AlfalfaGEDB | Msa1444440

M. sativa cultivar zhongmu-4 / Msa1444440

KEGG: -
GO: GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0048046 GO:0071944
PFAM: PF00190.25

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1444440	sp\|Q9ZRA4\|AB19A_PRUPE	47.514	181	84	5	6	181	5	179	8.61e-48	157

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1444440	G7IPZ2	96.685	181	5	1	1	180	1	181	1.18e-121	352

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0006110	Msa1444440	0.802856	4.598952e-49	-8.615850e-47
Msa0020420	Msa1444440	0.803397	3.554909e-49	-8.615850e-47
Msa0063530	Msa1444440	0.808731	2.681070e-50	-8.615850e-47
Msa0075880	Msa1444440	0.813572	2.391958e-51	-8.615850e-47
Msa0078900	Msa1444440	0.816863	4.441065e-52	-8.615850e-47
Msa0084380	Msa1444440	0.814233	1.710035e-51	-8.615850e-47
Msa0114060	Msa1444440	0.811134	8.148973e-51	-8.615850e-47
Msa1366810	Msa1444440	0.810267	1.255176e-50	-8.615850e-47
Msa1373030	Msa1444440	0.804784	1.829331e-49	-8.615850e-47
Msa1374470	Msa1444440	0.802766	4.800001e-49	-8.615850e-47
Msa1403340	Msa1444440	0.813468	2.520632e-51	-8.615850e-47
Msa1410100	Msa1444440	0.801168	1.022743e-48	-8.615850e-47
Msa1444440	Msa1449340	-0.804001	2.663123e-49	-8.615850e-47
Msa1444440	Msa1458750	0.800535	1.376898e-48	-8.615850e-47
Msa1444440	Msa1466540	0.824960	6.079936e-54	-8.615850e-47
Msa0613440	Msa1444440	0.805524	1.280508e-49	-8.615850e-47
Msa0617640	Msa1444440	0.808685	2.743001e-50	-8.615850e-47
Msa0645300	Msa1444440	0.815260	1.012925e-51	-8.615850e-47
Msa0663390	Msa1444440	0.802932	4.436810e-49	-8.615850e-47
Msa0665170	Msa1444440	0.809279	2.046460e-50	-8.615850e-47
Msa0688270	Msa1444440	0.804525	2.072246e-49	-8.615850e-47
Msa0707250	Msa1444440	0.814834	1.259001e-51	-8.615850e-47
Msa0731940	Msa1444440	0.849077	4.076660e-60	-8.615850e-47
Msa0748970	Msa1444440	0.806247	9.023339e-50	-8.615850e-47
Msa0755180	Msa1444440	0.806669	7.353038e-50	-8.615850e-47
Msa0762970	Msa1444440	0.803787	2.950859e-49	-8.615850e-47
Msa0766630	Msa1444440	0.806743	7.091258e-50	-8.615850e-47
Msa0810220	Msa1444440	0.826973	2.021914e-54	-8.615850e-47
Msa0820850	Msa1444440	0.833955	3.961522e-56	-8.615850e-47
Msa0832340	Msa1444440	0.813297	2.748329e-51	-8.615850e-47
Msa0833290	Msa1444440	0.804845	1.776705e-49	-8.615850e-47
Msa0135660	Msa1444440	0.804686	1.917637e-49	-8.615850e-47
Msa0143090	Msa1444440	0.890563	8.802493e-74	-8.615850e-47
Msa0171540	Msa1444440	0.810937	8.989082e-51	-8.615850e-47
Msa0180500	Msa1444440	0.820804	5.651942e-53	-8.615850e-47
Msa0188460	Msa1444440	0.809484	1.849409e-50	-8.615850e-47
Msa0198910	Msa1444440	0.800482	1.411484e-48	-8.615850e-47
Msa0233080	Msa1444440	0.811229	7.770786e-51	-8.615850e-47
Msa0236990	Msa1444440	0.891799	2.854358e-74	-8.615850e-47
Msa1276890	Msa1444440	0.801126	1.043223e-48	-8.615850e-47
Msa1277660	Msa1444440	0.812898	3.361826e-51	-8.615850e-47
Msa1282580	Msa1444440	-0.808052	3.742471e-50	-8.615850e-47
Msa1284680	Msa1444440	0.815384	9.503075e-52	-8.615850e-47
Msa1306920	Msa1444440	0.809830	1.558947e-50	-8.615850e-47
Msa0381610	Msa1444440	-0.806612	7.560059e-50	-8.615850e-47
Msa0390070	Msa1444440	0.800215	1.600103e-48	-8.615850e-47
Msa0397750	Msa1444440	0.801208	1.003440e-48	-8.615850e-47
Msa0434180	Msa1444440	0.807441	5.048216e-50	-8.615850e-47
Msa0998690	Msa1444440	0.801399	9.171061e-49	-8.615850e-47
Msa1014540	Msa1444440	0.822497	2.295199e-53	-8.615850e-47
Msa1017350	Msa1444440	0.804284	2.326066e-49	-8.615850e-47
Msa1023950	Msa1444440	0.824004	1.020583e-53	-8.615850e-47
Msa1035480	Msa1444440	0.820833	5.565972e-53	-8.615850e-47
Msa1065570	Msa1444440	0.804799	1.816380e-49	-8.615850e-47
Msa1075540	Msa1444440	0.810447	1.147467e-50	-8.615850e-47
Msa1075650	Msa1444440	0.801090	1.060962e-48	-8.615850e-47
Msa1104090	Msa1444440	0.802951	4.396871e-49	-8.615850e-47
Msa0251640	Msa1444440	0.818971	1.483185e-52	-8.615850e-47
Msa0267370	Msa1444440	0.810600	1.063592e-50	-8.615850e-47
Msa0280090	Msa1444440	0.804913	1.719382e-49	-8.615850e-47
Msa0289330	Msa1444440	0.804447	2.150410e-49	-8.615850e-47
Msa0290870	Msa1444440	0.803298	3.726244e-49	-8.615850e-47
Msa0305190	Msa1444440	0.810431	1.156839e-50	-8.615850e-47
Msa0342750	Msa1444440	0.813433	2.566423e-51	-8.615850e-47
Msa1109440	Msa1444440	0.804550	2.046721e-49	-8.615850e-47
Msa1109680	Msa1444440	0.829374	5.336141e-55	-8.615850e-47
Msa1111870	Msa1444440	0.808152	3.564596e-50	-8.615850e-47
Msa1113170	Msa1444440	0.827452	1.552686e-54	-8.615850e-47
Msa1115100	Msa1444440	0.810493	1.121346e-50	-8.615850e-47
Msa1117150	Msa1444440	0.800756	1.241479e-48	-8.615850e-47
Msa1119480	Msa1444440	0.813716	2.223910e-51	-8.615850e-47
Msa1121480	Msa1444440	0.830436	2.939752e-55	-8.615850e-47
Msa1121490	Msa1444440	0.813752	2.183060e-51	-8.615850e-47
Msa1137570	Msa1444440	0.808472	3.045621e-50	-8.615850e-47
Msa1146120	Msa1444440	0.806028	1.003378e-49	-8.615850e-47
Msa1154280	Msa1444440	0.814866	1.238737e-51	-8.615850e-47
Msa1156020	Msa1444440	0.803177	3.947284e-49	-8.615850e-47
Msa1162780	Msa1444440	0.822519	2.268911e-53	-8.615850e-47
Msa1181250	Msa1444440	0.818396	2.003795e-52	-8.615850e-47
Msa1181330	Msa1444440	0.818895	1.544097e-52	-8.615850e-47
Msa1181910	Msa1444440	0.802587	5.226785e-49	-8.615850e-47
Msa1194160	Msa1444440	0.814459	1.524266e-51	-8.615850e-47
Msa1195190	Msa1444440	0.801200	1.007257e-48	-8.615850e-47
Msa1199300	Msa1444440	0.802750	4.838373e-49	-8.615850e-47
Msa0490140	Msa1444440	0.803521	3.349986e-49	-8.615850e-47
Msa0503430	Msa1444440	0.806960	6.383118e-50	-8.615850e-47
Msa0529890	Msa1444440	0.800776	1.229590e-48	-8.615850e-47
Msa0530400	Msa1444440	0.818376	2.024920e-52	-8.615850e-47
Msa0545700	Msa1444440	0.822553	2.227420e-53	-8.615850e-47
Msa0585560	Msa1444440	0.804353	2.250107e-49	-8.615850e-47
Msa0592440	Msa1444440	0.819775	9.730895e-53	-8.615850e-47
Msa0598780	Msa1444440	0.812092	5.045607e-51	-8.615850e-47
Msa0844490	Msa1444440	0.829271	5.652461e-55	-8.615850e-47
Msa0848960	Msa1444440	0.801121	1.045328e-48	-8.615850e-47
Msa0858130	Msa1444440	0.810072	1.382733e-50	-8.615850e-47
Msa0861130	Msa1444440	0.832946	7.069404e-56	-8.615850e-47
Msa0897330	Msa1444440	0.833533	5.048577e-56	-8.615850e-47
Msa0899430	Msa1444440	0.810719	1.002125e-50	-8.615850e-47
Msa0899450	Msa1444440	0.812288	4.572250e-51	-8.615850e-47
Msa0927380	Msa1444440	0.817159	3.810673e-52	-8.615850e-47
Msa0928130	Msa1444440	0.804205	2.415792e-49	-8.615850e-47
Msa0958550	Msa1444440	0.812443	4.228239e-51	-8.615850e-47
Msa0964900	Msa1444440	0.802790	4.745915e-49	-8.615850e-47
Msa0973100	Msa1444440	0.801314	9.544045e-49	-8.615850e-47
Msa0974070	Msa1444440	0.828557	8.413922e-55	-8.615850e-47
Msa0974080	Msa1444440	0.835749	1.399772e-56	-8.615850e-47
Msa0976670	Msa1444440	0.819424	1.169784e-52	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1444440	MtrunA17_Chr2g0286171	96.685	181	5	1	1	180	1	181	2.28e-125	352
Msa1444440	MtrunA17_Chr3g0115141	83.060	183	30	1	1	183	1	182	1.04e-97	281
Msa1444440	MtrunA17_Chr4g0003361	61.878	181	59	5	3	180	1	174	7.61e-67	203
Msa1444440	MtrunA17_Chr8g0343934	56.284	183	72	4	1	180	1	178	2.66e-63	194
Msa1444440	MtrunA17_Chr8g0343954	54.645	183	75	3	1	180	1	178	1.66e-62	192
Msa1444440	MtrunA17_Chr8g0344021	54.348	184	77	4	1	180	1	181	3.27e-59	184
Msa1444440	MtrunA17_Chr8g0344031	55.191	183	76	4	1	180	1	180	1.03e-58	182
Msa1444440	MtrunA17_Chr8g0344041	54.891	184	73	5	1	180	5	182	1.29e-58	182
Msa1444440	MtrunA17_Chr8g0343961	53.552	183	77	4	1	180	1	178	1.46e-58	182
Msa1444440	MtrunA17_Chr2g0301611	52.459	183	79	4	1	180	1	178	7.31e-57	178
Msa1444440	MtrunA17_Chr2g0302541	55.280	161	68	2	23	180	19	178	2.20e-54	171
Msa1444440	MtrunA17_Chr2g0313961	49.727	183	84	4	1	180	1	178	4.41e-54	171
Msa1444440	MtrunA17_Chr2g0297231	49.727	183	84	4	1	180	1	178	1.89e-53	169
Msa1444440	MtrunA17_Chr2g0302171	50.270	185	80	4	1	180	1	178	2.84e-53	169
Msa1444440	MtrunA17_Chr7g0217811	56.688	157	64	2	27	180	23	178	3.16e-53	169
Msa1444440	MtrunA17_Chr8g0344051	49.189	185	85	5	1	180	1	181	9.84e-53	167
Msa1444440	MtrunA17_Chr3g0084531	50.276	181	84	3	3	180	1	178	2.15e-52	166
Msa1444440	MtrunA17_Chr2g0297241	48.649	185	83	4	1	180	1	178	3.58e-52	166
Msa1444440	MtrunA17_Chr8g0343911	46.023	176	89	4	14	184	7	181	2.14e-51	164
Msa1444440	MtrunA17_Chr4g0012651	54.777	157	67	2	27	180	23	178	2.40e-51	164
Msa1444440	MtrunA17_Chr5g0411961	49.180	183	85	4	1	180	1	178	1.24e-50	162
Msa1444440	MtrunA17_Chr3g0084521	49.180	183	84	4	1	180	1	177	1.26e-50	162
Msa1444440	MtrunA17_Chr3g0084511	48.634	183	85	4	1	180	1	177	6.49e-50	160
Msa1444440	MtrunA17_Chr8g0343921	45.143	175	90	5	11	180	3	176	6.20e-44	145
Msa1444440	MtrunA17_Chr2g0300561	41.799	189	99	5	1	184	1	183	5.59e-43	142
Msa1444440	MtrunA17_Chr2g0300581	41.270	189	100	5	1	184	1	183	6.23e-43	142
Msa1444440	MtrunA17_Chr2g0300591	41.270	189	100	5	1	184	1	183	6.23e-43	142
Msa1444440	MtrunA17_Chr3g0084551	43.243	185	91	5	3	183	1	175	1.68e-36	126
Msa1444440	MtrunA17_Chr1g0189841	39.665	179	100	3	1	171	1	179	8.78e-33	117
Msa1444440	MtrunA17_Chr4g0007041	34.872	195	116	5	1	186	1	193	1.87e-26	100
Msa1444440	MtrunA17_Chr4g0007051	34.872	195	116	5	1	186	1	193	4.20e-26	99.8
Msa1444440	MtrunA17_Chr4g0007031	34.872	195	116	5	1	186	1	193	4.48e-26	99.8
Msa1444440	MtrunA17_Chr5g0419971	34.872	195	116	5	1	186	1	193	4.48e-26	99.8
Msa1444440	MtrunA17_Chr5g0419981	34.872	195	116	5	1	186	1	193	4.48e-26	99.8
Msa1444440	MtrunA17_Chr6g0449761	37.079	178	102	5	1	170	1	176	1.55e-25	98.6
Msa1444440	MtrunA17_Chr2g0285871	34.359	195	117	5	1	186	1	193	1.71e-25	98.2
Msa1444440	MtrunA17_Chr6g0449771	37.079	178	102	5	1	170	1	176	2.81e-25	97.4
Msa1444440	MtrunA17_Chr6g0449791	35.955	178	105	4	1	170	1	177	6.66e-25	96.3
Msa1444440	MtrunA17_Chr8g0335851	37.594	133	79	2	42	170	47	179	7.40e-25	96.3
Msa1444440	MtrunA17_Chr6g0449731	37.079	178	102	5	1	170	1	176	7.56e-25	96.3
Msa1444440	MtrunA17_Chr1g0193421	36.478	159	95	4	27	180	29	186	7.71e-25	96.3
Msa1444440	MtrunA17_Chr6g0449751	37.853	177	102	4	1	170	1	176	8.95e-25	96.3
Msa1444440	MtrunA17_Chr1g0178711	34.146	164	98	4	27	180	31	194	3.56e-23	92.0
Msa1444440	MtrunA17_Chr3g0084501	46.429	112	48	4	1	107	1	105	4.73e-23	88.6
Msa1444440	MtrunA17_Chr1g0193671	31.892	185	120	4	4	183	5	188	1.60e-22	90.1
Msa1444440	MtrunA17_Chr2g0292831	36.364	165	94	4	23	180	23	183	2.43e-22	89.7
Msa1444440	MtrunA17_Chr3g0130241	33.158	190	111	5	1	177	63	249	6.38e-22	90.1
Msa1444440	MtrunA17_Chr2g0292901	34.545	165	97	4	23	180	24	184	1.21e-21	87.8
Msa1444440	MtrunA17_Chr5g0414971	32.919	161	101	5	28	182	33	192	2.59e-20	84.3
Msa1444440	MtrunA17_Chr7g0271211	36.735	147	87	4	27	168	25	170	3.41e-20	84.0
Msa1444440	MtrunA17_Chr1g0156131	33.333	165	96	4	23	180	23	180	7.01e-20	83.2
Msa1444440	MtrunA17_Chr1g0210761	33.333	159	93	7	28	180	29	180	8.82e-20	82.8
Msa1444440	MtrunA17_Chr4g0026201	40.000	115	67	2	71	183	67	181	3.66e-19	81.3
Msa1444440	MtrunA17_Chr2g0321591	39.286	112	66	2	74	183	69	180	6.00e-19	80.5
Msa1444440	MtrunA17_Chr4g0026191	39.130	115	68	2	71	183	67	181	1.21e-18	79.7
Msa1444440	MtrunA17_Chr2g0321581	38.393	112	67	2	74	183	69	180	2.52e-18	79.0
Msa1444440	MtrunA17_Chr6g0449781	35.374	147	87	4	28	167	28	173	2.71e-17	76.3
Msa1444440	MtrunA17_Chr2g0321601	37.069	116	69	4	71	183	65	179	2.92e-17	76.3
Msa1444440	MtrunA17_Chr2g0327651	38.614	101	60	1	47	145	7	107	5.78e-17	73.2
Msa1444440	MtrunA17_Chr2g0292861	34.266	143	86	4	46	180	7	149	6.13e-17	74.7
Msa1444440	MtrunA17_Chr2g0292911	37.607	117	66	3	46	155	7	123	4.52e-16	71.2
Msa1444440	MtrunA17_Chr2g0292841	33.566	143	87	4	46	180	7	149	2.20e-15	70.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1444440	AT1G72610.1	46.988	166	82	4	23	183	16	180	4.02e-48	156
Msa1444440	AT5G20630.1	43.716	183	95	5	3	180	1	180	2.00e-41	139
Msa1444440	AT5G39160.3	35.754	179	101	5	1	169	1	175	1.54e-28	106
Msa1444440	AT1G02335.1	33.149	181	113	5	6	180	8	186	3.62e-28	105
Msa1444440	AT5G39160.1	35.196	179	106	4	1	169	1	179	1.23e-27	103
Msa1444440	AT5G39190.1	35.196	179	106	4	1	169	1	179	1.43e-27	103
Msa1444440	AT5G39110.1	37.791	172	98	4	6	168	8	179	1.55e-27	103
Msa1444440	AT5G39130.1	34.637	179	107	4	1	169	1	179	3.61e-27	102
Msa1444440	AT5G39120.1	36.264	182	96	6	1	168	3	178	5.17e-27	102
Msa1444440	AT3G04190.1	33.918	171	105	3	24	186	28	198	2.40e-26	100
Msa1444440	AT4G14630.1	37.209	172	97	5	8	169	12	182	9.57e-26	99.0
Msa1444440	AT1G09560.1	36.628	172	98	5	22	183	19	189	1.44e-25	98.6
Msa1444440	AT5G39180.1	33.503	197	111	6	1	183	3	193	1.59e-25	98.6
Msa1444440	AT5G39150.1	35.714	182	97	6	1	168	3	178	2.14e-25	98.2
Msa1444440	AT3G04200.1	35.795	176	95	4	8	170	13	183	3.26e-25	97.8
Msa1444440	AT3G05950.1	36.257	171	97	4	12	170	12	182	5.71e-25	97.4
Msa1444440	AT5G38960.1	36.747	166	96	4	24	180	28	193	8.81e-25	96.7
Msa1444440	AT3G04150.1	31.548	168	107	3	27	186	31	198	1.48e-24	96.3
Msa1444440	AT5G61750.1	38.060	134	78	3	42	170	40	173	1.62e-24	95.5
Msa1444440	AT3G05930.1	33.514	185	113	6	3	180	5	186	1.03e-23	93.6
Msa1444440	AT5G39160.2	32.000	175	95	3	1	169	1	157	2.03e-23	92.4
Msa1444440	AT5G39190.2	32.000	175	95	3	1	169	1	157	2.23e-23	92.4
Msa1444440	AT3G04180.1	32.584	178	98	4	24	186	28	198	2.64e-23	92.8
Msa1444440	AT1G18970.1	36.416	173	106	4	11	180	18	189	2.88e-23	92.4
Msa1444440	AT1G18980.1	37.342	158	93	5	27	180	34	189	7.45e-23	91.7
Msa1444440	AT5G26700.1	32.044	181	114	5	5	180	7	183	9.22e-23	91.3
Msa1444440	AT5G38940.1	38.065	155	88	4	24	170	28	182	1.08e-22	91.3
Msa1444440	AT5G38940.2	38.065	155	88	4	24	170	52	206	1.19e-22	91.7
Msa1444440	AT5G38910.1	36.628	172	101	4	7	170	9	180	2.16e-22	90.5
Msa1444440	AT3G62020.1	33.125	160	100	5	27	180	28	186	6.16e-22	89.0
Msa1444440	AT3G04150.2	29.282	181	107	4	27	186	31	211	8.77e-22	89.4
Msa1444440	AT3G04170.1	30.978	184	116	4	8	183	12	192	1.07e-21	88.6
Msa1444440	AT3G62020.2	33.793	145	90	4	42	180	13	157	3.96e-21	86.3
Msa1444440	AT3G10080.1	34.545	165	94	4	27	180	31	192	3.28e-20	84.7
Msa1444440	AT1G74820.1	34.091	176	105	5	6	171	13	187	4.45e-20	84.3
Msa1444440	AT3G10080.2	34.545	165	94	4	27	180	102	263	4.96e-20	85.5
Msa1444440	AT1G10460.1	33.544	158	96	6	27	175	24	181	5.90e-20	84.0
Msa1444440	AT5G38930.1	36.129	155	91	4	24	170	28	182	8.96e-20	83.6
Msa1444440	AT5G38910.2	31.361	169	77	4	7	170	9	143	6.78e-11	58.9

Find 31 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCTTAAGCCTACTGATATTA+TGG	0.242052	tig0030729:+1766	Msa1444440:CDS
GTGAATGATTTCGTGTTATC+TGG	0.259765	tig0030729:+1515	Msa1444440:CDS
ACAAAATGCAATAGTCCTTT+TGG	0.270307	tig0030729:-1794	None:intergenic
AAAGCTTTGTTTAAGCTTCC+AGG	0.294960	tig0030729:-1545	None:intergenic
GATTTCGTGTTATCTGGCTT+TGG	0.389146	tig0030729:+1521	Msa1444440:CDS
TAATTCAGTAACTGATGCTT+TGG	0.397550	tig0030729:-1582	None:intergenic
GTGTTATCTGGCTTTGGACC+TGG	0.438197	tig0030729:+1527	Msa1444440:CDS
TGCATTGGAACAGATCCATT+TGG	0.439588	tig0030729:-1653	None:intergenic
ATTAGTAATTCAGTTGCATC+AGG	0.439691	tig0030729:-1683	None:intergenic
AGAGTAGACATTGGTCCAAA+TGG	0.456817	tig0030729:+1638	Msa1444440:CDS
CTTTGACATAAACTTCTGTA+AGG	0.459348	tig0030729:-1742	None:intergenic
TTTGTAGTTAACTCTGGTGC+TGG	0.460867	tig0030729:+1812	Msa1444440:CDS
TGCAACTGAATTACTAATCA+TGG	0.474013	tig0030729:+1688	Msa1444440:CDS
AAAACTCGAGATTCGCCTTC+AGG	0.477822	tig0030729:+1470	Msa1444440:CDS
GTCTTTGCAATTATAGAAGT+AGG	0.493739	tig0030729:-1916	None:intergenic
GTTTGCTACACAGAAATCAT+TGG	0.495061	tig0030729:-1441	None:intergenic
CATTGGCAGAAGATGAAACA+TGG	0.504978	tig0030729:-1424	None:intergenic
GAATCAAGAAACTGAAAGCT+AGG	0.508983	tig0030729:-1872	None:intergenic
TTCACCGTTGGTGATTTGCA+TGG	0.518005	tig0030729:-1497	None:intergenic
GTTCAAGGTAGAGTTACAGC+TGG	0.531322	tig0030729:+1710	Msa1444440:CDS
ACTGAATTACCAAGTCTCAA+TGG	0.535559	tig0030729:+1596	Msa1444440:CDS
GAATTACTAATCATGGTTCA+AGG	0.537186	tig0030729:+1695	Msa1444440:CDS
GGGAGGTGTATGATGATATG+AGG	0.543192	tig0030729:-1403	None:intergenic
GCATCAGGATGAGTATGCAT+TGG	0.555789	tig0030729:-1668	None:intergenic
ATATCAAGTCCATTGAGACT+TGG	0.613542	tig0030729:-1605	None:intergenic
AACACGAAATCATTCACCGT+TGG	0.627838	tig0030729:-1509	None:intergenic
ATTGGCAGAAGATGAAACAT+GGG	0.653550	tig0030729:-1423	None:intergenic
GGCAGAAGATGAAACATGGG+AGG	0.675496	tig0030729:-1420	None:intergenic
TCTGCAGCAAGAGTAGACAT+TGG	0.676542	tig0030729:+1629	Msa1444440:CDS
CTATCCATGCAAATCACCAA+CGG	0.680168	tig0030729:+1493	Msa1444440:CDS
GATTTGCATGGATAGCCTGA+AGG	0.705863	tig0030729:-1485	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	GATATTATGGTTTTTCCAAA+AGG	+	tig0030729:1779-1798	Msa1444440:CDS	25.0%
	TAATTCAGTAACTGATGCTT+TGG	-	tig0030729:1585-1604	None:intergenic	30.0%
	ATTAGTAATTCAGTTGCATC+AGG	-	tig0030729:1686-1705	None:intergenic	30.0%
	TGCAACTGAATTACTAATCA+TGG	+	tig0030729:1688-1707	Msa1444440:CDS	30.0%
	GAATTACTAATCATGGTTCA+AGG	+	tig0030729:1695-1714	Msa1444440:CDS	30.0%
	CTTTGACATAAACTTCTGTA+AGG	-	tig0030729:1745-1764	None:intergenic	30.0%
	TCTTAAGCCTACTGATATTA+TGG	+	tig0030729:1766-1785	Msa1444440:CDS	30.0%
	GGAAAAACCATAATATCAGT+AGG	-	tig0030729:1776-1795	None:intergenic	30.0%
!	ACAAAATGCAATAGTCCTTT+TGG	-	tig0030729:1797-1816	None:intergenic	30.0%
!	TTGCATTTTGTAGTTAACTC+TGG	+	tig0030729:1806-1825	Msa1444440:CDS	30.0%
	TGATATGAGGAGAGCAAAAA+AGG	-	tig0030729:1393-1412	None:intergenic	35.0%
	ATTGGCAGAAGATGAAACAT+GGG	-	tig0030729:1426-1445	None:intergenic	35.0%
!	GTTTGCTACACAGAAATCAT+TGG	-	tig0030729:1444-1463	None:intergenic	35.0%
	GTGAATGATTTCGTGTTATC+TGG	+	tig0030729:1515-1534	Msa1444440:CDS	35.0%
!!	AAAGCTTTGTTTAAGCTTCC+AGG	-	tig0030729:1548-1567	None:intergenic	35.0%
	ACTGAATTACCAAGTCTCAA+TGG	+	tig0030729:1596-1615	Msa1444440:CDS	35.0%
	ATATCAAGTCCATTGAGACT+TGG	-	tig0030729:1608-1627	None:intergenic	35.0%
	GAATCAAGAAACTGAAAGCT+AGG	-	tig0030729:1875-1894	None:intergenic	35.0%
	CATTGGCAGAAGATGAAACA+TGG	-	tig0030729:1427-1446	None:intergenic	40.0%
	CTATCCATGCAAATCACCAA+CGG	+	tig0030729:1493-1512	Msa1444440:CDS	40.0%
	AACACGAAATCATTCACCGT+TGG	-	tig0030729:1512-1531	None:intergenic	40.0%
!	GATTTCGTGTTATCTGGCTT+TGG	+	tig0030729:1521-1540	Msa1444440:CDS	40.0%
	AGAGTAGACATTGGTCCAAA+TGG	+	tig0030729:1638-1657	Msa1444440:CDS	40.0%
	TGCATTGGAACAGATCCATT+TGG	-	tig0030729:1656-1675	None:intergenic	40.0%
	TTTGTAGTTAACTCTGGTGC+TGG	+	tig0030729:1812-1831	Msa1444440:CDS	40.0%
!	GGGAGGTGTATGATGATATG+AGG	-	tig0030729:1406-1425	None:intergenic	45.0%
!!	GCGAATCTCGAGTTTTGTGT+AGG	-	tig0030729:1466-1485	None:intergenic	45.0%
	AAAACTCGAGATTCGCCTTC+AGG	+	tig0030729:1470-1489	Msa1444440:CDS	45.0%
	GATTTGCATGGATAGCCTGA+AGG	-	tig0030729:1488-1507	None:intergenic	45.0%
!!	TTCACCGTTGGTGATTTGCA+TGG	-	tig0030729:1500-1519	None:intergenic	45.0%
	TCTGCAGCAAGAGTAGACAT+TGG	+	tig0030729:1629-1648	Msa1444440:CDS	45.0%
	GCATCAGGATGAGTATGCAT+TGG	-	tig0030729:1671-1690	None:intergenic	45.0%
	GTTCAAGGTAGAGTTACAGC+TGG	+	tig0030729:1710-1729	Msa1444440:CDS	45.0%
	GGCAGAAGATGAAACATGGG+AGG	-	tig0030729:1423-1442	None:intergenic	50.0%
!!	GTGTTATCTGGCTTTGGACC+TGG	+	tig0030729:1527-1546	Msa1444440:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
tig0030729	gene	1365	1928	1365	ID=Msa1444440;Name=Msa1444440
tig0030729	mRNA	1365	1928	1365	ID=Msa1444440-mRNA-1;Parent=Msa1444440;Name=Msa1444440-mRNA-1;_AED=0.05;_eAED=0.05;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|187
tig0030729	exon	1365	1928	1365	ID=Msa1444440-mRNA-1:exon:14480;Parent=Msa1444440-mRNA-1
tig0030729	CDS	1365	1928	1365	ID=Msa1444440-mRNA-1:cds;Parent=Msa1444440-mRNA-1

Gene Sequence

>Msa1444440

ATGAAAATGAATCACATTATTGTTTCCTTTTTTGCTCTCCTCATATCATCATACACCTCCCATGTTTCATCTTCTGCCAATGATTTCTGTGTAGCAAACCTACACAAAACTCGAGATTCGCCTTCAGGCTATCCATGCAAATCACCAACGGTGAATGATTTCGTGTTATCTGGCTTTGGACCTGGAAGCTTAAACAAAGCTTTTAATGTTAAATTAGCCAAAGCATCAGTTACTGAATTACCAAGTCTCAATGGACTTGATATCTCTGCAGCAAGAGTAGACATTGGTCCAAATGGATCTGTTCCAATGCATACTCATCCTGATGCAACTGAATTACTAATCATGGTTCAAGGTAGAGTTACAGCTGGATTTATCAACCTTACAGAAGTTTATGTCAAAGATCTTAAGCCTACTGATATTATGGTTTTTCCAAAAGGACTATTGCATTTTGTAGTTAACTCTGGTGCTGGAGAAGCTATTGCTTTTGCTACTTATAGTAGCTCAAAGCCTAGCTTTCAGTTTCTTGATTCTGTATTGTTTGAAACAAGTTACCTACTTCTATAA

Protein sequence

>Msa1444440

MKMNHIIVSFFALLISSYTSHVSSSANDFCVANLHKTRDSPSGYPCKSPTVNDFVLSGFGPGSLNKAFNVKLAKASVTELPSLNGLDISAARVDIGPNGSVPMHTHPDATELLIMVQGRVTAGFINLTEVYVKDLKPTDIMVFPKGLLHFVVNSGAGEAIAFATYSSSKPSFQFLDSVLFETSYLLL*