AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0310880

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0310880 MtrunA17_Chr2g0279651 96.552 87 3 0 1 87 1 87 1.39e-58 174
Msa0310880 MtrunA17_Chr4g0049791 84.615 78 12 0 1 78 1 78 1.82e-38 123
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0310880 AT4G33640.1 76.923 78 18 0 1 78 1 78 4.22e-40 127
Msa0310880 AT4G33640.2 76.923 78 18 0 1 78 67 144 1.34e-39 129

Find 33 sgRNAs with CRISPR-Local

Find 203 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTATTCAACGTCATTTAATA+TGG 0.239487 2_4:-5694317 Msa0310880:three_prime_UTR
GTTTCGAAATCTATAACTTT+AGG 0.262910 2_4:+5694222 None:intergenic
TATAAAACAAACACCCATAA+AGG 0.380566 2_4:+5694364 None:intergenic
TGTATTGTGAAGGTTACATT+TGG 0.382119 2_4:-5694609 Msa0310880:intron
ATATCTTCCACCTGATTGAT+AGG 0.386810 2_4:+5696231 None:intergenic
GCCTCTTGATTTCTACCTTT+AGG 0.396207 2_4:+5696120 None:intergenic
AATATATTTGAAGCACTTGT+TGG 0.411547 2_4:-5694558 Msa0310880:CDS
CCTTGTAAGAACCATCTGAT+TGG 0.444815 2_4:+5696087 None:intergenic
GTTCAGTTGGAAGCAGCAGC+AGG 0.447332 2_4:+5694435 None:intergenic
TAAATATCAGCCTATCAATC+AGG 0.457446 2_4:-5696241 Msa0310880:five_prime_UTR
ATATATTTGAAGCACTTGTT+GGG 0.466568 2_4:-5694557 Msa0310880:CDS
TTGGGTAGATAGTGTTCAGT+TGG 0.483464 2_4:+5694422 None:intergenic
CGAAAAGTATTAACATATGA+AGG 0.490626 2_4:-5694513 Msa0310880:CDS
TACAAATACAGCAAAACTGT+TGG 0.506470 2_4:+5694200 None:intergenic
CCAATCAGATGGTTCTTACA+AGG 0.510812 2_4:-5696087 Msa0310880:intron
AGAAGAGGTTGGACGCCTAA+AGG 0.519063 2_4:-5696135 Msa0310880:CDS
CTATAAAAGAAGTGTGTGAA+AGG 0.528769 2_4:-5694397 Msa0310880:three_prime_UTR
GAGTGATTTCCACATTGTCA+TGG 0.529569 2_4:+5694469 None:intergenic
CTTCAAATATATTTGCACAT+TGG 0.530146 2_4:+5694568 None:intergenic
AAGGTAGAAATCAAGAGGCT+TGG 0.545059 2_4:-5696116 Msa0310880:CDS
TTCAGTTGGAAGCAGCAGCA+GGG 0.553467 2_4:+5694436 None:intergenic
GTTGGAAGCAGCAGCAGGGG+TGG 0.553565 2_4:+5694440 None:intergenic
TTGGAAGCAGCAGCAGGGGT+GGG 0.565500 2_4:+5694441 None:intergenic
AAGCATGAACGTGGAAGAAG+AGG 0.576730 2_4:-5696150 Msa0310880:exon
CTTGGCAAGCTCCAATCAGA+TGG 0.580481 2_4:-5696098 Msa0310880:CDS
TATCTGAGAAGACAACAAGT+TGG 0.597136 2_4:-5696182 Msa0310880:five_prime_UTR
ATATCAGCCTATCAATCAGG+TGG 0.617592 2_4:-5696238 Msa0310880:five_prime_UTR
GCCTAAAGGTAGAAATCAAG+AGG 0.618558 2_4:-5696121 Msa0310880:CDS
ATGAACGTGGAAGAAGAGGT+TGG 0.620804 2_4:-5696146 Msa0310880:CDS
TCAGTTGGAAGCAGCAGCAG+GGG 0.624373 2_4:+5694437 None:intergenic
GAAGGTGAATTGCTGCTGCA+AGG 0.659800 2_4:-5694495 Msa0310880:CDS
AAGCAGAAGAAGCATGAACG+TGG 0.660179 2_4:-5696159 Msa0310880:exon
GCAAGGAGTCCATGACAATG+TGG 0.727047 2_4:-5694478 Msa0310880:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAATAAAATAAATAATA+AGG + chr2_4:5695701-5695720 None:intergenic 0.0%
!! AAAAAATAAAATAAATAATA+AGG + chr2_4:5695701-5695720 None:intergenic 0.0%
!!! TTATATATAGTTATTTTGAA+AGG + chr2_4:5694582-5694601 None:intergenic 10.0%
!!! TTATATATAGTTATTTTGAA+AGG + chr2_4:5694582-5694601 None:intergenic 10.0%
!!! AAATTTTAGAATTTATCACT+TGG - chr2_4:5694681-5694700 Msa0310880:intron 15.0%
!! AAGTTATAAATAGTTTCAAT+TGG + chr2_4:5695397-5695416 None:intergenic 15.0%
!! ATTGAAACTATTTATAACTT+TGG - chr2_4:5695397-5695416 Msa0310880:intron 15.0%
!! TTGAAACTATTTATAACTTT+GGG - chr2_4:5695398-5695417 Msa0310880:intron 15.0%
!!! ATAACATTTTCACTTAAAAT+TGG - chr2_4:5695472-5695491 Msa0310880:intron 15.0%
!!! AAATTTTAGAATTTATCACT+TGG - chr2_4:5694681-5694700 Msa0310880:intron 15.0%
!! AAGTTATAAATAGTTTCAAT+TGG + chr2_4:5695397-5695416 None:intergenic 15.0%
!! ATTGAAACTATTTATAACTT+TGG - chr2_4:5695397-5695416 Msa0310880:intron 15.0%
!! TTGAAACTATTTATAACTTT+GGG - chr2_4:5695398-5695417 Msa0310880:intron 15.0%
!!! ATAACATTTTCACTTAAAAT+TGG - chr2_4:5695472-5695491 Msa0310880:intron 15.0%
!!! GTGGAAGATATTTTAAATTA+AGG - chr2_4:5694223-5694242 Msa0310880:three_prime_UTR 20.0%
!! TGTATTATATTCTTAGTTCA+TGG - chr2_4:5694556-5694575 Msa0310880:CDS 20.0%
!! GATAATATGTGATTATAAGA+TGG + chr2_4:5694839-5694858 None:intergenic 20.0%
!! TTTAAATGTCTTTGTTAAGA+CGG + chr2_4:5694888-5694907 None:intergenic 20.0%
!! AGACATTTAAATTAAACACA+TGG - chr2_4:5694897-5694916 Msa0310880:intron 20.0%
!!! TAGTTTTTGAATCTTGATTT+AGG + chr2_4:5694988-5695007 None:intergenic 20.0%
!! TTATAAATAGTTTCAATTGG+TGG + chr2_4:5695394-5695413 None:intergenic 20.0%
!! TGAAACTATTTATAACTTTG+GGG - chr2_4:5695399-5695418 Msa0310880:intron 20.0%
!! TAAGTGAAAATGTTATGTAT+TGG + chr2_4:5695468-5695487 None:intergenic 20.0%
!! TCTGTCTATATACATAAATT+AGG - chr2_4:5695597-5695616 Msa0310880:intron 20.0%
!! CTGTCTATATACATAAATTA+GGG - chr2_4:5695598-5695617 Msa0310880:intron 20.0%
!! AAGGATAAAAATAACCTTTA+TGG - chr2_4:5696064-5696083 Msa0310880:intron 20.0%
!! AGGATAAAAATAACCTTTAT+GGG - chr2_4:5696065-5696084 Msa0310880:intron 20.0%
!!! GTGGAAGATATTTTAAATTA+AGG - chr2_4:5694223-5694242 Msa0310880:three_prime_UTR 20.0%
!! TGTATTATATTCTTAGTTCA+TGG - chr2_4:5694556-5694575 Msa0310880:CDS 20.0%
!! GATAATATGTGATTATAAGA+TGG + chr2_4:5694839-5694858 None:intergenic 20.0%
!! TTTAAATGTCTTTGTTAAGA+CGG + chr2_4:5694888-5694907 None:intergenic 20.0%
!! AGACATTTAAATTAAACACA+TGG - chr2_4:5694897-5694916 Msa0310880:intron 20.0%
!!! TAGTTTTTGAATCTTGATTT+AGG + chr2_4:5694988-5695007 None:intergenic 20.0%
!! TTATAAATAGTTTCAATTGG+TGG + chr2_4:5695394-5695413 None:intergenic 20.0%
!! TGAAACTATTTATAACTTTG+GGG - chr2_4:5695399-5695418 Msa0310880:intron 20.0%
!! TAAGTGAAAATGTTATGTAT+TGG + chr2_4:5695468-5695487 None:intergenic 20.0%
!! TCTGTCTATATACATAAATT+AGG - chr2_4:5695597-5695616 Msa0310880:intron 20.0%
!! CTGTCTATATACATAAATTA+GGG - chr2_4:5695598-5695617 Msa0310880:intron 20.0%
!! AAGGATAAAAATAACCTTTA+TGG - chr2_4:5696064-5696083 Msa0310880:intron 20.0%
!! AGGATAAAAATAACCTTTAT+GGG - chr2_4:5696065-5696084 Msa0310880:intron 20.0%
!! TTCAATTTTCAGATCTGTAT+AGG - chr2_4:5694423-5694442 Msa0310880:exon 25.0%
!! TCAATTTTCAGATCTGTATA+GGG - chr2_4:5694424-5694443 Msa0310880:exon 25.0%
! ATCTATCACATAAAGCAAAT+TGG + chr2_4:5694454-5694473 None:intergenic 25.0%
! GTGATGTTATTGATGTATAA+AGG + chr2_4:5694817-5694836 None:intergenic 25.0%
! CTATTAACTCTGATATTTCT+AGG + chr2_4:5694956-5694975 None:intergenic 25.0%
! GAAATATCAGAGTTAATAGA+TGG - chr2_4:5694957-5694976 Msa0310880:intron 25.0%
!!! TTTGTGATTTGAAGTTTTAG+TGG + chr2_4:5695015-5695034 None:intergenic 25.0%
!! TAGCTAAACTCAAATTTGTT+GGG + chr2_4:5695152-5695171 None:intergenic 25.0%
! TAAACAAATTCAGTCTGAAT+TGG + chr2_4:5695244-5695263 None:intergenic 25.0%
!! TTTTGTGAAGATTAAAGTGA+AGG + chr2_4:5695330-5695349 None:intergenic 25.0%
!! GGTACATTTTGAATGTAATT+TGG + chr2_4:5695444-5695463 None:intergenic 25.0%
! CTTCAAATATATTTGCACAT+TGG + chr2_4:5695877-5695896 None:intergenic 25.0%
!! AATATATTTGAAGCACTTGT+TGG - chr2_4:5695884-5695903 Msa0310880:intron 25.0%
!! ATATATTTGAAGCACTTGTT+GGG - chr2_4:5695885-5695904 Msa0310880:intron 25.0%
!!! CGAAAAGTATTAACATATGA+AGG - chr2_4:5695929-5695948 Msa0310880:intron 25.0%
!! CACACTTCTTTTATAGTTTT+GGG + chr2_4:5696041-5696060 None:intergenic 25.0%
!! ACACACTTCTTTTATAGTTT+TGG + chr2_4:5696042-5696061 None:intergenic 25.0%
! TATAAAACAAACACCCATAA+AGG + chr2_4:5696081-5696100 None:intergenic 25.0%
! CTATTCAACGTCATTTAATA+TGG - chr2_4:5696125-5696144 Msa0310880:CDS 25.0%
!!! TTTTCTTGATGACAAGATTT+TGG + chr2_4:5696169-5696188 None:intergenic 25.0%
!!! CACAATTTTCTTTTTTTGAG+AGG + chr2_4:5696199-5696218 None:intergenic 25.0%
! GTTTCGAAATCTATAACTTT+AGG + chr2_4:5696223-5696242 None:intergenic 25.0%
! GTGTTACATCGTTAAAAATT+TGG + chr2_4:5694140-5694159 None:intergenic 25.0%
!! TTCAATTTTCAGATCTGTAT+AGG - chr2_4:5694423-5694442 Msa0310880:exon 25.0%
!! TCAATTTTCAGATCTGTATA+GGG - chr2_4:5694424-5694443 Msa0310880:exon 25.0%
! ATCTATCACATAAAGCAAAT+TGG + chr2_4:5694454-5694473 None:intergenic 25.0%
! GTGATGTTATTGATGTATAA+AGG + chr2_4:5694817-5694836 None:intergenic 25.0%
! CTATTAACTCTGATATTTCT+AGG + chr2_4:5694956-5694975 None:intergenic 25.0%
! GAAATATCAGAGTTAATAGA+TGG - chr2_4:5694957-5694976 Msa0310880:intron 25.0%
!!! TTTGTGATTTGAAGTTTTAG+TGG + chr2_4:5695015-5695034 None:intergenic 25.0%
!! TAGCTAAACTCAAATTTGTT+GGG + chr2_4:5695152-5695171 None:intergenic 25.0%
! TAAACAAATTCAGTCTGAAT+TGG + chr2_4:5695244-5695263 None:intergenic 25.0%
!! TTTTGTGAAGATTAAAGTGA+AGG + chr2_4:5695330-5695349 None:intergenic 25.0%
!! GGTACATTTTGAATGTAATT+TGG + chr2_4:5695444-5695463 None:intergenic 25.0%
! CTTCAAATATATTTGCACAT+TGG + chr2_4:5695877-5695896 None:intergenic 25.0%
!! AATATATTTGAAGCACTTGT+TGG - chr2_4:5695884-5695903 Msa0310880:intron 25.0%
!! ATATATTTGAAGCACTTGTT+GGG - chr2_4:5695885-5695904 Msa0310880:intron 25.0%
!!! CGAAAAGTATTAACATATGA+AGG - chr2_4:5695929-5695948 Msa0310880:intron 25.0%
!! CACACTTCTTTTATAGTTTT+GGG + chr2_4:5696041-5696060 None:intergenic 25.0%
!! ACACACTTCTTTTATAGTTT+TGG + chr2_4:5696042-5696061 None:intergenic 25.0%
! TATAAAACAAACACCCATAA+AGG + chr2_4:5696081-5696100 None:intergenic 25.0%
! CTATTCAACGTCATTTAATA+TGG - chr2_4:5696125-5696144 Msa0310880:CDS 25.0%
!!! TTTTCTTGATGACAAGATTT+TGG + chr2_4:5696169-5696188 None:intergenic 25.0%
!!! CACAATTTTCTTTTTTTGAG+AGG + chr2_4:5696199-5696218 None:intergenic 25.0%
! GTTTCGAAATCTATAACTTT+AGG + chr2_4:5696223-5696242 None:intergenic 25.0%
TAAATATCAGCCTATCAATC+AGG - chr2_4:5694201-5694220 Msa0310880:three_prime_UTR 30.0%
AACAGTAACAAAAGTAACGA+TGG - chr2_4:5694710-5694729 Msa0310880:intron 30.0%
GATTTAATAGTCCACAAAAC+TGG + chr2_4:5694923-5694942 None:intergenic 30.0%
GTAGCTAAACTCAAATTTGT+TGG + chr2_4:5695153-5695172 None:intergenic 30.0%
AGTTTAGCTACTCATATACA+TGG - chr2_4:5695162-5695181 Msa0310880:intron 30.0%
GTGAAAATGTTATGTATTGG+TGG + chr2_4:5695465-5695484 None:intergenic 30.0%
TCATGTATCAGAAGAAACTA+TGG + chr2_4:5695529-5695548 None:intergenic 30.0%
AGAGAACATTGGAAGTAAAT+GGG + chr2_4:5695624-5695643 None:intergenic 30.0%
AAGATGAAAACAGAGAACAT+TGG + chr2_4:5695635-5695654 None:intergenic 30.0%
TGAGAAGAATGAACAAAATG+TGG - chr2_4:5695803-5695822 Msa0310880:intron 30.0%
GAGAAGAATGAACAAAATGT+GGG - chr2_4:5695804-5695823 Msa0310880:intron 30.0%
TGTATTGTGAAGGTTACATT+TGG - chr2_4:5695833-5695852 Msa0310880:intron 30.0%
! CTATAAAAGAAGTGTGTGAA+AGG - chr2_4:5696045-5696064 Msa0310880:intron 30.0%
TACAAATACAGCAAAACTGT+TGG + chr2_4:5696245-5696264 None:intergenic 30.0%
!! CAGTTTTGCTGTATTTGTAA+TGG - chr2_4:5696246-5696265 Msa0310880:five_prime_UTR 30.0%
TAAATATCAGCCTATCAATC+AGG - chr2_4:5694201-5694220 Msa0310880:three_prime_UTR 30.0%
AACAGTAACAAAAGTAACGA+TGG - chr2_4:5694710-5694729 Msa0310880:intron 30.0%
GATTTAATAGTCCACAAAAC+TGG + chr2_4:5694923-5694942 None:intergenic 30.0%
GTAGCTAAACTCAAATTTGT+TGG + chr2_4:5695153-5695172 None:intergenic 30.0%
AGTTTAGCTACTCATATACA+TGG - chr2_4:5695162-5695181 Msa0310880:intron 30.0%
GTGAAAATGTTATGTATTGG+TGG + chr2_4:5695465-5695484 None:intergenic 30.0%
TCATGTATCAGAAGAAACTA+TGG + chr2_4:5695529-5695548 None:intergenic 30.0%
AGAGAACATTGGAAGTAAAT+GGG + chr2_4:5695624-5695643 None:intergenic 30.0%
AAGATGAAAACAGAGAACAT+TGG + chr2_4:5695635-5695654 None:intergenic 30.0%
TGAGAAGAATGAACAAAATG+TGG - chr2_4:5695803-5695822 Msa0310880:intron 30.0%
GAGAAGAATGAACAAAATGT+GGG - chr2_4:5695804-5695823 Msa0310880:intron 30.0%
TGTATTGTGAAGGTTACATT+TGG - chr2_4:5695833-5695852 Msa0310880:intron 30.0%
! CTATAAAAGAAGTGTGTGAA+AGG - chr2_4:5696045-5696064 Msa0310880:intron 30.0%
TACAAATACAGCAAAACTGT+TGG + chr2_4:5696245-5696264 None:intergenic 30.0%
!! CAGTTTTGCTGTATTTGTAA+TGG - chr2_4:5696246-5696265 Msa0310880:five_prime_UTR 30.0%
ATATCTTCCACCTGATTGAT+AGG + chr2_4:5694214-5694233 None:intergenic 35.0%
TATCTGAGAAGACAACAAGT+TGG - chr2_4:5694260-5694279 Msa0310880:three_prime_UTR 35.0%
GATATACTTGTCTATGCTTG+TGG - chr2_4:5694471-5694490 Msa0310880:CDS 35.0%
TCTGCATCTTGACTTCATAT+AGG - chr2_4:5694495-5694514 Msa0310880:CDS 35.0%
CTTAATCTTGCATCAAACTG+TGG - chr2_4:5694523-5694542 Msa0310880:CDS 35.0%
AACTTTGTTGAACAGCTTGA+AGG + chr2_4:5694650-5694669 None:intergenic 35.0%
ACATGTATCTTTGCTTGAGA+AGG + chr2_4:5694793-5694812 None:intergenic 35.0%
ATCATACCGTATAACAAGCA+TGG - chr2_4:5695034-5695053 Msa0310880:intron 35.0%
!! TGTTCTCCATGCTTGTTATA+CGG + chr2_4:5695043-5695062 None:intergenic 35.0%
! GACTGAATTTGTTTAGCACT+TGG - chr2_4:5695249-5695268 Msa0310880:intron 35.0%
AAGCGAATTTGTGTTTGCTT+TGG - chr2_4:5695281-5695300 Msa0310880:intron 35.0%
ATTAAAGTGAAGGAATGAGC+AGG + chr2_4:5695320-5695339 None:intergenic 35.0%
TGTATCAGAAGAAACTATGG+AGG + chr2_4:5695526-5695545 None:intergenic 35.0%
TAGACAGAAGAAACTATCTC+AGG + chr2_4:5695585-5695604 None:intergenic 35.0%
CAGAGAACATTGGAAGTAAA+TGG + chr2_4:5695625-5695644 None:intergenic 35.0%
AGAATGAACAAAATGTGGGT+TGG - chr2_4:5695808-5695827 Msa0310880:intron 35.0%
ATATCTTCCACCTGATTGAT+AGG + chr2_4:5694214-5694233 None:intergenic 35.0%
TATCTGAGAAGACAACAAGT+TGG - chr2_4:5694260-5694279 Msa0310880:three_prime_UTR 35.0%
GATATACTTGTCTATGCTTG+TGG - chr2_4:5694471-5694490 Msa0310880:CDS 35.0%
TCTGCATCTTGACTTCATAT+AGG - chr2_4:5694495-5694514 Msa0310880:CDS 35.0%
CTTAATCTTGCATCAAACTG+TGG - chr2_4:5694523-5694542 Msa0310880:CDS 35.0%
AACTTTGTTGAACAGCTTGA+AGG + chr2_4:5694650-5694669 None:intergenic 35.0%
ACATGTATCTTTGCTTGAGA+AGG + chr2_4:5694793-5694812 None:intergenic 35.0%
ATCATACCGTATAACAAGCA+TGG - chr2_4:5695034-5695053 Msa0310880:intron 35.0%
!! TGTTCTCCATGCTTGTTATA+CGG + chr2_4:5695043-5695062 None:intergenic 35.0%
! GACTGAATTTGTTTAGCACT+TGG - chr2_4:5695249-5695268 Msa0310880:intron 35.0%
AAGCGAATTTGTGTTTGCTT+TGG - chr2_4:5695281-5695300 Msa0310880:intron 35.0%
ATTAAAGTGAAGGAATGAGC+AGG + chr2_4:5695320-5695339 None:intergenic 35.0%
TGTATCAGAAGAAACTATGG+AGG + chr2_4:5695526-5695545 None:intergenic 35.0%
TAGACAGAAGAAACTATCTC+AGG + chr2_4:5695585-5695604 None:intergenic 35.0%
CAGAGAACATTGGAAGTAAA+TGG + chr2_4:5695625-5695644 None:intergenic 35.0%
AGAATGAACAAAATGTGGGT+TGG - chr2_4:5695808-5695827 Msa0310880:intron 35.0%
ATATCAGCCTATCAATCAGG+TGG - chr2_4:5694204-5694223 Msa0310880:three_prime_UTR 40.0%
GCCTAAAGGTAGAAATCAAG+AGG - chr2_4:5694321-5694340 Msa0310880:three_prime_UTR 40.0%
GCCTCTTGATTTCTACCTTT+AGG + chr2_4:5694325-5694344 None:intergenic 40.0%
AAGGTAGAAATCAAGAGGCT+TGG - chr2_4:5694326-5694345 Msa0310880:three_prime_UTR 40.0%
CCTTGTAAGAACCATCTGAT+TGG + chr2_4:5694358-5694377 None:intergenic 40.0%
CCAATCAGATGGTTCTTACA+AGG - chr2_4:5694355-5694374 Msa0310880:three_prime_UTR 40.0%
AGAGAAGAGAAGAGAAGAGA+AGG + chr2_4:5694393-5694412 None:intergenic 40.0%
! TAAACACATGGCCAGTTTTG+TGG - chr2_4:5694909-5694928 Msa0310880:intron 40.0%
AGTCTGAATTGGAGCTCATT+TGG + chr2_4:5695233-5695252 None:intergenic 40.0%
CTATGGAGGTAAAAAGTTGC+TGG + chr2_4:5695512-5695531 None:intergenic 40.0%
ATACATGACTGCTTGATCAC+AGG - chr2_4:5695541-5695560 Msa0310880:intron 40.0%
! TGGGTTGGATTGTATTGTGA+AGG - chr2_4:5695823-5695842 Msa0310880:intron 40.0%
GAGTGATTTCCACATTGTCA+TGG + chr2_4:5695976-5695995 None:intergenic 40.0%
TTGGGTAGATAGTGTTCAGT+TGG + chr2_4:5696023-5696042 None:intergenic 40.0%
ATATCAGCCTATCAATCAGG+TGG - chr2_4:5694204-5694223 Msa0310880:three_prime_UTR 40.0%
GCCTAAAGGTAGAAATCAAG+AGG - chr2_4:5694321-5694340 Msa0310880:three_prime_UTR 40.0%
GCCTCTTGATTTCTACCTTT+AGG + chr2_4:5694325-5694344 None:intergenic 40.0%
AAGGTAGAAATCAAGAGGCT+TGG - chr2_4:5694326-5694345 Msa0310880:three_prime_UTR 40.0%
CCTTGTAAGAACCATCTGAT+TGG + chr2_4:5694358-5694377 None:intergenic 40.0%
CCAATCAGATGGTTCTTACA+AGG - chr2_4:5694355-5694374 Msa0310880:three_prime_UTR 40.0%
AGAGAAGAGAAGAGAAGAGA+AGG + chr2_4:5694393-5694412 None:intergenic 40.0%
! TAAACACATGGCCAGTTTTG+TGG - chr2_4:5694909-5694928 Msa0310880:intron 40.0%
AGTCTGAATTGGAGCTCATT+TGG + chr2_4:5695233-5695252 None:intergenic 40.0%
CTATGGAGGTAAAAAGTTGC+TGG + chr2_4:5695512-5695531 None:intergenic 40.0%
ATACATGACTGCTTGATCAC+AGG - chr2_4:5695541-5695560 Msa0310880:intron 40.0%
! TGGGTTGGATTGTATTGTGA+AGG - chr2_4:5695823-5695842 Msa0310880:intron 40.0%
GAGTGATTTCCACATTGTCA+TGG + chr2_4:5695976-5695995 None:intergenic 40.0%
TTGGGTAGATAGTGTTCAGT+TGG + chr2_4:5696023-5696042 None:intergenic 40.0%
! AAGCAGAAGAAGCATGAACG+TGG - chr2_4:5694283-5694302 Msa0310880:three_prime_UTR 45.0%
AAGCATGAACGTGGAAGAAG+AGG - chr2_4:5694292-5694311 Msa0310880:three_prime_UTR 45.0%
ATGAACGTGGAAGAAGAGGT+TGG - chr2_4:5694296-5694315 Msa0310880:three_prime_UTR 45.0%
! AAGCAGAAGAAGCATGAACG+TGG - chr2_4:5694283-5694302 Msa0310880:three_prime_UTR 45.0%
AAGCATGAACGTGGAAGAAG+AGG - chr2_4:5694292-5694311 Msa0310880:three_prime_UTR 45.0%
ATGAACGTGGAAGAAGAGGT+TGG - chr2_4:5694296-5694315 Msa0310880:three_prime_UTR 45.0%
!! ATAAAATAATAAAAAAAGAT+TGG - chr2_4:5696295-5696314 Msa0310880:five_prime_UTR 5.0%
!! TAAAATAATAAAAAAAGATT+GGG - chr2_4:5696296-5696315 Msa0310880:five_prime_UTR 5.0%
!! ATAAAATAATAAAAAAAGAT+TGG - chr2_4:5696295-5696314 Msa0310880:five_prime_UTR 5.0%
!! TAAAATAATAAAAAAAGATT+GGG - chr2_4:5696296-5696315 Msa0310880:five_prime_UTR 5.0%
AGAAGAGGTTGGACGCCTAA+AGG - chr2_4:5694307-5694326 Msa0310880:three_prime_UTR 50.0%
CTTGGCAAGCTCCAATCAGA+TGG - chr2_4:5694344-5694363 Msa0310880:three_prime_UTR 50.0%
GAAGGTGAATTGCTGCTGCA+AGG - chr2_4:5695947-5695966 Msa0310880:intron 50.0%
GCAAGGAGTCCATGACAATG+TGG - chr2_4:5695964-5695983 Msa0310880:intron 50.0%
TTCAGTTGGAAGCAGCAGCA+GGG + chr2_4:5696009-5696028 None:intergenic 50.0%
AGAAGAGGTTGGACGCCTAA+AGG - chr2_4:5694307-5694326 Msa0310880:three_prime_UTR 50.0%
CTTGGCAAGCTCCAATCAGA+TGG - chr2_4:5694344-5694363 Msa0310880:three_prime_UTR 50.0%
GAAGGTGAATTGCTGCTGCA+AGG - chr2_4:5695947-5695966 Msa0310880:intron 50.0%
GCAAGGAGTCCATGACAATG+TGG - chr2_4:5695964-5695983 Msa0310880:intron 50.0%
TTCAGTTGGAAGCAGCAGCA+GGG + chr2_4:5696009-5696028 None:intergenic 50.0%
TCAGTTGGAAGCAGCAGCAG+GGG + chr2_4:5696008-5696027 None:intergenic 55.0%
GTTCAGTTGGAAGCAGCAGC+AGG + chr2_4:5696010-5696029 None:intergenic 55.0%
TCAGTTGGAAGCAGCAGCAG+GGG + chr2_4:5696008-5696027 None:intergenic 55.0%
GTTCAGTTGGAAGCAGCAGC+AGG + chr2_4:5696010-5696029 None:intergenic 55.0%
TTGGAAGCAGCAGCAGGGGT+GGG + chr2_4:5696004-5696023 None:intergenic 60.0%
TTGGAAGCAGCAGCAGGGGT+GGG + chr2_4:5696004-5696023 None:intergenic 60.0%
GTTGGAAGCAGCAGCAGGGG+TGG + chr2_4:5696005-5696024 None:intergenic 65.0%
GTTGGAAGCAGCAGCAGGGG+TGG + chr2_4:5696005-5696024 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 5694138 5696326 5694138 ID=Msa0310880;Name=Msa0310880
chr2_4 mRNA 5694138 5696326 5694138 ID=Msa0310880-mRNA-1;Parent=Msa0310880;Name=Msa0310880-mRNA-1;_AED=0.03;_eAED=0.03;_QI=158|1|1|1|1|1|2|299|87
chr2_4 exon 5694138 5694619 5694138 ID=Msa0310880-mRNA-1:exon:2173;Parent=Msa0310880-mRNA-1
chr2_4 exon 5696088 5696326 5696088 ID=Msa0310880-mRNA-1:exon:2172;Parent=Msa0310880-mRNA-1
chr2_4 five_prime_UTR 5696169 5696326 5696169 ID=Msa0310880-mRNA-1:five_prime_utr;Parent=Msa0310880-mRNA-1
chr2_4 CDS 5696088 5696168 5696088 ID=Msa0310880-mRNA-1:cds;Parent=Msa0310880-mRNA-1
chr2_4 CDS 5694437 5694619 5694437 ID=Msa0310880-mRNA-1:cds;Parent=Msa0310880-mRNA-1
chr2_4 three_prime_UTR 5694138 5694436 5694138 ID=Msa0310880-mRNA-1:three_prime_utr;Parent=Msa0310880-mRNA-1
Gene Sequence

>Msa0310880

ATGAACGTGGAAGAAGAGGTTGGACGCCTAAAGGTAGAAATCAAGAGGCTTGGCAAGCTCCAATCAGATGGTTCTTACAAGGTTACATTTGGAACGCTATTTCATGATGACCAATGTGCAAATATATTTGAAGCACTTGTTGGGACACTAAGAGCAGCTAAAAAACGAAAAGTATTAACATATGAAGGTGAATTGCTGCTGCAAGGAGTCCATGACAATGTGGAAATCACTCTTAATCCCACCCCTGCTGCTGCTTCCAACTGA
Protein sequence

>Msa0310880

MNVEEEVGRLKVEIKRLGKLQSDGSYKVTFGTLFHDDQCANIFEALVGTLRAAKKRKVLTYEGELLLQGVHDNVEITLNPTPAAASN*