AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0236990


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0236990 MtrunA17_Chr2g0286171 97.170 212 5 1 1 211 1 212 3.34e-148 410
Msa0236990 MtrunA17_Chr3g0115141 84.360 211 32 1 1 211 1 210 4.13e-117 332
Msa0236990 MtrunA17_Chr4g0003361 64.623 212 65 5 3 211 1 205 6.87e-85 250
Msa0236990 MtrunA17_Chr8g0343934 55.607 214 87 4 1 211 1 209 7.47e-78 232
Msa0236990 MtrunA17_Chr8g0343954 54.206 214 90 3 1 211 1 209 5.89e-77 230
Msa0236990 MtrunA17_Chr8g0344031 57.944 214 84 4 1 211 1 211 2.61e-75 226
Msa0236990 MtrunA17_Chr8g0344041 57.674 215 81 5 1 211 5 213 5.60e-75 225
Msa0236990 MtrunA17_Chr8g0343961 53.738 214 91 4 1 211 1 209 1.21e-73 221
Msa0236990 MtrunA17_Chr2g0301611 53.738 214 91 4 1 211 1 209 4.37e-73 220
Msa0236990 MtrunA17_Chr8g0344021 55.349 215 89 4 1 211 1 212 1.19e-72 219
Msa0236990 MtrunA17_Chr2g0313961 52.113 213 94 4 1 210 1 208 4.83e-71 215
Msa0236990 MtrunA17_Chr2g0302171 52.778 216 90 4 1 211 1 209 1.13e-70 214
Msa0236990 MtrunA17_Chr7g0217811 57.447 188 76 2 27 211 23 209 9.30e-70 211
Msa0236990 MtrunA17_Chr2g0297231 52.381 210 92 4 1 207 1 205 1.25e-69 211
Msa0236990 MtrunA17_Chr3g0084531 52.358 212 95 3 3 211 1 209 2.33e-69 211
Msa0236990 MtrunA17_Chr2g0302541 55.208 192 82 2 23 211 19 209 4.58e-69 210
Msa0236990 MtrunA17_Chr2g0297241 50.698 215 94 4 1 210 1 208 1.16e-68 209
Msa0236990 MtrunA17_Chr4g0012651 55.851 188 79 2 27 211 23 209 8.15e-68 207
Msa0236990 MtrunA17_Chr3g0084521 51.402 214 95 4 1 211 1 208 1.93e-67 206
Msa0236990 MtrunA17_Chr5g0411961 51.402 214 96 4 1 211 1 209 3.75e-67 205
Msa0236990 MtrunA17_Chr3g0084511 50.935 214 96 4 1 211 1 208 7.15e-67 204
Msa0236990 MtrunA17_Chr8g0344051 50.000 216 99 5 1 211 1 212 3.07e-65 200
Msa0236990 MtrunA17_Chr8g0343911 46.078 204 104 4 13 211 6 208 4.51e-59 184
Msa0236990 MtrunA17_Chr8g0343921 46.602 206 104 5 11 211 3 207 2.49e-56 177
Msa0236990 MtrunA17_Chr2g0300561 42.130 216 114 5 1 211 1 210 1.30e-51 166
Msa0236990 MtrunA17_Chr2g0300581 41.204 216 116 5 1 211 1 210 2.81e-50 162
Msa0236990 MtrunA17_Chr2g0300591 41.204 216 116 5 1 211 1 210 7.70e-50 161
Msa0236990 MtrunA17_Chr3g0084551 43.192 213 107 5 3 211 1 203 7.97e-47 154
Msa0236990 MtrunA17_Chr1g0189841 37.327 217 126 4 1 207 1 217 1.48e-36 127
Msa0236990 MtrunA17_Chr6g0449761 33.641 217 132 6 1 207 1 215 4.78e-28 106
Msa0236990 MtrunA17_Chr6g0449771 33.641 217 132 6 1 207 1 215 6.35e-28 105
Msa0236990 MtrunA17_Chr1g0193421 33.511 188 117 5 27 207 29 215 1.08e-27 104
Msa0236990 MtrunA17_Chr6g0449751 34.259 216 132 5 1 207 1 215 1.55e-27 103
Msa0236990 MtrunA17_Chr6g0449731 33.641 217 132 6 1 207 1 215 2.11e-27 103
Msa0236990 MtrunA17_Chr1g0178711 31.455 213 135 5 6 207 9 221 8.04e-27 102
Msa0236990 MtrunA17_Chr4g0007041 33.180 217 132 6 1 206 1 215 6.57e-26 100
Msa0236990 MtrunA17_Chr4g0007051 33.180 217 132 6 1 206 1 215 8.94e-26 99.8
Msa0236990 MtrunA17_Chr4g0007031 33.180 217 132 6 1 206 1 215 1.15e-25 99.4
Msa0236990 MtrunA17_Chr5g0419971 33.180 217 132 6 1 206 1 215 1.15e-25 99.4
Msa0236990 MtrunA17_Chr5g0419981 33.180 217 132 6 1 206 1 215 1.15e-25 99.4
Msa0236990 MtrunA17_Chr2g0292831 34.536 194 114 5 23 207 23 212 2.66e-25 98.2
Msa0236990 MtrunA17_Chr1g0193671 30.952 210 137 5 4 206 5 213 2.80e-25 98.2
Msa0236990 MtrunA17_Chr8g0335851 34.320 169 94 3 42 206 47 202 5.17e-25 97.4
Msa0236990 MtrunA17_Chr2g0285871 32.719 217 133 6 1 206 1 215 5.23e-25 97.4
Msa0236990 MtrunA17_Chr2g0292901 32.663 199 121 5 18 207 19 213 5.24e-25 97.4
Msa0236990 MtrunA17_Chr1g0210761 32.258 186 113 7 28 207 29 207 5.33e-25 97.4
Msa0236990 MtrunA17_Chr6g0449791 35.955 178 105 4 1 170 1 177 9.15e-25 96.7
Msa0236990 MtrunA17_Chr1g0156131 32.474 194 115 5 23 207 23 209 3.06e-23 92.8
Msa0236990 MtrunA17_Chr5g0414971 30.526 190 123 6 28 209 33 221 5.78e-23 92.0
Msa0236990 MtrunA17_Chr3g0084501 46.429 112 48 4 1 107 1 105 6.11e-23 89.0
Msa0236990 MtrunA17_Chr7g0271211 31.915 188 120 5 27 207 25 211 6.04e-22 89.4
Msa0236990 MtrunA17_Chr2g0321591 35.404 161 98 4 52 207 45 204 8.45e-22 89.0
Msa0236990 MtrunA17_Chr3g0130241 33.880 183 105 5 1 170 63 242 9.46e-22 90.5
Msa0236990 MtrunA17_Chr4g0026201 36.691 139 86 2 71 207 67 205 1.25e-21 88.2
Msa0236990 MtrunA17_Chr2g0321581 34.783 161 99 4 52 207 45 204 3.46e-21 87.0
Msa0236990 MtrunA17_Chr4g0026191 35.971 139 87 2 71 207 67 205 4.89e-21 86.7
Msa0236990 MtrunA17_Chr2g0292861 33.140 172 105 5 46 207 7 178 1.44e-20 84.7
Msa0236990 MtrunA17_Chr2g0321601 35.000 140 87 4 71 207 65 203 5.51e-20 84.0
Msa0236990 MtrunA17_Chr6g0449781 31.383 188 120 5 28 207 28 214 1.90e-19 82.8
Msa0236990 MtrunA17_Chr2g0292841 31.977 172 107 5 46 207 7 178 3.57e-18 78.6
Msa0236990 MtrunA17_Chr2g0327651 38.614 101 60 1 47 145 7 107 8.64e-17 73.2
Msa0236990 MtrunA17_Chr2g0292911 37.607 117 66 3 46 155 7 123 6.89e-16 71.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0236990 AT1G72610.1 47.423 194 96 4 23 211 16 208 1.58e-59 186
Msa0236990 AT5G20630.1 43.192 213 113 5 3 210 1 210 1.31e-50 163
Msa0236990 AT5G39160.3 34.703 219 127 6 1 207 1 215 1.15e-34 122
Msa0236990 AT5G39110.1 36.916 214 124 5 6 208 8 221 5.14e-34 121
Msa0236990 AT5G39190.1 34.247 219 132 5 1 207 1 219 1.07e-33 120
Msa0236990 AT5G39160.1 34.247 219 132 5 1 207 1 219 1.08e-33 120
Msa0236990 AT5G39120.1 35.714 224 122 7 1 208 3 220 1.19e-33 120
Msa0236990 AT5G39130.1 33.333 219 134 5 1 207 1 219 6.87e-33 118
Msa0236990 AT5G39180.1 35.268 224 123 7 1 208 3 220 1.74e-32 117
Msa0236990 AT5G39150.1 35.268 224 123 7 1 208 3 220 5.01e-32 116
Msa0236990 AT4G14630.1 34.597 211 127 5 8 207 12 222 2.30e-31 114
Msa0236990 AT3G05950.1 35.714 210 121 5 12 207 12 221 8.90e-31 113
Msa0236990 AT1G02335.1 31.429 210 134 6 6 207 8 215 1.31e-30 112
Msa0236990 AT3G04190.1 34.021 194 118 4 24 207 28 221 1.71e-30 112
Msa0236990 AT5G38960.1 36.923 195 112 5 24 207 28 222 9.26e-30 110
Msa0236990 AT5G39160.2 31.628 215 121 4 1 207 1 197 1.26e-29 109
Msa0236990 AT5G39190.2 31.628 215 121 4 1 207 1 197 1.35e-29 109
Msa0236990 AT1G09560.1 35.678 199 115 6 22 208 19 216 1.97e-29 109
Msa0236990 AT5G26700.1 33.173 208 129 6 5 207 7 209 5.46e-29 108
Msa0236990 AT3G04200.1 33.953 215 122 5 8 207 13 222 5.66e-29 108
Msa0236990 AT1G18970.1 35.149 202 125 5 11 207 18 218 1.16e-28 107
Msa0236990 AT3G05930.1 33.178 214 131 7 3 207 5 215 1.97e-28 106
Msa0236990 AT1G18980.1 37.097 186 109 6 28 207 35 218 2.06e-28 106
Msa0236990 AT3G04180.1 33.333 201 110 5 24 207 28 221 2.48e-28 106
Msa0236990 AT3G04150.1 30.769 195 125 4 27 211 31 225 1.42e-27 104
Msa0236990 AT3G04170.1 31.905 210 130 5 8 207 12 218 6.46e-27 103
Msa0236990 AT5G38940.1 35.385 195 116 5 24 208 28 222 7.84e-26 100
Msa0236990 AT5G38940.2 35.385 195 116 5 24 208 52 246 1.13e-25 100
Msa0236990 AT3G62020.1 31.746 189 120 6 27 207 28 215 7.45e-25 97.4
Msa0236990 AT3G10080.1 33.511 188 117 3 28 207 32 219 7.56e-25 97.8
Msa0236990 AT3G04150.2 30.682 176 112 4 46 211 63 238 9.72e-25 97.8
Msa0236990 AT3G10080.2 33.511 188 117 3 28 207 103 290 1.55e-24 98.6
Msa0236990 AT5G38910.1 33.649 211 130 5 7 207 9 219 1.85e-24 96.7
Msa0236990 AT5G61750.1 34.503 171 104 5 6 170 5 173 1.97e-24 96.3
Msa0236990 AT3G62020.2 32.184 174 110 5 42 207 13 186 2.67e-24 95.5
Msa0236990 AT5G38930.1 33.846 195 119 5 24 208 28 222 5.94e-23 92.4
Msa0236990 AT1G74820.1 31.776 214 133 6 6 207 13 225 6.04e-22 90.1
Msa0236990 AT1G10460.1 29.897 194 125 7 27 209 24 217 2.06e-20 85.9
Msa0236990 AT5G38910.2 29.327 208 106 5 7 207 9 182 5.47e-13 65.1

Find 37 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAGCTTAAGGCTAAGTTTA+AGG 0.120944 2_2:-10078940 Msa0236990:CDS
TCTTAAGCCTACTGATATTA+TGG 0.242052 2_2:-10079141 Msa0236990:CDS
GTGAATGATTTCGTGTTATC+TGG 0.259765 2_2:-10079392 Msa0236990:CDS
ACAAAATGCAATAGTCCTTT+TGG 0.270307 2_2:+10079113 None:intergenic
AAAGCTTTGTTTAAGCTTCC+AGG 0.294960 2_2:+10079362 None:intergenic
TTTCTTGATTCTGTATTGTT+TGG 0.320257 2_2:-10079023 Msa0236990:CDS
AAGCTTAAGGCTAAGTTTAA+GGG 0.375783 2_2:-10078939 Msa0236990:CDS
GATTTCGTGTTATCTGGCTT+TGG 0.389146 2_2:-10079386 Msa0236990:CDS
TAATTCAGTAACTGATGCTT+TGG 0.397550 2_2:+10079325 None:intergenic
TTCACAGATCAAGAAGCTTA+AGG 0.430319 2_2:-10078952 Msa0236990:CDS
GTGTTATCTGGCTTTGGACC+TGG 0.438197 2_2:-10079380 Msa0236990:CDS
TGCATTGGAACAGATCCATT+TGG 0.439588 2_2:+10079254 None:intergenic
ATTAGTAATTCAGTTGCATC+AGG 0.439691 2_2:+10079224 None:intergenic
AGAGTAGACATTGGTCCAAA+TGG 0.456817 2_2:-10079269 Msa0236990:CDS
CTTTGACATAAACTTCTGTA+AGG 0.459348 2_2:+10079165 None:intergenic
TTTGTAGTTAACTCTGGTGC+TGG 0.460867 2_2:-10079095 Msa0236990:CDS
TGCAACTGAATTACTAATCA+TGG 0.474013 2_2:-10079219 Msa0236990:CDS
AAAACTCGAGATTCGCCTTC+AGG 0.477822 2_2:-10079437 Msa0236990:CDS
AAGGCTAAGTTTAAGGGCAC+TGG 0.487685 2_2:-10078933 Msa0236990:CDS
GTCTTTGCAATTATAGAAGT+AGG 0.493739 2_2:+10078990 None:intergenic
GTTTGCTACACAGAAATCAT+TGG 0.495061 2_2:+10079466 None:intergenic
CATTGGCAGAAGATGAAACA+TGG 0.504978 2_2:+10079483 None:intergenic
GAATCAAGAAACTGAAAGCT+AGG 0.508983 2_2:+10079035 None:intergenic
TTCACCGTTGGTGATTTGCA+TGG 0.518005 2_2:+10079410 None:intergenic
TAAGTTTAAGGGCACTGGCT+AGG 0.526606 2_2:-10078928 None:intergenic
GTTCAAGGTAGAGTTACAGC+TGG 0.531322 2_2:-10079197 Msa0236990:CDS
ACTGAATTACCAAGTCTCAA+TGG 0.535559 2_2:-10079311 Msa0236990:CDS
GAATTACTAATCATGGTTCA+AGG 0.537186 2_2:-10079212 Msa0236990:CDS
GGGAGGTGTATGATGATATG+AGG 0.543192 2_2:+10079504 None:intergenic
GCATCAGGATGAGTATGCAT+TGG 0.555789 2_2:+10079239 None:intergenic
ATATCAAGTCCATTGAGACT+TGG 0.613542 2_2:+10079302 None:intergenic
AACACGAAATCATTCACCGT+TGG 0.627838 2_2:+10079398 None:intergenic
ATTGGCAGAAGATGAAACAT+GGG 0.653550 2_2:+10079484 None:intergenic
GGCAGAAGATGAAACATGGG+AGG 0.675496 2_2:+10079487 None:intergenic
TCTGCAGCAAGAGTAGACAT+TGG 0.676542 2_2:-10079278 Msa0236990:CDS
CTATCCATGCAAATCACCAA+CGG 0.680168 2_2:-10079414 Msa0236990:CDS
GATTTGCATGGATAGCCTGA+AGG 0.705863 2_2:+10079422 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GATATTATGGTTTTTCCAAA+AGG - chr2_2:10079343-10079362 Msa0236990:CDS 25.0%
! TTTCTTGATTCTGTATTGTT+TGG - chr2_2:10079448-10079467 Msa0236990:CDS 25.0%
TAATTCAGTAACTGATGCTT+TGG + chr2_2:10079149-10079168 None:intergenic 30.0%
ATTAGTAATTCAGTTGCATC+AGG + chr2_2:10079250-10079269 None:intergenic 30.0%
TGCAACTGAATTACTAATCA+TGG - chr2_2:10079252-10079271 Msa0236990:CDS 30.0%
GAATTACTAATCATGGTTCA+AGG - chr2_2:10079259-10079278 Msa0236990:CDS 30.0%
CTTTGACATAAACTTCTGTA+AGG + chr2_2:10079309-10079328 None:intergenic 30.0%
TCTTAAGCCTACTGATATTA+TGG - chr2_2:10079330-10079349 Msa0236990:CDS 30.0%
GGAAAAACCATAATATCAGT+AGG + chr2_2:10079340-10079359 None:intergenic 30.0%
! ACAAAATGCAATAGTCCTTT+TGG + chr2_2:10079361-10079380 None:intergenic 30.0%
! TTGCATTTTGTAGTTAACTC+TGG - chr2_2:10079370-10079389 Msa0236990:CDS 30.0%
GTCTTTGCAATTATAGAAGT+AGG + chr2_2:10079484-10079503 None:intergenic 30.0%
AAGCTTAAGGCTAAGTTTAA+GGG - chr2_2:10079532-10079551 Msa0236990:CDS 30.0%
TGATATGAGGAGAGCAAAAA+AGG + chr2_2:10078957-10078976 None:intergenic 35.0%
ATTGGCAGAAGATGAAACAT+GGG + chr2_2:10078990-10079009 None:intergenic 35.0%
! GTTTGCTACACAGAAATCAT+TGG + chr2_2:10079008-10079027 None:intergenic 35.0%
GTGAATGATTTCGTGTTATC+TGG - chr2_2:10079079-10079098 Msa0236990:CDS 35.0%
!! AAAGCTTTGTTTAAGCTTCC+AGG + chr2_2:10079112-10079131 None:intergenic 35.0%
ACTGAATTACCAAGTCTCAA+TGG - chr2_2:10079160-10079179 Msa0236990:CDS 35.0%
ATATCAAGTCCATTGAGACT+TGG + chr2_2:10079172-10079191 None:intergenic 35.0%
GAATCAAGAAACTGAAAGCT+AGG + chr2_2:10079439-10079458 None:intergenic 35.0%
TTCACAGATCAAGAAGCTTA+AGG - chr2_2:10079519-10079538 Msa0236990:CDS 35.0%
GAAGCTTAAGGCTAAGTTTA+AGG - chr2_2:10079531-10079550 Msa0236990:CDS 35.0%
CATTGGCAGAAGATGAAACA+TGG + chr2_2:10078991-10079010 None:intergenic 40.0%
CTATCCATGCAAATCACCAA+CGG - chr2_2:10079057-10079076 Msa0236990:CDS 40.0%
AACACGAAATCATTCACCGT+TGG + chr2_2:10079076-10079095 None:intergenic 40.0%
! GATTTCGTGTTATCTGGCTT+TGG - chr2_2:10079085-10079104 Msa0236990:CDS 40.0%
AGAGTAGACATTGGTCCAAA+TGG - chr2_2:10079202-10079221 Msa0236990:CDS 40.0%
TGCATTGGAACAGATCCATT+TGG + chr2_2:10079220-10079239 None:intergenic 40.0%
TTTGTAGTTAACTCTGGTGC+TGG - chr2_2:10079376-10079395 Msa0236990:CDS 40.0%
! GGGAGGTGTATGATGATATG+AGG + chr2_2:10078970-10078989 None:intergenic 45.0%
!! GCGAATCTCGAGTTTTGTGT+AGG + chr2_2:10079030-10079049 None:intergenic 45.0%
AAAACTCGAGATTCGCCTTC+AGG - chr2_2:10079034-10079053 Msa0236990:CDS 45.0%
GATTTGCATGGATAGCCTGA+AGG + chr2_2:10079052-10079071 None:intergenic 45.0%
!! TTCACCGTTGGTGATTTGCA+TGG + chr2_2:10079064-10079083 None:intergenic 45.0%
TCTGCAGCAAGAGTAGACAT+TGG - chr2_2:10079193-10079212 Msa0236990:CDS 45.0%
GCATCAGGATGAGTATGCAT+TGG + chr2_2:10079235-10079254 None:intergenic 45.0%
GTTCAAGGTAGAGTTACAGC+TGG - chr2_2:10079274-10079293 Msa0236990:CDS 45.0%
! AAGGCTAAGTTTAAGGGCAC+TGG - chr2_2:10079538-10079557 Msa0236990:CDS 45.0%
GGCAGAAGATGAAACATGGG+AGG + chr2_2:10078987-10079006 None:intergenic 50.0%
!! GTGTTATCTGGCTTTGGACC+TGG - chr2_2:10079091-10079110 Msa0236990:CDS 50.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 10078929 10079564 10078929 ID=Msa0236990;Name=Msa0236990
chr2_2 mRNA 10078929 10079564 10078929 ID=Msa0236990-mRNA-1;Parent=Msa0236990;Name=Msa0236990-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|211
chr2_2 exon 10078929 10079564 10078929 ID=Msa0236990-mRNA-1:exon:4328;Parent=Msa0236990-mRNA-1
chr2_2 CDS 10078929 10079564 10078929 ID=Msa0236990-mRNA-1:cds;Parent=Msa0236990-mRNA-1
Gene Sequence

>Msa0236990

ATGAAAATGAATCACATTATTGTTTCCTTTTTTGCTCTCCTCATATCATCATACACCTCCCATGTTTCATCTTCTGCCAATGATTTCTGTGTAGCAAACCTACACAAAACTCGAGATTCGCCTTCAGGCTATCCATGCAAATCACCAACGGTGAATGATTTCGTGTTATCTGGCTTTGGACCTGGAAGCTTAAACAAAGCTTTTAATGTTAAATTAGCCAAAGCATCAGTTACTGAATTACCAAGTCTCAATGGACTTGATATCTCTGCAGCAAGAGTAGACATTGGTCCAAATGGATCTGTTCCAATGCATACTCATCCTGATGCAACTGAATTACTAATCATGGTTCAAGGTAGAGTTACAGCTGGATTTATCAACCTTACAGAAGTTTATGTCAAAGATCTTAAGCCTACTGATATTATGGTTTTTCCAAAAGGACTATTGCATTTTGTAGTTAACTCTGGTGCTGGAGAAGCTATTGCTTTTGCTACTTATAGTAGCTCAAAGCCTAGCTTTCAGTTTCTTGATTCTGTATTGTTTGGAAACAAGTTACCTACTTCTATAATTGCAAAGACTACTCTTCTTGATGTTTCACAGATCAAGAAGCTTAAGGCTAAGTTTAAGGGCACTGGCTAG

Protein sequence

>Msa0236990

MKMNHIIVSFFALLISSYTSHVSSSANDFCVANLHKTRDSPSGYPCKSPTVNDFVLSGFGPGSLNKAFNVKLAKASVTELPSLNGLDISAARVDIGPNGSVPMHTHPDATELLIMVQGRVTAGFINLTEVYVKDLKPTDIMVFPKGLLHFVVNSGAGEAIAFATYSSSKPSFQFLDSVLFGNKLPTSIIAKTTLLDVSQIKKLKAKFKGTG*