Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0253130 | XP_007149284.1 | 75.641 | 78 | 19 | 0 | 1 | 78 | 1 | 78 | 3.29e-37 | 129 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0253130 | sp|Q9SKY1|CSTM4_ARATH | 50.000 | 78 | 32 | 4 | 1 | 78 | 1 | 71 | 1.37e-16 | 70.1 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0253130 | V7BW57 | 75.641 | 78 | 19 | 0 | 1 | 78 | 1 | 78 | 1.57e-37 | 129 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0212710 | Msa0253130 | 0.802393 | 5.729987e-49 | -8.615850e-47 |
| Msa0253130 | Msa0515590 | 0.803727 | 3.035919e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0253130 | MtrunA17_Chr2g0312051 | 91.026 | 78 | 5 | 1 | 1 | 78 | 1 | 76 | 1.32e-38 | 122 |
| Msa0253130 | MtrunA17_Chr1g0166081 | 79.747 | 79 | 15 | 1 | 1 | 78 | 1 | 79 | 9.43e-36 | 115 |
| Msa0253130 | MtrunA17_Chr5g0417951 | 78.481 | 79 | 16 | 1 | 1 | 78 | 1 | 79 | 3.52e-35 | 114 |
| Msa0253130 | MtrunA17_Chr4g0048281 | 83.750 | 80 | 11 | 1 | 1 | 78 | 1 | 80 | 6.01e-34 | 111 |
| Msa0253130 | MtrunA17_Chr2g0312011 | 57.143 | 63 | 19 | 3 | 1 | 62 | 1 | 56 | 4.26e-14 | 60.8 |
| Msa0253130 | MtrunA17_Chr2g0312031 | 87.179 | 39 | 5 | 0 | 40 | 78 | 16 | 54 | 6.23e-12 | 54.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0253130 | AT2G32190.1 | 50.000 | 78 | 32 | 4 | 1 | 78 | 1 | 71 | 1.39e-17 | 70.1 |
| Msa0253130 | AT2G32210.1 | 50.000 | 78 | 32 | 4 | 1 | 78 | 1 | 71 | 1.10e-16 | 67.8 |
| Msa0253130 | AT2G32200.1 | 43.210 | 81 | 36 | 5 | 1 | 78 | 1 | 74 | 2.30e-11 | 54.3 |
| Msa0253130 | AT2G32200.2 | 43.421 | 76 | 34 | 4 | 6 | 78 | 39 | 108 | 2.76e-11 | 55.1 |
Find 32 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTGGTGGTGGATATGATAC+TGG | 0.277131 | 2_2:-44755582 | None:intergenic |
| CATCACCCCTGCTAGTAGTT+TGG | 0.398219 | 2_2:-44755704 | None:intergenic |
| CTGGTGGTGGATATGATACT+GGG | 0.436288 | 2_2:-44755581 | None:intergenic |
| GGTGGTGGGGCAGTTACATA+TGG | 0.448724 | 2_2:-44755624 | None:intergenic |
| GAAGGATAACCCATTGGTGG+TGG | 0.456925 | 2_2:-44755639 | None:intergenic |
| TGTAACTGCCCCACCACCAA+TGG | 0.466214 | 2_2:+44755629 | Msa0253130:CDS |
| CCCCTGCTAGTAGTTTGGTC+TGG | 0.475028 | 2_2:-44755699 | None:intergenic |
| TAGCTTAAAAGCATATATCA+AGG | 0.479451 | 2_2:-44755770 | None:intergenic |
| TGGTCTGGAATCCTTTGCTG+AGG | 0.526345 | 2_2:-44755684 | None:intergenic |
| TTCCAGACCAAACTACTAGC+AGG | 0.528828 | 2_2:+44755697 | Msa0253130:CDS |
| GTAACTGCCCCACCACCAAT+GGG | 0.530036 | 2_2:+44755630 | Msa0253130:CDS |
| GGTCTGGAATCCTTTGCTGA+GGG | 0.537531 | 2_2:-44755683 | None:intergenic |
| CCTTCCAAGGATGTCTCTGA+AGG | 0.548959 | 2_2:+44755657 | Msa0253130:CDS |
| GTACCCTTCAGAGACATCCT+TGG | 0.558950 | 2_2:-44755661 | None:intergenic |
| AAGGATAACCCATTGGTGGT+GGG | 0.561789 | 2_2:-44755638 | None:intergenic |
| AGGATAACCCATTGGTGGTG+GGG | 0.564664 | 2_2:-44755637 | None:intergenic |
| GAATATGCCTCACCTGGTGG+TGG | 0.568406 | 2_2:-44755594 | None:intergenic |
| TTGGAAGGATAACCCATTGG+TGG | 0.578593 | 2_2:-44755642 | None:intergenic |
| ACCAATGGGTTATCCTTCCA+AGG | 0.585220 | 2_2:+44755644 | Msa0253130:CDS |
| CAAACTACTAGCAGGGGTGA+TGG | 0.586318 | 2_2:+44755705 | Msa0253130:CDS |
| TCCAGACCAAACTACTAGCA+GGG | 0.592593 | 2_2:+44755698 | Msa0253130:CDS |
| CCAGTATCATATCCACCACC+AGG | 0.598096 | 2_2:+44755582 | Msa0253130:CDS |
| TCCTTGGAAGGATAACCCAT+TGG | 0.604020 | 2_2:-44755645 | None:intergenic |
| ATCATATCCACCACCAGGTG+AGG | 0.605203 | 2_2:+44755587 | Msa0253130:CDS |
| CTTCCAAGGATGTCTCTGAA+GGG | 0.620825 | 2_2:+44755658 | Msa0253130:CDS |
| AGCTTAAAAGCATATATCAA+GGG | 0.622395 | 2_2:-44755769 | None:intergenic |
| GCTGAAGAATATGCCTCACC+TGG | 0.625771 | 2_2:-44755600 | None:intergenic |
| CCTTCAGAGACATCCTTGGA+AGG | 0.637821 | 2_2:-44755657 | None:intergenic |
| CTGAAGGGTACCCTCAGCAA+AGG | 0.645351 | 2_2:+44755673 | Msa0253130:CDS |
| CCAGACCAAACTACTAGCAG+GGG | 0.657589 | 2_2:+44755699 | Msa0253130:CDS |
| TGGTGGTGGATATGATACTG+GGG | 0.671764 | 2_2:-44755580 | None:intergenic |
| GAAGAATATGCCTCACCTGG+TGG | 0.723515 | 2_2:-44755597 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TGGTGGTGGATATGATACTG+GGG | - | chr2_2:44755583-44755602 | None:intergenic | 45.0% |
| !! | CTGGTGGTGGATATGATACT+GGG | - | chr2_2:44755584-44755603 | None:intergenic | 45.0% |
| ! | AAGGATAACCCATTGGTGGT+GGG | - | chr2_2:44755641-44755660 | None:intergenic | 45.0% |
| TTGGAAGGATAACCCATTGG+TGG | - | chr2_2:44755645-44755664 | None:intergenic | 45.0% | |
| !! | ACCAATGGGTTATCCTTCCA+AGG | + | chr2_2:44755644-44755663 | Msa0253130:CDS | 45.0% |
| TCCTTGGAAGGATAACCCAT+TGG | - | chr2_2:44755648-44755667 | None:intergenic | 45.0% | |
| CTTCCAAGGATGTCTCTGAA+GGG | + | chr2_2:44755658-44755677 | Msa0253130:CDS | 45.0% | |
| TTCCAGACCAAACTACTAGC+AGG | + | chr2_2:44755697-44755716 | Msa0253130:CDS | 45.0% | |
| TCCAGACCAAACTACTAGCA+GGG | + | chr2_2:44755698-44755717 | Msa0253130:CDS | 45.0% | |
| !! | AGGGGTGATGGTTTTTGGAA+GGG | + | chr2_2:44755717-44755736 | Msa0253130:CDS | 45.0% |
| !! | CCTGGTGGTGGATATGATAC+TGG | - | chr2_2:44755585-44755604 | None:intergenic | 50.0% |
| CCAGTATCATATCCACCACC+AGG | + | chr2_2:44755582-44755601 | Msa0253130:CDS | 50.0% | |
| ATCATATCCACCACCAGGTG+AGG | + | chr2_2:44755587-44755606 | Msa0253130:CDS | 50.0% | |
| GAAGAATATGCCTCACCTGG+TGG | - | chr2_2:44755600-44755619 | None:intergenic | 50.0% | |
| GCTGAAGAATATGCCTCACC+TGG | - | chr2_2:44755603-44755622 | None:intergenic | 50.0% | |
| !! | AGGATAACCCATTGGTGGTG+GGG | - | chr2_2:44755640-44755659 | None:intergenic | 50.0% |
| GAAGGATAACCCATTGGTGG+TGG | - | chr2_2:44755642-44755661 | None:intergenic | 50.0% | |
| CCTTCAGAGACATCCTTGGA+AGG | - | chr2_2:44755660-44755679 | None:intergenic | 50.0% | |
| CCTTCCAAGGATGTCTCTGA+AGG | + | chr2_2:44755657-44755676 | Msa0253130:CDS | 50.0% | |
| GTACCCTTCAGAGACATCCT+TGG | - | chr2_2:44755664-44755683 | None:intergenic | 50.0% | |
| ! | GGTCTGGAATCCTTTGCTGA+GGG | - | chr2_2:44755686-44755705 | None:intergenic | 50.0% |
| ! | TGGTCTGGAATCCTTTGCTG+AGG | - | chr2_2:44755687-44755706 | None:intergenic | 50.0% |
| CCAGACCAAACTACTAGCAG+GGG | + | chr2_2:44755699-44755718 | Msa0253130:CDS | 50.0% | |
| CATCACCCCTGCTAGTAGTT+TGG | - | chr2_2:44755707-44755726 | None:intergenic | 50.0% | |
| CAAACTACTAGCAGGGGTGA+TGG | + | chr2_2:44755705-44755724 | Msa0253130:CDS | 50.0% | |
| !! | CTAGCAGGGGTGATGGTTTT+TGG | + | chr2_2:44755712-44755731 | Msa0253130:CDS | 50.0% |
| !! | CAGGGGTGATGGTTTTTGGA+AGG | + | chr2_2:44755716-44755735 | Msa0253130:CDS | 50.0% |
| GAATATGCCTCACCTGGTGG+TGG | - | chr2_2:44755597-44755616 | None:intergenic | 55.0% | |
| !! | GGTGGTGGGGCAGTTACATA+TGG | - | chr2_2:44755627-44755646 | None:intergenic | 55.0% |
| TGTAACTGCCCCACCACCAA+TGG | + | chr2_2:44755629-44755648 | Msa0253130:CDS | 55.0% | |
| GTAACTGCCCCACCACCAAT+GGG | + | chr2_2:44755630-44755649 | Msa0253130:CDS | 55.0% | |
| !! | CTGAAGGGTACCCTCAGCAA+AGG | + | chr2_2:44755673-44755692 | Msa0253130:CDS | 55.0% |
| CCCCTGCTAGTAGTTTGGTC+TGG | - | chr2_2:44755702-44755721 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_2 | gene | 44755552 | 44755788 | 44755552 | ID=Msa0253130;Name=Msa0253130 |
| chr2_2 | mRNA | 44755552 | 44755788 | 44755552 | ID=Msa0253130-mRNA-1;Parent=Msa0253130;Name=Msa0253130-mRNA-1;_AED=0.44;_eAED=0.44;_QI=0|-1|0|1|-1|1|1|0|78 |
| chr2_2 | exon | 44755552 | 44755788 | 44755552 | ID=Msa0253130-mRNA-1:exon:13754;Parent=Msa0253130-mRNA-1 |
| chr2_2 | CDS | 44755552 | 44755788 | 44755552 | ID=Msa0253130-mRNA-1:cds;Parent=Msa0253130-mRNA-1 |
| Gene Sequence |
| Protein sequence |
