915-3421934 Msa0271910:CDS 20.0%
!! GATAATATGTGATTATAAGA+TGG + chr2_3:3422198-3422217 None:intergenic 20.0%
!! TTTAAATGTCTTTGTTAAGA+CGG + chr2_3:3422247-3422266 None:intergenic 20.0%
!! AGACATTTAAATTAAACACA+TGG - chr2_3:3422256-3422275 Msa0271910:intron 20.0%
!!! TAGTTTTTGAATCTTGATTT+AGG + chr2_3:3422347-3422366 None:intergenic 20.0%
!! TTATAAATAGTTTCAATTGG+TGG + chr2_3:3422753-3422772 None:intergenic 20.0%
!! TGAAACTATTTATAACTTTG+GGG - chr2_3:3422758-3422777 Msa0271910:intron 20.0%
!! TAAGTGAAAATGTTATGTAT+TGG + chr2_3:3422827-3422846 None:intergenic 20.0%
!! TCTGTCTATATACATAAATT+AGG - chr2_3:3422956-3422975 Msa0271910:intron 20.0%
!! CTGTCTATATACATAAATTA+GGG - chr2_3:3422957-3422976 Msa0271910:intron 20.0%
!! AAGGATAAAAATAACCTTTA+TGG - chr2_3:3423630-3423649 Msa0271910:intron 20.0%
!! AGGATAAAAATAACCTTTAT+GGG - chr2_3:3423631-3423650 Msa0271910:intron 20.0%
!! TTCAATTTTCAGATCTGTAT+AGG - chr2_3:3421782-3421801 Msa0271910:exon 25.0%
!! TCAATTTTCAGATCTGTATA+GGG - chr2_3:3421783-3421802 Msa0271910:exon 25.0%
! ATCTATCACATAAAGCAAAT+TGG + chr2_3:3421813-3421832 None:intergenic 25.0%
! GTGATGTTATTGATGTATAA+AGG + chr2_3:3422176-3422195 None:intergenic 25.0%
! CTATTAACTCTGATATTTCT+AGG + chr2_3:3422315-3422334 None:intergenic 25.0%
! GAAATATCAGAGTTAATAGA+TGG - chr2_3:3422316-3422335 Msa0271910:intron 25.0%
!!! TTTGTGATTTGAAGTTTTAG+TGG + chr2_3:3422374-3422393 None:intergenic 25.0%
!! TAGCTAAACTCAAATTTGTT+GGG + chr2_3:3422511-3422530 None:intergenic 25.0%
! TAAACAAATTCAGTCTGAAT+TGG + chr2_3:3422603-3422622 None:intergenic 25.0%
!! TTTTGTGAAGATTAAAGTGA+AGG + chr2_3:3422689-3422708 None:intergenic 25.0%
!! GGTACATTTTGAATGTAATT+TGG + chr2_3:3422803-3422822 None:intergenic 25.0%
!!! ACAGTTGTCAGAATTTTAAT+TGG - chr2_3:3423087-3423106 Msa0271910:intron 25.0%
! CTTCAAATATATTTGCACAT+TGG + chr2_3:3423443-3423462 None:intergenic 25.0%
!! AATATATTTGAAGCACTTGT+TGG - chr2_3:3423450-3423469 Msa0271910:intron 25.0%
!! ATATATTTGAAGCACTTGTT+GGG - chr2_3:3423451-3423470 Msa0271910:intron 25.0%
!!! CGAAAAGTATTAACATATGA+AGG - chr2_3:3423495-3423514 Msa0271910:intron 25.0%
!! CACACTTCTTTTATAGTTTT+GGG + chr2_3:3423607-3423626 None:intergenic 25.0%
!! ACACACTTCTTTTATAGTTT+TGG + chr2_3:3423608-3423627 None:intergenic 25.0%
! TATAAAACAAACACCCATAA+AGG + chr2_3:3423647-3423666 None:intergenic 25.0%
! CTATTCAACGTCATTTAATA+TGG - chr2_3:3423691-3423710 Msa0271910:CDS 25.0%
!!! TTTTCTTGATGACAAGATTT+TGG + chr2_3:3423735-3423754 None:intergenic 25.0%
!!! CACAATTTTCTTTTTTTGAG+AGG + chr2_3:3423765-3423784 None:intergenic 25.0%
! GTTTCGAAATCTATAACTTT+AGG + chr2_3:3423789-3423808 None:intergenic 25.0%
! GTGTTACATCGTTAAAAATT+TGG + chr2_3:3421500-3421519 None:intergenic 25.0%
!! TTCAATTTTCAGATCTGTAT+AGG - chr2_3:3421782-3421801 Msa0271910:exon 25.0%
!! TCAATTTTCAGATCTGTATA+GGG - chr2_3:3421783-3421802 Msa0271910:exon 25.0%
! ATCTATCACATAAAGCAAAT+TGG + chr2_3:3421813-3421832 None:intergenic 25.0%
! GTGATGTTATTGATGTATAA+AGG + chr2_3:3422176-3422195 None:intergenic 25.0%
! CTATTAACTCTGATATTTCT+AGG + chr2_3:3422315-3422334 None:intergenic 25.0%
! GAAATATCAGAGTTAATAGA+TGG - chr2_3:3422316-3422335 Msa0271910:intron 25.0%
!!! TTTGTGATTTGAAGTTTTAG+TGG + chr2_3:3422374-3422393 None:intergenic 25.0%
!! TAGCTAAACTCAAATTTGTT+GGG + chr2_3:3422511-3422530 None:intergenic 25.0%
! TAAACAAATTCAGTCTGAAT+TGG + chr2_3:3422603-3422622 None:intergenic 25.0%
!! TTTTGTGAAGATTAAAGTGA+AGG + chr2_3:3422689-3422708 None:intergenic 25.0%
!! GGTACATTTTGAATGTAATT+TGG + chr2_3:3422803-3422822 None:intergenic 25.0%
!!! ACAGTTGTCAGAATTTTAAT+TGG - chr2_3:3423087-3423106 Msa0271910:intron 25.0%
! CTTCAAATATATTTGCACAT+TGG + chr2_3:3423443-3423462 None:intergenic 25.0%
!! AATATATTTGAAGCACTTGT+TGG - chr2_3:3423450-3423469 Msa0271910:intron 25.0%
!! ATATATTTGAAGCACTTGTT+GGG - chr2_3:3423451-3423470 Msa0271910:intron 25.0%
!!! CGAAAAGTATTAACATATGA+AGG - chr2_3:3423495-3423514 Msa0271910:intron 25.0%
!! CACACTTCTTTTATAGTTTT+GGG + chr2_3:3423607-3423626 None:intergenic 25.0%
!! ACACACTTCTTTTATAGTTT+TGG + chr2_3:3423608-3423627 None:intergenic 25.0%
! TATAAAACAAACACCCATAA+AGG + chr2_3:3423647-3423666 None:intergenic 25.0%
! CTATTCAACGTCATTTAATA+TGG - chr2_3:3423691-3423710 Msa0271910:CDS 25.0%
!!! TTTTCTTGATGACAAGATTT+TGG + chr2_3:3423735-3423754 None:intergenic 25.0%
!!! CACAATTTTCTTTTTTTGAG+AGG + chr2_3:3423765-3423784 None:intergenic 25.0%
! GTTTCGAAATCTATAACTTT+AGG + chr2_3:3423789-3423808 None:intergenic 25.0%
TAAATATCAGCCTATCAATC+AGG - chr2_3:3421561-3421580 Msa0271910:three_prime_UTR 30.0%
AACAGTAACAAAAGTAACGA+TGG - chr2_3:3422069-3422088 Msa0271910:intron 30.0%
GATTTAATAGTCCACAAAAC+TGG + chr2_3:3422282-3422301 None:intergenic 30.0%
GTAGCTAAACTCAAATTTGT+TGG + chr2_3:3422512-3422531 None:intergenic 30.0%
AGTTTAGCTACTCATATACA+TGG - chr2_3:3422521-3422540 Msa0271910:intron 30.0%
GTGAAAATGTTATGTATTGG+TGG + chr2_3:3422824-3422843 None:intergenic 30.0%
TCATGTATCAGAAGAAACTA+TGG + chr2_3:3422888-3422907 None:intergenic 30.0%
AGAGAACATTGGAAGTAAAT+GGG + chr2_3:3422983-3423002 None:intergenic 30.0%
AAGATGAAAACAGAGAACAT+TGG + chr2_3:3422994-3423013 None:intergenic 30.0%
TGAGAAGAATGAACAAAATG+TGG - chr2_3:3423369-3423388 Msa0271910:intron 30.0%
GAGAAGAATGAACAAAATGT+GGG - chr2_3:3423370-3423389 Msa0271910:intron 30.0%
TGTATTGTGAAGGTTACATT+TGG - chr2_3:3423399-3423418 Msa0271910:intron 30.0%
! CTATAAAAGAAGTGTGTGAA+AGG - chr2_3:3423611-3423630 Msa0271910:intron 30.0%
TACAAATACAGCAAAACTGT+TGG + chr2_3:3423811-3423830 None:intergenic 30.0%
!! CAGTTTTGCTGTATTTGTAA+TGG - chr2_3:3423812-3423831 Msa0271910:five_prime_UTR 30.0%
TAAATATCAGCCTATCAATC+AGG - chr2_3:3421561-3421580 Msa0271910:three_prime_UTR 30.0%
AACAGTAACAAAAGTAACGA+TGG - chr2_3:3422069-3422088 Msa0271910:intron 30.0%
GATTTAATAGTCCACAAAAC+TGG + chr2_3:3422282-3422301 None:intergenic 30.0%
GTAGCTAAACTCAAATTTGT+TGG + chr2_3:3422512-3422531 None:intergenic 30.0%
AGTTTAGCTACTCATATACA+TGG - chr2_3:3422521-3422540 Msa0271910:intron 30.0%
GTGAAAATGTTATGTATTGG+TGG + chr2_3:3422824-3422843 None:intergenic 30.0%
TCATGTATCAGAAGAAACTA+TGG + chr2_3:3422888-3422907 None:intergenic 30.0%
AGAGAACATTGGAAGTAAAT+GGG + chr2_3:3422983-3423002 None:intergenic 30.0%
AAGATGAAAACAGAGAACAT+TGG + chr2_3:3422994-3423013 None:intergenic 30.0%
TGAGAAGAATGAACAAAATG+TGG - chr2_3:3423369-3423388 Msa0271910:intron 30.0%
GAGAAGAATGAACAAAATGT+GGG - chr2_3:3423370-3423389 Msa0271910:intron 30.0%
TGTATTGTGAAGGTTACATT+TGG - chr2_3:3423399-3423418 Msa0271910:intron 30.0%
! CTATAAAAGAAGTGTGTGAA+AGG - chr2_3:3423611-3423630 Msa0271910:intron 30.0%
TACAAATACAGCAAAACTGT+TGG + chr2_3:3423811-3423830 None:intergenic 30.0%
!! CAGTTTTGCTGTATTTGTAA+TGG - chr2_3:3423812-3423831 Msa0271910:five_prime_UTR 30.0%
ATATCTTCCACCTGATTGAT+AGG + chr2_3:3421574-3421593 None:intergenic 35.0%
TATCTGAGAAGACAACAAGT+TGG - chr2_3:3421620-3421639 Msa0271910:three_prime_UTR 35.0%
GATATACTTGTCTATGCTTG+TGG - chr2_3:3421830-3421849 Msa0271910:CDS 35.0%
TCTGCATCTTGACTTCATAT+AGG - chr2_3:3421854-3421873 Msa0271910:CDS 35.0%
CTTAATCTTGCATCAAACTG+TGG - chr2_3:3421882-3421901 Msa0271910:CDS 35.0%
AACTTTGTTGAACAGCTTGA+AGG + chr2_3:3422009-3422028 None:intergenic 35.0%
ACATGTATCTTTGCTTGAGA+AGG + chr2_3:3422152-3422171 None:intergenic 35.0%
ATCATACCGTATAACAAGCA+TGG - chr2_3:3422393-3422412 Msa0271910:intron 35.0%
!! TGTTCTCCATGCTTGTTATA+CGG + chr2_3:3422402-3422421 None:intergenic 35.0%
! GACTGAATTTGTTTAGCACT+TGG - chr2_3:3422608-3422627 Msa0271910:intron 35.0%
AAGCGAATTTGTGTTTGCTT+TGG - chr2_3:3422640-3422659 Msa0271910:intron 35.0%
ATTAAAGTGAAGGAATGAGC+AGG + chr2_3:3422679-3422698 None:intergenic 35.0%
TGTATCAGAAGAAACTATGG+AGG + chr2_3:3422885-3422904 None:intergenic 35.0%
TAGACAGAAGAAACTATCTC+AGG + chr2_3:3422944-3422963 None:intergenic 35.0%
CAGAGAACATTGGAAGTAAA+TGG + chr2_3:3422984-3423003 None:intergenic 35.0%
! ATGGAAAATGCTACTTCTCA+AGG - chr2_3:3423041-3423060 Msa0271910:intron 35.0%
! TGCACTACATTTTCATTCAG+TGG + chr2_3:3423144-3423163 None:intergenic 35.0%
TGCAAATAGTGCATTGTGAT+TGG - chr2_3:3423160-3423179 Msa0271910:intron 35.0%
AGAATGAACAAAATGTGGGT+TGG - chr2_3:3423374-3423393 Msa0271910:intron 35.0%
ATATCTTCCACCTGATTGAT+AGG + chr2_3:3421574-3421593 None:intergenic 35.0%
TATCTGAGAAGACAACAAGT+TGG - chr2_3:3421620-3421639 Msa0271910:three_prime_UTR 35.0%
GATATACTTGTCTATGCTTG+TGG - chr2_3:3421830-3421849 Msa0271910:CDS 35.0%
TCTGCATCTTGACTTCATAT+AGG - chr2_3:3421854-3421873 Msa0271910:CDS 35.0%
CTTAATCTTGCATCAAACTG+TGG - chr2_3:3421882-3421901 Msa0271910:CDS 35.0%
AACTTTGTTGAACAGCTTGA+AGG + chr2_3:3422009-3422028 None:intergenic 35.0%
ACATGTATCTTTGCTTGAGA+AGG + chr2_3:3422152-3422171 None:intergenic 35.0%
ATCATACCGTATAACAAGCA+TGG - chr2_3:3422393-3422412 Msa0271910:intron 35.0%
!! TGTTCTCCATGCTTGTTATA+CGG + chr2_3:3422402-3422421 None:intergenic 35.0%
! GACTGAATTTGTTTAGCACT+TGG - chr2_3:3422608-3422627 Msa0271910:intron 35.0%
AAGCGAATTTGTGTTTGCTT+TGG - chr2_3:3422640-3422659 Msa0271910:intron 35.0%
ATTAAAGTGAAGGAATGAGC+AGG + chr2_3:3422679-3422698 None:intergenic 35.0%
TGTATCAGAAGAAACTATGG+AGG + chr2_3:3422885-3422904 None:intergenic 35.0%
TAGACAGAAGAAACTATCTC+AGG + chr2_3:3422944-3422963 None:intergenic 35.0%
CAGAGAACATTGGAAGTAAA+TGG + chr2_3:3422984-3423003 None:intergenic 35.0%
! ATGGAAAATGCTACTTCTCA+AGG - chr2_3:3423041-3423060 Msa0271910:intron 35.0%
! TGCACTACATTTTCATTCAG+TGG + chr2_3:3423144-3423163 None:intergenic 35.0%
TGCAAATAGTGCATTGTGAT+TGG - chr2_3:3423160-3423179 Msa0271910:intron 35.0%
AGAATGAACAAAATGTGGGT+TGG - chr2_3:3423374-3423393 Msa0271910:intron 35.0%
ATATCAGCCTATCAATCAGG+TGG - chr2_3:3421564-3421583 Msa0271910:three_prime_UTR 40.0%
GCCTAAAGGTAGAAATCAAG+AGG - chr2_3:3421681-3421700 Msa0271910:three_prime_UTR 40.0%
GCCTCTTGATTTCTACCTTT+AGG + chr2_3:3421685-3421704 None:intergenic 40.0%
AAGGTAGAAATCAAGAGGCT+TGG - chr2_3:3421686-3421705 Msa0271910:three_prime_UTR 40.0%
CCTTGTAAGAACCATCTGAT+TGG + chr2_3:3421718-3421737 None:intergenic 40.0%
CCAATCAGATGGTTCTTACA+AGG - chr2_3:3421715-3421734 Msa0271910:three_prime_UTR 40.0%
AGAGAAGAGAAGAGAAGAGA+AGG + chr2_3:3421753-3421772 None:intergenic 40.0%
! TAAACACATGGCCAGTTTTG+TGG - chr2_3:3422268-3422287 Msa0271910:intron 40.0%
AGTCTGAATTGGAGCTCATT+TGG + chr2_3:3422592-3422611 None:intergenic 40.0%
CTATGGAGGTAAAAAGTTGC+TGG + chr2_3:3422871-3422890 None:intergenic 40.0%
ATACATGACTGCTTGATCAC+AGG - chr2_3:3422900-3422919 Msa0271910:intron 40.0%
! TGGGTTGGATTGTATTGTGA+AGG - chr2_3:3423389-3423408 Msa0271910:intron 40.0%
GAGTGATTTCCACATTGTCA+TGG + chr2_3:3423542-3423561 None:intergenic 40.0%
TTGGGTAGATAGTGTTCAGT+TGG + chr2_3:3423589-3423608 None:intergenic 40.0%
ATATCAGCCTATCAATCAGG+TGG - chr2_3:3421564-3421583 Msa0271910:three_prime_UTR 40.0%
GCCTAAAGGTAGAAATCAAG+AGG - chr2_3:3421681-3421700 Msa0271910:three_prime_UTR 40.0%
GCCTCTTGATTTCTACCTTT+AGG + chr2_3:3421685-3421704 None:intergenic 40.0%
AAGGTAGAAATCAAGAGGCT+TGG - chr2_3:3421686-3421705 Msa0271910:three_prime_UTR 40.0%
CCTTGTAAGAACCATCTGAT+TGG + chr2_3:3421718-3421737 None:intergenic 40.0%
CCAATCAGATGGTTCTTACA+AGG - chr2_3:3421715-3421734 Msa0271910:three_prime_UTR 40.0%
AGAGAAGAGAAGAGAAGAGA+AGG + chr2_3:3421753-3421772 None:intergenic 40.0%
! TAAACACATGGCCAGTTTTG+TGG - chr2_3:3422268-3422287 Msa0271910:intron 40.0%
AGTCTGAATTGGAGCTCATT+TGG + chr2_3:3422592-3422611 None:intergenic 40.0%
CTATGGAGGTAAAAAGTTGC+TGG + chr2_3:3422871-3422890 None:intergenic 40.0%
ATACATGACTGCTTGATCAC+AGG - chr2_3:3422900-3422919 Msa0271910:intron 40.0%
! TGGGTTGGATTGTATTGTGA+AGG - chr2_3:3423389-3423408 Msa0271910:intron 40.0%
GAGTGATTTCCACATTGTCA+TGG + chr2_3:3423542-3423561 None:intergenic 40.0%
TTGGGTAGATAGTGTTCAGT+TGG + chr2_3:3423589-3423608 None:intergenic 40.0%
! AAGCAGAAGAAGCATGAACG+TGG - chr2_3:3421643-3421662 Msa0271910:three_prime_UTR 45.0%
AAGCATGAACGTGGAAGAAG+AGG - chr2_3:3421652-3421671 Msa0271910:three_prime_UTR 45.0%
ATGAACGTGGAAGAAGAGGT+TGG - chr2_3:3421656-3421675 Msa0271910:three_prime_UTR 45.0%
! AAGCAGAAGAAGCATGAACG+TGG - chr2_3:3421643-3421662 Msa0271910:three_prime_UTR 45.0%
AAGCATGAACGTGGAAGAAG+AGG - chr2_3:3421652-3421671 Msa0271910:three_prime_UTR 45.0%
ATGAACGTGGAAGAAGAGGT+TGG - chr2_3:3421656-3421675 Msa0271910:three_prime_UTR 45.0%
!! ATAAAATAATAAAAAAAGAT+TGG - chr2_3:3423861-3423880 Msa0271910:five_prime_UTR 5.0%
!! TAAAATAATAAAAAAAGATT+GGG - chr2_3:3423862-3423881 Msa0271910:five_prime_UTR 5.0%
!! ATAAAATAATAAAAAAAGAT+TGG - chr2_3:3423861-3423880 Msa0271910:five_prime_UTR 5.0%
!! TAAAATAATAAAAAAAGATT+GGG - chr2_3:3423862-3423881 Msa0271910:five_prime_UTR 5.0%
AGAAGAGGTTGGACGCCTAA+AGG - chr2_3:3421667-3421686 Msa0271910:three_prime_UTR 50.0%
CTTGGCAAGCTCCAATCAGA+TGG - chr2_3:3421704-3421723 Msa0271910:three_prime_UTR 50.0%
GAAGGTGAATTGCTGCTGCA+AGG - chr2_3:3423513-3423532 Msa0271910:intron 50.0%
GCAAGGAGTCCATGACAATG+TGG - chr2_3:3423530-3423549 Msa0271910:intron 50.0%
TTCAGTTGGAAGCAGCAGCA+GGG + chr2_3:3423575-3423594 None:intergenic 50.0%
AGAAGAGGTTGGACGCCTAA+AGG - chr2_3:3421667-3421686 Msa0271910:three_prime_UTR 50.0%
CTTGGCAAGCTCCAATCAGA+TGG - chr2_3:3421704-3421723 Msa0271910:three_prime_UTR 50.0%
GAAGGTGAATTGCTGCTGCA+AGG - chr2_3:3423513-3423532 Msa0271910:intron 50.0%
GCAAGGAGTCCATGACAATG+TGG - chr2_3:3423530-3423549 Msa0271910:intron 50.0%
TTCAGTTGGAAGCAGCAGCA+GGG + chr2_3:3423575-3423594 None:intergenic 50.0%
TCAGTTGGAAGCAGCAGCAG+GGG + chr2_3:3423574-3423593 None:intergenic 55.0%
GTTCAGTTGGAAGCAGCAGC+AGG + chr2_3:3423576-3423595 None:intergenic 55.0%
TCAGTTGGAAGCAGCAGCAG+GGG + chr2_3:3423574-3423593 None:intergenic 55.0%
GTTCAGTTGGAAGCAGCAGC+AGG + chr2_3:3423576-3423595 None:intergenic 55.0%
TTGGAAGCAGCAGCAGGGGT+GGG + chr2_3:3423570-3423589 None:intergenic 60.0%
TTGGAAGCAGCAGCAGGGGT+GGG + chr2_3:3423570-3423589 None:intergenic 60.0%
GTTGGAAGCAGCAGCAGGGG+TGG + chr2_3:3423571-3423590 None:intergenic 65.0%
GTTGGAAGCAGCAGCAGGGG+TGG + chr2_3:3423571-3423590 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 3421498 3423892 3421498 ID=Msa0271910;Name=Msa0271910
chr2_3 mRNA 3421498 3423892 3421498 ID=Msa0271910-mRNA-1;Parent=Msa0271910;Name=Msa0271910-mRNA-1;_AED=0.01;_eAED=0.01;_QI=158|1|1|1|1|1|2|299|87
chr2_3 exon 3421498 3421979 3421498 ID=Msa0271910-mRNA-1:exon:1732;Parent=Msa0271910-mRNA-1
chr2_3 exon 3423654 3423892 3423654 ID=Msa0271910-mRNA-1:exon:1731;Parent=Msa0271910-mRNA-1
chr2_3 five_prime_UTR 3423735 3423892 3423735 ID=Msa0271910-mRNA-1:five_prime_utr;Parent=Msa0271910-mRNA-1
chr2_3 CDS 3423654 3423734 3423654 ID=Msa0271910-mRNA-1:cds;Parent=Msa0271910-mRNA-1
chr2_3 CDS 3421797 3421979 3421797 ID=Msa0271910-mRNA-1:cds;Parent=Msa0271910-mRNA-1
chr2_3 three_prime_UTR 3421498 3421796 3421498 ID=Msa0271910-mRNA-1:three_prime_utr;Parent=Msa0271910-mRNA-1
Gene Sequence

>Msa0271910

ATGAACGTGGAAGAAGAGGTTGGACGCCTAAAGGTAGAAATCAAGAGGCTTGGCAAGCTCCAATCAGATGGTTCTTACAAGGTTACATTTGGAACGCTATTTCATGATGACCAATGTGCAAATATATTTGAAGCACTTGTTGGGACACTAAGAGCAGCTAAAAAACGAAAAGTATTAACATATGAAGGTGAATTGCTGCTGCAAGGAGTCCATGACAATGTGGAAATCACTCTTAATCCCACCCCTGCTGCTGCTTCCAACTGA
Protein sequence

>Msa0271910

MNVEEEVGRLKVEIKRLGKLQSDGSYKVTFGTLFHDDQCANIFEALVGTLRAAKKRKVLTYEGELLLQGVHDNVEITLNPTPAAASN*